stumps cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	stumps
Entrez Gene ID	41770
Full Name	CG31317 gene product from transcript CG31317-RC
Synonyms	0837/10,C,CG18485,CG31317,CG3375,DOF,Dmel\CG31317,Dof,Dof/stumps/heartbroken,Hbr,Hbr/Dof,P1740,anon-EST:CL47,anon-estC,dof,dof/hbr,dof/hbr/sms,dof1,gene C,hbr,heartbroken/dof,i21,i28,l(3)09904,l(3)S083710,sms,stumps/dof
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88C6-88C9

mRNA and Protein(s)

mRNA	Protein	Name
NM_169589.2	NP_731924.1	stumps, isoform B
NM_001275642.2	NP_001262571.1	stumps, isoform D
NM_169588.2	NP_731923.2	stumps, isoform E
NM_001104322.2	NP_001097792.1	stumps, isoform C
NM_001275643.1	NP_001262572.1	stumps, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	stumps	NP_731923.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001590	XP_003435886.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results identify a novel phosphotyrosine motif present in four copies in Dof and show that these motifs are phosphorylated and contribute to FGF signal transduction.
Title: A novel conserved phosphotyrosine motif in the Drosophila fibroblast growth factor signaling adaptor Dof with a redundant role in signal transmission.

We suggest that the developmental patterns of Heartbroken/Dof and Sprouty result in defining the domain and timing of activation of the Fibroblast growth factor receptor Heartless in specific myoblasts, thereby converting them into founder cells.
Title: Drosophila Heartless acts with Heartbroken/Dof in muscle founder differentiation.

Data show that Downstream-of-FGFR is a substrate for fibroblast growth factor receptors (FGFRs) and recruits Corkscrew, leading to FGF-induced cell migration and activation of the Ras/MAPK pathway.
Title: Downstream-of-FGFR is a fibroblast growth factor-specific scaffolding protein and recruits Corkscrew upon receptor activation.

identified a region within the N terminus of the protein that is required for FGF-dependent signal transduction and is necessary for efficient interaction of Dof with the FGF receptor Heartless
Title: A functional domain of Dof that is required for fibroblast growth factor signaling.

We have identified 44 proteins that interact with Dof in a yeast two-hybrid screen.
Title: Isolation of proteins that interact with the signal transduction molecule Dof and identification of a functional domain conserved between Dof and vertebrate BCAP.

The following stumps gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the stumps cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07989	NM_001275643.1 Latest version!	Drosophila melanogaster stumps, transcript variant F (stumps), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa04881	NM_001104322.2 Latest version!	Drosophila melanogaster stumps, transcript variant C (stumps), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa04882	NM_169588.2 Latest version!	Drosophila melanogaster stumps, transcript variant E (stumps), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa04883	NM_169589.2 Latest version!	Drosophila melanogaster stumps, transcript variant B (stumps), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa07988	NM_001275642.2 Latest version!	Drosophila melanogaster stumps, transcript variant D (stumps), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

stumps ( NM_001275643.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262572.1 Drosophila melanogaster stumps, transcript variant F (stumps), mRNA.

Price & Availability↑

CloneID	OFa07989
Clone ID Related Accession (Same CDS sequence)	NM_001275643.1
Accession Version	NM_001275643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stumps, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGACA TCCTGATCAT CACCGCCAAG CACAGCGAGC GCGCCTATTT GAGGGCCACT 
TTCCTGAAGA ACCACTTCGA CAAGATCACC AAACAGCGCG GACGGAAACC ATTCAATTTT 
CTGCACATCA AAATTGACGA TGGTCCCTTC AGCGAGGAGA TTGCCCACAA GTATCAGAAC 
ACAGCTTTGC AGATTGTGAT CCTCTGTCCC GCACTTTTGG CCCTTCCTCA CAGCTTTCTG 
ATGACCCAGC TATCGGCCAT TATTCGCGTG GAAAAGGTAC TGGCCATTTT GTTGGATGTC 
AGCGAGGAGA AGGTGAAGGA GATGCACAAG TCGGCACTGC CCAGTTACTA CAAGTGGCGT 
CGTTGTGTGG TGCGCGACAA TGACCAGGTG CAGATCAGCA ATATCCTGGG CATTGCCACC 
GATATCCTGG GACGCGCGTT GTGCCAGCGA CCACCGTGCC AGGATAGCTC TGGCGGAGGA 
GGTGGTGGTC TCTCGCGCAG CTTCTCTAGC TGCACCGAGG GATTCACCGT GCTGCCCAAG 
AAGGTCAAGC TGGGCCAAAA CAAGGTGGTT GCTCTGCTGG CAGAACCGCT GGAGAAGAAC 
GACACCCTGA AGCTGCAGGT GGAGAAGTCC GGTGAGCTAA TCGAGCTGCG CAACTTCAAG 
TGCCGCAATC CCTACACCCT GCAGTTCAAT ATCCCAGAGG CCTGCATGGA GATCTCCACC 
ATGATCGAGA TTCGCATCGA GAAGAACAAC AAGAGTCTGG GTGCCAGGCC CATCAAGTGC 
GAGAGTCGTC TGCGGGAACT GGAGCAGCTG CTGCGTATTG AGGACTCGCC AATTGAGTTC 
ATGTGCCATG CCCTGGGAAT TGGACCCGTG GAGCGGGATG CCCTCGATCA GCACTTGCTG 
CAGTGCTTCC AACGCAACAT GCCACCCAAC TTCCACCTGC TGAGTGGTCC CAGCGAGCGT 
CAGCCACACT TCTTCACCCG TCTGGAATCC AGTCCAGAGG AGTACCCCAC ATTGCTGCAC 
TTCGCCGCCA GATGGGGCCT GAATCGCCTG TGCATCCAGC TGATGGAGTG CCCAGGTGGC 
GACACCGCAT GCGGAGTAAG AAACTGCGCC GGACGCACTC CGGCCGAGTT GGCCGAACAG 
GAGGGGCATC ACAAGTTGGC CCAGAACATC GTCAGTTTCG CAGAGTTTCA CGAACTGACC 
ACCATGTACC ACTACTTCAA GGGAGTGACT CCGCCAGGCG AGAGCTGTCC CAAGCCCAGT 
GCCAATGTGG TGATTGAAGC ACATCCCGTT AAGGCCGGAA AGACCAACAA GCCACCGAAG 
GAACTGCCCA CGGCGGAGTA TATGGAGATG TCCAGTGGAT CGGAGCGGGA GAACACACCT 
GAAGTGAGGA CCAAGACGGA AACCCTTGCT ATCTCGAATC TCAACTACAT AAGTGTGGAA 
ACCGAGGACG AAAACCTGCA GGCTTCCGTG GCCGAAAAGA AGGTGGAATC TGAAAAGAAA 
CTGCCCGAGG CTGGCAATCA ACCTGGCCCG GAAAAGACCA CCAACGAATT GAGGATCAAC 
GAGCCACCCT ACTATCAGTC CAAAGAGCAG AACCAGCAGC CAAAGGAACT CAAGAACCAG 
AGCAAATCCC TGGACGAAGT GGATGCGGCG CCACTCTATC AGACCGACAA ATTCAGCCAA 
GAGTGCTCGC AACTTTTGGA AAACCAACTG GGCAATGACT ACGTACTGCA GCCCTCCAAT 
GTGCCGGTGG CCCAGGATGA TGGCAACTAT TTGTTCCAGC CATCGAATAG ACCCGTGGAG 
GAGCCCCTCC GGTTAAATCG GTGCACCAAC AGACAGCCCG GATATGGAAC ACTGAAGCGC 
AGTGCCAGCG ACGCATCCGC CGCCAGTCAC ACAAGATCCA ATGCCGACGA TGAGCTGGCC 
GAGATTATGC ACGATTTTAA GAACAACGTG CTCTCCATTC GGGACGTGGA AGTGCTGGTG 
GAACGCTGGA AGCACCGCAA CGATGTACAG CAGAGCTTCC GGGAGAAACA GGATCAGATC 
GACCAGCTGC GACGCGAGTA CGAGCGCATC CAGGAGCAGG TGAAGTCTCA TCTGAAGCGC 
GAAACGCCTT TCGAGCGGAT CAAGAAGTTC TTCTTGCGAT CCAAGACACC TGGGGGAAAC 
GACAGCCTGC TGGACGAGAC CAAGTCATCG ATAGCCACAA GCTGTAGCTT CAAGTCCGCG 
GGGGGCGTGA GCTCCAGCGC AGGAGCTGGA CGTCCCATCA GTTCGCTGAG CCTACAGAGT 
GTGTCCAGCT CGAGTTCCTC CTCCGGCAGA CTAAGCACCG GCAGCAATTG CAGTGGTGCC 
TCGCTGGGAG ATTCGGGTAC CCACTCGGAT CACGAGGATC GTCGCTTCCC GCACTGCCGG 
ATGATGGACA ACTACATGGT GCCACCGACA CCGAGACCCG TCTTTACGCC CAGCTCAACG 
CCCGGTGATG AGCGTCATCA GATTGTATTC CCCTCGCCCA TGTCCAGTTG CCAGCGCTTG 
AACACACCCA CCAGTCCCAC GGGATCCGAG CACTACCAAA TGTTCCCCTC GAACATACCC 
GTGTACGGCA GCCAGCCACT GGTTGGATCC CAGAGCAGCA ACTACCTGAA CACCATCATG 
GAGGCCAAGG AGCAGGCGGA GACACAGCAC TACCAAAACC AGAACGGAGT CACCCTGCAG 
CCCATTAAGC TGAACGAGCG ATCGAACATC ATCTACGGCA AGCTAACCAA GAGCAGCTCC 
GCCAGCGGAT GCAGCTCCTT CAAACCCAAG CAGATCCCAG TACCATGTCC CCAGGTGGGC 
AGCATCGAGG TCCAGGCCGA GGTCTACCAG AATGTGGGTG ACGTTGCTCC GGAAAAGGAC 
TCCAAGGCAG AGGAGGAAAC GCCAAACTAC ATGAACTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262572.1
CDS	141..3062
Translation	MDDILIITAKHSERAYLRATFLKNHFDKITKQRGRKPFNFLHIKIDDGPFSEEIAHKYQNTALQIVILCPALLALPHSFLMTQLSAIIRVEKVLAILLDVSEEKVKEMHKSALPSYYKWRRCVVRDNDQVQISNILGIATDILGRALCQRPPCQDSSGGGGGGLSRSFSSCTEGFTVLPKKVKLGQNKVVALLAEPLEKNDTLKLQVEKSGELIELRNFKCRNPYTLQFNIPEACMEISTMIEIRIEKNNKSLGARPIKCESRLRELEQLLRIEDSPIEFMCHALGIGPVERDALDQHLLQCFQRNMPPNFHLLSGPSERQPHFFTRLESSPEEYPTLLHFAARWGLNRLCIQLMECPGGDTACGVRNCAGRTPAELAEQEGHHKLAQNIVSFAEFHELTTMYHYFKGVTPPGESCPKPSANVVIEAHPVKAGKTNKPPKELPTAEYMEMSSGSERENTPEVRTKTETLAISNLNYISVETEDENLQASVAEKKVESEKKLPEAGNQPGPEKTTNELRINEPPYYQSKEQNQQPKELKNQSKSLDEVDAAPLYQTDKFSQECSQLLENQLGNDYVLQPSNVPVAQDDGNYLFQPSNRPVEEPLRLNRCTNRQPGYGTLKRSASDASAASHTRSNADDELAEIMHDFKNNVLSIRDVEVLVERWKHRNDVQQSFREKQDQIDQLRREYERIQEQVKSHLKRETPFERIKKFFLRSKTPGGNDSLLDETKSSIATSCSFKSAGGVSSSAGAGRPISSLSLQSVSSSSSSSGRLSTGSNCSGASLGDSGTHSDHEDRRFPHCRMMDNYMVPPTPRPVFTPSSTPGDERHQIVFPSPMSSCQRLNTPTSPTGSEHYQMFPSNIPVYGSQPLVGSQSSNYLNTIMEAKEQAETQHYQNQNGVTLQPIKLNERSNIIYGKLTKSSSASGCSSFKPKQIPVPCPQVGSIEVQAEVYQNVGDVAPEKDSKAEEETPNYMNC

Target ORF information:

RefSeq Version	NM_001275643.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stumps, transcript variant F (stumps), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275643.1

ORF Insert Sequence:

ATGGACGACA TCCTGATCAT CACCGCCAAG CACAGCGAGC GCGCCTATTT GAGGGCCACT 
TTCCTGAAGA ACCACTTCGA CAAGATCACC AAACAGCGCG GACGGAAACC ATTCAATTTT 
CTGCACATCA AAATTGACGA TGGTCCCTTC AGCGAGGAGA TTGCCCACAA GTATCAGAAC 
ACAGCTTTGC AGATTGTGAT CCTCTGTCCC GCACTTTTGG CCCTTCCTCA CAGCTTTCTG 
ATGACCCAGC TATCGGCCAT TATTCGCGTG GAAAAGGTAC TGGCCATTTT GTTGGATGTC 
AGCGAGGAGA AGGTGAAGGA GATGCACAAG TCGGCACTGC CCAGTTACTA CAAGTGGCGT 
CGTTGTGTGG TGCGCGACAA TGACCAGGTG CAGATCAGCA ATATCCTGGG CATTGCCACC 
GATATCCTGG GACGCGCGTT GTGCCAGCGA CCACCGTGCC AGGATAGCTC TGGCGGAGGA 
GGTGGTGGTC TCTCGCGCAG CTTCTCTAGC TGCACCGAGG GATTCACCGT GCTGCCCAAG 
AAGGTCAAGC TGGGCCAAAA CAAGGTGGTT GCTCTGCTGG CAGAACCGCT GGAGAAGAAC 
GACACCCTGA AGCTGCAGGT GGAGAAGTCC GGTGAGCTAA TCGAGCTGCG CAACTTCAAG 
TGCCGCAATC CCTACACCCT GCAGTTCAAT ATCCCAGAGG CCTGCATGGA GATCTCCACC 
ATGATCGAGA TTCGCATCGA GAAGAACAAC AAGAGTCTGG GTGCCAGGCC CATCAAGTGC 
GAGAGTCGTC TGCGGGAACT GGAGCAGCTG CTGCGTATTG AGGACTCGCC AATTGAGTTC 
ATGTGCCATG CCCTGGGAAT TGGACCCGTG GAGCGGGATG CCCTCGATCA GCACTTGCTG 
CAGTGCTTCC AACGCAACAT GCCACCCAAC TTCCACCTGC TGAGTGGTCC CAGCGAGCGT 
CAGCCACACT TCTTCACCCG TCTGGAATCC AGTCCAGAGG AGTACCCCAC ATTGCTGCAC 
TTCGCCGCCA GATGGGGCCT GAATCGCCTG TGCATCCAGC TGATGGAGTG CCCAGGTGGC 
GACACCGCAT GCGGAGTAAG AAACTGCGCC GGACGCACTC CGGCCGAGTT GGCCGAACAG 
GAGGGGCATC ACAAGTTGGC CCAGAACATC GTCAGTTTCG CAGAGTTTCA CGAACTGACC 
ACCATGTACC ACTACTTCAA GGGAGTGACT CCGCCAGGCG AGAGCTGTCC CAAGCCCAGT 
GCCAATGTGG TGATTGAAGC ACATCCCGTT AAGGCCGGAA AGACCAACAA GCCACCGAAG 
GAACTGCCCA CGGCGGAGTA TATGGAGATG TCCAGTGGAT CGGAGCGGGA GAACACACCT 
GAAGTGAGGA CCAAGACGGA AACCCTTGCT ATCTCGAATC TCAACTACAT AAGTGTGGAA 
ACCGAGGACG AAAACCTGCA GGCTTCCGTG GCCGAAAAGA AGGTGGAATC TGAAAAGAAA 
CTGCCCGAGG CTGGCAATCA ACCTGGCCCG GAAAAGACCA CCAACGAATT GAGGATCAAC 
GAGCCACCCT ACTATCAGTC CAAAGAGCAG AACCAGCAGC CAAAGGAACT CAAGAACCAG 
AGCAAATCCC TGGACGAAGT GGATGCGGCG CCACTCTATC AGACCGACAA ATTCAGCCAA 
GAGTGCTCGC AACTTTTGGA AAACCAACTG GGCAATGACT ACGTACTGCA GCCCTCCAAT 
GTGCCGGTGG CCCAGGATGA TGGCAACTAT TTGTTCCAGC CATCGAATAG ACCCGTGGAG 
GAGCCCCTCC GGTTAAATCG GTGCACCAAC AGACAGCCCG GATATGGAAC ACTGAAGCGC 
AGTGCCAGCG ACGCATCCGC CGCCAGTCAC ACAAGATCCA ATGCCGACGA TGAGCTGGCC 
GAGATTATGC ACGATTTTAA GAACAACGTG CTCTCCATTC GGGACGTGGA AGTGCTGGTG 
GAACGCTGGA AGCACCGCAA CGATGTACAG CAGAGCTTCC GGGAGAAACA GGATCAGATC 
GACCAGCTGC GACGCGAGTA CGAGCGCATC CAGGAGCAGG TGAAGTCTCA TCTGAAGCGC 
GAAACGCCTT TCGAGCGGAT CAAGAAGTTC TTCTTGCGAT CCAAGACACC TGGGGGAAAC 
GACAGCCTGC TGGACGAGAC CAAGTCATCG ATAGCCACAA GCTGTAGCTT CAAGTCCGCG 
GGGGGCGTGA GCTCCAGCGC AGGAGCTGGA CGTCCCATCA GTTCGCTGAG CCTACAGAGT 
GTGTCCAGCT CGAGTTCCTC CTCCGGCAGA CTAAGCACCG GCAGCAATTG CAGTGGTGCC 
TCGCTGGGAG ATTCGGGTAC CCACTCGGAT CACGAGGATC GTCGCTTCCC GCACTGCCGG 
ATGATGGACA ACTACATGGT GCCACCGACA CCGAGACCCG TCTTTACGCC CAGCTCAACG 
CCCGGTGATG AGCGTCATCA GATTGTATTC CCCTCGCCCA TGTCCAGTTG CCAGCGCTTG 
AACACACCCA CCAGTCCCAC GGGATCCGAG CACTACCAAA TGTTCCCCTC GAACATACCC 
GTGTACGGCA GCCAGCCACT GGTTGGATCC CAGAGCAGCA ACTACCTGAA CACCATCATG 
GAGGCCAAGG AGCAGGCGGA GACACAGCAC TACCAAAACC AGAACGGAGT CACCCTGCAG 
CCCATTAAGC TGAACGAGCG ATCGAACATC ATCTACGGCA AGCTAACCAA GAGCAGCTCC 
GCCAGCGGAT GCAGCTCCTT CAAACCCAAG CAGATCCCAG TACCATGTCC CCAGGTGGGC 
AGCATCGAGG TCCAGGCCGA GGTCTACCAG AATGTGGGTG ACGTTGCTCC GGAAAAGGAC 
TCCAAGGCAG AGGAGGAAAC GCCAAACTAC ATGAACTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stumps ( NM_001104322.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097792.1 Drosophila melanogaster stumps, transcript variant C (stumps), mRNA.

Price & Availability↑

CloneID	OFa04881
Clone ID Related Accession (Same CDS sequence)	NM_001104322.2
Accession Version	NM_001104322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stumps, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104322.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGTGG ACAACCCGTA TTACTTTTGC AATGAAACCA AGTCGCCGCG CACCCCGGAC 
TCGCAGTCCT CCAGCCGGGG ATTCTCGATC TTCAAGCGGC GCAACTCGAA GGGCAGCAGC 
CCCCGGCCGC TGGTGATGAC CAATCCGGAA CAGATGCGAC TGATCACATC CGCCAATCTG 
GATACCACGG CTACCATGGC ATTAGGAAGT CCGCGGGGCA GCTTCAATTC CCTCGCCGGC 
TATCCGCAAC ACGCGATTGA ACTGCAGACG ATGAACATGT ACCGCCAGCG ATCTGCAAAT 
TCGCACTCAG AGTTCCGGGA ACGCCTGAAT TCTTGCAACG AGACCCACAG CTCCCAGTGT 
CCCGGAGGAG GAGGTGCATC CGTAGCTGGG GATCACGACG AGGTCTTCAA TCCGCTGACA 
TCGCCAACAA GTAGCAAATC GCATCGACAT CACACCCTGC CAAGTGGCGG AACTCGTCGC 
CATTCCCTGG GAAACTGGGC CCAACATCAG CATCAACAGC AACATCAACA GAACTCTGGA 
GCAATGGGAC TGGGATCTCC GGATGAGGTG CCCAGCATGA TGGCGCCTCG ACCGCCGCTC 
CTCTCGCCAA ACAAATTCCG CGGCAGTTCC CATCGACGAA GAGACAACTG CGGCAACGTG 
AACAACACGA GTGGATCGAG TGCATCCGGA TTCCTGGGCT CTCCCGGCTA CAACATGGAC 
GACATCCTGA TCATCACCGC CAAGCACAGC GAGCGCGCCT ATTTGAGGGC CACTTTCCTG 
AAGAACCACT TCGACAAGAT CACCAAACAG CGCGGACGGA AACCATTCAA TTTTCTGCAC 
ATCAAAATTG ACGATGGTCC CTTCAGCGAG GAGATTGCCC ACAAGTATCA GAACACAGCT 
TTGCAGATTG TGATCCTCTG TCCCGCACTT TTGGCCCTTC CTCACAGCTT TCTGATGACC 
CAGCTATCGG CCATTATTCG CGTGGAAAAG GTACTGGCCA TTTTGTTGGA TGTCAGCGAG 
GAGAAGGTGA AGGAGATGCA CAAGTCGGCA CTGCCCAGTT ACTACAAGTG GCGTCGTTGT 
GTGGTGCGCG ACAATGACCA GGTGCAGATC AGCAATATCC TGGGCATTGC CACCGATATC 
CTGGGACGCG CGTTGTGCCA GCGACCACCG TGCCAGGATA GCTCTGGCGG AGGAGGTGGT 
GGTCTCTCGC GCAGCTTCTC TAGCTGCACC GAGGGATTCA CCGTGCTGCC CAAGAAGGTC 
AAGCTGGGCC AAAACAAGGT GGTTGCTCTG CTGGCAGAAC CGCTGGAGAA GAACGACACC 
CTGAAGCTGC AGGTGGAGAA GTCCGGTGAG CTAATCGAGC TGCGCAACTT CAAGTGCCGC 
AATCCCTACA CCCTGCAGTT CAATATCCCA GAGGCCTGCA TGGAGATCTC CACCATGATC 
GAGATTCGCA TCGAGAAGAA CAACAAGAGT CTGGGTGCCA GGCCCATCAA GTGCGAGAGT 
CGTCTGCGGG AACTGGAGCA GCTGCTGCGT ATTGAGGACT CGCCAATTGA GTTCATGTGC 
CATGCCCTGG GAATTGGACC CGTGGAGCGG GATGCCCTCG ATCAGCACTT GCTGCAGTGC 
TTCCAACGCA ACATGCCACC CAACTTCCAC CTGCTGAGTG GTCCCAGCGA GCGTCAGCCA 
CACTTCTTCA CCCGTCTGGA ATCCAGTCCA GAGGAGTACC CCACATTGCT GCACTTCGCC 
GCCAGATGGG GCCTGAATCG CCTGTGCATC CAGCTGATGG AGTGCCCAGG TGGCGACACC 
GCATGCGGAG TAAGAAACTG CGCCGGACGC ACTCCGGCCG AGTTGGCCGA ACAGGAGGGG 
CATCACAAGT TGGCCCAGAA CATCGTCAGT TTCGCAGAGT TTCACGAACT GACCACCATG 
TACCACTACT TCAAGGGAGT GACTCCGCCA GGCGAGAGCT GTCCCAAGCC CAGTGCCAAT 
GTGGTGATTG AAGCACATCC CGTTAAGGCC GGAAAGACCA ACAAGCCACC GAAGGAACTG 
CCCACGGCGG AGTATATGGA GATGTCCAGT GGATCGGAGC GGGAGAACAC ACCTGAAGTG 
AGGACCAAGA CGGAAACCCT TGCTATCTCG AATCTCAACT ACATAAGTGT GGAAACCGAG 
GACGAAAACC TGCAGGCTTC CGTGGCCGAA AAGAAGGTGG AATCTGAAAA GAAACTGCCC 
GAGGCTGGCA ATCAACCTGG CCCGGAAAAG ACCACCAACG AATTGAGGAT CAACGAGCCA 
CCCTACTATC AGTCCAAAGA GCAGAACCAG CAGCCAAAGG AACTCAAGAA CCAGAGCAAA 
TCCCTGGACG AAGTGGATGC GGCGCCACTC TATCAGACCG ACAAATTCAG CCAAGAGTGC 
TCGCAACTTT TGGAAAACCA ACTGGGCAAT GACTACGTAC TGCAGCCCTC CAATGTGCCG 
GTGGCCCAGG ATGATGGCAA CTATTTGTTC CAGCCATCGA ATAGACCCGT GGAGGAGCCC 
CTCCGGTTAA ATCGGTGCAC CAACAGACAG CCCGGATATG GAACACTGAA GCGCAGTGCC 
AGCGACGCAT CCGCCGCCAG TCACACAAGA TCCAATGCCG ACGATGAGCT GGCCGAGATT 
ATGCACGATT TTAAGAACAA CGTGCTCTCC ATTCGGGACG TGGAAGTGCT GGTGGAACGC 
TGGAAGCACC GCAACGATGT ACAGCAGAGC TTCCGGGAGA AACAGGATCA GATCGACCAG 
CTGCGACGCG AGTACGAGCG CATCCAGGAG CAGGTGAAGT CTCATCTGAA GCGCGAAACG 
CCTTTCGAGC GGATCAAGAA GTTCTTCTTG CGATCCAAGA CACCTGGGGG AAACGACAGC 
CTGCTGGACG AGACCAAGTC ATCGATAGCC ACAAGCTGTA GCTTCAAGTC CGCGGGGGGC 
GTGAGCTCCA GCGCAGGAGC TGGACGTCCC ATCAGTTCGC TGAGCCTACA GAGTGTGTCC 
AGCTCGAGTT CCTCCTCCGG CAGACTAAGC ACCGGCAGCA ATTGCAGTGG TGCCTCGCTG 
GGAGATTCGG GTACCCACTC GGATCACGAG GATCGTCGCT TCCCGCACTG CCGGATGATG 
GACAACTACA TGGTGCCACC GACACCGAGA CCCGTCTTTA CGCCCAGCTC AACGCCCGGT 
GATGAGCGTC ATCAGATTGT ATTCCCCTCG CCCATGTCCA GTTGCCAGCG CTTGAACACA 
CCCACCAGTC CCACGGGATC CGAGCACTAC CAAATGTTCC CCTCGAACAT ACCCGTGTAC 
GGCAGCCAGC CACTGGTTGG ATCCCAGAGC AGCAACTACC TGAACACCAT CATGGAGGCC 
AAGGAGCAGG CGGAGACACA GCACTACCAA AACCAGAACG GAGTCACCCT GCAGCCCATT 
AAGCTGAACG AGCGATCGAA CATCATCTAC GGCAAGCTAA CCAAGAGCAG CTCCGCCAGC 
GGATGCAGCT CCTTCAAACC CAAGCAGATC CCAGTACCAT GTCCCCAGGT GGGCAGCATC 
GAGGTCCAGG CCGAGGTCTA CCAGAATGTG GGTGACGTTG CTCCGGAAAA GGACTCCAAG 
GCAGAGGAGG AAACGCCAAA CTACATGAAC TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097792.1
CDS	463..4098
Translation	MSVDNPYYFCNETKSPRTPDSQSSSRGFSIFKRRNSKGSSPRPLVMTNPEQMRLITSANLDTTATMALGSPRGSFNSLAGYPQHAIELQTMNMYRQRSANSHSEFRERLNSCNETHSSQCPGGGGASVAGDHDEVFNPLTSPTSSKSHRHHTLPSGGTRRHSLGNWAQHQHQQQHQQNSGAMGLGSPDEVPSMMAPRPPLLSPNKFRGSSHRRRDNCGNVNNTSGSSASGFLGSPGYNMDDILIITAKHSERAYLRATFLKNHFDKITKQRGRKPFNFLHIKIDDGPFSEEIAHKYQNTALQIVILCPALLALPHSFLMTQLSAIIRVEKVLAILLDVSEEKVKEMHKSALPSYYKWRRCVVRDNDQVQISNILGIATDILGRALCQRPPCQDSSGGGGGGLSRSFSSCTEGFTVLPKKVKLGQNKVVALLAEPLEKNDTLKLQVEKSGELIELRNFKCRNPYTLQFNIPEACMEISTMIEIRIEKNNKSLGARPIKCESRLRELEQLLRIEDSPIEFMCHALGIGPVERDALDQHLLQCFQRNMPPNFHLLSGPSERQPHFFTRLESSPEEYPTLLHFAARWGLNRLCIQLMECPGGDTACGVRNCAGRTPAELAEQEGHHKLAQNIVSFAEFHELTTMYHYFKGVTPPGESCPKPSANVVIEAHPVKAGKTNKPPKELPTAEYMEMSSGSERENTPEVRTKTETLAISNLNYISVETEDENLQASVAEKKVESEKKLPEAGNQPGPEKTTNELRINEPPYYQSKEQNQQPKELKNQSKSLDEVDAAPLYQTDKFSQECSQLLENQLGNDYVLQPSNVPVAQDDGNYLFQPSNRPVEEPLRLNRCTNRQPGYGTLKRSASDASAASHTRSNADDELAEIMHDFKNNVLSIRDVEVLVERWKHRNDVQQSFREKQDQIDQLRREYERIQEQVKSHLKRETPFERIKKFFLRSKTPGGNDSLLDETKSSIATSCSFKSAGGVSSSAGAGRPISSLSLQSVSSSSSSSGRLSTGSNCSGASLGDSGTHSDHEDRRFPHCRMMDNYMVPPTPRPVFTPSSTPGDERHQIVFPSPMSSCQRLNTPTSPTGSEHYQMFPSNIPVYGSQPLVGSQSSNYLNTIMEAKEQAETQHYQNQNGVTLQPIKLNERSNIIYGKLTKSSSASGCSSFKPKQIPVPCPQVGSIEVQAEVYQNVGDVAPEKDSKAEEETPNYMNC

Target ORF information:

RefSeq Version	NM_001104322.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stumps, transcript variant C (stumps), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104322.2

ORF Insert Sequence:

ATGTCTGTGG ACAACCCGTA TTACTTTTGC AATGAAACCA AGTCGCCGCG CACCCCGGAC 
TCGCAGTCCT CCAGCCGGGG ATTCTCGATC TTCAAGCGGC GCAACTCGAA GGGCAGCAGC 
CCCCGGCCGC TGGTGATGAC CAATCCGGAA CAGATGCGAC TGATCACATC CGCCAATCTG 
GATACCACGG CTACCATGGC ATTAGGAAGT CCGCGGGGCA GCTTCAATTC CCTCGCCGGC 
TATCCGCAAC ACGCGATTGA ACTGCAGACG ATGAACATGT ACCGCCAGCG ATCTGCAAAT 
TCGCACTCAG AGTTCCGGGA ACGCCTGAAT TCTTGCAACG AGACCCACAG CTCCCAGTGT 
CCCGGAGGAG GAGGTGCATC CGTAGCTGGG GATCACGACG AGGTCTTCAA TCCGCTGACA 
TCGCCAACAA GTAGCAAATC GCATCGACAT CACACCCTGC CAAGTGGCGG AACTCGTCGC 
CATTCCCTGG GAAACTGGGC CCAACATCAG CATCAACAGC AACATCAACA GAACTCTGGA 
GCAATGGGAC TGGGATCTCC GGATGAGGTG CCCAGCATGA TGGCGCCTCG ACCGCCGCTC 
CTCTCGCCAA ACAAATTCCG CGGCAGTTCC CATCGACGAA GAGACAACTG CGGCAACGTG 
AACAACACGA GTGGATCGAG TGCATCCGGA TTCCTGGGCT CTCCCGGCTA CAACATGGAC 
GACATCCTGA TCATCACCGC CAAGCACAGC GAGCGCGCCT ATTTGAGGGC CACTTTCCTG 
AAGAACCACT TCGACAAGAT CACCAAACAG CGCGGACGGA AACCATTCAA TTTTCTGCAC 
ATCAAAATTG ACGATGGTCC CTTCAGCGAG GAGATTGCCC ACAAGTATCA GAACACAGCT 
TTGCAGATTG TGATCCTCTG TCCCGCACTT TTGGCCCTTC CTCACAGCTT TCTGATGACC 
CAGCTATCGG CCATTATTCG CGTGGAAAAG GTACTGGCCA TTTTGTTGGA TGTCAGCGAG 
GAGAAGGTGA AGGAGATGCA CAAGTCGGCA CTGCCCAGTT ACTACAAGTG GCGTCGTTGT 
GTGGTGCGCG ACAATGACCA GGTGCAGATC AGCAATATCC TGGGCATTGC CACCGATATC 
CTGGGACGCG CGTTGTGCCA GCGACCACCG TGCCAGGATA GCTCTGGCGG AGGAGGTGGT 
GGTCTCTCGC GCAGCTTCTC TAGCTGCACC GAGGGATTCA CCGTGCTGCC CAAGAAGGTC 
AAGCTGGGCC AAAACAAGGT GGTTGCTCTG CTGGCAGAAC CGCTGGAGAA GAACGACACC 
CTGAAGCTGC AGGTGGAGAA GTCCGGTGAG CTAATCGAGC TGCGCAACTT CAAGTGCCGC 
AATCCCTACA CCCTGCAGTT CAATATCCCA GAGGCCTGCA TGGAGATCTC CACCATGATC 
GAGATTCGCA TCGAGAAGAA CAACAAGAGT CTGGGTGCCA GGCCCATCAA GTGCGAGAGT 
CGTCTGCGGG AACTGGAGCA GCTGCTGCGT ATTGAGGACT CGCCAATTGA GTTCATGTGC 
CATGCCCTGG GAATTGGACC CGTGGAGCGG GATGCCCTCG ATCAGCACTT GCTGCAGTGC 
TTCCAACGCA ACATGCCACC CAACTTCCAC CTGCTGAGTG GTCCCAGCGA GCGTCAGCCA 
CACTTCTTCA CCCGTCTGGA ATCCAGTCCA GAGGAGTACC CCACATTGCT GCACTTCGCC 
GCCAGATGGG GCCTGAATCG CCTGTGCATC CAGCTGATGG AGTGCCCAGG TGGCGACACC 
GCATGCGGAG TAAGAAACTG CGCCGGACGC ACTCCGGCCG AGTTGGCCGA ACAGGAGGGG 
CATCACAAGT TGGCCCAGAA CATCGTCAGT TTCGCAGAGT TTCACGAACT GACCACCATG 
TACCACTACT TCAAGGGAGT GACTCCGCCA GGCGAGAGCT GTCCCAAGCC CAGTGCCAAT 
GTGGTGATTG AAGCACATCC CGTTAAGGCC GGAAAGACCA ACAAGCCACC GAAGGAACTG 
CCCACGGCGG AGTATATGGA GATGTCCAGT GGATCGGAGC GGGAGAACAC ACCTGAAGTG 
AGGACCAAGA CGGAAACCCT TGCTATCTCG AATCTCAACT ACATAAGTGT GGAAACCGAG 
GACGAAAACC TGCAGGCTTC CGTGGCCGAA AAGAAGGTGG AATCTGAAAA GAAACTGCCC 
GAGGCTGGCA ATCAACCTGG CCCGGAAAAG ACCACCAACG AATTGAGGAT CAACGAGCCA 
CCCTACTATC AGTCCAAAGA GCAGAACCAG CAGCCAAAGG AACTCAAGAA CCAGAGCAAA 
TCCCTGGACG AAGTGGATGC GGCGCCACTC TATCAGACCG ACAAATTCAG CCAAGAGTGC 
TCGCAACTTT TGGAAAACCA ACTGGGCAAT GACTACGTAC TGCAGCCCTC CAATGTGCCG 
GTGGCCCAGG ATGATGGCAA CTATTTGTTC CAGCCATCGA ATAGACCCGT GGAGGAGCCC 
CTCCGGTTAA ATCGGTGCAC CAACAGACAG CCCGGATATG GAACACTGAA GCGCAGTGCC 
AGCGACGCAT CCGCCGCCAG TCACACAAGA TCCAATGCCG ACGATGAGCT GGCCGAGATT 
ATGCACGATT TTAAGAACAA CGTGCTCTCC ATTCGGGACG TGGAAGTGCT GGTGGAACGC 
TGGAAGCACC GCAACGATGT ACAGCAGAGC TTCCGGGAGA AACAGGATCA GATCGACCAG 
CTGCGACGCG AGTACGAGCG CATCCAGGAG CAGGTGAAGT CTCATCTGAA GCGCGAAACG 
CCTTTCGAGC GGATCAAGAA GTTCTTCTTG CGATCCAAGA CACCTGGGGG AAACGACAGC 
CTGCTGGACG AGACCAAGTC ATCGATAGCC ACAAGCTGTA GCTTCAAGTC CGCGGGGGGC 
GTGAGCTCCA GCGCAGGAGC TGGACGTCCC ATCAGTTCGC TGAGCCTACA GAGTGTGTCC 
AGCTCGAGTT CCTCCTCCGG CAGACTAAGC ACCGGCAGCA ATTGCAGTGG TGCCTCGCTG 
GGAGATTCGG GTACCCACTC GGATCACGAG GATCGTCGCT TCCCGCACTG CCGGATGATG 
GACAACTACA TGGTGCCACC GACACCGAGA CCCGTCTTTA CGCCCAGCTC AACGCCCGGT 
GATGAGCGTC ATCAGATTGT ATTCCCCTCG CCCATGTCCA GTTGCCAGCG CTTGAACACA 
CCCACCAGTC CCACGGGATC CGAGCACTAC CAAATGTTCC CCTCGAACAT ACCCGTGTAC 
GGCAGCCAGC CACTGGTTGG ATCCCAGAGC AGCAACTACC TGAACACCAT CATGGAGGCC 
AAGGAGCAGG CGGAGACACA GCACTACCAA AACCAGAACG GAGTCACCCT GCAGCCCATT 
AAGCTGAACG AGCGATCGAA CATCATCTAC GGCAAGCTAA CCAAGAGCAG CTCCGCCAGC 
GGATGCAGCT CCTTCAAACC CAAGCAGATC CCAGTACCAT GTCCCCAGGT GGGCAGCATC 
GAGGTCCAGG CCGAGGTCTA CCAGAATGTG GGTGACGTTG CTCCGGAAAA GGACTCCAAG 
GCAGAGGAGG AAACGCCAAA CTACATGAAC TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stumps ( NM_169588.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731923.2 Drosophila melanogaster stumps, transcript variant E (stumps), mRNA.

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CloneID	OFa04882
Clone ID Related Accession (Same CDS sequence)	NM_169588.2
Accession Version	NM_169588.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stumps, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169588.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGTCA CTGTGGATAA CACAAACACC TTCTTCTCAG ACAACCCGTA TTACTTTTGC 
AATGAAACCA AGTCGCCGCG CACCCCGGAC TCGCAGTCCT CCAGCCGGGG ATTCTCGATC 
TTCAAGCGGC GCAACTCGAA GGGCAGCAGC CCCCGGCCGC TGGTGATGAC CAATCCGGAA 
CAGATGCGAC TGATCACATC CGCCAATCTG GATACCACGG CTACCATGGC ATTAGGAAGT 
CCGCGGGGCA GCTTCAATTC CCTCGCCGGC TATCCGCAAC ACGCGATTGA ACTGCAGACG 
ATGAACATGT ACCGCCAGCG ATCTGCAAAT TCGCACTCAG AGTTCCGGGA ACGCCTGAAT 
TCTTGCAACG AGACCCACAG CTCCCAGTGT CCCGGAGGAG GAGGTGCATC CGTAGCTGGG 
GATCACGACG AGGTCTTCAA TCCGCTGACA TCGCCAACAA GCAAATCGCA TCGACATCAC 
ACCCTGCCAA GTGGCGGAAC TCGTCGCCAT TCCCTGGGAA ACTGGGCCCA ACATCAGCAT 
CAACAGCAAC ATCAACAGAA CTCTGGAGCA ATGGGACTGG GATCTCCGGA TGAGGTGCCC 
AGCATGATGG CGCCTCGACC GCCGCTCCTC TCGCCAAACA AATTCCGCGG CAGTTCCCAT 
CGACGAAGAG ACAACTGCGG CAACGTGAAC AACACGAGTG GATCGAGTGC ATCCGGATTC 
CTGGGCTCTC CCGGCTACAA CATGGACGAC ATCCTGATCA TCACCGCCAA GCACAGCGAG 
CGCGCCTATT TGAGGGCCAC TTTCCTGAAG AACCACTTCG ACAAGATCAC CAAACAGCGC 
GGACGGAAAC CATTCAATTT TCTGCACATC AAAATTGACG ATGGTCCCTT CAGCGAGGAG 
ATTGCCCACA AGTATCAGAA CACAGCTTTG CAGATTGTGA TCCTCTGTCC CGCACTTTTG 
GCCCTTCCTC ACAGCTTTCT GATGACCCAG CTATCGGCCA TTATTCGCGT GGAAAAGGTA 
CTGGCCATTT TGTTGGATGT CAGCGAGGAG AAGGTGAAGG AGATGCACAA GTCGGCACTG 
CCCAGTTACT ACAAGTGGCG TCGTTGTGTG GTGCGCGACA ATGACCAGGT GCAGATCAGC 
AATATCCTGG GCATTGCCAC CGATATCCTG GGACGCGCGT TGTGCCAGCG ACCACCGTGC 
CAGGATAGCT CTGGCGGAGG AGGTGGTGGT CTCTCGCGCA GCTTCTCTAG CTGCACCGAG 
GGATTCACCG TGCTGCCCAA GAAGGTCAAG CTGGGCCAAA ACAAGGTGGT TGCTCTGCTG 
GCAGAACCGC TGGAGAAGAA CGACACCCTG AAGCTGCAGG TGGAGAAGTC CGGTGAGCTA 
ATCGAGCTGC GCAACTTCAA GTGCCGCAAT CCCTACACCC TGCAGTTCAA TATCCCAGAG 
GCCTGCATGG AGATCTCCAC CATGATCGAG ATTCGCATCG AGAAGAACAA CAAGAGTCTG 
GGTGCCAGGC CCATCAAGTG CGAGAGTCGT CTGCGGGAAC TGGAGCAGCT GCTGCGTATT 
GAGGACTCGC CAATTGAGTT CATGTGCCAT GCCCTGGGAA TTGGACCCGT GGAGCGGGAT 
GCCCTCGATC AGCACTTGCT GCAGTGCTTC CAACGCAACA TGCCACCCAA CTTCCACCTG 
CTGAGTGGTC CCAGCGAGCG TCAGCCACAC TTCTTCACCC GTCTGGAATC CAGTCCAGAG 
GAGTACCCCA CATTGCTGCA CTTCGCCGCC AGATGGGGCC TGAATCGCCT GTGCATCCAG 
CTGATGGAGT GCCCAGGTGG CGACACCGCA TGCGGAGTAA GAAACTGCGC CGGACGCACT 
CCGGCCGAGT TGGCCGAACA GGAGGGGCAT CACAAGTTGG CCCAGAACAT CGTCAGTTTC 
GCAGAGTTTC ACGAACTGAC CACCATGTAC CACTACTTCA AGGGAGTGAC TCCGCCAGGC 
GAGAGCTGTC CCAAGCCCAG TGCCAATGTG GTGATTGAAG CACATCCCGT TAAGGCCGGA 
AAGACCAACA AGCCACCGAA GGAACTGCCC ACGGCGGAGT ATATGGAGAT GTCCAGTGGA 
TCGGAGCGGG AGAACACACC TGAAGTGAGG ACCAAGACGG AAACCCTTGC TATCTCGAAT 
CTCAACTACA TAAGTGTGGA AACCGAGGAC GAAAACCTGC AGGCTTCCGT GGCCGAAAAG 
AAGGTGGAAT CTGAAAAGAA ACTGCCCGAG GCTGGCAATC AACCTGGCCC GGAAAAGACC 
ACCAACGAAT TGAGGATCAA CGAGCCACCC TACTATCAGT CCAAAGAGCA GAACCAGCAG 
CCAAAGGAAC TCAAGAACCA GAGCAAATCC CTGGACGAAG TGGATGCGGC GCCACTCTAT 
CAGACCGACA AATTCAGCCA AGAGTGCTCG CAACTTTTGG AAAACCAACT GGGCAATGAC 
TACGTACTGC AGCCCTCCAA TGTGCCGGTG GCCCAGGATG ATGGCAACTA TTTGTTCCAG 
CCATCGAATA GACCCGTGGA GGAGCCCCTC CGGTTAAATC GGTGCACCAA CAGACAGCCC 
GGATATGGAA CACTGAAGCG CAGTGCCAGC GACGCATCCG CCGCCAGTCA CACAAGATCC 
AATGCCGACG ATGAGCTGGC CGAGATTATG CACGATTTTA AGAACAACGT GCTCTCCATT 
CGGGACGTGG AAGTGCTGGT GGAACGCTGG AAGCACCGCA ACGATGTACA GCAGAGCTTC 
CGGGAGAAAC AGGATCAGAT CGACCAGCTG CGACGCGAGT ACGAGCGCAT CCAGGAGCAG 
GTGAAGTCTC ATCTGAAGCG CGAAACGCCT TTCGAGCGGA TCAAGAAGTT CTTCTTGCGA 
TCCAAGACAC CTGGGGGAAA CGACAGCCTG CTGGACGAGA CCAAGTCATC GATAGCCACA 
AGCTGTAGCT TCAAGTCCGC GGGGGGCGTG AGCTCCAGCG CAGGAGCTGG ACGTCCCATC 
AGTTCGCTGA GCCTACAGAG TGTGTCCAGC TCGAGTTCCT CCTCCGGCAG ACTAAGCACC 
GGCAGCAATT GCAGTGGTGC CTCGCTGGGA GATTCGGGTA CCCACTCGGA TCACGAGGAT 
CGTCGCTTCC CGCACTGCCG GATGATGGAC AACTACATGG TGCCACCGAC ACCGAGACCC 
GTCTTTACGC CCAGCTCAAC GCCCGGTGAT GAGCGTCATC AGATTGTATT CCCCTCGCCC 
ATGTCCAGTT GCCAGCGCTT GAACACACCC ACCAGTCCCA CGGGATCCGA GCACTACCAA 
ATGTTCCCCT CGAACATACC CGTGTACGGC AGCCAGCCAC TGGTTGGATC CCAGAGCAGC 
AACTACCTGA ACACCATCAT GGAGGCCAAG GAGCAGGCGG AGACACAGCA CTACCAAAAC 
CAGAACGGAG TCACCCTGCA GCCCATTAAG CTGAACGAGC GATCGAACAT CATCTACGGC 
AAGCTAACCA AGAGCAGCTC CGCCAGCGGA TGCAGCTCCT TCAAACCCAA GCAGATCCCA 
GTACCATGTC CCCAGGTGGG CAGCATCGAG GTCCAGGCCG AGGTCTACCA GAATGTGGGT 
GACGTTGCTC CGGAAAAGGA CTCCAAGGCA GAGGAGGAAA CGCCAAACTA CATGAACTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731923.2
CDS	351..4013
Translation	MFVTVDNTNTFFSDNPYYFCNETKSPRTPDSQSSSRGFSIFKRRNSKGSSPRPLVMTNPEQMRLITSANLDTTATMALGSPRGSFNSLAGYPQHAIELQTMNMYRQRSANSHSEFRERLNSCNETHSSQCPGGGGASVAGDHDEVFNPLTSPTSKSHRHHTLPSGGTRRHSLGNWAQHQHQQQHQQNSGAMGLGSPDEVPSMMAPRPPLLSPNKFRGSSHRRRDNCGNVNNTSGSSASGFLGSPGYNMDDILIITAKHSERAYLRATFLKNHFDKITKQRGRKPFNFLHIKIDDGPFSEEIAHKYQNTALQIVILCPALLALPHSFLMTQLSAIIRVEKVLAILLDVSEEKVKEMHKSALPSYYKWRRCVVRDNDQVQISNILGIATDILGRALCQRPPCQDSSGGGGGGLSRSFSSCTEGFTVLPKKVKLGQNKVVALLAEPLEKNDTLKLQVEKSGELIELRNFKCRNPYTLQFNIPEACMEISTMIEIRIEKNNKSLGARPIKCESRLRELEQLLRIEDSPIEFMCHALGIGPVERDALDQHLLQCFQRNMPPNFHLLSGPSERQPHFFTRLESSPEEYPTLLHFAARWGLNRLCIQLMECPGGDTACGVRNCAGRTPAELAEQEGHHKLAQNIVSFAEFHELTTMYHYFKGVTPPGESCPKPSANVVIEAHPVKAGKTNKPPKELPTAEYMEMSSGSERENTPEVRTKTETLAISNLNYISVETEDENLQASVAEKKVESEKKLPEAGNQPGPEKTTNELRINEPPYYQSKEQNQQPKELKNQSKSLDEVDAAPLYQTDKFSQECSQLLENQLGNDYVLQPSNVPVAQDDGNYLFQPSNRPVEEPLRLNRCTNRQPGYGTLKRSASDASAASHTRSNADDELAEIMHDFKNNVLSIRDVEVLVERWKHRNDVQQSFREKQDQIDQLRREYERIQEQVKSHLKRETPFERIKKFFLRSKTPGGNDSLLDETKSSIATSCSFKSAGGVSSSAGAGRPISSLSLQSVSSSSSSSGRLSTGSNCSGASLGDSGTHSDHEDRRFPHCRMMDNYMVPPTPRPVFTPSSTPGDERHQIVFPSPMSSCQRLNTPTSPTGSEHYQMFPSNIPVYGSQPLVGSQSSNYLNTIMEAKEQAETQHYQNQNGVTLQPIKLNERSNIIYGKLTKSSSASGCSSFKPKQIPVPCPQVGSIEVQAEVYQNVGDVAPEKDSKAEEETPNYMNC

Target ORF information:

RefSeq Version	NM_169588.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stumps, transcript variant E (stumps), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169588.2

ORF Insert Sequence:

ATGTTTGTCA CTGTGGATAA CACAAACACC TTCTTCTCAG ACAACCCGTA TTACTTTTGC 
AATGAAACCA AGTCGCCGCG CACCCCGGAC TCGCAGTCCT CCAGCCGGGG ATTCTCGATC 
TTCAAGCGGC GCAACTCGAA GGGCAGCAGC CCCCGGCCGC TGGTGATGAC CAATCCGGAA 
CAGATGCGAC TGATCACATC CGCCAATCTG GATACCACGG CTACCATGGC ATTAGGAAGT 
CCGCGGGGCA GCTTCAATTC CCTCGCCGGC TATCCGCAAC ACGCGATTGA ACTGCAGACG 
ATGAACATGT ACCGCCAGCG ATCTGCAAAT TCGCACTCAG AGTTCCGGGA ACGCCTGAAT 
TCTTGCAACG AGACCCACAG CTCCCAGTGT CCCGGAGGAG GAGGTGCATC CGTAGCTGGG 
GATCACGACG AGGTCTTCAA TCCGCTGACA TCGCCAACAA GCAAATCGCA TCGACATCAC 
ACCCTGCCAA GTGGCGGAAC TCGTCGCCAT TCCCTGGGAA ACTGGGCCCA ACATCAGCAT 
CAACAGCAAC ATCAACAGAA CTCTGGAGCA ATGGGACTGG GATCTCCGGA TGAGGTGCCC 
AGCATGATGG CGCCTCGACC GCCGCTCCTC TCGCCAAACA AATTCCGCGG CAGTTCCCAT 
CGACGAAGAG ACAACTGCGG CAACGTGAAC AACACGAGTG GATCGAGTGC ATCCGGATTC 
CTGGGCTCTC CCGGCTACAA CATGGACGAC ATCCTGATCA TCACCGCCAA GCACAGCGAG 
CGCGCCTATT TGAGGGCCAC TTTCCTGAAG AACCACTTCG ACAAGATCAC CAAACAGCGC 
GGACGGAAAC CATTCAATTT TCTGCACATC AAAATTGACG ATGGTCCCTT CAGCGAGGAG 
ATTGCCCACA AGTATCAGAA CACAGCTTTG CAGATTGTGA TCCTCTGTCC CGCACTTTTG 
GCCCTTCCTC ACAGCTTTCT GATGACCCAG CTATCGGCCA TTATTCGCGT GGAAAAGGTA 
CTGGCCATTT TGTTGGATGT CAGCGAGGAG AAGGTGAAGG AGATGCACAA GTCGGCACTG 
CCCAGTTACT ACAAGTGGCG TCGTTGTGTG GTGCGCGACA ATGACCAGGT GCAGATCAGC 
AATATCCTGG GCATTGCCAC CGATATCCTG GGACGCGCGT TGTGCCAGCG ACCACCGTGC 
CAGGATAGCT CTGGCGGAGG AGGTGGTGGT CTCTCGCGCA GCTTCTCTAG CTGCACCGAG 
GGATTCACCG TGCTGCCCAA GAAGGTCAAG CTGGGCCAAA ACAAGGTGGT TGCTCTGCTG 
GCAGAACCGC TGGAGAAGAA CGACACCCTG AAGCTGCAGG TGGAGAAGTC CGGTGAGCTA 
ATCGAGCTGC GCAACTTCAA GTGCCGCAAT CCCTACACCC TGCAGTTCAA TATCCCAGAG 
GCCTGCATGG AGATCTCCAC CATGATCGAG ATTCGCATCG AGAAGAACAA CAAGAGTCTG 
GGTGCCAGGC CCATCAAGTG CGAGAGTCGT CTGCGGGAAC TGGAGCAGCT GCTGCGTATT 
GAGGACTCGC CAATTGAGTT CATGTGCCAT GCCCTGGGAA TTGGACCCGT GGAGCGGGAT 
GCCCTCGATC AGCACTTGCT GCAGTGCTTC CAACGCAACA TGCCACCCAA CTTCCACCTG 
CTGAGTGGTC CCAGCGAGCG TCAGCCACAC TTCTTCACCC GTCTGGAATC CAGTCCAGAG 
GAGTACCCCA CATTGCTGCA CTTCGCCGCC AGATGGGGCC TGAATCGCCT GTGCATCCAG 
CTGATGGAGT GCCCAGGTGG CGACACCGCA TGCGGAGTAA GAAACTGCGC CGGACGCACT 
CCGGCCGAGT TGGCCGAACA GGAGGGGCAT CACAAGTTGG CCCAGAACAT CGTCAGTTTC 
GCAGAGTTTC ACGAACTGAC CACCATGTAC CACTACTTCA AGGGAGTGAC TCCGCCAGGC 
GAGAGCTGTC CCAAGCCCAG TGCCAATGTG GTGATTGAAG CACATCCCGT TAAGGCCGGA 
AAGACCAACA AGCCACCGAA GGAACTGCCC ACGGCGGAGT ATATGGAGAT GTCCAGTGGA 
TCGGAGCGGG AGAACACACC TGAAGTGAGG ACCAAGACGG AAACCCTTGC TATCTCGAAT 
CTCAACTACA TAAGTGTGGA AACCGAGGAC GAAAACCTGC AGGCTTCCGT GGCCGAAAAG 
AAGGTGGAAT CTGAAAAGAA ACTGCCCGAG GCTGGCAATC AACCTGGCCC GGAAAAGACC 
ACCAACGAAT TGAGGATCAA CGAGCCACCC TACTATCAGT CCAAAGAGCA GAACCAGCAG 
CCAAAGGAAC TCAAGAACCA GAGCAAATCC CTGGACGAAG TGGATGCGGC GCCACTCTAT 
CAGACCGACA AATTCAGCCA AGAGTGCTCG CAACTTTTGG AAAACCAACT GGGCAATGAC 
TACGTACTGC AGCCCTCCAA TGTGCCGGTG GCCCAGGATG ATGGCAACTA TTTGTTCCAG 
CCATCGAATA GACCCGTGGA GGAGCCCCTC CGGTTAAATC GGTGCACCAA CAGACAGCCC 
GGATATGGAA CACTGAAGCG CAGTGCCAGC GACGCATCCG CCGCCAGTCA CACAAGATCC 
AATGCCGACG ATGAGCTGGC CGAGATTATG CACGATTTTA AGAACAACGT GCTCTCCATT 
CGGGACGTGG AAGTGCTGGT GGAACGCTGG AAGCACCGCA ACGATGTACA GCAGAGCTTC 
CGGGAGAAAC AGGATCAGAT CGACCAGCTG CGACGCGAGT ACGAGCGCAT CCAGGAGCAG 
GTGAAGTCTC ATCTGAAGCG CGAAACGCCT TTCGAGCGGA TCAAGAAGTT CTTCTTGCGA 
TCCAAGACAC CTGGGGGAAA CGACAGCCTG CTGGACGAGA CCAAGTCATC GATAGCCACA 
AGCTGTAGCT TCAAGTCCGC GGGGGGCGTG AGCTCCAGCG CAGGAGCTGG ACGTCCCATC 
AGTTCGCTGA GCCTACAGAG TGTGTCCAGC TCGAGTTCCT CCTCCGGCAG ACTAAGCACC 
GGCAGCAATT GCAGTGGTGC CTCGCTGGGA GATTCGGGTA CCCACTCGGA TCACGAGGAT 
CGTCGCTTCC CGCACTGCCG GATGATGGAC AACTACATGG TGCCACCGAC ACCGAGACCC 
GTCTTTACGC CCAGCTCAAC GCCCGGTGAT GAGCGTCATC AGATTGTATT CCCCTCGCCC 
ATGTCCAGTT GCCAGCGCTT GAACACACCC ACCAGTCCCA CGGGATCCGA GCACTACCAA 
ATGTTCCCCT CGAACATACC CGTGTACGGC AGCCAGCCAC TGGTTGGATC CCAGAGCAGC 
AACTACCTGA ACACCATCAT GGAGGCCAAG GAGCAGGCGG AGACACAGCA CTACCAAAAC 
CAGAACGGAG TCACCCTGCA GCCCATTAAG CTGAACGAGC GATCGAACAT CATCTACGGC 
AAGCTAACCA AGAGCAGCTC CGCCAGCGGA TGCAGCTCCT TCAAACCCAA GCAGATCCCA 
GTACCATGTC CCCAGGTGGG CAGCATCGAG GTCCAGGCCG AGGTCTACCA GAATGTGGGT 
GACGTTGCTC CGGAAAAGGA CTCCAAGGCA GAGGAGGAAA CGCCAAACTA CATGAACTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stumps ( NM_169589.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731924.1 Drosophila melanogaster stumps, transcript variant B (stumps), mRNA.

Price & Availability↑

CloneID	OFa04883
Clone ID Related Accession (Same CDS sequence)	NM_169589.2
Accession Version	NM_169589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stumps, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169589.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCTGA AAGTGGAAAA TATCGAAATG GAGACGTTCT GTTGCGACAA CTGCGGCAAC 
GTGAACAACA CGAGTGGATC GAGTGCATCC GGATTCCTGG GCTCTCCCGG CTACAACATG 
GACGACATCC TGATCATCAC CGCCAAGCAC AGCGAGCGCG CCTATTTGAG GGCCACTTTC 
CTGAAGAACC ACTTCGACAA GATCACCAAA CAGCGCGGAC GGAAACCATT CAATTTTCTG 
CACATCAAAA TTGACGATGG TCCCTTCAGC GAGGAGATTG CCCACAAGTA TCAGAACACA 
GCTTTGCAGA TTGTGATCCT CTGTCCCGCA CTTTTGGCCC TTCCTCACAG CTTTCTGATG 
ACCCAGCTAT CGGCCATTAT TCGCGTGGAA AAGGTACTGG CCATTTTGTT GGATGTCAGC 
GAGGAGAAGG TGAAGGAGAT GCACAAGTCG GCACTGCCCA GTTACTACAA GTGGCGTCGT 
TGTGTGGTGC GCGACAATGA CCAGGTGCAG ATCAGCAATA TCCTGGGCAT TGCCACCGAT 
ATCCTGGGAC GCGCGTTGTG CCAGCGACCA CCGTGCCAGG ATAGCTCTGG CGGAGGAGGT 
GGTGGTCTCT CGCGCAGCTT CTCTAGCTGC ACCGAGGGAT TCACCGTGCT GCCCAAGAAG 
GTCAAGCTGG GCCAAAACAA GGTGGTTGCT CTGCTGGCAG AACCGCTGGA GAAGAACGAC 
ACCCTGAAGC TGCAGGTGGA GAAGTCCGGT GAGCTAATCG AGCTGCGCAA CTTCAAGTGC 
CGCAATCCCT ACACCCTGCA GTTCAATATC CCAGAGGCCT GCATGGAGAT CTCCACCATG 
ATCGAGATTC GCATCGAGAA GAACAACAAG AGTCTGGGTG CCAGGCCCAT CAAGTGCGAG 
AGTCGTCTGC GGGAACTGGA GCAGCTGCTG CGTATTGAGG ACTCGCCAAT TGAGTTCATG 
TGCCATGCCC TGGGAATTGG ACCCGTGGAG CGGGATGCCC TCGATCAGCA CTTGCTGCAG 
TGCTTCCAAC GCAACATGCC ACCCAACTTC CACCTGCTGA GTGGTCCCAG CGAGCGTCAG 
CCACACTTCT TCACCCGTCT GGAATCCAGT CCAGAGGAGT ACCCCACATT GCTGCACTTC 
GCCGCCAGAT GGGGCCTGAA TCGCCTGTGC ATCCAGCTGA TGGAGTGCCC AGGTGGCGAC 
ACCGCATGCG GAGTAAGAAA CTGCGCCGGA CGCACTCCGG CCGAGTTGGC CGAACAGGAG 
GGGCATCACA AGTTGGCCCA GAACATCGTC AGTTTCGCAG AGTTTCACGA ACTGACCACC 
ATGTACCACT ACTTCAAGGG AGTGACTCCG CCAGGCGAGA GCTGTCCCAA GCCCAGTGCC 
AATGTGGTGA TTGAAGCACA TCCCGTTAAG GCCGGAAAGA CCAACAAGCC ACCGAAGGAA 
CTGCCCACGG CGGAGTATAT GGAGATGTCC AGTGGATCGG AGCGGGAGAA CACACCTGAA 
GTGAGGACCA AGACGGAAAC CCTTGCTATC TCGAATCTCA ACTACATAAG TGTGGAAACC 
GAGGACGAAA ACCTGCAGGC TTCCGTGGCC GAAAAGAAGG TGGAATCTGA AAAGAAACTG 
CCCGAGGCTG GCAATCAACC TGGCCCGGAA AAGACCACCA ACGAATTGAG GATCAACGAG 
CCACCCTACT ATCAGTCCAA AGAGCAGAAC CAGCAGCCAA AGGAACTCAA GAACCAGAGC 
AAATCCCTGG ACGAAGTGGA TGCGGCGCCA CTCTATCAGA CCGACAAATT CAGCCAAGAG 
TGCTCGCAAC TTTTGGAAAA CCAACTGGGC AATGACTACG TACTGCAGCC CTCCAATGTG 
CCGGTGGCCC AGGATGATGG CAACTATTTG TTCCAGCCAT CGAATAGACC CGTGGAGGAG 
CCCCTCCGGT TAAATCGGTG CACCAACAGA CAGCCCGGAT ATGGAACACT GAAGCGCAGT 
GCCAGCGACG CATCCGCCGC CAGTCACACA AGATCCAATG CCGACGATGA GCTGGCCGAG 
ATTATGCACG ATTTTAAGAA CAACGTGCTC TCCATTCGGG ACGTGGAAGT GCTGGTGGAA 
CGCTGGAAGC ACCGCAACGA TGTACAGCAG AGCTTCCGGG AGAAACAGGA TCAGATCGAC 
CAGCTGCGAC GCGAGTACGA GCGCATCCAG GAGCAGGTGA AGTCTCATCT GAAGCGCGAA 
ACGCCTTTCG AGCGGATCAA GAAGTTCTTC TTGCGATCCA AGACACCTGG GGGAAACGAC 
AGCCTGCTGG ACGAGACCAA GTCATCGATA GCCACAAGCT GTAGCTTCAA GTCCGCGGGG 
GGCGTGAGCT CCAGCGCAGG AGCTGGACGT CCCATCAGTT CGCTGAGCCT ACAGAGTGTG 
TCCAGCTCGA GTTCCTCCTC CGGCAGACTA AGCACCGGCA GCAATTGCAG TGGTGCCTCG 
CTGGGAGATT CGGGTACCCA CTCGGATCAC GAGGATCGTC GCTTCCCGCA CTGCCGGATG 
ATGGACAACT ACATGGTGCC ACCGACACCG AGACCCGTCT TTACGCCCAG CTCAACGCCC 
GGTGATGAGC GTCATCAGAT TGTATTCCCC TCGCCCATGT CCAGTTGCCA GCGCTTGAAC 
ACACCCACCA GTCCCACGGG ATCCGAGCAC TACCAAATGT TCCCCTCGAA CATACCCGTG 
TACGGCAGCC AGCCACTGGT TGGATCCCAG AGCAGCAACT ACCTGAACAC CATCATGGAG 
GCCAAGGAGC AGGCGGAGAC ACAGCACTAC CAAAACCAGA ACGGAGTCAC CCTGCAGCCC 
ATTAAGCTGA ACGAGCGATC GAACATCATC TACGGCAAGC TAACCAAGAG CAGCTCCGCC 
AGCGGATGCA GCTCCTTCAA ACCCAAGCAG ATCCCAGTAC CATGTCCCCA GGTGGGCAGC 
ATCGAGGTCC AGGCCGAGGT CTACCAGAAT GTGGGTGACG TTGCTCCGGA AAAGGACTCC 
AAGGCAGAGG AGGAAACGCC AAACTACATG AACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731924.1
CDS	168..3206
Translation	MLLKVENIEMETFCCDNCGNVNNTSGSSASGFLGSPGYNMDDILIITAKHSERAYLRATFLKNHFDKITKQRGRKPFNFLHIKIDDGPFSEEIAHKYQNTALQIVILCPALLALPHSFLMTQLSAIIRVEKVLAILLDVSEEKVKEMHKSALPSYYKWRRCVVRDNDQVQISNILGIATDILGRALCQRPPCQDSSGGGGGGLSRSFSSCTEGFTVLPKKVKLGQNKVVALLAEPLEKNDTLKLQVEKSGELIELRNFKCRNPYTLQFNIPEACMEISTMIEIRIEKNNKSLGARPIKCESRLRELEQLLRIEDSPIEFMCHALGIGPVERDALDQHLLQCFQRNMPPNFHLLSGPSERQPHFFTRLESSPEEYPTLLHFAARWGLNRLCIQLMECPGGDTACGVRNCAGRTPAELAEQEGHHKLAQNIVSFAEFHELTTMYHYFKGVTPPGESCPKPSANVVIEAHPVKAGKTNKPPKELPTAEYMEMSSGSERENTPEVRTKTETLAISNLNYISVETEDENLQASVAEKKVESEKKLPEAGNQPGPEKTTNELRINEPPYYQSKEQNQQPKELKNQSKSLDEVDAAPLYQTDKFSQECSQLLENQLGNDYVLQPSNVPVAQDDGNYLFQPSNRPVEEPLRLNRCTNRQPGYGTLKRSASDASAASHTRSNADDELAEIMHDFKNNVLSIRDVEVLVERWKHRNDVQQSFREKQDQIDQLRREYERIQEQVKSHLKRETPFERIKKFFLRSKTPGGNDSLLDETKSSIATSCSFKSAGGVSSSAGAGRPISSLSLQSVSSSSSSSGRLSTGSNCSGASLGDSGTHSDHEDRRFPHCRMMDNYMVPPTPRPVFTPSSTPGDERHQIVFPSPMSSCQRLNTPTSPTGSEHYQMFPSNIPVYGSQPLVGSQSSNYLNTIMEAKEQAETQHYQNQNGVTLQPIKLNERSNIIYGKLTKSSSASGCSSFKPKQIPVPCPQVGSIEVQAEVYQNVGDVAPEKDSKAEEETPNYMNC

Target ORF information:

RefSeq Version	NM_169589.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stumps, transcript variant B (stumps), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169589.2

ORF Insert Sequence:

ATGTTGCTGA AAGTGGAAAA TATCGAAATG GAGACGTTCT GTTGCGACAA CTGCGGCAAC 
GTGAACAACA CGAGTGGATC GAGTGCATCC GGATTCCTGG GCTCTCCCGG CTACAACATG 
GACGACATCC TGATCATCAC CGCCAAGCAC AGCGAGCGCG CCTATTTGAG GGCCACTTTC 
CTGAAGAACC ACTTCGACAA GATCACCAAA CAGCGCGGAC GGAAACCATT CAATTTTCTG 
CACATCAAAA TTGACGATGG TCCCTTCAGC GAGGAGATTG CCCACAAGTA TCAGAACACA 
GCTTTGCAGA TTGTGATCCT CTGTCCCGCA CTTTTGGCCC TTCCTCACAG CTTTCTGATG 
ACCCAGCTAT CGGCCATTAT TCGCGTGGAA AAGGTACTGG CCATTTTGTT GGATGTCAGC 
GAGGAGAAGG TGAAGGAGAT GCACAAGTCG GCACTGCCCA GTTACTACAA GTGGCGTCGT 
TGTGTGGTGC GCGACAATGA CCAGGTGCAG ATCAGCAATA TCCTGGGCAT TGCCACCGAT 
ATCCTGGGAC GCGCGTTGTG CCAGCGACCA CCGTGCCAGG ATAGCTCTGG CGGAGGAGGT 
GGTGGTCTCT CGCGCAGCTT CTCTAGCTGC ACCGAGGGAT TCACCGTGCT GCCCAAGAAG 
GTCAAGCTGG GCCAAAACAA GGTGGTTGCT CTGCTGGCAG AACCGCTGGA GAAGAACGAC 
ACCCTGAAGC TGCAGGTGGA GAAGTCCGGT GAGCTAATCG AGCTGCGCAA CTTCAAGTGC 
CGCAATCCCT ACACCCTGCA GTTCAATATC CCAGAGGCCT GCATGGAGAT CTCCACCATG 
ATCGAGATTC GCATCGAGAA GAACAACAAG AGTCTGGGTG CCAGGCCCAT CAAGTGCGAG 
AGTCGTCTGC GGGAACTGGA GCAGCTGCTG CGTATTGAGG ACTCGCCAAT TGAGTTCATG 
TGCCATGCCC TGGGAATTGG ACCCGTGGAG CGGGATGCCC TCGATCAGCA CTTGCTGCAG 
TGCTTCCAAC GCAACATGCC ACCCAACTTC CACCTGCTGA GTGGTCCCAG CGAGCGTCAG 
CCACACTTCT TCACCCGTCT GGAATCCAGT CCAGAGGAGT ACCCCACATT GCTGCACTTC 
GCCGCCAGAT GGGGCCTGAA TCGCCTGTGC ATCCAGCTGA TGGAGTGCCC AGGTGGCGAC 
ACCGCATGCG GAGTAAGAAA CTGCGCCGGA CGCACTCCGG CCGAGTTGGC CGAACAGGAG 
GGGCATCACA AGTTGGCCCA GAACATCGTC AGTTTCGCAG AGTTTCACGA ACTGACCACC 
ATGTACCACT ACTTCAAGGG AGTGACTCCG CCAGGCGAGA GCTGTCCCAA GCCCAGTGCC 
AATGTGGTGA TTGAAGCACA TCCCGTTAAG GCCGGAAAGA CCAACAAGCC ACCGAAGGAA 
CTGCCCACGG CGGAGTATAT GGAGATGTCC AGTGGATCGG AGCGGGAGAA CACACCTGAA 
GTGAGGACCA AGACGGAAAC CCTTGCTATC TCGAATCTCA ACTACATAAG TGTGGAAACC 
GAGGACGAAA ACCTGCAGGC TTCCGTGGCC GAAAAGAAGG TGGAATCTGA AAAGAAACTG 
CCCGAGGCTG GCAATCAACC TGGCCCGGAA AAGACCACCA ACGAATTGAG GATCAACGAG 
CCACCCTACT ATCAGTCCAA AGAGCAGAAC CAGCAGCCAA AGGAACTCAA GAACCAGAGC 
AAATCCCTGG ACGAAGTGGA TGCGGCGCCA CTCTATCAGA CCGACAAATT CAGCCAAGAG 
TGCTCGCAAC TTTTGGAAAA CCAACTGGGC AATGACTACG TACTGCAGCC CTCCAATGTG 
CCGGTGGCCC AGGATGATGG CAACTATTTG TTCCAGCCAT CGAATAGACC CGTGGAGGAG 
CCCCTCCGGT TAAATCGGTG CACCAACAGA CAGCCCGGAT ATGGAACACT GAAGCGCAGT 
GCCAGCGACG CATCCGCCGC CAGTCACACA AGATCCAATG CCGACGATGA GCTGGCCGAG 
ATTATGCACG ATTTTAAGAA CAACGTGCTC TCCATTCGGG ACGTGGAAGT GCTGGTGGAA 
CGCTGGAAGC ACCGCAACGA TGTACAGCAG AGCTTCCGGG AGAAACAGGA TCAGATCGAC 
CAGCTGCGAC GCGAGTACGA GCGCATCCAG GAGCAGGTGA AGTCTCATCT GAAGCGCGAA 
ACGCCTTTCG AGCGGATCAA GAAGTTCTTC TTGCGATCCA AGACACCTGG GGGAAACGAC 
AGCCTGCTGG ACGAGACCAA GTCATCGATA GCCACAAGCT GTAGCTTCAA GTCCGCGGGG 
GGCGTGAGCT CCAGCGCAGG AGCTGGACGT CCCATCAGTT CGCTGAGCCT ACAGAGTGTG 
TCCAGCTCGA GTTCCTCCTC CGGCAGACTA AGCACCGGCA GCAATTGCAG TGGTGCCTCG 
CTGGGAGATT CGGGTACCCA CTCGGATCAC GAGGATCGTC GCTTCCCGCA CTGCCGGATG 
ATGGACAACT ACATGGTGCC ACCGACACCG AGACCCGTCT TTACGCCCAG CTCAACGCCC 
GGTGATGAGC GTCATCAGAT TGTATTCCCC TCGCCCATGT CCAGTTGCCA GCGCTTGAAC 
ACACCCACCA GTCCCACGGG ATCCGAGCAC TACCAAATGT TCCCCTCGAA CATACCCGTG 
TACGGCAGCC AGCCACTGGT TGGATCCCAG AGCAGCAACT ACCTGAACAC CATCATGGAG 
GCCAAGGAGC AGGCGGAGAC ACAGCACTAC CAAAACCAGA ACGGAGTCAC CCTGCAGCCC 
ATTAAGCTGA ACGAGCGATC GAACATCATC TACGGCAAGC TAACCAAGAG CAGCTCCGCC 
AGCGGATGCA GCTCCTTCAA ACCCAAGCAG ATCCCAGTAC CATGTCCCCA GGTGGGCAGC 
ATCGAGGTCC AGGCCGAGGT CTACCAGAAT GTGGGTGACG TTGCTCCGGA AAAGGACTCC 
AAGGCAGAGG AGGAAACGCC AAACTACATG AACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stumps ( NM_001275642.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262571.1 Drosophila melanogaster stumps, transcript variant D (stumps), mRNA.

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CloneID	OFa07988
Clone ID Related Accession (Same CDS sequence)	NM_001275642.2
Accession Version	NM_001275642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stumps, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_001275642.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAACGA AGGGCAGTTG TCGCCTCTTT AGCTGCGTTT CGGGGCAGCG AAGGCCGTCG 
AAGGAGGAGG AGGAGGATTA TGTGGACATG GAGATGAACA ACTGCGGCAA CGTGAACAAC 
ACGAGTGGAT CGAGTGCATC CGGATTCCTG GGCTCTCCCG GCTACAACAT GGACGACATC 
CTGATCATCA CCGCCAAGCA CAGCGAGCGC GCCTATTTGA GGGCCACTTT CCTGAAGAAC 
CACTTCGACA AGATCACCAA ACAGCGCGGA CGGAAACCAT TCAATTTTCT GCACATCAAA 
ATTGACGATG GTCCCTTCAG CGAGGAGATT GCCCACAAGT ATCAGAACAC AGCTTTGCAG 
ATTGTGATCC TCTGTCCCGC ACTTTTGGCC CTTCCTCACA GCTTTCTGAT GACCCAGCTA 
TCGGCCATTA TTCGCGTGGA AAAGGTACTG GCCATTTTGT TGGATGTCAG CGAGGAGAAG 
GTGAAGGAGA TGCACAAGTC GGCACTGCCC AGTTACTACA AGTGGCGTCG TTGTGTGGTG 
CGCGACAATG ACCAGGTGCA GATCAGCAAT ATCCTGGGCA TTGCCACCGA TATCCTGGGA 
CGCGCGTTGT GCCAGCGACC ACCGTGCCAG GATAGCTCTG GCGGAGGAGG TGGTGGTCTC 
TCGCGCAGCT TCTCTAGCTG CACCGAGGGA TTCACCGTGC TGCCCAAGAA GGTCAAGCTG 
GGCCAAAACA AGGTGGTTGC TCTGCTGGCA GAACCGCTGG AGAAGAACGA CACCCTGAAG 
CTGCAGGTGG AGAAGTCCGG TGAGCTAATC GAGCTGCGCA ACTTCAAGTG CCGCAATCCC 
TACACCCTGC AGTTCAATAT CCCAGAGGCC TGCATGGAGA TCTCCACCAT GATCGAGATT 
CGCATCGAGA AGAACAACAA GAGTCTGGGT GCCAGGCCCA TCAAGTGCGA GAGTCGTCTG 
CGGGAACTGG AGCAGCTGCT GCGTATTGAG GACTCGCCAA TTGAGTTCAT GTGCCATGCC 
CTGGGAATTG GACCCGTGGA GCGGGATGCC CTCGATCAGC ACTTGCTGCA GTGCTTCCAA 
CGCAACATGC CACCCAACTT CCACCTGCTG AGTGGTCCCA GCGAGCGTCA GCCACACTTC 
TTCACCCGTC TGGAATCCAG TCCAGAGGAG TACCCCACAT TGCTGCACTT CGCCGCCAGA 
TGGGGCCTGA ATCGCCTGTG CATCCAGCTG ATGGAGTGCC CAGGTGGCGA CACCGCATGC 
GGAGTAAGAA ACTGCGCCGG ACGCACTCCG GCCGAGTTGG CCGAACAGGA GGGGCATCAC 
AAGTTGGCCC AGAACATCGT CAGTTTCGCA GAGTTTCACG AACTGACCAC CATGTACCAC 
TACTTCAAGG GAGTGACTCC GCCAGGCGAG AGCTGTCCCA AGCCCAGTGC CAATGTGGTG 
ATTGAAGCAC ATCCCGTTAA GGCCGGAAAG ACCAACAAGC CACCGAAGGA ACTGCCCACG 
GCGGAGTATA TGGAGATGTC CAGTGGATCG GAGCGGGAGA ACACACCTGA AGTGAGGACC 
AAGACGGAAA CCCTTGCTAT CTCGAATCTC AACTACATAA GTGTGGAAAC CGAGGACGAA 
AACCTGCAGG CTTCCGTGGC CGAAAAGAAG GTGGAATCTG AAAAGAAACT GCCCGAGGCT 
GGCAATCAAC CTGGCCCGGA AAAGACCACC AACGAATTGA GGATCAACGA GCCACCCTAC 
TATCAGTCCA AAGAGCAGAA CCAGCAGCCA AAGGAACTCA AGAACCAGAG CAAATCCCTG 
GACGAAGTGG ATGCGGCGCC ACTCTATCAG ACCGACAAAT TCAGCCAAGA GTGCTCGCAA 
CTTTTGGAAA ACCAACTGGG CAATGACTAC GTACTGCAGC CCTCCAATGT GCCGGTGGCC 
CAGGATGATG GCAACTATTT GTTCCAGCCA TCGAATAGAC CCGTGGAGGA GCCCCTCCGG 
TTAAATCGGT GCACCAACAG ACAGCCCGGA TATGGAACAC TGAAGCGCAG TGCCAGCGAC 
GCATCCGCCG CCAGTCACAC AAGATCCAAT GCCGACGATG AGCTGGCCGA GATTATGCAC 
GATTTTAAGA ACAACGTGCT CTCCATTCGG GACGTGGAAG TGCTGGTGGA ACGCTGGAAG 
CACCGCAACG ATGTACAGCA GAGCTTCCGG GAGAAACAGG ATCAGATCGA CCAGCTGCGA 
CGCGAGTACG AGCGCATCCA GGAGCAGGTG AAGTCTCATC TGAAGCGCGA AACGCCTTTC 
GAGCGGATCA AGAAGTTCTT CTTGCGATCC AAGACACCTG GGGGAAACGA CAGCCTGCTG 
GACGAGACCA AGTCATCGAT AGCCACAAGC TGTAGCTTCA AGTCCGCGGG GGGCGTGAGC 
TCCAGCGCAG GAGCTGGACG TCCCATCAGT TCGCTGAGCC TACAGAGTGT GTCCAGCTCG 
AGTTCCTCCT CCGGCAGACT AAGCACCGGC AGCAATTGCA GTGGTGCCTC GCTGGGAGAT 
TCGGGTACCC ACTCGGATCA CGAGGATCGT CGCTTCCCGC ACTGCCGGAT GATGGACAAC 
TACATGGTGC CACCGACACC GAGACCCGTC TTTACGCCCA GCTCAACGCC CGGTGATGAG 
CGTCATCAGA TTGTATTCCC CTCGCCCATG TCCAGTTGCC AGCGCTTGAA CACACCCACC 
AGTCCCACGG GATCCGAGCA CTACCAAATG TTCCCCTCGA ACATACCCGT GTACGGCAGC 
CAGCCACTGG TTGGATCCCA GAGCAGCAAC TACCTGAACA CCATCATGGA GGCCAAGGAG 
CAGGCGGAGA CACAGCACTA CCAAAACCAG AACGGAGTCA CCCTGCAGCC CATTAAGCTG 
AACGAGCGAT CGAACATCAT CTACGGCAAG CTAACCAAGA GCAGCTCCGC CAGCGGATGC 
AGCTCCTTCA AACCCAAGCA GATCCCAGTA CCATGTCCCC AGGTGGGCAG CATCGAGGTC 
CAGGCCGAGG TCTACCAGAA TGTGGGTGAC GTTGCTCCGG AAAAGGACTC CAAGGCAGAG 
GAGGAAACGC CAAACTACAT GAACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262571.1
CDS	152..3241
Translation	MPTKGSCRLFSCVSGQRRPSKEEEEDYVDMEMNNCGNVNNTSGSSASGFLGSPGYNMDDILIITAKHSERAYLRATFLKNHFDKITKQRGRKPFNFLHIKIDDGPFSEEIAHKYQNTALQIVILCPALLALPHSFLMTQLSAIIRVEKVLAILLDVSEEKVKEMHKSALPSYYKWRRCVVRDNDQVQISNILGIATDILGRALCQRPPCQDSSGGGGGGLSRSFSSCTEGFTVLPKKVKLGQNKVVALLAEPLEKNDTLKLQVEKSGELIELRNFKCRNPYTLQFNIPEACMEISTMIEIRIEKNNKSLGARPIKCESRLRELEQLLRIEDSPIEFMCHALGIGPVERDALDQHLLQCFQRNMPPNFHLLSGPSERQPHFFTRLESSPEEYPTLLHFAARWGLNRLCIQLMECPGGDTACGVRNCAGRTPAELAEQEGHHKLAQNIVSFAEFHELTTMYHYFKGVTPPGESCPKPSANVVIEAHPVKAGKTNKPPKELPTAEYMEMSSGSERENTPEVRTKTETLAISNLNYISVETEDENLQASVAEKKVESEKKLPEAGNQPGPEKTTNELRINEPPYYQSKEQNQQPKELKNQSKSLDEVDAAPLYQTDKFSQECSQLLENQLGNDYVLQPSNVPVAQDDGNYLFQPSNRPVEEPLRLNRCTNRQPGYGTLKRSASDASAASHTRSNADDELAEIMHDFKNNVLSIRDVEVLVERWKHRNDVQQSFREKQDQIDQLRREYERIQEQVKSHLKRETPFERIKKFFLRSKTPGGNDSLLDETKSSIATSCSFKSAGGVSSSAGAGRPISSLSLQSVSSSSSSSGRLSTGSNCSGASLGDSGTHSDHEDRRFPHCRMMDNYMVPPTPRPVFTPSSTPGDERHQIVFPSPMSSCQRLNTPTSPTGSEHYQMFPSNIPVYGSQPLVGSQSSNYLNTIMEAKEQAETQHYQNQNGVTLQPIKLNERSNIIYGKLTKSSSASGCSSFKPKQIPVPCPQVGSIEVQAEVYQNVGDVAPEKDSKAEEETPNYMNC

Target ORF information:

RefSeq Version	NM_001275642.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stumps, transcript variant D (stumps), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275642.2

ORF Insert Sequence:

ATGCCAACGA AGGGCAGTTG TCGCCTCTTT AGCTGCGTTT CGGGGCAGCG AAGGCCGTCG 
AAGGAGGAGG AGGAGGATTA TGTGGACATG GAGATGAACA ACTGCGGCAA CGTGAACAAC 
ACGAGTGGAT CGAGTGCATC CGGATTCCTG GGCTCTCCCG GCTACAACAT GGACGACATC 
CTGATCATCA CCGCCAAGCA CAGCGAGCGC GCCTATTTGA GGGCCACTTT CCTGAAGAAC 
CACTTCGACA AGATCACCAA ACAGCGCGGA CGGAAACCAT TCAATTTTCT GCACATCAAA 
ATTGACGATG GTCCCTTCAG CGAGGAGATT GCCCACAAGT ATCAGAACAC AGCTTTGCAG 
ATTGTGATCC TCTGTCCCGC ACTTTTGGCC CTTCCTCACA GCTTTCTGAT GACCCAGCTA 
TCGGCCATTA TTCGCGTGGA AAAGGTACTG GCCATTTTGT TGGATGTCAG CGAGGAGAAG 
GTGAAGGAGA TGCACAAGTC GGCACTGCCC AGTTACTACA AGTGGCGTCG TTGTGTGGTG 
CGCGACAATG ACCAGGTGCA GATCAGCAAT ATCCTGGGCA TTGCCACCGA TATCCTGGGA 
CGCGCGTTGT GCCAGCGACC ACCGTGCCAG GATAGCTCTG GCGGAGGAGG TGGTGGTCTC 
TCGCGCAGCT TCTCTAGCTG CACCGAGGGA TTCACCGTGC TGCCCAAGAA GGTCAAGCTG 
GGCCAAAACA AGGTGGTTGC TCTGCTGGCA GAACCGCTGG AGAAGAACGA CACCCTGAAG 
CTGCAGGTGG AGAAGTCCGG TGAGCTAATC GAGCTGCGCA ACTTCAAGTG CCGCAATCCC 
TACACCCTGC AGTTCAATAT CCCAGAGGCC TGCATGGAGA TCTCCACCAT GATCGAGATT 
CGCATCGAGA AGAACAACAA GAGTCTGGGT GCCAGGCCCA TCAAGTGCGA GAGTCGTCTG 
CGGGAACTGG AGCAGCTGCT GCGTATTGAG GACTCGCCAA TTGAGTTCAT GTGCCATGCC 
CTGGGAATTG GACCCGTGGA GCGGGATGCC CTCGATCAGC ACTTGCTGCA GTGCTTCCAA 
CGCAACATGC CACCCAACTT CCACCTGCTG AGTGGTCCCA GCGAGCGTCA GCCACACTTC 
TTCACCCGTC TGGAATCCAG TCCAGAGGAG TACCCCACAT TGCTGCACTT CGCCGCCAGA 
TGGGGCCTGA ATCGCCTGTG CATCCAGCTG ATGGAGTGCC CAGGTGGCGA CACCGCATGC 
GGAGTAAGAA ACTGCGCCGG ACGCACTCCG GCCGAGTTGG CCGAACAGGA GGGGCATCAC 
AAGTTGGCCC AGAACATCGT CAGTTTCGCA GAGTTTCACG AACTGACCAC CATGTACCAC 
TACTTCAAGG GAGTGACTCC GCCAGGCGAG AGCTGTCCCA AGCCCAGTGC CAATGTGGTG 
ATTGAAGCAC ATCCCGTTAA GGCCGGAAAG ACCAACAAGC CACCGAAGGA ACTGCCCACG 
GCGGAGTATA TGGAGATGTC CAGTGGATCG GAGCGGGAGA ACACACCTGA AGTGAGGACC 
AAGACGGAAA CCCTTGCTAT CTCGAATCTC AACTACATAA GTGTGGAAAC CGAGGACGAA 
AACCTGCAGG CTTCCGTGGC CGAAAAGAAG GTGGAATCTG AAAAGAAACT GCCCGAGGCT 
GGCAATCAAC CTGGCCCGGA AAAGACCACC AACGAATTGA GGATCAACGA GCCACCCTAC 
TATCAGTCCA AAGAGCAGAA CCAGCAGCCA AAGGAACTCA AGAACCAGAG CAAATCCCTG 
GACGAAGTGG ATGCGGCGCC ACTCTATCAG ACCGACAAAT TCAGCCAAGA GTGCTCGCAA 
CTTTTGGAAA ACCAACTGGG CAATGACTAC GTACTGCAGC CCTCCAATGT GCCGGTGGCC 
CAGGATGATG GCAACTATTT GTTCCAGCCA TCGAATAGAC CCGTGGAGGA GCCCCTCCGG 
TTAAATCGGT GCACCAACAG ACAGCCCGGA TATGGAACAC TGAAGCGCAG TGCCAGCGAC 
GCATCCGCCG CCAGTCACAC AAGATCCAAT GCCGACGATG AGCTGGCCGA GATTATGCAC 
GATTTTAAGA ACAACGTGCT CTCCATTCGG GACGTGGAAG TGCTGGTGGA ACGCTGGAAG 
CACCGCAACG ATGTACAGCA GAGCTTCCGG GAGAAACAGG ATCAGATCGA CCAGCTGCGA 
CGCGAGTACG AGCGCATCCA GGAGCAGGTG AAGTCTCATC TGAAGCGCGA AACGCCTTTC 
GAGCGGATCA AGAAGTTCTT CTTGCGATCC AAGACACCTG GGGGAAACGA CAGCCTGCTG 
GACGAGACCA AGTCATCGAT AGCCACAAGC TGTAGCTTCA AGTCCGCGGG GGGCGTGAGC 
TCCAGCGCAG GAGCTGGACG TCCCATCAGT TCGCTGAGCC TACAGAGTGT GTCCAGCTCG 
AGTTCCTCCT CCGGCAGACT AAGCACCGGC AGCAATTGCA GTGGTGCCTC GCTGGGAGAT 
TCGGGTACCC ACTCGGATCA CGAGGATCGT CGCTTCCCGC ACTGCCGGAT GATGGACAAC 
TACATGGTGC CACCGACACC GAGACCCGTC TTTACGCCCA GCTCAACGCC CGGTGATGAG 
CGTCATCAGA TTGTATTCCC CTCGCCCATG TCCAGTTGCC AGCGCTTGAA CACACCCACC 
AGTCCCACGG GATCCGAGCA CTACCAAATG TTCCCCTCGA ACATACCCGT GTACGGCAGC 
CAGCCACTGG TTGGATCCCA GAGCAGCAAC TACCTGAACA CCATCATGGA GGCCAAGGAG 
CAGGCGGAGA CACAGCACTA CCAAAACCAG AACGGAGTCA CCCTGCAGCC CATTAAGCTG 
AACGAGCGAT CGAACATCAT CTACGGCAAG CTAACCAAGA GCAGCTCCGC CAGCGGATGC 
AGCTCCTTCA AACCCAAGCA GATCCCAGTA CCATGTCCCC AGGTGGGCAG CATCGAGGTC 
CAGGCCGAGG TCTACCAGAA TGTGGGTGAC GTTGCTCCGG AAAAGGACTC CAAGGCAGAG 
GAGGAAACGC CAAACTACAT GAACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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stumps cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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