HtrA2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	HtrA2
Entrez Gene ID	41756
Full Name	HTRA2-related serine protease
Synonyms	CG8464,DmHtrA2,Dmel\CG8464,HtrA2/Omi,Omi,dOmi,domi,htrA2,htrA2/omi,omi
General protein information	Preferred Names HTRA2-related serine protease Names CG8464 gene product from transcript CG8464-RB CG8464-PA CG8464-PB HtrA2-PA HtrA2-PB serine protease HTRA2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88C3-88C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275636.1	NP_001262565.1	HTRA2-related serine protease, isoform B
NM_142109.2	NP_650366.1	HTRA2-related serine protease, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	HTRA2	NP_037379.1
Pan troglodytes (chimpanzee)	HTRA2	XP_003309140.2
Canis lupus familiaris (dog)	HTRA2	XP_532992.3
Danio rerio (zebrafish)	LOC560917	XP_002663637.2
Danio rerio (zebrafish)	zgc:173425	NP_001098612.1
Danio rerio (zebrafish)	si:busm1-sl7.7	XP_003199743.1
Danio rerio (zebrafish)	LOC100003308	NP_001103205.1
Danio rerio (zebrafish)	LOC799006	XP_001339411.5
Danio rerio (zebrafish)	si:dkey-84o3.3	XP_002665896.3
Bos taurus (cattle)	HTRA2	NP_001071424.1
Gallus gallus (chicken)	HTRA2	XP_423666.3
Danio rerio (zebrafish)	LOC100536969	XP_003199752.2
Danio rerio (zebrafish)	si:dkey-84o3.7	XP_001335201.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000240	XP_310886.5
Danio rerio (zebrafish)	zgc:174193	NP_001108178.1
Danio rerio (zebrafish)	si:dkey-33c12.10	XP_003199749.2
Danio rerio (zebrafish)	si:dkey-33c12.2	XP_005172803.1
Danio rerio (zebrafish)	LOC100329860	XP_002664149.3
Danio rerio (zebrafish)	LOC797876	XP_001923767.4
Danio rerio (zebrafish)	LOC100331049	XP_002665901.2
Danio rerio (zebrafish)	LOC799537	NP_001103640.1
Rattus norvegicus (Norway rat)	Htra2	NP_001100069.1
Danio rerio (zebrafish)	LOC100149563	NP_001122292.1
Danio rerio (zebrafish)	LOC797799	NP_001103998.1
Drosophila melanogaster (fruit fly)	HtrA2	NP_001262565.1
	HTRA2	XP_001110803.1
Mus musculus (house mouse)	Htra2	NP_062726.3
Danio rerio (zebrafish)	LOC793110	XP_001332804.5
Danio rerio (zebrafish)	LOC793521	XP_001333305.1
Danio rerio (zebrafish)	LOC100330919	XP_005161042.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mitochondrial serine protease HtrA2/Omi is an important mediator of germ cell death.
Title: Alternative germ cell death pathway in Drosophila involves HtrA2/Omi, lysosomes, and a caspase-9 counterpart.

Data show the presence of apoptosis at the cellular level in both ade2 and Prat mutants, and the upregulated gene HtrA2, which encodes an apoptosis effector and is thus a candidate for initiating apoptosis in response to purine depletion.
Title: A link between impaired purine nucleotide synthesis and apoptosis in Drosophila melanogaster.

HtrA2 was shown to be phosphorylated in a PINK1-dependent manner, suggesting it might act in the PINK1 pathway.
Title: Drosophila HtrA2 is dispensable for apoptosis but acts downstream of PINK1 independently from Parkin.

found that Rhomboid-7, a mitochondrial protease not previously implicated in PD, acts as an upstream component of this pathway, and showed that it is required to cleave the precursor forms of both Pink1 and Omi
Title: Rhomboid-7 and HtrA2/Omi act in a common pathway with the Parkinson's disease factors Pink1 and Parkin.

The binding of DIAP1 to dOmi resulted in DIAP1-mediated polyubiquitination of dOmi, suggesting that DIAP1 could target dOmi for proteasomal degradation.
Title: The interaction of DIAP1 with dOmi/HtrA2 regulates cell death in Drosophila.

The following HtrA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HtrA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04868	NM_001275636.1 Latest version!	Drosophila melanogaster HTRA2-related serine protease (HtrA2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04868	NM_142109.2 Latest version!	Drosophila melanogaster HTRA2-related serine protease (HtrA2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HtrA2 ( NM_001275636.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262565.1 Drosophila melanogaster HTRA2-related serine protease (HtrA2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04868
Clone ID Related Accession (Same CDS sequence)	NM_001275636.1 , NM_142109.2
Accession Version	NM_001275636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HTRA2-related serine protease, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTTGC GCGGTTCCCA CCGCTTGGAA GTGATCTTCA AACGCTGCAT TGCCTCCCCG 
GTGTTACACA GTCATGCAGC CAATCGGAGG AGCAGTCAAC TAGCGATCAA AGAAGGCGAT 
CCAAACAGCA ATGGTAACAG TGGGCAATAT CAGCAAAATG GTGAACAAAA AGAAAAAGGC 
TGGAGACGAT TGGTTCGATT CTTTGTGCCC TTCTCCCTGG GCGCTGTGGT CAGTGCGGCG 
ATCATACAGC GGGAAGACTT AACGCCAACG ATCGCCGCTT CCAAAATGAC CGGTCGTCGG 
CGGGACTTCA ACTTTATAGC TGATGTGGTG GCAGGTTGTG CGGATTCGGT GGTCTACATT 
GAGATTAAGG ACACCCGCCA CTTTGACTAC TTCAGCGGCC AACCGATTAC GGCATCGAAT 
GGTTCCGGCT TCATTATTGA GCAGAACGGT CTCATCCTTA CCAACGCCCA TGTGGTGATC 
AACAAACCAC ACACAATGGT ACAGGTGAGG CTATCCGATG GCAGGACTTT TCCCGCCACC 
ATCGAGGACG TGGATCAGAC ATCCGATCTG GCGACTTTGC GCATACAGGT GAACAATCTT 
TCGGTGATGC GGCTAGGCAA GAGCAGTACC CTGCGATCCG GCGAGTGGGT TGTGGCCTTA 
GGCAGTCCGC TGGCCCTAAG CAATACGGTG ACAGCAGGCG TAATCAGTTC AACGCAGCGT 
GCATCTCAAG AACTTGGCCT TCGAAACAGG GATATCAACT ACCTGCAAAC GGATGCTGCG 
ATTACGTTTG GCAACTCCGG CGGTCCCTTG GTCAATCTGG ATGGCGAGGC TATTGGAGTT 
AACTCGATGA AGGTGACGGC TGGCATTAGC TTCGCAATAC CCATTGACTA TGTAAAGGTG 
TTTCTGGAGC GGGCGGCCGA GAAACGAAAG AAGGGCTCGG CCTACAAGAC TGGCTATCCG 
GTGAAGCGGT ACATGGGCAT CACCATGCTG ACGCTCACAC CGGATATTCT GTTCGAGCTC 
AAGTCACGAA GCCAAAACAT GCCCAGCAAC TTAACGCACG GCGTGCTCGT GTGGAAGGTT 
ATCGTTGGGT CACCGGCGCA CAGTGGTGGA CTCCAACCCG GAGACATAGT GACCCACATA 
AACAAGAAGG AGATCAAGAA CTCATCGGAT GTCTACGATG CCCTGGCGGA TAATAGTAAA 
ACTCTAGATA TAGTGATCCT GCGAGGTGTG AAGCAGATGC ACGTGACCAT TACGCCAGAA 
GATCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262565.1
CDS	307..1575
Translation	MALRGSHRLEVIFKRCIASPVLHSHAANRRSSQLAIKEGDPNSNGNSGQYQQNGEQKEKGWRRLVRFFVPFSLGAVVSAAIIQREDLTPTIAASKMTGRRRDFNFIADVVAGCADSVVYIEIKDTRHFDYFSGQPITASNGSGFIIEQNGLILTNAHVVINKPHTMVQVRLSDGRTFPATIEDVDQTSDLATLRIQVNNLSVMRLGKSSTLRSGEWVVALGSPLALSNTVTAGVISSTQRASQELGLRNRDINYLQTDAAITFGNSGGPLVNLDGEAIGVNSMKVTAGISFAIPIDYVKVFLERAAEKRKKGSAYKTGYPVKRYMGITMLTLTPDILFELKSRSQNMPSNLTHGVLVWKVIVGSPAHSGGLQPGDIVTHINKKEIKNSSDVYDALADNSKTLDIVILRGVKQMHVTITPEDP

Target ORF information:

RefSeq Version	NM_001275636.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HTRA2-related serine protease (HtrA2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275636.1

ORF Insert Sequence:

ATGGCTTTGC GCGGTTCCCA CCGCTTGGAA GTGATCTTCA AACGCTGCAT TGCCTCCCCG 
GTGTTACACA GTCATGCAGC CAATCGGAGG AGCAGTCAAC TAGCGATCAA AGAAGGCGAT 
CCAAACAGCA ATGGTAACAG TGGGCAATAT CAGCAAAATG GTGAACAAAA AGAAAAAGGC 
TGGAGACGAT TGGTTCGATT CTTTGTGCCC TTCTCCCTGG GCGCTGTGGT CAGTGCGGCG 
ATCATACAGC GGGAAGACTT AACGCCAACG ATCGCCGCTT CCAAAATGAC CGGTCGTCGG 
CGGGACTTCA ACTTTATAGC TGATGTGGTG GCAGGTTGTG CGGATTCGGT GGTCTACATT 
GAGATTAAGG ACACCCGCCA CTTTGACTAC TTCAGCGGCC AACCGATTAC GGCATCGAAT 
GGTTCCGGCT TCATTATTGA GCAGAACGGT CTCATCCTTA CCAACGCCCA TGTGGTGATC 
AACAAACCAC ACACAATGGT ACAGGTGAGG CTATCCGATG GCAGGACTTT TCCCGCCACC 
ATCGAGGACG TGGATCAGAC ATCCGATCTG GCGACTTTGC GCATACAGGT GAACAATCTT 
TCGGTGATGC GGCTAGGCAA GAGCAGTACC CTGCGATCCG GCGAGTGGGT TGTGGCCTTA 
GGCAGTCCGC TGGCCCTAAG CAATACGGTG ACAGCAGGCG TAATCAGTTC AACGCAGCGT 
GCATCTCAAG AACTTGGCCT TCGAAACAGG GATATCAACT ACCTGCAAAC GGATGCTGCG 
ATTACGTTTG GCAACTCCGG CGGTCCCTTG GTCAATCTGG ATGGCGAGGC TATTGGAGTT 
AACTCGATGA AGGTGACGGC TGGCATTAGC TTCGCAATAC CCATTGACTA TGTAAAGGTG 
TTTCTGGAGC GGGCGGCCGA GAAACGAAAG AAGGGCTCGG CCTACAAGAC TGGCTATCCG 
GTGAAGCGGT ACATGGGCAT CACCATGCTG ACGCTCACAC CGGATATTCT GTTCGAGCTC 
AAGTCACGAA GCCAAAACAT GCCCAGCAAC TTAACGCACG GCGTGCTCGT GTGGAAGGTT 
ATCGTTGGGT CACCGGCGCA CAGTGGTGGA CTCCAACCCG GAGACATAGT GACCCACATA 
AACAAGAAGG AGATCAAGAA CTCATCGGAT GTCTACGATG CCCTGGCGGA TAATAGTAAA 
ACTCTAGATA TAGTGATCCT GCGAGGTGTG AAGCAGATGC ACGTGACCAT TACGCCAGAA 
GATCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HtrA2 ( NM_142109.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650366.1 Drosophila melanogaster HTRA2-related serine protease (HtrA2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04868
Clone ID Related Accession (Same CDS sequence)	NM_001275636.1 , NM_142109.2
Accession Version	NM_142109.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HTRA2-related serine protease, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142109.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTTGC GCGGTTCCCA CCGCTTGGAA GTGATCTTCA AACGCTGCAT TGCCTCCCCG 
GTGTTACACA GTCATGCAGC CAATCGGAGG AGCAGTCAAC TAGCGATCAA AGAAGGCGAT 
CCAAACAGCA ATGGTAACAG TGGGCAATAT CAGCAAAATG GTGAACAAAA AGAAAAAGGC 
TGGAGACGAT TGGTTCGATT CTTTGTGCCC TTCTCCCTGG GCGCTGTGGT CAGTGCGGCG 
ATCATACAGC GGGAAGACTT AACGCCAACG ATCGCCGCTT CCAAAATGAC CGGTCGTCGG 
CGGGACTTCA ACTTTATAGC TGATGTGGTG GCAGGTTGTG CGGATTCGGT GGTCTACATT 
GAGATTAAGG ACACCCGCCA CTTTGACTAC TTCAGCGGCC AACCGATTAC GGCATCGAAT 
GGTTCCGGCT TCATTATTGA GCAGAACGGT CTCATCCTTA CCAACGCCCA TGTGGTGATC 
AACAAACCAC ACACAATGGT ACAGGTGAGG CTATCCGATG GCAGGACTTT TCCCGCCACC 
ATCGAGGACG TGGATCAGAC ATCCGATCTG GCGACTTTGC GCATACAGGT GAACAATCTT 
TCGGTGATGC GGCTAGGCAA GAGCAGTACC CTGCGATCCG GCGAGTGGGT TGTGGCCTTA 
GGCAGTCCGC TGGCCCTAAG CAATACGGTG ACAGCAGGCG TAATCAGTTC AACGCAGCGT 
GCATCTCAAG AACTTGGCCT TCGAAACAGG GATATCAACT ACCTGCAAAC GGATGCTGCG 
ATTACGTTTG GCAACTCCGG CGGTCCCTTG GTCAATCTGG ATGGCGAGGC TATTGGAGTT 
AACTCGATGA AGGTGACGGC TGGCATTAGC TTCGCAATAC CCATTGACTA TGTAAAGGTG 
TTTCTGGAGC GGGCGGCCGA GAAACGAAAG AAGGGCTCGG CCTACAAGAC TGGCTATCCG 
GTGAAGCGGT ACATGGGCAT CACCATGCTG ACGCTCACAC CGGATATTCT GTTCGAGCTC 
AAGTCACGAA GCCAAAACAT GCCCAGCAAC TTAACGCACG GCGTGCTCGT GTGGAAGGTT 
ATCGTTGGGT CACCGGCGCA CAGTGGTGGA CTCCAACCCG GAGACATAGT GACCCACATA 
AACAAGAAGG AGATCAAGAA CTCATCGGAT GTCTACGATG CCCTGGCGGA TAATAGTAAA 
ACTCTAGATA TAGTGATCCT GCGAGGTGTG AAGCAGATGC ACGTGACCAT TACGCCAGAA 
GATCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650366.1
CDS	55..1323
Translation	MALRGSHRLEVIFKRCIASPVLHSHAANRRSSQLAIKEGDPNSNGNSGQYQQNGEQKEKGWRRLVRFFVPFSLGAVVSAAIIQREDLTPTIAASKMTGRRRDFNFIADVVAGCADSVVYIEIKDTRHFDYFSGQPITASNGSGFIIEQNGLILTNAHVVINKPHTMVQVRLSDGRTFPATIEDVDQTSDLATLRIQVNNLSVMRLGKSSTLRSGEWVVALGSPLALSNTVTAGVISSTQRASQELGLRNRDINYLQTDAAITFGNSGGPLVNLDGEAIGVNSMKVTAGISFAIPIDYVKVFLERAAEKRKKGSAYKTGYPVKRYMGITMLTLTPDILFELKSRSQNMPSNLTHGVLVWKVIVGSPAHSGGLQPGDIVTHINKKEIKNSSDVYDALADNSKTLDIVILRGVKQMHVTITPEDP

Target ORF information:

RefSeq Version	NM_142109.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HTRA2-related serine protease (HtrA2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142109.2

ORF Insert Sequence:

ATGGCTTTGC GCGGTTCCCA CCGCTTGGAA GTGATCTTCA AACGCTGCAT TGCCTCCCCG 
GTGTTACACA GTCATGCAGC CAATCGGAGG AGCAGTCAAC TAGCGATCAA AGAAGGCGAT 
CCAAACAGCA ATGGTAACAG TGGGCAATAT CAGCAAAATG GTGAACAAAA AGAAAAAGGC 
TGGAGACGAT TGGTTCGATT CTTTGTGCCC TTCTCCCTGG GCGCTGTGGT CAGTGCGGCG 
ATCATACAGC GGGAAGACTT AACGCCAACG ATCGCCGCTT CCAAAATGAC CGGTCGTCGG 
CGGGACTTCA ACTTTATAGC TGATGTGGTG GCAGGTTGTG CGGATTCGGT GGTCTACATT 
GAGATTAAGG ACACCCGCCA CTTTGACTAC TTCAGCGGCC AACCGATTAC GGCATCGAAT 
GGTTCCGGCT TCATTATTGA GCAGAACGGT CTCATCCTTA CCAACGCCCA TGTGGTGATC 
AACAAACCAC ACACAATGGT ACAGGTGAGG CTATCCGATG GCAGGACTTT TCCCGCCACC 
ATCGAGGACG TGGATCAGAC ATCCGATCTG GCGACTTTGC GCATACAGGT GAACAATCTT 
TCGGTGATGC GGCTAGGCAA GAGCAGTACC CTGCGATCCG GCGAGTGGGT TGTGGCCTTA 
GGCAGTCCGC TGGCCCTAAG CAATACGGTG ACAGCAGGCG TAATCAGTTC AACGCAGCGT 
GCATCTCAAG AACTTGGCCT TCGAAACAGG GATATCAACT ACCTGCAAAC GGATGCTGCG 
ATTACGTTTG GCAACTCCGG CGGTCCCTTG GTCAATCTGG ATGGCGAGGC TATTGGAGTT 
AACTCGATGA AGGTGACGGC TGGCATTAGC TTCGCAATAC CCATTGACTA TGTAAAGGTG 
TTTCTGGAGC GGGCGGCCGA GAAACGAAAG AAGGGCTCGG CCTACAAGAC TGGCTATCCG 
GTGAAGCGGT ACATGGGCAT CACCATGCTG ACGCTCACAC CGGATATTCT GTTCGAGCTC 
AAGTCACGAA GCCAAAACAT GCCCAGCAAC TTAACGCACG GCGTGCTCGT GTGGAAGGTT 
ATCGTTGGGT CACCGGCGCA CAGTGGTGGA CTCCAACCCG GAGACATAGT GACCCACATA 
AACAAGAAGG AGATCAAGAA CTCATCGGAT GTCTACGATG CCCTGGCGGA TAATAGTAAA 
ACTCTAGATA TAGTGATCCT GCGAGGTGTG AAGCAGATGC ACGTGACCAT TACGCCAGAA 
GATCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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HtrA2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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