RpII15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RpII15
Entrez Gene ID	41741
Full Name	RNA polymerase II 15kD subunit
Synonyms	CG3284,Dmel\CG3284,H5,Pol II,PolII,RNA Pol II,RNA pol II,RNA polII,RNAP,RNAP II,RNApolII,RPII15,RpII215,Rpb9,dRPB9,l(3)88Be,l(3)Z23,pol II,polII
General protein information	Preferred Names RNA polymerase II 15kD subunit Names CG3284 gene product from transcript CG3284-RC CG3284-PA CG3284-PB CG3284-PC RNA polymerase II RNA polymerase II 15kd subunit RpII15-PA RpII15-PB RpII15-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88B3-88B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260170.2	NP_001247099.1	RNA polymerase II 15kD subunit, isoform B
NM_169575.3	NP_731898.1	RNA polymerase II 15kD subunit, isoform A
NM_001300397.1	NP_001287326.1	RNA polymerase II 15kD subunit, isoform C

Pathways from BioSystems

Homologies

Bos taurus (cattle)	POLR2I	NP_001069797.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008393	XP_317057.2
Gallus gallus (chicken)	POLR2I	XP_423952.3
Danio rerio (zebrafish)	polr2i	NP_001006013.2
Drosophila melanogaster (fruit fly)	RpII15	NP_001247099.1
Saccharomyces cerevisiae (baker's yeast)	RPB9	NP_011445.1
Schizosaccharomyces pombe (fission yeast)	rpb9	NP_594705.1
Oryza sativa (rice)	Os12g0560400	NP_001067017.1
Pan troglodytes (chimpanzee)	POLR2I	XP_001162834.1
Mus musculus (house mouse)	Polr2i	NP_081535.1
Caenorhabditis elegans (roundworm)	rpb-9	NP_505062.1
Kluyveromyces lactis	KLLA0D03234g	XP_453210.1
Xenopus tropicalis (tropical clawed frog)	polr2i	XP_002932661.1
Homo sapiens (human)	POLR2I	NP_006224.1
Canis lupus familiaris (dog)	POLR2I	XP_853642.1
Eremothecium gossypii	AGOS_AFL088W	NP_985460.1
	POLR2I	XP_001102318.1
Magnaporthe oryzae (rice blast fungus)	MGG_07391	XP_003711269.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RpII15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RpII15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04858	NM_001260170.2 Latest version!	Drosophila melanogaster RNA polymerase II 15kD subunit (RpII15), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa04858	NM_001300397.1 Latest version!	Drosophila melanogaster RNA polymerase II 15kD subunit (RpII15), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa04858	NM_169575.3 Latest version!	Drosophila melanogaster RNA polymerase II 15kD subunit (RpII15), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RpII15 ( NM_001260170.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247099.1 Drosophila melanogaster RNA polymerase II 15kD subunit (RpII15), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04858
Clone ID Related Accession (Same CDS sequence)	NM_001260170.2 , NM_169575.3 , NM_001300397.1
Accession Version	NM_001260170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RNA polymerase II 15kD subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260170.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACTG CCTTTGATGC CGCACACACT GAGGGGCCGG GATTCGTGGG CATTCGGTTC 
TGCCAGGAGT GCAACAACAT GCTGTACCCC AAGGAGGACA AGGAGAACAA GATCCTGCTG 
TACGCCTGCC GGAATTGCGA TTACAAACAG GAGGCGGACT CCAACTGCAT CTACGTGAAC 
AAGATTATGC ACGAGATCGA CGAGCTGACC CACATTGTGC CCGACGTGAT TTCCGATCCC 
ACGCTGCCGC GCACCGAAGA CCACGCTTGT CCCAAGTGCT CCCATCGGGA GGCGGTCTTC 
TTCCAGGCGC AAACTCGTCG CGCCGAAGAG GAGATGCGAC TGTACTACGT GTGCACCAAC 
CAGAACTGCA CCCACCGTTG GACGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247099.1
CDS	37..426
Translation	MTTAFDAAHTEGPGFVGIRFCQECNNMLYPKEDKENKILLYACRNCDYKQEADSNCIYVNKIMHEIDELTHIVPDVISDPTLPRTEDHACPKCSHREAVFFQAQTRRAEEEMRLYYVCTNQNCTHRWTE

Target ORF information:

RefSeq Version	NM_001260170.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RNA polymerase II 15kD subunit (RpII15), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260170.2

ORF Insert Sequence:

ATGACGACTG CCTTTGATGC CGCACACACT GAGGGGCCGG GATTCGTGGG CATTCGGTTC 
TGCCAGGAGT GCAACAACAT GCTGTACCCC AAGGAGGACA AGGAGAACAA GATCCTGCTG 
TACGCCTGCC GGAATTGCGA TTACAAACAG GAGGCGGACT CCAACTGCAT CTACGTGAAC 
AAGATTATGC ACGAGATCGA CGAGCTGACC CACATTGTGC CCGACGTGAT TTCCGATCCC 
ACGCTGCCGC GCACCGAAGA CCACGCTTGT CCCAAGTGCT CCCATCGGGA GGCGGTCTTC 
TTCCAGGCGC AAACTCGTCG CGCCGAAGAG GAGATGCGAC TGTACTACGT GTGCACCAAC 
CAGAACTGCA CCCACCGTTG GACGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RpII15 ( NM_169575.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731898.1 Drosophila melanogaster RNA polymerase II 15kD subunit (RpII15), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04858
Clone ID Related Accession (Same CDS sequence)	NM_001260170.2 , NM_169575.3 , NM_001300397.1
Accession Version	NM_169575.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RNA polymerase II 15kD subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169575.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACTG CCTTTGATGC CGCACACACT GAGGGGCCGG GATTCGTGGG CATTCGGTTC 
TGCCAGGAGT GCAACAACAT GCTGTACCCC AAGGAGGACA AGGAGAACAA GATCCTGCTG 
TACGCCTGCC GGAATTGCGA TTACAAACAG GAGGCGGACT CCAACTGCAT CTACGTGAAC 
AAGATTATGC ACGAGATCGA CGAGCTGACC CACATTGTGC CCGACGTGAT TTCCGATCCC 
ACGCTGCCGC GCACCGAAGA CCACGCTTGT CCCAAGTGCT CCCATCGGGA GGCGGTCTTC 
TTCCAGGCGC AAACTCGTCG CGCCGAAGAG GAGATGCGAC TGTACTACGT GTGCACCAAC 
CAGAACTGCA CCCACCGTTG GACGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731898.1
CDS	44..433
Translation	MTTAFDAAHTEGPGFVGIRFCQECNNMLYPKEDKENKILLYACRNCDYKQEADSNCIYVNKIMHEIDELTHIVPDVISDPTLPRTEDHACPKCSHREAVFFQAQTRRAEEEMRLYYVCTNQNCTHRWTE

Target ORF information:

RefSeq Version	NM_169575.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RNA polymerase II 15kD subunit (RpII15), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169575.3

ORF Insert Sequence:

ATGACGACTG CCTTTGATGC CGCACACACT GAGGGGCCGG GATTCGTGGG CATTCGGTTC 
TGCCAGGAGT GCAACAACAT GCTGTACCCC AAGGAGGACA AGGAGAACAA GATCCTGCTG 
TACGCCTGCC GGAATTGCGA TTACAAACAG GAGGCGGACT CCAACTGCAT CTACGTGAAC 
AAGATTATGC ACGAGATCGA CGAGCTGACC CACATTGTGC CCGACGTGAT TTCCGATCCC 
ACGCTGCCGC GCACCGAAGA CCACGCTTGT CCCAAGTGCT CCCATCGGGA GGCGGTCTTC 
TTCCAGGCGC AAACTCGTCG CGCCGAAGAG GAGATGCGAC TGTACTACGT GTGCACCAAC 
CAGAACTGCA CCCACCGTTG GACGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RpII15 ( NM_001300397.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287326.1 Drosophila melanogaster RNA polymerase II 15kD subunit (RpII15), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04858
Clone ID Related Accession (Same CDS sequence)	NM_001260170.2 , NM_169575.3 , NM_001300397.1
Accession Version	NM_001300397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RNA polymerase II 15kD subunit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACTG CCTTTGATGC CGCACACACT GAGGGGCCGG GATTCGTGGG CATTCGGTTC 
TGCCAGGAGT GCAACAACAT GCTGTACCCC AAGGAGGACA AGGAGAACAA GATCCTGCTG 
TACGCCTGCC GGAATTGCGA TTACAAACAG GAGGCGGACT CCAACTGCAT CTACGTGAAC 
AAGATTATGC ACGAGATCGA CGAGCTGACC CACATTGTGC CCGACGTGAT TTCCGATCCC 
ACGCTGCCGC GCACCGAAGA CCACGCTTGT CCCAAGTGCT CCCATCGGGA GGCGGTCTTC 
TTCCAGGCGC AAACTCGTCG CGCCGAAGAG GAGATGCGAC TGTACTACGT GTGCACCAAC 
CAGAACTGCA CCCACCGTTG GACGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287326.1
CDS	44..433
Translation	MTTAFDAAHTEGPGFVGIRFCQECNNMLYPKEDKENKILLYACRNCDYKQEADSNCIYVNKIMHEIDELTHIVPDVISDPTLPRTEDHACPKCSHREAVFFQAQTRRAEEEMRLYYVCTNQNCTHRWTE

Target ORF information:

RefSeq Version	NM_001300397.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RNA polymerase II 15kD subunit (RpII15), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300397.1

ORF Insert Sequence:

ATGACGACTG CCTTTGATGC CGCACACACT GAGGGGCCGG GATTCGTGGG CATTCGGTTC 
TGCCAGGAGT GCAACAACAT GCTGTACCCC AAGGAGGACA AGGAGAACAA GATCCTGCTG 
TACGCCTGCC GGAATTGCGA TTACAAACAG GAGGCGGACT CCAACTGCAT CTACGTGAAC 
AAGATTATGC ACGAGATCGA CGAGCTGACC CACATTGTGC CCGACGTGAT TTCCGATCCC 
ACGCTGCCGC GCACCGAAGA CCACGCTTGT CCCAAGTGCT CCCATCGGGA GGCGGTCTTC 
TTCCAGGCGC AAACTCGTCG CGCCGAAGAG GAGATGCGAC TGTACTACGT GTGCACCAAC 
CAGAACTGCA CCCACCGTTG GACGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RpII15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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