ninaB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ninaB
Entrez Gene ID	41678
Full Name	neither inactivation nor afterpotential B
Synonyms	CG9347,DRPE65,Dmel\CG9347,RPE65,anon-WO0148162.1,anon-WO0148163.1,beta-diox
General protein information	Preferred Names neither inactivation nor afterpotential B Names CG9347 gene product from transcript CG9347-RA CG9347-PA beta-carotene dioxygenase ninaB-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87F11-87F11\|3-53.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_142050.3	NP_650307.2	neither inactivation nor afterpotential B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	ninaB	NP_650307.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008143	XP_317319.4
	BCO2	XP_001100991.2
Homo sapiens (human)	BCO2	NP_114144.4
Mus musculus (house mouse)	Bco2	NP_573480.1
Danio rerio (zebrafish)	LOC100330576	XP_002662060.3
Danio rerio (zebrafish)	bco2a	NP_001035402.1
Xenopus tropicalis (tropical clawed frog)	LOC100487224	XP_002942801.2
Pan troglodytes (chimpanzee)	BCO2	XP_001147647.1
Gallus gallus (chicken)	BCO2	XP_417929.2
Canis lupus familiaris (dog)	BCO2	XP_536572.3
Bos taurus (cattle)	BCO2	NP_001095457.2
Rattus norvegicus (Norway rat)	Bco2	NP_001121184.1
Danio rerio (zebrafish)	bco2l	NP_571874.1
Oryza sativa (rice)	Os01g0566500	NP_001043363.1
Xenopus tropicalis (tropical clawed frog)	bco2	NP_001006739.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study identifies NinaB as a key component for visual pigment production and provides evidence that chromophore in opsin-deficient photoreceptors can elicit retinal degeneration
Title: NinaB is essential for Drosophila vision but induces retinal degeneration in opsin-deficient photoreceptors.

Data show that carotenoid-isomerooxygenase activity of NinaB is more generally found in insects, and provide physiological evidence that carotenoids such as 11-cis-retinal can promote visual pigment biogenesis in the dark.
Title: NinaB combines carotenoid oxygenase and retinoid isomerase activity in a single polypeptide.

Beta-carotene is transported to the adult brain, where cellular uptake by NinaD allows cleavage by the NinaB enzyme to produce retinal
Title: Cellular sites of Drosophila NinaB and NinaD activity in vitamin A metabolism.

The following ninaB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ninaB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08355	NM_142050.3 Latest version!	Drosophila melanogaster neither inactivation nor afterpotential B (ninaB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ninaB ( NM_142050.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650307.2 Drosophila melanogaster neither inactivation nor afterpotential B (ninaB), mRNA.

Price & Availability↑

CloneID	OFa08355
Clone ID Related Accession (Same CDS sequence)	NM_142050.3
Accession Version	NM_142050.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neither inactivation nor afterpotential B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142050.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCCG GTGTCTTCAA GAGTTTTATG CGCGACTTCT TTGCGGTGAA ATACGATGAG 
CAGCGAAATG ATCCGCAAGC GGAACGACTG GATGGCAACG GACGACTGTA TCCCAACTGC 
TCGTCGGATG TGTGGCTGCG ATCCTGCGAG CGGGAGATAG TTGATCCCAT TGAGGGCCAT 
CACAGCGGGC ACATTCCCAA ATGGATATGC GGTAGTCTGT TGCGCAATGG ACCCGGCAGC 
TGGAAGGTGG GCGACATGAC CTTCGGCCAT CTGTTCGACT GCTCCGCCCT GCTGCACCGA 
TTTGCCATTC GGAATGGACG CGTCACCTAC CAGAATCGCT TCGTGGACAC GGAAACACTG 
CGAAAGAATC GCTCTGCCCA GCGGATTGTG GTCACGGAGT TTGGCACAGC TGCTGTTCCG 
GATCCCTGTC ACTCGATCTT CGATAGATTT GCGGCCATTT TTCGACCGGA TAGTGGAACG 
GATAACTCGA TGATTTCCAT ATATCCTTTC GGGGATCAGT ATTACACATT TACGGAGACG 
CCTTTTATGC ATAGAATAAA TCCCTGCACT TTGGCCACCG AAGCACGAAT CTGCACCACC 
GACTTCGTGG GCGTGGTGAA CCACACATCG CATCCGCATG TTCTTCCCAG TGGCACTGTC 
TACAACCTGG GCACCACAAT GACCAGATCT GGACCGGCAT ACACTATACT CAGTTTCCCG 
CACGGCGAGC AGATGTTCGA GGATGCTCAT GTGGTGGCCA CACTGCCGTG CCGCTGGAAA 
CTGCATCCCG GTTATATGCA CACCTTCGGC TTAACGGATC ACTACTTTGT GATTGTGGAG 
CAGCCGTTGT CCGTTTCGCT TACGGAGTAT ATCAAAGCCC AGCTAGGTGG ACAGAATTTA 
TCGGCGTGTC TCAAGTGGTT CGAGGATCGA CCGACACTAT TTCACCTTAT AGATCGGGTT 
TCCGGCAAAC TGGTGCAGAC CTACGAATCG GAAGCCTTCT TCTACCTGCA CATCATCAAC 
TGCTTTGAAC GGGATGGCCA CGTGGTGGTG GACATTTGCA GCTACAGGAA TCCCGAGATG 
ATCAACTGCA TGTATCTGGA GGCCATTGCC AATATGCAAA CGAATCCCAA TTATGCTACC 
CTCTTTCGTG GACGTCCCTT GAGATTCGTC CTGCCCTTGG GCACAATTCC TCCGGCAAGC 
ATCGCCAAGC GGGGACTGGT CAAGTCCTTC TCCCTTGCTG GACTAAGTGC TCCGCAGGTT 
TCTCGCACCA TGAAGCACTC GGTCTCGCAA TATGCGGATA TAACCTACAT GCCCACCAAT 
GGAAAGCAAG CCACTGCTGG AGAGGAAAGC CCCAAGCGAG ATGCCAAACG TGGCCGCTAT 
GAGGAGGAGA ATCTTGTCAA TCTGGTTACC ATGGAGGGCA GTCAAGCGGA GGCGTTTCAG 
GGCACCAATG GCATCCAACT GCGTCCGGAA ATGCTGTGTG ATTGGGGCTG TGAAACACCT 
AGGATCTATT ATGAACGGTA TATGGGCAAG AACTACCGAT ACTTCTACGC GATTAGCTCC 
GATGTGGATG CAGTGAATCC GGGCACCCTC ATCAAGGTGG ATGTGTGGAA TAAGAGCTGT 
CTAACCTGGT GCGAGGAGAA TGTCTATCCC AGTGAGCCCA TTTTTGTGCC TTCGCCGGAT 
CCGAAATCCG AGGACGATGG CGTTATCCTG GCCTCCATGG TGCTGGGCGG TCTCAACGAT 
CGCTATGTGG GCCTAATTGT GCTATGTGCC AAAACGATGA CCGAGCTGGG CCGTTGTGAT 
TTCCATACCA ATGGACCCGT GCCCAAGTGT CTCCATGGAT GGTTTGCACC CAATGCCATT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650307.2
CDS	137..1999
Translation	MAAGVFKSFMRDFFAVKYDEQRNDPQAERLDGNGRLYPNCSSDVWLRSCEREIVDPIEGHHSGHIPKWICGSLLRNGPGSWKVGDMTFGHLFDCSALLHRFAIRNGRVTYQNRFVDTETLRKNRSAQRIVVTEFGTAAVPDPCHSIFDRFAAIFRPDSGTDNSMISIYPFGDQYYTFTETPFMHRINPCTLATEARICTTDFVGVVNHTSHPHVLPSGTVYNLGTTMTRSGPAYTILSFPHGEQMFEDAHVVATLPCRWKLHPGYMHTFGLTDHYFVIVEQPLSVSLTEYIKAQLGGQNLSACLKWFEDRPTLFHLIDRVSGKLVQTYESEAFFYLHIINCFERDGHVVVDICSYRNPEMINCMYLEAIANMQTNPNYATLFRGRPLRFVLPLGTIPPASIAKRGLVKSFSLAGLSAPQVSRTMKHSVSQYADITYMPTNGKQATAGEESPKRDAKRGRYEEENLVNLVTMEGSQAEAFQGTNGIQLRPEMLCDWGCETPRIYYERYMGKNYRYFYAISSDVDAVNPGTLIKVDVWNKSCLTWCEENVYPSEPIFVPSPDPKSEDDGVILASMVLGGLNDRYVGLIVLCAKTMTELGRCDFHTNGPVPKCLHGWFAPNAI

Target ORF information:

RefSeq Version	NM_142050.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neither inactivation nor afterpotential B (ninaB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142050.3

ORF Insert Sequence:

ATGGCAGCCG GTGTCTTCAA GAGTTTTATG CGCGACTTCT TTGCGGTGAA ATACGATGAG 
CAGCGAAATG ATCCGCAAGC GGAACGACTG GATGGCAACG GACGACTGTA TCCCAACTGC 
TCGTCGGATG TGTGGCTGCG ATCCTGCGAG CGGGAGATAG TTGATCCCAT TGAGGGCCAT 
CACAGCGGGC ACATTCCCAA ATGGATATGC GGTAGTCTGT TGCGCAATGG ACCCGGCAGC 
TGGAAGGTGG GCGACATGAC CTTCGGCCAT CTGTTCGACT GCTCCGCCCT GCTGCACCGA 
TTTGCCATTC GGAATGGACG CGTCACCTAC CAGAATCGCT TCGTGGACAC GGAAACACTG 
CGAAAGAATC GCTCTGCCCA GCGGATTGTG GTCACGGAGT TTGGCACAGC TGCTGTTCCG 
GATCCCTGTC ACTCGATCTT CGATAGATTT GCGGCCATTT TTCGACCGGA TAGTGGAACG 
GATAACTCGA TGATTTCCAT ATATCCTTTC GGGGATCAGT ATTACACATT TACGGAGACG 
CCTTTTATGC ATAGAATAAA TCCCTGCACT TTGGCCACCG AAGCACGAAT CTGCACCACC 
GACTTCGTGG GCGTGGTGAA CCACACATCG CATCCGCATG TTCTTCCCAG TGGCACTGTC 
TACAACCTGG GCACCACAAT GACCAGATCT GGACCGGCAT ACACTATACT CAGTTTCCCG 
CACGGCGAGC AGATGTTCGA GGATGCTCAT GTGGTGGCCA CACTGCCGTG CCGCTGGAAA 
CTGCATCCCG GTTATATGCA CACCTTCGGC TTAACGGATC ACTACTTTGT GATTGTGGAG 
CAGCCGTTGT CCGTTTCGCT TACGGAGTAT ATCAAAGCCC AGCTAGGTGG ACAGAATTTA 
TCGGCGTGTC TCAAGTGGTT CGAGGATCGA CCGACACTAT TTCACCTTAT AGATCGGGTT 
TCCGGCAAAC TGGTGCAGAC CTACGAATCG GAAGCCTTCT TCTACCTGCA CATCATCAAC 
TGCTTTGAAC GGGATGGCCA CGTGGTGGTG GACATTTGCA GCTACAGGAA TCCCGAGATG 
ATCAACTGCA TGTATCTGGA GGCCATTGCC AATATGCAAA CGAATCCCAA TTATGCTACC 
CTCTTTCGTG GACGTCCCTT GAGATTCGTC CTGCCCTTGG GCACAATTCC TCCGGCAAGC 
ATCGCCAAGC GGGGACTGGT CAAGTCCTTC TCCCTTGCTG GACTAAGTGC TCCGCAGGTT 
TCTCGCACCA TGAAGCACTC GGTCTCGCAA TATGCGGATA TAACCTACAT GCCCACCAAT 
GGAAAGCAAG CCACTGCTGG AGAGGAAAGC CCCAAGCGAG ATGCCAAACG TGGCCGCTAT 
GAGGAGGAGA ATCTTGTCAA TCTGGTTACC ATGGAGGGCA GTCAAGCGGA GGCGTTTCAG 
GGCACCAATG GCATCCAACT GCGTCCGGAA ATGCTGTGTG ATTGGGGCTG TGAAACACCT 
AGGATCTATT ATGAACGGTA TATGGGCAAG AACTACCGAT ACTTCTACGC GATTAGCTCC 
GATGTGGATG CAGTGAATCC GGGCACCCTC ATCAAGGTGG ATGTGTGGAA TAAGAGCTGT 
CTAACCTGGT GCGAGGAGAA TGTCTATCCC AGTGAGCCCA TTTTTGTGCC TTCGCCGGAT 
CCGAAATCCG AGGACGATGG CGTTATCCTG GCCTCCATGG TGCTGGGCGG TCTCAACGAT 
CGCTATGTGG GCCTAATTGT GCTATGTGCC AAAACGATGA CCGAGCTGGG CCGTTGTGAT 
TTCCATACCA ATGGACCCGT GCCCAAGTGT CTCCATGGAT GGTTTGCACC CAATGCCATT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ninaB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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