flfl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	flfl
Entrez Gene ID	41675
Full Name	falafel
Synonyms	CG9351,Dmel\CG9351,Flfl,PP4R3,Ppp4R3,l(3)L4179
General protein information	Preferred Names falafel Names CG9351 gene product from transcript CG9351-RD CG9351-PB CG9351-PD CG9351-PE flfl-PB flfl-PD flfl-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87F10-87F11

mRNA and Protein(s)

mRNA	Protein	Name
NM_169549.2	NP_731850.2	falafel, isoform E
NM_169548.3	NP_731849.2	falafel, isoform B
NM_142047.4	NP_650304.2	falafel, isoform D

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMEK2	XP_003309065.1
Bos taurus (cattle)	SMEK2	NP_001178079.1
Gallus gallus (chicken)	SMEK2	XP_424305.3
Drosophila melanogaster (fruit fly)	flfl	NP_731849.2
Canis lupus familiaris (dog)	SMEK2	XP_005626173.1
	SMEK2	XP_001112386.2
Rattus norvegicus (Norway rat)	Smek2	NP_001101837.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003402	XP_311690.5
Homo sapiens (human)	SMEK2	NP_001116436.2
Mus musculus (house mouse)	Smek2	NP_598795.2
Danio rerio (zebrafish)	smek2	NP_956295.2
Xenopus tropicalis (tropical clawed frog)	smek2	NP_001005004.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The binding of Flfl to CENP-C is required to bring PP4 activity to centromeres to maintain CENP-C and attached core kinetochore proteins at chromosomes during mitosis.
Title: Centromeric binding and activity of Protein Phosphatase 4.

These data report for the first time an essential physiological function of flfl in maintaining tissue homeostasis and organ development
Title: Loss of flfl Triggers JNK-Dependent Cell Death in Drosophila.

identified the evolutionarily conserved Protein Phosphatase 4 (PP4) regulatory subunit PP4R3/Falafel (Flfl) as a key mediator specific for the localization of Miranda (Mira) and associated cell fate determinants during both interphase and mitosis
Title: Protein phosphatase 4 mediates localization of the Miranda complex during Drosophila neuroblast asymmetric divisions.

The following flfl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the flfl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08347	NM_142047.4 Latest version!	Drosophila melanogaster falafel (flfl), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08349	NM_169549.2 Latest version!	Drosophila melanogaster falafel (flfl), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08349	NM_169548.3 Latest version!	Drosophila melanogaster falafel (flfl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

flfl ( NM_142047.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650304.2 Drosophila melanogaster falafel (flfl), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa08347
Clone ID Related Accession (Same CDS sequence)	NM_142047.4
Accession Version	NM_142047.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	falafel, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142047.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACTG ACACCCGCCG ACGCGTCAAG CTGTACGCGC TGAATGCGGA ACGGCAATGG 
GATGATCGTG GCACTGGCCA CGTGTCATCC ACCTATGTGG AAAGACTAAA AGGCATCTCG 
CTTTTGGTGC GAGCCGAATC CGATGGATCT CTACTTTTGG AGAGCAAGAT ACAACCGGAC 
ACCGCATACC AGAAGCAACA GGATACGCTG ATCGTGTGGT CCGAGGGGGA TAACTTTGAT 
CTGGCCCTGA GTTTCCAGGA AAAGGCGGGC TGCGACGAAA TATGGGAAAA GATATGTCAG 
GTGCAAGGCA AGGATCCATC TGTAGAAATA ACTCAAGACA TCGTCGAAGA GTCTGAGGAT 
GAGCGGTTTG AGGACATGTC GGATACTGCG CCACCCATTG AGCTACCACC CTGCGAGCTT 
TCCCGGCTGG AGGACATCTC GGAAACGATA CAAAGCTGTC TCTCCACGCC GCTGCGAAAG 
GAAAAGTTGT CGATGGCTCT GGAGTCGGAG AGCTATATCA AGAAGCTGCT GAACTTGTTC 
CACGTGTGTG AGGACCTGGA CAATACCGAG GGCCTGCATC ACCTTTTTGA GATCTTCAAG 
AACATCTTCT TGCTAAATAA GAACGCGCTC TTTGAGATTA TGTTTGCGGA TGATACCATC 
TTTGATGTGG TCGGGTGCCT GGAGTACGAT CCGAGTGTGT CTCAGCCCAA AAAGCATCGA 
CAGTATCTGA AGCAGTTAGC CAAGTTCCGC GAGGCAGTGC CGATTAAAAA CCTGGACCTG 
CTGGCTAAGA TTCACCAGAC GTTCCGGGTG CAGTACATCC AGGACATTAT CCTGCCCACA 
CCATCGGTCT TTGTCGAAGA CAACATGCTG AACACCCTGT CCAGCTTCAT CTTCTTCAAC 
AAGGTGGAGA TTGTCACGAT GATCCAGGAC GACGAGCGGT ATCTCCTGGA TGTGTTTGCT 
GTTCTCACCG ATCCCACCAC CGGAGACGCG AAACGCCGCG ACACAGTTCT CTTCCTCAAG 
GAGTTCTGCA ACTATGCCCA GAACCTGCAG CCGCAGGGCA AGGACTCGTT CTACAAAACT 
CTCACCTGCC TAGGCATTCT GCAGGCGCTG GAACTCACTC TCGTGATGAA CGACAAGAAG 
ACCAAATCGG CCTCCATCGA CATCCTCACG GCCATCGTAG AGTTCTCGCC TCTGGTAGTG 
CGCAATTACA CGCTCAACCA GGCCAATCGG CCAGAAGTGG AGCGTATGCT ACTGAACATT 
GCCATCGAAC AAATGCTGAA TGACTCGGAG CCGGAGCTTG GAATTGCGGT GCAGCTAATG 
GGTATCGTCA AGATTCTGTT GGAGCCGGAA AACATGCTCA CCGAGAAGGG CGATTTTCTC 
AACTTCTTTT ACAAATACAG TGTGCAAACG TTAGTGGCCC CGGTGATACT TAACACGATA 
GGCGACCGTC CGCAAAACGA GGACTATCAG ACGGCCCAGC TGCTGGGAAT CGTCCTGGAC 
ATTCTTTCGT TCTGCGTGGA ACACCACAGT TATCACATTA AAAACTTTTT GTTGCAAAAG 
GATCTCCTCA AGCGAATACT GGTGCTAATG AAGAGCACAC ATACGTTCCT TGTCCTCGGC 
GCTCTGCGAT TACTGCGCAA GATAATTGCA CTTAAAGATG AGTTTTACAA TAGACACATT 
GTCAAGTGCA ATCTGTTTGC GCCGGTGGTA GATGCCTTCA TTCGCAACAA CGGCCGATAC 
AACCTTCTGG AGTCAGCAAT TCTGGAGCTG TTTGAGTTTA TAAAACTCGA GGATATCCGC 
ACTCTGTGCG TTTACTTCGT GGAGAACTTC AGCAAGATAT TTGATGAAAT CGAATACGTG 
CAGACATTTA AGTACTTGAA GAATCGCTAC GATCAGTACC AGGATCGGCT TAAGGACCGC 
GACAAGATGG AAAACCGAAC TGACGGCGGT GGCCGGTTTC GTCGGGATCA GCGGCAAATG 
GAGGAGGAAG AGGAAATGTG GTTTAACGAG GAGGACGATT TCACCGAGGA AATCGACACA 
TACAACAATG TCATGAAATC CGTCAGCGAA AAGAATGGCC CGCAAACGCA AAACCAGCAG 
AAATCCTCTC CGCCACACAG CACGTCGCCG CACAGTGGAT TGTTGGGAAG CCTGAGCACC 
ACCGCGTCAT CCACTGCCAC ATCCGCCACG TCAGGTGCAC CGGTGGCCAG CGGCAGCAGT 
AGCCCCGAGG CCATCTCCGC CGACGAGCAG ACGCAGGCGG CGGTGCATTT GGCTGCCGCT 
GCCCTTCAGC ACCACCAGCA ACAGCAACAG CAGCAGCAGC AGAACCCATT CCAGCAACAG 
ACACAGCCCG AGATCGCGGA GCTACAACAG CAGCTGAGCA GCGTGGAAGC GCCTCAAAGC 
CAGGAGTTGG AGCTGTCACA ATCGGCCGCC GCCAGTGCAT CGCCCACTTC GTCATCCTCG 
TCTCTGGAAG CCTCGACGTC CTCTTCATCG GCGTCATCGT CTTCATCCTC GTCGTCCTCG 
TCCTCGCCGC CCGGCTCATC GGCCGCAGCA TCGCTATGCG ATTCGGCGAC AGTAGCCGCC 
GTGGCAGCAT CACAGTTTCT CTCAACGATC GCCACAGCCA TGGCGGCCTC TGTGACGGCG 
GCGGCGGCAA CAAACAGCTC CCCGAGCATT TCGCCAGCTC CAGCTGTGTC CAGTCCGGAT 
ATCGAGAACG CCGACGCCCA GTTGCCGCCC AGCGATGACG CCAGCAGCCC GGCCAGTGGG 
GAGCAAGACG CTAACAGCAC GGAAGGTACC AGCAGTGAGG CCGACAAGAC GACGGCCAAA 
AAGGGTCTGG TGGACTATGA GAGTGATTCT GGCGAAGATG ACTACGAGGA GGATGAGTAC 
TCCGAGGGGC CACAAGCACA AAAGCGCGCG CGTCAGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650304.2
CDS	525..3446
Translation	MTTDTRRRVKLYALNAERQWDDRGTGHVSSTYVERLKGISLLVRAESDGSLLLESKIQPDTAYQKQQDTLIVWSEGDNFDLALSFQEKAGCDEIWEKICQVQGKDPSVEITQDIVEESEDERFEDMSDTAPPIELPPCELSRLEDISETIQSCLSTPLRKEKLSMALESESYIKKLLNLFHVCEDLDNTEGLHHLFEIFKNIFLLNKNALFEIMFADDTIFDVVGCLEYDPSVSQPKKHRQYLKQLAKFREAVPIKNLDLLAKIHQTFRVQYIQDIILPTPSVFVEDNMLNTLSSFIFFNKVEIVTMIQDDERYLLDVFAVLTDPTTGDAKRRDTVLFLKEFCNYAQNLQPQGKDSFYKTLTCLGILQALELTLVMNDKKTKSASIDILTAIVEFSPLVVRNYTLNQANRPEVERMLLNIAIEQMLNDSEPELGIAVQLMGIVKILLEPENMLTEKGDFLNFFYKYSVQTLVAPVILNTIGDRPQNEDYQTAQLLGIVLDILSFCVEHHSYHIKNFLLQKDLLKRILVLMKSTHTFLVLGALRLLRKIIALKDEFYNRHIVKCNLFAPVVDAFIRNNGRYNLLESAILELFEFIKLEDIRTLCVYFVENFSKIFDEIEYVQTFKYLKNRYDQYQDRLKDRDKMENRTDGGGRFRRDQRQMEEEEEMWFNEEDDFTEEIDTYNNVMKSVSEKNGPQTQNQQKSSPPHSTSPHSGLLGSLSTTASSTATSATSGAPVASGSSSPEAISADEQTQAAVHLAAAALQHHQQQQQQQQQNPFQQQTQPEIAELQQQLSSVEAPQSQELELSQSAAASASPTSSSSSLEASTSSSSASSSSSSSSSSSPPGSSAAASLCDSATVAAVAASQFLSTIATAMAASVTAAAATNSSPSISPAPAVSSPDIENADAQLPPSDDASSPASGEQDANSTEGTSSEADKTTAKKGLVDYESDSGEDDYEEDEYSEGPQAQKRARQA

Target ORF information:

RefSeq Version	NM_142047.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster falafel (flfl), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142047.4

ORF Insert Sequence:

ATGACGACTG ACACCCGCCG ACGCGTCAAG CTGTACGCGC TGAATGCGGA ACGGCAATGG 
GATGATCGTG GCACTGGCCA CGTGTCATCC ACCTATGTGG AAAGACTAAA AGGCATCTCG 
CTTTTGGTGC GAGCCGAATC CGATGGATCT CTACTTTTGG AGAGCAAGAT ACAACCGGAC 
ACCGCATACC AGAAGCAACA GGATACGCTG ATCGTGTGGT CCGAGGGGGA TAACTTTGAT 
CTGGCCCTGA GTTTCCAGGA AAAGGCGGGC TGCGACGAAA TATGGGAAAA GATATGTCAG 
GTGCAAGGCA AGGATCCATC TGTAGAAATA ACTCAAGACA TCGTCGAAGA GTCTGAGGAT 
GAGCGGTTTG AGGACATGTC GGATACTGCG CCACCCATTG AGCTACCACC CTGCGAGCTT 
TCCCGGCTGG AGGACATCTC GGAAACGATA CAAAGCTGTC TCTCCACGCC GCTGCGAAAG 
GAAAAGTTGT CGATGGCTCT GGAGTCGGAG AGCTATATCA AGAAGCTGCT GAACTTGTTC 
CACGTGTGTG AGGACCTGGA CAATACCGAG GGCCTGCATC ACCTTTTTGA GATCTTCAAG 
AACATCTTCT TGCTAAATAA GAACGCGCTC TTTGAGATTA TGTTTGCGGA TGATACCATC 
TTTGATGTGG TCGGGTGCCT GGAGTACGAT CCGAGTGTGT CTCAGCCCAA AAAGCATCGA 
CAGTATCTGA AGCAGTTAGC CAAGTTCCGC GAGGCAGTGC CGATTAAAAA CCTGGACCTG 
CTGGCTAAGA TTCACCAGAC GTTCCGGGTG CAGTACATCC AGGACATTAT CCTGCCCACA 
CCATCGGTCT TTGTCGAAGA CAACATGCTG AACACCCTGT CCAGCTTCAT CTTCTTCAAC 
AAGGTGGAGA TTGTCACGAT GATCCAGGAC GACGAGCGGT ATCTCCTGGA TGTGTTTGCT 
GTTCTCACCG ATCCCACCAC CGGAGACGCG AAACGCCGCG ACACAGTTCT CTTCCTCAAG 
GAGTTCTGCA ACTATGCCCA GAACCTGCAG CCGCAGGGCA AGGACTCGTT CTACAAAACT 
CTCACCTGCC TAGGCATTCT GCAGGCGCTG GAACTCACTC TCGTGATGAA CGACAAGAAG 
ACCAAATCGG CCTCCATCGA CATCCTCACG GCCATCGTAG AGTTCTCGCC TCTGGTAGTG 
CGCAATTACA CGCTCAACCA GGCCAATCGG CCAGAAGTGG AGCGTATGCT ACTGAACATT 
GCCATCGAAC AAATGCTGAA TGACTCGGAG CCGGAGCTTG GAATTGCGGT GCAGCTAATG 
GGTATCGTCA AGATTCTGTT GGAGCCGGAA AACATGCTCA CCGAGAAGGG CGATTTTCTC 
AACTTCTTTT ACAAATACAG TGTGCAAACG TTAGTGGCCC CGGTGATACT TAACACGATA 
GGCGACCGTC CGCAAAACGA GGACTATCAG ACGGCCCAGC TGCTGGGAAT CGTCCTGGAC 
ATTCTTTCGT TCTGCGTGGA ACACCACAGT TATCACATTA AAAACTTTTT GTTGCAAAAG 
GATCTCCTCA AGCGAATACT GGTGCTAATG AAGAGCACAC ATACGTTCCT TGTCCTCGGC 
GCTCTGCGAT TACTGCGCAA GATAATTGCA CTTAAAGATG AGTTTTACAA TAGACACATT 
GTCAAGTGCA ATCTGTTTGC GCCGGTGGTA GATGCCTTCA TTCGCAACAA CGGCCGATAC 
AACCTTCTGG AGTCAGCAAT TCTGGAGCTG TTTGAGTTTA TAAAACTCGA GGATATCCGC 
ACTCTGTGCG TTTACTTCGT GGAGAACTTC AGCAAGATAT TTGATGAAAT CGAATACGTG 
CAGACATTTA AGTACTTGAA GAATCGCTAC GATCAGTACC AGGATCGGCT TAAGGACCGC 
GACAAGATGG AAAACCGAAC TGACGGCGGT GGCCGGTTTC GTCGGGATCA GCGGCAAATG 
GAGGAGGAAG AGGAAATGTG GTTTAACGAG GAGGACGATT TCACCGAGGA AATCGACACA 
TACAACAATG TCATGAAATC CGTCAGCGAA AAGAATGGCC CGCAAACGCA AAACCAGCAG 
AAATCCTCTC CGCCACACAG CACGTCGCCG CACAGTGGAT TGTTGGGAAG CCTGAGCACC 
ACCGCGTCAT CCACTGCCAC ATCCGCCACG TCAGGTGCAC CGGTGGCCAG CGGCAGCAGT 
AGCCCCGAGG CCATCTCCGC CGACGAGCAG ACGCAGGCGG CGGTGCATTT GGCTGCCGCT 
GCCCTTCAGC ACCACCAGCA ACAGCAACAG CAGCAGCAGC AGAACCCATT CCAGCAACAG 
ACACAGCCCG AGATCGCGGA GCTACAACAG CAGCTGAGCA GCGTGGAAGC GCCTCAAAGC 
CAGGAGTTGG AGCTGTCACA ATCGGCCGCC GCCAGTGCAT CGCCCACTTC GTCATCCTCG 
TCTCTGGAAG CCTCGACGTC CTCTTCATCG GCGTCATCGT CTTCATCCTC GTCGTCCTCG 
TCCTCGCCGC CCGGCTCATC GGCCGCAGCA TCGCTATGCG ATTCGGCGAC AGTAGCCGCC 
GTGGCAGCAT CACAGTTTCT CTCAACGATC GCCACAGCCA TGGCGGCCTC TGTGACGGCG 
GCGGCGGCAA CAAACAGCTC CCCGAGCATT TCGCCAGCTC CAGCTGTGTC CAGTCCGGAT 
ATCGAGAACG CCGACGCCCA GTTGCCGCCC AGCGATGACG CCAGCAGCCC GGCCAGTGGG 
GAGCAAGACG CTAACAGCAC GGAAGGTACC AGCAGTGAGG CCGACAAGAC GACGGCCAAA 
AAGGGTCTGG TGGACTATGA GAGTGATTCT GGCGAAGATG ACTACGAGGA GGATGAGTAC 
TCCGAGGGGC CACAAGCACA AAAGCGCGCG CGTCAGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flfl ( NM_169549.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731850.2 Drosophila melanogaster falafel (flfl), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa08349
Clone ID Related Accession (Same CDS sequence)	NM_169549.2 , NM_169548.3
Accession Version	NM_169549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	falafel, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169549.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACTG ACACCCGCCG ACGCGTCAAG CTGTACGCGC TGAATGCGGA ACGGCAATGG 
GATGATCGTG GCACTGGCCA CGTGTCATCC ACCTATGTGG AAAGACTAAA AGGCATCTCG 
CTTTTGGTGC GAGCCGAATC CGATGGATCT CTACTTTTGG AGAGCAAGAT ACAACCGGAC 
ACCGCATACC AGAAGCAACA GGATACGCTG ATCGTGTGGT CCGAGGGGGA TAACTTTGAT 
CTGGCCCTGA GTTTCCAGGA AAAGGCGGGC TGCGACGAAA TATGGGAAAA GATATGTCAG 
GTGCAAGGCA AGGATCCATC TGTAGAAATA ACTCAAGACA TCGTCGAAGA GTCTGAGGAT 
GAGCGGTTTG AGGACATGTC GGATACTGCG CCACCCATTG AGCTACCACC CTGCGAGCTT 
TCCCGGCTGG AGGACATCTC GGAAACGATA CAAAGCTGTC TCTCCACGCC GCTGCGAAAG 
GAAAAGTTGT CGATGGCTCT GGAGTCGGAG AGCTATATCA AGAAGCTGCT GAACTTGTTC 
CACGTGTGTG AGGACCTGGA CAATACCGAG GGCCTGCATC ACCTTTTTGA GATCTTCAAG 
AACATCTTCT TGCTAAATAA GAACGCGCTC TTTGAGATTA TGTTTGCGGA TGATACCATC 
TTTGATGTGG TCGGGTGCCT GGAGTACGAT CCGAGTGTGT CTCAGCCCAA AAAGCATCGA 
CAGTATCTGA AGCAGTTAGC CAAGTTCCGC GAGGCAGTGC CGATTAAAAA CCTGGACCTG 
CTGGCTAAGA TTCACCAGAC GTTCCGGGTG CAGTACATCC AGGACATTAT CCTGCCCACA 
CCATCGGTCT TTGTCGAAGA CAACATGCTG AACACCCTGT CCAGCTTCAT CTTCTTCAAC 
AAGGTGGAGA TTGTCACGAT GATCCAGGAC GACGAGCGGT ATCTCCTGGA TGTGTTTGCT 
GTTCTCACCG ATCCCACCAC CGGAGACGCG AAACGCCGCG ACACAGTTCT CTTCCTCAAG 
GAGTTCTGCA ACTATGCCCA GAACCTGCAG CCGCAGGGCA AGGACTCGTT CTACAAAACT 
CTCACCTGCC TAGGCATTCT GCAGGCGCTG GAACTCACTC TCGTGATGAA CGACAAGAAG 
ACCAAATCGG CCTCCATCGA CATCCTCACG GCCATCGTAG AGTTCTCGCC TCTGGTAGTG 
CGCAATTACA CGCTCAACCA GGCCAATCGG CCAGAAGTGG AGCGTATGCT ACTGAACATT 
GCCATCGAAC AAATGCTGAA TGACTCGGAG CCGGAGCTTG GAATTGCGGT GCAGCTAATG 
GGTATCGTCA AGATTCTGTT GGAGCCGGAA AACATGCTCA CCGAGAAGGG CGATTTTCTC 
AACTTCTTTT ACAAATACAG TGTGCAAACG TTAGTGGCCC CGGTGATACT TAACACGATA 
GGCGACCGTC CGCAAAACGA GGACTATCAG ACGGCCCAGC TGCTGGGAAT CGTCCTGGAC 
ATTCTTTCGT TCTGCGTGGA ACACCACAGT TATCACATTA AAAACTTTTT GTTGCAAAAG 
GATCTCCTCA AGCGAATACT GGTGCTAATG AAGAGCACAC ATACGTTCCT TGTCCTCGGC 
GCTCTGCGAT TACTGCGCAA GATAATTGCA CTTAAAGATG AGTTTTACAA TAGACACATT 
GTCAAGTGCA ATCTGTTTGC GCCGGTGGTA GATGCCTTCA TTCGCAACAA CGGCCGATAC 
AACCTTCTGG AGTCAGCAAT TCTGGAGCTG TTTGAGTTTA TAAAACTCGA GGATATCCGC 
ACTCTGTGCG TTTACTTCGT GGAGAACTTC AGCAAGATAT TTGATGAAAT CGAATACGTG 
CAGACATTTA AGTACTTGAA GAATCGCTAC GATCAGTACC AGGATCGGCT TAAGGACCGC 
GACAAGATGG AAAACCGAAC TGACGGTGGC CTGCCCATCA TCCGTAGCGG TGGCCGGTTT 
CGTCGGGATC AGCGGCAAAT GGAGGAGGAA GAGGAAATGT GGTTTAACGA GGAGGACGAT 
TTCACCGAGG AAATCGACAC ATACAACAAT GTCATGAAAT CCGTCAGCGA AAAGAATGGC 
CCGCAAACGC AAAACCAGCA GAAATCCTCT CCGCCACACA GCACGTCGCC GCACAGTGGA 
TTGTTGGGAA GCCTGAGCAC CACCGCGTCA TCCACTGCCA CATCCGCCAC GTCAGGTGCA 
CCGGTGGCCA GCGGCAGCAG TAGCCCCGAG GCCATCTCCG CCGACGAGCA GACGCAGGCG 
GCGGTGCATT TGGCTGCCGC TGCCCTTCAG CACCACCAGC AACAGCAACA GCAGCAGCAG 
CAGAACCCAT TCCAGCAACA GACACAGCCC GAGATCGCGG AGCTACAACA GCAGCTGAGC 
AGCGTGGAAG CGCCTCAAAG CCAGGAGTTG GAGCTGTCAC AATCGGCCGC CGCCAGTGCA 
TCGCCCACTT CGTCATCCTC GTCTCTGGAA GCCTCGACGT CCTCTTCATC GGCGTCATCG 
TCTTCATCCT CGTCGTCCTC GTCCTCGCCG CCCGGCTCAT CGGCCGCAGC ATCGCTATGC 
GATTCGGCGA CAGTAGCCGC CGTGGCAGCA TCACAGTTTC TCTCAACGAT CGCCACAGCC 
ATGGCGGCCT CTGTGACGGC GGCGGCGGCA ACAAACAGCT CCCCGAGCAT TTCGCCAGCT 
CCAGCTGTGT CCAGTCCGGA TATCGAGAAC GCCGACGCCC AGTTGCCGCC CAGCGATGAC 
GCCAGCAGCC CGGCCAGTGG GGAGCAAGAC GCTAACAGCA CGGAAGGTAC CAGCAGTGAG 
GCCGACAAGA CGACGGCCAA AAAGGGTCTG GTGGACTATG AGAGTGATTC TGGCGAAGAT 
GACTACGAGG AGGATGAGTA CTCCGAGGGG CCACAAGCAC AAAAGCGCGC GCGTCAGGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731850.2
CDS	625..3567
Translation	MTTDTRRRVKLYALNAERQWDDRGTGHVSSTYVERLKGISLLVRAESDGSLLLESKIQPDTAYQKQQDTLIVWSEGDNFDLALSFQEKAGCDEIWEKICQVQGKDPSVEITQDIVEESEDERFEDMSDTAPPIELPPCELSRLEDISETIQSCLSTPLRKEKLSMALESESYIKKLLNLFHVCEDLDNTEGLHHLFEIFKNIFLLNKNALFEIMFADDTIFDVVGCLEYDPSVSQPKKHRQYLKQLAKFREAVPIKNLDLLAKIHQTFRVQYIQDIILPTPSVFVEDNMLNTLSSFIFFNKVEIVTMIQDDERYLLDVFAVLTDPTTGDAKRRDTVLFLKEFCNYAQNLQPQGKDSFYKTLTCLGILQALELTLVMNDKKTKSASIDILTAIVEFSPLVVRNYTLNQANRPEVERMLLNIAIEQMLNDSEPELGIAVQLMGIVKILLEPENMLTEKGDFLNFFYKYSVQTLVAPVILNTIGDRPQNEDYQTAQLLGIVLDILSFCVEHHSYHIKNFLLQKDLLKRILVLMKSTHTFLVLGALRLLRKIIALKDEFYNRHIVKCNLFAPVVDAFIRNNGRYNLLESAILELFEFIKLEDIRTLCVYFVENFSKIFDEIEYVQTFKYLKNRYDQYQDRLKDRDKMENRTDGGLPIIRSGGRFRRDQRQMEEEEEMWFNEEDDFTEEIDTYNNVMKSVSEKNGPQTQNQQKSSPPHSTSPHSGLLGSLSTTASSTATSATSGAPVASGSSSPEAISADEQTQAAVHLAAAALQHHQQQQQQQQQNPFQQQTQPEIAELQQQLSSVEAPQSQELELSQSAAASASPTSSSSSLEASTSSSSASSSSSSSSSSSPPGSSAAASLCDSATVAAVAASQFLSTIATAMAASVTAAAATNSSPSISPAPAVSSPDIENADAQLPPSDDASSPASGEQDANSTEGTSSEADKTTAKKGLVDYESDSGEDDYEEDEYSEGPQAQKRARQA

Target ORF information:

RefSeq Version	NM_169549.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster falafel (flfl), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169549.2

ORF Insert Sequence:

ATGACGACTG ACACCCGCCG ACGCGTCAAG CTGTACGCGC TGAATGCGGA ACGGCAATGG 
GATGATCGTG GCACTGGCCA CGTGTCATCC ACCTATGTGG AAAGACTAAA AGGCATCTCG 
CTTTTGGTGC GAGCCGAATC CGATGGATCT CTACTTTTGG AGAGCAAGAT ACAACCGGAC 
ACCGCATACC AGAAGCAACA GGATACGCTG ATCGTGTGGT CCGAGGGGGA TAACTTTGAT 
CTGGCCCTGA GTTTCCAGGA AAAGGCGGGC TGCGACGAAA TATGGGAAAA GATATGTCAG 
GTGCAAGGCA AGGATCCATC TGTAGAAATA ACTCAAGACA TCGTCGAAGA GTCTGAGGAT 
GAGCGGTTTG AGGACATGTC GGATACTGCG CCACCCATTG AGCTACCACC CTGCGAGCTT 
TCCCGGCTGG AGGACATCTC GGAAACGATA CAAAGCTGTC TCTCCACGCC GCTGCGAAAG 
GAAAAGTTGT CGATGGCTCT GGAGTCGGAG AGCTATATCA AGAAGCTGCT GAACTTGTTC 
CACGTGTGTG AGGACCTGGA CAATACCGAG GGCCTGCATC ACCTTTTTGA GATCTTCAAG 
AACATCTTCT TGCTAAATAA GAACGCGCTC TTTGAGATTA TGTTTGCGGA TGATACCATC 
TTTGATGTGG TCGGGTGCCT GGAGTACGAT CCGAGTGTGT CTCAGCCCAA AAAGCATCGA 
CAGTATCTGA AGCAGTTAGC CAAGTTCCGC GAGGCAGTGC CGATTAAAAA CCTGGACCTG 
CTGGCTAAGA TTCACCAGAC GTTCCGGGTG CAGTACATCC AGGACATTAT CCTGCCCACA 
CCATCGGTCT TTGTCGAAGA CAACATGCTG AACACCCTGT CCAGCTTCAT CTTCTTCAAC 
AAGGTGGAGA TTGTCACGAT GATCCAGGAC GACGAGCGGT ATCTCCTGGA TGTGTTTGCT 
GTTCTCACCG ATCCCACCAC CGGAGACGCG AAACGCCGCG ACACAGTTCT CTTCCTCAAG 
GAGTTCTGCA ACTATGCCCA GAACCTGCAG CCGCAGGGCA AGGACTCGTT CTACAAAACT 
CTCACCTGCC TAGGCATTCT GCAGGCGCTG GAACTCACTC TCGTGATGAA CGACAAGAAG 
ACCAAATCGG CCTCCATCGA CATCCTCACG GCCATCGTAG AGTTCTCGCC TCTGGTAGTG 
CGCAATTACA CGCTCAACCA GGCCAATCGG CCAGAAGTGG AGCGTATGCT ACTGAACATT 
GCCATCGAAC AAATGCTGAA TGACTCGGAG CCGGAGCTTG GAATTGCGGT GCAGCTAATG 
GGTATCGTCA AGATTCTGTT GGAGCCGGAA AACATGCTCA CCGAGAAGGG CGATTTTCTC 
AACTTCTTTT ACAAATACAG TGTGCAAACG TTAGTGGCCC CGGTGATACT TAACACGATA 
GGCGACCGTC CGCAAAACGA GGACTATCAG ACGGCCCAGC TGCTGGGAAT CGTCCTGGAC 
ATTCTTTCGT TCTGCGTGGA ACACCACAGT TATCACATTA AAAACTTTTT GTTGCAAAAG 
GATCTCCTCA AGCGAATACT GGTGCTAATG AAGAGCACAC ATACGTTCCT TGTCCTCGGC 
GCTCTGCGAT TACTGCGCAA GATAATTGCA CTTAAAGATG AGTTTTACAA TAGACACATT 
GTCAAGTGCA ATCTGTTTGC GCCGGTGGTA GATGCCTTCA TTCGCAACAA CGGCCGATAC 
AACCTTCTGG AGTCAGCAAT TCTGGAGCTG TTTGAGTTTA TAAAACTCGA GGATATCCGC 
ACTCTGTGCG TTTACTTCGT GGAGAACTTC AGCAAGATAT TTGATGAAAT CGAATACGTG 
CAGACATTTA AGTACTTGAA GAATCGCTAC GATCAGTACC AGGATCGGCT TAAGGACCGC 
GACAAGATGG AAAACCGAAC TGACGGTGGC CTGCCCATCA TCCGTAGCGG TGGCCGGTTT 
CGTCGGGATC AGCGGCAAAT GGAGGAGGAA GAGGAAATGT GGTTTAACGA GGAGGACGAT 
TTCACCGAGG AAATCGACAC ATACAACAAT GTCATGAAAT CCGTCAGCGA AAAGAATGGC 
CCGCAAACGC AAAACCAGCA GAAATCCTCT CCGCCACACA GCACGTCGCC GCACAGTGGA 
TTGTTGGGAA GCCTGAGCAC CACCGCGTCA TCCACTGCCA CATCCGCCAC GTCAGGTGCA 
CCGGTGGCCA GCGGCAGCAG TAGCCCCGAG GCCATCTCCG CCGACGAGCA GACGCAGGCG 
GCGGTGCATT TGGCTGCCGC TGCCCTTCAG CACCACCAGC AACAGCAACA GCAGCAGCAG 
CAGAACCCAT TCCAGCAACA GACACAGCCC GAGATCGCGG AGCTACAACA GCAGCTGAGC 
AGCGTGGAAG CGCCTCAAAG CCAGGAGTTG GAGCTGTCAC AATCGGCCGC CGCCAGTGCA 
TCGCCCACTT CGTCATCCTC GTCTCTGGAA GCCTCGACGT CCTCTTCATC GGCGTCATCG 
TCTTCATCCT CGTCGTCCTC GTCCTCGCCG CCCGGCTCAT CGGCCGCAGC ATCGCTATGC 
GATTCGGCGA CAGTAGCCGC CGTGGCAGCA TCACAGTTTC TCTCAACGAT CGCCACAGCC 
ATGGCGGCCT CTGTGACGGC GGCGGCGGCA ACAAACAGCT CCCCGAGCAT TTCGCCAGCT 
CCAGCTGTGT CCAGTCCGGA TATCGAGAAC GCCGACGCCC AGTTGCCGCC CAGCGATGAC 
GCCAGCAGCC CGGCCAGTGG GGAGCAAGAC GCTAACAGCA CGGAAGGTAC CAGCAGTGAG 
GCCGACAAGA CGACGGCCAA AAAGGGTCTG GTGGACTATG AGAGTGATTC TGGCGAAGAT 
GACTACGAGG AGGATGAGTA CTCCGAGGGG CCACAAGCAC AAAAGCGCGC GCGTCAGGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flfl ( NM_169548.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731849.2 Drosophila melanogaster falafel (flfl), transcript variant B, mRNA.

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CloneID	OFa08349
Clone ID Related Accession (Same CDS sequence)	NM_169549.2 , NM_169548.3
Accession Version	NM_169548.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	falafel, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169548.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACTG ACACCCGCCG ACGCGTCAAG CTGTACGCGC TGAATGCGGA ACGGCAATGG 
GATGATCGTG GCACTGGCCA CGTGTCATCC ACCTATGTGG AAAGACTAAA AGGCATCTCG 
CTTTTGGTGC GAGCCGAATC CGATGGATCT CTACTTTTGG AGAGCAAGAT ACAACCGGAC 
ACCGCATACC AGAAGCAACA GGATACGCTG ATCGTGTGGT CCGAGGGGGA TAACTTTGAT 
CTGGCCCTGA GTTTCCAGGA AAAGGCGGGC TGCGACGAAA TATGGGAAAA GATATGTCAG 
GTGCAAGGCA AGGATCCATC TGTAGAAATA ACTCAAGACA TCGTCGAAGA GTCTGAGGAT 
GAGCGGTTTG AGGACATGTC GGATACTGCG CCACCCATTG AGCTACCACC CTGCGAGCTT 
TCCCGGCTGG AGGACATCTC GGAAACGATA CAAAGCTGTC TCTCCACGCC GCTGCGAAAG 
GAAAAGTTGT CGATGGCTCT GGAGTCGGAG AGCTATATCA AGAAGCTGCT GAACTTGTTC 
CACGTGTGTG AGGACCTGGA CAATACCGAG GGCCTGCATC ACCTTTTTGA GATCTTCAAG 
AACATCTTCT TGCTAAATAA GAACGCGCTC TTTGAGATTA TGTTTGCGGA TGATACCATC 
TTTGATGTGG TCGGGTGCCT GGAGTACGAT CCGAGTGTGT CTCAGCCCAA AAAGCATCGA 
CAGTATCTGA AGCAGTTAGC CAAGTTCCGC GAGGCAGTGC CGATTAAAAA CCTGGACCTG 
CTGGCTAAGA TTCACCAGAC GTTCCGGGTG CAGTACATCC AGGACATTAT CCTGCCCACA 
CCATCGGTCT TTGTCGAAGA CAACATGCTG AACACCCTGT CCAGCTTCAT CTTCTTCAAC 
AAGGTGGAGA TTGTCACGAT GATCCAGGAC GACGAGCGGT ATCTCCTGGA TGTGTTTGCT 
GTTCTCACCG ATCCCACCAC CGGAGACGCG AAACGCCGCG ACACAGTTCT CTTCCTCAAG 
GAGTTCTGCA ACTATGCCCA GAACCTGCAG CCGCAGGGCA AGGACTCGTT CTACAAAACT 
CTCACCTGCC TAGGCATTCT GCAGGCGCTG GAACTCACTC TCGTGATGAA CGACAAGAAG 
ACCAAATCGG CCTCCATCGA CATCCTCACG GCCATCGTAG AGTTCTCGCC TCTGGTAGTG 
CGCAATTACA CGCTCAACCA GGCCAATCGG CCAGAAGTGG AGCGTATGCT ACTGAACATT 
GCCATCGAAC AAATGCTGAA TGACTCGGAG CCGGAGCTTG GAATTGCGGT GCAGCTAATG 
GGTATCGTCA AGATTCTGTT GGAGCCGGAA AACATGCTCA CCGAGAAGGG CGATTTTCTC 
AACTTCTTTT ACAAATACAG TGTGCAAACG TTAGTGGCCC CGGTGATACT TAACACGATA 
GGCGACCGTC CGCAAAACGA GGACTATCAG ACGGCCCAGC TGCTGGGAAT CGTCCTGGAC 
ATTCTTTCGT TCTGCGTGGA ACACCACAGT TATCACATTA AAAACTTTTT GTTGCAAAAG 
GATCTCCTCA AGCGAATACT GGTGCTAATG AAGAGCACAC ATACGTTCCT TGTCCTCGGC 
GCTCTGCGAT TACTGCGCAA GATAATTGCA CTTAAAGATG AGTTTTACAA TAGACACATT 
GTCAAGTGCA ATCTGTTTGC GCCGGTGGTA GATGCCTTCA TTCGCAACAA CGGCCGATAC 
AACCTTCTGG AGTCAGCAAT TCTGGAGCTG TTTGAGTTTA TAAAACTCGA GGATATCCGC 
ACTCTGTGCG TTTACTTCGT GGAGAACTTC AGCAAGATAT TTGATGAAAT CGAATACGTG 
CAGACATTTA AGTACTTGAA GAATCGCTAC GATCAGTACC AGGATCGGCT TAAGGACCGC 
GACAAGATGG AAAACCGAAC TGACGGTGGC CTGCCCATCA TCCGTAGCGG TGGCCGGTTT 
CGTCGGGATC AGCGGCAAAT GGAGGAGGAA GAGGAAATGT GGTTTAACGA GGAGGACGAT 
TTCACCGAGG AAATCGACAC ATACAACAAT GTCATGAAAT CCGTCAGCGA AAAGAATGGC 
CCGCAAACGC AAAACCAGCA GAAATCCTCT CCGCCACACA GCACGTCGCC GCACAGTGGA 
TTGTTGGGAA GCCTGAGCAC CACCGCGTCA TCCACTGCCA CATCCGCCAC GTCAGGTGCA 
CCGGTGGCCA GCGGCAGCAG TAGCCCCGAG GCCATCTCCG CCGACGAGCA GACGCAGGCG 
GCGGTGCATT TGGCTGCCGC TGCCCTTCAG CACCACCAGC AACAGCAACA GCAGCAGCAG 
CAGAACCCAT TCCAGCAACA GACACAGCCC GAGATCGCGG AGCTACAACA GCAGCTGAGC 
AGCGTGGAAG CGCCTCAAAG CCAGGAGTTG GAGCTGTCAC AATCGGCCGC CGCCAGTGCA 
TCGCCCACTT CGTCATCCTC GTCTCTGGAA GCCTCGACGT CCTCTTCATC GGCGTCATCG 
TCTTCATCCT CGTCGTCCTC GTCCTCGCCG CCCGGCTCAT CGGCCGCAGC ATCGCTATGC 
GATTCGGCGA CAGTAGCCGC CGTGGCAGCA TCACAGTTTC TCTCAACGAT CGCCACAGCC 
ATGGCGGCCT CTGTGACGGC GGCGGCGGCA ACAAACAGCT CCCCGAGCAT TTCGCCAGCT 
CCAGCTGTGT CCAGTCCGGA TATCGAGAAC GCCGACGCCC AGTTGCCGCC CAGCGATGAC 
GCCAGCAGCC CGGCCAGTGG GGAGCAAGAC GCTAACAGCA CGGAAGGTAC CAGCAGTGAG 
GCCGACAAGA CGACGGCCAA AAAGGGTCTG GTGGACTATG AGAGTGATTC TGGCGAAGAT 
GACTACGAGG AGGATGAGTA CTCCGAGGGG CCACAAGCAC AAAAGCGCGC GCGTCAGGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731849.2
CDS	525..3467
Translation	MTTDTRRRVKLYALNAERQWDDRGTGHVSSTYVERLKGISLLVRAESDGSLLLESKIQPDTAYQKQQDTLIVWSEGDNFDLALSFQEKAGCDEIWEKICQVQGKDPSVEITQDIVEESEDERFEDMSDTAPPIELPPCELSRLEDISETIQSCLSTPLRKEKLSMALESESYIKKLLNLFHVCEDLDNTEGLHHLFEIFKNIFLLNKNALFEIMFADDTIFDVVGCLEYDPSVSQPKKHRQYLKQLAKFREAVPIKNLDLLAKIHQTFRVQYIQDIILPTPSVFVEDNMLNTLSSFIFFNKVEIVTMIQDDERYLLDVFAVLTDPTTGDAKRRDTVLFLKEFCNYAQNLQPQGKDSFYKTLTCLGILQALELTLVMNDKKTKSASIDILTAIVEFSPLVVRNYTLNQANRPEVERMLLNIAIEQMLNDSEPELGIAVQLMGIVKILLEPENMLTEKGDFLNFFYKYSVQTLVAPVILNTIGDRPQNEDYQTAQLLGIVLDILSFCVEHHSYHIKNFLLQKDLLKRILVLMKSTHTFLVLGALRLLRKIIALKDEFYNRHIVKCNLFAPVVDAFIRNNGRYNLLESAILELFEFIKLEDIRTLCVYFVENFSKIFDEIEYVQTFKYLKNRYDQYQDRLKDRDKMENRTDGGLPIIRSGGRFRRDQRQMEEEEEMWFNEEDDFTEEIDTYNNVMKSVSEKNGPQTQNQQKSSPPHSTSPHSGLLGSLSTTASSTATSATSGAPVASGSSSPEAISADEQTQAAVHLAAAALQHHQQQQQQQQQNPFQQQTQPEIAELQQQLSSVEAPQSQELELSQSAAASASPTSSSSSLEASTSSSSASSSSSSSSSSSPPGSSAAASLCDSATVAAVAASQFLSTIATAMAASVTAAAATNSSPSISPAPAVSSPDIENADAQLPPSDDASSPASGEQDANSTEGTSSEADKTTAKKGLVDYESDSGEDDYEEDEYSEGPQAQKRARQA

Target ORF information:

RefSeq Version	NM_169548.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster falafel (flfl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169548.3

ORF Insert Sequence:

ATGACGACTG ACACCCGCCG ACGCGTCAAG CTGTACGCGC TGAATGCGGA ACGGCAATGG 
GATGATCGTG GCACTGGCCA CGTGTCATCC ACCTATGTGG AAAGACTAAA AGGCATCTCG 
CTTTTGGTGC GAGCCGAATC CGATGGATCT CTACTTTTGG AGAGCAAGAT ACAACCGGAC 
ACCGCATACC AGAAGCAACA GGATACGCTG ATCGTGTGGT CCGAGGGGGA TAACTTTGAT 
CTGGCCCTGA GTTTCCAGGA AAAGGCGGGC TGCGACGAAA TATGGGAAAA GATATGTCAG 
GTGCAAGGCA AGGATCCATC TGTAGAAATA ACTCAAGACA TCGTCGAAGA GTCTGAGGAT 
GAGCGGTTTG AGGACATGTC GGATACTGCG CCACCCATTG AGCTACCACC CTGCGAGCTT 
TCCCGGCTGG AGGACATCTC GGAAACGATA CAAAGCTGTC TCTCCACGCC GCTGCGAAAG 
GAAAAGTTGT CGATGGCTCT GGAGTCGGAG AGCTATATCA AGAAGCTGCT GAACTTGTTC 
CACGTGTGTG AGGACCTGGA CAATACCGAG GGCCTGCATC ACCTTTTTGA GATCTTCAAG 
AACATCTTCT TGCTAAATAA GAACGCGCTC TTTGAGATTA TGTTTGCGGA TGATACCATC 
TTTGATGTGG TCGGGTGCCT GGAGTACGAT CCGAGTGTGT CTCAGCCCAA AAAGCATCGA 
CAGTATCTGA AGCAGTTAGC CAAGTTCCGC GAGGCAGTGC CGATTAAAAA CCTGGACCTG 
CTGGCTAAGA TTCACCAGAC GTTCCGGGTG CAGTACATCC AGGACATTAT CCTGCCCACA 
CCATCGGTCT TTGTCGAAGA CAACATGCTG AACACCCTGT CCAGCTTCAT CTTCTTCAAC 
AAGGTGGAGA TTGTCACGAT GATCCAGGAC GACGAGCGGT ATCTCCTGGA TGTGTTTGCT 
GTTCTCACCG ATCCCACCAC CGGAGACGCG AAACGCCGCG ACACAGTTCT CTTCCTCAAG 
GAGTTCTGCA ACTATGCCCA GAACCTGCAG CCGCAGGGCA AGGACTCGTT CTACAAAACT 
CTCACCTGCC TAGGCATTCT GCAGGCGCTG GAACTCACTC TCGTGATGAA CGACAAGAAG 
ACCAAATCGG CCTCCATCGA CATCCTCACG GCCATCGTAG AGTTCTCGCC TCTGGTAGTG 
CGCAATTACA CGCTCAACCA GGCCAATCGG CCAGAAGTGG AGCGTATGCT ACTGAACATT 
GCCATCGAAC AAATGCTGAA TGACTCGGAG CCGGAGCTTG GAATTGCGGT GCAGCTAATG 
GGTATCGTCA AGATTCTGTT GGAGCCGGAA AACATGCTCA CCGAGAAGGG CGATTTTCTC 
AACTTCTTTT ACAAATACAG TGTGCAAACG TTAGTGGCCC CGGTGATACT TAACACGATA 
GGCGACCGTC CGCAAAACGA GGACTATCAG ACGGCCCAGC TGCTGGGAAT CGTCCTGGAC 
ATTCTTTCGT TCTGCGTGGA ACACCACAGT TATCACATTA AAAACTTTTT GTTGCAAAAG 
GATCTCCTCA AGCGAATACT GGTGCTAATG AAGAGCACAC ATACGTTCCT TGTCCTCGGC 
GCTCTGCGAT TACTGCGCAA GATAATTGCA CTTAAAGATG AGTTTTACAA TAGACACATT 
GTCAAGTGCA ATCTGTTTGC GCCGGTGGTA GATGCCTTCA TTCGCAACAA CGGCCGATAC 
AACCTTCTGG AGTCAGCAAT TCTGGAGCTG TTTGAGTTTA TAAAACTCGA GGATATCCGC 
ACTCTGTGCG TTTACTTCGT GGAGAACTTC AGCAAGATAT TTGATGAAAT CGAATACGTG 
CAGACATTTA AGTACTTGAA GAATCGCTAC GATCAGTACC AGGATCGGCT TAAGGACCGC 
GACAAGATGG AAAACCGAAC TGACGGTGGC CTGCCCATCA TCCGTAGCGG TGGCCGGTTT 
CGTCGGGATC AGCGGCAAAT GGAGGAGGAA GAGGAAATGT GGTTTAACGA GGAGGACGAT 
TTCACCGAGG AAATCGACAC ATACAACAAT GTCATGAAAT CCGTCAGCGA AAAGAATGGC 
CCGCAAACGC AAAACCAGCA GAAATCCTCT CCGCCACACA GCACGTCGCC GCACAGTGGA 
TTGTTGGGAA GCCTGAGCAC CACCGCGTCA TCCACTGCCA CATCCGCCAC GTCAGGTGCA 
CCGGTGGCCA GCGGCAGCAG TAGCCCCGAG GCCATCTCCG CCGACGAGCA GACGCAGGCG 
GCGGTGCATT TGGCTGCCGC TGCCCTTCAG CACCACCAGC AACAGCAACA GCAGCAGCAG 
CAGAACCCAT TCCAGCAACA GACACAGCCC GAGATCGCGG AGCTACAACA GCAGCTGAGC 
AGCGTGGAAG CGCCTCAAAG CCAGGAGTTG GAGCTGTCAC AATCGGCCGC CGCCAGTGCA 
TCGCCCACTT CGTCATCCTC GTCTCTGGAA GCCTCGACGT CCTCTTCATC GGCGTCATCG 
TCTTCATCCT CGTCGTCCTC GTCCTCGCCG CCCGGCTCAT CGGCCGCAGC ATCGCTATGC 
GATTCGGCGA CAGTAGCCGC CGTGGCAGCA TCACAGTTTC TCTCAACGAT CGCCACAGCC 
ATGGCGGCCT CTGTGACGGC GGCGGCGGCA ACAAACAGCT CCCCGAGCAT TTCGCCAGCT 
CCAGCTGTGT CCAGTCCGGA TATCGAGAAC GCCGACGCCC AGTTGCCGCC CAGCGATGAC 
GCCAGCAGCC CGGCCAGTGG GGAGCAAGAC GCTAACAGCA CGGAAGGTAC CAGCAGTGAG 
GCCGACAAGA CGACGGCCAA AAAGGGTCTG GTGGACTATG AGAGTGATTC TGGCGAAGAT 
GACTACGAGG AGGATGAGTA CTCCGAGGGG CCACAAGCAC AAAAGCGCGC GCGTCAGGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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flfl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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