CCHa2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCHa2
Entrez Gene ID	41648
Full Name	CCHamide-2
Synonyms	BcDNA:RE10132,CCH2,CCM,CG14375,Dm-CCHa2,Dmel\CG14375
General protein information	Preferred Names CCHamide-2 Names CG14375 gene product from transcript CG14375-RA CCHa2-PA CCHa2-PB CCHa2-PC CCHamide CCHamide 2 CG14375-PA CG14375-PB CG14375-PC Drm-CCHamide-2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87E8-87E8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001202287.1	NP_001189216.1	CCHamide-2, isoform B
NM_001202288.1	NP_001189217.1	CCHamide-2, isoform C
NM_142028.3	NP_650285.1	CCHamide-2, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

genetic disruption of either CCHa2 or CCHa2-R caused almost identical defects in larval growth and developmental timing
Title: The Nutrient-Responsive Hormone CCHamide-2 Controls Growth by Regulating Insulin-like Peptides in the Brain of Drosophila melanogaster.

Data indicate that the activation of CCHa2/CCHa2-R signaling promotes the sensation of good nutritional status in the larval brain, leading to Dilp-dependent tissue growth.
Title: Coupling of growth to nutritional status: The role of novel periphery-to-brain signaling by the CCHa2 peptide in Drosophila melanogaster.

conclude that CCHamide-2 is an orexigenic peptide and an important factor for controlling developmental timing in Drosophila
Title: CCHamide-2 Is an Orexigenic Brain-Gut Peptide in Drosophila.

orcokinin B, CCHamide 1, and CCHamide 2 expression in Drosophila is reported.
Title: More Drosophila enteroendocrine peptides: Orcokinin B and the CCHamides 1 and 2.

Expression patterns of the Drosophila neuropeptide CCHamide-2 and its receptor may suggest hormonal signaling from the gut to the brain.
Title: Expression patterns of the Drosophila neuropeptide CCHamide-2 and its receptor may suggest hormonal signaling from the gut to the brain.

The following CCHa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCHa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04802	NM_142028.3 Latest version!	Drosophila melanogaster CCHamide-2 (CCHa2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa04802	NM_001202287.1 Latest version!	Drosophila melanogaster CCHamide-2 (CCHa2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa04802	NM_001202288.1 Latest version!	Drosophila melanogaster CCHamide-2 (CCHa2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCHa2 ( NM_001202287.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189216.1 Drosophila melanogaster CCHamide-2 (CCHa2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04802
Clone ID Related Accession (Same CDS sequence)	NM_001202287.1 , NM_001202288.1 , NM_142028.3
Accession Version	NM_001202287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CCHamide-2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAATCCA CCATATCTTT GCTACTGGTC GTTATCTGCA CCGTGGTCCT GGCCGCGCAA 
CAGAGCCAAG CGAAAAAGGG ATGCCAGGCC TACGGTCATG TGTGCTACGG TGGTCATGGC 
AAGCGCTCTC TGAGCCCCGG ATCCGGGTCT GGAACGGGAG TGGGCGGGGG AATGGGCGAG 
GCAGCCTCCG GGGGGCAGGA ACCGGACTAT GTGCGTCCGA ATGGCCTACT GCCCATGATG 
GCGCCAAATG AACAGGTGCC GCTCGAGGGC GACTTTAATG ACTATCCCGC CCGTCAGGTG 
CTTTACAAAA TCATGAAATC ATGGTTTAAC CGACCTCGCC GACCCGCCTC TCGCCTGGGT 
GAACTTGATT ATCCCTTGGC CAATTCCGCC GAATTGAATG GCGTGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189216.1
CDS	257..667
Translation	MKSTISLLLVVICTVVLAAQQSQAKKGCQAYGHVCYGGHGKRSLSPGSGSGTGVGGGMGEAASGGQEPDYVRPNGLLPMMAPNEQVPLEGDFNDYPARQVLYKIMKSWFNRPRRPASRLGELDYPLANSAELNGVN

Target ORF information:

RefSeq Version	NM_001202287.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CCHamide-2 (CCHa2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202287.1

ORF Insert Sequence:

ATGAAATCCA CCATATCTTT GCTACTGGTC GTTATCTGCA CCGTGGTCCT GGCCGCGCAA 
CAGAGCCAAG CGAAAAAGGG ATGCCAGGCC TACGGTCATG TGTGCTACGG TGGTCATGGC 
AAGCGCTCTC TGAGCCCCGG ATCCGGGTCT GGAACGGGAG TGGGCGGGGG AATGGGCGAG 
GCAGCCTCCG GGGGGCAGGA ACCGGACTAT GTGCGTCCGA ATGGCCTACT GCCCATGATG 
GCGCCAAATG AACAGGTGCC GCTCGAGGGC GACTTTAATG ACTATCCCGC CCGTCAGGTG 
CTTTACAAAA TCATGAAATC ATGGTTTAAC CGACCTCGCC GACCCGCCTC TCGCCTGGGT 
GAACTTGATT ATCCCTTGGC CAATTCCGCC GAATTGAATG GCGTGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCHa2 ( NM_001202288.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189217.1 Drosophila melanogaster CCHamide-2 (CCHa2), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04802
Clone ID Related Accession (Same CDS sequence)	NM_001202287.1 , NM_001202288.1 , NM_142028.3
Accession Version	NM_001202288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CCHamide-2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAATCCA CCATATCTTT GCTACTGGTC GTTATCTGCA CCGTGGTCCT GGCCGCGCAA 
CAGAGCCAAG CGAAAAAGGG ATGCCAGGCC TACGGTCATG TGTGCTACGG TGGTCATGGC 
AAGCGCTCTC TGAGCCCCGG ATCCGGGTCT GGAACGGGAG TGGGCGGGGG AATGGGCGAG 
GCAGCCTCCG GGGGGCAGGA ACCGGACTAT GTGCGTCCGA ATGGCCTACT GCCCATGATG 
GCGCCAAATG AACAGGTGCC GCTCGAGGGC GACTTTAATG ACTATCCCGC CCGTCAGGTG 
CTTTACAAAA TCATGAAATC ATGGTTTAAC CGACCTCGCC GACCCGCCTC TCGCCTGGGT 
GAACTTGATT ATCCCTTGGC CAATTCCGCC GAATTGAATG GCGTGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189217.1
CDS	298..708
Translation	MKSTISLLLVVICTVVLAAQQSQAKKGCQAYGHVCYGGHGKRSLSPGSGSGTGVGGGMGEAASGGQEPDYVRPNGLLPMMAPNEQVPLEGDFNDYPARQVLYKIMKSWFNRPRRPASRLGELDYPLANSAELNGVN

Target ORF information:

RefSeq Version	NM_001202288.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CCHamide-2 (CCHa2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202288.1

ORF Insert Sequence:

ATGAAATCCA CCATATCTTT GCTACTGGTC GTTATCTGCA CCGTGGTCCT GGCCGCGCAA 
CAGAGCCAAG CGAAAAAGGG ATGCCAGGCC TACGGTCATG TGTGCTACGG TGGTCATGGC 
AAGCGCTCTC TGAGCCCCGG ATCCGGGTCT GGAACGGGAG TGGGCGGGGG AATGGGCGAG 
GCAGCCTCCG GGGGGCAGGA ACCGGACTAT GTGCGTCCGA ATGGCCTACT GCCCATGATG 
GCGCCAAATG AACAGGTGCC GCTCGAGGGC GACTTTAATG ACTATCCCGC CCGTCAGGTG 
CTTTACAAAA TCATGAAATC ATGGTTTAAC CGACCTCGCC GACCCGCCTC TCGCCTGGGT 
GAACTTGATT ATCCCTTGGC CAATTCCGCC GAATTGAATG GCGTGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCHa2 ( NM_142028.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650285.1 Drosophila melanogaster CCHamide-2 (CCHa2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04802
Clone ID Related Accession (Same CDS sequence)	NM_001202287.1 , NM_001202288.1 , NM_142028.3
Accession Version	NM_142028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CCHamide-2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142028.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAATCCA CCATATCTTT GCTACTGGTC GTTATCTGCA CCGTGGTCCT GGCCGCGCAA 
CAGAGCCAAG CGAAAAAGGG ATGCCAGGCC TACGGTCATG TGTGCTACGG TGGTCATGGC 
AAGCGCTCTC TGAGCCCCGG ATCCGGGTCT GGAACGGGAG TGGGCGGGGG AATGGGCGAG 
GCAGCCTCCG GGGGGCAGGA ACCGGACTAT GTGCGTCCGA ATGGCCTACT GCCCATGATG 
GCGCCAAATG AACAGGTGCC GCTCGAGGGC GACTTTAATG ACTATCCCGC CCGTCAGGTG 
CTTTACAAAA TCATGAAATC ATGGTTTAAC CGACCTCGCC GACCCGCCTC TCGCCTGGGT 
GAACTTGATT ATCCCTTGGC CAATTCCGCC GAATTGAATG GCGTGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650285.1
CDS	143..553
Translation	MKSTISLLLVVICTVVLAAQQSQAKKGCQAYGHVCYGGHGKRSLSPGSGSGTGVGGGMGEAASGGQEPDYVRPNGLLPMMAPNEQVPLEGDFNDYPARQVLYKIMKSWFNRPRRPASRLGELDYPLANSAELNGVN

Target ORF information:

RefSeq Version	NM_142028.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CCHamide-2 (CCHa2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142028.3

ORF Insert Sequence:

ATGAAATCCA CCATATCTTT GCTACTGGTC GTTATCTGCA CCGTGGTCCT GGCCGCGCAA 
CAGAGCCAAG CGAAAAAGGG ATGCCAGGCC TACGGTCATG TGTGCTACGG TGGTCATGGC 
AAGCGCTCTC TGAGCCCCGG ATCCGGGTCT GGAACGGGAG TGGGCGGGGG AATGGGCGAG 
GCAGCCTCCG GGGGGCAGGA ACCGGACTAT GTGCGTCCGA ATGGCCTACT GCCCATGATG 
GCGCCAAATG AACAGGTGCC GCTCGAGGGC GACTTTAATG ACTATCCCGC CCGTCAGGTG 
CTTTACAAAA TCATGAAATC ATGGTTTAAC CGACCTCGCC GACCCGCCTC TCGCCTGGGT 
GAACTTGATT ATCCCTTGGC CAATTCCGCC GAATTGAATG GCGTGAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CCHa2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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