CG32473 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG32473
Entrez Gene ID	41636
Full Name	CG32473 gene product from transcript CG32473-RA
Synonyms	CG11688,CG8775,Dmel\CG32473,GM08240p
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87E5-87E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_169503.3	NP_731786.1	uncharacterized protein, isoform A
NM_142019.3	NP_650276.2	uncharacterized protein, isoform B
NM_169504.3	NP_731787.1	uncharacterized protein, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	ENPEP	NP_001968.3
	ENPEP	XP_001090456.1
Mus musculus (house mouse)	Enpep	NP_031960.1
Drosophila melanogaster (fruit fly)	CG32473	NP_731786.1
Gallus gallus (chicken)	ENPEP	XP_426327.3
Xenopus tropicalis (tropical clawed frog)	enpep	XP_002934295.1
Pan troglodytes (chimpanzee)	ENPEP	XP_517397.2
Canis lupus familiaris (dog)	ENPEP	XP_535696.2
Drosophila melanogaster (fruit fly)	CG8773	NP_650273.2
Drosophila melanogaster (fruit fly)	CG8774	NP_650274.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003077	XP_003436384.1
Bos taurus (cattle)	ENPEP	NP_001033116.1
Rattus norvegicus (Norway rat)	Enpep	NP_071587.2
Danio rerio (zebrafish)	enpep	NP_001186660.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG32473 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG32473 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04790	NM_142019.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG32473), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa04788	NM_169503.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG32473), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa04789	NM_169504.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG32473), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG32473 ( NM_142019.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650276.2 Drosophila melanogaster uncharacterized protein, transcript variant B (CG32473), mRNA.

Price & Availability↑

CloneID	OFa04790
Clone ID Related Accession (Same CDS sequence)	NM_142019.3
Accession Version	NM_142019.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_142019.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAACTG TGGCGCCCGC TGTAAAAGCC GCGAGCAAGG TGCTCCAGAA TCTGGGATTC 
CGGCTGCCCA AACAGATTAA GCCAAGCAAG TACCGCCTTC ATTTGCGCCC CGATCTCGAA 
CGAAAGAACT ATTCCGGCAA TATAAGCATC AGCCTGCAAG TACTGGAGCC CATAGCCTTC 
ATCCCAGTCC ATGTGAAGCA ACTGAATGTG AGCACCGTTG AGGTGAAGCG CCTGGATGAA 
TCTGGAGCGC CGCTTAAGGA CATCACCCCC ACGCTGACCT TTGCCCATCC GGAGTTTGAG 
TACTGGGTGA CCGAGTTCGA ACAGCCGCTG GAGGCGGGAA ACTACTCACT GCTGCTCAAC 
TTTACGGGAT CGCTTGTAGA TCGGATTACG GGGATGTACC AGAGCTCCTA CTTGGATAAG 
CTGAAAAACC GCTCCAGATC AATCATTTCC ACAAAGTTTG AGCCCACTTA TGCCCGCCAA 
GCATTCCCCT GCTTCGATGA GCCCGCCCTT AAGGCTCAGT TCACCATAAC GGTGGCCCGT 
CCAAGTGGTG ATGAGTACCA TGTGCTGTCC AACATGCCAG TGGCCAGCGA GTATGTCGAT 
GGCGATATCA CCGAGGTGAC TTTCGCGGAG ACCGTACCCA TGAGCACCTA TCTGGCCGCC 
TTTGTGGTGT CTGATTTTCA ATACAAGGAA ACCACTGTGG AGGGAACCAG CATCGCTCTG 
AAAGTCTATG CGCCACCAGC GCAGGTGGAG AAAACCCAGT ACGCCCTGGA CACTGCGGCC 
GGAGTGATGG CCTACTACAT CAACTATTTC AATGTTTCGT ATGCCCTACC CAAATTGGAT 
CTGGTTGCCA TACCCGATTT CGTGTCCGGT GCTATGGAAA ACTGGGGATT GGTCACGTTC 
CGGGAGACGG CTCTTCTGTA CGACGAATCT ACCAGTTCCA GCGTGAATAA GCAGCGTGTG 
GCCATCGTCG TGGCCCATGA GTTGGCTCAC CAGTGGTTCG GAAACCTGGT AACCATGAAC 
TGGTGGAACG ATTTGTGGCT AAACGAGGGT TTCGCATCAT TCCTTGAGTA CAAGGGAGTA 
AAGCAAATGC ATCCGGAATG GGATATGGAC AATCAGTTTG TTATTGAGGA ACTGCACCCG 
GTGCTGACCA TTGATGCCAC CTTAGCCTCC CATCCGATTG TCAAGTCCAT TGAGAGTCCA 
GCCGAGATCA CGGAGTACTT CGATACCATC ACCTATTCAA AGGGAGCCGC CTTAGTACGC 
ATGCTTGAGA ATCTGGTGGG CGAGGAAAAG TTGAGGAACG CCACCACCCG CTATTTGGTG 
CGTCATATCT ACAGTACTGC CACCACCGAG GATTACCTCA CCGCAGTCGA GGAGGAGGAG 
GGCCTGGAAT TTGACGTGAA GCAGATCATG CAGACCTGGA CGGAGCAGAT GGGTCTTCCG 
GTTGTGGAGG TGGAGAAAAG TGGCAGTACT TATAAGCTCA CCCAAAAGCG TTTCCTGGCC 
AACGAAGATG ACTACGCAGC CGAAGCTGAA GCATCGTCCT TCAACTACCG TTGGTCGATT 
CCCATTACCT ACACGAGCAG CATCAACAGT GAAGTCCAAT CACTAATATT CAACCACAAT 
GACAATGAGG CCACCATAAC GCTTCCGGAA GAGGCCAGCT GGATTAAGAT TAACACGAAT 
CAAGTGGGCT ACTACCGGGT GAACTACGGC AGCGAACAGT GGGCGGAACT CATATCGGCC 
CTGAAGAACT CCCGGGAAAC CTTCAGCACC GCCGATCGCG CTCATCTGCT GAACGATGCC 
AATACCCTGG CCGCTGCCGG ACAGCTTAAC TACTCCGTGG CACTGGATCT GATATCTTAC 
CTGGAGAGCG AGCAGGACTA CGTGCCCTGG AGCGTAGGCA CCTCCGCCCT GGCTACGTTA 
AGGAACCGCG TATACTACAC GGACTTGTAC ACTAATTACA CCACCTACGC CCGTAAACTG 
CTTACGCCCA TCGTAGAAAA GGTCACGTTT ACCGTGGCAG CCGATCATCT GGAAAATCGC 
CTCCGTATTA AGGTGCTGAG CTCAGCCTGC AGCTTGGGAC ATGAGAGCTC CTTGCAACAG 
GCGGTCACGC TCTTCAACCA GTGGCTAGCC AGCCCCGAGA CCCGGCCCAA TCCGGATATC 
CGAGACGTTG TCTACTACTA TGGTCTGCAA CAGGTGAACA CAGAGGCTGC CTGGGATCAA 
GTTTGGAAGC TGTATCTGGA CGAGAGTGAT GCCCAGGAAA AGCTGAAGTT GATGAACTGC 
CTAACCGCCG TGCAGGTGCC ATGGCTGTTG CAGCGTTACA TCAACTGGGC CTGGGATGAG 
AGCAATGTCC GCCGCCAGGA CTACTTCACC CTGCTTGGAT ATATATCCAC CAATCCGGTG 
GGTCAGAGTC TTGTGTGGGA TTACGTGCGT GAAAACTGGG AGAAGTTGGT GGATCGTTTC 
GGCATCAATG AACGCACCTT GGGTCGTCTG ATCCCCACGA TCACTGCCCG CTTCTCAACA 
GAAACCAAGC TGGAAGAGAT GCAGCAGTTC TTTGCCAAAT ATCCCGAGGC TGGAGCCGGA 
ACTGCGGCCC GTCAACAGGC GTTGGAAGCG GTGAAGGCAA ACATCAAATG GCTAGCGGCC 
AACAAGGCCC AGGTGGGCGA ATGGTTGGCC AATTACGTTC AACAGTCCAG CGTCACCAAC 
CGCATTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650276.2
CDS	134..2845
Translation	MATVAPAVKAASKVLQNLGFRLPKQIKPSKYRLHLRPDLERKNYSGNISISLQVLEPIAFIPVHVKQLNVSTVEVKRLDESGAPLKDITPTLTFAHPEFEYWVTEFEQPLEAGNYSLLLNFTGSLVDRITGMYQSSYLDKLKNRSRSIISTKFEPTYARQAFPCFDEPALKAQFTITVARPSGDEYHVLSNMPVASEYVDGDITEVTFAETVPMSTYLAAFVVSDFQYKETTVEGTSIALKVYAPPAQVEKTQYALDTAAGVMAYYINYFNVSYALPKLDLVAIPDFVSGAMENWGLVTFRETALLYDESTSSSVNKQRVAIVVAHELAHQWFGNLVTMNWWNDLWLNEGFASFLEYKGVKQMHPEWDMDNQFVIEELHPVLTIDATLASHPIVKSIESPAEITEYFDTITYSKGAALVRMLENLVGEEKLRNATTRYLVRHIYSTATTEDYLTAVEEEEGLEFDVKQIMQTWTEQMGLPVVEVEKSGSTYKLTQKRFLANEDDYAAEAEASSFNYRWSIPITYTSSINSEVQSLIFNHNDNEATITLPEEASWIKINTNQVGYYRVNYGSEQWAELISALKNSRETFSTADRAHLLNDANTLAAAGQLNYSVALDLISYLESEQDYVPWSVGTSALATLRNRVYYTDLYTNYTTYARKLLTPIVEKVTFTVAADHLENRLRIKVLSSACSLGHESSLQQAVTLFNQWLASPETRPNPDIRDVVYYYGLQQVNTEAAWDQVWKLYLDESDAQEKLKLMNCLTAVQVPWLLQRYINWAWDESNVRRQDYFTLLGYISTNPVGQSLVWDYVRENWEKLVDRFGINERTLGRLIPTITARFSTETKLEEMQQFFAKYPEAGAGTAARQQALEAVKANIKWLAANKAQVGEWLANYVQQSSVTNRIQ

Target ORF information:

RefSeq Version	NM_142019.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG32473), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142019.3

ORF Insert Sequence:

ATGGCAACTG TGGCGCCCGC TGTAAAAGCC GCGAGCAAGG TGCTCCAGAA TCTGGGATTC 
CGGCTGCCCA AACAGATTAA GCCAAGCAAG TACCGCCTTC ATTTGCGCCC CGATCTCGAA 
CGAAAGAACT ATTCCGGCAA TATAAGCATC AGCCTGCAAG TACTGGAGCC CATAGCCTTC 
ATCCCAGTCC ATGTGAAGCA ACTGAATGTG AGCACCGTTG AGGTGAAGCG CCTGGATGAA 
TCTGGAGCGC CGCTTAAGGA CATCACCCCC ACGCTGACCT TTGCCCATCC GGAGTTTGAG 
TACTGGGTGA CCGAGTTCGA ACAGCCGCTG GAGGCGGGAA ACTACTCACT GCTGCTCAAC 
TTTACGGGAT CGCTTGTAGA TCGGATTACG GGGATGTACC AGAGCTCCTA CTTGGATAAG 
CTGAAAAACC GCTCCAGATC AATCATTTCC ACAAAGTTTG AGCCCACTTA TGCCCGCCAA 
GCATTCCCCT GCTTCGATGA GCCCGCCCTT AAGGCTCAGT TCACCATAAC GGTGGCCCGT 
CCAAGTGGTG ATGAGTACCA TGTGCTGTCC AACATGCCAG TGGCCAGCGA GTATGTCGAT 
GGCGATATCA CCGAGGTGAC TTTCGCGGAG ACCGTACCCA TGAGCACCTA TCTGGCCGCC 
TTTGTGGTGT CTGATTTTCA ATACAAGGAA ACCACTGTGG AGGGAACCAG CATCGCTCTG 
AAAGTCTATG CGCCACCAGC GCAGGTGGAG AAAACCCAGT ACGCCCTGGA CACTGCGGCC 
GGAGTGATGG CCTACTACAT CAACTATTTC AATGTTTCGT ATGCCCTACC CAAATTGGAT 
CTGGTTGCCA TACCCGATTT CGTGTCCGGT GCTATGGAAA ACTGGGGATT GGTCACGTTC 
CGGGAGACGG CTCTTCTGTA CGACGAATCT ACCAGTTCCA GCGTGAATAA GCAGCGTGTG 
GCCATCGTCG TGGCCCATGA GTTGGCTCAC CAGTGGTTCG GAAACCTGGT AACCATGAAC 
TGGTGGAACG ATTTGTGGCT AAACGAGGGT TTCGCATCAT TCCTTGAGTA CAAGGGAGTA 
AAGCAAATGC ATCCGGAATG GGATATGGAC AATCAGTTTG TTATTGAGGA ACTGCACCCG 
GTGCTGACCA TTGATGCCAC CTTAGCCTCC CATCCGATTG TCAAGTCCAT TGAGAGTCCA 
GCCGAGATCA CGGAGTACTT CGATACCATC ACCTATTCAA AGGGAGCCGC CTTAGTACGC 
ATGCTTGAGA ATCTGGTGGG CGAGGAAAAG TTGAGGAACG CCACCACCCG CTATTTGGTG 
CGTCATATCT ACAGTACTGC CACCACCGAG GATTACCTCA CCGCAGTCGA GGAGGAGGAG 
GGCCTGGAAT TTGACGTGAA GCAGATCATG CAGACCTGGA CGGAGCAGAT GGGTCTTCCG 
GTTGTGGAGG TGGAGAAAAG TGGCAGTACT TATAAGCTCA CCCAAAAGCG TTTCCTGGCC 
AACGAAGATG ACTACGCAGC CGAAGCTGAA GCATCGTCCT TCAACTACCG TTGGTCGATT 
CCCATTACCT ACACGAGCAG CATCAACAGT GAAGTCCAAT CACTAATATT CAACCACAAT 
GACAATGAGG CCACCATAAC GCTTCCGGAA GAGGCCAGCT GGATTAAGAT TAACACGAAT 
CAAGTGGGCT ACTACCGGGT GAACTACGGC AGCGAACAGT GGGCGGAACT CATATCGGCC 
CTGAAGAACT CCCGGGAAAC CTTCAGCACC GCCGATCGCG CTCATCTGCT GAACGATGCC 
AATACCCTGG CCGCTGCCGG ACAGCTTAAC TACTCCGTGG CACTGGATCT GATATCTTAC 
CTGGAGAGCG AGCAGGACTA CGTGCCCTGG AGCGTAGGCA CCTCCGCCCT GGCTACGTTA 
AGGAACCGCG TATACTACAC GGACTTGTAC ACTAATTACA CCACCTACGC CCGTAAACTG 
CTTACGCCCA TCGTAGAAAA GGTCACGTTT ACCGTGGCAG CCGATCATCT GGAAAATCGC 
CTCCGTATTA AGGTGCTGAG CTCAGCCTGC AGCTTGGGAC ATGAGAGCTC CTTGCAACAG 
GCGGTCACGC TCTTCAACCA GTGGCTAGCC AGCCCCGAGA CCCGGCCCAA TCCGGATATC 
CGAGACGTTG TCTACTACTA TGGTCTGCAA CAGGTGAACA CAGAGGCTGC CTGGGATCAA 
GTTTGGAAGC TGTATCTGGA CGAGAGTGAT GCCCAGGAAA AGCTGAAGTT GATGAACTGC 
CTAACCGCCG TGCAGGTGCC ATGGCTGTTG CAGCGTTACA TCAACTGGGC CTGGGATGAG 
AGCAATGTCC GCCGCCAGGA CTACTTCACC CTGCTTGGAT ATATATCCAC CAATCCGGTG 
GGTCAGAGTC TTGTGTGGGA TTACGTGCGT GAAAACTGGG AGAAGTTGGT GGATCGTTTC 
GGCATCAATG AACGCACCTT GGGTCGTCTG ATCCCCACGA TCACTGCCCG CTTCTCAACA 
GAAACCAAGC TGGAAGAGAT GCAGCAGTTC TTTGCCAAAT ATCCCGAGGC TGGAGCCGGA 
ACTGCGGCCC GTCAACAGGC GTTGGAAGCG GTGAAGGCAA ACATCAAATG GCTAGCGGCC 
AACAAGGCCC AGGTGGGCGA ATGGTTGGCC AATTACGTTC AACAGTCCAG CGTCACCAAC 
CGCATTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG32473 ( NM_169503.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731786.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG32473), mRNA.

Price & Availability↑

CloneID	OFa04788
Clone ID Related Accession (Same CDS sequence)	NM_169503.3
Accession Version	NM_169503.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169503.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTGCCT GGCTGACGGT TAAGTCAGTG GCCATCTTTT TGGGCCTGGC CTCTACTGCT 
TTTTGTGTGG CCACGATTGT GCTGGCCGTT CAGAATGCGG ACCTGGAGAA CGATCTGCAG 
GATGCCCTCG ATAAGATCGA CGCATTGACG GTGGACACCA CGTCCACGTC CTCCACCTCT 
ACCAGCACTT CCACGACCAC CACCACTGCA AATCCCAATG GGCCCACGGA TGACACCGAG 
CCTGGCTCTA CCACCACACC TGGCGTAGGA GGTGACACAA CCCCCAGTGC AGGAACATCT 
CCGGGTGGTG AAACTTCGCC ATCATCCAGT ACAGGAAGCA CCACCAATCC CATCTATCCG 
ACATTGCCGA CAGGACTTCC TGATCCTGAA AAGATCGAAT GGCGCCTGCC CACGGAACTG 
ACGCCCATCA AGTACAAGGT GTACTACCAT CCCGACTTGA CGACCGGCGC CTGTGAGGGA 
ACCGTGTCCA TTCAGTTCCA GCTGAACGCG ATCACCAACC TGATCGTCCT GCACGCCAAG 
GAGCTCAATG TGCACAGCAT CAGCATCCTG AACATGATGG CGCGCATACG CGTGGCCATC 
GATAGCATCA ACTTGGATGA GTCCCGGGAG CTGCTCCTGA TCACGCTAAG AGAGGTCCTC 
AGTATGAACA AGGCCTACAC ACTGTCCGCA AGTTTCGACT GCGATCTGAG CAGTCTGGTG 
GGCAGCTACA TCAGCAACTA CACGAATGCC GATGGAGTAG ATAGATCAAT CATTTCCACA 
AAGTTTGAGC CCACTTATGC CCGCCAAGCA TTCCCCTGCT TCGATGAGCC CGCCCTTAAG 
GCTCAGTTCA CCATAACGGT GGCCCGTCCA AGTGGTGATG AGTACCATGT GCTGTCCAAC 
ATGCCAGTGG CCAGCGAGTA TGTCGATGGC GATATCACCG AGGTGACTTT CGCGGAGACC 
GTACCCATGA GCACCTATCT GGCCGCCTTT GTGGTGTCTG ATTTTCAATA CAAGGAAACC 
ACTGTGGAGG GAACCAGCAT CGCTCTGAAA GTCTATGCGC CACCAGCGCA GGTGGAGAAA 
ACCCAGTACG CCCTGGACAC TGCGGCCGGA GTGATGGCCT ACTACATCAA CTATTTCAAT 
GTTTCGTATG CCCTACCCAA ATTGGATCTG GTTGCCATAC CCGATTTCGT GTCCGGTGCT 
ATGGAAAACT GGGGATTGGT CACGTTCCGG GAGACGGCTC TTCTGTACGA CGAATCTACC 
AGTTCCAGCG TGAATAAGCA GCGTGTGGCC ATCGTCGTGG CCCATGAGTT GGCTCACCAG 
TGGTTCGGAA ACCTGGTAAC CATGAACTGG TGGAACGATT TGTGGCTAAA CGAGGGTTTC 
GCATCATTCC TTGAGTACAA GGGAGTAAAG CAAATGCATC CGGAATGGGA TATGGACAAT 
CAGTTTGTTA TTGAGGAACT GCACCCGGTG CTGACCATTG ATGCCACCTT AGCCTCCCAT 
CCGATTGTCA AGTCCATTGA GAGTCCAGCC GAGATCACGG AGTACTTCGA TACCATCACC 
TATTCAAAGG GAGCCGCCTT AGTACGCATG CTTGAGAATC TGGTGGGCGA GGAAAAGTTG 
AGGAACGCCA CCACCCGCTA TTTGGTGCGT CATATCTACA GTACTGCCAC CACCGAGGAT 
TACCTCACCG CAGTCGAGGA GGAGGAGGGC CTGGAATTTG ACGTGAAGCA GATCATGCAG 
ACCTGGACGG AGCAGATGGG TCTTCCGGTT GTGGAGGTGG AGAAAAGTGG CAGTACTTAT 
AAGCTCACCC AAAAGCGTTT CCTGGCCAAC GAAGATGACT ACGCAGCCGA AGCTGAAGCA 
TCGTCCTTCA ACTACCGTTG GTCGATTCCC ATTACCTACA CGAGCAGCAT CAACAGTGAA 
GTCCAATCAC TAATATTCAA CCACAATGAC AATGAGGCCA CCATAACGCT TCCGGAAGAG 
GCCAGCTGGA TTAAGATTAA CACGAATCAA GTGGGCTACT ACCGGGTGAA CTACGGCAGC 
GAACAGTGGG CGGAACTCAT ATCGGCCCTG AAGAACTCCC GGGAAACCTT CAGCACCGCC 
GATCGCGCTC ATCTGCTGAA CGATGCCAAT ACCCTGGCCG CTGCCGGACA GCTTAACTAC 
TCCGTGGCAC TGGATCTGAT ATCTTACCTG GAGAGCGAGC AGGACTACGT GCCCTGGAGC 
GTAGGCACCT CCGCCCTGGC TACGTTAAGG AACCGCGTAT ACTACACGGA CTTGTACACT 
AATTACACCA CCTACGCCCG TAAACTGCTT ACGCCCATCG TAGAAAAGGT CACGTTTACC 
GTGGCAGCCG ATCATCTGGA AAATCGCCTC CGTATTAAGG TGCTGAGCTC AGCCTGCAGC 
TTGGGACATG AGAGCTCCTT GCAACAGGCG GTCACGCTCT TCAACCAGTG GCTAGCCAGC 
CCCGAGACCC GGCCCAATCC GGATATCCGA GACGTTGTCT ACTACTATGG TCTGCAACAG 
GTGAACACAG AGGCTGCCTG GGATCAAGTT TGGAAGCTGT ATCTGGACGA GAGTGATGCC 
CAGGAAAAGC TGAAGTTGAT GAACTGCCTA ACCGCCGTGC AGGTGCCATG GCTGTTGCAG 
CGTTACATCA ACTGGGCCTG GGATGAGAGC AATGTCCGCC GCCAGGACTA CTTCACCCTG 
CTTGGATATA TATCCACCAA TCCGGTGGGT CAGAGTCTTG TGTGGGATTA CGTGCGTGAA 
AACTGGGAGA AGTTGGTGGA TCGTTTCGGC ATCAATGAAC GCACCTTGGG TCGTCTGATC 
CCCACGATCA CTGCCCGCTT CTCAACAGAA ACCAAGCTGG AAGAGATGCA GCAGTTCTTT 
GCCAAATATC CCGAGGCTGG AGCCGGAACT GCGGCCCGTC AACAGGCGTT GGAAGCGGTG 
AAGGCAAACA TCAAATGGCT AGCGGCCAAC AAGGCCCAGG TGGGCGAATG GTTGGCCAAT 
TACGTTCAAC AGTCCAGCGT CACCAACCGC ATTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731786.1
CDS	78..3116
Translation	MVAWLTVKSVAIFLGLASTAFCVATIVLAVQNADLENDLQDALDKIDALTVDTTSTSSTSTSTSTTTTTANPNGPTDDTEPGSTTTPGVGGDTTPSAGTSPGGETSPSSSTGSTTNPIYPTLPTGLPDPEKIEWRLPTELTPIKYKVYYHPDLTTGACEGTVSIQFQLNAITNLIVLHAKELNVHSISILNMMARIRVAIDSINLDESRELLLITLREVLSMNKAYTLSASFDCDLSSLVGSYISNYTNADGVDRSIISTKFEPTYARQAFPCFDEPALKAQFTITVARPSGDEYHVLSNMPVASEYVDGDITEVTFAETVPMSTYLAAFVVSDFQYKETTVEGTSIALKVYAPPAQVEKTQYALDTAAGVMAYYINYFNVSYALPKLDLVAIPDFVSGAMENWGLVTFRETALLYDESTSSSVNKQRVAIVVAHELAHQWFGNLVTMNWWNDLWLNEGFASFLEYKGVKQMHPEWDMDNQFVIEELHPVLTIDATLASHPIVKSIESPAEITEYFDTITYSKGAALVRMLENLVGEEKLRNATTRYLVRHIYSTATTEDYLTAVEEEEGLEFDVKQIMQTWTEQMGLPVVEVEKSGSTYKLTQKRFLANEDDYAAEAEASSFNYRWSIPITYTSSINSEVQSLIFNHNDNEATITLPEEASWIKINTNQVGYYRVNYGSEQWAELISALKNSRETFSTADRAHLLNDANTLAAAGQLNYSVALDLISYLESEQDYVPWSVGTSALATLRNRVYYTDLYTNYTTYARKLLTPIVEKVTFTVAADHLENRLRIKVLSSACSLGHESSLQQAVTLFNQWLASPETRPNPDIRDVVYYYGLQQVNTEAAWDQVWKLYLDESDAQEKLKLMNCLTAVQVPWLLQRYINWAWDESNVRRQDYFTLLGYISTNPVGQSLVWDYVRENWEKLVDRFGINERTLGRLIPTITARFSTETKLEEMQQFFAKYPEAGAGTAARQQALEAVKANIKWLAANKAQVGEWLANYVQQSSVTNRIQ

Target ORF information:

RefSeq Version	NM_169503.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG32473), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169503.3

ORF Insert Sequence:

ATGGTTGCCT GGCTGACGGT TAAGTCAGTG GCCATCTTTT TGGGCCTGGC CTCTACTGCT 
TTTTGTGTGG CCACGATTGT GCTGGCCGTT CAGAATGCGG ACCTGGAGAA CGATCTGCAG 
GATGCCCTCG ATAAGATCGA CGCATTGACG GTGGACACCA CGTCCACGTC CTCCACCTCT 
ACCAGCACTT CCACGACCAC CACCACTGCA AATCCCAATG GGCCCACGGA TGACACCGAG 
CCTGGCTCTA CCACCACACC TGGCGTAGGA GGTGACACAA CCCCCAGTGC AGGAACATCT 
CCGGGTGGTG AAACTTCGCC ATCATCCAGT ACAGGAAGCA CCACCAATCC CATCTATCCG 
ACATTGCCGA CAGGACTTCC TGATCCTGAA AAGATCGAAT GGCGCCTGCC CACGGAACTG 
ACGCCCATCA AGTACAAGGT GTACTACCAT CCCGACTTGA CGACCGGCGC CTGTGAGGGA 
ACCGTGTCCA TTCAGTTCCA GCTGAACGCG ATCACCAACC TGATCGTCCT GCACGCCAAG 
GAGCTCAATG TGCACAGCAT CAGCATCCTG AACATGATGG CGCGCATACG CGTGGCCATC 
GATAGCATCA ACTTGGATGA GTCCCGGGAG CTGCTCCTGA TCACGCTAAG AGAGGTCCTC 
AGTATGAACA AGGCCTACAC ACTGTCCGCA AGTTTCGACT GCGATCTGAG CAGTCTGGTG 
GGCAGCTACA TCAGCAACTA CACGAATGCC GATGGAGTAG ATAGATCAAT CATTTCCACA 
AAGTTTGAGC CCACTTATGC CCGCCAAGCA TTCCCCTGCT TCGATGAGCC CGCCCTTAAG 
GCTCAGTTCA CCATAACGGT GGCCCGTCCA AGTGGTGATG AGTACCATGT GCTGTCCAAC 
ATGCCAGTGG CCAGCGAGTA TGTCGATGGC GATATCACCG AGGTGACTTT CGCGGAGACC 
GTACCCATGA GCACCTATCT GGCCGCCTTT GTGGTGTCTG ATTTTCAATA CAAGGAAACC 
ACTGTGGAGG GAACCAGCAT CGCTCTGAAA GTCTATGCGC CACCAGCGCA GGTGGAGAAA 
ACCCAGTACG CCCTGGACAC TGCGGCCGGA GTGATGGCCT ACTACATCAA CTATTTCAAT 
GTTTCGTATG CCCTACCCAA ATTGGATCTG GTTGCCATAC CCGATTTCGT GTCCGGTGCT 
ATGGAAAACT GGGGATTGGT CACGTTCCGG GAGACGGCTC TTCTGTACGA CGAATCTACC 
AGTTCCAGCG TGAATAAGCA GCGTGTGGCC ATCGTCGTGG CCCATGAGTT GGCTCACCAG 
TGGTTCGGAA ACCTGGTAAC CATGAACTGG TGGAACGATT TGTGGCTAAA CGAGGGTTTC 
GCATCATTCC TTGAGTACAA GGGAGTAAAG CAAATGCATC CGGAATGGGA TATGGACAAT 
CAGTTTGTTA TTGAGGAACT GCACCCGGTG CTGACCATTG ATGCCACCTT AGCCTCCCAT 
CCGATTGTCA AGTCCATTGA GAGTCCAGCC GAGATCACGG AGTACTTCGA TACCATCACC 
TATTCAAAGG GAGCCGCCTT AGTACGCATG CTTGAGAATC TGGTGGGCGA GGAAAAGTTG 
AGGAACGCCA CCACCCGCTA TTTGGTGCGT CATATCTACA GTACTGCCAC CACCGAGGAT 
TACCTCACCG CAGTCGAGGA GGAGGAGGGC CTGGAATTTG ACGTGAAGCA GATCATGCAG 
ACCTGGACGG AGCAGATGGG TCTTCCGGTT GTGGAGGTGG AGAAAAGTGG CAGTACTTAT 
AAGCTCACCC AAAAGCGTTT CCTGGCCAAC GAAGATGACT ACGCAGCCGA AGCTGAAGCA 
TCGTCCTTCA ACTACCGTTG GTCGATTCCC ATTACCTACA CGAGCAGCAT CAACAGTGAA 
GTCCAATCAC TAATATTCAA CCACAATGAC AATGAGGCCA CCATAACGCT TCCGGAAGAG 
GCCAGCTGGA TTAAGATTAA CACGAATCAA GTGGGCTACT ACCGGGTGAA CTACGGCAGC 
GAACAGTGGG CGGAACTCAT ATCGGCCCTG AAGAACTCCC GGGAAACCTT CAGCACCGCC 
GATCGCGCTC ATCTGCTGAA CGATGCCAAT ACCCTGGCCG CTGCCGGACA GCTTAACTAC 
TCCGTGGCAC TGGATCTGAT ATCTTACCTG GAGAGCGAGC AGGACTACGT GCCCTGGAGC 
GTAGGCACCT CCGCCCTGGC TACGTTAAGG AACCGCGTAT ACTACACGGA CTTGTACACT 
AATTACACCA CCTACGCCCG TAAACTGCTT ACGCCCATCG TAGAAAAGGT CACGTTTACC 
GTGGCAGCCG ATCATCTGGA AAATCGCCTC CGTATTAAGG TGCTGAGCTC AGCCTGCAGC 
TTGGGACATG AGAGCTCCTT GCAACAGGCG GTCACGCTCT TCAACCAGTG GCTAGCCAGC 
CCCGAGACCC GGCCCAATCC GGATATCCGA GACGTTGTCT ACTACTATGG TCTGCAACAG 
GTGAACACAG AGGCTGCCTG GGATCAAGTT TGGAAGCTGT ATCTGGACGA GAGTGATGCC 
CAGGAAAAGC TGAAGTTGAT GAACTGCCTA ACCGCCGTGC AGGTGCCATG GCTGTTGCAG 
CGTTACATCA ACTGGGCCTG GGATGAGAGC AATGTCCGCC GCCAGGACTA CTTCACCCTG 
CTTGGATATA TATCCACCAA TCCGGTGGGT CAGAGTCTTG TGTGGGATTA CGTGCGTGAA 
AACTGGGAGA AGTTGGTGGA TCGTTTCGGC ATCAATGAAC GCACCTTGGG TCGTCTGATC 
CCCACGATCA CTGCCCGCTT CTCAACAGAA ACCAAGCTGG AAGAGATGCA GCAGTTCTTT 
GCCAAATATC CCGAGGCTGG AGCCGGAACT GCGGCCCGTC AACAGGCGTT GGAAGCGGTG 
AAGGCAAACA TCAAATGGCT AGCGGCCAAC AAGGCCCAGG TGGGCGAATG GTTGGCCAAT 
TACGTTCAAC AGTCCAGCGT CACCAACCGC ATTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG32473 ( NM_169504.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731787.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG32473), mRNA.

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CloneID	OFa04789
Clone ID Related Accession (Same CDS sequence)	NM_169504.3
Accession Version	NM_169504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169504.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATGCAG CGAAAAACGT ACGCCCGCGC AGCAGTCTCT CCACAGACGA CAATCACCGG 
ATCAAGGACA CCAATCGGCC CCACAAGGCC CGCAGCAACA CGATCCTGAC CCTCGCCGCA 
TTCTGCGTCT TTCTGCTAGT CCTCTGCGGC TTCCTGCTGG GCGCCCTCGT CTACGTCGGC 
AAGAATATAG CCGAGGAGCA CCAGAGGCAG CAGCACGCGA ATGCTTCCCA GTGGGCCTTG 
AACTCCACCA TCCGCACAGT TTACGTGGAT CGGGACCAGT TGCTATCCAC CAAGCCCATT 
ATCAGCGTAC TTTCAGATAA TCGCTTTTAC ACGACTCAAG TGCCAACCAG CAAAAAATCG 
AGCATAATGG CAACTGTGGC GCCCGCTGTA AAAGCCGCGA GCAAGGTGCT CCAGAATCTG 
GGATTCCGGC TGCCCAAACA GATTAAGCCA AGCAAGTACC GCCTTCATTT GCGCCCCGAT 
CTCGAACGAA AGAACTATTC CGGCAATATA AGCATCAGCC TGCAAGTACT GGAGCCCATA 
GCCTTCATCC CAGTCCATGT GAAGCAACTG AATGTGAGCA CCGTTGAGGT GAAGCGCCTG 
GATGAATCTG GAGCGCCGCT TAAGGACATC ACCCCCACGC TGACCTTTGC CCATCCGGAG 
TTTGAGTACT GGGTGACCGA GTTCGAACAG CCGCTGGAGG CGGGAAACTA CTCACTGCTG 
CTCAACTTTA CGGGATCGCT TGTAGATCGG ATTACGGGGA TGTACCAGAG CTCCTACTTG 
GATAAGCTGA AAAACCGCTC CAGATCAATC ATTTCCACAA AGTTTGAGCC CACTTATGCC 
CGCCAAGCAT TCCCCTGCTT CGATGAGCCC GCCCTTAAGG CTCAGTTCAC CATAACGGTG 
GCCCGTCCAA GTGGTGATGA GTACCATGTG CTGTCCAACA TGCCAGTGGC CAGCGAGTAT 
GTCGATGGCG ATATCACCGA GGTGACTTTC GCGGAGACCG TACCCATGAG CACCTATCTG 
GCCGCCTTTG TGGTGTCTGA TTTTCAATAC AAGGAAACCA CTGTGGAGGG AACCAGCATC 
GCTCTGAAAG TCTATGCGCC ACCAGCGCAG GTGGAGAAAA CCCAGTACGC CCTGGACACT 
GCGGCCGGAG TGATGGCCTA CTACATCAAC TATTTCAATG TTTCGTATGC CCTACCCAAA 
TTGGATCTGG TTGCCATACC CGATTTCGTG TCCGGTGCTA TGGAAAACTG GGGATTGGTC 
ACGTTCCGGG AGACGGCTCT TCTGTACGAC GAATCTACCA GTTCCAGCGT GAATAAGCAG 
CGTGTGGCCA TCGTCGTGGC CCATGAGTTG GCTCACCAGT GGTTCGGAAA CCTGGTAACC 
ATGAACTGGT GGAACGATTT GTGGCTAAAC GAGGGTTTCG CATCATTCCT TGAGTACAAG 
GGAGTAAAGC AAATGCATCC GGAATGGGAT ATGGACAATC AGTTTGTTAT TGAGGAACTG 
CACCCGGTGC TGACCATTGA TGCCACCTTA GCCTCCCATC CGATTGTCAA GTCCATTGAG 
AGTCCAGCCG AGATCACGGA GTACTTCGAT ACCATCACCT ATTCAAAGGG AGCCGCCTTA 
GTACGCATGC TTGAGAATCT GGTGGGCGAG GAAAAGTTGA GGAACGCCAC CACCCGCTAT 
TTGGTGCGTC ATATCTACAG TACTGCCACC ACCGAGGATT ACCTCACCGC AGTCGAGGAG 
GAGGAGGGCC TGGAATTTGA CGTGAAGCAG ATCATGCAGA CCTGGACGGA GCAGATGGGT 
CTTCCGGTTG TGGAGGTGGA GAAAAGTGGC AGTACTTATA AGCTCACCCA AAAGCGTTTC 
CTGGCCAACG AAGATGACTA CGCAGCCGAA GCTGAAGCAT CGTCCTTCAA CTACCGTTGG 
TCGATTCCCA TTACCTACAC GAGCAGCATC AACAGTGAAG TCCAATCACT AATATTCAAC 
CACAATGACA ATGAGGCCAC CATAACGCTT CCGGAAGAGG CCAGCTGGAT TAAGATTAAC 
ACGAATCAAG TGGGCTACTA CCGGGTGAAC TACGGCAGCG AACAGTGGGC GGAACTCATA 
TCGGCCCTGA AGAACTCCCG GGAAACCTTC AGCACCGCCG ATCGCGCTCA TCTGCTGAAC 
GATGCCAATA CCCTGGCCGC TGCCGGACAG CTTAACTACT CCGTGGCACT GGATCTGATA 
TCTTACCTGG AGAGCGAGCA GGACTACGTG CCCTGGAGCG TAGGCACCTC CGCCCTGGCT 
ACGTTAAGGA ACCGCGTATA CTACACGGAC TTGTACACTA ATTACACCAC CTACGCCCGT 
AAACTGCTTA CGCCCATCGT AGAAAAGGTC ACGTTTACCG TGGCAGCCGA TCATCTGGAA 
AATCGCCTCC GTATTAAGGT GCTGAGCTCA GCCTGCAGCT TGGGACATGA GAGCTCCTTG 
CAACAGGCGG TCACGCTCTT CAACCAGTGG CTAGCCAGCC CCGAGACCCG GCCCAATCCG 
GATATCCGAG ACGTTGTCTA CTACTATGGT CTGCAACAGG TGAACACAGA GGCTGCCTGG 
GATCAAGTTT GGAAGCTGTA TCTGGACGAG AGTGATGCCC AGGAAAAGCT GAAGTTGATG 
AACTGCCTAA CCGCCGTGCA GGTGCCATGG CTGTTGCAGC GTTACATCAA CTGGGCCTGG 
GATGAGAGCA ATGTCCGCCG CCAGGACTAC TTCACCCTGC TTGGATATAT ATCCACCAAT 
CCGGTGGGTC AGAGTCTTGT GTGGGATTAC GTGCGTGAAA ACTGGGAGAA GTTGGTGGAT 
CGTTTCGGCA TCAATGAACG CACCTTGGGT CGTCTGATCC CCACGATCAC TGCCCGCTTC 
TCAACAGAAA CCAAGCTGGA AGAGATGCAG CAGTTCTTTG CCAAATATCC CGAGGCTGGA 
GCCGGAACTG CGGCCCGTCA ACAGGCGTTG GAAGCGGTGA AGGCAAACAT CAAATGGCTA 
GCGGCCAACA AGGCCCAGGT GGGCGAATGG TTGGCCAATT ACGTTCAACA GTCCAGCGTC 
ACCAACCGCA TTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731787.1
CDS	401..3478
Translation	MYAAKNVRPRSSLSTDDNHRIKDTNRPHKARSNTILTLAAFCVFLLVLCGFLLGALVYVGKNIAEEHQRQQHANASQWALNSTIRTVYVDRDQLLSTKPIISVLSDNRFYTTQVPTSKKSSIMATVAPAVKAASKVLQNLGFRLPKQIKPSKYRLHLRPDLERKNYSGNISISLQVLEPIAFIPVHVKQLNVSTVEVKRLDESGAPLKDITPTLTFAHPEFEYWVTEFEQPLEAGNYSLLLNFTGSLVDRITGMYQSSYLDKLKNRSRSIISTKFEPTYARQAFPCFDEPALKAQFTITVARPSGDEYHVLSNMPVASEYVDGDITEVTFAETVPMSTYLAAFVVSDFQYKETTVEGTSIALKVYAPPAQVEKTQYALDTAAGVMAYYINYFNVSYALPKLDLVAIPDFVSGAMENWGLVTFRETALLYDESTSSSVNKQRVAIVVAHELAHQWFGNLVTMNWWNDLWLNEGFASFLEYKGVKQMHPEWDMDNQFVIEELHPVLTIDATLASHPIVKSIESPAEITEYFDTITYSKGAALVRMLENLVGEEKLRNATTRYLVRHIYSTATTEDYLTAVEEEEGLEFDVKQIMQTWTEQMGLPVVEVEKSGSTYKLTQKRFLANEDDYAAEAEASSFNYRWSIPITYTSSINSEVQSLIFNHNDNEATITLPEEASWIKINTNQVGYYRVNYGSEQWAELISALKNSRETFSTADRAHLLNDANTLAAAGQLNYSVALDLISYLESEQDYVPWSVGTSALATLRNRVYYTDLYTNYTTYARKLLTPIVEKVTFTVAADHLENRLRIKVLSSACSLGHESSLQQAVTLFNQWLASPETRPNPDIRDVVYYYGLQQVNTEAAWDQVWKLYLDESDAQEKLKLMNCLTAVQVPWLLQRYINWAWDESNVRRQDYFTLLGYISTNPVGQSLVWDYVRENWEKLVDRFGINERTLGRLIPTITARFSTETKLEEMQQFFAKYPEAGAGTAARQQALEAVKANIKWLAANKAQVGEWLANYVQQSSVTNRIQ

Target ORF information:

RefSeq Version	NM_169504.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG32473), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169504.3

ORF Insert Sequence:

ATGTATGCAG CGAAAAACGT ACGCCCGCGC AGCAGTCTCT CCACAGACGA CAATCACCGG 
ATCAAGGACA CCAATCGGCC CCACAAGGCC CGCAGCAACA CGATCCTGAC CCTCGCCGCA 
TTCTGCGTCT TTCTGCTAGT CCTCTGCGGC TTCCTGCTGG GCGCCCTCGT CTACGTCGGC 
AAGAATATAG CCGAGGAGCA CCAGAGGCAG CAGCACGCGA ATGCTTCCCA GTGGGCCTTG 
AACTCCACCA TCCGCACAGT TTACGTGGAT CGGGACCAGT TGCTATCCAC CAAGCCCATT 
ATCAGCGTAC TTTCAGATAA TCGCTTTTAC ACGACTCAAG TGCCAACCAG CAAAAAATCG 
AGCATAATGG CAACTGTGGC GCCCGCTGTA AAAGCCGCGA GCAAGGTGCT CCAGAATCTG 
GGATTCCGGC TGCCCAAACA GATTAAGCCA AGCAAGTACC GCCTTCATTT GCGCCCCGAT 
CTCGAACGAA AGAACTATTC CGGCAATATA AGCATCAGCC TGCAAGTACT GGAGCCCATA 
GCCTTCATCC CAGTCCATGT GAAGCAACTG AATGTGAGCA CCGTTGAGGT GAAGCGCCTG 
GATGAATCTG GAGCGCCGCT TAAGGACATC ACCCCCACGC TGACCTTTGC CCATCCGGAG 
TTTGAGTACT GGGTGACCGA GTTCGAACAG CCGCTGGAGG CGGGAAACTA CTCACTGCTG 
CTCAACTTTA CGGGATCGCT TGTAGATCGG ATTACGGGGA TGTACCAGAG CTCCTACTTG 
GATAAGCTGA AAAACCGCTC CAGATCAATC ATTTCCACAA AGTTTGAGCC CACTTATGCC 
CGCCAAGCAT TCCCCTGCTT CGATGAGCCC GCCCTTAAGG CTCAGTTCAC CATAACGGTG 
GCCCGTCCAA GTGGTGATGA GTACCATGTG CTGTCCAACA TGCCAGTGGC CAGCGAGTAT 
GTCGATGGCG ATATCACCGA GGTGACTTTC GCGGAGACCG TACCCATGAG CACCTATCTG 
GCCGCCTTTG TGGTGTCTGA TTTTCAATAC AAGGAAACCA CTGTGGAGGG AACCAGCATC 
GCTCTGAAAG TCTATGCGCC ACCAGCGCAG GTGGAGAAAA CCCAGTACGC CCTGGACACT 
GCGGCCGGAG TGATGGCCTA CTACATCAAC TATTTCAATG TTTCGTATGC CCTACCCAAA 
TTGGATCTGG TTGCCATACC CGATTTCGTG TCCGGTGCTA TGGAAAACTG GGGATTGGTC 
ACGTTCCGGG AGACGGCTCT TCTGTACGAC GAATCTACCA GTTCCAGCGT GAATAAGCAG 
CGTGTGGCCA TCGTCGTGGC CCATGAGTTG GCTCACCAGT GGTTCGGAAA CCTGGTAACC 
ATGAACTGGT GGAACGATTT GTGGCTAAAC GAGGGTTTCG CATCATTCCT TGAGTACAAG 
GGAGTAAAGC AAATGCATCC GGAATGGGAT ATGGACAATC AGTTTGTTAT TGAGGAACTG 
CACCCGGTGC TGACCATTGA TGCCACCTTA GCCTCCCATC CGATTGTCAA GTCCATTGAG 
AGTCCAGCCG AGATCACGGA GTACTTCGAT ACCATCACCT ATTCAAAGGG AGCCGCCTTA 
GTACGCATGC TTGAGAATCT GGTGGGCGAG GAAAAGTTGA GGAACGCCAC CACCCGCTAT 
TTGGTGCGTC ATATCTACAG TACTGCCACC ACCGAGGATT ACCTCACCGC AGTCGAGGAG 
GAGGAGGGCC TGGAATTTGA CGTGAAGCAG ATCATGCAGA CCTGGACGGA GCAGATGGGT 
CTTCCGGTTG TGGAGGTGGA GAAAAGTGGC AGTACTTATA AGCTCACCCA AAAGCGTTTC 
CTGGCCAACG AAGATGACTA CGCAGCCGAA GCTGAAGCAT CGTCCTTCAA CTACCGTTGG 
TCGATTCCCA TTACCTACAC GAGCAGCATC AACAGTGAAG TCCAATCACT AATATTCAAC 
CACAATGACA ATGAGGCCAC CATAACGCTT CCGGAAGAGG CCAGCTGGAT TAAGATTAAC 
ACGAATCAAG TGGGCTACTA CCGGGTGAAC TACGGCAGCG AACAGTGGGC GGAACTCATA 
TCGGCCCTGA AGAACTCCCG GGAAACCTTC AGCACCGCCG ATCGCGCTCA TCTGCTGAAC 
GATGCCAATA CCCTGGCCGC TGCCGGACAG CTTAACTACT CCGTGGCACT GGATCTGATA 
TCTTACCTGG AGAGCGAGCA GGACTACGTG CCCTGGAGCG TAGGCACCTC CGCCCTGGCT 
ACGTTAAGGA ACCGCGTATA CTACACGGAC TTGTACACTA ATTACACCAC CTACGCCCGT 
AAACTGCTTA CGCCCATCGT AGAAAAGGTC ACGTTTACCG TGGCAGCCGA TCATCTGGAA 
AATCGCCTCC GTATTAAGGT GCTGAGCTCA GCCTGCAGCT TGGGACATGA GAGCTCCTTG 
CAACAGGCGG TCACGCTCTT CAACCAGTGG CTAGCCAGCC CCGAGACCCG GCCCAATCCG 
GATATCCGAG ACGTTGTCTA CTACTATGGT CTGCAACAGG TGAACACAGA GGCTGCCTGG 
GATCAAGTTT GGAAGCTGTA TCTGGACGAG AGTGATGCCC AGGAAAAGCT GAAGTTGATG 
AACTGCCTAA CCGCCGTGCA GGTGCCATGG CTGTTGCAGC GTTACATCAA CTGGGCCTGG 
GATGAGAGCA ATGTCCGCCG CCAGGACTAC TTCACCCTGC TTGGATATAT ATCCACCAAT 
CCGGTGGGTC AGAGTCTTGT GTGGGATTAC GTGCGTGAAA ACTGGGAGAA GTTGGTGGAT 
CGTTTCGGCA TCAATGAACG CACCTTGGGT CGTCTGATCC CCACGATCAC TGCCCGCTTC 
TCAACAGAAA CCAAGCTGGA AGAGATGCAG CAGTTCTTTG CCAAATATCC CGAGGCTGGA 
GCCGGAACTG CGGCCCGTCA ACAGGCGTTG GAAGCGGTGA AGGCAAACAT CAAATGGCTA 
GCGGCCAACA AGGCCCAGGT GGGCGAATGG TTGGCCAATT ACGTTCAACA GTCCAGCGTC 
ACCAACCGCA TTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG32473 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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