wntD cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wntD
Entrez Gene ID	41633
Full Name	wnt inhibitor of Dorsal
Synonyms	CG8458,DWnt-8,DWnt8,Dm DWnt8,Dmel\CG8458,Wnt8,WntD,wnt8
General protein information	Preferred Names wnt inhibitor of Dorsal Names CG8458 gene product from transcript CG8458-RA CG8458-PA wnt inhibitor of dorsal wntD-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87E4-87E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_142015.2	NP_650272.1	wnt inhibitor of dorsal

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study established that WntD and Die are 2 cytokines that regulate independently the expression of edin during infection by an intracellular bacteria and a virus, respectively.
Title: WntD and Diedel: Two immunomodulatory cytokines in Drosophila immunity.

WntD accumulates until the Toll gradient narrows to its desired spread
Title: A WntD-Dependent Integral Feedback Loop Attenuates Variability in Drosophila Toll Signaling.

Crystal structure of the wntD indicate conformational plasticity in the N-terminal domain.
Title: structural Studies of Wnts and identification of an LRP6 binding site.

WntD, a recently characterized Drosophila Wnt family member, does not require Porcupine or Wntless/Evi/Sprinter for its secretion or signaling activity
Title: Lipid-independent secretion of a Drosophila Wnt protein.

WntD acts as a feedback inhibitor of the NF-kappaB homologue Dorsal during both embryonic patterning and the innate immune response to infection
Title: WntD is a feedback inhibitor of Dorsal/NF-kappaB in Drosophila development and immunity.

The following wntD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wntD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04784	NM_142015.2 Latest version!	Drosophila melanogaster wnt inhibitor of dorsal (wntD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wntD ( NM_142015.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650272.1 Drosophila melanogaster wnt inhibitor of dorsal (wntD), mRNA.

Price & Availability↑

CloneID	OFa04784
Clone ID Related Accession (Same CDS sequence)	NM_142015.2
Accession Version	NM_142015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wnt inhibitor of dorsal
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Nov 6, 2002 this sequence version replaced NM_142015.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTTTTG CCATCACATT CTTCATGGGT ATTACGAGCA CTCTGGCAGC AGTTTTGGAG 
CCCATGAGCT ACTACCAGTA CACCCAGTTC CAGGCACCAC TCTCCTGGGA GGATATAACC 
GGCAAGGGGC TGAAACAGGC CCTCGACAGC TGCCAACAGA GTTTCCAGTG GCAGCGATGG 
AACTGTCCGA GCCAGGATTT CGTGCAGAAG AATTCCAAGC CGGAGGAAAA CTCACCAAAT 
CGGGAGGATG TCTATGTGGC GGCCATTTCC ATGGCAGCCA TAGTCCATAC TCTGACCAAG 
GACTGTGCCA ATGGAGTAAT CGCCGGATGC GGATGCACGG AAAATGCTCT GAATGTACCC 
TGTGCCCACG AACCCACCAA GGCACTGGAG CAGTACGAGA AGCATTTCGG ATCGGGATCA 
GGAGCCATCG GTCACAATCG ACGGGTGGTG GGAGCTCTAC TACAAAGATC ACTGGAACAG 
GAATGCCGGT GCAAGCAACC AGGAGCTGTG CAGGGTGAAT GCCAGGAGGA GGAGTGTGTG 
GCGGTACTAA AGCCATTCGA AGCCATTGCC CAGGATCTCC TCCAAATGTA CGACGATGCC 
ATCCAACTAG AAGGAGCCAG TAGCAATCTA AAGATTATGT GGCAGAACAT TCCCCTAGAC 
TCGCTGGTCT TCATGCAGGA CTCGCCCAAC TACTGCGAAC GCGATGCCAC CGGATTGTGG 
AAAGGAACAC GGGGTCGCCA GTGCTCCAAG GATGGCAGTG GTTCTCTGGA GGAACGCCTC 
TCCTGCCAGC AGTTGTGCCG CGTGTGCGGA TACCGAGTGA GATCCCAGCA CGTGAGAACC 
GAGAGGAGGT GCAATTGCAA GCTGGTCTGG GGATTCCGAC TCCAGTGCGA TGTGTGCGTC 
CAGCTGGAAA GGCAGTACTC CTGCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650272.1
CDS	61..990
Translation	MIFAITFFMGITSTLAAVLEPMSYYQYTQFQAPLSWEDITGKGLKQALDSCQQSFQWQRWNCPSQDFVQKNSKPEENSPNREDVYVAAISMAAIVHTLTKDCANGVIAGCGCTENALNVPCAHEPTKALEQYEKHFGSGSGAIGHNRRVVGALLQRSLEQECRCKQPGAVQGECQEEECVAVLKPFEAIAQDLLQMYDDAIQLEGASSNLKIMWQNIPLDSLVFMQDSPNYCERDATGLWKGTRGRQCSKDGSGSLEERLSCQQLCRVCGYRVRSQHVRTERRCNCKLVWGFRLQCDVCVQLERQYSCY

Target ORF information:

RefSeq Version	NM_142015.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wnt inhibitor of dorsal (wntD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142015.2

ORF Insert Sequence:

ATGATTTTTG CCATCACATT CTTCATGGGT ATTACGAGCA CTCTGGCAGC AGTTTTGGAG 
CCCATGAGCT ACTACCAGTA CACCCAGTTC CAGGCACCAC TCTCCTGGGA GGATATAACC 
GGCAAGGGGC TGAAACAGGC CCTCGACAGC TGCCAACAGA GTTTCCAGTG GCAGCGATGG 
AACTGTCCGA GCCAGGATTT CGTGCAGAAG AATTCCAAGC CGGAGGAAAA CTCACCAAAT 
CGGGAGGATG TCTATGTGGC GGCCATTTCC ATGGCAGCCA TAGTCCATAC TCTGACCAAG 
GACTGTGCCA ATGGAGTAAT CGCCGGATGC GGATGCACGG AAAATGCTCT GAATGTACCC 
TGTGCCCACG AACCCACCAA GGCACTGGAG CAGTACGAGA AGCATTTCGG ATCGGGATCA 
GGAGCCATCG GTCACAATCG ACGGGTGGTG GGAGCTCTAC TACAAAGATC ACTGGAACAG 
GAATGCCGGT GCAAGCAACC AGGAGCTGTG CAGGGTGAAT GCCAGGAGGA GGAGTGTGTG 
GCGGTACTAA AGCCATTCGA AGCCATTGCC CAGGATCTCC TCCAAATGTA CGACGATGCC 
ATCCAACTAG AAGGAGCCAG TAGCAATCTA AAGATTATGT GGCAGAACAT TCCCCTAGAC 
TCGCTGGTCT TCATGCAGGA CTCGCCCAAC TACTGCGAAC GCGATGCCAC CGGATTGTGG 
AAAGGAACAC GGGGTCGCCA GTGCTCCAAG GATGGCAGTG GTTCTCTGGA GGAACGCCTC 
TCCTGCCAGC AGTTGTGCCG CGTGTGCGGA TACCGAGTGA GATCCCAGCA CGTGAGAACC 
GAGAGGAGGT GCAATTGCAA GCTGGTCTGG GGATTCCGAC TCCAGTGCGA TGTGTGCGTC 
CAGCTGGAAA GGCAGTACTC CTGCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wntD cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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