timeout cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	timeout
Entrez Gene ID	41615
Full Name	CG7855 gene product from transcript CG7855-RA
Synonyms	CG14381,CG14382,CG7855,CG8148,Dmel\CG7855,TIM2,dtim2,tim2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87D12-87D14

mRNA and Protein(s)

mRNA	Protein	Name
NM_079617.3	NP_524341.3	timeout

Pathways from BioSystems

Homologies

Homo sapiens (human)	TIMELESS	NP_003911.2
Canis lupus familiaris (dog)	TIMELESS	XP_538233.3
Pan troglodytes (chimpanzee)	TIMELESS	XP_509535.2
	LOC712835	XP_002808027.1
Danio rerio (zebrafish)	timeless	NP_001265529.1
Xenopus tropicalis (tropical clawed frog)	timeless	XP_002934575.1
Bos taurus (cattle)	TIMELESS	NP_001095541.2
Mus musculus (house mouse)	Timeless	NP_035719.1
Rattus norvegicus (Norway rat)	Timeless	NP_112630.1
Drosophila melanogaster (fruit fly)	timeout	NP_524341.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010787	XP_001689129.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila timeless2 is required for chromosome stability and circadian photoreception.
Title: Drosophila timeless2 is required for chromosome stability and circadian photoreception.

The following timeout gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the timeout cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04768	NM_079617.3 Latest version!	Drosophila melanogaster timeout (timeout), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

timeout ( NM_079617.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524341.3 Drosophila melanogaster timeout (timeout), mRNA.

Price & Availability↑

CloneID	OFa04768
Clone ID Related Accession (Same CDS sequence)	NM_079617.3
Accession Version	NM_079617.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	timeout
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Feb 27, 2003 this sequence version replaced NM_079617.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTATTC TGCTGGCGGA CATTGATGCC ACGTGTGCGG CCTTGGGATA CAGTGATGGA 
CAGAAATATC AAGCGGAGCC TGATGCCGCC GAGGGTCTCA AGCATCTGAT ATGGATTCTG 
CGTCGGGACC TGGACAACCA CGAGTATCGC CGTCATTTGG GCCGCTCCAA GGTGCTCCAA 
ACGGACTTGG TTTACATGCT GCCGGACTAT GTGCATCACG AGGAGCTATC CGATCTACTG 
ATCCGCCTGC TGGTCATCCT GACCAATCCG ACACTGCTGC TCTACCGGGA GGGTGCTCCC 
AAGGACAACC ATGGCCGGAA AGTGTTCATG GAGCTAATCG ACATCCTGCA GGGCTACAAA 
GCAGCCTTTG CCAAGGACAA AGTCTGGAGC GCCCTTTTCG AGAAGCTCAA GCAGGCTTTG 
GAAATAGCAT TTGCGATTCG CAGCGAAGAG CAGAATCTTC TGATTGAGCG CATCCTGGTC 
CTGGTGCGGA ACGTTCTGCA AGTGCCAGCG AATCCGGAGG CCGAGTGCCG GGCGGACAAT 
GATGCCAGTC TGCACGACCA GGTCATTTGG GCGTTGCATC AAACTGGAAT GCTGGACTTG 
GTGCTGTTTG TCATCTCCTC GCCGGATGAG GAGCAGTTCC ATTTGCACGG ACTGGAGATT 
GTTTGCCTGC TTTTCAGGGA ACAGAGTGCC GAATCCCTGG CGGACGCCTC CCTGCAGCGC 
AGTCTGAGCG AAAAGCAGCG GGACCAGCAG GAGCTCCTGG CCGCACGACG ACGGGAGCGT 
GCCCGCCGTC AGGCTCGTCC TCCACCAGGG CGGCATTCCC GTTTCGGCGG AACCTATGTC 
ATTCGGAACA TGAAGTCCGT TTCGGATCGG GATGTGATAT GCCACCAGGC TCTGGAGCGC 
GTGTCATCGA TCGATTTTGA CCGCGAGAAG CAGCAGCAGA AGCGCTCCCA CCGACACATC 
CAGGAGGAGG CGCAGGTGAC CCGTCGCAGT GCGTTCACCG TTAGATTGTG CCTGCGCGAA 
TACTGCATCG AGGTACTGCG CTCCGCTTAC AACACTCTGG TGAGGCAGGT GCGTCGGGTG 
CTGGAACGCA ACGCCGGATC GTCGAGTCAC GATGACTCCT ATCTATTGTG GGCCATACGA 
TTTTTCATGG AGTTCAATCG TTTGAGTGGT CTACAATTGC AGTTAGTCAG CGAATCGTTG 
AGCGTGCAAT GCTTCCACTG GGTCCTGACG CGCATGCAAC ATGACATGGA CATGATTGTG 
AGCGACAAGA AACAGGCGCG TCTCTGGGCC AAGCGACTGC ATGTAGCGCT TTTGACATTC 
CGGGAATTGC TCCAAAGCCT GCTGGCACTG CAGAAGCTAA AGGATGACAA CAACGCGCGA 
GCCCTTTTCG ATATGTTATT GAACAACGTG TGCTATGTCC TGGAGTATCG GGAGACGGTG 
CTGCATTTGT TAATGAACTA CAATGAGGCG CACAGCACAA AGGTTTTTCT GCGCGATGTG 
GTGGAAACGG CCAACGTTTT CATTAAAATG ATGGAGCGCT TCTGCCAGGA CTCTGTGGTG 
GTGCAAGACA AGAAGCGAGG TCGTGGCGCC TCCCGAAAGA AGAAGCAGGC GGCGACCAAG 
TCCAAGCCAC CAGCTGCACC ACAGCCCACT GAGGAAGAAT TGTCGAGCAA ATGGGCTGAA 
CTGGCAACGG AAGTGTGCAG CCTGCTGTCC ACGGAATTGG AAATGCCAGA GGATGAGCAG 
CCCCTGCCCT TCGATGCCGC ATCCGAGAAA TCCATTGATG ATCAGCGCGA GGATTGCATG 
ATTCGCATAA ATAAACTGCT TCGTTCCGAG AAACTGGACC AGGCCATCGC CCTTCTTCGT 
GCTGCCAGGG AAGTTTGGCC GGAGAACGAA GTGTTCGGTG CAATTTCCGC TGCACCGGAA 
GACGAGCTGC TGCTCCTGCG TGAGATTTTC ATGTCCAACA TTACAACAGT TGAGTCCGAG 
GATAAGGAAA ACGAAAAGGA CCAAAAGCAG GACGAGTACG AGGACGAAGA CGACGACTTG 
GATAACGAAG AATACGAAGA GCAGGATAAT GGACTCACCG AGAAAACGTT CAAATTTGAT 
GATTTCGCTC GCAGATTGCT GAATCCGAAA ATCGTTCGGG CTTGCACATT AGTGCTATCG 
GATTGGGCGG ATATACCCAC TCGATCTTTG AAAGCGGCCG TAACTATTCT ACATCGCATT 
GCCTACGGCT GCAAATGTGC GGGAATGCTT TTTCAGGCCA AGCTGTTTCG CATCTTTCAA 
CAAGTCTTCA GCGTGGAACG TGACGTCCAT CAGGAGGAGC TGCGCCGCCT GGCCATCTTT 
GTGGTGCGCA AATTTGTCGA GGTAGCCCCG ACTAATCCCA AAATCTATGC AGAGTTATTA 
TTTTACAAAG GCATCAGGGA GGCAAATGAG CTGGAGTCGG GCTATTGTGA CGCCTACGAA 
GCTGGCACCA AAGGAGCCTG GACCGAGGAG CAGGAGTCGG AGCTACGCTT CCTCTTCGAG 
GAGAACCAGC GCAATCCTGA AACAGATAAG GATGTGATTG ATTGGATTCT GGACAATTTG 
GTCGACAAGA CGCGCAACCG CCGCACCGTC CTGAAGAAGC TGAAGGAACT GGGCCTGCTA 
TTCAAGGCGC CCACCAAGCG TTCGACCAAG TCCGCCCAGA GCGGCAAAAA CGTCTGGCAG 
CCGGAAGAGG ATGACGAGCT AAGGAGCTTA TATGATCAGC ATCGCATTGA GCCGGACTGC 
CTAGAGCGGC TTGTCAATGA GTTCGCTGAA CGACGTAGCA AACAGCAGAT CATCAAGCGA 
ATGCTTCAGA TTCATTTGAT AGCGGATAAA TCCGAGATTC TGCCCGCCAA AAAGGGGCGT 
GGCAAAGACA AGCCCAAGAA AGACGTAGAA ATGGAAGGCG AGGGCGATGA GTTTGATTTT 
GCCGAGGAGC CCATGTTTGA GGACCAGGGG TATAAGAAGC CGAAGTCCAA ACCGAAAAAA 
GTCCAGAAGC GTCAGATGGT ACGCACACCC TTGGATGTAG GCACCATTAG AGCTCTAATC 
GGTCAGGTGG ATTCCGAAAA GTATCAATCG GCCATCGAGT GGCTGCAGGA ATGCCTGCAA 
GACGCTAGCG AAGATACCGA GGAAGCAGTC GAGGATGACG ATGGTGTGCC TCTCCTGCCT 
CTAATGGAGA ATCAAAAGAA TGCCATGGAA GATGGTGATT TCCAAAAGGT CCTGGTCGCC 
CTGGGCATGC AGCCACCAAT TTCGGGAATG GAAGCCTACT GGCGTATTCC TATATATCTG 
AACTCAGCCG ATTTGATATT ACGTTCCAAA ATCCTGGCAG GAGAAGAGGT TGATGCTGAG 
CCGGAAGACG AGGCTGCAGA CGATGAGGAT GGCGAGGAAG CCGAGGAGAG TGACGAAGAG 
GAGGACTTCC TTGAAAAGCA CAGCAGGCAG CGGAAGGAGA ACATAGCCAC CCAACAGCAG 
CGAAAACTTG ATAGTCTTAT GTTCAACCAG AGCGATGACG AAACCGAGCA GCGTGCTCCC 
CCAAAAGCCA AGGACAAACC CAAGGCCAGC GAAAAGAAGG CCAAGCGAAA GGTCAAAGGC 
AAAGCCAAGG AGTCAAAGGA CTGCAATGAC GATGGGACAG ATAAAGCAGA GCTCGAGAAA 
CCCAACACGG ATGATTTATT TAACCAATTG AGAGCCAAGC GAGCCACTAA AATCAAACTG 
AGCGATATGG CTCTAAATGA GTCAACAAAT GCCGCTCAAG AGGCGGACGA TGATGCATTT 
AACTTCAACT CGGAGGACTA TCGTGCCCGC CTGCTGGAAC TGGAGGATGA GGAGCAGAAC 
AGCAAGGATG CGGATGAGAA CGGCGGTGAC AAAGAGAACA TTACCAATAA GTCCCTGCAG 
CGTGCACGTC GCGTTAATGT AATTGACTCG GATAGCGACA ACGACGATGA TGGCGCCTTG 
CCCGCCGACA AGACAAACAA ACGCCCACGC AGCGACGACG ATGGGGATTC TGGCCACATG 
GATGCCAATG CCGAGGAGGA CAGCGATGAG GAGGCCAACT TTTTGGCTCG CAAAAAGCCA 
GCCAAAGTCG ATGGCGAGCC GGCCAAGCGG CGTCGGTTGG CCATCATCGA TGACGATGAT 
GAGGATGATT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524341.3
CDS	111..4265
Translation	MSILLADIDATCAALGYSDGQKYQAEPDAAEGLKHLIWILRRDLDNHEYRRHLGRSKVLQTDLVYMLPDYVHHEELSDLLIRLLVILTNPTLLLYREGAPKDNHGRKVFMELIDILQGYKAAFAKDKVWSALFEKLKQALEIAFAIRSEEQNLLIERILVLVRNVLQVPANPEAECRADNDASLHDQVIWALHQTGMLDLVLFVISSPDEEQFHLHGLEIVCLLFREQSAESLADASLQRSLSEKQRDQQELLAARRRERARRQARPPPGRHSRFGGTYVIRNMKSVSDRDVICHQALERVSSIDFDREKQQQKRSHRHIQEEAQVTRRSAFTVRLCLREYCIEVLRSAYNTLVRQVRRVLERNAGSSSHDDSYLLWAIRFFMEFNRLSGLQLQLVSESLSVQCFHWVLTRMQHDMDMIVSDKKQARLWAKRLHVALLTFRELLQSLLALQKLKDDNNARALFDMLLNNVCYVLEYRETVLHLLMNYNEAHSTKVFLRDVVETANVFIKMMERFCQDSVVVQDKKRGRGASRKKKQAATKSKPPAAPQPTEEELSSKWAELATEVCSLLSTELEMPEDEQPLPFDAASEKSIDDQREDCMIRINKLLRSEKLDQAIALLRAAREVWPENEVFGAISAAPEDELLLLREIFMSNITTVESEDKENEKDQKQDEYEDEDDDLDNEEYEEQDNGLTEKTFKFDDFARRLLNPKIVRACTLVLSDWADIPTRSLKAAVTILHRIAYGCKCAGMLFQAKLFRIFQQVFSVERDVHQEELRRLAIFVVRKFVEVAPTNPKIYAELLFYKGIREANELESGYCDAYEAGTKGAWTEEQESELRFLFEENQRNPETDKDVIDWILDNLVDKTRNRRTVLKKLKELGLLFKAPTKRSTKSAQSGKNVWQPEEDDELRSLYDQHRIEPDCLERLVNEFAERRSKQQIIKRMLQIHLIADKSEILPAKKGRGKDKPKKDVEMEGEGDEFDFAEEPMFEDQGYKKPKSKPKKVQKRQMVRTPLDVGTIRALIGQVDSEKYQSAIEWLQECLQDASEDTEEAVEDDDGVPLLPLMENQKNAMEDGDFQKVLVALGMQPPISGMEAYWRIPIYLNSADLILRSKILAGEEVDAEPEDEAADDEDGEEAEESDEEEDFLEKHSRQRKENIATQQQRKLDSLMFNQSDDETEQRAPPKAKDKPKASEKKAKRKVKGKAKESKDCNDDGTDKAELEKPNTDDLFNQLRAKRATKIKLSDMALNESTNAAQEADDDAFNFNSEDYRARLLELEDEEQNSKDADENGGDKENITNKSLQRARRVNVIDSDSDNDDDGALPADKTNKRPRSDDDGDSGHMDANAEEDSDEEANFLARKKPAKVDGEPAKRRRLAIIDDDDEDDF

Target ORF information:

RefSeq Version	NM_079617.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster timeout (timeout), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079617.3

ORF Insert Sequence:

ATGTCTATTC TGCTGGCGGA CATTGATGCC ACGTGTGCGG CCTTGGGATA CAGTGATGGA 
CAGAAATATC AAGCGGAGCC TGATGCCGCC GAGGGTCTCA AGCATCTGAT ATGGATTCTG 
CGTCGGGACC TGGACAACCA CGAGTATCGC CGTCATTTGG GCCGCTCCAA GGTGCTCCAA 
ACGGACTTGG TTTACATGCT GCCGGACTAT GTGCATCACG AGGAGCTATC CGATCTACTG 
ATCCGCCTGC TGGTCATCCT GACCAATCCG ACACTGCTGC TCTACCGGGA GGGTGCTCCC 
AAGGACAACC ATGGCCGGAA AGTGTTCATG GAGCTAATCG ACATCCTGCA GGGCTACAAA 
GCAGCCTTTG CCAAGGACAA AGTCTGGAGC GCCCTTTTCG AGAAGCTCAA GCAGGCTTTG 
GAAATAGCAT TTGCGATTCG CAGCGAAGAG CAGAATCTTC TGATTGAGCG CATCCTGGTC 
CTGGTGCGGA ACGTTCTGCA AGTGCCAGCG AATCCGGAGG CCGAGTGCCG GGCGGACAAT 
GATGCCAGTC TGCACGACCA GGTCATTTGG GCGTTGCATC AAACTGGAAT GCTGGACTTG 
GTGCTGTTTG TCATCTCCTC GCCGGATGAG GAGCAGTTCC ATTTGCACGG ACTGGAGATT 
GTTTGCCTGC TTTTCAGGGA ACAGAGTGCC GAATCCCTGG CGGACGCCTC CCTGCAGCGC 
AGTCTGAGCG AAAAGCAGCG GGACCAGCAG GAGCTCCTGG CCGCACGACG ACGGGAGCGT 
GCCCGCCGTC AGGCTCGTCC TCCACCAGGG CGGCATTCCC GTTTCGGCGG AACCTATGTC 
ATTCGGAACA TGAAGTCCGT TTCGGATCGG GATGTGATAT GCCACCAGGC TCTGGAGCGC 
GTGTCATCGA TCGATTTTGA CCGCGAGAAG CAGCAGCAGA AGCGCTCCCA CCGACACATC 
CAGGAGGAGG CGCAGGTGAC CCGTCGCAGT GCGTTCACCG TTAGATTGTG CCTGCGCGAA 
TACTGCATCG AGGTACTGCG CTCCGCTTAC AACACTCTGG TGAGGCAGGT GCGTCGGGTG 
CTGGAACGCA ACGCCGGATC GTCGAGTCAC GATGACTCCT ATCTATTGTG GGCCATACGA 
TTTTTCATGG AGTTCAATCG TTTGAGTGGT CTACAATTGC AGTTAGTCAG CGAATCGTTG 
AGCGTGCAAT GCTTCCACTG GGTCCTGACG CGCATGCAAC ATGACATGGA CATGATTGTG 
AGCGACAAGA AACAGGCGCG TCTCTGGGCC AAGCGACTGC ATGTAGCGCT TTTGACATTC 
CGGGAATTGC TCCAAAGCCT GCTGGCACTG CAGAAGCTAA AGGATGACAA CAACGCGCGA 
GCCCTTTTCG ATATGTTATT GAACAACGTG TGCTATGTCC TGGAGTATCG GGAGACGGTG 
CTGCATTTGT TAATGAACTA CAATGAGGCG CACAGCACAA AGGTTTTTCT GCGCGATGTG 
GTGGAAACGG CCAACGTTTT CATTAAAATG ATGGAGCGCT TCTGCCAGGA CTCTGTGGTG 
GTGCAAGACA AGAAGCGAGG TCGTGGCGCC TCCCGAAAGA AGAAGCAGGC GGCGACCAAG 
TCCAAGCCAC CAGCTGCACC ACAGCCCACT GAGGAAGAAT TGTCGAGCAA ATGGGCTGAA 
CTGGCAACGG AAGTGTGCAG CCTGCTGTCC ACGGAATTGG AAATGCCAGA GGATGAGCAG 
CCCCTGCCCT TCGATGCCGC ATCCGAGAAA TCCATTGATG ATCAGCGCGA GGATTGCATG 
ATTCGCATAA ATAAACTGCT TCGTTCCGAG AAACTGGACC AGGCCATCGC CCTTCTTCGT 
GCTGCCAGGG AAGTTTGGCC GGAGAACGAA GTGTTCGGTG CAATTTCCGC TGCACCGGAA 
GACGAGCTGC TGCTCCTGCG TGAGATTTTC ATGTCCAACA TTACAACAGT TGAGTCCGAG 
GATAAGGAAA ACGAAAAGGA CCAAAAGCAG GACGAGTACG AGGACGAAGA CGACGACTTG 
GATAACGAAG AATACGAAGA GCAGGATAAT GGACTCACCG AGAAAACGTT CAAATTTGAT 
GATTTCGCTC GCAGATTGCT GAATCCGAAA ATCGTTCGGG CTTGCACATT AGTGCTATCG 
GATTGGGCGG ATATACCCAC TCGATCTTTG AAAGCGGCCG TAACTATTCT ACATCGCATT 
GCCTACGGCT GCAAATGTGC GGGAATGCTT TTTCAGGCCA AGCTGTTTCG CATCTTTCAA 
CAAGTCTTCA GCGTGGAACG TGACGTCCAT CAGGAGGAGC TGCGCCGCCT GGCCATCTTT 
GTGGTGCGCA AATTTGTCGA GGTAGCCCCG ACTAATCCCA AAATCTATGC AGAGTTATTA 
TTTTACAAAG GCATCAGGGA GGCAAATGAG CTGGAGTCGG GCTATTGTGA CGCCTACGAA 
GCTGGCACCA AAGGAGCCTG GACCGAGGAG CAGGAGTCGG AGCTACGCTT CCTCTTCGAG 
GAGAACCAGC GCAATCCTGA AACAGATAAG GATGTGATTG ATTGGATTCT GGACAATTTG 
GTCGACAAGA CGCGCAACCG CCGCACCGTC CTGAAGAAGC TGAAGGAACT GGGCCTGCTA 
TTCAAGGCGC CCACCAAGCG TTCGACCAAG TCCGCCCAGA GCGGCAAAAA CGTCTGGCAG 
CCGGAAGAGG ATGACGAGCT AAGGAGCTTA TATGATCAGC ATCGCATTGA GCCGGACTGC 
CTAGAGCGGC TTGTCAATGA GTTCGCTGAA CGACGTAGCA AACAGCAGAT CATCAAGCGA 
ATGCTTCAGA TTCATTTGAT AGCGGATAAA TCCGAGATTC TGCCCGCCAA AAAGGGGCGT 
GGCAAAGACA AGCCCAAGAA AGACGTAGAA ATGGAAGGCG AGGGCGATGA GTTTGATTTT 
GCCGAGGAGC CCATGTTTGA GGACCAGGGG TATAAGAAGC CGAAGTCCAA ACCGAAAAAA 
GTCCAGAAGC GTCAGATGGT ACGCACACCC TTGGATGTAG GCACCATTAG AGCTCTAATC 
GGTCAGGTGG ATTCCGAAAA GTATCAATCG GCCATCGAGT GGCTGCAGGA ATGCCTGCAA 
GACGCTAGCG AAGATACCGA GGAAGCAGTC GAGGATGACG ATGGTGTGCC TCTCCTGCCT 
CTAATGGAGA ATCAAAAGAA TGCCATGGAA GATGGTGATT TCCAAAAGGT CCTGGTCGCC 
CTGGGCATGC AGCCACCAAT TTCGGGAATG GAAGCCTACT GGCGTATTCC TATATATCTG 
AACTCAGCCG ATTTGATATT ACGTTCCAAA ATCCTGGCAG GAGAAGAGGT TGATGCTGAG 
CCGGAAGACG AGGCTGCAGA CGATGAGGAT GGCGAGGAAG CCGAGGAGAG TGACGAAGAG 
GAGGACTTCC TTGAAAAGCA CAGCAGGCAG CGGAAGGAGA ACATAGCCAC CCAACAGCAG 
CGAAAACTTG ATAGTCTTAT GTTCAACCAG AGCGATGACG AAACCGAGCA GCGTGCTCCC 
CCAAAAGCCA AGGACAAACC CAAGGCCAGC GAAAAGAAGG CCAAGCGAAA GGTCAAAGGC 
AAAGCCAAGG AGTCAAAGGA CTGCAATGAC GATGGGACAG ATAAAGCAGA GCTCGAGAAA 
CCCAACACGG ATGATTTATT TAACCAATTG AGAGCCAAGC GAGCCACTAA AATCAAACTG 
AGCGATATGG CTCTAAATGA GTCAACAAAT GCCGCTCAAG AGGCGGACGA TGATGCATTT 
AACTTCAACT CGGAGGACTA TCGTGCCCGC CTGCTGGAAC TGGAGGATGA GGAGCAGAAC 
AGCAAGGATG CGGATGAGAA CGGCGGTGAC AAAGAGAACA TTACCAATAA GTCCCTGCAG 
CGTGCACGTC GCGTTAATGT AATTGACTCG GATAGCGACA ACGACGATGA TGGCGCCTTG 
CCCGCCGACA AGACAAACAA ACGCCCACGC AGCGACGACG ATGGGGATTC TGGCCACATG 
GATGCCAATG CCGAGGAGGA CAGCGATGAG GAGGCCAACT TTTTGGCTCG CAAAAAGCCA 
GCCAAAGTCG ATGGCGAGCC GGCCAAGCGG CGTCGGTTGG CCATCATCGA TGACGATGAT 
GAGGATGATT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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timeout cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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