yellow-f cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	yellow-f
Entrez Gene ID	41595
Full Name	CG18550 gene product from transcript CG18550-RC
Synonyms	CG18550,Dmel\CG18550,yell-f
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87D7-87D7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275587.1	NP_001262516.1	yellow-f, isoform B
NM_079611.2	NP_524335.1	yellow-f, isoform A
NM_001275588.1	NP_001262517.1	yellow-f, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	yellow-f	NP_524335.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004324	XP_313598.5
Drosophila melanogaster (fruit fly)	yellow-f2	NP_650247.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003096	XP_001237493.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003097	XP_312785.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identification of Drosophila melanogaster yellow-f and yellow-f2 proteins as dopachrome-conversion enzymes.
Title: Identification of Drosophila melanogaster yellow-f and yellow-f2 proteins as dopachrome-conversion enzymes.

The following yellow-f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the yellow-f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04746	NM_079611.2 Latest version!	Drosophila melanogaster yellow-f, transcript variant A (yellow-f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07635	NM_001275588.1 Latest version!	Drosophila melanogaster yellow-f, transcript variant C (yellow-f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07636	NM_001275587.1 Latest version!	Drosophila melanogaster yellow-f, transcript variant B (yellow-f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

yellow-f ( NM_079611.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524335.1 Drosophila melanogaster yellow-f, transcript variant A (yellow-f), mRNA.

Price & Availability↑

CloneID	OFa04746
Clone ID Related Accession (Same CDS sequence)	NM_079611.2
Accession Version	NM_079611.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	yellow-f, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Nov 6, 2002 this sequence version replaced NM_079611.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTATCGC TGGACGTTCT GCTCCTGTGC GCAATCTCAG GGTTCCAATT GCTAATCTCT 
GCAGATGGAG ATCCCATGAT TGAGGTCTTC AAGTGGAAGC AGTTGGACTT TTACAATCGC 
GGCGATGGCT ATAAGGATTT GTGGAACAGG ATCTGCATTC CAGATTCGCA TTTCTACAAC 
TCCCGCAAGT GCTTAGGCTC CTCATCCTCA GGATCCTTCA TCCAATACAA CAACGTGCCC 
CAAGGAGTAA CCCACTTTCG AGGACGCCTC TTTGTCACCG TGCCACGGCG ACAGCCGGGC 
ATTCCGTCCA CCTTAAACTA CATCGATCTG GCCAAGGATG GGTGGAGTCA GAGTCCCCAT 
CTACGGGCCT ATCCCAACTT GGCGGTGAAT CAGTATAATG CGAGCGAGCA GAACCTAGTG 
TCTGTCTATA GAACCTCAGT GGATGTCTGT GGGCGTCTCT GGTTCGTCGA CACCGGAATG 
CTGGAGTTTC CCAATAATCG CCAGCAGATT CGACATCCTA GCATTTGGGT GATCGATTTG 
GCTAACGATC GCTTATTGAA ACGATTTGAG ATTCCGCAGA GCATAGTGGA GATCGGACGT 
GGACTAGCCA GCATCACAAT CGATGTGGGC GCTCGGAGGT GCAACGATGC CTATGCCTAC 
ATTCCGGATT TGGTCAATCG CAGACTGCAC GTCTATCACC TGCGAAGCGA TAGAATTTGG 
TCCTTCGAGC ACAGCTTCTT TAACTTTGAT CCCCTTTCTG ATAATCTCAA CATTGGTGGG 
CAGACCTTCC GCTGGGACGA CGGGATTTTC TCGGCGACCT TGGGATCATA TAAGCCCGAT 
GGCAGCAGGG ATGTCTTCTT CCATCCTATG GCCAGCACGA ATGAGTTTGT GGTTAGCAAC 
AGGGTCTTGC AGCAGGAATT CAATGCAGCA CGTTCTGATC ATGGAGACGA CTTCCATCTT 
CTGGGAACTC GTGGTCCGTC CACCCAGTCC ACAATGCATA AATACGATCC GCGGACCGGG 
GTGATTTTCT TTGCCGAGGT CCAGAAGAGT GGAGTGGGCT GCTGGAAAAC CAGCAAGCCG 
TTCTCAACGG AAAACCATGG CTCCGTTTAT TCCAATTCAT CGGAGATGAT TTATCCCAGC 
GATTTGACCA TCGACGAGGA GGGATACATT TGGGTGATGT CCAACTCCAT GCCCATCTTT 
GTGTACTCCA AGCTGGATGT GGAGAAGTAC AATTTCCGAA TCTGGAGGCA GTCGACATTG 
CTGGCCAAGA GGGGAACAGT TTGTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524335.1
CDS	57..1346
Translation	MLSLDVLLLCAISGFQLLISADGDPMIEVFKWKQLDFYNRGDGYKDLWNRICIPDSHFYNSRKCLGSSSSGSFIQYNNVPQGVTHFRGRLFVTVPRRQPGIPSTLNYIDLAKDGWSQSPHLRAYPNLAVNQYNASEQNLVSVYRTSVDVCGRLWFVDTGMLEFPNNRQQIRHPSIWVIDLANDRLLKRFEIPQSIVEIGRGLASITIDVGARRCNDAYAYIPDLVNRRLHVYHLRSDRIWSFEHSFFNFDPLSDNLNIGGQTFRWDDGIFSATLGSYKPDGSRDVFFHPMASTNEFVVSNRVLQQEFNAARSDHGDDFHLLGTRGPSTQSTMHKYDPRTGVIFFAEVQKSGVGCWKTSKPFSTENHGSVYSNSSEMIYPSDLTIDEEGYIWVMSNSMPIFVYSKLDVEKYNFRIWRQSTLLAKRGTVCE

Target ORF information:

RefSeq Version	NM_079611.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster yellow-f, transcript variant A (yellow-f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079611.2

ORF Insert Sequence:

ATGTTATCGC TGGACGTTCT GCTCCTGTGC GCAATCTCAG GGTTCCAATT GCTAATCTCT 
GCAGATGGAG ATCCCATGAT TGAGGTCTTC AAGTGGAAGC AGTTGGACTT TTACAATCGC 
GGCGATGGCT ATAAGGATTT GTGGAACAGG ATCTGCATTC CAGATTCGCA TTTCTACAAC 
TCCCGCAAGT GCTTAGGCTC CTCATCCTCA GGATCCTTCA TCCAATACAA CAACGTGCCC 
CAAGGAGTAA CCCACTTTCG AGGACGCCTC TTTGTCACCG TGCCACGGCG ACAGCCGGGC 
ATTCCGTCCA CCTTAAACTA CATCGATCTG GCCAAGGATG GGTGGAGTCA GAGTCCCCAT 
CTACGGGCCT ATCCCAACTT GGCGGTGAAT CAGTATAATG CGAGCGAGCA GAACCTAGTG 
TCTGTCTATA GAACCTCAGT GGATGTCTGT GGGCGTCTCT GGTTCGTCGA CACCGGAATG 
CTGGAGTTTC CCAATAATCG CCAGCAGATT CGACATCCTA GCATTTGGGT GATCGATTTG 
GCTAACGATC GCTTATTGAA ACGATTTGAG ATTCCGCAGA GCATAGTGGA GATCGGACGT 
GGACTAGCCA GCATCACAAT CGATGTGGGC GCTCGGAGGT GCAACGATGC CTATGCCTAC 
ATTCCGGATT TGGTCAATCG CAGACTGCAC GTCTATCACC TGCGAAGCGA TAGAATTTGG 
TCCTTCGAGC ACAGCTTCTT TAACTTTGAT CCCCTTTCTG ATAATCTCAA CATTGGTGGG 
CAGACCTTCC GCTGGGACGA CGGGATTTTC TCGGCGACCT TGGGATCATA TAAGCCCGAT 
GGCAGCAGGG ATGTCTTCTT CCATCCTATG GCCAGCACGA ATGAGTTTGT GGTTAGCAAC 
AGGGTCTTGC AGCAGGAATT CAATGCAGCA CGTTCTGATC ATGGAGACGA CTTCCATCTT 
CTGGGAACTC GTGGTCCGTC CACCCAGTCC ACAATGCATA AATACGATCC GCGGACCGGG 
GTGATTTTCT TTGCCGAGGT CCAGAAGAGT GGAGTGGGCT GCTGGAAAAC CAGCAAGCCG 
TTCTCAACGG AAAACCATGG CTCCGTTTAT TCCAATTCAT CGGAGATGAT TTATCCCAGC 
GATTTGACCA TCGACGAGGA GGGATACATT TGGGTGATGT CCAACTCCAT GCCCATCTTT 
GTGTACTCCA AGCTGGATGT GGAGAAGTAC AATTTCCGAA TCTGGAGGCA GTCGACATTG 
CTGGCCAAGA GGGGAACAGT TTGTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

yellow-f ( NM_001275588.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262517.1 Drosophila melanogaster yellow-f, transcript variant C (yellow-f), mRNA.

Price & Availability↑

CloneID	OFa07635
Clone ID Related Accession (Same CDS sequence)	NM_001275588.1
Accession Version	NM_001275588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	yellow-f, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTATCGC TGGACGTTCT GCTCCTGTGC GCAATCTCAG GGTTCCAATT GCTAATCTCT 
GCAGATGGAG ATCCCATGAT TGAGGTCTTC AAGTGGAAGC AGTTGGACTT TTACAATCGC 
GGCGATGGCT ATAAGGATTT GTGGAACAGG ATCTGCATTC CAGGATCCTT CATCCAATAC 
AACAACGTGC CCCAAGGAGT AACCCACTTT CGAGGACGCC TCTTTGTCAC CGTGCCACGG 
CGACAGCCGG GCATTCCGTC CACCTTAAAC TACATCGATC TGGCCAAGGA TGGGTGGAGT 
CAGAGTCCCC ATCTACGGGC CTATCCCAAC TTGGCGGTGA ATCAGTATAA TGCGAGCGAG 
CAGAACCTAG TGTCTGTCTA TAGAACCTCA GTGGATGTCT GTGGGCGTCT CTGGTTCGTC 
GACACCGGAA TGCTGGAGTT TCCCAATAAT CGCCAGCAGA TTCGACATCC TAGCATTTGG 
GTGATCGATT TGGCTAACGA TCGCTTATTG AAACGATTTG AGATTCCGCA GAGCATAGTG 
GAGATCGGAC GTGGACTAGC CAGCATCACA ATCGATGTGG GCGCTCGGAG GTGCAACGAT 
GCCTATGCCT ACATTCCGGA TTTGGTCAAT CGCAGACTGC ACGTCTATCA CCTGCGAAGC 
GATAGAATTT GGTCCTTCGA GCACAGCTTC TTTAACTTTG ATCCCCTTTC TGATAATCTC 
AACATTGGTG GGCAGACCTT CCGCTGGGAC GACGGGATTT TCTCGGCGAC CTTGGGATCA 
TATAAGCCCG ATGGCAGCAG GGATGTCTTC TTCCATCCTA TGGCCAGCAC GAATGAGTTT 
GTGGTTAGCA ACAGGGTCTT GCAGCAGGAA TTCAATGCAG CACGTTCTGA TCATGGAGAC 
GACTTCCATC TTCTGGGAAC TCGTGGTCCG TCCACCCAGT CCACAATGCA TAAATACGAT 
CCGCGGACCG GGGTGATTTT CTTTGCCGAG GTCCAGAAGA GTGGAGTGGG CTGCTGGAAA 
ACCAGCAAGC CGTTCTCAAC GGAAAACCAT GGCTCCGTTT ATTCCAATTC ATCGGAGATG 
ATTTATCCCA GCGATTTGAC CATCGACGAG GAGGGATACA TTTGGGTGAT GTCCAACTCC 
ATGCCCATCT TTGTGTACTC CAAGCTGGAT GTGGAGAAGT ACAATTTCCG AATCTGGAGG 
CAGTCGACAT TGCTGGCCAA GAGGGGAACA GTTTGTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262517.1
CDS	57..1298
Translation	MLSLDVLLLCAISGFQLLISADGDPMIEVFKWKQLDFYNRGDGYKDLWNRICIPGSFIQYNNVPQGVTHFRGRLFVTVPRRQPGIPSTLNYIDLAKDGWSQSPHLRAYPNLAVNQYNASEQNLVSVYRTSVDVCGRLWFVDTGMLEFPNNRQQIRHPSIWVIDLANDRLLKRFEIPQSIVEIGRGLASITIDVGARRCNDAYAYIPDLVNRRLHVYHLRSDRIWSFEHSFFNFDPLSDNLNIGGQTFRWDDGIFSATLGSYKPDGSRDVFFHPMASTNEFVVSNRVLQQEFNAARSDHGDDFHLLGTRGPSTQSTMHKYDPRTGVIFFAEVQKSGVGCWKTSKPFSTENHGSVYSNSSEMIYPSDLTIDEEGYIWVMSNSMPIFVYSKLDVEKYNFRIWRQSTLLAKRGTVCE

Target ORF information:

RefSeq Version	NM_001275588.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster yellow-f, transcript variant C (yellow-f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275588.1

ORF Insert Sequence:

ATGTTATCGC TGGACGTTCT GCTCCTGTGC GCAATCTCAG GGTTCCAATT GCTAATCTCT 
GCAGATGGAG ATCCCATGAT TGAGGTCTTC AAGTGGAAGC AGTTGGACTT TTACAATCGC 
GGCGATGGCT ATAAGGATTT GTGGAACAGG ATCTGCATTC CAGGATCCTT CATCCAATAC 
AACAACGTGC CCCAAGGAGT AACCCACTTT CGAGGACGCC TCTTTGTCAC CGTGCCACGG 
CGACAGCCGG GCATTCCGTC CACCTTAAAC TACATCGATC TGGCCAAGGA TGGGTGGAGT 
CAGAGTCCCC ATCTACGGGC CTATCCCAAC TTGGCGGTGA ATCAGTATAA TGCGAGCGAG 
CAGAACCTAG TGTCTGTCTA TAGAACCTCA GTGGATGTCT GTGGGCGTCT CTGGTTCGTC 
GACACCGGAA TGCTGGAGTT TCCCAATAAT CGCCAGCAGA TTCGACATCC TAGCATTTGG 
GTGATCGATT TGGCTAACGA TCGCTTATTG AAACGATTTG AGATTCCGCA GAGCATAGTG 
GAGATCGGAC GTGGACTAGC CAGCATCACA ATCGATGTGG GCGCTCGGAG GTGCAACGAT 
GCCTATGCCT ACATTCCGGA TTTGGTCAAT CGCAGACTGC ACGTCTATCA CCTGCGAAGC 
GATAGAATTT GGTCCTTCGA GCACAGCTTC TTTAACTTTG ATCCCCTTTC TGATAATCTC 
AACATTGGTG GGCAGACCTT CCGCTGGGAC GACGGGATTT TCTCGGCGAC CTTGGGATCA 
TATAAGCCCG ATGGCAGCAG GGATGTCTTC TTCCATCCTA TGGCCAGCAC GAATGAGTTT 
GTGGTTAGCA ACAGGGTCTT GCAGCAGGAA TTCAATGCAG CACGTTCTGA TCATGGAGAC 
GACTTCCATC TTCTGGGAAC TCGTGGTCCG TCCACCCAGT CCACAATGCA TAAATACGAT 
CCGCGGACCG GGGTGATTTT CTTTGCCGAG GTCCAGAAGA GTGGAGTGGG CTGCTGGAAA 
ACCAGCAAGC CGTTCTCAAC GGAAAACCAT GGCTCCGTTT ATTCCAATTC ATCGGAGATG 
ATTTATCCCA GCGATTTGAC CATCGACGAG GAGGGATACA TTTGGGTGAT GTCCAACTCC 
ATGCCCATCT TTGTGTACTC CAAGCTGGAT GTGGAGAAGT ACAATTTCCG AATCTGGAGG 
CAGTCGACAT TGCTGGCCAA GAGGGGAACA GTTTGTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

yellow-f ( NM_001275587.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262516.1 Drosophila melanogaster yellow-f, transcript variant B (yellow-f), mRNA.

Price & Availability↑

CloneID	OFa07636
Clone ID Related Accession (Same CDS sequence)	NM_001275587.1
Accession Version	NM_001275587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	yellow-f, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTATCGC TGGACGTTCT GCTCCTGTGC GCAATCTCAG GGTTCCAATT GCTAATCTCT 
GCAGATGGAG ATCCCATGAT TGAGGTCTTC AAGTGGAAGC AGTTGGACTT TTACAATCGC 
GGCGATGGCT ATAAGGATTT GTGGAACAGG ATCTGCATTC CAGGCTCCTC ATCCTCAGGA 
TCCTTCATCC AATACAACAA CGTGCCCCAA GGAGTAACCC ACTTTCGAGG ACGCCTCTTT 
GTCACCGTGC CACGGCGACA GCCGGGCATT CCGTCCACCT TAAACTACAT CGATCTGGCC 
AAGGATGGGT GGAGTCAGAG TCCCCATCTA CGGGCCTATC CCAACTTGGC GGTGAATCAG 
TATAATGCGA GCGAGCAGAA CCTAGTGTCT GTCTATAGAA CCTCAGTGGA TGTCTGTGGG 
CGTCTCTGGT TCGTCGACAC CGGAATGCTG GAGTTTCCCA ATAATCGCCA GCAGATTCGA 
CATCCTAGCA TTTGGGTGAT CGATTTGGCT AACGATCGCT TATTGAAACG ATTTGAGATT 
CCGCAGAGCA TAGTGGAGAT CGGACGTGGA CTAGCCAGCA TCACAATCGA TGTGGGCGCT 
CGGAGGTGCA ACGATGCCTA TGCCTACATT CCGGATTTGG TCAATCGCAG ACTGCACGTC 
TATCACCTGC GAAGCGATAG AATTTGGTCC TTCGAGCACA GCTTCTTTAA CTTTGATCCC 
CTTTCTGATA ATCTCAACAT TGGTGGGCAG ACCTTCCGCT GGGACGACGG GATTTTCTCG 
GCGACCTTGG GATCATATAA GCCCGATGGC AGCAGGGATG TCTTCTTCCA TCCTATGGCC 
AGCACGAATG AGTTTGTGGT TAGCAACAGG GTCTTGCAGC AGGAATTCAA TGCAGCACGT 
TCTGATCATG GAGACGACTT CCATCTTCTG GGAACTCGTG GTCCGTCCAC CCAGTCCACA 
ATGCATAAAT ACGATCCGCG GACCGGGGTG ATTTTCTTTG CCGAGGTCCA GAAGAGTGGA 
GTGGGCTGCT GGAAAACCAG CAAGCCGTTC TCAACGGAAA ACCATGGCTC CGTTTATTCC 
AATTCATCGG AGATGATTTA TCCCAGCGAT TTGACCATCG ACGAGGAGGG ATACATTTGG 
GTGATGTCCA ACTCCATGCC CATCTTTGTG TACTCCAAGC TGGATGTGGA GAAGTACAAT 
TTCCGAATCT GGAGGCAGTC GACATTGCTG GCCAAGAGGG GAACAGTTTG TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262516.1
CDS	57..1313
Translation	MLSLDVLLLCAISGFQLLISADGDPMIEVFKWKQLDFYNRGDGYKDLWNRICIPGSSSSGSFIQYNNVPQGVTHFRGRLFVTVPRRQPGIPSTLNYIDLAKDGWSQSPHLRAYPNLAVNQYNASEQNLVSVYRTSVDVCGRLWFVDTGMLEFPNNRQQIRHPSIWVIDLANDRLLKRFEIPQSIVEIGRGLASITIDVGARRCNDAYAYIPDLVNRRLHVYHLRSDRIWSFEHSFFNFDPLSDNLNIGGQTFRWDDGIFSATLGSYKPDGSRDVFFHPMASTNEFVVSNRVLQQEFNAARSDHGDDFHLLGTRGPSTQSTMHKYDPRTGVIFFAEVQKSGVGCWKTSKPFSTENHGSVYSNSSEMIYPSDLTIDEEGYIWVMSNSMPIFVYSKLDVEKYNFRIWRQSTLLAKRGTVCE

Target ORF information:

RefSeq Version	NM_001275587.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster yellow-f, transcript variant B (yellow-f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275587.1

ORF Insert Sequence:

ATGTTATCGC TGGACGTTCT GCTCCTGTGC GCAATCTCAG GGTTCCAATT GCTAATCTCT 
GCAGATGGAG ATCCCATGAT TGAGGTCTTC AAGTGGAAGC AGTTGGACTT TTACAATCGC 
GGCGATGGCT ATAAGGATTT GTGGAACAGG ATCTGCATTC CAGGCTCCTC ATCCTCAGGA 
TCCTTCATCC AATACAACAA CGTGCCCCAA GGAGTAACCC ACTTTCGAGG ACGCCTCTTT 
GTCACCGTGC CACGGCGACA GCCGGGCATT CCGTCCACCT TAAACTACAT CGATCTGGCC 
AAGGATGGGT GGAGTCAGAG TCCCCATCTA CGGGCCTATC CCAACTTGGC GGTGAATCAG 
TATAATGCGA GCGAGCAGAA CCTAGTGTCT GTCTATAGAA CCTCAGTGGA TGTCTGTGGG 
CGTCTCTGGT TCGTCGACAC CGGAATGCTG GAGTTTCCCA ATAATCGCCA GCAGATTCGA 
CATCCTAGCA TTTGGGTGAT CGATTTGGCT AACGATCGCT TATTGAAACG ATTTGAGATT 
CCGCAGAGCA TAGTGGAGAT CGGACGTGGA CTAGCCAGCA TCACAATCGA TGTGGGCGCT 
CGGAGGTGCA ACGATGCCTA TGCCTACATT CCGGATTTGG TCAATCGCAG ACTGCACGTC 
TATCACCTGC GAAGCGATAG AATTTGGTCC TTCGAGCACA GCTTCTTTAA CTTTGATCCC 
CTTTCTGATA ATCTCAACAT TGGTGGGCAG ACCTTCCGCT GGGACGACGG GATTTTCTCG 
GCGACCTTGG GATCATATAA GCCCGATGGC AGCAGGGATG TCTTCTTCCA TCCTATGGCC 
AGCACGAATG AGTTTGTGGT TAGCAACAGG GTCTTGCAGC AGGAATTCAA TGCAGCACGT 
TCTGATCATG GAGACGACTT CCATCTTCTG GGAACTCGTG GTCCGTCCAC CCAGTCCACA 
ATGCATAAAT ACGATCCGCG GACCGGGGTG ATTTTCTTTG CCGAGGTCCA GAAGAGTGGA 
GTGGGCTGCT GGAAAACCAG CAAGCCGTTC TCAACGGAAA ACCATGGCTC CGTTTATTCC 
AATTCATCGG AGATGATTTA TCCCAGCGAT TTGACCATCG ACGAGGAGGG ATACATTTGG 
GTGATGTCCA ACTCCATGCC CATCTTTGTG TACTCCAAGC TGGATGTGGA GAAGTACAAT 
TTCCGAATCT GGAGGCAGTC GACATTGCTG GCCAAGAGGG GAACAGTTTG TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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yellow-f cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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