mus308 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mus308
Entrez Gene ID	41571
Full Name	mutagen-sensitive 308
Synonyms	0443/09,AAF54858,CG6019,Dmel\CG6019,Mus308,Nuc3,POLQ,Pol theta,PolQ,mus-308
General protein information	Preferred Names mutagen-sensitive 308 Names CG6019 gene product from transcript CG6019-RA CG6019-PA DNA polymerase theta Nuclease-3 mus308-PA pol theta
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87C6-87C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_079609.3	NP_524333.1	mutagen-sensitive 308

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	POLQ	XP_545125.3
Gallus gallus (chicken)	POLQ	XP_416549.3
Xenopus tropicalis (tropical clawed frog)	polq	NP_001184156.1
Bos taurus (cattle)	POLQ	XP_002684835.1
Rattus norvegicus (Norway rat)	Polq	NP_001099348.1
Danio rerio (zebrafish)	si:rp71-1p14.5	XP_694437.6
Neurospora crassa	NCU07411	XP_958186.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007533	XP_564726.3
Homo sapiens (human)	POLQ	NP_955452.3
	POLQ	XP_001111254.2
Drosophila melanogaster (fruit fly)	mus308	NP_524333.1
Magnaporthe oryzae (rice blast fungus)	MGG_15295	XP_003711003.1
Pan troglodytes (chimpanzee)	POLQ	XP_001165150.2
Mus musculus (house mouse)	Polq	NP_084253.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both polymerase and ATPase activities are required during microhomology-mediated end joining and interstrand crosslink repair.
Title: Drosophila DNA polymerase theta utilizes both helicase-like and polymerase domains during microhomology-mediated end joining and interstrand crosslink repair.

DNA polymerase theta, encoded by the mus308 gene, plays a crucial role in DNA ligase 4-independent alternative end joining.
Title: Dual roles for DNA polymerase theta in alternative end-joining repair of double-strand breaks in Drosophila.

mus 308 gene product localizes throughout nuclei in wild-type cells but appears to be absent in a mus 308 mutant Drosophila
Title: The Drosophila mus 308 gene product, implicated in tolerance of DNA interstrand crosslinks, is a nuclear protein found in both ovaries and embryos.

The following mus308 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mus308 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04723	NM_079609.3 Latest version!	Drosophila melanogaster mutagen-sensitive 308 (mus308), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mus308 ( NM_079609.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524333.1 Drosophila melanogaster mutagen-sensitive 308 (mus308), mRNA.

Price & Availability↑

CloneID	OFa04723
Clone ID Related Accession (Same CDS sequence)	NM_079609.3
Accession Version	NM_079609.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mutagen-sensitive 308
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_079609.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTCT CGCAGTCGTT CAACTTCGGA AACAGCACCT TAATGGCGCT GGAGAAAGGA 
ATGCAAGCGG ACGACAAGGA GAATGCACAG CCAGGAAACG GAAATATCCA AGTCCAAAGT 
GCCGGCAATG AGGTAAATAG CGAGATCCAG GAAATTAACT CGGAGTTTTT CCGGGACGAG 
TTCTCCTACG AAGTGAATCA GGCCCATAAG CCAGCTGAAC AATCAGTGGT CAATGTGTCT 
CAGGTTCAGC AGCATATGGC AGTTGTTTCC AATCAGGACT CTGAGGATCA GAGCCGCTCA 
TCCGCTTTGA ATGACCAAAT CTGCACACAG TCTTCGTTCG AGGGCGAGGA CGCAGGCGCG 
GATGCAGTGC TGGATCAGCC CAATCTGGAT GAGAACAGCT TTTTGTGTCC TGCGCAGGAT 
GAAGAGGCTT CCGAGCAGCT GAAGGAAGAC ATCTTGCACA GTCATTCCGT ATTGGCCAAG 
CAGGAATTCT ATCAAGAGAT TTCGCAAGTC ACGCAGAACC TGAGCAGCAT GTCGCCCAAC 
CAACTGCGAG TGTCGCCCAA CTCCTCGAGG ATCCGCGAAG CGATGCCCGA AAGACCAGCA 
ATGCCACTTG ATCTCAACAC GCTTAGATCA ATAAGCGCAT GGAACCTGCC CATGAGCATT 
CAAGCGGAGT ACAAGAAGAA GGGTGTTGTC GATATGTTCG ACTGGCAGGT GGAGTGTCTC 
TCCAAGCCTA GATTGCTGTT TGAGCACTGC AACCTGGTTT ATTCAGCACC CACGTCGGCA 
GGCAAGACGT TGGTTAGTGA GATACTGATG CTGAAAACCG TACTGGAGCG AGGTAAAAAG 
GTGCTGCTCA TACTGCCCTT CATCTCGGTG GTGCGCGAGA AAATGTTCTA CATGCAGGAT 
CTGCTCACGC CGGCTGGTTA CCGCGTGGAG GGATTCTACG GCGGCTATAC GCCACCAGGA 
GGCTTTGAGA GCCTTCATGT GGCCATTTGC ACAATTGAGA AGGCGAACTC CATTGTGAAC 
AAGCTGATGG AGCAGGGCAA ACTGGAAACC ATTGGCATGG TGGTGGTAGA CGAAGTGCAT 
CTCATTTCGG ACAAGGGACG CGGCTATATC CTGGAACTTC TGCTAGCCAA GATCCTCTAC 
ATGTCCCGTC GCAATGGACT GCAGATCCAG GTGATCACCA TGTCGGCCAC ACTCGAGAAT 
GTGCAGCTGC TGCAGAGCTG GCTTGACGCC GAGTTGTACA TCACAAACTA CCGACCCGTT 
GCTCTAAAGG AAATGATTAA GGTGGGCACA GTAATTTATG ACCACCGTTT GAAATTAGTT 
CGAGATGTGG CCAAGCAAAA GGTACTTTTG AAAGGCCTGG AAAACGATTC CGATGATGTG 
GCCCTTCTCT GCATAGAGAC GTTGCTGGAG GGATGCTCGG TAATCGTGTT CTGCCCCTCG 
AAAGATTGGT GCGAGAACCT GGCCGTTCAA TTGGCCACCG CAATCCATGT CCAAATCAAA 
TCTGAAACAG TGCTGGGCCA GCGATTGAGA ACAAATCTAA ATCCGAGGGC CATTGCAGAA 
GTGAAGCAAC AACTCAGGGA CATTCCCACA GGTCTCGATG GGGTCATGTC GAAGGCGATC 
ACCTACGCCT GCGCGTTTCA CCACGCCGGC CTAACTACGG AGGAGCGGGA CATTATTGAA 
GCCTCGTTTA AGGCAGGAGC ACTGAAGGTT CTAGTCGCCA CCAGCACACT GAGTTCGGGA 
GTGAATCTGC CCGCTCGGCG CGTCCTGATA CGCTCGCCAT TATTTGGGGG CAAGCAAATG 
AGTTCCCTAA CGTACCGCCA GATGATCGGA CGAGCTGGAC GCATGGGAAA GGATACGCTG 
GGTGAGTCTA TTCTCATCTG CAACGAGATC AATGCCCGAA TGGGAAGAGA CCTGGTCGTA 
TCGGAGCTGC AGCCCATTAC TTCCTGCCTC GATATGGACG GAAGCACCCA CTTGAAGCGC 
GCTCTACTCG AGGTAATTTC TTCTGGTGTA GCGAACACCA AAGAAGACAT CGACTTCTTC 
GTGAATTGTA CTCTGCTGAG TGCCCAAAAG GCCTTCCACG CAAAGGAGAA ACCACCCGAT 
GAGGAATCGG ACGCCAACTA CATTAACGAT GCCCTCGACT TTCTTGTGGA GTACGAGTTC 
GTTCGCCTGC AAAGGAACGA GGAAAGGGAG ACAGCGGTCT ATGTGGCCAC TCGTCTCGGT 
GCCGCCTGTC TGGCTTCCTC TATGCCGCCC ACCGACGGCC TCATTCTTTT TGCGGAACTC 
CAGAAATCTC GTCGCTCCTT CGTCCTAGAG TCAGAACTGC ATGCTGTTTA CCTAGTAACG 
CCCTATTCTG TCTGCTACCA ACTTCAAGAT ATCGACTGGC TGCTGTATGT GCATATGTGG 
GAAAAGTTAA GTTCGCCCAT GAAAAAAGTG GGCGAGCTTG TTGGAGTCAG GGACGCCTTT 
CTCTACAAAG CGCTGCGTGG TCAAACAAAA CTAGACTACA AGCAAATGCA AATACATAAG 
CGTTTCTACA TAGCTCTGGC TCTGGAGGAA CTGGTAAACG AAACACCCAT AAACGTCGTG 
GTCCACAAGT ATAAGTGCCA CCGAGGAATG CTACAGAGTC TGCAGCAAAT GGCCTCGACC 
TTTGCAGGCA TTGTTACTGC TTTCTGCAAT TCTCTGCAGT GGTCCACACT CGCCCTGATC 
GTGTCCCAAT TTAAGGATCG TCTCTTCTTT GGCATTCACA GGGATCTTAT CGATCTGATG 
CGAATACCCG ATCTGTCGCA GAAACGAGCT CGTGCTCTCT TTGATGCTGG CATCACCAGT 
TTGGTAGAGC TAGCGGGCGC TGATCCCGTA GAATTGGAAA AGGTGCTCTA TAACTCAATC 
AGCTTCGACT CTGCAAAGCA ACACGACCAC GAGAACGCGG ATGAAGCGGC CAAGCGAAAT 
GTAGTTAGAA ATTTCTATAT CACAGGCAAG GCGGGGATGA CAGTGAGCGA GGCTGCCAAA 
CTACTGATTG GTGAAGCTCG ACAATTTGTT CAGCACGAGA TCGGACTGGG CACAATTAAA 
TGGACCCAAA CTCAGGCGGG AGTGGAAATA GCTTCGAGGG CAATACACGA TGGCGGGGAG 
GTAGACCTCC ACATGTCCCT GGAGGAGGAG CAGCCGCCAG TAAAGAGGAA ATTGTCCATC 
GAAGAAAATG GCACAGCCAA TTCACAAAAA AATCCCAGAC TCGAAACAGT AGTGGACACA 
CAGCGAGGCT ATAAAGTTGA TAAGAATATT GCTAATCAAA GCAAGATGAA CCCGAATCTT 
AAAGAAATCG ATGCCCAAAA TAAGGCGCGA AGGAATTCAA CTGCACACAT GGATAACCTA 
AACCCGATTA GCAATGACCC TTGTCAGAAC AACGTAAACG TAAAAACGGC ACAACCCATA 
ATATCCAATT TGAATGACAT CCAGAAACAA GGTTCTCAGA TTGAAAAGAT GAAAATCAAT 
CCAGCAACTG TGGTGTGTAG TCCACAATTA GCAAATGAAG AAAAACCGAG TACCAGTCAA 
AGTGCTAGAA GGAAGCTCGT TAATGAGGGA ATGGCCGAAC GCCGCAGGGT CGCTTTAATG 
AAGATTCAGC AACGCACCCA AAAAGAGAAC CAGTCTAAGG ATCAGCCAAT ACAGGCGTCG 
AGGTCTAACC AACTTTCAAG TCCGGTTAAT AGAACACCCG CAAATCGATG GACACAATCG 
GAAAACCCAA ACAATGAGAT GAACAATAGT CAACTGCCCC GCAGAAATCC ACGAAATCAA 
TCACCAGTAC CCAATGCAAA CCGCACTGCG TCAAGGAAGG TTTCCAACGC AGAGGAGGAT 
TTGTTTATGG CAGACGATTC CTTTATGCTG AATACCGGTC TAGCCGCTGC TCTCACGGCC 
GCTGAGAGCA AAATTGCCTC TTGCACGGAG GCGGACGTGA TACCTAGCTC GCAACCTAAG 
GAGCCGGAAG TGATCGGTGC ACTGACTCCA CATGCATCCA GACTGAAGCG TTCCGACCAG 
TTGCGTTCAC AGCGCATACA AAGTCCCAGC CCCACCCCGC AACGTGAAAT CGAAATTGAT 
TTGGAATCGA AAAACGAATC CAATGGGGTC TCCTCTATGG AAATCTCGGA CATGTCTATG 
GAGAACCCAC TCATGAAAAA TCCTTTACAT CTAAATGCCT CGCACATTAT GAGCTGTTCG 
AAAGTCGATG AAACTGCGTC GAGTTTCTCT TCTATAGATA TAATCGATGT GTGTGGCCAC 
CGGAATGCGT TTCAAGCTGC TATCATCGAG ATCAATAACG CCACGCGTTT GGGATTTAGC 
GTTGGCCTGC AGGCTCAAGC GGGCAAACAG AAGCCGCTAA TTGGATCCAA CCTCTTGATC 
AATCAAGTTG CGGCGGCCGA GAACCGGGAG GCGGCTGCCA GGGAACGTGT GCTATTCCAA 
GTGGACGACA CCAACTTCAT CTCTGGTGTG TCTTTCTGTC TTGCGGACAA TGTTGCCTAC 
TATTGGAATA TGCAAATTGA CGAACGTGCA GCATATCAGG GAGTGCCCAC CCCTCTCAAG 
GTGCAGGAAT TGTGCAACCT GATGGCTCGA AAGGATCTCA CGCTGGTCAT GCACGACGGA 
AAGGAGCAGC TTAAGATGCT GCGCAAAGCG ATTCCGCAGC TAAAAAGGAT TAGCGCCAAG 
CTGGAGGATG CCAAGGTCGC AAACTGGCTG CTGCAGCCGG ACAAAACTGT GAATTTTCTC 
AATATGTGCC AGACATTCGC CCCGGAATGC ACTGGCCTTG CGAATCTCTG TGGCAGTGGT 
CGTGGTTATA GCAGCTACGG GCTTGATACC TCCAGTGCCA TTTTGCCGAG GATTAGGACG 
GCCATCGAAT CCTGCGTAAC GCTGCACATA CTCCAGGGAC AGACTGAGAA CCTAAGCAGG 
ATTGGCAATG GTGACTTACT TAAGTTTTTC CACGATATCG AAATGCCCAT TCAATTGACT 
CTTTGTCAAA TGGAGCTTGT GGGCTTTCCC GCCCAGAAGC AGCGACTCCA ACAGCTTTAT 
CAGCGCATGG TGGCCGTTAT GAAGAAAGTG GAAACAAAGA TTTATGAGCA GCATGGTTCC 
CGTTTCAATC TGGGCTCGTC ACAAGCTGTT GCCAAGGTGC TGGGACTCCA CCGGAAAGCA 
AAAGGACGAG TTACCACCTC ACGCCAGGTG CTGGAGAAGT TGAACTCGCC CATTTCGCAT 
TTGATACTAG GCTATCGAAA GCTGTCTGGG CTCTTGGCCA AAAGCATTCA GCCGCTAATG 
GAGTGCTGCC AGGCAGACAG AATTCATGGC CAGAGCATTA CATATACGGC AACGGGTCGT 
ATTTCCATGA CGGAGCCGAA CCTGCAAAAT GTCGCCAAAG AATTCAGCAT ACAAGTGGGC 
TCTGATGTGG TCCACATATC CTGTAGGTCA CCATTCATGC CCACGGATGA GAGCCGATGT 
CTTTTGTCGG CTGACTTCTG TCAGCTGGAG ATGCGGATCC TTGCCCACAT GTCTCAGGAT 
AAGGCTCTGT TGGAGGTGAT GAAATCGTCA CAGGACCTGT TCATCGCAAT TGCAGCGCAT 
TGGAACAAAA TCGAAGAGTC CGAAGTGACG CAGGACCTCC GCAACAGCAC CAAGCAGGTG 
TGCTACGGAA TCGTCTACGG CATGGGCATG CGGTCCCTGG CCGAGTCGTT AAACTGCAGT 
GAGCAGGAGG CGCGGATGAT ATCCGATCAG TTTCACCAGG CGTACAAGGG TATTCGCGAT 
TACACCACGA GGGTGGTCAA TTTCGCACGC AGCAAGGGAT TTGTTGAGAC CATTACTGGA 
CGGCGACGGT ATTTGGAAAA TATCAACAGT GATGTGGAGC ATCTAAAAAA TCAGGCCGAG 
CGCCAGGCCG TTAACTCCAC CATCCAAGGA TCGGCTGCGG ATATTGCGAA GAACGCTATT 
CTGAAGATGG AAAAGAACAT TGAACGTTAT CGCGAAAAGC TGGCATTGGG TGACAATTCA 
GTTGACCTGG TAATGCACCT ACATGACGAA CTCATTTTCG AGGTGCCAAC GGGAAAGGCC 
AAAAAAATAG CCAAAGTCCT TAGCCTCACC ATGGAGAACT GCGTGAAACT CAGCGTGCCA 
CTCAAGGTGA AGCTGAGGAT CGGACGCAGT TGGGGTGAAT TTAAGGAAGT TAGCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524333.1
CDS	93..6272
Translation	MAFSQSFNFGNSTLMALEKGMQADDKENAQPGNGNIQVQSAGNEVNSEIQEINSEFFRDEFSYEVNQAHKPAEQSVVNVSQVQQHMAVVSNQDSEDQSRSSALNDQICTQSSFEGEDAGADAVLDQPNLDENSFLCPAQDEEASEQLKEDILHSHSVLAKQEFYQEISQVTQNLSSMSPNQLRVSPNSSRIREAMPERPAMPLDLNTLRSISAWNLPMSIQAEYKKKGVVDMFDWQVECLSKPRLLFEHCNLVYSAPTSAGKTLVSEILMLKTVLERGKKVLLILPFISVVREKMFYMQDLLTPAGYRVEGFYGGYTPPGGFESLHVAICTIEKANSIVNKLMEQGKLETIGMVVVDEVHLISDKGRGYILELLLAKILYMSRRNGLQIQVITMSATLENVQLLQSWLDAELYITNYRPVALKEMIKVGTVIYDHRLKLVRDVAKQKVLLKGLENDSDDVALLCIETLLEGCSVIVFCPSKDWCENLAVQLATAIHVQIKSETVLGQRLRTNLNPRAIAEVKQQLRDIPTGLDGVMSKAITYACAFHHAGLTTEERDIIEASFKAGALKVLVATSTLSSGVNLPARRVLIRSPLFGGKQMSSLTYRQMIGRAGRMGKDTLGESILICNEINARMGRDLVVSELQPITSCLDMDGSTHLKRALLEVISSGVANTKEDIDFFVNCTLLSAQKAFHAKEKPPDEESDANYINDALDFLVEYEFVRLQRNEERETAVYVATRLGAACLASSMPPTDGLILFAELQKSRRSFVLESELHAVYLVTPYSVCYQLQDIDWLLYVHMWEKLSSPMKKVGELVGVRDAFLYKALRGQTKLDYKQMQIHKRFYIALALEELVNETPINVVVHKYKCHRGMLQSLQQMASTFAGIVTAFCNSLQWSTLALIVSQFKDRLFFGIHRDLIDLMRIPDLSQKRARALFDAGITSLVELAGADPVELEKVLYNSISFDSAKQHDHENADEAAKRNVVRNFYITGKAGMTVSEAAKLLIGEARQFVQHEIGLGTIKWTQTQAGVEIASRAIHDGGEVDLHMSLEEEQPPVKRKLSIEENGTANSQKNPRLETVVDTQRGYKVDKNIANQSKMNPNLKEIDAQNKARRNSTAHMDNLNPISNDPCQNNVNVKTAQPIISNLNDIQKQGSQIEKMKINPATVVCSPQLANEEKPSTSQSARRKLVNEGMAERRRVALMKIQQRTQKENQSKDQPIQASRSNQLSSPVNRTPANRWTQSENPNNEMNNSQLPRRNPRNQSPVPNANRTASRKVSNAEEDLFMADDSFMLNTGLAAALTAAESKIASCTEADVIPSSQPKEPEVIGALTPHASRLKRSDQLRSQRIQSPSPTPQREIEIDLESKNESNGVSSMEISDMSMENPLMKNPLHLNASHIMSCSKVDETASSFSSIDIIDVCGHRNAFQAAIIEINNATRLGFSVGLQAQAGKQKPLIGSNLLINQVAAAENREAAARERVLFQVDDTNFISGVSFCLADNVAYYWNMQIDERAAYQGVPTPLKVQELCNLMARKDLTLVMHDGKEQLKMLRKAIPQLKRISAKLEDAKVANWLLQPDKTVNFLNMCQTFAPECTGLANLCGSGRGYSSYGLDTSSAILPRIRTAIESCVTLHILQGQTENLSRIGNGDLLKFFHDIEMPIQLTLCQMELVGFPAQKQRLQQLYQRMVAVMKKVETKIYEQHGSRFNLGSSQAVAKVLGLHRKAKGRVTTSRQVLEKLNSPISHLILGYRKLSGLLAKSIQPLMECCQADRIHGQSITYTATGRISMTEPNLQNVAKEFSIQVGSDVVHISCRSPFMPTDESRCLLSADFCQLEMRILAHMSQDKALLEVMKSSQDLFIAIAAHWNKIEESEVTQDLRNSTKQVCYGIVYGMGMRSLAESLNCSEQEARMISDQFHQAYKGIRDYTTRVVNFARSKGFVETITGRRRYLENINSDVEHLKNQAERQAVNSTIQGSAADIAKNAILKMEKNIERYREKLALGDNSVDLVMHLHDELIFEVPTGKAKKIAKVLSLTMENCVKLSVPLKVKLRIGRSWGEFKEVSV

Target ORF information:

RefSeq Version	NM_079609.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mutagen-sensitive 308 (mus308), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079609.3

ORF Insert Sequence:

ATGGCATTCT CGCAGTCGTT CAACTTCGGA AACAGCACCT TAATGGCGCT GGAGAAAGGA 
ATGCAAGCGG ACGACAAGGA GAATGCACAG CCAGGAAACG GAAATATCCA AGTCCAAAGT 
GCCGGCAATG AGGTAAATAG CGAGATCCAG GAAATTAACT CGGAGTTTTT CCGGGACGAG 
TTCTCCTACG AAGTGAATCA GGCCCATAAG CCAGCTGAAC AATCAGTGGT CAATGTGTCT 
CAGGTTCAGC AGCATATGGC AGTTGTTTCC AATCAGGACT CTGAGGATCA GAGCCGCTCA 
TCCGCTTTGA ATGACCAAAT CTGCACACAG TCTTCGTTCG AGGGCGAGGA CGCAGGCGCG 
GATGCAGTGC TGGATCAGCC CAATCTGGAT GAGAACAGCT TTTTGTGTCC TGCGCAGGAT 
GAAGAGGCTT CCGAGCAGCT GAAGGAAGAC ATCTTGCACA GTCATTCCGT ATTGGCCAAG 
CAGGAATTCT ATCAAGAGAT TTCGCAAGTC ACGCAGAACC TGAGCAGCAT GTCGCCCAAC 
CAACTGCGAG TGTCGCCCAA CTCCTCGAGG ATCCGCGAAG CGATGCCCGA AAGACCAGCA 
ATGCCACTTG ATCTCAACAC GCTTAGATCA ATAAGCGCAT GGAACCTGCC CATGAGCATT 
CAAGCGGAGT ACAAGAAGAA GGGTGTTGTC GATATGTTCG ACTGGCAGGT GGAGTGTCTC 
TCCAAGCCTA GATTGCTGTT TGAGCACTGC AACCTGGTTT ATTCAGCACC CACGTCGGCA 
GGCAAGACGT TGGTTAGTGA GATACTGATG CTGAAAACCG TACTGGAGCG AGGTAAAAAG 
GTGCTGCTCA TACTGCCCTT CATCTCGGTG GTGCGCGAGA AAATGTTCTA CATGCAGGAT 
CTGCTCACGC CGGCTGGTTA CCGCGTGGAG GGATTCTACG GCGGCTATAC GCCACCAGGA 
GGCTTTGAGA GCCTTCATGT GGCCATTTGC ACAATTGAGA AGGCGAACTC CATTGTGAAC 
AAGCTGATGG AGCAGGGCAA ACTGGAAACC ATTGGCATGG TGGTGGTAGA CGAAGTGCAT 
CTCATTTCGG ACAAGGGACG CGGCTATATC CTGGAACTTC TGCTAGCCAA GATCCTCTAC 
ATGTCCCGTC GCAATGGACT GCAGATCCAG GTGATCACCA TGTCGGCCAC ACTCGAGAAT 
GTGCAGCTGC TGCAGAGCTG GCTTGACGCC GAGTTGTACA TCACAAACTA CCGACCCGTT 
GCTCTAAAGG AAATGATTAA GGTGGGCACA GTAATTTATG ACCACCGTTT GAAATTAGTT 
CGAGATGTGG CCAAGCAAAA GGTACTTTTG AAAGGCCTGG AAAACGATTC CGATGATGTG 
GCCCTTCTCT GCATAGAGAC GTTGCTGGAG GGATGCTCGG TAATCGTGTT CTGCCCCTCG 
AAAGATTGGT GCGAGAACCT GGCCGTTCAA TTGGCCACCG CAATCCATGT CCAAATCAAA 
TCTGAAACAG TGCTGGGCCA GCGATTGAGA ACAAATCTAA ATCCGAGGGC CATTGCAGAA 
GTGAAGCAAC AACTCAGGGA CATTCCCACA GGTCTCGATG GGGTCATGTC GAAGGCGATC 
ACCTACGCCT GCGCGTTTCA CCACGCCGGC CTAACTACGG AGGAGCGGGA CATTATTGAA 
GCCTCGTTTA AGGCAGGAGC ACTGAAGGTT CTAGTCGCCA CCAGCACACT GAGTTCGGGA 
GTGAATCTGC CCGCTCGGCG CGTCCTGATA CGCTCGCCAT TATTTGGGGG CAAGCAAATG 
AGTTCCCTAA CGTACCGCCA GATGATCGGA CGAGCTGGAC GCATGGGAAA GGATACGCTG 
GGTGAGTCTA TTCTCATCTG CAACGAGATC AATGCCCGAA TGGGAAGAGA CCTGGTCGTA 
TCGGAGCTGC AGCCCATTAC TTCCTGCCTC GATATGGACG GAAGCACCCA CTTGAAGCGC 
GCTCTACTCG AGGTAATTTC TTCTGGTGTA GCGAACACCA AAGAAGACAT CGACTTCTTC 
GTGAATTGTA CTCTGCTGAG TGCCCAAAAG GCCTTCCACG CAAAGGAGAA ACCACCCGAT 
GAGGAATCGG ACGCCAACTA CATTAACGAT GCCCTCGACT TTCTTGTGGA GTACGAGTTC 
GTTCGCCTGC AAAGGAACGA GGAAAGGGAG ACAGCGGTCT ATGTGGCCAC TCGTCTCGGT 
GCCGCCTGTC TGGCTTCCTC TATGCCGCCC ACCGACGGCC TCATTCTTTT TGCGGAACTC 
CAGAAATCTC GTCGCTCCTT CGTCCTAGAG TCAGAACTGC ATGCTGTTTA CCTAGTAACG 
CCCTATTCTG TCTGCTACCA ACTTCAAGAT ATCGACTGGC TGCTGTATGT GCATATGTGG 
GAAAAGTTAA GTTCGCCCAT GAAAAAAGTG GGCGAGCTTG TTGGAGTCAG GGACGCCTTT 
CTCTACAAAG CGCTGCGTGG TCAAACAAAA CTAGACTACA AGCAAATGCA AATACATAAG 
CGTTTCTACA TAGCTCTGGC TCTGGAGGAA CTGGTAAACG AAACACCCAT AAACGTCGTG 
GTCCACAAGT ATAAGTGCCA CCGAGGAATG CTACAGAGTC TGCAGCAAAT GGCCTCGACC 
TTTGCAGGCA TTGTTACTGC TTTCTGCAAT TCTCTGCAGT GGTCCACACT CGCCCTGATC 
GTGTCCCAAT TTAAGGATCG TCTCTTCTTT GGCATTCACA GGGATCTTAT CGATCTGATG 
CGAATACCCG ATCTGTCGCA GAAACGAGCT CGTGCTCTCT TTGATGCTGG CATCACCAGT 
TTGGTAGAGC TAGCGGGCGC TGATCCCGTA GAATTGGAAA AGGTGCTCTA TAACTCAATC 
AGCTTCGACT CTGCAAAGCA ACACGACCAC GAGAACGCGG ATGAAGCGGC CAAGCGAAAT 
GTAGTTAGAA ATTTCTATAT CACAGGCAAG GCGGGGATGA CAGTGAGCGA GGCTGCCAAA 
CTACTGATTG GTGAAGCTCG ACAATTTGTT CAGCACGAGA TCGGACTGGG CACAATTAAA 
TGGACCCAAA CTCAGGCGGG AGTGGAAATA GCTTCGAGGG CAATACACGA TGGCGGGGAG 
GTAGACCTCC ACATGTCCCT GGAGGAGGAG CAGCCGCCAG TAAAGAGGAA ATTGTCCATC 
GAAGAAAATG GCACAGCCAA TTCACAAAAA AATCCCAGAC TCGAAACAGT AGTGGACACA 
CAGCGAGGCT ATAAAGTTGA TAAGAATATT GCTAATCAAA GCAAGATGAA CCCGAATCTT 
AAAGAAATCG ATGCCCAAAA TAAGGCGCGA AGGAATTCAA CTGCACACAT GGATAACCTA 
AACCCGATTA GCAATGACCC TTGTCAGAAC AACGTAAACG TAAAAACGGC ACAACCCATA 
ATATCCAATT TGAATGACAT CCAGAAACAA GGTTCTCAGA TTGAAAAGAT GAAAATCAAT 
CCAGCAACTG TGGTGTGTAG TCCACAATTA GCAAATGAAG AAAAACCGAG TACCAGTCAA 
AGTGCTAGAA GGAAGCTCGT TAATGAGGGA ATGGCCGAAC GCCGCAGGGT CGCTTTAATG 
AAGATTCAGC AACGCACCCA AAAAGAGAAC CAGTCTAAGG ATCAGCCAAT ACAGGCGTCG 
AGGTCTAACC AACTTTCAAG TCCGGTTAAT AGAACACCCG CAAATCGATG GACACAATCG 
GAAAACCCAA ACAATGAGAT GAACAATAGT CAACTGCCCC GCAGAAATCC ACGAAATCAA 
TCACCAGTAC CCAATGCAAA CCGCACTGCG TCAAGGAAGG TTTCCAACGC AGAGGAGGAT 
TTGTTTATGG CAGACGATTC CTTTATGCTG AATACCGGTC TAGCCGCTGC TCTCACGGCC 
GCTGAGAGCA AAATTGCCTC TTGCACGGAG GCGGACGTGA TACCTAGCTC GCAACCTAAG 
GAGCCGGAAG TGATCGGTGC ACTGACTCCA CATGCATCCA GACTGAAGCG TTCCGACCAG 
TTGCGTTCAC AGCGCATACA AAGTCCCAGC CCCACCCCGC AACGTGAAAT CGAAATTGAT 
TTGGAATCGA AAAACGAATC CAATGGGGTC TCCTCTATGG AAATCTCGGA CATGTCTATG 
GAGAACCCAC TCATGAAAAA TCCTTTACAT CTAAATGCCT CGCACATTAT GAGCTGTTCG 
AAAGTCGATG AAACTGCGTC GAGTTTCTCT TCTATAGATA TAATCGATGT GTGTGGCCAC 
CGGAATGCGT TTCAAGCTGC TATCATCGAG ATCAATAACG CCACGCGTTT GGGATTTAGC 
GTTGGCCTGC AGGCTCAAGC GGGCAAACAG AAGCCGCTAA TTGGATCCAA CCTCTTGATC 
AATCAAGTTG CGGCGGCCGA GAACCGGGAG GCGGCTGCCA GGGAACGTGT GCTATTCCAA 
GTGGACGACA CCAACTTCAT CTCTGGTGTG TCTTTCTGTC TTGCGGACAA TGTTGCCTAC 
TATTGGAATA TGCAAATTGA CGAACGTGCA GCATATCAGG GAGTGCCCAC CCCTCTCAAG 
GTGCAGGAAT TGTGCAACCT GATGGCTCGA AAGGATCTCA CGCTGGTCAT GCACGACGGA 
AAGGAGCAGC TTAAGATGCT GCGCAAAGCG ATTCCGCAGC TAAAAAGGAT TAGCGCCAAG 
CTGGAGGATG CCAAGGTCGC AAACTGGCTG CTGCAGCCGG ACAAAACTGT GAATTTTCTC 
AATATGTGCC AGACATTCGC CCCGGAATGC ACTGGCCTTG CGAATCTCTG TGGCAGTGGT 
CGTGGTTATA GCAGCTACGG GCTTGATACC TCCAGTGCCA TTTTGCCGAG GATTAGGACG 
GCCATCGAAT CCTGCGTAAC GCTGCACATA CTCCAGGGAC AGACTGAGAA CCTAAGCAGG 
ATTGGCAATG GTGACTTACT TAAGTTTTTC CACGATATCG AAATGCCCAT TCAATTGACT 
CTTTGTCAAA TGGAGCTTGT GGGCTTTCCC GCCCAGAAGC AGCGACTCCA ACAGCTTTAT 
CAGCGCATGG TGGCCGTTAT GAAGAAAGTG GAAACAAAGA TTTATGAGCA GCATGGTTCC 
CGTTTCAATC TGGGCTCGTC ACAAGCTGTT GCCAAGGTGC TGGGACTCCA CCGGAAAGCA 
AAAGGACGAG TTACCACCTC ACGCCAGGTG CTGGAGAAGT TGAACTCGCC CATTTCGCAT 
TTGATACTAG GCTATCGAAA GCTGTCTGGG CTCTTGGCCA AAAGCATTCA GCCGCTAATG 
GAGTGCTGCC AGGCAGACAG AATTCATGGC CAGAGCATTA CATATACGGC AACGGGTCGT 
ATTTCCATGA CGGAGCCGAA CCTGCAAAAT GTCGCCAAAG AATTCAGCAT ACAAGTGGGC 
TCTGATGTGG TCCACATATC CTGTAGGTCA CCATTCATGC CCACGGATGA GAGCCGATGT 
CTTTTGTCGG CTGACTTCTG TCAGCTGGAG ATGCGGATCC TTGCCCACAT GTCTCAGGAT 
AAGGCTCTGT TGGAGGTGAT GAAATCGTCA CAGGACCTGT TCATCGCAAT TGCAGCGCAT 
TGGAACAAAA TCGAAGAGTC CGAAGTGACG CAGGACCTCC GCAACAGCAC CAAGCAGGTG 
TGCTACGGAA TCGTCTACGG CATGGGCATG CGGTCCCTGG CCGAGTCGTT AAACTGCAGT 
GAGCAGGAGG CGCGGATGAT ATCCGATCAG TTTCACCAGG CGTACAAGGG TATTCGCGAT 
TACACCACGA GGGTGGTCAA TTTCGCACGC AGCAAGGGAT TTGTTGAGAC CATTACTGGA 
CGGCGACGGT ATTTGGAAAA TATCAACAGT GATGTGGAGC ATCTAAAAAA TCAGGCCGAG 
CGCCAGGCCG TTAACTCCAC CATCCAAGGA TCGGCTGCGG ATATTGCGAA GAACGCTATT 
CTGAAGATGG AAAAGAACAT TGAACGTTAT CGCGAAAAGC TGGCATTGGG TGACAATTCA 
GTTGACCTGG TAATGCACCT ACATGACGAA CTCATTTTCG AGGTGCCAAC GGGAAAGGCC 
AAAAAAATAG CCAAAGTCCT TAGCCTCACC ATGGAGAACT GCGTGAAACT CAGCGTGCCA 
CTCAAGGTGA AGCTGAGGAT CGGACGCAGT TGGGGTGAAT TTAAGGAAGT TAGCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mus308 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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