CG6225 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG6225
Entrez Gene ID	41559
Full Name	CG6225 gene product from transcript CG6225-RB
Synonyms	Dmel\CG6225
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87C3-87C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001104289.2	NP_001097759.1	uncharacterized protein, isoform B
NM_141964.3	NP_650221.1	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004109	XP_312988.5
Drosophila melanogaster (fruit fly)	CG6225	NP_001097759.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG6225 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG6225 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04709	NM_141964.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG6225), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa04709	NM_001104289.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG6225), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG6225 ( NM_001104289.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097759.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG6225), mRNA.

Price & Availability↑

CloneID	OFa04709
Clone ID Related Accession (Same CDS sequence)	NM_001104289.2 , NM_141964.3
Accession Version	NM_001104289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104289.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACAG CCAAATGGAT TTGGCGCTTG GCGCTGAGCA TCTTGCTGGT CGTGGGCTTT 
GTGGCTGCGG AAAAGCCGAC TGGCTATCAT CGCGAGATTT GCCAATACCG CAAGGGCAAA 
CATATCCAGC CCATGAGTGT TTATCACAAT CGCCTGATGG CGATGCGCGA ACAAATGCAG 
ATTAGAGCCA CGCTGCAGGG ACCGGAGATC TATGGCTACA TACTGCCCTC CACGGATGAG 
CATCTCAACC AGGAGGTGGC AGCGCGAGAT CAGAGATTGC GATATCTGTC CGGCTTCTCT 
GGAGTGCGTG CCTTTGCTGC CGTGACCAGT CACGGAGCAG CCATTTGGGT GGAGAACAGA 
TATGCCCAGC AGGCCGATGG AGAGCTGGAG TGCGACTGGG AGATCTATCT GACCAGCGGG 
AATGTATCAG TGGCCGACTG GTTGGGGAGC CATGTTCACA TCGACAAGCG AGTGGGTGCC 
GATCCCCATC TGGTGCCGCA CTCCCTTTGG GTTCAGTGGG AGCGAGAGCT GGAGGACAAG 
TTCCTTAAGC TGGTCAGGAT CAATACCAAT CTGGTGGATC ACATTTGGGG CGATGAGCGA 
CCGGAGATGC CCAAGAACCA GGTGATAAAA GTGCACGAGA AGCACTTCGC CGGCGAGAGT 
TGGCAGGACA AGGTCAAGGA GTTGAGAAGG CGACTGGCGC ATCTGGGCTG CGATGCCATG 
GTGGTCACTT CGCTCACCGA GATTGCCTAC TTGCTAAACA TTCGGGGCAC AGACATTCCG 
TATACTCCTG TGATTAAGTC ATACGCCGTG ATCAGTCGGG ATGATATATT CTTCTATGTG 
GACCACTCAA AGATTAGTCT GGGCATTGAC CTTCATCTCC GCACAGACTG CTTCAATGAA 
GACTGCGTGA AAATCAAAGA GTACAATCAG ATCTGGAGTG ACATTCGTAC CTATGCTCAG 
ATCTGGAAGC GCGTCCTTGT ACCCGCTCCT TGTGTACAGG ATCTTGGAGC GTCGGAGGCG 
ATATACACCT CCATGCCAGG CAAAATTGTC GTCTGGGAGA TCTCACCCAT TATCTTTATG 
CGGGCACAGA AAAACTCCGA TGAACAGGCC GGTATGAGAA GGGCACACAT CCGGGATGGC 
GCCGCCATTT GTGAGGCCAT GAGCAACATG GAGACTAGGT TCCATACCGA GCAGTGGACC 
GAGGAGAAGA TTAAGTACGA GGTAGAGCTC TGGCGTTTGT CCCAGAAGCA CGCCAAGGGC 
TTGTCCCTGC GAACGGTGGT GGCCTATGGA GAACACTCCG CCCTGCCCTA CTACATCTCC 
AGCAATGTGA CCAACATCGA GGTCTCCGAC CAGAGTCTGC TGGTCATCGA GTCTGGGGGT 
CAGTATCTGG AGGGCACCAC GGACGTGTCG CGCACCTTCA TTTTTGGTGA GCCCACGCAC 
GAGATGAAAA AGGCCTACAC GAATGTTCTG GCTGGAATTC TGCATCTGGC ACAACTCAAG 
TTTCCGTCCG ATCTGAAGCC CTCCGAGGTG GACGCCTTGG TGCGCAGCAT GGTTTGGAAG 
GACATGACGG ACTTCCCGCA GGCAACAGGT CATGGCATTG GATCCTTTGG ATCTGTGGAA 
GAACCTCCTA TATCAGTTTC CTATGGCAAA AATAGCAGTT TTCACTTCAA GCAGGGCTAC 
TTCTTCTCTA GCGAATCCGG CTACTACAAA CGCGATGACT TTGGCGTTCG TCTGAAGAAC 
GTGCTCGAGG TTGTGGACAC TGGCCATACA CATCCCAGTG GAGCACGCTT CCTTGCATTC 
CGGGACGTCA CCATGGTGCC GTATGAGCCC AAACTGATCG ACAGTACGCT CCTGAGTGCC 
GCGGAGAAGC GTCTCCTAAA TGAGTACAAT GCCAAGATCC GGAACGATAT TGGGGACGAA 
CTGAAGCGAC TGGGCAACAT GCGGGCCTTC TACTGGATGA TGAACCAAAC GCGTCACATT 
CGGGAGTATC TGCCTGAGGA TGAGTACCGC TCTGCGACCG GAGCAGGAAG CTGTGGTCAC 
TCCGCCAGTC CCCTGATACT ACTGGGACTG GGACTAATGG TTATCCTTTC TGCGATTATA 
CCCTCGCGAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097759.1
CDS	474..2588
Translation	MTTAKWIWRLALSILLVVGFVAAEKPTGYHREICQYRKGKHIQPMSVYHNRLMAMREQMQIRATLQGPEIYGYILPSTDEHLNQEVAARDQRLRYLSGFSGVRAFAAVTSHGAAIWVENRYAQQADGELECDWEIYLTSGNVSVADWLGSHVHIDKRVGADPHLVPHSLWVQWERELEDKFLKLVRINTNLVDHIWGDERPEMPKNQVIKVHEKHFAGESWQDKVKELRRRLAHLGCDAMVVTSLTEIAYLLNIRGTDIPYTPVIKSYAVISRDDIFFYVDHSKISLGIDLHLRTDCFNEDCVKIKEYNQIWSDIRTYAQIWKRVLVPAPCVQDLGASEAIYTSMPGKIVVWEISPIIFMRAQKNSDEQAGMRRAHIRDGAAICEAMSNMETRFHTEQWTEEKIKYEVELWRLSQKHAKGLSLRTVVAYGEHSALPYYISSNVTNIEVSDQSLLVIESGGQYLEGTTDVSRTFIFGEPTHEMKKAYTNVLAGILHLAQLKFPSDLKPSEVDALVRSMVWKDMTDFPQATGHGIGSFGSVEEPPISVSYGKNSSFHFKQGYFFSSESGYYKRDDFGVRLKNVLEVVDTGHTHPSGARFLAFRDVTMVPYEPKLIDSTLLSAAEKRLLNEYNAKIRNDIGDELKRLGNMRAFYWMMNQTRHIREYLPEDEYRSATGAGSCGHSASPLILLGLGLMVILSAIIPSRS

Target ORF information:

RefSeq Version	NM_001104289.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG6225), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104289.2

ORF Insert Sequence:

ATGACGACAG CCAAATGGAT TTGGCGCTTG GCGCTGAGCA TCTTGCTGGT CGTGGGCTTT 
GTGGCTGCGG AAAAGCCGAC TGGCTATCAT CGCGAGATTT GCCAATACCG CAAGGGCAAA 
CATATCCAGC CCATGAGTGT TTATCACAAT CGCCTGATGG CGATGCGCGA ACAAATGCAG 
ATTAGAGCCA CGCTGCAGGG ACCGGAGATC TATGGCTACA TACTGCCCTC CACGGATGAG 
CATCTCAACC AGGAGGTGGC AGCGCGAGAT CAGAGATTGC GATATCTGTC CGGCTTCTCT 
GGAGTGCGTG CCTTTGCTGC CGTGACCAGT CACGGAGCAG CCATTTGGGT GGAGAACAGA 
TATGCCCAGC AGGCCGATGG AGAGCTGGAG TGCGACTGGG AGATCTATCT GACCAGCGGG 
AATGTATCAG TGGCCGACTG GTTGGGGAGC CATGTTCACA TCGACAAGCG AGTGGGTGCC 
GATCCCCATC TGGTGCCGCA CTCCCTTTGG GTTCAGTGGG AGCGAGAGCT GGAGGACAAG 
TTCCTTAAGC TGGTCAGGAT CAATACCAAT CTGGTGGATC ACATTTGGGG CGATGAGCGA 
CCGGAGATGC CCAAGAACCA GGTGATAAAA GTGCACGAGA AGCACTTCGC CGGCGAGAGT 
TGGCAGGACA AGGTCAAGGA GTTGAGAAGG CGACTGGCGC ATCTGGGCTG CGATGCCATG 
GTGGTCACTT CGCTCACCGA GATTGCCTAC TTGCTAAACA TTCGGGGCAC AGACATTCCG 
TATACTCCTG TGATTAAGTC ATACGCCGTG ATCAGTCGGG ATGATATATT CTTCTATGTG 
GACCACTCAA AGATTAGTCT GGGCATTGAC CTTCATCTCC GCACAGACTG CTTCAATGAA 
GACTGCGTGA AAATCAAAGA GTACAATCAG ATCTGGAGTG ACATTCGTAC CTATGCTCAG 
ATCTGGAAGC GCGTCCTTGT ACCCGCTCCT TGTGTACAGG ATCTTGGAGC GTCGGAGGCG 
ATATACACCT CCATGCCAGG CAAAATTGTC GTCTGGGAGA TCTCACCCAT TATCTTTATG 
CGGGCACAGA AAAACTCCGA TGAACAGGCC GGTATGAGAA GGGCACACAT CCGGGATGGC 
GCCGCCATTT GTGAGGCCAT GAGCAACATG GAGACTAGGT TCCATACCGA GCAGTGGACC 
GAGGAGAAGA TTAAGTACGA GGTAGAGCTC TGGCGTTTGT CCCAGAAGCA CGCCAAGGGC 
TTGTCCCTGC GAACGGTGGT GGCCTATGGA GAACACTCCG CCCTGCCCTA CTACATCTCC 
AGCAATGTGA CCAACATCGA GGTCTCCGAC CAGAGTCTGC TGGTCATCGA GTCTGGGGGT 
CAGTATCTGG AGGGCACCAC GGACGTGTCG CGCACCTTCA TTTTTGGTGA GCCCACGCAC 
GAGATGAAAA AGGCCTACAC GAATGTTCTG GCTGGAATTC TGCATCTGGC ACAACTCAAG 
TTTCCGTCCG ATCTGAAGCC CTCCGAGGTG GACGCCTTGG TGCGCAGCAT GGTTTGGAAG 
GACATGACGG ACTTCCCGCA GGCAACAGGT CATGGCATTG GATCCTTTGG ATCTGTGGAA 
GAACCTCCTA TATCAGTTTC CTATGGCAAA AATAGCAGTT TTCACTTCAA GCAGGGCTAC 
TTCTTCTCTA GCGAATCCGG CTACTACAAA CGCGATGACT TTGGCGTTCG TCTGAAGAAC 
GTGCTCGAGG TTGTGGACAC TGGCCATACA CATCCCAGTG GAGCACGCTT CCTTGCATTC 
CGGGACGTCA CCATGGTGCC GTATGAGCCC AAACTGATCG ACAGTACGCT CCTGAGTGCC 
GCGGAGAAGC GTCTCCTAAA TGAGTACAAT GCCAAGATCC GGAACGATAT TGGGGACGAA 
CTGAAGCGAC TGGGCAACAT GCGGGCCTTC TACTGGATGA TGAACCAAAC GCGTCACATT 
CGGGAGTATC TGCCTGAGGA TGAGTACCGC TCTGCGACCG GAGCAGGAAG CTGTGGTCAC 
TCCGCCAGTC CCCTGATACT ACTGGGACTG GGACTAATGG TTATCCTTTC TGCGATTATA 
CCCTCGCGAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG6225 ( NM_141964.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650221.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG6225), mRNA.

Price & Availability↑

CloneID	OFa04709
Clone ID Related Accession (Same CDS sequence)	NM_001104289.2 , NM_141964.3
Accession Version	NM_141964.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141964.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACAG CCAAATGGAT TTGGCGCTTG GCGCTGAGCA TCTTGCTGGT CGTGGGCTTT 
GTGGCTGCGG AAAAGCCGAC TGGCTATCAT CGCGAGATTT GCCAATACCG CAAGGGCAAA 
CATATCCAGC CCATGAGTGT TTATCACAAT CGCCTGATGG CGATGCGCGA ACAAATGCAG 
ATTAGAGCCA CGCTGCAGGG ACCGGAGATC TATGGCTACA TACTGCCCTC CACGGATGAG 
CATCTCAACC AGGAGGTGGC AGCGCGAGAT CAGAGATTGC GATATCTGTC CGGCTTCTCT 
GGAGTGCGTG CCTTTGCTGC CGTGACCAGT CACGGAGCAG CCATTTGGGT GGAGAACAGA 
TATGCCCAGC AGGCCGATGG AGAGCTGGAG TGCGACTGGG AGATCTATCT GACCAGCGGG 
AATGTATCAG TGGCCGACTG GTTGGGGAGC CATGTTCACA TCGACAAGCG AGTGGGTGCC 
GATCCCCATC TGGTGCCGCA CTCCCTTTGG GTTCAGTGGG AGCGAGAGCT GGAGGACAAG 
TTCCTTAAGC TGGTCAGGAT CAATACCAAT CTGGTGGATC ACATTTGGGG CGATGAGCGA 
CCGGAGATGC CCAAGAACCA GGTGATAAAA GTGCACGAGA AGCACTTCGC CGGCGAGAGT 
TGGCAGGACA AGGTCAAGGA GTTGAGAAGG CGACTGGCGC ATCTGGGCTG CGATGCCATG 
GTGGTCACTT CGCTCACCGA GATTGCCTAC TTGCTAAACA TTCGGGGCAC AGACATTCCG 
TATACTCCTG TGATTAAGTC ATACGCCGTG ATCAGTCGGG ATGATATATT CTTCTATGTG 
GACCACTCAA AGATTAGTCT GGGCATTGAC CTTCATCTCC GCACAGACTG CTTCAATGAA 
GACTGCGTGA AAATCAAAGA GTACAATCAG ATCTGGAGTG ACATTCGTAC CTATGCTCAG 
ATCTGGAAGC GCGTCCTTGT ACCCGCTCCT TGTGTACAGG ATCTTGGAGC GTCGGAGGCG 
ATATACACCT CCATGCCAGG CAAAATTGTC GTCTGGGAGA TCTCACCCAT TATCTTTATG 
CGGGCACAGA AAAACTCCGA TGAACAGGCC GGTATGAGAA GGGCACACAT CCGGGATGGC 
GCCGCCATTT GTGAGGCCAT GAGCAACATG GAGACTAGGT TCCATACCGA GCAGTGGACC 
GAGGAGAAGA TTAAGTACGA GGTAGAGCTC TGGCGTTTGT CCCAGAAGCA CGCCAAGGGC 
TTGTCCCTGC GAACGGTGGT GGCCTATGGA GAACACTCCG CCCTGCCCTA CTACATCTCC 
AGCAATGTGA CCAACATCGA GGTCTCCGAC CAGAGTCTGC TGGTCATCGA GTCTGGGGGT 
CAGTATCTGG AGGGCACCAC GGACGTGTCG CGCACCTTCA TTTTTGGTGA GCCCACGCAC 
GAGATGAAAA AGGCCTACAC GAATGTTCTG GCTGGAATTC TGCATCTGGC ACAACTCAAG 
TTTCCGTCCG ATCTGAAGCC CTCCGAGGTG GACGCCTTGG TGCGCAGCAT GGTTTGGAAG 
GACATGACGG ACTTCCCGCA GGCAACAGGT CATGGCATTG GATCCTTTGG ATCTGTGGAA 
GAACCTCCTA TATCAGTTTC CTATGGCAAA AATAGCAGTT TTCACTTCAA GCAGGGCTAC 
TTCTTCTCTA GCGAATCCGG CTACTACAAA CGCGATGACT TTGGCGTTCG TCTGAAGAAC 
GTGCTCGAGG TTGTGGACAC TGGCCATACA CATCCCAGTG GAGCACGCTT CCTTGCATTC 
CGGGACGTCA CCATGGTGCC GTATGAGCCC AAACTGATCG ACAGTACGCT CCTGAGTGCC 
GCGGAGAAGC GTCTCCTAAA TGAGTACAAT GCCAAGATCC GGAACGATAT TGGGGACGAA 
CTGAAGCGAC TGGGCAACAT GCGGGCCTTC TACTGGATGA TGAACCAAAC GCGTCACATT 
CGGGAGTATC TGCCTGAGGA TGAGTACCGC TCTGCGACCG GAGCAGGAAG CTGTGGTCAC 
TCCGCCAGTC CCCTGATACT ACTGGGACTG GGACTAATGG TTATCCTTTC TGCGATTATA 
CCCTCGCGAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650221.1
CDS	248..2362
Translation	MTTAKWIWRLALSILLVVGFVAAEKPTGYHREICQYRKGKHIQPMSVYHNRLMAMREQMQIRATLQGPEIYGYILPSTDEHLNQEVAARDQRLRYLSGFSGVRAFAAVTSHGAAIWVENRYAQQADGELECDWEIYLTSGNVSVADWLGSHVHIDKRVGADPHLVPHSLWVQWERELEDKFLKLVRINTNLVDHIWGDERPEMPKNQVIKVHEKHFAGESWQDKVKELRRRLAHLGCDAMVVTSLTEIAYLLNIRGTDIPYTPVIKSYAVISRDDIFFYVDHSKISLGIDLHLRTDCFNEDCVKIKEYNQIWSDIRTYAQIWKRVLVPAPCVQDLGASEAIYTSMPGKIVVWEISPIIFMRAQKNSDEQAGMRRAHIRDGAAICEAMSNMETRFHTEQWTEEKIKYEVELWRLSQKHAKGLSLRTVVAYGEHSALPYYISSNVTNIEVSDQSLLVIESGGQYLEGTTDVSRTFIFGEPTHEMKKAYTNVLAGILHLAQLKFPSDLKPSEVDALVRSMVWKDMTDFPQATGHGIGSFGSVEEPPISVSYGKNSSFHFKQGYFFSSESGYYKRDDFGVRLKNVLEVVDTGHTHPSGARFLAFRDVTMVPYEPKLIDSTLLSAAEKRLLNEYNAKIRNDIGDELKRLGNMRAFYWMMNQTRHIREYLPEDEYRSATGAGSCGHSASPLILLGLGLMVILSAIIPSRS

Target ORF information:

RefSeq Version	NM_141964.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG6225), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141964.3

ORF Insert Sequence:

ATGACGACAG CCAAATGGAT TTGGCGCTTG GCGCTGAGCA TCTTGCTGGT CGTGGGCTTT 
GTGGCTGCGG AAAAGCCGAC TGGCTATCAT CGCGAGATTT GCCAATACCG CAAGGGCAAA 
CATATCCAGC CCATGAGTGT TTATCACAAT CGCCTGATGG CGATGCGCGA ACAAATGCAG 
ATTAGAGCCA CGCTGCAGGG ACCGGAGATC TATGGCTACA TACTGCCCTC CACGGATGAG 
CATCTCAACC AGGAGGTGGC AGCGCGAGAT CAGAGATTGC GATATCTGTC CGGCTTCTCT 
GGAGTGCGTG CCTTTGCTGC CGTGACCAGT CACGGAGCAG CCATTTGGGT GGAGAACAGA 
TATGCCCAGC AGGCCGATGG AGAGCTGGAG TGCGACTGGG AGATCTATCT GACCAGCGGG 
AATGTATCAG TGGCCGACTG GTTGGGGAGC CATGTTCACA TCGACAAGCG AGTGGGTGCC 
GATCCCCATC TGGTGCCGCA CTCCCTTTGG GTTCAGTGGG AGCGAGAGCT GGAGGACAAG 
TTCCTTAAGC TGGTCAGGAT CAATACCAAT CTGGTGGATC ACATTTGGGG CGATGAGCGA 
CCGGAGATGC CCAAGAACCA GGTGATAAAA GTGCACGAGA AGCACTTCGC CGGCGAGAGT 
TGGCAGGACA AGGTCAAGGA GTTGAGAAGG CGACTGGCGC ATCTGGGCTG CGATGCCATG 
GTGGTCACTT CGCTCACCGA GATTGCCTAC TTGCTAAACA TTCGGGGCAC AGACATTCCG 
TATACTCCTG TGATTAAGTC ATACGCCGTG ATCAGTCGGG ATGATATATT CTTCTATGTG 
GACCACTCAA AGATTAGTCT GGGCATTGAC CTTCATCTCC GCACAGACTG CTTCAATGAA 
GACTGCGTGA AAATCAAAGA GTACAATCAG ATCTGGAGTG ACATTCGTAC CTATGCTCAG 
ATCTGGAAGC GCGTCCTTGT ACCCGCTCCT TGTGTACAGG ATCTTGGAGC GTCGGAGGCG 
ATATACACCT CCATGCCAGG CAAAATTGTC GTCTGGGAGA TCTCACCCAT TATCTTTATG 
CGGGCACAGA AAAACTCCGA TGAACAGGCC GGTATGAGAA GGGCACACAT CCGGGATGGC 
GCCGCCATTT GTGAGGCCAT GAGCAACATG GAGACTAGGT TCCATACCGA GCAGTGGACC 
GAGGAGAAGA TTAAGTACGA GGTAGAGCTC TGGCGTTTGT CCCAGAAGCA CGCCAAGGGC 
TTGTCCCTGC GAACGGTGGT GGCCTATGGA GAACACTCCG CCCTGCCCTA CTACATCTCC 
AGCAATGTGA CCAACATCGA GGTCTCCGAC CAGAGTCTGC TGGTCATCGA GTCTGGGGGT 
CAGTATCTGG AGGGCACCAC GGACGTGTCG CGCACCTTCA TTTTTGGTGA GCCCACGCAC 
GAGATGAAAA AGGCCTACAC GAATGTTCTG GCTGGAATTC TGCATCTGGC ACAACTCAAG 
TTTCCGTCCG ATCTGAAGCC CTCCGAGGTG GACGCCTTGG TGCGCAGCAT GGTTTGGAAG 
GACATGACGG ACTTCCCGCA GGCAACAGGT CATGGCATTG GATCCTTTGG ATCTGTGGAA 
GAACCTCCTA TATCAGTTTC CTATGGCAAA AATAGCAGTT TTCACTTCAA GCAGGGCTAC 
TTCTTCTCTA GCGAATCCGG CTACTACAAA CGCGATGACT TTGGCGTTCG TCTGAAGAAC 
GTGCTCGAGG TTGTGGACAC TGGCCATACA CATCCCAGTG GAGCACGCTT CCTTGCATTC 
CGGGACGTCA CCATGGTGCC GTATGAGCCC AAACTGATCG ACAGTACGCT CCTGAGTGCC 
GCGGAGAAGC GTCTCCTAAA TGAGTACAAT GCCAAGATCC GGAACGATAT TGGGGACGAA 
CTGAAGCGAC TGGGCAACAT GCGGGCCTTC TACTGGATGA TGAACCAAAC GCGTCACATT 
CGGGAGTATC TGCCTGAGGA TGAGTACCGC TCTGCGACCG GAGCAGGAAG CTGTGGTCAC 
TCCGCCAGTC CCCTGATACT ACTGGGACTG GGACTAATGG TTATCCTTTC TGCGATTATA 
CCCTCGCGAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG6225 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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