Dtg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dtg
Entrez Gene ID	41558
Full Name	Dpp target gene
Synonyms	CG6234,Dmel\CG6234
General protein information	Preferred Names Dpp target gene Names CG6234 gene product from transcript CG6234-RA CG6234-PA Dtg-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87C3-87C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_141963.3	NP_650220.2	Dpp target gene

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results indicate that these genes exhibited temporal and spatially restricted patterns of expression in developing embryos, and moreover, their transcripts were detected in cells that undergo morphological changes during the gastrulation stage
Title: Identification of genes expressed during Drosophila melanogaster gastrulation by using subtractive hybridization.

The following Dtg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dtg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04708	NM_141963.3 Latest version!	Drosophila melanogaster Dpp target gene (Dtg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dtg ( NM_141963.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650220.2 Drosophila melanogaster Dpp target gene (Dtg), mRNA.

Price & Availability↑

CloneID	OFa04708
Clone ID Related Accession (Same CDS sequence)	NM_141963.3
Accession Version	NM_141963.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dpp target gene
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141963.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAGTA TTTCTAAGAG CCGCGCCAGC AGCTGCTCCT CCAGAAAGAG GCTGCATCTG 
CAACCGGGAT CGCTGCTCTG CGTGATCGTC TTCTGCCTGC TGGCTTCGTG CTCGGCTCAG 
CCAATCGAGT CAACCTCATT GCCTGCAGAA GTGGACGATG TGGAGGGTAC CACAATACTT 
CCCATCCAGT TGGAGGAGAG TTCACCAACA CCAGAACCCA TTATCAAGAA TGAAACAGCT 
ACTGCTCTTC CTGTCGTGGA ACTCAAAGTA GCTGAATCTA ATCCTGTAGA TCCTGTTGAG 
GCTACGGATA ATTTTGTATC GGTTTACGGC GGAAATCTGG AGCAGGATAT AAAGAACGAC 
GTGAACAGCG AAGTGGAAGA TGCGCCCTCT CCGGTGGAAC CAATCATCGA GGAAGTTCAA 
AAAGTGGCAG AAGCTGAACT TGAAAATCAT GAAATTAAGC TTGCGGTCCA AAGTGCTCGG 
GAGCCCAAAT CCCTGGATGC TGAAGAGGAG TCAAAGACAA TCGATGAAGA TCGCTTGGAA 
AATCAAGATA AAGCGGAGAC AGTAACGGAT AACATTGCCG TTTCCAGTGA GCAGATGGAG 
AGCCACATCA GACCATTACC CAACGTGACC AGTGACCTGG TTTCCGTGAT TCTCAACGAA 
GACAAAGCCA TCACTGAGCA ACCGATCACC AAAACGAACC TTCTGGCTTT GGAGCAGGAG 
CACGAGAAAT TCGAGGAAAT TGATGAAAGT ACTACAGTGC CAGTTTATGA GGAGCCCAAG 
CACCAAGTCA CCAAGAAACA AGGCGTTGAG GACCCATCTA CCGTTGAAAT TCCACCTGAG 
GTATCTGACG AACAACACTT TGATCCTTCC GAGGAACCCC AGACTGTTCG CGACGAACGT 
AATCTTGAAG AGCACGACGA CAACCAGAAT GAGATAAATG AGAACATCAT TGAAGGGGAG 
GAGGCTCCAG CCAAAACGTC AACCACCGCA GGTCCCTTGG TCACCGTGGA ACCCACTAAA 
TCGATTACCG AGCCAAATGA GGAGATCGAG AAAAAGGCGG AAGCTGAGGC TAAGGCGGAA 
ATGGAGGAGC AAGTGGAGGT CAACACTCCT TCTGTAGACG CAACTGTGGT GGCAGCGGAT 
CCTGATGAAG CACAAGATGA AGTAATCGTT GCTGAAACAA CAGTAGAAGC TATCACAGAA 
GCTGCCAAGA TTGTTGTGGA GGAGGAACCC AAGGAAGATG TTTCAGTTCA ACAGGAGCAT 
CTAAAGGAAC AAAGAGTCCA GACAGTTTCC GAGTCCAACC GTCAAGCAAG TCCCACCGAG 
GAACCAATCA AAGAAGTGAT CTTTCCAAAA GGTGATGATG AATCCCCGGT AGAAGGGGAT 
AGCTCCGAAT CGGATGAGAA ACCAACTGAG AGAGCAATAG TGGATGACGA CTCCTCGGAG 
GAGCAAAACG AGGACGTTGT GGATGAGGGA AAGTCCTCTT CCGACGACAG CACCGCCACT 
CCGCTGGTCT CCTATCCCCC ACACGATGCT GGCCAAACCT TCGATAGCAA CTCGGCTGAC 
GAACACTCGG CCCAGCTGTC CGGAGCCACC AACTACCGGT CCACCCTGAT CATAGCCCTG 
TGCTCCGGCA CAGCAGTGAT CTTCATCGTG GCCTCGCTGG TCATCTTCGT GGTTTCCTTC 
CAACGGCAGC ACGGCACCTT GGACATTGAG ATGCAGGAAC AGCGTTTGGG CAAGGATATT 
CAGGAAGAGG ACAATGTCCA GATGAAGCTG CTCGATGTGG ATCTCTCGTC GCCCGTCATT 
CTGCCGATGG GCAACGAGGA GACCGATGAA TGCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650220.2
CDS	302..2140
Translation	MASISKSRASSCSSRKRLHLQPGSLLCVIVFCLLASCSAQPIESTSLPAEVDDVEGTTILPIQLEESSPTPEPIIKNETATALPVVELKVAESNPVDPVEATDNFVSVYGGNLEQDIKNDVNSEVEDAPSPVEPIIEEVQKVAEAELENHEIKLAVQSAREPKSLDAEEESKTIDEDRLENQDKAETVTDNIAVSSEQMESHIRPLPNVTSDLVSVILNEDKAITEQPITKTNLLALEQEHEKFEEIDESTTVPVYEEPKHQVTKKQGVEDPSTVEIPPEVSDEQHFDPSEEPQTVRDERNLEEHDDNQNEINENIIEGEEAPAKTSTTAGPLVTVEPTKSITEPNEEIEKKAEAEAKAEMEEQVEVNTPSVDATVVAADPDEAQDEVIVAETTVEAITEAAKIVVEEEPKEDVSVQQEHLKEQRVQTVSESNRQASPTEEPIKEVIFPKGDDESPVEGDSSESDEKPTERAIVDDDSSEEQNEDVVDEGKSSSDDSTATPLVSYPPHDAGQTFDSNSADEHSAQLSGATNYRSTLIIALCSGTAVIFIVASLVIFVVSFQRQHGTLDIEMQEQRLGKDIQEEDNVQMKLLDVDLSSPVILPMGNEETDECL

Target ORF information:

RefSeq Version	NM_141963.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dpp target gene (Dtg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141963.3

ORF Insert Sequence:

ATGGCCAGTA TTTCTAAGAG CCGCGCCAGC AGCTGCTCCT CCAGAAAGAG GCTGCATCTG 
CAACCGGGAT CGCTGCTCTG CGTGATCGTC TTCTGCCTGC TGGCTTCGTG CTCGGCTCAG 
CCAATCGAGT CAACCTCATT GCCTGCAGAA GTGGACGATG TGGAGGGTAC CACAATACTT 
CCCATCCAGT TGGAGGAGAG TTCACCAACA CCAGAACCCA TTATCAAGAA TGAAACAGCT 
ACTGCTCTTC CTGTCGTGGA ACTCAAAGTA GCTGAATCTA ATCCTGTAGA TCCTGTTGAG 
GCTACGGATA ATTTTGTATC GGTTTACGGC GGAAATCTGG AGCAGGATAT AAAGAACGAC 
GTGAACAGCG AAGTGGAAGA TGCGCCCTCT CCGGTGGAAC CAATCATCGA GGAAGTTCAA 
AAAGTGGCAG AAGCTGAACT TGAAAATCAT GAAATTAAGC TTGCGGTCCA AAGTGCTCGG 
GAGCCCAAAT CCCTGGATGC TGAAGAGGAG TCAAAGACAA TCGATGAAGA TCGCTTGGAA 
AATCAAGATA AAGCGGAGAC AGTAACGGAT AACATTGCCG TTTCCAGTGA GCAGATGGAG 
AGCCACATCA GACCATTACC CAACGTGACC AGTGACCTGG TTTCCGTGAT TCTCAACGAA 
GACAAAGCCA TCACTGAGCA ACCGATCACC AAAACGAACC TTCTGGCTTT GGAGCAGGAG 
CACGAGAAAT TCGAGGAAAT TGATGAAAGT ACTACAGTGC CAGTTTATGA GGAGCCCAAG 
CACCAAGTCA CCAAGAAACA AGGCGTTGAG GACCCATCTA CCGTTGAAAT TCCACCTGAG 
GTATCTGACG AACAACACTT TGATCCTTCC GAGGAACCCC AGACTGTTCG CGACGAACGT 
AATCTTGAAG AGCACGACGA CAACCAGAAT GAGATAAATG AGAACATCAT TGAAGGGGAG 
GAGGCTCCAG CCAAAACGTC AACCACCGCA GGTCCCTTGG TCACCGTGGA ACCCACTAAA 
TCGATTACCG AGCCAAATGA GGAGATCGAG AAAAAGGCGG AAGCTGAGGC TAAGGCGGAA 
ATGGAGGAGC AAGTGGAGGT CAACACTCCT TCTGTAGACG CAACTGTGGT GGCAGCGGAT 
CCTGATGAAG CACAAGATGA AGTAATCGTT GCTGAAACAA CAGTAGAAGC TATCACAGAA 
GCTGCCAAGA TTGTTGTGGA GGAGGAACCC AAGGAAGATG TTTCAGTTCA ACAGGAGCAT 
CTAAAGGAAC AAAGAGTCCA GACAGTTTCC GAGTCCAACC GTCAAGCAAG TCCCACCGAG 
GAACCAATCA AAGAAGTGAT CTTTCCAAAA GGTGATGATG AATCCCCGGT AGAAGGGGAT 
AGCTCCGAAT CGGATGAGAA ACCAACTGAG AGAGCAATAG TGGATGACGA CTCCTCGGAG 
GAGCAAAACG AGGACGTTGT GGATGAGGGA AAGTCCTCTT CCGACGACAG CACCGCCACT 
CCGCTGGTCT CCTATCCCCC ACACGATGCT GGCCAAACCT TCGATAGCAA CTCGGCTGAC 
GAACACTCGG CCCAGCTGTC CGGAGCCACC AACTACCGGT CCACCCTGAT CATAGCCCTG 
TGCTCCGGCA CAGCAGTGAT CTTCATCGTG GCCTCGCTGG TCATCTTCGT GGTTTCCTTC 
CAACGGCAGC ACGGCACCTT GGACATTGAG ATGCAGGAAC AGCGTTTGGG CAAGGATATT 
CAGGAAGAGG ACAATGTCCA GATGAAGCTG CTCGATGTGG ATCTCTCGTC GCCCGTCATT 
CTGCCGATGG GCAACGAGGA GACCGATGAA TGCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dtg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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