Vha55 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vha55
Entrez Gene ID	41550
Full Name	Vacuolar H[+]-ATPase 55kD subunit
Synonyms	1208/13,ATP6V1B1,CG17369,Dmel\CG17369,SzA,V-ATPase,VAT-2,l(3)87Ca,l(3)SzA,l(3)j2E9,vha55
General protein information	Preferred Names Vacuolar H[+]-ATPase 55kD subunit Names CG17369 gene product from transcript CG17369-RB B subunit B subunit of V-ATPase CG17369-PA CG17369-PB CG17369-PC V-ATPase B-subunit V-ATPase subunit B V1 B subunit Vha55-PA Vha55-PB Vha55-PC vacuolar ATP synthase subunit B vacuolar ATPase B subunit vacuolar H+-ATPase 55kD B subunit vacuolar H+ATPase subunit B vacuolar H[+]-ATPase vacuolar H[+]-ATPase 55-kDa B subunit vacuolar H[+]-ATPase 55kD B subunit vacuolar H[+]-ATPase 55kD subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87C2-87C3\|3-51.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170126.1	NP_001163597.1	vacuolar H[+]-ATPase 55kD subunit, isoform C
NM_169475.2	NP_731726.1	vacuolar H[+]-ATPase 55kD subunit, isoform A
NM_057560.4	NP_476908.1	vacuolar H[+]-ATPase 55kD subunit, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP6V1B2	NP_001684.2
Pan troglodytes (chimpanzee)	ATP6V1B2	XP_519638.2
Saccharomyces cerevisiae (baker's yeast)	VMA2	NP_009685.3
Arabidopsis thaliana (thale cress)	AT1G20260	NP_173451.5
Oryza sativa (rice)	Os06g0568200	NP_001057902.1
Rattus norvegicus (Norway rat)	Atp6v1b2	NP_476561.1
Caenorhabditis elegans (roundworm)	spe-5	NP_491518.1
Magnaporthe oryzae (rice blast fungus)	MGG_03244	XP_003716730.1
Xenopus tropicalis (tropical clawed frog)	atp6v1b2	NP_001072182.1
	ATP6V1B2	XP_001100869.1
Canis lupus familiaris (dog)	ATP6V1B2	XP_543263.2
Bos taurus (cattle)	ATP6V1B2	NP_788844.2
Mus musculus (house mouse)	Atp6v1b2	NP_031535.2
Arabidopsis thaliana (thale cress)	AT1G76030	NP_177729.1
Gallus gallus (chicken)	ATP6V1B2	XP_424534.3
Drosophila melanogaster (fruit fly)	Vha55	NP_001163597.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002884	XP_312029.3
Schizosaccharomyces pombe (fission yeast)	vma2	NP_594623.1
Neurospora crassa	NCU08515	XP_963165.1
Danio rerio (zebrafish)	atp6v1b2	NP_878299.1
Kluyveromyces lactis	KLLA0D09152g	XP_453470.1
Eremothecium gossypii	AGOS_ADL380W	NP_983716.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vacuolar ATPase (V-ATPase) and the V-ATPase regulator Rabconnectin-3 are required for subcellular tube formation, probably in a step resolving the intermediate compartment into a mature lumen.
Title: Intracellular lumen formation in Drosophila proceeds via a novel subcellular compartment.

Here, mutations underlying five extant lethal alleles of vha55, encoding the B subunit, were identified, including a premature termination codon and two mutations very close to residues thought to participate in the catalytic site of the V-ATPases.
Title: The SzA mutations of the B subunit of the Drosophila vacuolar H+ ATPase identify conserved residues essential for function in fly and yeast.

The following Vha55 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vha55 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04701	NM_169475.2 Latest version!	Drosophila melanogaster vacuolar H[+]-ATPase 55kD subunit, transcript variant A (Vha55), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04701	NM_001170126.1 Latest version!	Drosophila melanogaster vacuolar H[+]-ATPase 55kD subunit, transcript variant C (Vha55), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04701	NM_057560.4 Latest version!	Drosophila melanogaster vacuolar H[+]-ATPase 55kD subunit, transcript variant B (Vha55), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vha55 ( NM_001170126.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163597.1 Drosophila melanogaster vacuolar H[+]-ATPase 55kD subunit, transcript variant C (Vha55), mRNA.

Price & Availability↑

CloneID	OFa04701
Clone ID Related Accession (Same CDS sequence)	NM_001170126.1 , NM_169475.2 , NM_057560.4
Accession Version	NM_001170126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+]-ATPase 55kD subunit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGCCC AACAAGCCCA GCGGGAACAT GTCCTCGCCG TCTCCCGGGA CTTTATCTCC 
CAGCCCCGCT TGACTTACAA GACTGTGTCT GGTGTGAACG GTCCCCTGGT CATTCTCGAT 
GAGGTCAAGT TCCCCAAGTT CGCCGAGATC GTGCAGCTGC GTCTGGCTGA CGGTACTGTG 
CGCTCCGGAC AGGTGCTCGA GGTGAGCGGC TCCAAGGCCG TGGTCCAGGT GTTCGAGGGC 
ACCTCGGGCA TCGATGCCAA GAACACGCTT TGCGAGTTCA CCGGCGACAT TCTGCGCACG 
CCCGTGTCGG AGGATATGTT GGGCCGTGTG TTCAACGGTT CCGGCAAGCC CATCGACAAG 
GGACCCCCAA TTCTGGCTGA GGATTTCCTG GACATCCAGG GCCAGCCCAT CAACCCCTGG 
TCGCGTATCT ACCCCGAGGA GATGATCCAG ACCGGTATCT CGGCCATCGA TGTGATGAAC 
TCGATTGCCC GTGGCCAGAA GATCCCCATC TTCTCCGCCG CCGGTCTGCC CCACAACGAA 
ATCGCCGCCC AGATCTGTCG TCAGGCCGGT CTCGTCAAGC TGCCGGGCAA GTCCGTGCTG 
GATGACCACA CCGACAACTT CGCCATCGTG TTCGCCGCTA TGGGTGTGAA CATGGAGACT 
GCCCGATTCT TCAAGCAGGA CTTCGAGGAG AACGGTTCCA TGGAGAACGT GTGTCTGTTC 
TTGAACTTGG CCAACGATCC GACCATCGAG CGTATCATCA CTCCCCGCCT GGCTTTGACT 
GCCGCCGAGT TCTTGGCCTA CCAGTGCGAG AAGCACGTGC TGGTCATCCT TACCGACATG 
TCCTCGTACG CCGAGGCTCT GCGTGAGGTG TCTGCTGCCC GTGAGGAGGT GCCCGGTCGT 
CGTGGTTTCC CCGGTTACAT GTACACCGAT TTGGCCACCA TCTACGAGCG TGCCGGTCGT 
GTGGAGGGAC GCAACGGATC CATCACCCAG ATCCCCATTC TGACCATGCC TAACGATGAT 
ATTACCCATC CCATTCCCGA TTTGACCGGT TACATTACCG AGGGCCAGAT CTACGTCGAT 
CGTCAGCTGC ACAACAGACA GATCTACCCA CCAGTCAACG TGCTGCCATC GCTGTCGCGT 
TTGATGAAGT CTGCCATCGG CGAGGGCATG ACCCGTAAGG ACCACTCTGA CGTGTCCAAC 
CAGCTGTACG CTTGCTACGC CATCGGCAAG GACGTGCAGG CCATGAAGGC TGTGGTGGGT 
GAGGAGGCCC TGACGCCCGA CGATCTGCTG TATCTGGAGT TCTTGACCAA GTTCGAGAAG 
AACTTCATCT CGCAGGGCAA CTATGAGAAC CGCACTGTCT TCGAATCCCT GGACATTGGC 
TGGCAGCTGC TGCGTATCTT CCCCAAGGAG ATGCTCAAGC GTATCCCGGC CTCCATCCTG 
GCCGAATTCT ACCCTAGGGA CTCGCGCCAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163597.1
CDS	116..1588
Translation	MNAQQAQREHVLAVSRDFISQPRLTYKTVSGVNGPLVILDEVKFPKFAEIVQLRLADGTVRSGQVLEVSGSKAVVQVFEGTSGIDAKNTLCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPPILAEDFLDIQGQPINPWSRIYPEEMIQTGISAIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKLPGKSVLDDHTDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIERIITPRLALTAAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPVNVLPSLSRLMKSAIGEGMTRKDHSDVSNQLYACYAIGKDVQAMKAVVGEEALTPDDLLYLEFLTKFEKNFISQGNYENRTVFESLDIGWQLLRIFPKEMLKRIPASILAEFYPRDSRH

Target ORF information:

RefSeq Version	NM_001170126.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+]-ATPase 55kD subunit, transcript variant C (Vha55), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170126.1

ORF Insert Sequence:

ATGAACGCCC AACAAGCCCA GCGGGAACAT GTCCTCGCCG TCTCCCGGGA CTTTATCTCC 
CAGCCCCGCT TGACTTACAA GACTGTGTCT GGTGTGAACG GTCCCCTGGT CATTCTCGAT 
GAGGTCAAGT TCCCCAAGTT CGCCGAGATC GTGCAGCTGC GTCTGGCTGA CGGTACTGTG 
CGCTCCGGAC AGGTGCTCGA GGTGAGCGGC TCCAAGGCCG TGGTCCAGGT GTTCGAGGGC 
ACCTCGGGCA TCGATGCCAA GAACACGCTT TGCGAGTTCA CCGGCGACAT TCTGCGCACG 
CCCGTGTCGG AGGATATGTT GGGCCGTGTG TTCAACGGTT CCGGCAAGCC CATCGACAAG 
GGACCCCCAA TTCTGGCTGA GGATTTCCTG GACATCCAGG GCCAGCCCAT CAACCCCTGG 
TCGCGTATCT ACCCCGAGGA GATGATCCAG ACCGGTATCT CGGCCATCGA TGTGATGAAC 
TCGATTGCCC GTGGCCAGAA GATCCCCATC TTCTCCGCCG CCGGTCTGCC CCACAACGAA 
ATCGCCGCCC AGATCTGTCG TCAGGCCGGT CTCGTCAAGC TGCCGGGCAA GTCCGTGCTG 
GATGACCACA CCGACAACTT CGCCATCGTG TTCGCCGCTA TGGGTGTGAA CATGGAGACT 
GCCCGATTCT TCAAGCAGGA CTTCGAGGAG AACGGTTCCA TGGAGAACGT GTGTCTGTTC 
TTGAACTTGG CCAACGATCC GACCATCGAG CGTATCATCA CTCCCCGCCT GGCTTTGACT 
GCCGCCGAGT TCTTGGCCTA CCAGTGCGAG AAGCACGTGC TGGTCATCCT TACCGACATG 
TCCTCGTACG CCGAGGCTCT GCGTGAGGTG TCTGCTGCCC GTGAGGAGGT GCCCGGTCGT 
CGTGGTTTCC CCGGTTACAT GTACACCGAT TTGGCCACCA TCTACGAGCG TGCCGGTCGT 
GTGGAGGGAC GCAACGGATC CATCACCCAG ATCCCCATTC TGACCATGCC TAACGATGAT 
ATTACCCATC CCATTCCCGA TTTGACCGGT TACATTACCG AGGGCCAGAT CTACGTCGAT 
CGTCAGCTGC ACAACAGACA GATCTACCCA CCAGTCAACG TGCTGCCATC GCTGTCGCGT 
TTGATGAAGT CTGCCATCGG CGAGGGCATG ACCCGTAAGG ACCACTCTGA CGTGTCCAAC 
CAGCTGTACG CTTGCTACGC CATCGGCAAG GACGTGCAGG CCATGAAGGC TGTGGTGGGT 
GAGGAGGCCC TGACGCCCGA CGATCTGCTG TATCTGGAGT TCTTGACCAA GTTCGAGAAG 
AACTTCATCT CGCAGGGCAA CTATGAGAAC CGCACTGTCT TCGAATCCCT GGACATTGGC 
TGGCAGCTGC TGCGTATCTT CCCCAAGGAG ATGCTCAAGC GTATCCCGGC CTCCATCCTG 
GCCGAATTCT ACCCTAGGGA CTCGCGCCAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha55 ( NM_169475.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731726.1 Drosophila melanogaster vacuolar H[+]-ATPase 55kD subunit, transcript variant A (Vha55), mRNA.

Price & Availability↑

CloneID	OFa04701
Clone ID Related Accession (Same CDS sequence)	NM_001170126.1 , NM_169475.2 , NM_057560.4
Accession Version	NM_169475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+]-ATPase 55kD subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_169475.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGCCC AACAAGCCCA GCGGGAACAT GTCCTCGCCG TCTCCCGGGA CTTTATCTCC 
CAGCCCCGCT TGACTTACAA GACTGTGTCT GGTGTGAACG GTCCCCTGGT CATTCTCGAT 
GAGGTCAAGT TCCCCAAGTT CGCCGAGATC GTGCAGCTGC GTCTGGCTGA CGGTACTGTG 
CGCTCCGGAC AGGTGCTCGA GGTGAGCGGC TCCAAGGCCG TGGTCCAGGT GTTCGAGGGC 
ACCTCGGGCA TCGATGCCAA GAACACGCTT TGCGAGTTCA CCGGCGACAT TCTGCGCACG 
CCCGTGTCGG AGGATATGTT GGGCCGTGTG TTCAACGGTT CCGGCAAGCC CATCGACAAG 
GGACCCCCAA TTCTGGCTGA GGATTTCCTG GACATCCAGG GCCAGCCCAT CAACCCCTGG 
TCGCGTATCT ACCCCGAGGA GATGATCCAG ACCGGTATCT CGGCCATCGA TGTGATGAAC 
TCGATTGCCC GTGGCCAGAA GATCCCCATC TTCTCCGCCG CCGGTCTGCC CCACAACGAA 
ATCGCCGCCC AGATCTGTCG TCAGGCCGGT CTCGTCAAGC TGCCGGGCAA GTCCGTGCTG 
GATGACCACA CCGACAACTT CGCCATCGTG TTCGCCGCTA TGGGTGTGAA CATGGAGACT 
GCCCGATTCT TCAAGCAGGA CTTCGAGGAG AACGGTTCCA TGGAGAACGT GTGTCTGTTC 
TTGAACTTGG CCAACGATCC GACCATCGAG CGTATCATCA CTCCCCGCCT GGCTTTGACT 
GCCGCCGAGT TCTTGGCCTA CCAGTGCGAG AAGCACGTGC TGGTCATCCT TACCGACATG 
TCCTCGTACG CCGAGGCTCT GCGTGAGGTG TCTGCTGCCC GTGAGGAGGT GCCCGGTCGT 
CGTGGTTTCC CCGGTTACAT GTACACCGAT TTGGCCACCA TCTACGAGCG TGCCGGTCGT 
GTGGAGGGAC GCAACGGATC CATCACCCAG ATCCCCATTC TGACCATGCC TAACGATGAT 
ATTACCCATC CCATTCCCGA TTTGACCGGT TACATTACCG AGGGCCAGAT CTACGTCGAT 
CGTCAGCTGC ACAACAGACA GATCTACCCA CCAGTCAACG TGCTGCCATC GCTGTCGCGT 
TTGATGAAGT CTGCCATCGG CGAGGGCATG ACCCGTAAGG ACCACTCTGA CGTGTCCAAC 
CAGCTGTACG CTTGCTACGC CATCGGCAAG GACGTGCAGG CCATGAAGGC TGTGGTGGGT 
GAGGAGGCCC TGACGCCCGA CGATCTGCTG TATCTGGAGT TCTTGACCAA GTTCGAGAAG 
AACTTCATCT CGCAGGGCAA CTATGAGAAC CGCACTGTCT TCGAATCCCT GGACATTGGC 
TGGCAGCTGC TGCGTATCTT CCCCAAGGAG ATGCTCAAGC GTATCCCGGC CTCCATCCTG 
GCCGAATTCT ACCCTAGGGA CTCGCGCCAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731726.1
CDS	181..1653
Translation	MNAQQAQREHVLAVSRDFISQPRLTYKTVSGVNGPLVILDEVKFPKFAEIVQLRLADGTVRSGQVLEVSGSKAVVQVFEGTSGIDAKNTLCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPPILAEDFLDIQGQPINPWSRIYPEEMIQTGISAIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKLPGKSVLDDHTDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIERIITPRLALTAAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPVNVLPSLSRLMKSAIGEGMTRKDHSDVSNQLYACYAIGKDVQAMKAVVGEEALTPDDLLYLEFLTKFEKNFISQGNYENRTVFESLDIGWQLLRIFPKEMLKRIPASILAEFYPRDSRH

Target ORF information:

RefSeq Version	NM_169475.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+]-ATPase 55kD subunit, transcript variant A (Vha55), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169475.2

ORF Insert Sequence:

ATGAACGCCC AACAAGCCCA GCGGGAACAT GTCCTCGCCG TCTCCCGGGA CTTTATCTCC 
CAGCCCCGCT TGACTTACAA GACTGTGTCT GGTGTGAACG GTCCCCTGGT CATTCTCGAT 
GAGGTCAAGT TCCCCAAGTT CGCCGAGATC GTGCAGCTGC GTCTGGCTGA CGGTACTGTG 
CGCTCCGGAC AGGTGCTCGA GGTGAGCGGC TCCAAGGCCG TGGTCCAGGT GTTCGAGGGC 
ACCTCGGGCA TCGATGCCAA GAACACGCTT TGCGAGTTCA CCGGCGACAT TCTGCGCACG 
CCCGTGTCGG AGGATATGTT GGGCCGTGTG TTCAACGGTT CCGGCAAGCC CATCGACAAG 
GGACCCCCAA TTCTGGCTGA GGATTTCCTG GACATCCAGG GCCAGCCCAT CAACCCCTGG 
TCGCGTATCT ACCCCGAGGA GATGATCCAG ACCGGTATCT CGGCCATCGA TGTGATGAAC 
TCGATTGCCC GTGGCCAGAA GATCCCCATC TTCTCCGCCG CCGGTCTGCC CCACAACGAA 
ATCGCCGCCC AGATCTGTCG TCAGGCCGGT CTCGTCAAGC TGCCGGGCAA GTCCGTGCTG 
GATGACCACA CCGACAACTT CGCCATCGTG TTCGCCGCTA TGGGTGTGAA CATGGAGACT 
GCCCGATTCT TCAAGCAGGA CTTCGAGGAG AACGGTTCCA TGGAGAACGT GTGTCTGTTC 
TTGAACTTGG CCAACGATCC GACCATCGAG CGTATCATCA CTCCCCGCCT GGCTTTGACT 
GCCGCCGAGT TCTTGGCCTA CCAGTGCGAG AAGCACGTGC TGGTCATCCT TACCGACATG 
TCCTCGTACG CCGAGGCTCT GCGTGAGGTG TCTGCTGCCC GTGAGGAGGT GCCCGGTCGT 
CGTGGTTTCC CCGGTTACAT GTACACCGAT TTGGCCACCA TCTACGAGCG TGCCGGTCGT 
GTGGAGGGAC GCAACGGATC CATCACCCAG ATCCCCATTC TGACCATGCC TAACGATGAT 
ATTACCCATC CCATTCCCGA TTTGACCGGT TACATTACCG AGGGCCAGAT CTACGTCGAT 
CGTCAGCTGC ACAACAGACA GATCTACCCA CCAGTCAACG TGCTGCCATC GCTGTCGCGT 
TTGATGAAGT CTGCCATCGG CGAGGGCATG ACCCGTAAGG ACCACTCTGA CGTGTCCAAC 
CAGCTGTACG CTTGCTACGC CATCGGCAAG GACGTGCAGG CCATGAAGGC TGTGGTGGGT 
GAGGAGGCCC TGACGCCCGA CGATCTGCTG TATCTGGAGT TCTTGACCAA GTTCGAGAAG 
AACTTCATCT CGCAGGGCAA CTATGAGAAC CGCACTGTCT TCGAATCCCT GGACATTGGC 
TGGCAGCTGC TGCGTATCTT CCCCAAGGAG ATGCTCAAGC GTATCCCGGC CTCCATCCTG 
GCCGAATTCT ACCCTAGGGA CTCGCGCCAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha55 ( NM_057560.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476908.1 Drosophila melanogaster vacuolar H[+]-ATPase 55kD subunit, transcript variant B (Vha55), mRNA.

Price & Availability↑

CloneID	OFa04701
Clone ID Related Accession (Same CDS sequence)	NM_001170126.1 , NM_169475.2 , NM_057560.4
Accession Version	NM_057560.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+]-ATPase 55kD subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_057560.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGCCC AACAAGCCCA GCGGGAACAT GTCCTCGCCG TCTCCCGGGA CTTTATCTCC 
CAGCCCCGCT TGACTTACAA GACTGTGTCT GGTGTGAACG GTCCCCTGGT CATTCTCGAT 
GAGGTCAAGT TCCCCAAGTT CGCCGAGATC GTGCAGCTGC GTCTGGCTGA CGGTACTGTG 
CGCTCCGGAC AGGTGCTCGA GGTGAGCGGC TCCAAGGCCG TGGTCCAGGT GTTCGAGGGC 
ACCTCGGGCA TCGATGCCAA GAACACGCTT TGCGAGTTCA CCGGCGACAT TCTGCGCACG 
CCCGTGTCGG AGGATATGTT GGGCCGTGTG TTCAACGGTT CCGGCAAGCC CATCGACAAG 
GGACCCCCAA TTCTGGCTGA GGATTTCCTG GACATCCAGG GCCAGCCCAT CAACCCCTGG 
TCGCGTATCT ACCCCGAGGA GATGATCCAG ACCGGTATCT CGGCCATCGA TGTGATGAAC 
TCGATTGCCC GTGGCCAGAA GATCCCCATC TTCTCCGCCG CCGGTCTGCC CCACAACGAA 
ATCGCCGCCC AGATCTGTCG TCAGGCCGGT CTCGTCAAGC TGCCGGGCAA GTCCGTGCTG 
GATGACCACA CCGACAACTT CGCCATCGTG TTCGCCGCTA TGGGTGTGAA CATGGAGACT 
GCCCGATTCT TCAAGCAGGA CTTCGAGGAG AACGGTTCCA TGGAGAACGT GTGTCTGTTC 
TTGAACTTGG CCAACGATCC GACCATCGAG CGTATCATCA CTCCCCGCCT GGCTTTGACT 
GCCGCCGAGT TCTTGGCCTA CCAGTGCGAG AAGCACGTGC TGGTCATCCT TACCGACATG 
TCCTCGTACG CCGAGGCTCT GCGTGAGGTG TCTGCTGCCC GTGAGGAGGT GCCCGGTCGT 
CGTGGTTTCC CCGGTTACAT GTACACCGAT TTGGCCACCA TCTACGAGCG TGCCGGTCGT 
GTGGAGGGAC GCAACGGATC CATCACCCAG ATCCCCATTC TGACCATGCC TAACGATGAT 
ATTACCCATC CCATTCCCGA TTTGACCGGT TACATTACCG AGGGCCAGAT CTACGTCGAT 
CGTCAGCTGC ACAACAGACA GATCTACCCA CCAGTCAACG TGCTGCCATC GCTGTCGCGT 
TTGATGAAGT CTGCCATCGG CGAGGGCATG ACCCGTAAGG ACCACTCTGA CGTGTCCAAC 
CAGCTGTACG CTTGCTACGC CATCGGCAAG GACGTGCAGG CCATGAAGGC TGTGGTGGGT 
GAGGAGGCCC TGACGCCCGA CGATCTGCTG TATCTGGAGT TCTTGACCAA GTTCGAGAAG 
AACTTCATCT CGCAGGGCAA CTATGAGAAC CGCACTGTCT TCGAATCCCT GGACATTGGC 
TGGCAGCTGC TGCGTATCTT CCCCAAGGAG ATGCTCAAGC GTATCCCGGC CTCCATCCTG 
GCCGAATTCT ACCCTAGGGA CTCGCGCCAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476908.1
CDS	245..1717
Translation	MNAQQAQREHVLAVSRDFISQPRLTYKTVSGVNGPLVILDEVKFPKFAEIVQLRLADGTVRSGQVLEVSGSKAVVQVFEGTSGIDAKNTLCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPPILAEDFLDIQGQPINPWSRIYPEEMIQTGISAIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKLPGKSVLDDHTDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIERIITPRLALTAAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPVNVLPSLSRLMKSAIGEGMTRKDHSDVSNQLYACYAIGKDVQAMKAVVGEEALTPDDLLYLEFLTKFEKNFISQGNYENRTVFESLDIGWQLLRIFPKEMLKRIPASILAEFYPRDSRH

Target ORF information:

RefSeq Version	NM_057560.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+]-ATPase 55kD subunit, transcript variant B (Vha55), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057560.4

ORF Insert Sequence:

ATGAACGCCC AACAAGCCCA GCGGGAACAT GTCCTCGCCG TCTCCCGGGA CTTTATCTCC 
CAGCCCCGCT TGACTTACAA GACTGTGTCT GGTGTGAACG GTCCCCTGGT CATTCTCGAT 
GAGGTCAAGT TCCCCAAGTT CGCCGAGATC GTGCAGCTGC GTCTGGCTGA CGGTACTGTG 
CGCTCCGGAC AGGTGCTCGA GGTGAGCGGC TCCAAGGCCG TGGTCCAGGT GTTCGAGGGC 
ACCTCGGGCA TCGATGCCAA GAACACGCTT TGCGAGTTCA CCGGCGACAT TCTGCGCACG 
CCCGTGTCGG AGGATATGTT GGGCCGTGTG TTCAACGGTT CCGGCAAGCC CATCGACAAG 
GGACCCCCAA TTCTGGCTGA GGATTTCCTG GACATCCAGG GCCAGCCCAT CAACCCCTGG 
TCGCGTATCT ACCCCGAGGA GATGATCCAG ACCGGTATCT CGGCCATCGA TGTGATGAAC 
TCGATTGCCC GTGGCCAGAA GATCCCCATC TTCTCCGCCG CCGGTCTGCC CCACAACGAA 
ATCGCCGCCC AGATCTGTCG TCAGGCCGGT CTCGTCAAGC TGCCGGGCAA GTCCGTGCTG 
GATGACCACA CCGACAACTT CGCCATCGTG TTCGCCGCTA TGGGTGTGAA CATGGAGACT 
GCCCGATTCT TCAAGCAGGA CTTCGAGGAG AACGGTTCCA TGGAGAACGT GTGTCTGTTC 
TTGAACTTGG CCAACGATCC GACCATCGAG CGTATCATCA CTCCCCGCCT GGCTTTGACT 
GCCGCCGAGT TCTTGGCCTA CCAGTGCGAG AAGCACGTGC TGGTCATCCT TACCGACATG 
TCCTCGTACG CCGAGGCTCT GCGTGAGGTG TCTGCTGCCC GTGAGGAGGT GCCCGGTCGT 
CGTGGTTTCC CCGGTTACAT GTACACCGAT TTGGCCACCA TCTACGAGCG TGCCGGTCGT 
GTGGAGGGAC GCAACGGATC CATCACCCAG ATCCCCATTC TGACCATGCC TAACGATGAT 
ATTACCCATC CCATTCCCGA TTTGACCGGT TACATTACCG AGGGCCAGAT CTACGTCGAT 
CGTCAGCTGC ACAACAGACA GATCTACCCA CCAGTCAACG TGCTGCCATC GCTGTCGCGT 
TTGATGAAGT CTGCCATCGG CGAGGGCATG ACCCGTAAGG ACCACTCTGA CGTGTCCAAC 
CAGCTGTACG CTTGCTACGC CATCGGCAAG GACGTGCAGG CCATGAAGGC TGTGGTGGGT 
GAGGAGGCCC TGACGCCCGA CGATCTGCTG TATCTGGAGT TCTTGACCAA GTTCGAGAAG 
AACTTCATCT CGCAGGGCAA CTATGAGAAC CGCACTGTCT TCGAATCCCT GGACATTGGC 
TGGCAGCTGC TGCGTATCTT CCCCAAGGAG ATGCTCAAGC GTATCCCGGC CTCCATCCTG 
GCCGAATTCT ACCCTAGGGA CTCGCGCCAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vha55 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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