Blm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Blm
Entrez Gene ID	41366
Full Name	Bloom syndrome helicase
Synonyms	BLM,CG6920,DmBLM,DmBlm,DmKu70,Dmblm,Dmel\CG6920,Ku70,MUS309,Mus309,RecQ,blm,dBLM,dmblm,ku70,mus 309,mus309
General protein information	Preferred Names Bloom syndrome helicase Names CG6920 gene product from transcript CG6920-RA Blm-PA Bloom's helicase CG6920-PA RecQ-like bloom bloom helicase bloom syndrome helicase bloom syndrome helicase ortholog drosophila Blm genome stabilizer 1 gesta1 mutagen-sensitive 309
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86E17-86E17

mRNA and Protein(s)

mRNA	Protein	Name
NM_079595.3	NP_524319.2	bloom syndrome helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dme-miR-314-3p modulation in Cr(VI) exposed Drosophila affects DNA damage repair by targeting mus309
Title: Dme-miR-314-3p modulation in Cr(VI) exposed Drosophila affects DNA damage repair by targeting mus309.

Drosophila BLM suppresses error-prone alternative end-joining repair of DNA double-strand breaks that can result in genome instability and tumor formation during aging.
Title: Loss of the bloom syndrome helicase increases DNA ligase 4-independent genome rearrangements and tumorigenesis in aging Drosophila.

these studies of synthetic lethality provide insights into the multiple functions of DmBLM and how various endonucleases may function when DmBLM is absent
Title: Three structure-selective endonucleases are essential in the absence of BLM helicase in Drosophila.

Data show that excision screens in mus309 mutant flies that lack DmBlm increases the percentage and overall size of flanking deletions recovered after excision of either P or Minos elements.
Title: Removal of the bloom syndrome DNA helicase extends the utility of imprecise transposon excision for making null mutations in Drosophila.

The suppression of homeologous single strand annealing depends on the Bloom helicase (Blm), encoded by the Drosophila gene mus309.
Title: Drosophila bloom helicase maintains genome integrity by inhibiting recombination between divergent DNA sequences.

The following Blm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Blm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04518	NM_079595.3 Latest version!	Drosophila melanogaster bloom syndrome helicase (Blm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Blm ( NM_079595.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524319.2 Drosophila melanogaster bloom syndrome helicase (Blm), mRNA.

Price & Availability↑

CloneID	OFa04518
Clone ID Related Accession (Same CDS sequence)	NM_079595.3
Accession Version	NM_079595.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bloom syndrome helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079595.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAGA AGCCTGTCGC GCAAAGAAAA CAGCTCACAC TTAGCAGCTT CATTGGCCTG 
GATGGCAATT CCCAGAGTCA ACCTAAGAGC AGAGCCGCAA GTGTCAGAAG CAAGCCCCCC 
GCAGTCTACA ATCCCATCTT TCTGGACGCG TCCTCATCCG ACGATGAAAC TACGGAGATA 
TCCAGTCAAT CCAACAATGG CACTATTGCT ACGAAGAAGT CCAGCAGAGA TCCTAGAACC 
GCAAAGCTCA AAAAACACAC ATACCTCGAT CTGTCAGTGT CCCCACTAGC TAAACTTTCT 
GCCAAGAAAT ATGCCAGAGA TTCGCCCAAA AAGCCCACAT CCCTCGATCT GTCAGTGTCC 
CCACTAGCTG AACTTCTTGC CAAAAAATCT GACAGAGATT CGCCCAAAAA GCCAGTACAG 
AATGAGAATA GTTACACCTA TCGGGGATTG TCGGAATCTC CTGTTGAAAA TAAATCAATT 
GGCGATACAC TAAGGAAACC ACCTCAAAAA GAGAGGAAAA CATCAATAGT TTGGCTTTCG 
GATTCTCCGG AAAAGAAAGT GACGCAAAAT GAGAGGAAGA TCCTAGACTC TCCACTCCAA 
AGGTTCTCCT TCGAGGACTT TCCAAACAAA GAAAATGGAA ATAGACATCA CCTACTCACA 
CTGCCTGATT CGCCGCCCCC TCCGCAGCCT GTCAAGAAAC CGGAGAAAAC TATGTGGCAA 
AATGAAACAA AAACGATTCA GGATAAGGAC TCCCCTGCAA ACCCATTAGT TAGCAACAAT 
CTGGCCTCCA TTTCGACTCT GCTTGACTCA TCGCGGGCTC CTAATACATA TAAAGGCAGT 
AGTCGTAATC TTTTTGAGGA TTCTCCAGAG AAAAGTGGCA GCGGGGAGCA AGGCTATAAA 
CTGGGTTCAG CGAAAGAAAA TGAAATCCCG ACAAAGCCTG CGACCGCTTC TCTGGAAAGA 
AATAGCGTCA CCTCCTCTCC ATCACCAGCG GCACCATTGA AGCCCAGATA CAGTGTGGCT 
TTTGACAATT CTCTGGCTGA TTACTTAAAG GACTTGGCCC AAAACGACAA CTTCAGCATC 
GATCCCAATA AACAGAATAC TGAGACACTG AAGAGCACGC TTGGATTCTT TCGCAACACT 
TATGTGGAGC TAATGGAAAA ATATTGCTCT CTGATTGACC AGATTCCCGC CATGCACTTC 
AATGAAATAG CAGGCTTTCA GCCGAATACG TTTCTCAAGC TCAAAGTAAT GCGACAAAAA 
TTTAAAGCGC GGACACAGCT TGTCCAAAAC AGTTTAGATA AGAAAGAAAG TCAGCTAAAA 
GCGGAACAGG AAGCCCTTGA GAAGGAGGAA ATAGAAATGC AGGCGGAACA GGCTCAGCAG 
ACTGTTTTAT CATCATCTTC ACCAGAGAAA AGTCGCCCGA TAATGCCCCT GCCAAAAGTT 
CAAGAAATCA AGGATGAAAA AATACCCAAT CGGAATCAGC TTATACACGA CCTTTGCGGA 
GAGCCAGACA ATTTCTCTCC ACCCAGCTCG CCGCGCGATA CGCAACTTAT TCCGAAACGC 
CAGCAGCTCA TCAACGACCT TTGCGGGGAG CCGGATGACT TCTCACCACC GAGCAAACAA 
AATGATCCGC ACCTGCTGCG AAAGTGCGAA GAACTTGTCC ATGACCTTTG CGAGGAACCG 
GACGACTACT TAGCACAGAG CATGATGCTA GACGGCGACT TGGAGGAGGA ACAACTGAAC 
GGACCCACGC AGGGGACGAC AACCAGCGGA ATGGATGATG ACGAAGATGA CTTGGAGGGA 
TTGCTGGCGG AAATCGAGGA CGAGCATCAG AAGATGCAGG GCCGCAGGTC AGAGTTCAAT 
GGTTACAGCT ATAAGGAGTT GGAAGCTGTC AAAGTAAAGG AGAAGCACAA GGAGACCCCG 
ATAAATATTT CCTTGGATGA TGATGGATTT CCCGAGTACG ATGAGGCTAT GTTTGAACAA 
ATGCATTCCC AGGCTGCTGC CAATAAAAGC AGAGTGAGCA GTGCCGGCCC AAGCACCAGT 
AAGAGCGTTG TGCCTACCAA GCAAACCAGT GCCCTGCACT CCCAAAAGCT GTCAGGGAAT 
TTCCATGCGA ACGTGCACAA TGACGGCATC ACCGGGGAGT TCGATGGTCA AAAGTTTGAG 
CACTCCACCC GCCTCATGCA CGGACTGAGC TACAGCTTTG GTCTAAAAAG TTTCCGTCCC 
AATCAGCTGC AGGTGATCAA CGCCACGCTT CTGGGCAATG ACTGCTTCGT CCTGATGCCA 
ACTGGTGGAG GAAAGTCGTT GTGCTATCAG CTGCCTGCCA TTCTGACCGA GGGAGTGACC 
ATTGTTATCA GTCCGCTGAA GTCGCTGATC TTTGATCAAA TCAATAAACT GGCTTCCCTA 
GATATCTGTG CAAAGAGTCT GAGCGGAGAA CAAAAAATGG CCGATGTCAT GGCCATCTAC 
AGGGATTTGG AGTCTCAGCC ACCTATGGTC AAATTGCTCT ACGTGACACC TGAGAAAATT 
AGCAGTTCTG CTCGCTTCCA GGACACTCTG GACACGCTTA ATTCCAACAA CTATATAAGT 
AGATTCGTTA TAGACGAGGC CCATTGCGTT TCCCAGTGGG GTCATGACTT TAGACCGGAC 
TACAAAAAGC TTGGAGTACT AAAGAAGCGT TTTCCAAATG TGCCTACTAT TGCGTTAACA 
GCTACAGCCA CGCCCCGCGT CCGCTTGGAT ATTCTGGCCC AACTAAATCT GAAGAACTGC 
AAGTGGTTCC TCTCCAGTTT TAATCGCAGC AACCTACGGT ACAGGGTACT GCCCAAAAAG 
GGCGTCTCGA CGTTGGACGA CATTAGCAGG TACATCCGCA GCAAACCGCA GCACTTCAGT 
GGCATAATCT ACTGCCTGTC GCGCAAGGAG TGCGATGAGA CGTCAAAGAA AATGTGCAAG 
GATGGCGTCC GGGCGGTATC CTATCATGCT GGTCTCACGG ACACTGACCG TGAGAGTCGG 
CAAAAGGATT GGCTGACCGG AAAGATGAGG GTAATCTGTG CCACCGTCGC CTTCGGCATG 
GGCATCGACA AGCCAGATGT CCGATTTGTT TTGCACTACT CACTGCCCAA GTCCATTGAG 
GGTTATTACC AAGAGGCAGG TCGCGCGGGC CGAGACGGTG ACGTGGCTGA TTGCATCTTG 
TACTACAACT ATTCGGACAT GCTCAGAATC AAGAAAATGC TGGACTCCGA CAAAGCTCTT 
CAGTACAATG TGAAGAAGAT ACATGTAGAC AATTTATATC GCATTGTTGG GTACTGTGAA 
AATCTCACAG ATTGCCGACG TGCCCAGCAG TTGGACTATT TTGGGGAGCA CTTTACCAGC 
GAACAATGTC TGGAGAATAG AGAGACAGCT TGCGATAATT GCATTAACAA ACGAGCCTAT 
AAGGCGGTAG ATGCATTGGA GCATGCTCGT AAGGCTGCAC GAGCAGTCAA GGATCTGTGC 
AGCGGTAGAT CCCGTTTCAC ACTATTGCAT ATTGCCGACG TACTGAAAGG TTCGAAGATC 
AAGAAAATCA TTGACTTTAA CCATCACAAA ACGCCACACC ATGGCGTCCT GAAAGACTGG 
GACAAGAACG ATGTACACAG GCTGCTGCGC AAAATGGTCA TCGATGGTTT CCTTAGGGAA 
GACCTTATAT TTACGAACGA TTTTCCACAG GCCTATTTGT ATCTGGGCAA CAACATCAGT 
AAACTAATGG AGGGCACGCC CAACTTCGAG TTTGCAGTGA CCAAAAATGC CAAGGAAGCC 
AAGGCGGCAG TTGGCAGCGT TTCCGATGGT GCAACGAGCA GTACCGCTGA TGGACAGTCG 
GGAATGCGGG AGATTCACGA GCGTTGCTAT ACAGATCTAT TGGATCTATG TCGAACCATT 
GCCAGCCAGC GAAACGTTAC CATGGCTAGT ATCATGAACA TTCAGGCCCT GAAGAGCATG 
GCCGAAACAC TACCAATCAC CGAGAAGGAC ATGTGCAGCA TTCCGCACGT GACCAAGGCG 
AACTTTGATA AATACGGCGC CAAGCTGCTC GAAATCACTA GCAACTATGC ATCGGAGAAG 
CTGCTGATGC AAGCCGTCCT GGACGAAGAG GAGGAACAGG CGGCCGCCAA GCAGAGGCCC 
AGTACATCCG GCTGGAATAA CGAAAGCGTG GACTGGGACA TGGCGGTTGC CTCACAGGGC 
AATGCAAACA CGAGCGGTGC CAGCGGTTTC AACTCGTTTA GAGCGGGCAA AAGAAAAAAG 
ATATACAAGT CAGGGGCCAG CAAGAGGTAC AAAACGAGTA CTACATCGCC AGCGGCCAGG 
AAGACAACAT CAGCCAGGGG TCGCGGTGGT AGGGCTGGCG CCAAACGAGC GGAGAGTTCA 
GCTTCGTCAG CGTCGGGCTG GAAGTCCAAG AAAACAGGGA ATAGCTTTGG CTTCGATTTA 
ATGCCACTGC CAGGATCAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524319.2
CDS	127..4590
Translation	MSKKPVAQRKQLTLSSFIGLDGNSQSQPKSRAASVRSKPPAVYNPIFLDASSSDDETTEISSQSNNGTIATKKSSRDPRTAKLKKHTYLDLSVSPLAKLSAKKYARDSPKKPTSLDLSVSPLAELLAKKSDRDSPKKPVQNENSYTYRGLSESPVENKSIGDTLRKPPQKERKTSIVWLSDSPEKKVTQNERKILDSPLQRFSFEDFPNKENGNRHHLLTLPDSPPPPQPVKKPEKTMWQNETKTIQDKDSPANPLVSNNLASISTLLDSSRAPNTYKGSSRNLFEDSPEKSGSGEQGYKLGSAKENEIPTKPATASLERNSVTSSPSPAAPLKPRYSVAFDNSLADYLKDLAQNDNFSIDPNKQNTETLKSTLGFFRNTYVELMEKYCSLIDQIPAMHFNEIAGFQPNTFLKLKVMRQKFKARTQLVQNSLDKKESQLKAEQEALEKEEIEMQAEQAQQTVLSSSSPEKSRPIMPLPKVQEIKDEKIPNRNQLIHDLCGEPDNFSPPSSPRDTQLIPKRQQLINDLCGEPDDFSPPSKQNDPHLLRKCEELVHDLCEEPDDYLAQSMMLDGDLEEEQLNGPTQGTTTSGMDDDEDDLEGLLAEIEDEHQKMQGRRSEFNGYSYKELEAVKVKEKHKETPINISLDDDGFPEYDEAMFEQMHSQAAANKSRVSSAGPSTSKSVVPTKQTSALHSQKLSGNFHANVHNDGITGEFDGQKFEHSTRLMHGLSYSFGLKSFRPNQLQVINATLLGNDCFVLMPTGGGKSLCYQLPAILTEGVTIVISPLKSLIFDQINKLASLDICAKSLSGEQKMADVMAIYRDLESQPPMVKLLYVTPEKISSSARFQDTLDTLNSNNYISRFVIDEAHCVSQWGHDFRPDYKKLGVLKKRFPNVPTIALTATATPRVRLDILAQLNLKNCKWFLSSFNRSNLRYRVLPKKGVSTLDDISRYIRSKPQHFSGIIYCLSRKECDETSKKMCKDGVRAVSYHAGLTDTDRESRQKDWLTGKMRVICATVAFGMGIDKPDVRFVLHYSLPKSIEGYYQEAGRAGRDGDVADCILYYNYSDMLRIKKMLDSDKALQYNVKKIHVDNLYRIVGYCENLTDCRRAQQLDYFGEHFTSEQCLENRETACDNCINKRAYKAVDALEHARKAARAVKDLCSGRSRFTLLHIADVLKGSKIKKIIDFNHHKTPHHGVLKDWDKNDVHRLLRKMVIDGFLREDLIFTNDFPQAYLYLGNNISKLMEGTPNFEFAVTKNAKEAKAAVGSVSDGATSSTADGQSGMREIHERCYTDLLDLCRTIASQRNVTMASIMNIQALKSMAETLPITEKDMCSIPHVTKANFDKYGAKLLEITSNYASEKLLMQAVLDEEEEQAAAKQRPSTSGWNNESVDWDMAVASQGNANTSGASGFNSFRAGKRKKIYKSGASKRYKTSTTSPAARKTTSARGRGGRAGAKRAESSASSASGWKSKKTGNSFGFDLMPLPGSK

Target ORF information:

RefSeq Version	NM_079595.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bloom syndrome helicase (Blm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079595.3

ORF Insert Sequence:

ATGTCCAAGA AGCCTGTCGC GCAAAGAAAA CAGCTCACAC TTAGCAGCTT CATTGGCCTG 
GATGGCAATT CCCAGAGTCA ACCTAAGAGC AGAGCCGCAA GTGTCAGAAG CAAGCCCCCC 
GCAGTCTACA ATCCCATCTT TCTGGACGCG TCCTCATCCG ACGATGAAAC TACGGAGATA 
TCCAGTCAAT CCAACAATGG CACTATTGCT ACGAAGAAGT CCAGCAGAGA TCCTAGAACC 
GCAAAGCTCA AAAAACACAC ATACCTCGAT CTGTCAGTGT CCCCACTAGC TAAACTTTCT 
GCCAAGAAAT ATGCCAGAGA TTCGCCCAAA AAGCCCACAT CCCTCGATCT GTCAGTGTCC 
CCACTAGCTG AACTTCTTGC CAAAAAATCT GACAGAGATT CGCCCAAAAA GCCAGTACAG 
AATGAGAATA GTTACACCTA TCGGGGATTG TCGGAATCTC CTGTTGAAAA TAAATCAATT 
GGCGATACAC TAAGGAAACC ACCTCAAAAA GAGAGGAAAA CATCAATAGT TTGGCTTTCG 
GATTCTCCGG AAAAGAAAGT GACGCAAAAT GAGAGGAAGA TCCTAGACTC TCCACTCCAA 
AGGTTCTCCT TCGAGGACTT TCCAAACAAA GAAAATGGAA ATAGACATCA CCTACTCACA 
CTGCCTGATT CGCCGCCCCC TCCGCAGCCT GTCAAGAAAC CGGAGAAAAC TATGTGGCAA 
AATGAAACAA AAACGATTCA GGATAAGGAC TCCCCTGCAA ACCCATTAGT TAGCAACAAT 
CTGGCCTCCA TTTCGACTCT GCTTGACTCA TCGCGGGCTC CTAATACATA TAAAGGCAGT 
AGTCGTAATC TTTTTGAGGA TTCTCCAGAG AAAAGTGGCA GCGGGGAGCA AGGCTATAAA 
CTGGGTTCAG CGAAAGAAAA TGAAATCCCG ACAAAGCCTG CGACCGCTTC TCTGGAAAGA 
AATAGCGTCA CCTCCTCTCC ATCACCAGCG GCACCATTGA AGCCCAGATA CAGTGTGGCT 
TTTGACAATT CTCTGGCTGA TTACTTAAAG GACTTGGCCC AAAACGACAA CTTCAGCATC 
GATCCCAATA AACAGAATAC TGAGACACTG AAGAGCACGC TTGGATTCTT TCGCAACACT 
TATGTGGAGC TAATGGAAAA ATATTGCTCT CTGATTGACC AGATTCCCGC CATGCACTTC 
AATGAAATAG CAGGCTTTCA GCCGAATACG TTTCTCAAGC TCAAAGTAAT GCGACAAAAA 
TTTAAAGCGC GGACACAGCT TGTCCAAAAC AGTTTAGATA AGAAAGAAAG TCAGCTAAAA 
GCGGAACAGG AAGCCCTTGA GAAGGAGGAA ATAGAAATGC AGGCGGAACA GGCTCAGCAG 
ACTGTTTTAT CATCATCTTC ACCAGAGAAA AGTCGCCCGA TAATGCCCCT GCCAAAAGTT 
CAAGAAATCA AGGATGAAAA AATACCCAAT CGGAATCAGC TTATACACGA CCTTTGCGGA 
GAGCCAGACA ATTTCTCTCC ACCCAGCTCG CCGCGCGATA CGCAACTTAT TCCGAAACGC 
CAGCAGCTCA TCAACGACCT TTGCGGGGAG CCGGATGACT TCTCACCACC GAGCAAACAA 
AATGATCCGC ACCTGCTGCG AAAGTGCGAA GAACTTGTCC ATGACCTTTG CGAGGAACCG 
GACGACTACT TAGCACAGAG CATGATGCTA GACGGCGACT TGGAGGAGGA ACAACTGAAC 
GGACCCACGC AGGGGACGAC AACCAGCGGA ATGGATGATG ACGAAGATGA CTTGGAGGGA 
TTGCTGGCGG AAATCGAGGA CGAGCATCAG AAGATGCAGG GCCGCAGGTC AGAGTTCAAT 
GGTTACAGCT ATAAGGAGTT GGAAGCTGTC AAAGTAAAGG AGAAGCACAA GGAGACCCCG 
ATAAATATTT CCTTGGATGA TGATGGATTT CCCGAGTACG ATGAGGCTAT GTTTGAACAA 
ATGCATTCCC AGGCTGCTGC CAATAAAAGC AGAGTGAGCA GTGCCGGCCC AAGCACCAGT 
AAGAGCGTTG TGCCTACCAA GCAAACCAGT GCCCTGCACT CCCAAAAGCT GTCAGGGAAT 
TTCCATGCGA ACGTGCACAA TGACGGCATC ACCGGGGAGT TCGATGGTCA AAAGTTTGAG 
CACTCCACCC GCCTCATGCA CGGACTGAGC TACAGCTTTG GTCTAAAAAG TTTCCGTCCC 
AATCAGCTGC AGGTGATCAA CGCCACGCTT CTGGGCAATG ACTGCTTCGT CCTGATGCCA 
ACTGGTGGAG GAAAGTCGTT GTGCTATCAG CTGCCTGCCA TTCTGACCGA GGGAGTGACC 
ATTGTTATCA GTCCGCTGAA GTCGCTGATC TTTGATCAAA TCAATAAACT GGCTTCCCTA 
GATATCTGTG CAAAGAGTCT GAGCGGAGAA CAAAAAATGG CCGATGTCAT GGCCATCTAC 
AGGGATTTGG AGTCTCAGCC ACCTATGGTC AAATTGCTCT ACGTGACACC TGAGAAAATT 
AGCAGTTCTG CTCGCTTCCA GGACACTCTG GACACGCTTA ATTCCAACAA CTATATAAGT 
AGATTCGTTA TAGACGAGGC CCATTGCGTT TCCCAGTGGG GTCATGACTT TAGACCGGAC 
TACAAAAAGC TTGGAGTACT AAAGAAGCGT TTTCCAAATG TGCCTACTAT TGCGTTAACA 
GCTACAGCCA CGCCCCGCGT CCGCTTGGAT ATTCTGGCCC AACTAAATCT GAAGAACTGC 
AAGTGGTTCC TCTCCAGTTT TAATCGCAGC AACCTACGGT ACAGGGTACT GCCCAAAAAG 
GGCGTCTCGA CGTTGGACGA CATTAGCAGG TACATCCGCA GCAAACCGCA GCACTTCAGT 
GGCATAATCT ACTGCCTGTC GCGCAAGGAG TGCGATGAGA CGTCAAAGAA AATGTGCAAG 
GATGGCGTCC GGGCGGTATC CTATCATGCT GGTCTCACGG ACACTGACCG TGAGAGTCGG 
CAAAAGGATT GGCTGACCGG AAAGATGAGG GTAATCTGTG CCACCGTCGC CTTCGGCATG 
GGCATCGACA AGCCAGATGT CCGATTTGTT TTGCACTACT CACTGCCCAA GTCCATTGAG 
GGTTATTACC AAGAGGCAGG TCGCGCGGGC CGAGACGGTG ACGTGGCTGA TTGCATCTTG 
TACTACAACT ATTCGGACAT GCTCAGAATC AAGAAAATGC TGGACTCCGA CAAAGCTCTT 
CAGTACAATG TGAAGAAGAT ACATGTAGAC AATTTATATC GCATTGTTGG GTACTGTGAA 
AATCTCACAG ATTGCCGACG TGCCCAGCAG TTGGACTATT TTGGGGAGCA CTTTACCAGC 
GAACAATGTC TGGAGAATAG AGAGACAGCT TGCGATAATT GCATTAACAA ACGAGCCTAT 
AAGGCGGTAG ATGCATTGGA GCATGCTCGT AAGGCTGCAC GAGCAGTCAA GGATCTGTGC 
AGCGGTAGAT CCCGTTTCAC ACTATTGCAT ATTGCCGACG TACTGAAAGG TTCGAAGATC 
AAGAAAATCA TTGACTTTAA CCATCACAAA ACGCCACACC ATGGCGTCCT GAAAGACTGG 
GACAAGAACG ATGTACACAG GCTGCTGCGC AAAATGGTCA TCGATGGTTT CCTTAGGGAA 
GACCTTATAT TTACGAACGA TTTTCCACAG GCCTATTTGT ATCTGGGCAA CAACATCAGT 
AAACTAATGG AGGGCACGCC CAACTTCGAG TTTGCAGTGA CCAAAAATGC CAAGGAAGCC 
AAGGCGGCAG TTGGCAGCGT TTCCGATGGT GCAACGAGCA GTACCGCTGA TGGACAGTCG 
GGAATGCGGG AGATTCACGA GCGTTGCTAT ACAGATCTAT TGGATCTATG TCGAACCATT 
GCCAGCCAGC GAAACGTTAC CATGGCTAGT ATCATGAACA TTCAGGCCCT GAAGAGCATG 
GCCGAAACAC TACCAATCAC CGAGAAGGAC ATGTGCAGCA TTCCGCACGT GACCAAGGCG 
AACTTTGATA AATACGGCGC CAAGCTGCTC GAAATCACTA GCAACTATGC ATCGGAGAAG 
CTGCTGATGC AAGCCGTCCT GGACGAAGAG GAGGAACAGG CGGCCGCCAA GCAGAGGCCC 
AGTACATCCG GCTGGAATAA CGAAAGCGTG GACTGGGACA TGGCGGTTGC CTCACAGGGC 
AATGCAAACA CGAGCGGTGC CAGCGGTTTC AACTCGTTTA GAGCGGGCAA AAGAAAAAAG 
ATATACAAGT CAGGGGCCAG CAAGAGGTAC AAAACGAGTA CTACATCGCC AGCGGCCAGG 
AAGACAACAT CAGCCAGGGG TCGCGGTGGT AGGGCTGGCG CCAAACGAGC GGAGAGTTCA 
GCTTCGTCAG CGTCGGGCTG GAAGTCCAAG AAAACAGGGA ATAGCTTTGG CTTCGATTTA 
ATGCCACTGC CAGGATCAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Blm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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