CG6621 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG6621
Entrez Gene ID	41319
Full Name	CG6621 gene product from transcript CG6621-RB
Synonyms	Dmel\CG6621,TTC14
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86C7-86C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001202281.2	NP_001189210.1	uncharacterized protein, isoform B
NM_141779.3	NP_650036.2	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG6621 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG6621 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04479	NM_001202281.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG6621), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08324	NM_141779.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG6621), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG6621 ( NM_001202281.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189210.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG6621), mRNA.

Price & Availability↑

CloneID	OFa04479
Clone ID Related Accession (Same CDS sequence)	NM_001202281.2
Accession Version	NM_001202281.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001202281.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTTCG ATTACATCGG CCAGGCCCTG GGCTACCACG GGCAGCCGCT CCAGAAGATC 
TGGGACGACG AGCGCGGCGT GGACGACCTG CGGCTGATGG GACTCACCCA GGTGAACTAC 
GGGGTCTACC AGGAGCGGCA GAAGTACTTC ACCTTCCAGG AGCGGGCTAA GCGATTGAAG 
ATGCACCAGT TTCTGGCCAG GAAGGCCACC GATTTGTATG ATCGCACGCT GGTGGCCAAT 
GTTATGGAGG ATTCCCTGCT GGCACAAGGC AACACATACA TGACTCAGAT GGCCCCCTTC 
GAGTTCTTCC TCAATGTGAA GGACAAACGC AAGGGATGGG CGCACCGATT GTCTGCACTG 
AAGCAGGGCG ACATCATCTA CACGCAGGTG ACACGGCTGG CCAGCGGCAA CCGGCTCATA 
GTGAAGCCAC TCTGCACTGC TGAGCCCAAA CACGCCTACT TGGCTGACAT TCCCATAAAG 
GCCGTGATCC TGCAGGACTT CTGGGGTCCC TTGCCCCTGG ACAAGCAGGG CAATCCGCGC 
AGCTTTGTCC AGAATGACAT CTTGCGCTGT GAGATAAACA ACATATCCGC GGATACCGAG 
CGGCTCTCTC TGAACATGAT CGGCATGTTC AATAAGGTGC CGGATTTAAA GTTTGGTCTC 
TGCGACTTTA AGGAGTTGCC CAAGTATTAT AGTAAAATAC ATAACCTGGA TCATCCATCT 
GCGTCTCACT ACGAGGATGA GCTAAACAAA GCCTTAGAGT TTGAAAATCC CAACTACGAT 
TTGCTGTTCC AAATGAACGG ACTGCAGCCA AACGAGAATC TGTCACTGAT GTCATATTTA 
AGGGCGGGAT TCCCGGAGGA GGAGAACGCC GCGGAGTTGC GACAGAAGCA GGCCTCCCAG 
TGGGCCTTCC GCTCCGTGGC TGATGGCATC GAACACTTTA AGAACGGCCA ACAGGTCGAG 
GCCTTTCAGT GCCTGAACAA GGCCTTAAAC ATTGATCCGC GCAATGTTGA GGCGCTGGTG 
GCCCGCGGTG CCCTCTACGC GAATCGCGGC AGCTTTCTTA AAGGGCTGCA GGACTTTGAG 
AAGGCTCTGC ACCTGAACAA GTATCACGTG AACGCGCGCA AATACATGGG CGAAACGCTG 
GTGGCTCTGG GTCGCAGCTA CGAAGAGGAG AATCGCATAG CCGAAGCCGT CAAGGCCTAT 
AGCGATTGCC TAAATCTGTT GCCGCTGCAC GAAGAAGCAC GCCAGTCCCT GGACGCACTG 
CATCGAAGTG GAGACGGTAA GCGTAGAATA AGCACTGAGC CGTCGAACAT GACCGGTGGC 
CAGTCCTCGG ACGAGTCCTC CTCATCGAGT GCCAGTGATA GTGAATGCGA AGATTCGGCT 
GGTAAGCCCA AGTCAAACAT TAGTCAGCCC TTTTATGCCC AACACAAGCT AAAGCAATCG 
GGAGATGAAA AGTTGGCCGT CATTGATGCC CATAAGGCCA ATGAGTTCCG TTTGGACGAC 
GACGAGACCG TGTCGAGTGT GCGGAAGCTT TTGCGCGAGG CGAGCAAACA CAAGAAGGCC 
AAGAAGAAGG GAAAGAAGAA AAAGGACAAG AAGGAGCGAA GGAGGTCCAG GACCAAGTCC 
GAAACGGAGG ATCCCATTGA ATTGCTTAAG AAGATCGACT TCCAGGAGGC CCTCAGGCTC 
ATGAACAGCA CCAGGAGCGA TGAGGAACGC AAGGCCAAGA TCAAGAGCTA TCTAAAAGAA 
GGAGGAAAAG AGTCACCACC TCCCCCGCCA CCAATGAAAA AAACAGCAGT CTCATCTAGA 
AAGTCAAGCT TTGACAATGT GGGACCCAGC ACATCGCGTC ATGCGGCCTC CGTTGTTGGC 
TTTGGAGGCG CCCAGCCACC ACCAGCGCCC AAGTTTCTGG GGCAAAAGAA ACATCCGGAG 
CCGGCACCCA AGCTCTCCTT TCAGATTAAG AAACGACCGC TGCAGATGGA CAAGCTGGGA 
ATGCTGCGTC TAGCAGCGCC AGCGGAGCCA CTCAAGGGAG GACGGAGCAG CAGCTCCCGC 
AGCCGGAGTA GGAGTAGAAG CCGCTCGCGA AGTCGCTCAC GTAATCGTCG GCTTGGAAGG 
AGCACGCGTT CGTCGCTCAG GAGCCGGCGT TCTGGCTCGA GGTCCAGATC GATTAGCCGA 
TCACGGTCGC GTCGCCGACG TACCTACTCC CGTTCGCGCA GCCCCTCGCC ACGCAGTTAC 
GGAAATCGCT TTGTGATTGG ACGCTACCGT AATCGCCGAA CTCGGCGTTC GCGCTCCTTT 
CATCGTCGGC GTTCTCCATC GCCCATTTTG AAACGCCGAT CGCCTTCACC ATTTGTGCCC 
CGTCGCACAC CATCGCCGGC ATCCCGCTAT CGACGCAGCC GATCCCGCAG TCGCTCCCGC 
TCACGCAGTC GGAGTCCTCG ACGCTTTCAG TCTCGCTACA TGCCGAGGGA TCGTGGCTCC 
GTGAATCGAA GCAGGTACTC CTGGCACAAT CCCCATAAAC GCAGTGTATC ACGCACCGTG 
GAGCCAAATG GCAAAGATGA AAGGGCAGCT ACTCCGCCGA AAAAGGGTGT TGCTGATGAT 
AGATCTGGAC GAGAAGAAAG GGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189210.1
CDS	350..2956
Translation	MNFDYIGQALGYHGQPLQKIWDDERGVDDLRLMGLTQVNYGVYQERQKYFTFQERAKRLKMHQFLARKATDLYDRTLVANVMEDSLLAQGNTYMTQMAPFEFFLNVKDKRKGWAHRLSALKQGDIIYTQVTRLASGNRLIVKPLCTAEPKHAYLADIPIKAVILQDFWGPLPLDKQGNPRSFVQNDILRCEINNISADTERLSLNMIGMFNKVPDLKFGLCDFKELPKYYSKIHNLDHPSASHYEDELNKALEFENPNYDLLFQMNGLQPNENLSLMSYLRAGFPEEENAAELRQKQASQWAFRSVADGIEHFKNGQQVEAFQCLNKALNIDPRNVEALVARGALYANRGSFLKGLQDFEKALHLNKYHVNARKYMGETLVALGRSYEEENRIAEAVKAYSDCLNLLPLHEEARQSLDALHRSGDGKRRISTEPSNMTGGQSSDESSSSSASDSECEDSAGKPKSNISQPFYAQHKLKQSGDEKLAVIDAHKANEFRLDDDETVSSVRKLLREASKHKKAKKKGKKKKDKKERRRSRTKSETEDPIELLKKIDFQEALRLMNSTRSDEERKAKIKSYLKEGGKESPPPPPPMKKTAVSSRKSSFDNVGPSTSRHAASVVGFGGAQPPPAPKFLGQKKHPEPAPKLSFQIKKRPLQMDKLGMLRLAAPAEPLKGGRSSSSRSRSRSRSRSRSRSRNRRLGRSTRSSLRSRRSGSRSRSISRSRSRRRRTYSRSRSPSPRSYGNRFVIGRYRNRRTRRSRSFHRRRSPSPILKRRSPSPFVPRRTPSPASRYRRSRSRSRSRSRSRSPRRFQSRYMPRDRGSVNRSRYSWHNPHKRSVSRTVEPNGKDERAATPPKKGVADDRSGREERA

Target ORF information:

RefSeq Version	NM_001202281.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG6621), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202281.2

ORF Insert Sequence:

ATGAACTTCG ATTACATCGG CCAGGCCCTG GGCTACCACG GGCAGCCGCT CCAGAAGATC 
TGGGACGACG AGCGCGGCGT GGACGACCTG CGGCTGATGG GACTCACCCA GGTGAACTAC 
GGGGTCTACC AGGAGCGGCA GAAGTACTTC ACCTTCCAGG AGCGGGCTAA GCGATTGAAG 
ATGCACCAGT TTCTGGCCAG GAAGGCCACC GATTTGTATG ATCGCACGCT GGTGGCCAAT 
GTTATGGAGG ATTCCCTGCT GGCACAAGGC AACACATACA TGACTCAGAT GGCCCCCTTC 
GAGTTCTTCC TCAATGTGAA GGACAAACGC AAGGGATGGG CGCACCGATT GTCTGCACTG 
AAGCAGGGCG ACATCATCTA CACGCAGGTG ACACGGCTGG CCAGCGGCAA CCGGCTCATA 
GTGAAGCCAC TCTGCACTGC TGAGCCCAAA CACGCCTACT TGGCTGACAT TCCCATAAAG 
GCCGTGATCC TGCAGGACTT CTGGGGTCCC TTGCCCCTGG ACAAGCAGGG CAATCCGCGC 
AGCTTTGTCC AGAATGACAT CTTGCGCTGT GAGATAAACA ACATATCCGC GGATACCGAG 
CGGCTCTCTC TGAACATGAT CGGCATGTTC AATAAGGTGC CGGATTTAAA GTTTGGTCTC 
TGCGACTTTA AGGAGTTGCC CAAGTATTAT AGTAAAATAC ATAACCTGGA TCATCCATCT 
GCGTCTCACT ACGAGGATGA GCTAAACAAA GCCTTAGAGT TTGAAAATCC CAACTACGAT 
TTGCTGTTCC AAATGAACGG ACTGCAGCCA AACGAGAATC TGTCACTGAT GTCATATTTA 
AGGGCGGGAT TCCCGGAGGA GGAGAACGCC GCGGAGTTGC GACAGAAGCA GGCCTCCCAG 
TGGGCCTTCC GCTCCGTGGC TGATGGCATC GAACACTTTA AGAACGGCCA ACAGGTCGAG 
GCCTTTCAGT GCCTGAACAA GGCCTTAAAC ATTGATCCGC GCAATGTTGA GGCGCTGGTG 
GCCCGCGGTG CCCTCTACGC GAATCGCGGC AGCTTTCTTA AAGGGCTGCA GGACTTTGAG 
AAGGCTCTGC ACCTGAACAA GTATCACGTG AACGCGCGCA AATACATGGG CGAAACGCTG 
GTGGCTCTGG GTCGCAGCTA CGAAGAGGAG AATCGCATAG CCGAAGCCGT CAAGGCCTAT 
AGCGATTGCC TAAATCTGTT GCCGCTGCAC GAAGAAGCAC GCCAGTCCCT GGACGCACTG 
CATCGAAGTG GAGACGGTAA GCGTAGAATA AGCACTGAGC CGTCGAACAT GACCGGTGGC 
CAGTCCTCGG ACGAGTCCTC CTCATCGAGT GCCAGTGATA GTGAATGCGA AGATTCGGCT 
GGTAAGCCCA AGTCAAACAT TAGTCAGCCC TTTTATGCCC AACACAAGCT AAAGCAATCG 
GGAGATGAAA AGTTGGCCGT CATTGATGCC CATAAGGCCA ATGAGTTCCG TTTGGACGAC 
GACGAGACCG TGTCGAGTGT GCGGAAGCTT TTGCGCGAGG CGAGCAAACA CAAGAAGGCC 
AAGAAGAAGG GAAAGAAGAA AAAGGACAAG AAGGAGCGAA GGAGGTCCAG GACCAAGTCC 
GAAACGGAGG ATCCCATTGA ATTGCTTAAG AAGATCGACT TCCAGGAGGC CCTCAGGCTC 
ATGAACAGCA CCAGGAGCGA TGAGGAACGC AAGGCCAAGA TCAAGAGCTA TCTAAAAGAA 
GGAGGAAAAG AGTCACCACC TCCCCCGCCA CCAATGAAAA AAACAGCAGT CTCATCTAGA 
AAGTCAAGCT TTGACAATGT GGGACCCAGC ACATCGCGTC ATGCGGCCTC CGTTGTTGGC 
TTTGGAGGCG CCCAGCCACC ACCAGCGCCC AAGTTTCTGG GGCAAAAGAA ACATCCGGAG 
CCGGCACCCA AGCTCTCCTT TCAGATTAAG AAACGACCGC TGCAGATGGA CAAGCTGGGA 
ATGCTGCGTC TAGCAGCGCC AGCGGAGCCA CTCAAGGGAG GACGGAGCAG CAGCTCCCGC 
AGCCGGAGTA GGAGTAGAAG CCGCTCGCGA AGTCGCTCAC GTAATCGTCG GCTTGGAAGG 
AGCACGCGTT CGTCGCTCAG GAGCCGGCGT TCTGGCTCGA GGTCCAGATC GATTAGCCGA 
TCACGGTCGC GTCGCCGACG TACCTACTCC CGTTCGCGCA GCCCCTCGCC ACGCAGTTAC 
GGAAATCGCT TTGTGATTGG ACGCTACCGT AATCGCCGAA CTCGGCGTTC GCGCTCCTTT 
CATCGTCGGC GTTCTCCATC GCCCATTTTG AAACGCCGAT CGCCTTCACC ATTTGTGCCC 
CGTCGCACAC CATCGCCGGC ATCCCGCTAT CGACGCAGCC GATCCCGCAG TCGCTCCCGC 
TCACGCAGTC GGAGTCCTCG ACGCTTTCAG TCTCGCTACA TGCCGAGGGA TCGTGGCTCC 
GTGAATCGAA GCAGGTACTC CTGGCACAAT CCCCATAAAC GCAGTGTATC ACGCACCGTG 
GAGCCAAATG GCAAAGATGA AAGGGCAGCT ACTCCGCCGA AAAAGGGTGT TGCTGATGAT 
AGATCTGGAC GAGAAGAAAG GGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG6621 ( NM_141779.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650036.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG6621), mRNA.

Price & Availability↑

CloneID	OFa08324
Clone ID Related Accession (Same CDS sequence)	NM_141779.3
Accession Version	NM_141779.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141779.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTTCG ATTACATCGG CCAGGCCCTG GGCTACCACG GGCAGCCGCT CCAGAAGATC 
TGGGACGACG AGCGCGGCGT GGACGACCTG CGGCTGATGG GACTCACCCA GGTGAACTAC 
GGGGTCTACC AGGAGCGGCA GAAGTACTTC ACCTTCCAGG AGCGGGCTAA GCGATTGAAG 
ATGCACCAGT TTCTGGCCAG GAAGGCCACC GATTTGTATG ATCGCACGCT GGTGGCCAAT 
GTTATGGAGG ATTCCCTGCT GGCACAAGGC AACACATACA TGACTCAGAT GGCCCCCTTC 
GAGTTCTTCC TCAATGTGAA GGACAAACGC AAGGGATGGG CGCACCGATT GTCTGCACTG 
AAGCAGGGCG ACATCATCTA CACGCAGGTG ACACGGCTGG CCAGCGGCAA CCGGCTCATA 
GTGAAGCCAC TCTGCACTGC TGAGCCCAAA CACGCCTACT TGGCTGACAT TCCCATAAAG 
GCCGTGATCC TGCAGGACTT CTGGGGTCCC TTGCCCCTGG ACAAGCAGGG CAATCCGCGC 
AGCTTTGTCC AGAATGACAT CTTGCGCTGT GAGATAAACA ACATATCCGC GGATACCGAG 
CGGCTCTCTC TGAACATGAT CGGCATGTTC AATAAGGTGC CGGATTTAAA GTTTGGTCTC 
TGCGACTTTA AGGAGTTGCC CAAGTATTAT AGTAAAATAC ATAACCTGGA TCATCCATCT 
GCGTCTCACT ACGAGGATGA GCTAAACAAA GCCTTAGAGT TTGAAAATCC CAACTACGAT 
TTGCTGTTCC AAATGAACGG ACTGCAGCCA AACGAGAATC TGTCACTGAT GTCATATTTA 
AGGGCGGGAT TCCCGGAGGA GGAGAACGCC GCGGAGTTGC GACAGAAGCA GGCCTCCCAG 
TGGGCCTTCC GCTCCGTGGC TGATGGCATC GAACACTTTA AGAACGGCCA ACAGGTCGAG 
GCCTTTCAGT GCCTGAACAA GGCCTTAAAC ATTGATCCGC GCAATGTTGA GGCGCTGGTG 
GCCCGCGGTG CCCTCTACGC GAATCGCGGC AGCTTTCTTA AAGGGCTGCA GGACTTTGAG 
AAGGCTCTGC ACCTGAACAA GTATCACGTG AACGCGCGCA AATACATGGG CGAAACGCTG 
GTGGCTCTGG GTCGCAGCTA CGAAGAGGAG AATCGCATAG CCGAAGCCGT CAAGGCCTAT 
AGCGATTGCC TAAATCTGTT GCCGCTGCAC GAAGAAGCAC GCCAGTCCCT GGACGCACTG 
CATCGAAGTG GAGACGGTAA GCGTAGAATA AGCACTGAGC CGTCGAACAT GACCGGTGGC 
CAGTCCTCGG ACGAGTCCTC CTCATCGAGT GCCAGTGATA GTGAATGCGA AGATTCGGCT 
GGTAAGCCCA AGTCAAACAT TAGTCAGCCC TTTTATGCCC AACACAAGCT AAAGCAATCG 
GGAGATGAAA AGTTGGCCGT CATTGATGCC CATAAGGCCA ATGAGTTCCG TTTGGACGAC 
GACGAGACCG TGTCGAGTGT GCGGAAGCTT TTGCGCGAGG CGAGCAAACA CAAGAAGGCC 
AAGAAGAAGG GAAAGAAGAA AAAGGACAAG AAGGAGCGAA GGAGGTCCAG GACCAAGTCC 
GAAACGGAGG ATCCCATTGA ATTGCTTAAG AAGATCGACT TCCAGGAGGC CCTCAGACAA 
GACAACAAGC TCATGAACAG CACCAGGAGC GATGAGGAAC GCAAGGCCAA GATCAAGAGC 
TATCTAAAAG AAGGAGGAAA AGAGTCACCA CCTCCCCCGC CACCAATGAA AAAAACAGCA 
GTCTCATCTA GAAAGTCAAG CTTTGACAAT GTGGGACCCA GCACATCGCG TCATGCGGCC 
TCCGTTGTTG GCTTTGGAGG CGCCCAGCCA CCACCAGCGC CCAAGTTTCT GGGGCAAAAG 
AAACATCCGG AGCCGGCACC CAAGCTCTCC TTTCAGATTA AGAAACGACC GCTGCAGATG 
GACAAGCTGG GAATGCTGCG TCTAGCAGCG CCAGCGGAGC CACTCAAGGG AGGACGGAGC 
AGCAGCTCCC GCAGCCGGAG TAGGAGTAGA AGCCGCTCGC GAAGTCGCTC ACGTAATCGT 
CGGCTTGGAA GGAGCACGCG TTCGTCGCTC AGGAGCCGGC GTTCTGGCTC GAGGTCCAGA 
TCGATTAGCC GATCACGGTC GCGTCGCCGA CGTACCTACT CCCGTTCGCG CAGCCCCTCG 
CCACGCAGTT ACGGAAATCG CTTTGTGATT GGACGCTACC GTAATCGCCG AACTCGGCGT 
TCGCGCTCCT TTCATCGTCG GCGTTCTCCA TCGCCCATTT TGAAACGCCG ATCGCCTTCA 
CCATTTGTGC CCCGTCGCAC ACCATCGCCG GCATCCCGCT ATCGACGCAG CCGATCCCGC 
AGTCGCTCCC GCTCACGCAG TCGGAGTCCT CGACGCTTTC AGTCTCGCTA CATGCCGAGG 
GATCGTGGCT CCGTGAATCG AAGCAGGTAC TCCTGGCACA ATCCCCATAA ACGCAGTGTA 
TCACGCACCG TGGAGCCAAA TGGCAAAGAT GAAAGGGCAG CTACTCCGCC GAAAAAGGGT 
GTTGCTGATG ATAGATCTGG ACGAGAAGAA AGGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650036.2
CDS	350..2968
Translation	MNFDYIGQALGYHGQPLQKIWDDERGVDDLRLMGLTQVNYGVYQERQKYFTFQERAKRLKMHQFLARKATDLYDRTLVANVMEDSLLAQGNTYMTQMAPFEFFLNVKDKRKGWAHRLSALKQGDIIYTQVTRLASGNRLIVKPLCTAEPKHAYLADIPIKAVILQDFWGPLPLDKQGNPRSFVQNDILRCEINNISADTERLSLNMIGMFNKVPDLKFGLCDFKELPKYYSKIHNLDHPSASHYEDELNKALEFENPNYDLLFQMNGLQPNENLSLMSYLRAGFPEEENAAELRQKQASQWAFRSVADGIEHFKNGQQVEAFQCLNKALNIDPRNVEALVARGALYANRGSFLKGLQDFEKALHLNKYHVNARKYMGETLVALGRSYEEENRIAEAVKAYSDCLNLLPLHEEARQSLDALHRSGDGKRRISTEPSNMTGGQSSDESSSSSASDSECEDSAGKPKSNISQPFYAQHKLKQSGDEKLAVIDAHKANEFRLDDDETVSSVRKLLREASKHKKAKKKGKKKKDKKERRRSRTKSETEDPIELLKKIDFQEALRQDNKLMNSTRSDEERKAKIKSYLKEGGKESPPPPPPMKKTAVSSRKSSFDNVGPSTSRHAASVVGFGGAQPPPAPKFLGQKKHPEPAPKLSFQIKKRPLQMDKLGMLRLAAPAEPLKGGRSSSSRSRSRSRSRSRSRSRNRRLGRSTRSSLRSRRSGSRSRSISRSRSRRRRTYSRSRSPSPRSYGNRFVIGRYRNRRTRRSRSFHRRRSPSPILKRRSPSPFVPRRTPSPASRYRRSRSRSRSRSRSRSPRRFQSRYMPRDRGSVNRSRYSWHNPHKRSVSRTVEPNGKDERAATPPKKGVADDRSGREERA

Target ORF information:

RefSeq Version	NM_141779.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG6621), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141779.3

ORF Insert Sequence:

ATGAACTTCG ATTACATCGG CCAGGCCCTG GGCTACCACG GGCAGCCGCT CCAGAAGATC 
TGGGACGACG AGCGCGGCGT GGACGACCTG CGGCTGATGG GACTCACCCA GGTGAACTAC 
GGGGTCTACC AGGAGCGGCA GAAGTACTTC ACCTTCCAGG AGCGGGCTAA GCGATTGAAG 
ATGCACCAGT TTCTGGCCAG GAAGGCCACC GATTTGTATG ATCGCACGCT GGTGGCCAAT 
GTTATGGAGG ATTCCCTGCT GGCACAAGGC AACACATACA TGACTCAGAT GGCCCCCTTC 
GAGTTCTTCC TCAATGTGAA GGACAAACGC AAGGGATGGG CGCACCGATT GTCTGCACTG 
AAGCAGGGCG ACATCATCTA CACGCAGGTG ACACGGCTGG CCAGCGGCAA CCGGCTCATA 
GTGAAGCCAC TCTGCACTGC TGAGCCCAAA CACGCCTACT TGGCTGACAT TCCCATAAAG 
GCCGTGATCC TGCAGGACTT CTGGGGTCCC TTGCCCCTGG ACAAGCAGGG CAATCCGCGC 
AGCTTTGTCC AGAATGACAT CTTGCGCTGT GAGATAAACA ACATATCCGC GGATACCGAG 
CGGCTCTCTC TGAACATGAT CGGCATGTTC AATAAGGTGC CGGATTTAAA GTTTGGTCTC 
TGCGACTTTA AGGAGTTGCC CAAGTATTAT AGTAAAATAC ATAACCTGGA TCATCCATCT 
GCGTCTCACT ACGAGGATGA GCTAAACAAA GCCTTAGAGT TTGAAAATCC CAACTACGAT 
TTGCTGTTCC AAATGAACGG ACTGCAGCCA AACGAGAATC TGTCACTGAT GTCATATTTA 
AGGGCGGGAT TCCCGGAGGA GGAGAACGCC GCGGAGTTGC GACAGAAGCA GGCCTCCCAG 
TGGGCCTTCC GCTCCGTGGC TGATGGCATC GAACACTTTA AGAACGGCCA ACAGGTCGAG 
GCCTTTCAGT GCCTGAACAA GGCCTTAAAC ATTGATCCGC GCAATGTTGA GGCGCTGGTG 
GCCCGCGGTG CCCTCTACGC GAATCGCGGC AGCTTTCTTA AAGGGCTGCA GGACTTTGAG 
AAGGCTCTGC ACCTGAACAA GTATCACGTG AACGCGCGCA AATACATGGG CGAAACGCTG 
GTGGCTCTGG GTCGCAGCTA CGAAGAGGAG AATCGCATAG CCGAAGCCGT CAAGGCCTAT 
AGCGATTGCC TAAATCTGTT GCCGCTGCAC GAAGAAGCAC GCCAGTCCCT GGACGCACTG 
CATCGAAGTG GAGACGGTAA GCGTAGAATA AGCACTGAGC CGTCGAACAT GACCGGTGGC 
CAGTCCTCGG ACGAGTCCTC CTCATCGAGT GCCAGTGATA GTGAATGCGA AGATTCGGCT 
GGTAAGCCCA AGTCAAACAT TAGTCAGCCC TTTTATGCCC AACACAAGCT AAAGCAATCG 
GGAGATGAAA AGTTGGCCGT CATTGATGCC CATAAGGCCA ATGAGTTCCG TTTGGACGAC 
GACGAGACCG TGTCGAGTGT GCGGAAGCTT TTGCGCGAGG CGAGCAAACA CAAGAAGGCC 
AAGAAGAAGG GAAAGAAGAA AAAGGACAAG AAGGAGCGAA GGAGGTCCAG GACCAAGTCC 
GAAACGGAGG ATCCCATTGA ATTGCTTAAG AAGATCGACT TCCAGGAGGC CCTCAGACAA 
GACAACAAGC TCATGAACAG CACCAGGAGC GATGAGGAAC GCAAGGCCAA GATCAAGAGC 
TATCTAAAAG AAGGAGGAAA AGAGTCACCA CCTCCCCCGC CACCAATGAA AAAAACAGCA 
GTCTCATCTA GAAAGTCAAG CTTTGACAAT GTGGGACCCA GCACATCGCG TCATGCGGCC 
TCCGTTGTTG GCTTTGGAGG CGCCCAGCCA CCACCAGCGC CCAAGTTTCT GGGGCAAAAG 
AAACATCCGG AGCCGGCACC CAAGCTCTCC TTTCAGATTA AGAAACGACC GCTGCAGATG 
GACAAGCTGG GAATGCTGCG TCTAGCAGCG CCAGCGGAGC CACTCAAGGG AGGACGGAGC 
AGCAGCTCCC GCAGCCGGAG TAGGAGTAGA AGCCGCTCGC GAAGTCGCTC ACGTAATCGT 
CGGCTTGGAA GGAGCACGCG TTCGTCGCTC AGGAGCCGGC GTTCTGGCTC GAGGTCCAGA 
TCGATTAGCC GATCACGGTC GCGTCGCCGA CGTACCTACT CCCGTTCGCG CAGCCCCTCG 
CCACGCAGTT ACGGAAATCG CTTTGTGATT GGACGCTACC GTAATCGCCG AACTCGGCGT 
TCGCGCTCCT TTCATCGTCG GCGTTCTCCA TCGCCCATTT TGAAACGCCG ATCGCCTTCA 
CCATTTGTGC CCCGTCGCAC ACCATCGCCG GCATCCCGCT ATCGACGCAG CCGATCCCGC 
AGTCGCTCCC GCTCACGCAG TCGGAGTCCT CGACGCTTTC AGTCTCGCTA CATGCCGAGG 
GATCGTGGCT CCGTGAATCG AAGCAGGTAC TCCTGGCACA ATCCCCATAA ACGCAGTGTA 
TCACGCACCG TGGAGCCAAA TGGCAAAGAT GAAAGGGCAG CTACTCCGCC GAAAAAGGGT 
GTTGCTGATG ATAGATCTGG ACGAGAAGAA AGGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG6621 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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