Mcm5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mcm5
Entrez Gene ID	41296
Full Name	Minichromosome maintenance 5
Synonyms	CG4082,DmCDC46,DmCDC465,DmMCM5,DmMcm5,DmeMCM5,Dmel\CG4082,MCM5,McM5,PCR4,mcm5
General protein information	Preferred Names Minichromosome maintenance 5 Names CG4082 gene product from transcript CG4082-RA CG4082-PA Mcm5-PA mini-chromosome maintenance 5 minichromosome maintenance 5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86C6-86C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_079584.4	NP_524308.2	minichromosome maintenance 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	MCM5	NP_006730.2
Rattus norvegicus (Norway rat)	Mcm5	NP_001099640.1
Oryza sativa (rice)	Os02g0797400	NP_001048396.1
Pan troglodytes (chimpanzee)	MCM5	XP_001156025.1
Canis lupus familiaris (dog)	MCM5	XP_005626282.1
Caenorhabditis elegans (roundworm)	mcm-5	NP_497858.1
Saccharomyces cerevisiae (baker's yeast)	MCM5	NP_013376.1
Neurospora crassa	NCU01171	XP_961537.1
Xenopus tropicalis (tropical clawed frog)	mcm5	NP_001017327.2
Bos taurus (cattle)	MCM5	NP_001068758.1
Mus musculus (house mouse)	Mcm5	NP_032592.2
Gallus gallus (chicken)	MCM5	NP_001006243.1
Drosophila melanogaster (fruit fly)	Mcm5	NP_524308.2
Kluyveromyces lactis	KLLA0D09262g	XP_453475.1
Schizosaccharomyces pombe (fission yeast)	mcm5	XP_001713071.1
Danio rerio (zebrafish)	mcm5	NP_848523.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004408	XP_313694.2
Eremothecium gossypii	AGOS_AGR276W	NP_986942.1
Magnaporthe oryzae (rice blast fungus)	MGG_08646	XP_003711077.1
Arabidopsis thaliana (thale cress)	MCM5	NP_178812.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the decrease in recombination observed in females homozygous for mcm5(A7) is not due to a failure to create or repair meiotically induced double strand breaks (DSBs), but rather to a failure to resolve those DSBs into meiotic crossovers
Title: A genetic analysis of the Drosophila mcm5 gene defines a domain specifically required for meiotic recombination.

The following Mcm5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mcm5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08297	NM_079584.4 Latest version!	Drosophila melanogaster minichromosome maintenance 5 (Mcm5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mcm5 ( NM_079584.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524308.2 Drosophila melanogaster minichromosome maintenance 5 (Mcm5), mRNA.

Price & Availability↑

CloneID	OFa08297
Clone ID Related Accession (Same CDS sequence)	NM_079584.4
Accession Version	NM_079584.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	minichromosome maintenance 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079584.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGGCT TCGACGATGC GGGTGTGTTC TTTTCGGACA ATTTCGGCGG AGATAACCAG 
CAGGATGCGC AAATCAACTT GCAGGCGGTA AAGAAGAAGT ACAAGGAGTT CATCCGGACA 
TTTAACGAGG AGAACTTTTT CTACAAATAC CGTGACACCC TGAAGAGGAA CTATCTTAAT 
GGTCGCTACT TCTTGGAGAT TGAGATGGAG GATTTGGTGG GCTTCGATGA GACCTTGGCT 
GACAAGCTGA ACAAGCAGCC GACGGAGCAT TTGGAAATCT TCGAGGAGGC TGCCCGGGAG 
GTAGCCGACG AGATCACAGC TCCCCGGCCG GAGCACGAGG AACATATGCA CGACATCCAG 
ATTCTTCTGA GCTCCAATGC GAATCCCACC AACATACGAC AGTTGAAGTC GGACTGCGTA 
TCCAAGCTGG TTAAGATCGC CGGAATCATT GTCGCAGCCT CAGGAATAAG TGCCAAGGCT 
ACGCGGATGT CTATCCAATG CCTGTCCTGC AGCACGGTCA TACCGAATCT CAAAGTGAAT 
CCAGGTTTGG AGGGCTACGC CCTACCCAGG AAGTGCAACA CGGAGCAGGC GGGCAGACCC 
AAGTGCCCGC TGGACCCCTT CTTCATCATG CCGGACAAGT GCAAGTGCGT CGATTTCCAG 
ACCCTTAAGT TGCAAGAGCT CCCCGACTTT GTGCCACAGG GTGAGATTCC CCGCCACTTG 
CAGCTCTTCT GCGATCGCTC GCTCTGTGAA CGCGTGGTGC CCGGCAATCG CGTGCTTATC 
CAGGGCATAT ACTCCATTCG CAAGGTGGGC AAACCCTCGC GTCGGGATGG TCGCGAGAAA 
GCCGTCGTTG GAGTCCGTGC TCCTTACATG AGGGTGGTGG GCATCACCGT CGACTCAGAG 
GGTGCGGGTG CCATCTCCCG ATACAGCAAC ATCACATCCG ATGAGGAGGA GCACTTCCGG 
CGAATGGCCG CCTCTGGGGA CATCTACGAA CGTCTCTCAC AGTCACTGGC GCCCAGCATC 
TTCGGCTCTC GGGATATCAA GAAAGCTATA ACTTGTATGC TTTTTGGCGG GTCTCGTAAG 
CGTTTACCGG ATGGTCTGTG CCGTCGAGGC GACATTAACG TCTTACTGCT GGGAGATCCT 
GGTACTGCCA AGTCACAGTT GTTAAAGTTT GTGGAGAAGG TGGCACCCAT AGCAGTCTAC 
ACTTCGGGCA AAGGCTCCAG TGCGGCTGGT CTCACGGCTT CGGTTATGAA GGATCCTCAA 
ACGCGTAACT TTGTCATGGA GGGTGGAGCC ATGGTGCTGG CCGATGGAGG CGTGGTTTGT 
ATTGATGAGT TCGACAAAAT GCGAGAGGAT GATCGTGTGG CCATTCACGA GGCCATGGAA 
CAGCAGACAA TATCCATTGC CAAAGCCGGC ATCACGACCA CCTTGAACTC ACGCTGTTCG 
GTCTTGGCAG CTGCCAACTC CATTTTCGGT CGATGGGACG ACACCAAGGG CGAGGAGAAC 
ATTGACTTTA TGCCCACAAT TCTCTCCCGT TTCGACATGA TTTTCATTGT AAAGGATATT 
CACGATGAGT CGCGGGACAT TACGCTGGCC AAGCACATCA TCAACGTGCA TCTGAGCTCT 
AACAAGTCAG CGCCTTCAGA GCCGGCTGAG GGTGAGATAT CGCTGTCGAC GTTCAAGAAG 
TATATTCACT ACTGCCGAAC TCATTGCGGT CCGCGCCTGA GCGAAGCTGC CGGCGAGAAG 
CTAAAGAGCC GCTACGTGCT TATGCGCAGT GGAGCCGGCC AGCAGGAGAA GGCATCTGAC 
AAGCGGCTAA GCATTCCCAT CACTGTGCGT CAGTTGGAGG CTGTCATCCG CATTTCGGAG 
TCGCTGGCAA AGATTCGTTT GCAACCGTTC GCCACAGATG AGCACGTGAA CGAAGCACTT 
CGACTTTTCC AAGTGTCTAC CCTTGATGCT GCGATGACTG GCAGCTTGGC TGGAGCCGAA 
GGATTCACGA CCGAGGAGGA CCAGGAAACA CTCAACCGCA TCGAAAAGCA ATTGAAACGT 
CGCTTTGCAA TTGGATCTCA AGTATCGGAG CAGAATATCT TACAGGACTT TTTGCGTCAG 
AAATACGAAG AACGCACGGT AATGAAGGTA ATTCATACCA TGATCCGACG TGGAGAGCTG 
CAGCACCGGA TGCAACGGAA GATGCTCTAT CGAATTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524308.2
CDS	142..2343
Translation	MEGFDDAGVFFSDNFGGDNQQDAQINLQAVKKKYKEFIRTFNEENFFYKYRDTLKRNYLNGRYFLEIEMEDLVGFDETLADKLNKQPTEHLEIFEEAAREVADEITAPRPEHEEHMHDIQILLSSNANPTNIRQLKSDCVSKLVKIAGIIVAASGISAKATRMSIQCLSCSTVIPNLKVNPGLEGYALPRKCNTEQAGRPKCPLDPFFIMPDKCKCVDFQTLKLQELPDFVPQGEIPRHLQLFCDRSLCERVVPGNRVLIQGIYSIRKVGKPSRRDGREKAVVGVRAPYMRVVGITVDSEGAGAISRYSNITSDEEEHFRRMAASGDIYERLSQSLAPSIFGSRDIKKAITCMLFGGSRKRLPDGLCRRGDINVLLLGDPGTAKSQLLKFVEKVAPIAVYTSGKGSSAAGLTASVMKDPQTRNFVMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSIFGRWDDTKGEENIDFMPTILSRFDMIFIVKDIHDESRDITLAKHIINVHLSSNKSAPSEPAEGEISLSTFKKYIHYCRTHCGPRLSEAAGEKLKSRYVLMRSGAGQQEKASDKRLSIPITVRQLEAVIRISESLAKIRLQPFATDEHVNEALRLFQVSTLDAAMTGSLAGAEGFTTEEDQETLNRIEKQLKRRFAIGSQVSEQNILQDFLRQKYEERTVMKVIHTMIRRGELQHRMQRKMLYRIC

Target ORF information:

RefSeq Version	NM_079584.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster minichromosome maintenance 5 (Mcm5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079584.4

ORF Insert Sequence:

ATGGAAGGCT TCGACGATGC GGGTGTGTTC TTTTCGGACA ATTTCGGCGG AGATAACCAG 
CAGGATGCGC AAATCAACTT GCAGGCGGTA AAGAAGAAGT ACAAGGAGTT CATCCGGACA 
TTTAACGAGG AGAACTTTTT CTACAAATAC CGTGACACCC TGAAGAGGAA CTATCTTAAT 
GGTCGCTACT TCTTGGAGAT TGAGATGGAG GATTTGGTGG GCTTCGATGA GACCTTGGCT 
GACAAGCTGA ACAAGCAGCC GACGGAGCAT TTGGAAATCT TCGAGGAGGC TGCCCGGGAG 
GTAGCCGACG AGATCACAGC TCCCCGGCCG GAGCACGAGG AACATATGCA CGACATCCAG 
ATTCTTCTGA GCTCCAATGC GAATCCCACC AACATACGAC AGTTGAAGTC GGACTGCGTA 
TCCAAGCTGG TTAAGATCGC CGGAATCATT GTCGCAGCCT CAGGAATAAG TGCCAAGGCT 
ACGCGGATGT CTATCCAATG CCTGTCCTGC AGCACGGTCA TACCGAATCT CAAAGTGAAT 
CCAGGTTTGG AGGGCTACGC CCTACCCAGG AAGTGCAACA CGGAGCAGGC GGGCAGACCC 
AAGTGCCCGC TGGACCCCTT CTTCATCATG CCGGACAAGT GCAAGTGCGT CGATTTCCAG 
ACCCTTAAGT TGCAAGAGCT CCCCGACTTT GTGCCACAGG GTGAGATTCC CCGCCACTTG 
CAGCTCTTCT GCGATCGCTC GCTCTGTGAA CGCGTGGTGC CCGGCAATCG CGTGCTTATC 
CAGGGCATAT ACTCCATTCG CAAGGTGGGC AAACCCTCGC GTCGGGATGG TCGCGAGAAA 
GCCGTCGTTG GAGTCCGTGC TCCTTACATG AGGGTGGTGG GCATCACCGT CGACTCAGAG 
GGTGCGGGTG CCATCTCCCG ATACAGCAAC ATCACATCCG ATGAGGAGGA GCACTTCCGG 
CGAATGGCCG CCTCTGGGGA CATCTACGAA CGTCTCTCAC AGTCACTGGC GCCCAGCATC 
TTCGGCTCTC GGGATATCAA GAAAGCTATA ACTTGTATGC TTTTTGGCGG GTCTCGTAAG 
CGTTTACCGG ATGGTCTGTG CCGTCGAGGC GACATTAACG TCTTACTGCT GGGAGATCCT 
GGTACTGCCA AGTCACAGTT GTTAAAGTTT GTGGAGAAGG TGGCACCCAT AGCAGTCTAC 
ACTTCGGGCA AAGGCTCCAG TGCGGCTGGT CTCACGGCTT CGGTTATGAA GGATCCTCAA 
ACGCGTAACT TTGTCATGGA GGGTGGAGCC ATGGTGCTGG CCGATGGAGG CGTGGTTTGT 
ATTGATGAGT TCGACAAAAT GCGAGAGGAT GATCGTGTGG CCATTCACGA GGCCATGGAA 
CAGCAGACAA TATCCATTGC CAAAGCCGGC ATCACGACCA CCTTGAACTC ACGCTGTTCG 
GTCTTGGCAG CTGCCAACTC CATTTTCGGT CGATGGGACG ACACCAAGGG CGAGGAGAAC 
ATTGACTTTA TGCCCACAAT TCTCTCCCGT TTCGACATGA TTTTCATTGT AAAGGATATT 
CACGATGAGT CGCGGGACAT TACGCTGGCC AAGCACATCA TCAACGTGCA TCTGAGCTCT 
AACAAGTCAG CGCCTTCAGA GCCGGCTGAG GGTGAGATAT CGCTGTCGAC GTTCAAGAAG 
TATATTCACT ACTGCCGAAC TCATTGCGGT CCGCGCCTGA GCGAAGCTGC CGGCGAGAAG 
CTAAAGAGCC GCTACGTGCT TATGCGCAGT GGAGCCGGCC AGCAGGAGAA GGCATCTGAC 
AAGCGGCTAA GCATTCCCAT CACTGTGCGT CAGTTGGAGG CTGTCATCCG CATTTCGGAG 
TCGCTGGCAA AGATTCGTTT GCAACCGTTC GCCACAGATG AGCACGTGAA CGAAGCACTT 
CGACTTTTCC AAGTGTCTAC CCTTGATGCT GCGATGACTG GCAGCTTGGC TGGAGCCGAA 
GGATTCACGA CCGAGGAGGA CCAGGAAACA CTCAACCGCA TCGAAAAGCA ATTGAAACGT 
CGCTTTGCAA TTGGATCTCA AGTATCGGAG CAGAATATCT TACAGGACTT TTTGCGTCAG 
AAATACGAAG AACGCACGGT AATGAAGGTA ATTCATACCA TGATCCGACG TGGAGAGCTG 
CAGCACCGGA TGCAACGGAA GATGCTCTAT CGAATTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mcm5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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