hth cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hth
Entrez Gene ID	41273
Full Name	homothorax
Synonyms	1323/07,1422/04,CG17117,Dm-HTH,Dmel\CG17117,HTH,Hth,Meis1,P53,anon-EST:Liang-2.13,clone 2.13,dtl,hth1,hth2,l(3)05745,l(3)86Ca
General protein information	Preferred Names homothorax Names CG17117 gene product from transcript CG17117-RC CG17117-PA CG17117-PC CG17117-PE CG17117-PF CG17117-PI dorsotonals gene II hth-PA hth-PC hth-PE hth-PF hth-PI
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86C1-86C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_057228.5	NP_476576.1	homothorax, isoform C
NM_001014613.3	NP_001014613.2	homothorax, isoform E
NM_057230.6	NP_476578.3	homothorax, isoform A
NM_057229.5	NP_476577.3	homothorax, isoform I
NM_001031999.2	NP_001027170.1	homothorax, isoform F

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MEIS1	XP_001166938.1
	MEIS1	XP_001092652.1
Mus musculus (house mouse)	Meis1	NP_034919.1
Danio rerio (zebrafish)	meis1	NP_571968.1
Rattus norvegicus (Norway rat)	Meis1	NP_001128174.1
Drosophila melanogaster (fruit fly)	hth	NP_476578.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002178	XP_308010.5
Xenopus tropicalis (tropical clawed frog)	meis1	XP_002931793.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hth+Tsh-induced tissue overgrowth requires the BMP2 Dpp and the abnormal hyperactivation of its pathway. Rather than using autocrine Dpp expression, Hth+Tsh cells increase their avidity for Dpp, produced locally, by upregulating extracellular matrix components.
Title: Increased avidity for Dpp/BMP2 maintains the proliferation of progenitors-like cells in the Drosophila eye.

The retinal determination gene Dachshund restricts cell proliferation by limiting the activity of the Homothorax-Yorkie complex
Title: The retinal determination gene Dachshund restricts cell proliferation by limiting the activity of the Homothorax-Yorkie complex.

the satellite repeats get transcribed in wild type embryos and that this transcription depends on the presence of Hth, which binds to them as well as to the ribosomal DNA region.
Title: The TALE transcription factor homothorax functions to assemble heterochromatin during Drosophila embryogenesis.

Hth functions together with its co-factor Extradenticle to repress the R8-specific factor Senseless in dorsal rim area R8 cells, allowing expression of an ultraviolet-sensitive R7 Rhodopsin.
Title: Homothorax and Extradenticle alter the transcription factor network in Drosophila ommatidia at the dorsal rim of the retina.

Functional dissection of the splice variants of the Drosophila gene homothorax (hth
Title: Functional dissection of the splice variants of the Drosophila gene homothorax (hth).

The following hth gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hth cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08260	NM_057230.6 Latest version!	Drosophila melanogaster homothorax (hth), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08262	NM_001014613.3 Latest version!	Drosophila melanogaster homothorax (hth), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08263	NM_001031999.2 Latest version!	Drosophila melanogaster homothorax (hth), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08263	NM_057229.5 Latest version!	Drosophila melanogaster homothorax (hth), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08322	NM_057228.5 Latest version!	Drosophila melanogaster homothorax (hth), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hth ( NM_057230.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476578.3 Drosophila melanogaster homothorax (hth), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa08260
Clone ID Related Accession (Same CDS sequence)	NM_057230.6
Accession Version	NM_057230.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	homothorax, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057230.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGC CCAGGTATGA CGATGGCCTA CACGGCTACG GCATGGACTC CGGAGCCGCA 
GCAGCGGCCA TGTACGATCC ACACGCCGGT CACCGGCCGC CCGGACTGCA GGGCCTCCCC 
TCGCACCACT CTCCGCACAT GACGCACGCA GCGGCGGCGG CGGCCACAGT GGGCATGCAC 
GGCTACCATT CGGGGGCCGG GGGTCATGGA ACACCTAGTC ATGTATCGCC GGTCGGTAAT 
CACCTAATGG GCGCAATACC CGAAGTACAC AAACGTGATA AGGATGCGAT TTATGAACAT 
CCGCTATTCC CGCTTTTGGC GCTGATCTTT GAGAAGTGCG AATTGGCCAC ATGTACGCCG 
AGGGAGCCCG GTGTGCAAGG TGGCGATGTC TGTTCGTCGG AATCGTTCAA CGAGGATATT 
GCAATGTTCA GTAAACAGAT AAGATCACAG AAACCCTATT ATACCGCAGA TCCCGAAGTC 
GACTCACTGA TGGTGCAAGC AATACAAGTA CTTCGGTTTC ACCTTTTAGA ATTAGAAAAA 
GTACACGAGT TATGCGATAA CTTCTGTCAT CGGTATATAT CGTGTTTAAA GGGTAAAATG 
CCAATAGATT TAGTGATCGA CGAACGGGAC ACCACCAAAC CACCGGAGTT GGGATCGGCG 
AACGGAGAAG GGCGCAGCAA CGCCGACTCC ACATCGCACA CCGATGGAGC TAGTACACCA 
GACGTTCGGC CGCCGAGCTC ATCGCTGTCC TACGGCGGCG CAATGAACGA TGACGCCCGA 
TCGCCGGGCG CTGGTAGTAC TCCCGGTCCA CTGTCACAGC AGCCACCTGC CCTAGATACA 
TCAGACCCTG ATGGACGATG GTGTCGAAGA GAATGGTCCT CACCTGCCGA TGCTCGCAAT 
GCAGACGCTT CCCGGCGATT GTATTCCTCA GTCTTCCTCG GTAGTCCTGA CAATTTCGGA 
ACGAGTGCAA GCGGTGATGC CAGCAATGCC AGCATAGGAA GTGGAGAGGG CACCGGCGAG 
GAGGACGACG ATGCGAGCGG CAAAAAGAAC CAAAAGAAAC GTGGCATTTT CCCAAAAGTA 
GCAACCAACA TATTGAGAGC GTGGCTGTTT CAGCATTTAA CGCATCCCTA CCCATCCGAG 
GACCAGAAGA AACAATTGGC CCAGGACACC GGCCTAACGA TACTGCAAGT GAATAATTGG 
TTCATCAATG CGCGGCGGAG AATTGTCCAG CCAATGATCG ATCAATCGAA TCGTGCAGTC 
TATACACCGC ATCCAGGTCC CTCCGGATAT GGCCACGACG CCATGGGCTA CATGATGGAC 
AGCCAGGCGC ATATGATGCA CCGTCCGCCC GGAGATCCGG GCTTCCACCA GGGCTATCCG 
CATTACCCGC CCGCCGAGTA CTACGGCCAG CACTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476578.3
CDS	1278..2696
Translation	MAQPRYDDGLHGYGMDSGAAAAAMYDPHAGHRPPGLQGLPSHHSPHMTHAAAAAATVGMHGYHSGAGGHGTPSHVSPVGNHLMGAIPEVHKRDKDAIYEHPLFPLLALIFEKCELATCTPREPGVQGGDVCSSESFNEDIAMFSKQIRSQKPYYTADPEVDSLMVQAIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIDLVIDERDTTKPPELGSANGEGRSNADSTSHTDGASTPDVRPPSSSLSYGGAMNDDARSPGAGSTPGPLSQQPPALDTSDPDGRWCRREWSSPADARNADASRRLYSSVFLGSPDNFGTSASGDASNASIGSGEGTGEEDDDASGKKNQKKRGIFPKVATNILRAWLFQHLTHPYPSEDQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAVYTPHPGPSGYGHDAMGYMMDSQAHMMHRPPGDPGFHQGYPHYPPAEYYGQHL

Target ORF information:

RefSeq Version	NM_057230.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster homothorax (hth), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057230.6

ORF Insert Sequence:

ATGGCTCAGC CCAGGTATGA CGATGGCCTA CACGGCTACG GCATGGACTC CGGAGCCGCA 
GCAGCGGCCA TGTACGATCC ACACGCCGGT CACCGGCCGC CCGGACTGCA GGGCCTCCCC 
TCGCACCACT CTCCGCACAT GACGCACGCA GCGGCGGCGG CGGCCACAGT GGGCATGCAC 
GGCTACCATT CGGGGGCCGG GGGTCATGGA ACACCTAGTC ATGTATCGCC GGTCGGTAAT 
CACCTAATGG GCGCAATACC CGAAGTACAC AAACGTGATA AGGATGCGAT TTATGAACAT 
CCGCTATTCC CGCTTTTGGC GCTGATCTTT GAGAAGTGCG AATTGGCCAC ATGTACGCCG 
AGGGAGCCCG GTGTGCAAGG TGGCGATGTC TGTTCGTCGG AATCGTTCAA CGAGGATATT 
GCAATGTTCA GTAAACAGAT AAGATCACAG AAACCCTATT ATACCGCAGA TCCCGAAGTC 
GACTCACTGA TGGTGCAAGC AATACAAGTA CTTCGGTTTC ACCTTTTAGA ATTAGAAAAA 
GTACACGAGT TATGCGATAA CTTCTGTCAT CGGTATATAT CGTGTTTAAA GGGTAAAATG 
CCAATAGATT TAGTGATCGA CGAACGGGAC ACCACCAAAC CACCGGAGTT GGGATCGGCG 
AACGGAGAAG GGCGCAGCAA CGCCGACTCC ACATCGCACA CCGATGGAGC TAGTACACCA 
GACGTTCGGC CGCCGAGCTC ATCGCTGTCC TACGGCGGCG CAATGAACGA TGACGCCCGA 
TCGCCGGGCG CTGGTAGTAC TCCCGGTCCA CTGTCACAGC AGCCACCTGC CCTAGATACA 
TCAGACCCTG ATGGACGATG GTGTCGAAGA GAATGGTCCT CACCTGCCGA TGCTCGCAAT 
GCAGACGCTT CCCGGCGATT GTATTCCTCA GTCTTCCTCG GTAGTCCTGA CAATTTCGGA 
ACGAGTGCAA GCGGTGATGC CAGCAATGCC AGCATAGGAA GTGGAGAGGG CACCGGCGAG 
GAGGACGACG ATGCGAGCGG CAAAAAGAAC CAAAAGAAAC GTGGCATTTT CCCAAAAGTA 
GCAACCAACA TATTGAGAGC GTGGCTGTTT CAGCATTTAA CGCATCCCTA CCCATCCGAG 
GACCAGAAGA AACAATTGGC CCAGGACACC GGCCTAACGA TACTGCAAGT GAATAATTGG 
TTCATCAATG CGCGGCGGAG AATTGTCCAG CCAATGATCG ATCAATCGAA TCGTGCAGTC 
TATACACCGC ATCCAGGTCC CTCCGGATAT GGCCACGACG CCATGGGCTA CATGATGGAC 
AGCCAGGCGC ATATGATGCA CCGTCCGCCC GGAGATCCGG GCTTCCACCA GGGCTATCCG 
CATTACCCGC CCGCCGAGTA CTACGGCCAG CACTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hth ( NM_001014613.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014613.2 Drosophila melanogaster homothorax (hth), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa08262
Clone ID Related Accession (Same CDS sequence)	NM_001014613.3
Accession Version	NM_001014613.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	homothorax, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001014613.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGC CCAGGTATGA CGATGGCCTA CACGGCTACG GCATGGACTC CGGAGCCGCA 
GCAGCGGCCA TGTACGATCC ACACGCCGGT CACCGGCCGC CCGGACTGCA GGGCCTCCCC 
TCGCACCACT CTCCGCACAT GACGCACGCA GCGGCGGCGG CGGCCACAGT GGGCATGCAC 
GGCTACCATT CGGGGGCCGG GGGTCATGGA ACACCTAGTC ATGTATCGCC GGTCGGTAAT 
CACCTAATGG GCGCAATACC CGAAGTACAC AAACGTGATA AGGATGCGAT TTATGAACAT 
CCGCTATTCC CGCTTTTGGC GCTGATCTTT GAGAAGTGCG AATTGGCCAC ATGTACGCCG 
AGGGAGCCCG GTGTGCAAGG TGGCGATGTC TGTTCGTCGG AATCGTTCAA CGAGGATATT 
GCAATGTTCA GTAAACAGAT AAGATCACAG AAACCCTATT ATACCGCAGA TCCCGAAGTC 
GACTCACTGA TGGTGCAAGC AATACAAGTA CTTCGGTTTC ACCTTTTAGA ATTAGAAAAA 
GTACACGAGT TATGCGATAA CTTCTGTCAT CGGTATATAT CGTGTTTAAA GGGTAAAATG 
CCAATAGATT TAGTGATCGA CGAACGGGAC ACCACCAAAC CACCGGAGTT GGGATCGGCG 
AACGGAGAAG GGCGCAGCAA CGCCGACTCC ACATCGCACA CCGATGGAGC TAGTACACCA 
GACGTTCCCA GCACCCAAGA CTTTTCACCG CTGGAGGAGA CCTATGCCAG CTATCGCATC 
AAACACGAGG CGGACTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014613.2
CDS	1747..2547
Translation	MAQPRYDDGLHGYGMDSGAAAAAMYDPHAGHRPPGLQGLPSHHSPHMTHAAAAAATVGMHGYHSGAGGHGTPSHVSPVGNHLMGAIPEVHKRDKDAIYEHPLFPLLALIFEKCELATCTPREPGVQGGDVCSSESFNEDIAMFSKQIRSQKPYYTADPEVDSLMVQAIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIDLVIDERDTTKPPELGSANGEGRSNADSTSHTDGASTPDVPSTQDFSPLEETYASYRIKHEADF

Target ORF information:

RefSeq Version	NM_001014613.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster homothorax (hth), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014613.3

ORF Insert Sequence:

ATGGCTCAGC CCAGGTATGA CGATGGCCTA CACGGCTACG GCATGGACTC CGGAGCCGCA 
GCAGCGGCCA TGTACGATCC ACACGCCGGT CACCGGCCGC CCGGACTGCA GGGCCTCCCC 
TCGCACCACT CTCCGCACAT GACGCACGCA GCGGCGGCGG CGGCCACAGT GGGCATGCAC 
GGCTACCATT CGGGGGCCGG GGGTCATGGA ACACCTAGTC ATGTATCGCC GGTCGGTAAT 
CACCTAATGG GCGCAATACC CGAAGTACAC AAACGTGATA AGGATGCGAT TTATGAACAT 
CCGCTATTCC CGCTTTTGGC GCTGATCTTT GAGAAGTGCG AATTGGCCAC ATGTACGCCG 
AGGGAGCCCG GTGTGCAAGG TGGCGATGTC TGTTCGTCGG AATCGTTCAA CGAGGATATT 
GCAATGTTCA GTAAACAGAT AAGATCACAG AAACCCTATT ATACCGCAGA TCCCGAAGTC 
GACTCACTGA TGGTGCAAGC AATACAAGTA CTTCGGTTTC ACCTTTTAGA ATTAGAAAAA 
GTACACGAGT TATGCGATAA CTTCTGTCAT CGGTATATAT CGTGTTTAAA GGGTAAAATG 
CCAATAGATT TAGTGATCGA CGAACGGGAC ACCACCAAAC CACCGGAGTT GGGATCGGCG 
AACGGAGAAG GGCGCAGCAA CGCCGACTCC ACATCGCACA CCGATGGAGC TAGTACACCA 
GACGTTCCCA GCACCCAAGA CTTTTCACCG CTGGAGGAGA CCTATGCCAG CTATCGCATC 
AAACACGAGG CGGACTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hth ( NM_057229.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476577.3 Drosophila melanogaster homothorax (hth), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa08263
Clone ID Related Accession (Same CDS sequence)	NM_057229.5 , NM_001031999.2
Accession Version	NM_057229.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	homothorax, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057229.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGC CCAGGTATGA CGATGGCCTA CACGGCTACG GCATGGACTC CGGAGCCGCA 
GCAGCGGCCA TGTACGATCC ACACGCCGGT CACCGGCCGC CCGGACTGCA GGGCCTCCCC 
TCGCACCACT CTCCGCACAT GACGCACGCA GCGGCGGCGG CGGCCACAGT GGGCATGCAC 
GGCTACCATT CGGGGGCCGG GGGTCATGGA ACACCTAGTC ATGTATCGCC GGTCGGTAAT 
CACCTAATGG GCGCAATACC CGAAGTACAC AAACGTGATA AGGATGCGAT TTATGAACAT 
CCGCTATTCC CGCTTTTGGC GCTGATCTTT GAGAAGTGCG AATTGGCCAC ATGTACGCCG 
AGGGAGCCCG GTGTGCAAGG TGGCGATGTC TGTTCGTCGG AATCGTTCAA CGAGGATATT 
GCAATGTTCA GTAAACAGAT AAGATCACAG AAACCCTATT ATACCGCAGA TCCCGAAGTC 
GACTCACTGA TGGTGCAAGC AATACAAGTA CTTCGGTTTC ACCTTTTAGA ATTAGAAAAA 
GTACACGAGT TATGCGATAA CTTCTGTCAT CGGTATATAT CGTGTTTAAA GGGTAAAATG 
CCAATAGATT TAGTGATCGA CGAACGGGAC ACCACCAAAC CACCGGAGTT GGGATCGGCG 
AACGGAGAAG GGCGCAGCAA CGCCGACTCC ACATCGCACA CCGATGGAGC TAGTACACCA 
GACGTTGTGA GTACCCCCTT CGCGGGAGCA CATGGTCCTA TATTGGCGAG CTACAATGCG 
GTGGTGCACC CATGTTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476577.3
CDS	1278..2078
Translation	MAQPRYDDGLHGYGMDSGAAAAAMYDPHAGHRPPGLQGLPSHHSPHMTHAAAAAATVGMHGYHSGAGGHGTPSHVSPVGNHLMGAIPEVHKRDKDAIYEHPLFPLLALIFEKCELATCTPREPGVQGGDVCSSESFNEDIAMFSKQIRSQKPYYTADPEVDSLMVQAIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIDLVIDERDTTKPPELGSANGEGRSNADSTSHTDGASTPDVVSTPFAGAHGPILASYNAVVHPCS

Target ORF information:

RefSeq Version	NM_057229.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster homothorax (hth), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057229.5

ORF Insert Sequence:

ATGGCTCAGC CCAGGTATGA CGATGGCCTA CACGGCTACG GCATGGACTC CGGAGCCGCA 
GCAGCGGCCA TGTACGATCC ACACGCCGGT CACCGGCCGC CCGGACTGCA GGGCCTCCCC 
TCGCACCACT CTCCGCACAT GACGCACGCA GCGGCGGCGG CGGCCACAGT GGGCATGCAC 
GGCTACCATT CGGGGGCCGG GGGTCATGGA ACACCTAGTC ATGTATCGCC GGTCGGTAAT 
CACCTAATGG GCGCAATACC CGAAGTACAC AAACGTGATA AGGATGCGAT TTATGAACAT 
CCGCTATTCC CGCTTTTGGC GCTGATCTTT GAGAAGTGCG AATTGGCCAC ATGTACGCCG 
AGGGAGCCCG GTGTGCAAGG TGGCGATGTC TGTTCGTCGG AATCGTTCAA CGAGGATATT 
GCAATGTTCA GTAAACAGAT AAGATCACAG AAACCCTATT ATACCGCAGA TCCCGAAGTC 
GACTCACTGA TGGTGCAAGC AATACAAGTA CTTCGGTTTC ACCTTTTAGA ATTAGAAAAA 
GTACACGAGT TATGCGATAA CTTCTGTCAT CGGTATATAT CGTGTTTAAA GGGTAAAATG 
CCAATAGATT TAGTGATCGA CGAACGGGAC ACCACCAAAC CACCGGAGTT GGGATCGGCG 
AACGGAGAAG GGCGCAGCAA CGCCGACTCC ACATCGCACA CCGATGGAGC TAGTACACCA 
GACGTTGTGA GTACCCCCTT CGCGGGAGCA CATGGTCCTA TATTGGCGAG CTACAATGCG 
GTGGTGCACC CATGTTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hth ( NM_001031999.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027170.1 Drosophila melanogaster homothorax (hth), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa08263
Clone ID Related Accession (Same CDS sequence)	NM_057229.5 , NM_001031999.2
Accession Version	NM_001031999.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	homothorax, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001031999.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGC CCAGGTATGA CGATGGCCTA CACGGCTACG GCATGGACTC CGGAGCCGCA 
GCAGCGGCCA TGTACGATCC ACACGCCGGT CACCGGCCGC CCGGACTGCA GGGCCTCCCC 
TCGCACCACT CTCCGCACAT GACGCACGCA GCGGCGGCGG CGGCCACAGT GGGCATGCAC 
GGCTACCATT CGGGGGCCGG GGGTCATGGA ACACCTAGTC ATGTATCGCC GGTCGGTAAT 
CACCTAATGG GCGCAATACC CGAAGTACAC AAACGTGATA AGGATGCGAT TTATGAACAT 
CCGCTATTCC CGCTTTTGGC GCTGATCTTT GAGAAGTGCG AATTGGCCAC ATGTACGCCG 
AGGGAGCCCG GTGTGCAAGG TGGCGATGTC TGTTCGTCGG AATCGTTCAA CGAGGATATT 
GCAATGTTCA GTAAACAGAT AAGATCACAG AAACCCTATT ATACCGCAGA TCCCGAAGTC 
GACTCACTGA TGGTGCAAGC AATACAAGTA CTTCGGTTTC ACCTTTTAGA ATTAGAAAAA 
GTACACGAGT TATGCGATAA CTTCTGTCAT CGGTATATAT CGTGTTTAAA GGGTAAAATG 
CCAATAGATT TAGTGATCGA CGAACGGGAC ACCACCAAAC CACCGGAGTT GGGATCGGCG 
AACGGAGAAG GGCGCAGCAA CGCCGACTCC ACATCGCACA CCGATGGAGC TAGTACACCA 
GACGTTGTGA GTACCCCCTT CGCGGGAGCA CATGGTCCTA TATTGGCGAG CTACAATGCG 
GTGGTGCACC CATGTTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027170.1
CDS	1278..2078
Translation	MAQPRYDDGLHGYGMDSGAAAAAMYDPHAGHRPPGLQGLPSHHSPHMTHAAAAAATVGMHGYHSGAGGHGTPSHVSPVGNHLMGAIPEVHKRDKDAIYEHPLFPLLALIFEKCELATCTPREPGVQGGDVCSSESFNEDIAMFSKQIRSQKPYYTADPEVDSLMVQAIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIDLVIDERDTTKPPELGSANGEGRSNADSTSHTDGASTPDVVSTPFAGAHGPILASYNAVVHPCS

Target ORF information:

RefSeq Version	NM_001031999.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster homothorax (hth), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031999.2

ORF Insert Sequence:

ATGGCTCAGC CCAGGTATGA CGATGGCCTA CACGGCTACG GCATGGACTC CGGAGCCGCA 
GCAGCGGCCA TGTACGATCC ACACGCCGGT CACCGGCCGC CCGGACTGCA GGGCCTCCCC 
TCGCACCACT CTCCGCACAT GACGCACGCA GCGGCGGCGG CGGCCACAGT GGGCATGCAC 
GGCTACCATT CGGGGGCCGG GGGTCATGGA ACACCTAGTC ATGTATCGCC GGTCGGTAAT 
CACCTAATGG GCGCAATACC CGAAGTACAC AAACGTGATA AGGATGCGAT TTATGAACAT 
CCGCTATTCC CGCTTTTGGC GCTGATCTTT GAGAAGTGCG AATTGGCCAC ATGTACGCCG 
AGGGAGCCCG GTGTGCAAGG TGGCGATGTC TGTTCGTCGG AATCGTTCAA CGAGGATATT 
GCAATGTTCA GTAAACAGAT AAGATCACAG AAACCCTATT ATACCGCAGA TCCCGAAGTC 
GACTCACTGA TGGTGCAAGC AATACAAGTA CTTCGGTTTC ACCTTTTAGA ATTAGAAAAA 
GTACACGAGT TATGCGATAA CTTCTGTCAT CGGTATATAT CGTGTTTAAA GGGTAAAATG 
CCAATAGATT TAGTGATCGA CGAACGGGAC ACCACCAAAC CACCGGAGTT GGGATCGGCG 
AACGGAGAAG GGCGCAGCAA CGCCGACTCC ACATCGCACA CCGATGGAGC TAGTACACCA 
GACGTTGTGA GTACCCCCTT CGCGGGAGCA CATGGTCCTA TATTGGCGAG CTACAATGCG 
GTGGTGCACC CATGTTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hth ( NM_057228.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476576.1 Drosophila melanogaster homothorax (hth), transcript variant C, mRNA.

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CloneID	OFa08322
Clone ID Related Accession (Same CDS sequence)	NM_057228.5
Accession Version	NM_057228.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	homothorax, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057228.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGC CCAGGTATGA CGATGGCCTA CACGGCTACG GCATGGACTC CGGAGCCGCA 
GCAGCGGCCA TGTACGATCC ACACGCCGGT CACCGGCCGC CCGGACTGCA GGGCCTCCCC 
TCGCACCACT CTCCGCACAT GACGCACGCA GCGGCGGCGG CGGCCACAGT GGGCATGCAC 
GGCTACCATT CGGGGGCCGG GGGTCATGGA ACACCTAGTC ATGTATCGCC GGTCGGTAAT 
CACCTAATGG GCGCAATACC CGAAGTACAC AAACGTGATA AGGATGCGAT TTATGAACAT 
CCGCTATTCC CGCTTTTGGC GCTGATCTTT GAGAAGTGCG AATTGGCCAC ATGTACGCCG 
AGGGAGCCCG GTGTGCAAGG TGGCGATGTC TGTTCGTCGG AATCGTTCAA CGAGGATATT 
GCAATGTTCA GTAAACAGAT AAGATCACAG AAACCCTATT ATACCGCAGA TCCCGAAGTC 
GACTCACTGA TGGTGCAAGC AATACAAGTA CTTCGGTTTC ACCTTTTAGA ATTAGAAAAA 
GTACACGAGT TATGCGATAA CTTCTGTCAT CGGTATATAT CGTGTTTAAA GGGTAAAATG 
CCAATAGATT TAGTGATCGA CGAACGGGAC ACCACCAAAC CACCGGAGTT GGGATCGGCG 
AACGGAGAAG GGCGCAGCAA CGCCGACTCC ACATCGCACA CCGATGGAGC TAGTACACCA 
GACGTTCGGC CGCCGAGCTC ATCGCTGTCC TACGGCGGCG CAATGAACGA TGACGCCCGA 
TCGCCGGGCG CTGGTAGTAC TCCCGGTCCA CTGTCACAGC AGCCACCTGC CCTAGATACA 
TCAGACCCTG ATGGTAAGTT CTTATCATCA CTCAATCCTT CAGAACTAAC ATATGATGGA 
CGATGGTGTC GAAGAGAATG GTCCTCACCT GCCGATGCTC GCAATGCAGA CGCTTCCCGG 
CGATTGTATT CCTCAGTCTT CCTCGGTAGT CCTGACAATT TCGGAACGAG TGCAAGCGGT 
GATGCCAGCA ATGCCAGCAT AGGAAGTGGA GAGGGCACCG GCGAGGAGGA CGACGATGCG 
AGCGGCAAAA AGAACCAAAA GAAACGTGGC ATTTTCCCAA AAGTAGCAAC CAACATATTG 
AGAGCGTGGC TGTTTCAGCA TTTAACGCAT CCCTACCCAT CCGAGGACCA GAAGAAACAA 
TTGGCCCAGG ACACCGGCCT AACGATACTG CAAGTGAATA ATTGGTTCAT CAATGCGCGG 
CGGAGAATTG TCCAGCCAAT GATCGATCAA TCGAATCGTG CAGTCTATAC ACCGCATCCA 
GGTCCCTCCG GATATGGCCA CGACGCCATG GGCTACATGA TGGACAGCCA GGCGCATATG 
ATGCACCGTC CGCCCGGAGA TCCGGGCTTC CACCAGGGCT ATCCGCATTA CCCGCCCGCC 
GAGTACTACG GCCAGCACTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476576.1
CDS	1278..2741
Translation	MAQPRYDDGLHGYGMDSGAAAAAMYDPHAGHRPPGLQGLPSHHSPHMTHAAAAAATVGMHGYHSGAGGHGTPSHVSPVGNHLMGAIPEVHKRDKDAIYEHPLFPLLALIFEKCELATCTPREPGVQGGDVCSSESFNEDIAMFSKQIRSQKPYYTADPEVDSLMVQAIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIDLVIDERDTTKPPELGSANGEGRSNADSTSHTDGASTPDVRPPSSSLSYGGAMNDDARSPGAGSTPGPLSQQPPALDTSDPDGKFLSSLNPSELTYDGRWCRREWSSPADARNADASRRLYSSVFLGSPDNFGTSASGDASNASIGSGEGTGEEDDDASGKKNQKKRGIFPKVATNILRAWLFQHLTHPYPSEDQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAVYTPHPGPSGYGHDAMGYMMDSQAHMMHRPPGDPGFHQGYPHYPPAEYYGQHL

Target ORF information:

RefSeq Version	NM_057228.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster homothorax (hth), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057228.5

ORF Insert Sequence:

ATGGCTCAGC CCAGGTATGA CGATGGCCTA CACGGCTACG GCATGGACTC CGGAGCCGCA 
GCAGCGGCCA TGTACGATCC ACACGCCGGT CACCGGCCGC CCGGACTGCA GGGCCTCCCC 
TCGCACCACT CTCCGCACAT GACGCACGCA GCGGCGGCGG CGGCCACAGT GGGCATGCAC 
GGCTACCATT CGGGGGCCGG GGGTCATGGA ACACCTAGTC ATGTATCGCC GGTCGGTAAT 
CACCTAATGG GCGCAATACC CGAAGTACAC AAACGTGATA AGGATGCGAT TTATGAACAT 
CCGCTATTCC CGCTTTTGGC GCTGATCTTT GAGAAGTGCG AATTGGCCAC ATGTACGCCG 
AGGGAGCCCG GTGTGCAAGG TGGCGATGTC TGTTCGTCGG AATCGTTCAA CGAGGATATT 
GCAATGTTCA GTAAACAGAT AAGATCACAG AAACCCTATT ATACCGCAGA TCCCGAAGTC 
GACTCACTGA TGGTGCAAGC AATACAAGTA CTTCGGTTTC ACCTTTTAGA ATTAGAAAAA 
GTACACGAGT TATGCGATAA CTTCTGTCAT CGGTATATAT CGTGTTTAAA GGGTAAAATG 
CCAATAGATT TAGTGATCGA CGAACGGGAC ACCACCAAAC CACCGGAGTT GGGATCGGCG 
AACGGAGAAG GGCGCAGCAA CGCCGACTCC ACATCGCACA CCGATGGAGC TAGTACACCA 
GACGTTCGGC CGCCGAGCTC ATCGCTGTCC TACGGCGGCG CAATGAACGA TGACGCCCGA 
TCGCCGGGCG CTGGTAGTAC TCCCGGTCCA CTGTCACAGC AGCCACCTGC CCTAGATACA 
TCAGACCCTG ATGGTAAGTT CTTATCATCA CTCAATCCTT CAGAACTAAC ATATGATGGA 
CGATGGTGTC GAAGAGAATG GTCCTCACCT GCCGATGCTC GCAATGCAGA CGCTTCCCGG 
CGATTGTATT CCTCAGTCTT CCTCGGTAGT CCTGACAATT TCGGAACGAG TGCAAGCGGT 
GATGCCAGCA ATGCCAGCAT AGGAAGTGGA GAGGGCACCG GCGAGGAGGA CGACGATGCG 
AGCGGCAAAA AGAACCAAAA GAAACGTGGC ATTTTCCCAA AAGTAGCAAC CAACATATTG 
AGAGCGTGGC TGTTTCAGCA TTTAACGCAT CCCTACCCAT CCGAGGACCA GAAGAAACAA 
TTGGCCCAGG ACACCGGCCT AACGATACTG CAAGTGAATA ATTGGTTCAT CAATGCGCGG 
CGGAGAATTG TCCAGCCAAT GATCGATCAA TCGAATCGTG CAGTCTATAC ACCGCATCCA 
GGTCCCTCCG GATATGGCCA CGACGCCATG GGCTACATGA TGGACAGCCA GGCGCATATG 
ATGCACCGTC CGCCCGGAGA TCCGGGCTTC CACCAGGGCT ATCCGCATTA CCCGCCCGCC 
GAGTACTACG GCCAGCACTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hth cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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