CG3999 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG3999
Entrez Gene ID	41253
Full Name	CG3999 gene product from transcript CG3999-RA
Synonyms	Dmel\CG3999
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86A2-86A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_141732.2	NP_649989.1	uncharacterized protein, isoform A
NM_001275527.1	NP_001262456.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLDC	NP_000161.2
Danio rerio (zebrafish)	gldc	NP_955848.1
Saccharomyces cerevisiae (baker's yeast)	GCV2	NP_013914.1
Magnaporthe oryzae (rice blast fungus)	MGG_15038	XP_003720581.1
Canis lupus familiaris (dog)	GLDC	XP_538655.2
Rattus norvegicus (Norway rat)	Gldc	NP_001101053.1
Caenorhabditis elegans (roundworm)	R12C12.1	NP_495209.1
Kluyveromyces lactis	KLLA0D12738g	XP_453630.1
Xenopus tropicalis (tropical clawed frog)	gldc	XP_002934225.2
Pan troglodytes (chimpanzee)	GLDC	XP_001143144.1
	GLDC	XP_001112651.1
Mus musculus (house mouse)	Gldc	NP_613061.1
Arabidopsis thaliana (thale cress)	GLDP2	NP_180178.1
Oryza sativa (rice)	Os01g0711400	NP_001044046.1
Drosophila melanogaster (fruit fly)	CG3999	NP_649989.1
Neurospora crassa	NCU02475	XP_965069.2
Bos taurus (cattle)	GLDC	NP_001179880.1
Gallus gallus (chicken)	GLDC	NP_989653.1
Schizosaccharomyces pombe (fission yeast)	gcv2	NP_592832.2
Arabidopsis thaliana (thale cress)	GLDP1	NP_195027.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG3999 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG3999 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04445	NM_141732.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG3999), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa04445	NM_001275527.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG3999), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG3999 ( NM_141732.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649989.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG3999), mRNA.

Price & Availability↑

CloneID	OFa04445
Clone ID Related Accession (Same CDS sequence)	NM_141732.2 , NM_001275527.1
Accession Version	NM_141732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141732.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCGAT TTTTGGGAAG GAGCAGCCAA AGCCTTTTGC TAAGATGTGG TCATCGCTAT 
CTGGCCACCA CTCCTGTGGA GGAGGTCATA TTTCCCACCA AATCGGATTT TCCCAGTCGT 
CATATTGGGC CTCGAAAGAC GGATGTGGTG GCCATGTTGG ATACTTTGGG CTTCAAGTCC 
CTGGCGGAGC TCACAGAGAA AGCTGTGCCC CAGAGCATTC AACTGAAGAG GGAACTCGAC 
CTGGACAAAC CTCTGAATGA GCACGAGCTA ATCCGCCGAA TTCGCGACAT CTCGCTAAAG 
AATCAACTGT GGCGCTCCTA CATCGGAATG GGTTACCACA ACTGCCATGT GCCGCACACC 
ATTATCCGGA ATATGTTCGA GAATCCGGGT TGGACCACTC AGTACACCCC GTACCAGCCG 
GAAATCGCCC AGGGACGCTT GGAGTCGCTG CTGAACTATC AGACCCTGGT AACCGATCTC 
ACCGGCTTGG ATGTGGCCAA TGCCTCGCTA TTGGACGAGG GAACCGCTGC TGCCGAGGCC 
ATGTGCCTGG CCACCAGGCA CAACAAGCGC AAAAAGCTCT ACCTGTCGAA CAGGGTGCAT 
CCCCAGACGC TGTCCGTAGT CAAGACGCGA ACGGAAGCCT TGGAGCTGGA AATCGTGGTG 
GGACCTATTG AACAGGCGGA TCTGCCAAGC CGCGAATTGG CTGGCATTCT TCTCCAGTAT 
CCAGATACTT ATGGTGATGT CAAGGACTTC GAGGATATCG CAGCTTTGGC AAAGAAAAAT 
GGGACCCTCG TGGTGGTGGC GACCGATTTG TTGTCGTTAA CCCTTCTGCG TCCTCCGGCG 
GAATTTGGAG CTGATATTGC AGTGGGAACC TCCCAGCGAT TGGGAGTTCC TCTGGGATAC 
GGTGGCCCCC ATGCCGGATT TTTTGCCTGC AAGCAGAGCT TGGTGCGGCT AATGCCGGGA 
AGGATGATTG GCGTGACCAG GGATATGGAC GGAAACGATG CCTATCGTCT GGCCCTGCAA 
ACCAGGGAGC AGCATATCCG GCGGGATAAG GCAACAAGTA ACATTTGCAC GGCACAGGCT 
CTTCTGGCCA ACATGTCGGC CATGTACGCC ATCTACCACG GTCCGGAGGG TCTGAAAGCC 
ATGGCCAATC GCATCCACCA TTTCACGTTG ACCCTTCAAA CGGGTGTCCT GGGAGCGGGT 
CACGAAGTGA TCAACAAGAA CTTCTTCGAC ACACTTCACA TTAGACTGGG TGGAGGTCAA 
TCCCTTGAGG ATCTCAAAGA GCGTGCCGAG CACAAGCGCA TTAATCTCCG ATATCTTGAA 
GATGACACTG TTGGCGTGGC CCTTGATGAA ACGGTCAGTG TAGCCGATGT GGACGATCTG 
TTGTGGGTCT TCAAGGCAGA GGCATCTGTG GAACACATTC TGGCGCGTAA AGATGTCCTT 
AAGAACTCGA TTGAGAACTC CAAGTTCCTG CGCACTTCGC CCTATCTGCA GCACCCTATT 
TTCCAGAGCT ATCACAGCGA GTCCCGCATG GTGCGCTACA TGAAAAAGCT GGAGAATAAG 
GACATCTCCC TGGTGCACTC CATGATCCCC CTGGGATCCT GCACTATGAA GCTGAACTCC 
ACCACCGAGA TGATGCCTTG TTCCTTCCGC CACTTCACCG ATATCCATCC TTTTGCGCCG 
GTGGAACAAG CCCAGGGATT CCATCAGATG TTCAAGGAAC TGGAGCATGA CCTATGCGAG 
ATTACAGGAT ACGATCGTAT CTCCTTCCAG CCGAACTCGG GGGCCCAAGG AGAATATGCC 
GGACTGAGAG CCATCAGGAG CTACCATGAA CACCGAAATG AAGGACACAG AAATATTTGT 
CTGATTCCAA TTTCTGCCCA CGGCACAAAT CCAGCTTCTG CACAAATGGC GGGTATGAAG 
GTGGAGCCTA TTAGAATTTT GTCGAATGGT TCCATCGACA TGGCACACCT AAGAGCTAAG 
GCAGAGGAGC ACGCCCACGA GTTGTCCTGC CTAATGATTA CCTATCCATC GACAATGGGC 
GTGTTCGAGG AAACGGTTGC CGATATTTGC ACCCTGATCC ACAAGCATGG CGGACAGGTG 
TACCTGGATG GAGCCAACAT GAATGCCCAG GTTGGGCTGT GTCGACCTGG AGATTATGGC 
AGCGATGTTT CGCATCTCAA CCTGCACAAG ACCTTCTGCA TTCCGCATGG TGGTGGTGGT 
CCTGGAATGG GTCCCATCGG CGTGAAAGCC CATTTGGCAC CATACCTTCC TGGTCATCCT 
GTTGTCAGTC CGTTGAGCAG CGAGGAGCAC AGTTTTGGAG TCGTATCCGC CGCTCCCTTC 
GGAAGCTCTG CCATTTTGCC CATATCCTGG TCGTACATTA AACTCATGGG CAGCAGGGGT 
TTGAAGAGAG CTACCCAGGT GGCCATTCTC AATGCAAACT ACATGTCCAA GAGGCTGGAG 
CAGCATTACA AGACTCTCTA CAAGGCACCC AACTCTCAGC TGGTTGCGCA TGAGTTCATT 
TTGGATATAC GCGACCTGAA GAAATCGGCC AATATCGAGG CAGTGGATGT GGCCAAGCGA 
CTCATGGATT ACGGCTTCCA TGCACCAACT ATGTCTTGGC CCGTCGCTGG AACCCTGATG 
ATCGAGCCCA CTGAATCGGA GGATAAGGAG GAGTTGGATA GGTTCTGTGA TGCCATGATT 
TCCATTCGAG AGGAGATTGC CGAGATCGAG GCGGGTCGCA TGGACAAGGC AGTCAATCCA 
CTGAAGATGT CTCCTCACAC CCAAGCTCAG GTGATCTCGG ATAAGTGGGA TCGACCATAT 
ACACGGGAGC AGGCTGCCTT CCCCGCCATC TTTGTAAAAC CAGATGCCAA GATCTGGCCC 
ACTGTGGGCA GGATCGATGA TGCCTATGGT GACAAGCACC TTGTGTGTAC CTGTCCGCCA 
ATACTGCCTG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649989.1
CDS	63..3020
Translation	MQRFLGRSSQSLLLRCGHRYLATTPVEEVIFPTKSDFPSRHIGPRKTDVVAMLDTLGFKSLAELTEKAVPQSIQLKRELDLDKPLNEHELIRRIRDISLKNQLWRSYIGMGYHNCHVPHTIIRNMFENPGWTTQYTPYQPEIAQGRLESLLNYQTLVTDLTGLDVANASLLDEGTAAAEAMCLATRHNKRKKLYLSNRVHPQTLSVVKTRTEALELEIVVGPIEQADLPSRELAGILLQYPDTYGDVKDFEDIAALAKKNGTLVVVATDLLSLTLLRPPAEFGADIAVGTSQRLGVPLGYGGPHAGFFACKQSLVRLMPGRMIGVTRDMDGNDAYRLALQTREQHIRRDKATSNICTAQALLANMSAMYAIYHGPEGLKAMANRIHHFTLTLQTGVLGAGHEVINKNFFDTLHIRLGGGQSLEDLKERAEHKRINLRYLEDDTVGVALDETVSVADVDDLLWVFKAEASVEHILARKDVLKNSIENSKFLRTSPYLQHPIFQSYHSESRMVRYMKKLENKDISLVHSMIPLGSCTMKLNSTTEMMPCSFRHFTDIHPFAPVEQAQGFHQMFKELEHDLCEITGYDRISFQPNSGAQGEYAGLRAIRSYHEHRNEGHRNICLIPISAHGTNPASAQMAGMKVEPIRILSNGSIDMAHLRAKAEEHAHELSCLMITYPSTMGVFEETVADICTLIHKHGGQVYLDGANMNAQVGLCRPGDYGSDVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPYLPGHPVVSPLSSEEHSFGVVSAAPFGSSAILPISWSYIKLMGSRGLKRATQVAILNANYMSKRLEQHYKTLYKAPNSQLVAHEFILDIRDLKKSANIEAVDVAKRLMDYGFHAPTMSWPVAGTLMIEPTESEDKEELDRFCDAMISIREEIAEIEAGRMDKAVNPLKMSPHTQAQVISDKWDRPYTREQAAFPAIFVKPDAKIWPTVGRIDDAYGDKHLVCTCPPILPDL

Target ORF information:

RefSeq Version	NM_141732.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG3999), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141732.2

ORF Insert Sequence:

ATGCAGCGAT TTTTGGGAAG GAGCAGCCAA AGCCTTTTGC TAAGATGTGG TCATCGCTAT 
CTGGCCACCA CTCCTGTGGA GGAGGTCATA TTTCCCACCA AATCGGATTT TCCCAGTCGT 
CATATTGGGC CTCGAAAGAC GGATGTGGTG GCCATGTTGG ATACTTTGGG CTTCAAGTCC 
CTGGCGGAGC TCACAGAGAA AGCTGTGCCC CAGAGCATTC AACTGAAGAG GGAACTCGAC 
CTGGACAAAC CTCTGAATGA GCACGAGCTA ATCCGCCGAA TTCGCGACAT CTCGCTAAAG 
AATCAACTGT GGCGCTCCTA CATCGGAATG GGTTACCACA ACTGCCATGT GCCGCACACC 
ATTATCCGGA ATATGTTCGA GAATCCGGGT TGGACCACTC AGTACACCCC GTACCAGCCG 
GAAATCGCCC AGGGACGCTT GGAGTCGCTG CTGAACTATC AGACCCTGGT AACCGATCTC 
ACCGGCTTGG ATGTGGCCAA TGCCTCGCTA TTGGACGAGG GAACCGCTGC TGCCGAGGCC 
ATGTGCCTGG CCACCAGGCA CAACAAGCGC AAAAAGCTCT ACCTGTCGAA CAGGGTGCAT 
CCCCAGACGC TGTCCGTAGT CAAGACGCGA ACGGAAGCCT TGGAGCTGGA AATCGTGGTG 
GGACCTATTG AACAGGCGGA TCTGCCAAGC CGCGAATTGG CTGGCATTCT TCTCCAGTAT 
CCAGATACTT ATGGTGATGT CAAGGACTTC GAGGATATCG CAGCTTTGGC AAAGAAAAAT 
GGGACCCTCG TGGTGGTGGC GACCGATTTG TTGTCGTTAA CCCTTCTGCG TCCTCCGGCG 
GAATTTGGAG CTGATATTGC AGTGGGAACC TCCCAGCGAT TGGGAGTTCC TCTGGGATAC 
GGTGGCCCCC ATGCCGGATT TTTTGCCTGC AAGCAGAGCT TGGTGCGGCT AATGCCGGGA 
AGGATGATTG GCGTGACCAG GGATATGGAC GGAAACGATG CCTATCGTCT GGCCCTGCAA 
ACCAGGGAGC AGCATATCCG GCGGGATAAG GCAACAAGTA ACATTTGCAC GGCACAGGCT 
CTTCTGGCCA ACATGTCGGC CATGTACGCC ATCTACCACG GTCCGGAGGG TCTGAAAGCC 
ATGGCCAATC GCATCCACCA TTTCACGTTG ACCCTTCAAA CGGGTGTCCT GGGAGCGGGT 
CACGAAGTGA TCAACAAGAA CTTCTTCGAC ACACTTCACA TTAGACTGGG TGGAGGTCAA 
TCCCTTGAGG ATCTCAAAGA GCGTGCCGAG CACAAGCGCA TTAATCTCCG ATATCTTGAA 
GATGACACTG TTGGCGTGGC CCTTGATGAA ACGGTCAGTG TAGCCGATGT GGACGATCTG 
TTGTGGGTCT TCAAGGCAGA GGCATCTGTG GAACACATTC TGGCGCGTAA AGATGTCCTT 
AAGAACTCGA TTGAGAACTC CAAGTTCCTG CGCACTTCGC CCTATCTGCA GCACCCTATT 
TTCCAGAGCT ATCACAGCGA GTCCCGCATG GTGCGCTACA TGAAAAAGCT GGAGAATAAG 
GACATCTCCC TGGTGCACTC CATGATCCCC CTGGGATCCT GCACTATGAA GCTGAACTCC 
ACCACCGAGA TGATGCCTTG TTCCTTCCGC CACTTCACCG ATATCCATCC TTTTGCGCCG 
GTGGAACAAG CCCAGGGATT CCATCAGATG TTCAAGGAAC TGGAGCATGA CCTATGCGAG 
ATTACAGGAT ACGATCGTAT CTCCTTCCAG CCGAACTCGG GGGCCCAAGG AGAATATGCC 
GGACTGAGAG CCATCAGGAG CTACCATGAA CACCGAAATG AAGGACACAG AAATATTTGT 
CTGATTCCAA TTTCTGCCCA CGGCACAAAT CCAGCTTCTG CACAAATGGC GGGTATGAAG 
GTGGAGCCTA TTAGAATTTT GTCGAATGGT TCCATCGACA TGGCACACCT AAGAGCTAAG 
GCAGAGGAGC ACGCCCACGA GTTGTCCTGC CTAATGATTA CCTATCCATC GACAATGGGC 
GTGTTCGAGG AAACGGTTGC CGATATTTGC ACCCTGATCC ACAAGCATGG CGGACAGGTG 
TACCTGGATG GAGCCAACAT GAATGCCCAG GTTGGGCTGT GTCGACCTGG AGATTATGGC 
AGCGATGTTT CGCATCTCAA CCTGCACAAG ACCTTCTGCA TTCCGCATGG TGGTGGTGGT 
CCTGGAATGG GTCCCATCGG CGTGAAAGCC CATTTGGCAC CATACCTTCC TGGTCATCCT 
GTTGTCAGTC CGTTGAGCAG CGAGGAGCAC AGTTTTGGAG TCGTATCCGC CGCTCCCTTC 
GGAAGCTCTG CCATTTTGCC CATATCCTGG TCGTACATTA AACTCATGGG CAGCAGGGGT 
TTGAAGAGAG CTACCCAGGT GGCCATTCTC AATGCAAACT ACATGTCCAA GAGGCTGGAG 
CAGCATTACA AGACTCTCTA CAAGGCACCC AACTCTCAGC TGGTTGCGCA TGAGTTCATT 
TTGGATATAC GCGACCTGAA GAAATCGGCC AATATCGAGG CAGTGGATGT GGCCAAGCGA 
CTCATGGATT ACGGCTTCCA TGCACCAACT ATGTCTTGGC CCGTCGCTGG AACCCTGATG 
ATCGAGCCCA CTGAATCGGA GGATAAGGAG GAGTTGGATA GGTTCTGTGA TGCCATGATT 
TCCATTCGAG AGGAGATTGC CGAGATCGAG GCGGGTCGCA TGGACAAGGC AGTCAATCCA 
CTGAAGATGT CTCCTCACAC CCAAGCTCAG GTGATCTCGG ATAAGTGGGA TCGACCATAT 
ACACGGGAGC AGGCTGCCTT CCCCGCCATC TTTGTAAAAC CAGATGCCAA GATCTGGCCC 
ACTGTGGGCA GGATCGATGA TGCCTATGGT GACAAGCACC TTGTGTGTAC CTGTCCGCCA 
ATACTGCCTG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG3999 ( NM_001275527.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262456.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG3999), mRNA.

Price & Availability↑

CloneID	OFa04445
Clone ID Related Accession (Same CDS sequence)	NM_141732.2 , NM_001275527.1
Accession Version	NM_001275527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCGAT TTTTGGGAAG GAGCAGCCAA AGCCTTTTGC TAAGATGTGG TCATCGCTAT 
CTGGCCACCA CTCCTGTGGA GGAGGTCATA TTTCCCACCA AATCGGATTT TCCCAGTCGT 
CATATTGGGC CTCGAAAGAC GGATGTGGTG GCCATGTTGG ATACTTTGGG CTTCAAGTCC 
CTGGCGGAGC TCACAGAGAA AGCTGTGCCC CAGAGCATTC AACTGAAGAG GGAACTCGAC 
CTGGACAAAC CTCTGAATGA GCACGAGCTA ATCCGCCGAA TTCGCGACAT CTCGCTAAAG 
AATCAACTGT GGCGCTCCTA CATCGGAATG GGTTACCACA ACTGCCATGT GCCGCACACC 
ATTATCCGGA ATATGTTCGA GAATCCGGGT TGGACCACTC AGTACACCCC GTACCAGCCG 
GAAATCGCCC AGGGACGCTT GGAGTCGCTG CTGAACTATC AGACCCTGGT AACCGATCTC 
ACCGGCTTGG ATGTGGCCAA TGCCTCGCTA TTGGACGAGG GAACCGCTGC TGCCGAGGCC 
ATGTGCCTGG CCACCAGGCA CAACAAGCGC AAAAAGCTCT ACCTGTCGAA CAGGGTGCAT 
CCCCAGACGC TGTCCGTAGT CAAGACGCGA ACGGAAGCCT TGGAGCTGGA AATCGTGGTG 
GGACCTATTG AACAGGCGGA TCTGCCAAGC CGCGAATTGG CTGGCATTCT TCTCCAGTAT 
CCAGATACTT ATGGTGATGT CAAGGACTTC GAGGATATCG CAGCTTTGGC AAAGAAAAAT 
GGGACCCTCG TGGTGGTGGC GACCGATTTG TTGTCGTTAA CCCTTCTGCG TCCTCCGGCG 
GAATTTGGAG CTGATATTGC AGTGGGAACC TCCCAGCGAT TGGGAGTTCC TCTGGGATAC 
GGTGGCCCCC ATGCCGGATT TTTTGCCTGC AAGCAGAGCT TGGTGCGGCT AATGCCGGGA 
AGGATGATTG GCGTGACCAG GGATATGGAC GGAAACGATG CCTATCGTCT GGCCCTGCAA 
ACCAGGGAGC AGCATATCCG GCGGGATAAG GCAACAAGTA ACATTTGCAC GGCACAGGCT 
CTTCTGGCCA ACATGTCGGC CATGTACGCC ATCTACCACG GTCCGGAGGG TCTGAAAGCC 
ATGGCCAATC GCATCCACCA TTTCACGTTG ACCCTTCAAA CGGGTGTCCT GGGAGCGGGT 
CACGAAGTGA TCAACAAGAA CTTCTTCGAC ACACTTCACA TTAGACTGGG TGGAGGTCAA 
TCCCTTGAGG ATCTCAAAGA GCGTGCCGAG CACAAGCGCA TTAATCTCCG ATATCTTGAA 
GATGACACTG TTGGCGTGGC CCTTGATGAA ACGGTCAGTG TAGCCGATGT GGACGATCTG 
TTGTGGGTCT TCAAGGCAGA GGCATCTGTG GAACACATTC TGGCGCGTAA AGATGTCCTT 
AAGAACTCGA TTGAGAACTC CAAGTTCCTG CGCACTTCGC CCTATCTGCA GCACCCTATT 
TTCCAGAGCT ATCACAGCGA GTCCCGCATG GTGCGCTACA TGAAAAAGCT GGAGAATAAG 
GACATCTCCC TGGTGCACTC CATGATCCCC CTGGGATCCT GCACTATGAA GCTGAACTCC 
ACCACCGAGA TGATGCCTTG TTCCTTCCGC CACTTCACCG ATATCCATCC TTTTGCGCCG 
GTGGAACAAG CCCAGGGATT CCATCAGATG TTCAAGGAAC TGGAGCATGA CCTATGCGAG 
ATTACAGGAT ACGATCGTAT CTCCTTCCAG CCGAACTCGG GGGCCCAAGG AGAATATGCC 
GGACTGAGAG CCATCAGGAG CTACCATGAA CACCGAAATG AAGGACACAG AAATATTTGT 
CTGATTCCAA TTTCTGCCCA CGGCACAAAT CCAGCTTCTG CACAAATGGC GGGTATGAAG 
GTGGAGCCTA TTAGAATTTT GTCGAATGGT TCCATCGACA TGGCACACCT AAGAGCTAAG 
GCAGAGGAGC ACGCCCACGA GTTGTCCTGC CTAATGATTA CCTATCCATC GACAATGGGC 
GTGTTCGAGG AAACGGTTGC CGATATTTGC ACCCTGATCC ACAAGCATGG CGGACAGGTG 
TACCTGGATG GAGCCAACAT GAATGCCCAG GTTGGGCTGT GTCGACCTGG AGATTATGGC 
AGCGATGTTT CGCATCTCAA CCTGCACAAG ACCTTCTGCA TTCCGCATGG TGGTGGTGGT 
CCTGGAATGG GTCCCATCGG CGTGAAAGCC CATTTGGCAC CATACCTTCC TGGTCATCCT 
GTTGTCAGTC CGTTGAGCAG CGAGGAGCAC AGTTTTGGAG TCGTATCCGC CGCTCCCTTC 
GGAAGCTCTG CCATTTTGCC CATATCCTGG TCGTACATTA AACTCATGGG CAGCAGGGGT 
TTGAAGAGAG CTACCCAGGT GGCCATTCTC AATGCAAACT ACATGTCCAA GAGGCTGGAG 
CAGCATTACA AGACTCTCTA CAAGGCACCC AACTCTCAGC TGGTTGCGCA TGAGTTCATT 
TTGGATATAC GCGACCTGAA GAAATCGGCC AATATCGAGG CAGTGGATGT GGCCAAGCGA 
CTCATGGATT ACGGCTTCCA TGCACCAACT ATGTCTTGGC CCGTCGCTGG AACCCTGATG 
ATCGAGCCCA CTGAATCGGA GGATAAGGAG GAGTTGGATA GGTTCTGTGA TGCCATGATT 
TCCATTCGAG AGGAGATTGC CGAGATCGAG GCGGGTCGCA TGGACAAGGC AGTCAATCCA 
CTGAAGATGT CTCCTCACAC CCAAGCTCAG GTGATCTCGG ATAAGTGGGA TCGACCATAT 
ACACGGGAGC AGGCTGCCTT CCCCGCCATC TTTGTAAAAC CAGATGCCAA GATCTGGCCC 
ACTGTGGGCA GGATCGATGA TGCCTATGGT GACAAGCACC TTGTGTGTAC CTGTCCGCCA 
ATACTGCCTG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262456.1
CDS	63..3020
Translation	MQRFLGRSSQSLLLRCGHRYLATTPVEEVIFPTKSDFPSRHIGPRKTDVVAMLDTLGFKSLAELTEKAVPQSIQLKRELDLDKPLNEHELIRRIRDISLKNQLWRSYIGMGYHNCHVPHTIIRNMFENPGWTTQYTPYQPEIAQGRLESLLNYQTLVTDLTGLDVANASLLDEGTAAAEAMCLATRHNKRKKLYLSNRVHPQTLSVVKTRTEALELEIVVGPIEQADLPSRELAGILLQYPDTYGDVKDFEDIAALAKKNGTLVVVATDLLSLTLLRPPAEFGADIAVGTSQRLGVPLGYGGPHAGFFACKQSLVRLMPGRMIGVTRDMDGNDAYRLALQTREQHIRRDKATSNICTAQALLANMSAMYAIYHGPEGLKAMANRIHHFTLTLQTGVLGAGHEVINKNFFDTLHIRLGGGQSLEDLKERAEHKRINLRYLEDDTVGVALDETVSVADVDDLLWVFKAEASVEHILARKDVLKNSIENSKFLRTSPYLQHPIFQSYHSESRMVRYMKKLENKDISLVHSMIPLGSCTMKLNSTTEMMPCSFRHFTDIHPFAPVEQAQGFHQMFKELEHDLCEITGYDRISFQPNSGAQGEYAGLRAIRSYHEHRNEGHRNICLIPISAHGTNPASAQMAGMKVEPIRILSNGSIDMAHLRAKAEEHAHELSCLMITYPSTMGVFEETVADICTLIHKHGGQVYLDGANMNAQVGLCRPGDYGSDVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPYLPGHPVVSPLSSEEHSFGVVSAAPFGSSAILPISWSYIKLMGSRGLKRATQVAILNANYMSKRLEQHYKTLYKAPNSQLVAHEFILDIRDLKKSANIEAVDVAKRLMDYGFHAPTMSWPVAGTLMIEPTESEDKEELDRFCDAMISIREEIAEIEAGRMDKAVNPLKMSPHTQAQVISDKWDRPYTREQAAFPAIFVKPDAKIWPTVGRIDDAYGDKHLVCTCPPILPDL

Target ORF information:

RefSeq Version	NM_001275527.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG3999), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275527.1

ORF Insert Sequence:

ATGCAGCGAT TTTTGGGAAG GAGCAGCCAA AGCCTTTTGC TAAGATGTGG TCATCGCTAT 
CTGGCCACCA CTCCTGTGGA GGAGGTCATA TTTCCCACCA AATCGGATTT TCCCAGTCGT 
CATATTGGGC CTCGAAAGAC GGATGTGGTG GCCATGTTGG ATACTTTGGG CTTCAAGTCC 
CTGGCGGAGC TCACAGAGAA AGCTGTGCCC CAGAGCATTC AACTGAAGAG GGAACTCGAC 
CTGGACAAAC CTCTGAATGA GCACGAGCTA ATCCGCCGAA TTCGCGACAT CTCGCTAAAG 
AATCAACTGT GGCGCTCCTA CATCGGAATG GGTTACCACA ACTGCCATGT GCCGCACACC 
ATTATCCGGA ATATGTTCGA GAATCCGGGT TGGACCACTC AGTACACCCC GTACCAGCCG 
GAAATCGCCC AGGGACGCTT GGAGTCGCTG CTGAACTATC AGACCCTGGT AACCGATCTC 
ACCGGCTTGG ATGTGGCCAA TGCCTCGCTA TTGGACGAGG GAACCGCTGC TGCCGAGGCC 
ATGTGCCTGG CCACCAGGCA CAACAAGCGC AAAAAGCTCT ACCTGTCGAA CAGGGTGCAT 
CCCCAGACGC TGTCCGTAGT CAAGACGCGA ACGGAAGCCT TGGAGCTGGA AATCGTGGTG 
GGACCTATTG AACAGGCGGA TCTGCCAAGC CGCGAATTGG CTGGCATTCT TCTCCAGTAT 
CCAGATACTT ATGGTGATGT CAAGGACTTC GAGGATATCG CAGCTTTGGC AAAGAAAAAT 
GGGACCCTCG TGGTGGTGGC GACCGATTTG TTGTCGTTAA CCCTTCTGCG TCCTCCGGCG 
GAATTTGGAG CTGATATTGC AGTGGGAACC TCCCAGCGAT TGGGAGTTCC TCTGGGATAC 
GGTGGCCCCC ATGCCGGATT TTTTGCCTGC AAGCAGAGCT TGGTGCGGCT AATGCCGGGA 
AGGATGATTG GCGTGACCAG GGATATGGAC GGAAACGATG CCTATCGTCT GGCCCTGCAA 
ACCAGGGAGC AGCATATCCG GCGGGATAAG GCAACAAGTA ACATTTGCAC GGCACAGGCT 
CTTCTGGCCA ACATGTCGGC CATGTACGCC ATCTACCACG GTCCGGAGGG TCTGAAAGCC 
ATGGCCAATC GCATCCACCA TTTCACGTTG ACCCTTCAAA CGGGTGTCCT GGGAGCGGGT 
CACGAAGTGA TCAACAAGAA CTTCTTCGAC ACACTTCACA TTAGACTGGG TGGAGGTCAA 
TCCCTTGAGG ATCTCAAAGA GCGTGCCGAG CACAAGCGCA TTAATCTCCG ATATCTTGAA 
GATGACACTG TTGGCGTGGC CCTTGATGAA ACGGTCAGTG TAGCCGATGT GGACGATCTG 
TTGTGGGTCT TCAAGGCAGA GGCATCTGTG GAACACATTC TGGCGCGTAA AGATGTCCTT 
AAGAACTCGA TTGAGAACTC CAAGTTCCTG CGCACTTCGC CCTATCTGCA GCACCCTATT 
TTCCAGAGCT ATCACAGCGA GTCCCGCATG GTGCGCTACA TGAAAAAGCT GGAGAATAAG 
GACATCTCCC TGGTGCACTC CATGATCCCC CTGGGATCCT GCACTATGAA GCTGAACTCC 
ACCACCGAGA TGATGCCTTG TTCCTTCCGC CACTTCACCG ATATCCATCC TTTTGCGCCG 
GTGGAACAAG CCCAGGGATT CCATCAGATG TTCAAGGAAC TGGAGCATGA CCTATGCGAG 
ATTACAGGAT ACGATCGTAT CTCCTTCCAG CCGAACTCGG GGGCCCAAGG AGAATATGCC 
GGACTGAGAG CCATCAGGAG CTACCATGAA CACCGAAATG AAGGACACAG AAATATTTGT 
CTGATTCCAA TTTCTGCCCA CGGCACAAAT CCAGCTTCTG CACAAATGGC GGGTATGAAG 
GTGGAGCCTA TTAGAATTTT GTCGAATGGT TCCATCGACA TGGCACACCT AAGAGCTAAG 
GCAGAGGAGC ACGCCCACGA GTTGTCCTGC CTAATGATTA CCTATCCATC GACAATGGGC 
GTGTTCGAGG AAACGGTTGC CGATATTTGC ACCCTGATCC ACAAGCATGG CGGACAGGTG 
TACCTGGATG GAGCCAACAT GAATGCCCAG GTTGGGCTGT GTCGACCTGG AGATTATGGC 
AGCGATGTTT CGCATCTCAA CCTGCACAAG ACCTTCTGCA TTCCGCATGG TGGTGGTGGT 
CCTGGAATGG GTCCCATCGG CGTGAAAGCC CATTTGGCAC CATACCTTCC TGGTCATCCT 
GTTGTCAGTC CGTTGAGCAG CGAGGAGCAC AGTTTTGGAG TCGTATCCGC CGCTCCCTTC 
GGAAGCTCTG CCATTTTGCC CATATCCTGG TCGTACATTA AACTCATGGG CAGCAGGGGT 
TTGAAGAGAG CTACCCAGGT GGCCATTCTC AATGCAAACT ACATGTCCAA GAGGCTGGAG 
CAGCATTACA AGACTCTCTA CAAGGCACCC AACTCTCAGC TGGTTGCGCA TGAGTTCATT 
TTGGATATAC GCGACCTGAA GAAATCGGCC AATATCGAGG CAGTGGATGT GGCCAAGCGA 
CTCATGGATT ACGGCTTCCA TGCACCAACT ATGTCTTGGC CCGTCGCTGG AACCCTGATG 
ATCGAGCCCA CTGAATCGGA GGATAAGGAG GAGTTGGATA GGTTCTGTGA TGCCATGATT 
TCCATTCGAG AGGAGATTGC CGAGATCGAG GCGGGTCGCA TGGACAAGGC AGTCAATCCA 
CTGAAGATGT CTCCTCACAC CCAAGCTCAG GTGATCTCGG ATAAGTGGGA TCGACCATAT 
ACACGGGAGC AGGCTGCCTT CCCCGCCATC TTTGTAAAAC CAGATGCCAA GATCTGGCCC 
ACTGTGGGCA GGATCGATGA TGCCTATGGT GACAAGCACC TTGTGTGTAC CTGTCCGCCA 
ATACTGCCTG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG3999 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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