Spn85F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spn85F
Entrez Gene ID	41221
Full Name	Serpin 85F
Synonyms	CG12807,Dmel\CG12807
General protein information	Preferred Names Serpin 85F Names CG12807 gene product from transcript CG12807-RA CG12807-PA Spn85F-PA serpin 85F
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85F5-85F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_141708.3	NP_649965.2	serpin 85F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spn85F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spn85F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04415	NM_141708.3 Latest version!	Drosophila melanogaster serpin 85F (Spn85F), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spn85F ( NM_141708.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649965.2 Drosophila melanogaster serpin 85F (Spn85F), partial mRNA.

Price & Availability↑

CloneID	OFa04415
Clone ID Related Accession (Same CDS sequence)	NM_141708.3
Accession Version	NM_141708.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serpin 85F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141708.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 3' end.

ATGGCTGGAT TGGTGCTACT GTTCACCTGG CTGTTGGTCC ACCTACTCCA GGTGGATGCC 
CACTATATAC AAATTGACCA TGAAGAATTT CGTGCCAGCG GCTTTCCGCA TCCGCACTTT 
CCGCCGCCTC CGCTAACGCC GCTGGATCAT CTACCTCAGG TGCTGGCCGC CAAGGAGCTG 
ACTCCCGCCG AGGTGATGGT GGACCTGACC AACGACCTGA CCCACCGCCT GCTCCACTAC 
CATTCCATCC TGAACCGCAA CAACTTCGCC TTCTCGCCCA CTGCCTTGGT CAGTGTGCTG 
GTGGCTCTGT TCGAGGGTTC GGCCGGGAAT ACCGCCGAGG AACTGCGCCA TGTCCTGCAG 
CTGCCCAACA ACAGGGATGT GACCCGCGTG GGCTACCGCG ACATCCATCG ACGACTGCGG 
ACTTACTTTT TTGGCTCCGA CAATCCACTG AAAGGACTCA GCTTGAACAA GGGCAATGTC 
ACTGTTCTCA AGGACTTTGA GACGGTTCTT ATGTTTTACG GATATGACTT AAGTGTAGAT 
ATGCTCTCCT CCACACCAGC CAATCTCACA TCAGATGCCG AGTTGGTCAA CACCACAATG 
GCCAGCAATA CGACGACCAT GGAAATGGAT GCGGAAACCA CAACGTCAAA GGATGCGGAG 
AGCACAACAG AAGAGCCGGC CACAACAACC ACAGAGCCGC CGGCGACGAC AACGGCAGAG 
CCACCAACCA CCACCACAGA GACTCCAGCG GAAACGGAGG CCACCACAGA AGCACCTGCT 
GCAACATCGG GTGAAGAGGA GGCCGCTGAG CCCGCTGGTG AGGATGAAGC AGCCGAGTTA 
GTGTCCGCTT TTGTAGCCGA GGAGGACTCC GAGCCTCTCT TGCGCATCCA GAAGGCTCGA 
GTCTCCAGAT TGCCCACCAG CAAACTACAG GCGCCGCTAA AGCATTCGAA TCCAATCACT 
CCCAAGCCCA TTTACCTGCC CAGTGCGCGA ACAGTGGCCA TGCCAATGCC AATGATGGCT 
CGCCAGGTGG CGGAGCGAAA GCCACCAGGA ACACGGCAAG TAATTTCTAT TATAGCGCCA 
TCCGTGCAAC CAGCAAACAT ATCGGTGACT CGCAAGACGA AGACAGCACG ATACAAGCGT 
CATGCGATAC CAGCATACAA GGATCTGGAT GCAAATCTCT TTCTGACCCT CTTCAATCCG 
CACACGCATG TCCCGCACCA CCCGCTGCAC TTTCCGCCGC CAGTGCCCGC TGCCTTTGCA 
CCCATCACGA ATGATTTTGA GCCACACTAT ATCGGAGAGG CGGCCGAGGG AAAGTCCAAC 
TATAACACGG ATGTGATCAG CCACGTATTC TACTTGGGCA ATCAACAGGT GGTGCACACC 
ACCTTCAAGG TATATAACGC AGTGCTGTAC TACAAGTACT TTGAGCACCT GAAGATGAGC 
GTGCTGGAGT TGGAATTGGA CACACCGGAG TACAACCTAA TGATCCTGTT GCCCGACTAC 
CACACGGATA TTGTTGCAGC CGCCGCCTCC TTGAAACTGG GACCAACCCT TCGACTGATG 
CGGAAGCAGT TGAAGCCACG CTGGGTGCAA GCCATCATTC CAGATTTCAA GCTGCACGGC 
ACCATGTTTT TGACAAACGA CTTGCAGAAT ATGGGAATCT GCGACGTATT CGAACCAAAT 
CGCGCCGATT TCAGGCCCAT GACTGAGGAG AAGGGCGTCT ACGTGCGACA CATTGAGCAA 
TCCATAGACG TCACCATCCG TACGCATCCC ATCAATCAGC TGAAGCGCAA CTACGGCGCC 
CAATCGAAGC CCATTCAGAT CTCTGTGAAT CATCCGTTTC TGTTTTTCAT CGTCGATCGC 
GACTTGGACG TGGCTGTGAT GTCGGGCCGC ATACTGAATC CGCTGAACGT ACGCATCCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649965.2
CDS	377..2299
Translation	MAGLVLLFTWLLVHLLQVDAHYIQIDHEEFRASGFPHPHFPPPPLTPLDHLPQVLAAKELTPAEVMVDLTNDLTHRLLHYHSILNRNNFAFSPTALVSVLVALFEGSAGNTAEELRHVLQLPNNRDVTRVGYRDIHRRLRTYFFGSDNPLKGLSLNKGNVTVLKDFETVLMFYGYDLSVDMLSSTPANLTSDAELVNTTMASNTTTMEMDAETTTSKDAESTTEEPATTTTEPPATTTAEPPTTTTETPAETEATTEAPAATSGEEEAAEPAGEDEAAELVSAFVAEEDSEPLLRIQKARVSRLPTSKLQAPLKHSNPITPKPIYLPSARTVAMPMPMMARQVAERKPPGTRQVISIIAPSVQPANISVTRKTKTARYKRHAIPAYKDLDANLFLTLFNPHTHVPHHPLHFPPPVPAAFAPITNDFEPHYIGEAAEGKSNYNTDVISHVFYLGNQQVVHTTFKVYNAVLYYKYFEHLKMSVLELELDTPEYNLMILLPDYHTDIVAAAASLKLGPTLRLMRKQLKPRWVQAIIPDFKLHGTMFLTNDLQNMGICDVFEPNRADFRPMTEEKGVYVRHIEQSIDVTIRTHPINQLKRNYGAQSKPIQISVNHPFLFFIVDRDLDVAVMSGRILNPLNVRIQ

Target ORF information:

RefSeq Version	NM_141708.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serpin 85F (Spn85F), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141708.3

ORF Insert Sequence:

ATGGCTGGAT TGGTGCTACT GTTCACCTGG CTGTTGGTCC ACCTACTCCA GGTGGATGCC 
CACTATATAC AAATTGACCA TGAAGAATTT CGTGCCAGCG GCTTTCCGCA TCCGCACTTT 
CCGCCGCCTC CGCTAACGCC GCTGGATCAT CTACCTCAGG TGCTGGCCGC CAAGGAGCTG 
ACTCCCGCCG AGGTGATGGT GGACCTGACC AACGACCTGA CCCACCGCCT GCTCCACTAC 
CATTCCATCC TGAACCGCAA CAACTTCGCC TTCTCGCCCA CTGCCTTGGT CAGTGTGCTG 
GTGGCTCTGT TCGAGGGTTC GGCCGGGAAT ACCGCCGAGG AACTGCGCCA TGTCCTGCAG 
CTGCCCAACA ACAGGGATGT GACCCGCGTG GGCTACCGCG ACATCCATCG ACGACTGCGG 
ACTTACTTTT TTGGCTCCGA CAATCCACTG AAAGGACTCA GCTTGAACAA GGGCAATGTC 
ACTGTTCTCA AGGACTTTGA GACGGTTCTT ATGTTTTACG GATATGACTT AAGTGTAGAT 
ATGCTCTCCT CCACACCAGC CAATCTCACA TCAGATGCCG AGTTGGTCAA CACCACAATG 
GCCAGCAATA CGACGACCAT GGAAATGGAT GCGGAAACCA CAACGTCAAA GGATGCGGAG 
AGCACAACAG AAGAGCCGGC CACAACAACC ACAGAGCCGC CGGCGACGAC AACGGCAGAG 
CCACCAACCA CCACCACAGA GACTCCAGCG GAAACGGAGG CCACCACAGA AGCACCTGCT 
GCAACATCGG GTGAAGAGGA GGCCGCTGAG CCCGCTGGTG AGGATGAAGC AGCCGAGTTA 
GTGTCCGCTT TTGTAGCCGA GGAGGACTCC GAGCCTCTCT TGCGCATCCA GAAGGCTCGA 
GTCTCCAGAT TGCCCACCAG CAAACTACAG GCGCCGCTAA AGCATTCGAA TCCAATCACT 
CCCAAGCCCA TTTACCTGCC CAGTGCGCGA ACAGTGGCCA TGCCAATGCC AATGATGGCT 
CGCCAGGTGG CGGAGCGAAA GCCACCAGGA ACACGGCAAG TAATTTCTAT TATAGCGCCA 
TCCGTGCAAC CAGCAAACAT ATCGGTGACT CGCAAGACGA AGACAGCACG ATACAAGCGT 
CATGCGATAC CAGCATACAA GGATCTGGAT GCAAATCTCT TTCTGACCCT CTTCAATCCG 
CACACGCATG TCCCGCACCA CCCGCTGCAC TTTCCGCCGC CAGTGCCCGC TGCCTTTGCA 
CCCATCACGA ATGATTTTGA GCCACACTAT ATCGGAGAGG CGGCCGAGGG AAAGTCCAAC 
TATAACACGG ATGTGATCAG CCACGTATTC TACTTGGGCA ATCAACAGGT GGTGCACACC 
ACCTTCAAGG TATATAACGC AGTGCTGTAC TACAAGTACT TTGAGCACCT GAAGATGAGC 
GTGCTGGAGT TGGAATTGGA CACACCGGAG TACAACCTAA TGATCCTGTT GCCCGACTAC 
CACACGGATA TTGTTGCAGC CGCCGCCTCC TTGAAACTGG GACCAACCCT TCGACTGATG 
CGGAAGCAGT TGAAGCCACG CTGGGTGCAA GCCATCATTC CAGATTTCAA GCTGCACGGC 
ACCATGTTTT TGACAAACGA CTTGCAGAAT ATGGGAATCT GCGACGTATT CGAACCAAAT 
CGCGCCGATT TCAGGCCCAT GACTGAGGAG AAGGGCGTCT ACGTGCGACA CATTGAGCAA 
TCCATAGACG TCACCATCCG TACGCATCCC ATCAATCAGC TGAAGCGCAA CTACGGCGCC 
CAATCGAAGC CCATTCAGAT CTCTGTGAAT CATCCGTTTC TGTTTTTCAT CGTCGATCGC 
GACTTGGACG TGGCTGTGAT GTCGGGCCGC ATACTGAATC CGCTGAACGT ACGCATCCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Spn85F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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