side-VII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	side-VII
Entrez Gene ID	41184
Full Name	CG12950 gene product from transcript CG12950-RB
Synonyms	12950,CT32101,Dmel\CG12950
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85E6-85E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_141676.3	NP_649933.1	sidestep VII, isoform A
NM_001275503.1	NP_001262432.1	sidestep VII, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG12950	NP_649933.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004230	XP_313139.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following side-VII gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the side-VII cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04379	NM_001275503.1 Latest version!	Drosophila melanogaster sidestep VII (side-VII), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa04379	NM_141676.3 Latest version!	Drosophila melanogaster sidestep VII (side-VII), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

side-VII ( NM_141676.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649933.1 Drosophila melanogaster sidestep VII (side-VII), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04379
Clone ID Related Accession (Same CDS sequence)	NM_141676.3 , NM_001275503.1
Accession Version	NM_141676.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sidestep VII, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141676.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTTAC TGCTGCTGTT TATGCTAGCA CTTCAACTTA TAGAATCCAG CATCGGAAGT 
AAAGATGTAC CAATTCACCA AATTGAATCT ATAGTTGGTG AAAATATTTA TCTTCCTTGC 
AATGTAACAA CTTATGATGG TGATGAACCT GTGCTGGTTC TGTGGTACCG AGATGATAAG 
GGTACTCCAA TTTACAGCAT TGACATTCGA GCTGGAGTAT CCAAAGCACC GAAAAGATGG 
TCGGACGACT CCGTATTTGG GGATAGAGCA TATTTTATCT TTGACAAGGA ACCGGGAAAA 
CTGTCAATTC AGAATACGCA AGCATCCGAC TCCGGAACTT ATAGATGTCG CGTGGACTTC 
CTGAAAGCGC AGACCATAAA CAGCCGCATT AGGCTCAATG TCATATCTCC ACCCAAACAA 
GTTATTATTC GAGACAGCTC CAATGTTGAG CGTTCTACAG TTGTGGGACC ATATAGCGAA 
GGCGACATCG TATCCCTGAA ATGCCAAGTA ATTGGAGGCT ATCCCACGCC CACAATCAGT 
TGGTATCGCG ATGGCATAGA AATTCCATGC GAATTGTCTC ATTTGGCAGG CGGCAAAATT 
ATAGAATGCG AAATCACGCT ACCGTCTCTG GGTCGGGAGG ATCTTAACTC CCGGCTGACC 
TGTCGTGCAC TGAGTCACCC CCGGGCACCC ATCGTCGAAG CGGTGGTTCA AATCGATATG 
AATTTTGCCC CTTTAAACAT CCGACTGTTG GGGGCACATC AACCATTGTC TGCTGGACGC 
AGATACGATC TATTGTGCCA AAGTGCTGGC TCCAGGCCGC CGGCCGTAAT TACTTGGTGG 
CAAAATGGCA TACGGCTGGA GAAGACAACC GAAACGACTT CAAGCGATGG CAACCAGACC 
ACAAGCACTC TCTCAATCAG CCTAAGCAAA TCAGATGCGG GAAAGTATTT ATCATGCAAA 
GCCTACAACC ATGCCGTTCC CTCCGAGCCC CTGGAAGATG GCTGGAAATT AGATATACAA 
TATGTCCCGG AAGCGTACGT TCGTTTGGGA ACATCTCTGG ATCCGAGCAC TTTACGAGAA 
GGAACAGATG TCTACTTTGA TTGCCTGGTG ATGGCCCATC CAAATGTCTT TCGCATCGAA 
TGGCGTCACA ACGAACAACC TTTATCACAC AATATATCAC TGGGCGTCAT AATTAGTAAT 
CACTCGTTGG TACTGCAAGG TGTAACTAGA GCCACTGCGG GAAACTATTC CTGTGTGGGT 
TTCAATGCCG AAGGAGAAGG AATCAGTGCT CCCTTTGCAC TAAACATACT CTACGCCCCC 
ACGTGTGCCC AGAATCAGAA GAAGGTGTAC GGCATAGCCA AGCAGGAGGA CGCGAAGGTC 
ATGTGCACCG TGGACGCTAA TCCGCGCGAG GTCGAGTTTA GCTGGACATT TAATAATTCT 
GCCGAGAGTA TTGATGTCGC CACAAATCAT ATTATACGCT CGGGTACCAC CTCCATTGTA 
ACATACACAC CCATTACGGA ACTGGACTAC GGAACCCTTT TGTGTTTGGC GTCGAACAAA 
ATTGGCAAAC AGCGAGTGCC ATGTGTCTTC CATATAATCG CCGCAGGACG ACCGGAAAAA 
GTTCATAATT GCACGGTGAA CAATATATCT ATGACATCCT TGACGGTCAC TTGTAGTGAT 
GGTTTCAACG GCGGCTTGCC ACAGAGTTTT AATTTAGAAC TGCTGGACTC TTACACACAA 
GAAATTAAAG CTAATATAAC ATCCACTATT CCCAAGTTTA CGGTGCCAGG TTTGAGTCCG 
GGAAGTATAT ATAGGCTGAA TATTTACGCG TTCAATACGA AGGGTCGATC CGATCCAGCT 
ATAGTCAATG CGGCCATGCT GCGAATGCCC GAGAAGCAAC TGACCTCGGA ACAGACCCAC 
AATCTTCGCG CTGAGCTGCT ATTTTCACCA GTGATGTCCT TAACAATTGG CCTAACCCTC 
GCTGTGCTGG TCGCAGTTTT GGCCATCATT CTGGCACTTC GAATACCCTG TACCGCTTCA 
TCGAGACGGC GTCAAAAGGA ACTATCGAAT GAAAATATAT CCAGAGATGA GTCGCCTGGA 
CCAAGCGACA AGAGTTCCGG CAGCAAGGAA GTGGACGATT GCGATGAAAA GAATCCGGAT 
GTGGTACCCG AATCCATAGA ACCGGATGAG CAGGCCGAGA GCATCAGGAA AAGGCAGCAG 
ATATCGACAA TTGATACCAA TCGCAGTCCC AATCGAGGAA TTTTCGTGAA TGAGCAGCAG 
CATCTGACAG CAGCCGAGAT TTCCCTGCGT GCCTCGCATG GAATTGGATA CTGCACCCTG 
AGGAGTGGAA TGCATGCCCA GGCCCAGAGT TCGGTGGGCG AAATCTCAAT AAACGTAACG 
CCACACGTGT ACTCCAATTC GATCTCGCAA TGCACTCTGC CACGTCAGTC GTCGCAGAAC 
GTGTGGAACA ACTACAACTG CAACATCACC GGTGCGAGGC CGACCTCCAC GTTCCACCAG 
CTGACCTTTC CCCAGGGCAG GGTCAATGTC GGTGGTCACA ATCCGGCGCA GCCGCTCCAT 
GGACACGCAC CATCGCCGTC GCAGGCATCC AATAGCTCTA TGGCCTCGTC GACGAACACA 
TTGCCACAGC CACATCCACA TGGACGAACC ATCGCCATGC ACCACATGAA CCAGGCCCAG 
TGTGGCAGAG TCTGCATCGG AATCGCCAGC GATGGCATAC ACTCCGCCGA GCAGAATGTA 
TCCGACGACG AGGTCACCGT CCAAACTCCA TTAATGATAA AGCGCGACAG CACCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649933.1
CDS	660..3479
Translation	MELLLLFMLALQLIESSIGSKDVPIHQIESIVGENIYLPCNVTTYDGDEPVLVLWYRDDKGTPIYSIDIRAGVSKAPKRWSDDSVFGDRAYFIFDKEPGKLSIQNTQASDSGTYRCRVDFLKAQTINSRIRLNVISPPKQVIIRDSSNVERSTVVGPYSEGDIVSLKCQVIGGYPTPTISWYRDGIEIPCELSHLAGGKIIECEITLPSLGREDLNSRLTCRALSHPRAPIVEAVVQIDMNFAPLNIRLLGAHQPLSAGRRYDLLCQSAGSRPPAVITWWQNGIRLEKTTETTSSDGNQTTSTLSISLSKSDAGKYLSCKAYNHAVPSEPLEDGWKLDIQYVPEAYVRLGTSLDPSTLREGTDVYFDCLVMAHPNVFRIEWRHNEQPLSHNISLGVIISNHSLVLQGVTRATAGNYSCVGFNAEGEGISAPFALNILYAPTCAQNQKKVYGIAKQEDAKVMCTVDANPREVEFSWTFNNSAESIDVATNHIIRSGTTSIVTYTPITELDYGTLLCLASNKIGKQRVPCVFHIIAAGRPEKVHNCTVNNISMTSLTVTCSDGFNGGLPQSFNLELLDSYTQEIKANITSTIPKFTVPGLSPGSIYRLNIYAFNTKGRSDPAIVNAAMLRMPEKQLTSEQTHNLRAELLFSPVMSLTIGLTLAVLVAVLAIILALRIPCTASSRRRQKELSNENISRDESPGPSDKSSGSKEVDDCDEKNPDVVPESIEPDEQAESIRKRQQISTIDTNRSPNRGIFVNEQQHLTAAEISLRASHGIGYCTLRSGMHAQAQSSVGEISINVTPHVYSNSISQCTLPRQSSQNVWNNYNCNITGARPTSTFHQLTFPQGRVNVGGHNPAQPLHGHAPSPSQASNSSMASSTNTLPQPHPHGRTIAMHHMNQAQCGRVCIGIASDGIHSAEQNVSDDEVTVQTPLMIKRDSTV

Target ORF information:

RefSeq Version	NM_141676.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sidestep VII (side-VII), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141676.3

ORF Insert Sequence:

ATGGAGTTAC TGCTGCTGTT TATGCTAGCA CTTCAACTTA TAGAATCCAG CATCGGAAGT 
AAAGATGTAC CAATTCACCA AATTGAATCT ATAGTTGGTG AAAATATTTA TCTTCCTTGC 
AATGTAACAA CTTATGATGG TGATGAACCT GTGCTGGTTC TGTGGTACCG AGATGATAAG 
GGTACTCCAA TTTACAGCAT TGACATTCGA GCTGGAGTAT CCAAAGCACC GAAAAGATGG 
TCGGACGACT CCGTATTTGG GGATAGAGCA TATTTTATCT TTGACAAGGA ACCGGGAAAA 
CTGTCAATTC AGAATACGCA AGCATCCGAC TCCGGAACTT ATAGATGTCG CGTGGACTTC 
CTGAAAGCGC AGACCATAAA CAGCCGCATT AGGCTCAATG TCATATCTCC ACCCAAACAA 
GTTATTATTC GAGACAGCTC CAATGTTGAG CGTTCTACAG TTGTGGGACC ATATAGCGAA 
GGCGACATCG TATCCCTGAA ATGCCAAGTA ATTGGAGGCT ATCCCACGCC CACAATCAGT 
TGGTATCGCG ATGGCATAGA AATTCCATGC GAATTGTCTC ATTTGGCAGG CGGCAAAATT 
ATAGAATGCG AAATCACGCT ACCGTCTCTG GGTCGGGAGG ATCTTAACTC CCGGCTGACC 
TGTCGTGCAC TGAGTCACCC CCGGGCACCC ATCGTCGAAG CGGTGGTTCA AATCGATATG 
AATTTTGCCC CTTTAAACAT CCGACTGTTG GGGGCACATC AACCATTGTC TGCTGGACGC 
AGATACGATC TATTGTGCCA AAGTGCTGGC TCCAGGCCGC CGGCCGTAAT TACTTGGTGG 
CAAAATGGCA TACGGCTGGA GAAGACAACC GAAACGACTT CAAGCGATGG CAACCAGACC 
ACAAGCACTC TCTCAATCAG CCTAAGCAAA TCAGATGCGG GAAAGTATTT ATCATGCAAA 
GCCTACAACC ATGCCGTTCC CTCCGAGCCC CTGGAAGATG GCTGGAAATT AGATATACAA 
TATGTCCCGG AAGCGTACGT TCGTTTGGGA ACATCTCTGG ATCCGAGCAC TTTACGAGAA 
GGAACAGATG TCTACTTTGA TTGCCTGGTG ATGGCCCATC CAAATGTCTT TCGCATCGAA 
TGGCGTCACA ACGAACAACC TTTATCACAC AATATATCAC TGGGCGTCAT AATTAGTAAT 
CACTCGTTGG TACTGCAAGG TGTAACTAGA GCCACTGCGG GAAACTATTC CTGTGTGGGT 
TTCAATGCCG AAGGAGAAGG AATCAGTGCT CCCTTTGCAC TAAACATACT CTACGCCCCC 
ACGTGTGCCC AGAATCAGAA GAAGGTGTAC GGCATAGCCA AGCAGGAGGA CGCGAAGGTC 
ATGTGCACCG TGGACGCTAA TCCGCGCGAG GTCGAGTTTA GCTGGACATT TAATAATTCT 
GCCGAGAGTA TTGATGTCGC CACAAATCAT ATTATACGCT CGGGTACCAC CTCCATTGTA 
ACATACACAC CCATTACGGA ACTGGACTAC GGAACCCTTT TGTGTTTGGC GTCGAACAAA 
ATTGGCAAAC AGCGAGTGCC ATGTGTCTTC CATATAATCG CCGCAGGACG ACCGGAAAAA 
GTTCATAATT GCACGGTGAA CAATATATCT ATGACATCCT TGACGGTCAC TTGTAGTGAT 
GGTTTCAACG GCGGCTTGCC ACAGAGTTTT AATTTAGAAC TGCTGGACTC TTACACACAA 
GAAATTAAAG CTAATATAAC ATCCACTATT CCCAAGTTTA CGGTGCCAGG TTTGAGTCCG 
GGAAGTATAT ATAGGCTGAA TATTTACGCG TTCAATACGA AGGGTCGATC CGATCCAGCT 
ATAGTCAATG CGGCCATGCT GCGAATGCCC GAGAAGCAAC TGACCTCGGA ACAGACCCAC 
AATCTTCGCG CTGAGCTGCT ATTTTCACCA GTGATGTCCT TAACAATTGG CCTAACCCTC 
GCTGTGCTGG TCGCAGTTTT GGCCATCATT CTGGCACTTC GAATACCCTG TACCGCTTCA 
TCGAGACGGC GTCAAAAGGA ACTATCGAAT GAAAATATAT CCAGAGATGA GTCGCCTGGA 
CCAAGCGACA AGAGTTCCGG CAGCAAGGAA GTGGACGATT GCGATGAAAA GAATCCGGAT 
GTGGTACCCG AATCCATAGA ACCGGATGAG CAGGCCGAGA GCATCAGGAA AAGGCAGCAG 
ATATCGACAA TTGATACCAA TCGCAGTCCC AATCGAGGAA TTTTCGTGAA TGAGCAGCAG 
CATCTGACAG CAGCCGAGAT TTCCCTGCGT GCCTCGCATG GAATTGGATA CTGCACCCTG 
AGGAGTGGAA TGCATGCCCA GGCCCAGAGT TCGGTGGGCG AAATCTCAAT AAACGTAACG 
CCACACGTGT ACTCCAATTC GATCTCGCAA TGCACTCTGC CACGTCAGTC GTCGCAGAAC 
GTGTGGAACA ACTACAACTG CAACATCACC GGTGCGAGGC CGACCTCCAC GTTCCACCAG 
CTGACCTTTC CCCAGGGCAG GGTCAATGTC GGTGGTCACA ATCCGGCGCA GCCGCTCCAT 
GGACACGCAC CATCGCCGTC GCAGGCATCC AATAGCTCTA TGGCCTCGTC GACGAACACA 
TTGCCACAGC CACATCCACA TGGACGAACC ATCGCCATGC ACCACATGAA CCAGGCCCAG 
TGTGGCAGAG TCTGCATCGG AATCGCCAGC GATGGCATAC ACTCCGCCGA GCAGAATGTA 
TCCGACGACG AGGTCACCGT CCAAACTCCA TTAATGATAA AGCGCGACAG CACCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

side-VII ( NM_001275503.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262432.1 Drosophila melanogaster sidestep VII (side-VII), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04379
Clone ID Related Accession (Same CDS sequence)	NM_141676.3 , NM_001275503.1
Accession Version	NM_001275503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sidestep VII, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTTAC TGCTGCTGTT TATGCTAGCA CTTCAACTTA TAGAATCCAG CATCGGAAGT 
AAAGATGTAC CAATTCACCA AATTGAATCT ATAGTTGGTG AAAATATTTA TCTTCCTTGC 
AATGTAACAA CTTATGATGG TGATGAACCT GTGCTGGTTC TGTGGTACCG AGATGATAAG 
GGTACTCCAA TTTACAGCAT TGACATTCGA GCTGGAGTAT CCAAAGCACC GAAAAGATGG 
TCGGACGACT CCGTATTTGG GGATAGAGCA TATTTTATCT TTGACAAGGA ACCGGGAAAA 
CTGTCAATTC AGAATACGCA AGCATCCGAC TCCGGAACTT ATAGATGTCG CGTGGACTTC 
CTGAAAGCGC AGACCATAAA CAGCCGCATT AGGCTCAATG TCATATCTCC ACCCAAACAA 
GTTATTATTC GAGACAGCTC CAATGTTGAG CGTTCTACAG TTGTGGGACC ATATAGCGAA 
GGCGACATCG TATCCCTGAA ATGCCAAGTA ATTGGAGGCT ATCCCACGCC CACAATCAGT 
TGGTATCGCG ATGGCATAGA AATTCCATGC GAATTGTCTC ATTTGGCAGG CGGCAAAATT 
ATAGAATGCG AAATCACGCT ACCGTCTCTG GGTCGGGAGG ATCTTAACTC CCGGCTGACC 
TGTCGTGCAC TGAGTCACCC CCGGGCACCC ATCGTCGAAG CGGTGGTTCA AATCGATATG 
AATTTTGCCC CTTTAAACAT CCGACTGTTG GGGGCACATC AACCATTGTC TGCTGGACGC 
AGATACGATC TATTGTGCCA AAGTGCTGGC TCCAGGCCGC CGGCCGTAAT TACTTGGTGG 
CAAAATGGCA TACGGCTGGA GAAGACAACC GAAACGACTT CAAGCGATGG CAACCAGACC 
ACAAGCACTC TCTCAATCAG CCTAAGCAAA TCAGATGCGG GAAAGTATTT ATCATGCAAA 
GCCTACAACC ATGCCGTTCC CTCCGAGCCC CTGGAAGATG GCTGGAAATT AGATATACAA 
TATGTCCCGG AAGCGTACGT TCGTTTGGGA ACATCTCTGG ATCCGAGCAC TTTACGAGAA 
GGAACAGATG TCTACTTTGA TTGCCTGGTG ATGGCCCATC CAAATGTCTT TCGCATCGAA 
TGGCGTCACA ACGAACAACC TTTATCACAC AATATATCAC TGGGCGTCAT AATTAGTAAT 
CACTCGTTGG TACTGCAAGG TGTAACTAGA GCCACTGCGG GAAACTATTC CTGTGTGGGT 
TTCAATGCCG AAGGAGAAGG AATCAGTGCT CCCTTTGCAC TAAACATACT CTACGCCCCC 
ACGTGTGCCC AGAATCAGAA GAAGGTGTAC GGCATAGCCA AGCAGGAGGA CGCGAAGGTC 
ATGTGCACCG TGGACGCTAA TCCGCGCGAG GTCGAGTTTA GCTGGACATT TAATAATTCT 
GCCGAGAGTA TTGATGTCGC CACAAATCAT ATTATACGCT CGGGTACCAC CTCCATTGTA 
ACATACACAC CCATTACGGA ACTGGACTAC GGAACCCTTT TGTGTTTGGC GTCGAACAAA 
ATTGGCAAAC AGCGAGTGCC ATGTGTCTTC CATATAATCG CCGCAGGACG ACCGGAAAAA 
GTTCATAATT GCACGGTGAA CAATATATCT ATGACATCCT TGACGGTCAC TTGTAGTGAT 
GGTTTCAACG GCGGCTTGCC ACAGAGTTTT AATTTAGAAC TGCTGGACTC TTACACACAA 
GAAATTAAAG CTAATATAAC ATCCACTATT CCCAAGTTTA CGGTGCCAGG TTTGAGTCCG 
GGAAGTATAT ATAGGCTGAA TATTTACGCG TTCAATACGA AGGGTCGATC CGATCCAGCT 
ATAGTCAATG CGGCCATGCT GCGAATGCCC GAGAAGCAAC TGACCTCGGA ACAGACCCAC 
AATCTTCGCG CTGAGCTGCT ATTTTCACCA GTGATGTCCT TAACAATTGG CCTAACCCTC 
GCTGTGCTGG TCGCAGTTTT GGCCATCATT CTGGCACTTC GAATACCCTG TACCGCTTCA 
TCGAGACGGC GTCAAAAGGA ACTATCGAAT GAAAATATAT CCAGAGATGA GTCGCCTGGA 
CCAAGCGACA AGAGTTCCGG CAGCAAGGAA GTGGACGATT GCGATGAAAA GAATCCGGAT 
GTGGTACCCG AATCCATAGA ACCGGATGAG CAGGCCGAGA GCATCAGGAA AAGGCAGCAG 
ATATCGACAA TTGATACCAA TCGCAGTCCC AATCGAGGAA TTTTCGTGAA TGAGCAGCAG 
CATCTGACAG CAGCCGAGAT TTCCCTGCGT GCCTCGCATG GAATTGGATA CTGCACCCTG 
AGGAGTGGAA TGCATGCCCA GGCCCAGAGT TCGGTGGGCG AAATCTCAAT AAACGTAACG 
CCACACGTGT ACTCCAATTC GATCTCGCAA TGCACTCTGC CACGTCAGTC GTCGCAGAAC 
GTGTGGAACA ACTACAACTG CAACATCACC GGTGCGAGGC CGACCTCCAC GTTCCACCAG 
CTGACCTTTC CCCAGGGCAG GGTCAATGTC GGTGGTCACA ATCCGGCGCA GCCGCTCCAT 
GGACACGCAC CATCGCCGTC GCAGGCATCC AATAGCTCTA TGGCCTCGTC GACGAACACA 
TTGCCACAGC CACATCCACA TGGACGAACC ATCGCCATGC ACCACATGAA CCAGGCCCAG 
TGTGGCAGAG TCTGCATCGG AATCGCCAGC GATGGCATAC ACTCCGCCGA GCAGAATGTA 
TCCGACGACG AGGTCACCGT CCAAACTCCA TTAATGATAA AGCGCGACAG CACCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262432.1
CDS	660..3479
Translation	MELLLLFMLALQLIESSIGSKDVPIHQIESIVGENIYLPCNVTTYDGDEPVLVLWYRDDKGTPIYSIDIRAGVSKAPKRWSDDSVFGDRAYFIFDKEPGKLSIQNTQASDSGTYRCRVDFLKAQTINSRIRLNVISPPKQVIIRDSSNVERSTVVGPYSEGDIVSLKCQVIGGYPTPTISWYRDGIEIPCELSHLAGGKIIECEITLPSLGREDLNSRLTCRALSHPRAPIVEAVVQIDMNFAPLNIRLLGAHQPLSAGRRYDLLCQSAGSRPPAVITWWQNGIRLEKTTETTSSDGNQTTSTLSISLSKSDAGKYLSCKAYNHAVPSEPLEDGWKLDIQYVPEAYVRLGTSLDPSTLREGTDVYFDCLVMAHPNVFRIEWRHNEQPLSHNISLGVIISNHSLVLQGVTRATAGNYSCVGFNAEGEGISAPFALNILYAPTCAQNQKKVYGIAKQEDAKVMCTVDANPREVEFSWTFNNSAESIDVATNHIIRSGTTSIVTYTPITELDYGTLLCLASNKIGKQRVPCVFHIIAAGRPEKVHNCTVNNISMTSLTVTCSDGFNGGLPQSFNLELLDSYTQEIKANITSTIPKFTVPGLSPGSIYRLNIYAFNTKGRSDPAIVNAAMLRMPEKQLTSEQTHNLRAELLFSPVMSLTIGLTLAVLVAVLAIILALRIPCTASSRRRQKELSNENISRDESPGPSDKSSGSKEVDDCDEKNPDVVPESIEPDEQAESIRKRQQISTIDTNRSPNRGIFVNEQQHLTAAEISLRASHGIGYCTLRSGMHAQAQSSVGEISINVTPHVYSNSISQCTLPRQSSQNVWNNYNCNITGARPTSTFHQLTFPQGRVNVGGHNPAQPLHGHAPSPSQASNSSMASSTNTLPQPHPHGRTIAMHHMNQAQCGRVCIGIASDGIHSAEQNVSDDEVTVQTPLMIKRDSTV

Target ORF information:

RefSeq Version	NM_001275503.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sidestep VII (side-VII), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275503.1

ORF Insert Sequence:

ATGGAGTTAC TGCTGCTGTT TATGCTAGCA CTTCAACTTA TAGAATCCAG CATCGGAAGT 
AAAGATGTAC CAATTCACCA AATTGAATCT ATAGTTGGTG AAAATATTTA TCTTCCTTGC 
AATGTAACAA CTTATGATGG TGATGAACCT GTGCTGGTTC TGTGGTACCG AGATGATAAG 
GGTACTCCAA TTTACAGCAT TGACATTCGA GCTGGAGTAT CCAAAGCACC GAAAAGATGG 
TCGGACGACT CCGTATTTGG GGATAGAGCA TATTTTATCT TTGACAAGGA ACCGGGAAAA 
CTGTCAATTC AGAATACGCA AGCATCCGAC TCCGGAACTT ATAGATGTCG CGTGGACTTC 
CTGAAAGCGC AGACCATAAA CAGCCGCATT AGGCTCAATG TCATATCTCC ACCCAAACAA 
GTTATTATTC GAGACAGCTC CAATGTTGAG CGTTCTACAG TTGTGGGACC ATATAGCGAA 
GGCGACATCG TATCCCTGAA ATGCCAAGTA ATTGGAGGCT ATCCCACGCC CACAATCAGT 
TGGTATCGCG ATGGCATAGA AATTCCATGC GAATTGTCTC ATTTGGCAGG CGGCAAAATT 
ATAGAATGCG AAATCACGCT ACCGTCTCTG GGTCGGGAGG ATCTTAACTC CCGGCTGACC 
TGTCGTGCAC TGAGTCACCC CCGGGCACCC ATCGTCGAAG CGGTGGTTCA AATCGATATG 
AATTTTGCCC CTTTAAACAT CCGACTGTTG GGGGCACATC AACCATTGTC TGCTGGACGC 
AGATACGATC TATTGTGCCA AAGTGCTGGC TCCAGGCCGC CGGCCGTAAT TACTTGGTGG 
CAAAATGGCA TACGGCTGGA GAAGACAACC GAAACGACTT CAAGCGATGG CAACCAGACC 
ACAAGCACTC TCTCAATCAG CCTAAGCAAA TCAGATGCGG GAAAGTATTT ATCATGCAAA 
GCCTACAACC ATGCCGTTCC CTCCGAGCCC CTGGAAGATG GCTGGAAATT AGATATACAA 
TATGTCCCGG AAGCGTACGT TCGTTTGGGA ACATCTCTGG ATCCGAGCAC TTTACGAGAA 
GGAACAGATG TCTACTTTGA TTGCCTGGTG ATGGCCCATC CAAATGTCTT TCGCATCGAA 
TGGCGTCACA ACGAACAACC TTTATCACAC AATATATCAC TGGGCGTCAT AATTAGTAAT 
CACTCGTTGG TACTGCAAGG TGTAACTAGA GCCACTGCGG GAAACTATTC CTGTGTGGGT 
TTCAATGCCG AAGGAGAAGG AATCAGTGCT CCCTTTGCAC TAAACATACT CTACGCCCCC 
ACGTGTGCCC AGAATCAGAA GAAGGTGTAC GGCATAGCCA AGCAGGAGGA CGCGAAGGTC 
ATGTGCACCG TGGACGCTAA TCCGCGCGAG GTCGAGTTTA GCTGGACATT TAATAATTCT 
GCCGAGAGTA TTGATGTCGC CACAAATCAT ATTATACGCT CGGGTACCAC CTCCATTGTA 
ACATACACAC CCATTACGGA ACTGGACTAC GGAACCCTTT TGTGTTTGGC GTCGAACAAA 
ATTGGCAAAC AGCGAGTGCC ATGTGTCTTC CATATAATCG CCGCAGGACG ACCGGAAAAA 
GTTCATAATT GCACGGTGAA CAATATATCT ATGACATCCT TGACGGTCAC TTGTAGTGAT 
GGTTTCAACG GCGGCTTGCC ACAGAGTTTT AATTTAGAAC TGCTGGACTC TTACACACAA 
GAAATTAAAG CTAATATAAC ATCCACTATT CCCAAGTTTA CGGTGCCAGG TTTGAGTCCG 
GGAAGTATAT ATAGGCTGAA TATTTACGCG TTCAATACGA AGGGTCGATC CGATCCAGCT 
ATAGTCAATG CGGCCATGCT GCGAATGCCC GAGAAGCAAC TGACCTCGGA ACAGACCCAC 
AATCTTCGCG CTGAGCTGCT ATTTTCACCA GTGATGTCCT TAACAATTGG CCTAACCCTC 
GCTGTGCTGG TCGCAGTTTT GGCCATCATT CTGGCACTTC GAATACCCTG TACCGCTTCA 
TCGAGACGGC GTCAAAAGGA ACTATCGAAT GAAAATATAT CCAGAGATGA GTCGCCTGGA 
CCAAGCGACA AGAGTTCCGG CAGCAAGGAA GTGGACGATT GCGATGAAAA GAATCCGGAT 
GTGGTACCCG AATCCATAGA ACCGGATGAG CAGGCCGAGA GCATCAGGAA AAGGCAGCAG 
ATATCGACAA TTGATACCAA TCGCAGTCCC AATCGAGGAA TTTTCGTGAA TGAGCAGCAG 
CATCTGACAG CAGCCGAGAT TTCCCTGCGT GCCTCGCATG GAATTGGATA CTGCACCCTG 
AGGAGTGGAA TGCATGCCCA GGCCCAGAGT TCGGTGGGCG AAATCTCAAT AAACGTAACG 
CCACACGTGT ACTCCAATTC GATCTCGCAA TGCACTCTGC CACGTCAGTC GTCGCAGAAC 
GTGTGGAACA ACTACAACTG CAACATCACC GGTGCGAGGC CGACCTCCAC GTTCCACCAG 
CTGACCTTTC CCCAGGGCAG GGTCAATGTC GGTGGTCACA ATCCGGCGCA GCCGCTCCAT 
GGACACGCAC CATCGCCGTC GCAGGCATCC AATAGCTCTA TGGCCTCGTC GACGAACACA 
TTGCCACAGC CACATCCACA TGGACGAACC ATCGCCATGC ACCACATGAA CCAGGCCCAG 
TGTGGCAGAG TCTGCATCGG AATCGCCAGC GATGGCATAC ACTCCGCCGA GCAGAATGTA 
TCCGACGACG AGGTCACCGT CCAAACTCCA TTAATGATAA AGCGCGACAG CACCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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side-VII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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