betaTub85D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	betaTub85D
Entrez Gene ID	41124
Full Name	beta-Tubulin at 85D
Synonyms	2t,B2t,BETA 85D,BETA2,BetaTub85D,CG9359,D.m.BETA-85D,DTB4,Dmbeta2,Dmel\CG9359,Tub,Tub2A,beta(2)Tu,beta(2)Tub,beta-Tub85D,beta-tub,beta-tub85D,beta2,beta2-tub,beta2t,beta2tub,beta85D,beta;Tub85D,betaTub2,beta[[2]]-tubulin,betatub85D,ms(3)KK[D]
General protein information	Preferred Names beta-Tubulin at 85D Names CG9359 gene product from transcript CG9359-RA CG9359-PA b2 tubulin beta 2 tubulin beta tubulin beta(2)Tubulin beta-Tubulin beta-Tubulin 85D beta-tubulin85D beta2 tubulin beta2-tubulin beta2tubulin betaTub85D-PA betaTubulin85D testis-specific beta-tubulin tubulin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85D15-85D15\|3-48.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079566.4	NP_524290.2	beta-Tubulin at 85D

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TUBB2B	NP_001003900.1
Oryza sativa (rice)	Os03g0661300	NP_001050825.1
Homo sapiens (human)	TUBB2B	NP_821080.1
Pan troglodytes (chimpanzee)	TUBB2B	XP_001162039.1
	TUBB2B	XP_001092923.1
Canis lupus familiaris (dog)	TUBB2B	XP_856151.2
Mus musculus (house mouse)	Tubb2b	NP_076205.1
Gallus gallus (chicken)	LOC768337	XP_001231210.2
Caenorhabditis elegans (roundworm)	mec-7	NP_509313.1
Rattus norvegicus (Norway rat)	Tubb2b	NP_001013908.2
Drosophila melanogaster (fruit fly)	betaTub85D	NP_524290.2
Xenopus tropicalis (tropical clawed frog)	tubb2b	NP_989275.1
Gallus gallus (chicken)	TUBB2B	NP_001004400.1
Arabidopsis thaliana (thale cress)	TUB2	NP_568959.1
Danio rerio (zebrafish)	zgc:123194	NP_001032487.1
Arabidopsis thaliana (thale cress)	TUB3	NP_568960.1
Arabidopsis thaliana (thale cress)	TUB8	NP_001190373.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

beta2t plays an important role in salivary gland and myoblast migration
Title: Deficiency screen identifies a novel role for beta 2 tubulin in salivary gland and myoblast migration in the Drosophila embryo.

Partially truncated beta2-tubulin (beta2Delta7) containing only the proximal portion of the C-terminal tail, including the axoneme motif, can support production of functional motile sperm.
Title: The proximal region of the beta-tubulin C-terminal tail is sufficient for axoneme assembly.

The following betaTub85D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the betaTub85D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08205	NM_079566.4 Latest version!	Drosophila melanogaster beta-Tubulin at 85D (betaTub85D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

betaTub85D ( NM_079566.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524290.2 Drosophila melanogaster beta-Tubulin at 85D (betaTub85D), mRNA.

Price & Availability↑

CloneID	OFa08205
Clone ID Related Accession (Same CDS sequence)	NM_079566.4
Accession Version	NM_079566.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta-Tubulin at 85D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_079566.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAAA TTGTACACAT TCAGGCCGGT CAATGCGGTA ACCAGATCGG TGGTAAATTC 
TGGGAGGTAA TCTCGGATGA GCACTGTATA GATGCGACCG GAACGTACTA CGGCGATAGT 
GATCTCCAGC TGGAGCGCAT CAATGTATAC TACAATGAAG CCACCGGTGC CAAGTATGTG 
CCACGCGCAA TTCTCGTGGA CCTGGAGCCC GGCACCATGG ATTCGGTTCG TTCTGGCGCC 
TTTGGCCAGA TCTTCCGGCC GGACAATTTT GTGTTTGGCC AATCGGGAGC AGGCAACAAC 
TGGGCCAAGG GTCATTACAC CGAGGGTGCT GAACTGGTGG ATTCCGTCTT GGATGTGGTG 
CGAAAGGAGT CCGAGGGATG CGATTGCCTT CAGGGCTTCC AGCTGACCCA CTCGCTGGGT 
GGCGGCACTG GCTCCGGCAT GGGAACCCTG CTGATCTCGA AGATCCGCGA GGAGTACCCG 
GACCGCATCA TGAACACCTT CTCGGTGGTG CCCTCGCCCA AGGTGTCCGA TACGGTGGTG 
GAGCCCTACA ATGCCACCCT GAGTGTGCAT CAGCTGGTGG AGAACACCGA TGAGACGTAC 
TGCATCGACA ACGAGGCGTT GTATGACATC TGCTTCCGCA CACTGAAGCT GACCACGCCC 
ACCTACGGTG ACCTGAACCA TCTGGTTTCG GCCACCATGT CTGGTGTGAC GACCTGCCTG 
CGCTTCCCTG GCCAGCTGAA CGCTGATCTT CGCAAGCTGG CCGTGAACAT GGTACCCTTC 
CCCCGGCTGC ACTTCTTCAT GCCCGGATTC GCACCGCTCA CCTCGCGAGG ATCGCAACAA 
TACCGGGCCC TTACCGTTCC GGAGCTGACC CAGCAGATGT TCGATGCCAA GAACATGATG 
GCTGCGTGCG ATCCGCGACA TGGTCGCTAT CTGACCGTCG CCGCCATCTT CCGTGGCCGC 
ATGTCCATGA AGGAGGTGGA CGAGCAGATG CTCAACATTC AGAACAAGAA CAGCAGCTTC 
TTCGTGGAAT GGATCCCGAA TAACTGCAAG ACAGCGGTGT GCGATATTCC GCCCAGAGGT 
CTCAAGATGT CGGCCACCTT CATTGGCAAC TCCACCGCCA TTCAGGAGCT ATTCAAACGG 
GTTTCGGAGC AGTTCACCGC CATGTTCCGA AGGAAGGCCT TCTTGCATTG GTACACCGGC 
GAGGGAATGG ACGAAATGGA ATTCACAGAG GCCGAGAGCA ACATGAACGA CTTGGTTTCT 
GAATATCAGC AGTACCAGGA GGCGACTGCC GATGAGGAGG GCGAATTCGA TGAAGACGAA 
GAGGGTGGCG GCGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524290.2
CDS	231..1571
Translation	MREIVHIQAGQCGNQIGGKFWEVISDEHCIDATGTYYGDSDLQLERINVYYNEATGAKYVPRAILVDLEPGTMDSVRSGAFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSFFVEWIPNNCKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATADEEGEFDEDEEGGGDE

Target ORF information:

RefSeq Version	NM_079566.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta-Tubulin at 85D (betaTub85D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079566.4

ORF Insert Sequence:

ATGCGTGAAA TTGTACACAT TCAGGCCGGT CAATGCGGTA ACCAGATCGG TGGTAAATTC 
TGGGAGGTAA TCTCGGATGA GCACTGTATA GATGCGACCG GAACGTACTA CGGCGATAGT 
GATCTCCAGC TGGAGCGCAT CAATGTATAC TACAATGAAG CCACCGGTGC CAAGTATGTG 
CCACGCGCAA TTCTCGTGGA CCTGGAGCCC GGCACCATGG ATTCGGTTCG TTCTGGCGCC 
TTTGGCCAGA TCTTCCGGCC GGACAATTTT GTGTTTGGCC AATCGGGAGC AGGCAACAAC 
TGGGCCAAGG GTCATTACAC CGAGGGTGCT GAACTGGTGG ATTCCGTCTT GGATGTGGTG 
CGAAAGGAGT CCGAGGGATG CGATTGCCTT CAGGGCTTCC AGCTGACCCA CTCGCTGGGT 
GGCGGCACTG GCTCCGGCAT GGGAACCCTG CTGATCTCGA AGATCCGCGA GGAGTACCCG 
GACCGCATCA TGAACACCTT CTCGGTGGTG CCCTCGCCCA AGGTGTCCGA TACGGTGGTG 
GAGCCCTACA ATGCCACCCT GAGTGTGCAT CAGCTGGTGG AGAACACCGA TGAGACGTAC 
TGCATCGACA ACGAGGCGTT GTATGACATC TGCTTCCGCA CACTGAAGCT GACCACGCCC 
ACCTACGGTG ACCTGAACCA TCTGGTTTCG GCCACCATGT CTGGTGTGAC GACCTGCCTG 
CGCTTCCCTG GCCAGCTGAA CGCTGATCTT CGCAAGCTGG CCGTGAACAT GGTACCCTTC 
CCCCGGCTGC ACTTCTTCAT GCCCGGATTC GCACCGCTCA CCTCGCGAGG ATCGCAACAA 
TACCGGGCCC TTACCGTTCC GGAGCTGACC CAGCAGATGT TCGATGCCAA GAACATGATG 
GCTGCGTGCG ATCCGCGACA TGGTCGCTAT CTGACCGTCG CCGCCATCTT CCGTGGCCGC 
ATGTCCATGA AGGAGGTGGA CGAGCAGATG CTCAACATTC AGAACAAGAA CAGCAGCTTC 
TTCGTGGAAT GGATCCCGAA TAACTGCAAG ACAGCGGTGT GCGATATTCC GCCCAGAGGT 
CTCAAGATGT CGGCCACCTT CATTGGCAAC TCCACCGCCA TTCAGGAGCT ATTCAAACGG 
GTTTCGGAGC AGTTCACCGC CATGTTCCGA AGGAAGGCCT TCTTGCATTG GTACACCGGC 
GAGGGAATGG ACGAAATGGA ATTCACAGAG GCCGAGAGCA ACATGAACGA CTTGGTTTCT 
GAATATCAGC AGTACCAGGA GGCGACTGCC GATGAGGAGG GCGAATTCGA TGAAGACGAA 
GAGGGTGGCG GCGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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betaTub85D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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