pum cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pum
Entrez Gene ID	41094
Full Name	pumilio
Synonyms	CG9755,CG9763,Dmel\CG9755,EP(3)0883,PKL,PUM,Pum,anon-WO0172774.19,bem,fs(3)02003,l(3)01688,ova,ovt,pkl
General protein information	Preferred Names pumilio Names CG9755 gene product from transcript CG9755-RG CG9755-PA CG9755-PB CG9755-PC CG9755-PD CG9755-PE CG9755-PF CG9755-PG CG9755-PH bemused milord ovarette pum-PA pum-PB pum-PC pum-PD pum-PE pum-PF pum-PG pum-PH pumillio pumuckel
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85C4-85D1\|3-48.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_169260.4	NP_731316.2	pumilio, isoform B
NM_001260073.2	NP_001247002.1	pumilio, isoform F
NM_001275474.1	NP_001262403.1	pumilio, isoform G
NM_169258.3	NP_731314.1	pumilio, isoform A
NM_001275475.1	NP_001262404.1	pumilio, isoform H
NM_169259.3	NP_731315.1	pumilio, isoform D
NM_079561.4	NP_524285.2	pumilio, isoform C
NM_176427.3	NP_788604.1	pumilio, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Crystal structures of Nanos-Pumilio-RNA complexes reveal that Nanos embraces Pumilio and RNA, contributes sequence-specific contacts, and increases Pumilio RNA-binding affinity.
Title: Drosophila Nanos acts as a molecular clamp that modulates the RNA-binding and repression activities of Pumilio.

reductions in the expression of the Pumilio translational repressor complex enhanced phenotypes due to dLsd1 depletion in Drosophila. the PUM complex is a target of LSD1 regulation.
Title: The LSD1 Family of Histone Demethylases and the Pumilio Posttranscriptional Repressor Function in a Complex Regulatory Feedback Loop.

BRAT is a prominent post-transcriptional regulator in the early embryo through mechanisms that are largely independent of PUM.
Title: Brain tumor is a sequence-specific RNA-binding protein that directs maternal mRNA clearance during the Drosophila maternal-to-zygotic transition.

Pumilio has a novel mRNA decay function during neural development. Decreased expression stabilized predicted neural mRNA targets. A Pumilio recognition element is needed to trigger reporter-transcript decay in the nervous system.
Title: Dynamic regulation of mRNA decay during neural development.

Evidence suggests that Pum functions as a negative regulator of EGFR signaling by directly targeting components of the pathway in Drosophila.
Title: Negative regulation of EGFR/MAPK pathway by Pumilio in Drosophila melanogaster.

The following pum gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pum cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04317	NM_169258.3 Latest version!	Drosophila melanogaster pumilio (pum), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa04317	NM_079561.4 Latest version!	Drosophila melanogaster pumilio (pum), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa04317	NM_001275474.1 Latest version!	Drosophila melanogaster pumilio (pum), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa04317	NM_169259.3 Latest version!	Drosophila melanogaster pumilio (pum), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa04318	NM_001275475.1 Latest version!	Drosophila melanogaster pumilio (pum), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa04319	NM_001260073.2 Latest version!	Drosophila melanogaster pumilio (pum), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa08171	NM_169260.4 Latest version!	Drosophila melanogaster pumilio (pum), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa08172	NM_176427.3 Latest version!	Drosophila melanogaster pumilio (pum), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pum ( NM_001275474.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262403.1 Drosophila melanogaster pumilio (pum), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa04317
Clone ID Related Accession (Same CDS sequence)	NM_001275474.1 , NM_169258.3 , NM_169259.3 , NM_079561.4
Accession Version	NM_001275474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pumilio, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTTT TGGGTGGTAA CGATGATCGC AATGGCCGCG GAGGCGTCGG CGTTGGCACG 
GATGCCATTG TAGGATCTCG AGGTGGCGTC TCTCAGGATG CCGCCGATGC AGCTGGTGCC 
GCCGCAGCCG CCGCCGTCGG CTATGTCTTC CAGCAGCGTC CATCGCCTGG TGGGGTTGGC 
GTCGGCGTGG GCGGAGTGGG TGGCGGTGTG CCAGGGGTCG GAGCCGTAGG CTCGACCTTG 
CACGAGGCCG CCGCCGCCGA GTACGCCGCC CACTTTGCCC AGAAGCAACA GCAGACCCGA 
TGGGCGTGCG GCGACGACGG CCATGGGATC GATAACCCGG ACAAATGGAA GTACAATCCG 
CCGATGAATC CGGCCAATGC CGCTCCTGGC GGTCCACCGG GAAATGGCAG TAATGGTGGG 
CCCGGCGCCA TTGGAACCAT TGGCATGGGC AGCGGATTGG GTGGTGGTGG CGGCGGCGGA 
GCTGGCGGCG GAAATAATGG CGGCTCTGGT ACGAATGGCG GTCTGCATCA TCAATCGATG 
GCCGCTGCAG CTGCGAATAT GGCAGCCATG CAACAGGCGG CGGCGTTGGC CAAGCACAAT 
CACATGATAT CACAGGCAGC AGCCGCAGTT GCAGCCCAGC AACAACATCA GCATCCACAC 
CAGCAGCATC CCCAGCAGCA GCAGCAACAG CAGCAGGCGC AGAACCAGGG GCATCCACAT 
CACCTTATGG GCGGTGGCAA TGGACTGGGC AACGGCAATG GATTGGGCAT ACAACATCCC 
GGCCAGCAAC AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACATCCCGG CCAGTACAAC 
GCGAATCTGC TTAACCATGC GGCTGCCTTG GGTCACATGT CATCTTATGC CCAATCGGGT 
GGCAGCATGT ACGACCATCA TGGTGGAGCC ATGCACCCGG GAATGAACGG CGGCATGCCC 
AAGCAACAGC CATTGGGTCC ACCCGGAGCC GGAGGACCCC AGGACTATGT CTACATGGGT 
GGCCAGACCA CTGTGCCCAT GGGAGCCGCA ATGATGCCGC CACAGAATCA ATATATGAAC 
AGCTCTGCTG TTGCAGCTGC CAATCGGAAT GCAGCGATTA CCACATCCAC TGCCAAGAAA 
TTGTGGGAGA AATCCGATGG CAAGGGCGTA TCCTCGAGCA CTCCCGGTGG ACCGTTGCAT 
CCCCTGCAGA TCCCCGGCAT CGGGGATCCC TCCTCCGTGT GGAAGGATCA CACCTGGTCC 
ACACAGGGCG AGAATATATT GGTGCCGCCC CCCTCGCGAG CCTACGCCCA TGGAGGCGCC 
TCCGATACTT CAAACAGCGG CAATGCGGGC ATACTGAGTC CCCGCGATTC GACTTGCGCC 
AAAGTGGTTG AATATGTTTT CAGTGGCTCG CCCACCAACA AAGATAGCTC GCTTTCCGGA 
TTGGAACCGC ATTTGCGGAA TCTAAAGTTT GACGACAACG ATAAGTCACG CGACGATAAG 
GAGAAAGCAA ACTCTCCGTT TGACACAAAC GGTTTGAAGA AAGACGATCA GGTCACAAAC 
TCAAATGGTG TTGTCAACGG CATTGACGAT GACAAGGGCT TCAATCGCAC TCCTGGTTCA 
CGTCAACCAT CACCTGCAGA GGAGTCCCAG CCACGTCCCC CCAATCTACT CTTTCCTCCA 
CTGCCCTTCA ATCACATGCT CATGGATCAT GGCCAAGGCA TGGGAGGCGG CTTGGGCGGA 
GTTGTTGGAT CTGGCAACGG AGTCGGCGGT GGCAGCGGCG GAGGCGGGGC AGGCGGCGCT 
TATGCGGCCC ACCAGCAGAT GGCCGCCCAG ATGAGTCAAT TGCAACCGCC GATGATGAAC 
GGCGTTGGCG GCGGAATGCC AATGGCAGCA CAGTCACCAA TGTTGAATCA CCAGGCAGCT 
GGACCCAATC ACATGGAATC TCCCGGAAAT CTCTTGCAGC AGCAAAATTT TGATGTTCAG 
CAACTGTTTC GCTCGCAGAA TCCGGGCCTA GCAGCAGTTG CCACAAATGC AGCGGCCGCA 
GCAGCAGCCG CAGCAGCTGC CACATCGGCA GCGAGTGCTG CGGCAGCGGT GGGCGCACCA 
CCCGTTCCCA ACGGATCGCT GCAGCAGTCG CAGCAGCAAC AGCAGCAGCA GCAACAACAG 
CAGCAGCAAC AACAGATGCA CATGGCGGCC GCGTCGCAAC AATTTTTGGC CGCCCAGCAG 
CAGGCGCAAA ATGCGGCCTA TGCCGCCCAA CAGGCCACGT CCTACGTCAT CAATCCGGGC 
CAGGAGGCTG CCCCGTATAT GGGCATGATT GCCGCCGCCC AGATGCCGTA CTATGGCGTA 
GCACCATGGG GCATGTATCC GGGCAATCTG ATTCCGCAAC AGGGAACGCA GCCGCGCCGC 
CCCCTCACCC CCTCGCAGCA GGGTGCCGAG AATCAGCCGT ATCAGGTCAT CCCGGCATTC 
CTCGATCACA CGGGCTCCTT GCTGATGGGA GGACCTCGCA CCGGGACGCC GATGCGTCTG 
GTTAGCCCCG CCCCCGTTCT GGTGCCCCCG GGCGCTACCC GTGCCGGCCC CCCGCCCCCG 
CAGGGGCCAC AGCTGTATCA GCCGCAGCCG CAGACGGCCC AACAGAATCT CTACTCGCAG 
CAGAATGGAT CCAGTGTCGG AGGCCTCGCC TTGAACACGA GCTCGTTGAC GGGTCGCCGC 
GACTCCTTCG ACCGCAGCAC CTCCGCCTTC AGTCCCTCGA CCATGGACTA CACCAGCAGC 
GGTGTGGCAG CGGCCGCCAA TGCGGTGAAC AGCACAGTGG CCCAGGCAGC AGCAGCTGCC 
GCAGCAGCCG CCGCAGCGCG TGGCAAGTGG CCGGGAGCGA TGTCGGGAGC GGCCAGTGGA 
GCCTACGGAG CCCTGGGAGC GGGCAATGCT TCGGCCAGTC CCCTGGGCGC ACCAATCACG 
CCGCCGCCAT CGGCGCAATC CTGTCTCCTG GGCAGTCGGG CACCTGGAGC CGAGTCCCGC 
CAGCGGCAGC AGCAACAACA GCAGCTGGCC GCCGTTGGTC TGCCGGCGAC TGCAGCAGCT 
GCTCAGGCAG CGGTGGCCGC GGCTGCCAAC AATATGTTCG GATCCAACAG CTCGATCTTC 
TCGAATCCCC TGGCCATTCC GGGTACCGCA GCTGTGGCAG CTGCAGCGGC AGCAGCAGCG 
GCCGCCAACT CGCGTCAGGT GGCTGCCACG GCAGCGGCAG CAGCGGCGGT GGCAGCAGCA 
GCCGGCGGAG TGGGAGGTGC CCCACAGCCA GGAAGATCTC GCCTTCTCGA AGATTTCCGC 
AACCAGCGGT ATCCAAATCT TCAGCTACGC GATCTCGCTA ACCACATTGT GGAGTTCTCA 
CAGGATCAGC ACGGCTCGCG GTTTATCCAA CAGAAGTTGG AGCGGGCCAC CGCCGCCGAG 
AAGCAAATGG TGTTCAGCGA GATCCTGGCG GCAGCCTATA GCCTGATGAC CGATGTCTTT 
GGCAACTATG TCATCCAGAA GTTCTTTGAG TTCGGCACTC CCGAGCAGAA GAACACGCTG 
GGCATGCAGG TCAAGGGTCA TGTGCTGCAG CTGGCGCTGC AAATGTATGG CTGCCGAGTG 
ATTCAGAAGG CTCTGGAGAG CATCTCGCCG GAGCAGCAGC AGGAAATCGT CCACGAACTG 
GACGGACATG TGCTGAAATG CGTCAAGGAT CAGAATGGCA ATCATGTGGT GCAAAAGTGC 
ATTGAGTGCG TGGACCCCGT GGCGCTGCAG TTCATCATCA ATGCTTTCAA GGGTCAGGTT 
TACTCGCTAA GCACCCATCC GTATGGATGC CGGGTGATCC AGAGAATCCT TGAGCATTGC 
ACTGCCGAAC AGACCACGCC CATTTTGGAC GAACTGCATG AGCACACCGA ACAGTTGATT 
CAGGACCAAT ATGGCAACTA TGTTATTCAG CATGTGCTTG AACACGGCAA GCAGGAGGAT 
AAGTCGATTC TTATCAACAG CGTGCGCGGC AAAGTTCTGG TGCTATCACA GCACAAGTTC 
GCCTCAAACG TTGTGGAGAA ATGTGTTACC CATGCCACTC GCGGAGAACG CACTGGTCTC 
ATAGACGAGG TCTGCACCTT CAACGACAAC GCGTTGCACG TGATGATGAA GGATCAGTAT 
GCCAACTATG TGGTCCAAAA AATGATCGAT GTATCGGAGC CGACGCAGCT CAAGAAGCTG 
ATGACCAAGA TCCGGCCCCA CATGGCCGCC TTGCGCAAGT ACACCTACGG CAAGCACATC 
AATGCCAAGT TGGAGAAGTA CTACATGAAG ATAACCAATC CCATTACGGT GGGCACAGGA 
GCTGGAGGAG TGCCGGCAGC CTCGTCGGCG GCGGCAGTCA GCAGTGGTGC CACCTCGGCA 
TCGGTAACCG CCTGCACCAG TGGCAGCAGC ACCACCACGA CCAGCACTAC CAACAGCCTG 
GCCTCACCCA CCATTTGTTC GGTGCAGGAG AACGGCAGCG CCATGGTTGT GGAGCCCTCC 
TCCCCGGACG CCTCCGAGTC CTCGTCCTCG GTGGTGTCAG GCGCTGTCAA CAGCAGCTTG 
GGTCCCATTG GACCCCCGAC CAACGGCAAC GTTGTGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262403.1
CDS	1020..5621
Translation	MKFLGGNDDRNGRGGVGVGTDAIVGSRGGVSQDAADAAGAAAAAAVGYVFQQRPSPGGVGVGVGGVGGGVPGVGAVGSTLHEAAAAEYAAHFAQKQQQTRWACGDDGHGIDNPDKWKYNPPMNPANAAPGGPPGNGSNGGPGAIGTIGMGSGLGGGGGGGAGGGNNGGSGTNGGLHHQSMAAAAANMAAMQQAAALAKHNHMISQAAAAVAAQQQHQHPHQQHPQQQQQQQQAQNQGHPHHLMGGGNGLGNGNGLGIQHPGQQQQQQQQQQQQQHPGQYNANLLNHAAALGHMSSYAQSGGSMYDHHGGAMHPGMNGGMPKQQPLGPPGAGGPQDYVYMGGQTTVPMGAAMMPPQNQYMNSSAVAAANRNAAITTSTAKKLWEKSDGKGVSSSTPGGPLHPLQIPGIGDPSSVWKDHTWSTQGENILVPPPSRAYAHGGASDTSNSGNAGILSPRDSTCAKVVEYVFSGSPTNKDSSLSGLEPHLRNLKFDDNDKSRDDKEKANSPFDTNGLKKDDQVTNSNGVVNGIDDDKGFNRTPGSRQPSPAEESQPRPPNLLFPPLPFNHMLMDHGQGMGGGLGGVVGSGNGVGGGSGGGGAGGAYAAHQQMAAQMSQLQPPMMNGVGGGMPMAAQSPMLNHQAAGPNHMESPGNLLQQQNFDVQQLFRSQNPGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQQQQQQMHMAAASQQFLAAQQQAQNAAYAAQQATSYVINPGQEAAPYMGMIAAAQMPYYGVAPWGMYPGNLIPQQGTQPRRPLTPSQQGAENQPYQVIPAFLDHTGSLLMGGPRTGTPMRLVSPAPVLVPPGATRAGPPPPQGPQLYQPQPQTAQQNLYSQQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPSTMDYTSSGVAAAANAVNSTVAQAAAAAAAAAAARGKWPGAMSGAASGAYGALGAGNASASPLGAPITPPPSAQSCLLGSRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCTFNDNALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLEKYYMKITNPITVGTGAGGVPAASSAAAVSSGATSASVTACTSGSSTTTTSTTNSLASPTICSVQENGSAMVVEPSSPDASESSSSVVSGAVNSSLGPIGPPTNGNVVL

Target ORF information:

RefSeq Version	NM_001275474.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pumilio (pum), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275474.1

ORF Insert Sequence:

ATGAAGTTTT TGGGTGGTAA CGATGATCGC AATGGCCGCG GAGGCGTCGG CGTTGGCACG 
GATGCCATTG TAGGATCTCG AGGTGGCGTC TCTCAGGATG CCGCCGATGC AGCTGGTGCC 
GCCGCAGCCG CCGCCGTCGG CTATGTCTTC CAGCAGCGTC CATCGCCTGG TGGGGTTGGC 
GTCGGCGTGG GCGGAGTGGG TGGCGGTGTG CCAGGGGTCG GAGCCGTAGG CTCGACCTTG 
CACGAGGCCG CCGCCGCCGA GTACGCCGCC CACTTTGCCC AGAAGCAACA GCAGACCCGA 
TGGGCGTGCG GCGACGACGG CCATGGGATC GATAACCCGG ACAAATGGAA GTACAATCCG 
CCGATGAATC CGGCCAATGC CGCTCCTGGC GGTCCACCGG GAAATGGCAG TAATGGTGGG 
CCCGGCGCCA TTGGAACCAT TGGCATGGGC AGCGGATTGG GTGGTGGTGG CGGCGGCGGA 
GCTGGCGGCG GAAATAATGG CGGCTCTGGT ACGAATGGCG GTCTGCATCA TCAATCGATG 
GCCGCTGCAG CTGCGAATAT GGCAGCCATG CAACAGGCGG CGGCGTTGGC CAAGCACAAT 
CACATGATAT CACAGGCAGC AGCCGCAGTT GCAGCCCAGC AACAACATCA GCATCCACAC 
CAGCAGCATC CCCAGCAGCA GCAGCAACAG CAGCAGGCGC AGAACCAGGG GCATCCACAT 
CACCTTATGG GCGGTGGCAA TGGACTGGGC AACGGCAATG GATTGGGCAT ACAACATCCC 
GGCCAGCAAC AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACATCCCGG CCAGTACAAC 
GCGAATCTGC TTAACCATGC GGCTGCCTTG GGTCACATGT CATCTTATGC CCAATCGGGT 
GGCAGCATGT ACGACCATCA TGGTGGAGCC ATGCACCCGG GAATGAACGG CGGCATGCCC 
AAGCAACAGC CATTGGGTCC ACCCGGAGCC GGAGGACCCC AGGACTATGT CTACATGGGT 
GGCCAGACCA CTGTGCCCAT GGGAGCCGCA ATGATGCCGC CACAGAATCA ATATATGAAC 
AGCTCTGCTG TTGCAGCTGC CAATCGGAAT GCAGCGATTA CCACATCCAC TGCCAAGAAA 
TTGTGGGAGA AATCCGATGG CAAGGGCGTA TCCTCGAGCA CTCCCGGTGG ACCGTTGCAT 
CCCCTGCAGA TCCCCGGCAT CGGGGATCCC TCCTCCGTGT GGAAGGATCA CACCTGGTCC 
ACACAGGGCG AGAATATATT GGTGCCGCCC CCCTCGCGAG CCTACGCCCA TGGAGGCGCC 
TCCGATACTT CAAACAGCGG CAATGCGGGC ATACTGAGTC CCCGCGATTC GACTTGCGCC 
AAAGTGGTTG AATATGTTTT CAGTGGCTCG CCCACCAACA AAGATAGCTC GCTTTCCGGA 
TTGGAACCGC ATTTGCGGAA TCTAAAGTTT GACGACAACG ATAAGTCACG CGACGATAAG 
GAGAAAGCAA ACTCTCCGTT TGACACAAAC GGTTTGAAGA AAGACGATCA GGTCACAAAC 
TCAAATGGTG TTGTCAACGG CATTGACGAT GACAAGGGCT TCAATCGCAC TCCTGGTTCA 
CGTCAACCAT CACCTGCAGA GGAGTCCCAG CCACGTCCCC CCAATCTACT CTTTCCTCCA 
CTGCCCTTCA ATCACATGCT CATGGATCAT GGCCAAGGCA TGGGAGGCGG CTTGGGCGGA 
GTTGTTGGAT CTGGCAACGG AGTCGGCGGT GGCAGCGGCG GAGGCGGGGC AGGCGGCGCT 
TATGCGGCCC ACCAGCAGAT GGCCGCCCAG ATGAGTCAAT TGCAACCGCC GATGATGAAC 
GGCGTTGGCG GCGGAATGCC AATGGCAGCA CAGTCACCAA TGTTGAATCA CCAGGCAGCT 
GGACCCAATC ACATGGAATC TCCCGGAAAT CTCTTGCAGC AGCAAAATTT TGATGTTCAG 
CAACTGTTTC GCTCGCAGAA TCCGGGCCTA GCAGCAGTTG CCACAAATGC AGCGGCCGCA 
GCAGCAGCCG CAGCAGCTGC CACATCGGCA GCGAGTGCTG CGGCAGCGGT GGGCGCACCA 
CCCGTTCCCA ACGGATCGCT GCAGCAGTCG CAGCAGCAAC AGCAGCAGCA GCAACAACAG 
CAGCAGCAAC AACAGATGCA CATGGCGGCC GCGTCGCAAC AATTTTTGGC CGCCCAGCAG 
CAGGCGCAAA ATGCGGCCTA TGCCGCCCAA CAGGCCACGT CCTACGTCAT CAATCCGGGC 
CAGGAGGCTG CCCCGTATAT GGGCATGATT GCCGCCGCCC AGATGCCGTA CTATGGCGTA 
GCACCATGGG GCATGTATCC GGGCAATCTG ATTCCGCAAC AGGGAACGCA GCCGCGCCGC 
CCCCTCACCC CCTCGCAGCA GGGTGCCGAG AATCAGCCGT ATCAGGTCAT CCCGGCATTC 
CTCGATCACA CGGGCTCCTT GCTGATGGGA GGACCTCGCA CCGGGACGCC GATGCGTCTG 
GTTAGCCCCG CCCCCGTTCT GGTGCCCCCG GGCGCTACCC GTGCCGGCCC CCCGCCCCCG 
CAGGGGCCAC AGCTGTATCA GCCGCAGCCG CAGACGGCCC AACAGAATCT CTACTCGCAG 
CAGAATGGAT CCAGTGTCGG AGGCCTCGCC TTGAACACGA GCTCGTTGAC GGGTCGCCGC 
GACTCCTTCG ACCGCAGCAC CTCCGCCTTC AGTCCCTCGA CCATGGACTA CACCAGCAGC 
GGTGTGGCAG CGGCCGCCAA TGCGGTGAAC AGCACAGTGG CCCAGGCAGC AGCAGCTGCC 
GCAGCAGCCG CCGCAGCGCG TGGCAAGTGG CCGGGAGCGA TGTCGGGAGC GGCCAGTGGA 
GCCTACGGAG CCCTGGGAGC GGGCAATGCT TCGGCCAGTC CCCTGGGCGC ACCAATCACG 
CCGCCGCCAT CGGCGCAATC CTGTCTCCTG GGCAGTCGGG CACCTGGAGC CGAGTCCCGC 
CAGCGGCAGC AGCAACAACA GCAGCTGGCC GCCGTTGGTC TGCCGGCGAC TGCAGCAGCT 
GCTCAGGCAG CGGTGGCCGC GGCTGCCAAC AATATGTTCG GATCCAACAG CTCGATCTTC 
TCGAATCCCC TGGCCATTCC GGGTACCGCA GCTGTGGCAG CTGCAGCGGC AGCAGCAGCG 
GCCGCCAACT CGCGTCAGGT GGCTGCCACG GCAGCGGCAG CAGCGGCGGT GGCAGCAGCA 
GCCGGCGGAG TGGGAGGTGC CCCACAGCCA GGAAGATCTC GCCTTCTCGA AGATTTCCGC 
AACCAGCGGT ATCCAAATCT TCAGCTACGC GATCTCGCTA ACCACATTGT GGAGTTCTCA 
CAGGATCAGC ACGGCTCGCG GTTTATCCAA CAGAAGTTGG AGCGGGCCAC CGCCGCCGAG 
AAGCAAATGG TGTTCAGCGA GATCCTGGCG GCAGCCTATA GCCTGATGAC CGATGTCTTT 
GGCAACTATG TCATCCAGAA GTTCTTTGAG TTCGGCACTC CCGAGCAGAA GAACACGCTG 
GGCATGCAGG TCAAGGGTCA TGTGCTGCAG CTGGCGCTGC AAATGTATGG CTGCCGAGTG 
ATTCAGAAGG CTCTGGAGAG CATCTCGCCG GAGCAGCAGC AGGAAATCGT CCACGAACTG 
GACGGACATG TGCTGAAATG CGTCAAGGAT CAGAATGGCA ATCATGTGGT GCAAAAGTGC 
ATTGAGTGCG TGGACCCCGT GGCGCTGCAG TTCATCATCA ATGCTTTCAA GGGTCAGGTT 
TACTCGCTAA GCACCCATCC GTATGGATGC CGGGTGATCC AGAGAATCCT TGAGCATTGC 
ACTGCCGAAC AGACCACGCC CATTTTGGAC GAACTGCATG AGCACACCGA ACAGTTGATT 
CAGGACCAAT ATGGCAACTA TGTTATTCAG CATGTGCTTG AACACGGCAA GCAGGAGGAT 
AAGTCGATTC TTATCAACAG CGTGCGCGGC AAAGTTCTGG TGCTATCACA GCACAAGTTC 
GCCTCAAACG TTGTGGAGAA ATGTGTTACC CATGCCACTC GCGGAGAACG CACTGGTCTC 
ATAGACGAGG TCTGCACCTT CAACGACAAC GCGTTGCACG TGATGATGAA GGATCAGTAT 
GCCAACTATG TGGTCCAAAA AATGATCGAT GTATCGGAGC CGACGCAGCT CAAGAAGCTG 
ATGACCAAGA TCCGGCCCCA CATGGCCGCC TTGCGCAAGT ACACCTACGG CAAGCACATC 
AATGCCAAGT TGGAGAAGTA CTACATGAAG ATAACCAATC CCATTACGGT GGGCACAGGA 
GCTGGAGGAG TGCCGGCAGC CTCGTCGGCG GCGGCAGTCA GCAGTGGTGC CACCTCGGCA 
TCGGTAACCG CCTGCACCAG TGGCAGCAGC ACCACCACGA CCAGCACTAC CAACAGCCTG 
GCCTCACCCA CCATTTGTTC GGTGCAGGAG AACGGCAGCG CCATGGTTGT GGAGCCCTCC 
TCCCCGGACG CCTCCGAGTC CTCGTCCTCG GTGGTGTCAG GCGCTGTCAA CAGCAGCTTG 
GGTCCCATTG GACCCCCGAC CAACGGCAAC GTTGTGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum ( NM_169258.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731314.1 Drosophila melanogaster pumilio (pum), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04317
Clone ID Related Accession (Same CDS sequence)	NM_001275474.1 , NM_169258.3 , NM_169259.3 , NM_079561.4
Accession Version	NM_169258.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pumilio, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169258.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTTT TGGGTGGTAA CGATGATCGC AATGGCCGCG GAGGCGTCGG CGTTGGCACG 
GATGCCATTG TAGGATCTCG AGGTGGCGTC TCTCAGGATG CCGCCGATGC AGCTGGTGCC 
GCCGCAGCCG CCGCCGTCGG CTATGTCTTC CAGCAGCGTC CATCGCCTGG TGGGGTTGGC 
GTCGGCGTGG GCGGAGTGGG TGGCGGTGTG CCAGGGGTCG GAGCCGTAGG CTCGACCTTG 
CACGAGGCCG CCGCCGCCGA GTACGCCGCC CACTTTGCCC AGAAGCAACA GCAGACCCGA 
TGGGCGTGCG GCGACGACGG CCATGGGATC GATAACCCGG ACAAATGGAA GTACAATCCG 
CCGATGAATC CGGCCAATGC CGCTCCTGGC GGTCCACCGG GAAATGGCAG TAATGGTGGG 
CCCGGCGCCA TTGGAACCAT TGGCATGGGC AGCGGATTGG GTGGTGGTGG CGGCGGCGGA 
GCTGGCGGCG GAAATAATGG CGGCTCTGGT ACGAATGGCG GTCTGCATCA TCAATCGATG 
GCCGCTGCAG CTGCGAATAT GGCAGCCATG CAACAGGCGG CGGCGTTGGC CAAGCACAAT 
CACATGATAT CACAGGCAGC AGCCGCAGTT GCAGCCCAGC AACAACATCA GCATCCACAC 
CAGCAGCATC CCCAGCAGCA GCAGCAACAG CAGCAGGCGC AGAACCAGGG GCATCCACAT 
CACCTTATGG GCGGTGGCAA TGGACTGGGC AACGGCAATG GATTGGGCAT ACAACATCCC 
GGCCAGCAAC AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACATCCCGG CCAGTACAAC 
GCGAATCTGC TTAACCATGC GGCTGCCTTG GGTCACATGT CATCTTATGC CCAATCGGGT 
GGCAGCATGT ACGACCATCA TGGTGGAGCC ATGCACCCGG GAATGAACGG CGGCATGCCC 
AAGCAACAGC CATTGGGTCC ACCCGGAGCC GGAGGACCCC AGGACTATGT CTACATGGGT 
GGCCAGACCA CTGTGCCCAT GGGAGCCGCA ATGATGCCGC CACAGAATCA ATATATGAAC 
AGCTCTGCTG TTGCAGCTGC CAATCGGAAT GCAGCGATTA CCACATCCAC TGCCAAGAAA 
TTGTGGGAGA AATCCGATGG CAAGGGCGTA TCCTCGAGCA CTCCCGGTGG ACCGTTGCAT 
CCCCTGCAGA TCCCCGGCAT CGGGGATCCC TCCTCCGTGT GGAAGGATCA CACCTGGTCC 
ACACAGGGCG AGAATATATT GGTGCCGCCC CCCTCGCGAG CCTACGCCCA TGGAGGCGCC 
TCCGATACTT CAAACAGCGG CAATGCGGGC ATACTGAGTC CCCGCGATTC GACTTGCGCC 
AAAGTGGTTG AATATGTTTT CAGTGGCTCG CCCACCAACA AAGATAGCTC GCTTTCCGGA 
TTGGAACCGC ATTTGCGGAA TCTAAAGTTT GACGACAACG ATAAGTCACG CGACGATAAG 
GAGAAAGCAA ACTCTCCGTT TGACACAAAC GGTTTGAAGA AAGACGATCA GGTCACAAAC 
TCAAATGGTG TTGTCAACGG CATTGACGAT GACAAGGGCT TCAATCGCAC TCCTGGTTCA 
CGTCAACCAT CACCTGCAGA GGAGTCCCAG CCACGTCCCC CCAATCTACT CTTTCCTCCA 
CTGCCCTTCA ATCACATGCT CATGGATCAT GGCCAAGGCA TGGGAGGCGG CTTGGGCGGA 
GTTGTTGGAT CTGGCAACGG AGTCGGCGGT GGCAGCGGCG GAGGCGGGGC AGGCGGCGCT 
TATGCGGCCC ACCAGCAGAT GGCCGCCCAG ATGAGTCAAT TGCAACCGCC GATGATGAAC 
GGCGTTGGCG GCGGAATGCC AATGGCAGCA CAGTCACCAA TGTTGAATCA CCAGGCAGCT 
GGACCCAATC ACATGGAATC TCCCGGAAAT CTCTTGCAGC AGCAAAATTT TGATGTTCAG 
CAACTGTTTC GCTCGCAGAA TCCGGGCCTA GCAGCAGTTG CCACAAATGC AGCGGCCGCA 
GCAGCAGCCG CAGCAGCTGC CACATCGGCA GCGAGTGCTG CGGCAGCGGT GGGCGCACCA 
CCCGTTCCCA ACGGATCGCT GCAGCAGTCG CAGCAGCAAC AGCAGCAGCA GCAACAACAG 
CAGCAGCAAC AACAGATGCA CATGGCGGCC GCGTCGCAAC AATTTTTGGC CGCCCAGCAG 
CAGGCGCAAA ATGCGGCCTA TGCCGCCCAA CAGGCCACGT CCTACGTCAT CAATCCGGGC 
CAGGAGGCTG CCCCGTATAT GGGCATGATT GCCGCCGCCC AGATGCCGTA CTATGGCGTA 
GCACCATGGG GCATGTATCC GGGCAATCTG ATTCCGCAAC AGGGAACGCA GCCGCGCCGC 
CCCCTCACCC CCTCGCAGCA GGGTGCCGAG AATCAGCCGT ATCAGGTCAT CCCGGCATTC 
CTCGATCACA CGGGCTCCTT GCTGATGGGA GGACCTCGCA CCGGGACGCC GATGCGTCTG 
GTTAGCCCCG CCCCCGTTCT GGTGCCCCCG GGCGCTACCC GTGCCGGCCC CCCGCCCCCG 
CAGGGGCCAC AGCTGTATCA GCCGCAGCCG CAGACGGCCC AACAGAATCT CTACTCGCAG 
CAGAATGGAT CCAGTGTCGG AGGCCTCGCC TTGAACACGA GCTCGTTGAC GGGTCGCCGC 
GACTCCTTCG ACCGCAGCAC CTCCGCCTTC AGTCCCTCGA CCATGGACTA CACCAGCAGC 
GGTGTGGCAG CGGCCGCCAA TGCGGTGAAC AGCACAGTGG CCCAGGCAGC AGCAGCTGCC 
GCAGCAGCCG CCGCAGCGCG TGGCAAGTGG CCGGGAGCGA TGTCGGGAGC GGCCAGTGGA 
GCCTACGGAG CCCTGGGAGC GGGCAATGCT TCGGCCAGTC CCCTGGGCGC ACCAATCACG 
CCGCCGCCAT CGGCGCAATC CTGTCTCCTG GGCAGTCGGG CACCTGGAGC CGAGTCCCGC 
CAGCGGCAGC AGCAACAACA GCAGCTGGCC GCCGTTGGTC TGCCGGCGAC TGCAGCAGCT 
GCTCAGGCAG CGGTGGCCGC GGCTGCCAAC AATATGTTCG GATCCAACAG CTCGATCTTC 
TCGAATCCCC TGGCCATTCC GGGTACCGCA GCTGTGGCAG CTGCAGCGGC AGCAGCAGCG 
GCCGCCAACT CGCGTCAGGT GGCTGCCACG GCAGCGGCAG CAGCGGCGGT GGCAGCAGCA 
GCCGGCGGAG TGGGAGGTGC CCCACAGCCA GGAAGATCTC GCCTTCTCGA AGATTTCCGC 
AACCAGCGGT ATCCAAATCT TCAGCTACGC GATCTCGCTA ACCACATTGT GGAGTTCTCA 
CAGGATCAGC ACGGCTCGCG GTTTATCCAA CAGAAGTTGG AGCGGGCCAC CGCCGCCGAG 
AAGCAAATGG TGTTCAGCGA GATCCTGGCG GCAGCCTATA GCCTGATGAC CGATGTCTTT 
GGCAACTATG TCATCCAGAA GTTCTTTGAG TTCGGCACTC CCGAGCAGAA GAACACGCTG 
GGCATGCAGG TCAAGGGTCA TGTGCTGCAG CTGGCGCTGC AAATGTATGG CTGCCGAGTG 
ATTCAGAAGG CTCTGGAGAG CATCTCGCCG GAGCAGCAGC AGGAAATCGT CCACGAACTG 
GACGGACATG TGCTGAAATG CGTCAAGGAT CAGAATGGCA ATCATGTGGT GCAAAAGTGC 
ATTGAGTGCG TGGACCCCGT GGCGCTGCAG TTCATCATCA ATGCTTTCAA GGGTCAGGTT 
TACTCGCTAA GCACCCATCC GTATGGATGC CGGGTGATCC AGAGAATCCT TGAGCATTGC 
ACTGCCGAAC AGACCACGCC CATTTTGGAC GAACTGCATG AGCACACCGA ACAGTTGATT 
CAGGACCAAT ATGGCAACTA TGTTATTCAG CATGTGCTTG AACACGGCAA GCAGGAGGAT 
AAGTCGATTC TTATCAACAG CGTGCGCGGC AAAGTTCTGG TGCTATCACA GCACAAGTTC 
GCCTCAAACG TTGTGGAGAA ATGTGTTACC CATGCCACTC GCGGAGAACG CACTGGTCTC 
ATAGACGAGG TCTGCACCTT CAACGACAAC GCGTTGCACG TGATGATGAA GGATCAGTAT 
GCCAACTATG TGGTCCAAAA AATGATCGAT GTATCGGAGC CGACGCAGCT CAAGAAGCTG 
ATGACCAAGA TCCGGCCCCA CATGGCCGCC TTGCGCAAGT ACACCTACGG CAAGCACATC 
AATGCCAAGT TGGAGAAGTA CTACATGAAG ATAACCAATC CCATTACGGT GGGCACAGGA 
GCTGGAGGAG TGCCGGCAGC CTCGTCGGCG GCGGCAGTCA GCAGTGGTGC CACCTCGGCA 
TCGGTAACCG CCTGCACCAG TGGCAGCAGC ACCACCACGA CCAGCACTAC CAACAGCCTG 
GCCTCACCCA CCATTTGTTC GGTGCAGGAG AACGGCAGCG CCATGGTTGT GGAGCCCTCC 
TCCCCGGACG CCTCCGAGTC CTCGTCCTCG GTGGTGTCAG GCGCTGTCAA CAGCAGCTTG 
GGTCCCATTG GACCCCCGAC CAACGGCAAC GTTGTGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731314.1
CDS	872..5473
Translation	MKFLGGNDDRNGRGGVGVGTDAIVGSRGGVSQDAADAAGAAAAAAVGYVFQQRPSPGGVGVGVGGVGGGVPGVGAVGSTLHEAAAAEYAAHFAQKQQQTRWACGDDGHGIDNPDKWKYNPPMNPANAAPGGPPGNGSNGGPGAIGTIGMGSGLGGGGGGGAGGGNNGGSGTNGGLHHQSMAAAAANMAAMQQAAALAKHNHMISQAAAAVAAQQQHQHPHQQHPQQQQQQQQAQNQGHPHHLMGGGNGLGNGNGLGIQHPGQQQQQQQQQQQQQHPGQYNANLLNHAAALGHMSSYAQSGGSMYDHHGGAMHPGMNGGMPKQQPLGPPGAGGPQDYVYMGGQTTVPMGAAMMPPQNQYMNSSAVAAANRNAAITTSTAKKLWEKSDGKGVSSSTPGGPLHPLQIPGIGDPSSVWKDHTWSTQGENILVPPPSRAYAHGGASDTSNSGNAGILSPRDSTCAKVVEYVFSGSPTNKDSSLSGLEPHLRNLKFDDNDKSRDDKEKANSPFDTNGLKKDDQVTNSNGVVNGIDDDKGFNRTPGSRQPSPAEESQPRPPNLLFPPLPFNHMLMDHGQGMGGGLGGVVGSGNGVGGGSGGGGAGGAYAAHQQMAAQMSQLQPPMMNGVGGGMPMAAQSPMLNHQAAGPNHMESPGNLLQQQNFDVQQLFRSQNPGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQQQQQQMHMAAASQQFLAAQQQAQNAAYAAQQATSYVINPGQEAAPYMGMIAAAQMPYYGVAPWGMYPGNLIPQQGTQPRRPLTPSQQGAENQPYQVIPAFLDHTGSLLMGGPRTGTPMRLVSPAPVLVPPGATRAGPPPPQGPQLYQPQPQTAQQNLYSQQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPSTMDYTSSGVAAAANAVNSTVAQAAAAAAAAAAARGKWPGAMSGAASGAYGALGAGNASASPLGAPITPPPSAQSCLLGSRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCTFNDNALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLEKYYMKITNPITVGTGAGGVPAASSAAAVSSGATSASVTACTSGSSTTTTSTTNSLASPTICSVQENGSAMVVEPSSPDASESSSSVVSGAVNSSLGPIGPPTNGNVVL

Target ORF information:

RefSeq Version	NM_169258.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pumilio (pum), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169258.3

ORF Insert Sequence:

ATGAAGTTTT TGGGTGGTAA CGATGATCGC AATGGCCGCG GAGGCGTCGG CGTTGGCACG 
GATGCCATTG TAGGATCTCG AGGTGGCGTC TCTCAGGATG CCGCCGATGC AGCTGGTGCC 
GCCGCAGCCG CCGCCGTCGG CTATGTCTTC CAGCAGCGTC CATCGCCTGG TGGGGTTGGC 
GTCGGCGTGG GCGGAGTGGG TGGCGGTGTG CCAGGGGTCG GAGCCGTAGG CTCGACCTTG 
CACGAGGCCG CCGCCGCCGA GTACGCCGCC CACTTTGCCC AGAAGCAACA GCAGACCCGA 
TGGGCGTGCG GCGACGACGG CCATGGGATC GATAACCCGG ACAAATGGAA GTACAATCCG 
CCGATGAATC CGGCCAATGC CGCTCCTGGC GGTCCACCGG GAAATGGCAG TAATGGTGGG 
CCCGGCGCCA TTGGAACCAT TGGCATGGGC AGCGGATTGG GTGGTGGTGG CGGCGGCGGA 
GCTGGCGGCG GAAATAATGG CGGCTCTGGT ACGAATGGCG GTCTGCATCA TCAATCGATG 
GCCGCTGCAG CTGCGAATAT GGCAGCCATG CAACAGGCGG CGGCGTTGGC CAAGCACAAT 
CACATGATAT CACAGGCAGC AGCCGCAGTT GCAGCCCAGC AACAACATCA GCATCCACAC 
CAGCAGCATC CCCAGCAGCA GCAGCAACAG CAGCAGGCGC AGAACCAGGG GCATCCACAT 
CACCTTATGG GCGGTGGCAA TGGACTGGGC AACGGCAATG GATTGGGCAT ACAACATCCC 
GGCCAGCAAC AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACATCCCGG CCAGTACAAC 
GCGAATCTGC TTAACCATGC GGCTGCCTTG GGTCACATGT CATCTTATGC CCAATCGGGT 
GGCAGCATGT ACGACCATCA TGGTGGAGCC ATGCACCCGG GAATGAACGG CGGCATGCCC 
AAGCAACAGC CATTGGGTCC ACCCGGAGCC GGAGGACCCC AGGACTATGT CTACATGGGT 
GGCCAGACCA CTGTGCCCAT GGGAGCCGCA ATGATGCCGC CACAGAATCA ATATATGAAC 
AGCTCTGCTG TTGCAGCTGC CAATCGGAAT GCAGCGATTA CCACATCCAC TGCCAAGAAA 
TTGTGGGAGA AATCCGATGG CAAGGGCGTA TCCTCGAGCA CTCCCGGTGG ACCGTTGCAT 
CCCCTGCAGA TCCCCGGCAT CGGGGATCCC TCCTCCGTGT GGAAGGATCA CACCTGGTCC 
ACACAGGGCG AGAATATATT GGTGCCGCCC CCCTCGCGAG CCTACGCCCA TGGAGGCGCC 
TCCGATACTT CAAACAGCGG CAATGCGGGC ATACTGAGTC CCCGCGATTC GACTTGCGCC 
AAAGTGGTTG AATATGTTTT CAGTGGCTCG CCCACCAACA AAGATAGCTC GCTTTCCGGA 
TTGGAACCGC ATTTGCGGAA TCTAAAGTTT GACGACAACG ATAAGTCACG CGACGATAAG 
GAGAAAGCAA ACTCTCCGTT TGACACAAAC GGTTTGAAGA AAGACGATCA GGTCACAAAC 
TCAAATGGTG TTGTCAACGG CATTGACGAT GACAAGGGCT TCAATCGCAC TCCTGGTTCA 
CGTCAACCAT CACCTGCAGA GGAGTCCCAG CCACGTCCCC CCAATCTACT CTTTCCTCCA 
CTGCCCTTCA ATCACATGCT CATGGATCAT GGCCAAGGCA TGGGAGGCGG CTTGGGCGGA 
GTTGTTGGAT CTGGCAACGG AGTCGGCGGT GGCAGCGGCG GAGGCGGGGC AGGCGGCGCT 
TATGCGGCCC ACCAGCAGAT GGCCGCCCAG ATGAGTCAAT TGCAACCGCC GATGATGAAC 
GGCGTTGGCG GCGGAATGCC AATGGCAGCA CAGTCACCAA TGTTGAATCA CCAGGCAGCT 
GGACCCAATC ACATGGAATC TCCCGGAAAT CTCTTGCAGC AGCAAAATTT TGATGTTCAG 
CAACTGTTTC GCTCGCAGAA TCCGGGCCTA GCAGCAGTTG CCACAAATGC AGCGGCCGCA 
GCAGCAGCCG CAGCAGCTGC CACATCGGCA GCGAGTGCTG CGGCAGCGGT GGGCGCACCA 
CCCGTTCCCA ACGGATCGCT GCAGCAGTCG CAGCAGCAAC AGCAGCAGCA GCAACAACAG 
CAGCAGCAAC AACAGATGCA CATGGCGGCC GCGTCGCAAC AATTTTTGGC CGCCCAGCAG 
CAGGCGCAAA ATGCGGCCTA TGCCGCCCAA CAGGCCACGT CCTACGTCAT CAATCCGGGC 
CAGGAGGCTG CCCCGTATAT GGGCATGATT GCCGCCGCCC AGATGCCGTA CTATGGCGTA 
GCACCATGGG GCATGTATCC GGGCAATCTG ATTCCGCAAC AGGGAACGCA GCCGCGCCGC 
CCCCTCACCC CCTCGCAGCA GGGTGCCGAG AATCAGCCGT ATCAGGTCAT CCCGGCATTC 
CTCGATCACA CGGGCTCCTT GCTGATGGGA GGACCTCGCA CCGGGACGCC GATGCGTCTG 
GTTAGCCCCG CCCCCGTTCT GGTGCCCCCG GGCGCTACCC GTGCCGGCCC CCCGCCCCCG 
CAGGGGCCAC AGCTGTATCA GCCGCAGCCG CAGACGGCCC AACAGAATCT CTACTCGCAG 
CAGAATGGAT CCAGTGTCGG AGGCCTCGCC TTGAACACGA GCTCGTTGAC GGGTCGCCGC 
GACTCCTTCG ACCGCAGCAC CTCCGCCTTC AGTCCCTCGA CCATGGACTA CACCAGCAGC 
GGTGTGGCAG CGGCCGCCAA TGCGGTGAAC AGCACAGTGG CCCAGGCAGC AGCAGCTGCC 
GCAGCAGCCG CCGCAGCGCG TGGCAAGTGG CCGGGAGCGA TGTCGGGAGC GGCCAGTGGA 
GCCTACGGAG CCCTGGGAGC GGGCAATGCT TCGGCCAGTC CCCTGGGCGC ACCAATCACG 
CCGCCGCCAT CGGCGCAATC CTGTCTCCTG GGCAGTCGGG CACCTGGAGC CGAGTCCCGC 
CAGCGGCAGC AGCAACAACA GCAGCTGGCC GCCGTTGGTC TGCCGGCGAC TGCAGCAGCT 
GCTCAGGCAG CGGTGGCCGC GGCTGCCAAC AATATGTTCG GATCCAACAG CTCGATCTTC 
TCGAATCCCC TGGCCATTCC GGGTACCGCA GCTGTGGCAG CTGCAGCGGC AGCAGCAGCG 
GCCGCCAACT CGCGTCAGGT GGCTGCCACG GCAGCGGCAG CAGCGGCGGT GGCAGCAGCA 
GCCGGCGGAG TGGGAGGTGC CCCACAGCCA GGAAGATCTC GCCTTCTCGA AGATTTCCGC 
AACCAGCGGT ATCCAAATCT TCAGCTACGC GATCTCGCTA ACCACATTGT GGAGTTCTCA 
CAGGATCAGC ACGGCTCGCG GTTTATCCAA CAGAAGTTGG AGCGGGCCAC CGCCGCCGAG 
AAGCAAATGG TGTTCAGCGA GATCCTGGCG GCAGCCTATA GCCTGATGAC CGATGTCTTT 
GGCAACTATG TCATCCAGAA GTTCTTTGAG TTCGGCACTC CCGAGCAGAA GAACACGCTG 
GGCATGCAGG TCAAGGGTCA TGTGCTGCAG CTGGCGCTGC AAATGTATGG CTGCCGAGTG 
ATTCAGAAGG CTCTGGAGAG CATCTCGCCG GAGCAGCAGC AGGAAATCGT CCACGAACTG 
GACGGACATG TGCTGAAATG CGTCAAGGAT CAGAATGGCA ATCATGTGGT GCAAAAGTGC 
ATTGAGTGCG TGGACCCCGT GGCGCTGCAG TTCATCATCA ATGCTTTCAA GGGTCAGGTT 
TACTCGCTAA GCACCCATCC GTATGGATGC CGGGTGATCC AGAGAATCCT TGAGCATTGC 
ACTGCCGAAC AGACCACGCC CATTTTGGAC GAACTGCATG AGCACACCGA ACAGTTGATT 
CAGGACCAAT ATGGCAACTA TGTTATTCAG CATGTGCTTG AACACGGCAA GCAGGAGGAT 
AAGTCGATTC TTATCAACAG CGTGCGCGGC AAAGTTCTGG TGCTATCACA GCACAAGTTC 
GCCTCAAACG TTGTGGAGAA ATGTGTTACC CATGCCACTC GCGGAGAACG CACTGGTCTC 
ATAGACGAGG TCTGCACCTT CAACGACAAC GCGTTGCACG TGATGATGAA GGATCAGTAT 
GCCAACTATG TGGTCCAAAA AATGATCGAT GTATCGGAGC CGACGCAGCT CAAGAAGCTG 
ATGACCAAGA TCCGGCCCCA CATGGCCGCC TTGCGCAAGT ACACCTACGG CAAGCACATC 
AATGCCAAGT TGGAGAAGTA CTACATGAAG ATAACCAATC CCATTACGGT GGGCACAGGA 
GCTGGAGGAG TGCCGGCAGC CTCGTCGGCG GCGGCAGTCA GCAGTGGTGC CACCTCGGCA 
TCGGTAACCG CCTGCACCAG TGGCAGCAGC ACCACCACGA CCAGCACTAC CAACAGCCTG 
GCCTCACCCA CCATTTGTTC GGTGCAGGAG AACGGCAGCG CCATGGTTGT GGAGCCCTCC 
TCCCCGGACG CCTCCGAGTC CTCGTCCTCG GTGGTGTCAG GCGCTGTCAA CAGCAGCTTG 
GGTCCCATTG GACCCCCGAC CAACGGCAAC GTTGTGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum ( NM_169259.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731315.1 Drosophila melanogaster pumilio (pum), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa04317
Clone ID Related Accession (Same CDS sequence)	NM_001275474.1 , NM_169258.3 , NM_169259.3 , NM_079561.4
Accession Version	NM_169259.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pumilio, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169259.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTTT TGGGTGGTAA CGATGATCGC AATGGCCGCG GAGGCGTCGG CGTTGGCACG 
GATGCCATTG TAGGATCTCG AGGTGGCGTC TCTCAGGATG CCGCCGATGC AGCTGGTGCC 
GCCGCAGCCG CCGCCGTCGG CTATGTCTTC CAGCAGCGTC CATCGCCTGG TGGGGTTGGC 
GTCGGCGTGG GCGGAGTGGG TGGCGGTGTG CCAGGGGTCG GAGCCGTAGG CTCGACCTTG 
CACGAGGCCG CCGCCGCCGA GTACGCCGCC CACTTTGCCC AGAAGCAACA GCAGACCCGA 
TGGGCGTGCG GCGACGACGG CCATGGGATC GATAACCCGG ACAAATGGAA GTACAATCCG 
CCGATGAATC CGGCCAATGC CGCTCCTGGC GGTCCACCGG GAAATGGCAG TAATGGTGGG 
CCCGGCGCCA TTGGAACCAT TGGCATGGGC AGCGGATTGG GTGGTGGTGG CGGCGGCGGA 
GCTGGCGGCG GAAATAATGG CGGCTCTGGT ACGAATGGCG GTCTGCATCA TCAATCGATG 
GCCGCTGCAG CTGCGAATAT GGCAGCCATG CAACAGGCGG CGGCGTTGGC CAAGCACAAT 
CACATGATAT CACAGGCAGC AGCCGCAGTT GCAGCCCAGC AACAACATCA GCATCCACAC 
CAGCAGCATC CCCAGCAGCA GCAGCAACAG CAGCAGGCGC AGAACCAGGG GCATCCACAT 
CACCTTATGG GCGGTGGCAA TGGACTGGGC AACGGCAATG GATTGGGCAT ACAACATCCC 
GGCCAGCAAC AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACATCCCGG CCAGTACAAC 
GCGAATCTGC TTAACCATGC GGCTGCCTTG GGTCACATGT CATCTTATGC CCAATCGGGT 
GGCAGCATGT ACGACCATCA TGGTGGAGCC ATGCACCCGG GAATGAACGG CGGCATGCCC 
AAGCAACAGC CATTGGGTCC ACCCGGAGCC GGAGGACCCC AGGACTATGT CTACATGGGT 
GGCCAGACCA CTGTGCCCAT GGGAGCCGCA ATGATGCCGC CACAGAATCA ATATATGAAC 
AGCTCTGCTG TTGCAGCTGC CAATCGGAAT GCAGCGATTA CCACATCCAC TGCCAAGAAA 
TTGTGGGAGA AATCCGATGG CAAGGGCGTA TCCTCGAGCA CTCCCGGTGG ACCGTTGCAT 
CCCCTGCAGA TCCCCGGCAT CGGGGATCCC TCCTCCGTGT GGAAGGATCA CACCTGGTCC 
ACACAGGGCG AGAATATATT GGTGCCGCCC CCCTCGCGAG CCTACGCCCA TGGAGGCGCC 
TCCGATACTT CAAACAGCGG CAATGCGGGC ATACTGAGTC CCCGCGATTC GACTTGCGCC 
AAAGTGGTTG AATATGTTTT CAGTGGCTCG CCCACCAACA AAGATAGCTC GCTTTCCGGA 
TTGGAACCGC ATTTGCGGAA TCTAAAGTTT GACGACAACG ATAAGTCACG CGACGATAAG 
GAGAAAGCAA ACTCTCCGTT TGACACAAAC GGTTTGAAGA AAGACGATCA GGTCACAAAC 
TCAAATGGTG TTGTCAACGG CATTGACGAT GACAAGGGCT TCAATCGCAC TCCTGGTTCA 
CGTCAACCAT CACCTGCAGA GGAGTCCCAG CCACGTCCCC CCAATCTACT CTTTCCTCCA 
CTGCCCTTCA ATCACATGCT CATGGATCAT GGCCAAGGCA TGGGAGGCGG CTTGGGCGGA 
GTTGTTGGAT CTGGCAACGG AGTCGGCGGT GGCAGCGGCG GAGGCGGGGC AGGCGGCGCT 
TATGCGGCCC ACCAGCAGAT GGCCGCCCAG ATGAGTCAAT TGCAACCGCC GATGATGAAC 
GGCGTTGGCG GCGGAATGCC AATGGCAGCA CAGTCACCAA TGTTGAATCA CCAGGCAGCT 
GGACCCAATC ACATGGAATC TCCCGGAAAT CTCTTGCAGC AGCAAAATTT TGATGTTCAG 
CAACTGTTTC GCTCGCAGAA TCCGGGCCTA GCAGCAGTTG CCACAAATGC AGCGGCCGCA 
GCAGCAGCCG CAGCAGCTGC CACATCGGCA GCGAGTGCTG CGGCAGCGGT GGGCGCACCA 
CCCGTTCCCA ACGGATCGCT GCAGCAGTCG CAGCAGCAAC AGCAGCAGCA GCAACAACAG 
CAGCAGCAAC AACAGATGCA CATGGCGGCC GCGTCGCAAC AATTTTTGGC CGCCCAGCAG 
CAGGCGCAAA ATGCGGCCTA TGCCGCCCAA CAGGCCACGT CCTACGTCAT CAATCCGGGC 
CAGGAGGCTG CCCCGTATAT GGGCATGATT GCCGCCGCCC AGATGCCGTA CTATGGCGTA 
GCACCATGGG GCATGTATCC GGGCAATCTG ATTCCGCAAC AGGGAACGCA GCCGCGCCGC 
CCCCTCACCC CCTCGCAGCA GGGTGCCGAG AATCAGCCGT ATCAGGTCAT CCCGGCATTC 
CTCGATCACA CGGGCTCCTT GCTGATGGGA GGACCTCGCA CCGGGACGCC GATGCGTCTG 
GTTAGCCCCG CCCCCGTTCT GGTGCCCCCG GGCGCTACCC GTGCCGGCCC CCCGCCCCCG 
CAGGGGCCAC AGCTGTATCA GCCGCAGCCG CAGACGGCCC AACAGAATCT CTACTCGCAG 
CAGAATGGAT CCAGTGTCGG AGGCCTCGCC TTGAACACGA GCTCGTTGAC GGGTCGCCGC 
GACTCCTTCG ACCGCAGCAC CTCCGCCTTC AGTCCCTCGA CCATGGACTA CACCAGCAGC 
GGTGTGGCAG CGGCCGCCAA TGCGGTGAAC AGCACAGTGG CCCAGGCAGC AGCAGCTGCC 
GCAGCAGCCG CCGCAGCGCG TGGCAAGTGG CCGGGAGCGA TGTCGGGAGC GGCCAGTGGA 
GCCTACGGAG CCCTGGGAGC GGGCAATGCT TCGGCCAGTC CCCTGGGCGC ACCAATCACG 
CCGCCGCCAT CGGCGCAATC CTGTCTCCTG GGCAGTCGGG CACCTGGAGC CGAGTCCCGC 
CAGCGGCAGC AGCAACAACA GCAGCTGGCC GCCGTTGGTC TGCCGGCGAC TGCAGCAGCT 
GCTCAGGCAG CGGTGGCCGC GGCTGCCAAC AATATGTTCG GATCCAACAG CTCGATCTTC 
TCGAATCCCC TGGCCATTCC GGGTACCGCA GCTGTGGCAG CTGCAGCGGC AGCAGCAGCG 
GCCGCCAACT CGCGTCAGGT GGCTGCCACG GCAGCGGCAG CAGCGGCGGT GGCAGCAGCA 
GCCGGCGGAG TGGGAGGTGC CCCACAGCCA GGAAGATCTC GCCTTCTCGA AGATTTCCGC 
AACCAGCGGT ATCCAAATCT TCAGCTACGC GATCTCGCTA ACCACATTGT GGAGTTCTCA 
CAGGATCAGC ACGGCTCGCG GTTTATCCAA CAGAAGTTGG AGCGGGCCAC CGCCGCCGAG 
AAGCAAATGG TGTTCAGCGA GATCCTGGCG GCAGCCTATA GCCTGATGAC CGATGTCTTT 
GGCAACTATG TCATCCAGAA GTTCTTTGAG TTCGGCACTC CCGAGCAGAA GAACACGCTG 
GGCATGCAGG TCAAGGGTCA TGTGCTGCAG CTGGCGCTGC AAATGTATGG CTGCCGAGTG 
ATTCAGAAGG CTCTGGAGAG CATCTCGCCG GAGCAGCAGC AGGAAATCGT CCACGAACTG 
GACGGACATG TGCTGAAATG CGTCAAGGAT CAGAATGGCA ATCATGTGGT GCAAAAGTGC 
ATTGAGTGCG TGGACCCCGT GGCGCTGCAG TTCATCATCA ATGCTTTCAA GGGTCAGGTT 
TACTCGCTAA GCACCCATCC GTATGGATGC CGGGTGATCC AGAGAATCCT TGAGCATTGC 
ACTGCCGAAC AGACCACGCC CATTTTGGAC GAACTGCATG AGCACACCGA ACAGTTGATT 
CAGGACCAAT ATGGCAACTA TGTTATTCAG CATGTGCTTG AACACGGCAA GCAGGAGGAT 
AAGTCGATTC TTATCAACAG CGTGCGCGGC AAAGTTCTGG TGCTATCACA GCACAAGTTC 
GCCTCAAACG TTGTGGAGAA ATGTGTTACC CATGCCACTC GCGGAGAACG CACTGGTCTC 
ATAGACGAGG TCTGCACCTT CAACGACAAC GCGTTGCACG TGATGATGAA GGATCAGTAT 
GCCAACTATG TGGTCCAAAA AATGATCGAT GTATCGGAGC CGACGCAGCT CAAGAAGCTG 
ATGACCAAGA TCCGGCCCCA CATGGCCGCC TTGCGCAAGT ACACCTACGG CAAGCACATC 
AATGCCAAGT TGGAGAAGTA CTACATGAAG ATAACCAATC CCATTACGGT GGGCACAGGA 
GCTGGAGGAG TGCCGGCAGC CTCGTCGGCG GCGGCAGTCA GCAGTGGTGC CACCTCGGCA 
TCGGTAACCG CCTGCACCAG TGGCAGCAGC ACCACCACGA CCAGCACTAC CAACAGCCTG 
GCCTCACCCA CCATTTGTTC GGTGCAGGAG AACGGCAGCG CCATGGTTGT GGAGCCCTCC 
TCCCCGGACG CCTCCGAGTC CTCGTCCTCG GTGGTGTCAG GCGCTGTCAA CAGCAGCTTG 
GGTCCCATTG GACCCCCGAC CAACGGCAAC GTTGTGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731315.1
CDS	870..5471
Translation	MKFLGGNDDRNGRGGVGVGTDAIVGSRGGVSQDAADAAGAAAAAAVGYVFQQRPSPGGVGVGVGGVGGGVPGVGAVGSTLHEAAAAEYAAHFAQKQQQTRWACGDDGHGIDNPDKWKYNPPMNPANAAPGGPPGNGSNGGPGAIGTIGMGSGLGGGGGGGAGGGNNGGSGTNGGLHHQSMAAAAANMAAMQQAAALAKHNHMISQAAAAVAAQQQHQHPHQQHPQQQQQQQQAQNQGHPHHLMGGGNGLGNGNGLGIQHPGQQQQQQQQQQQQQHPGQYNANLLNHAAALGHMSSYAQSGGSMYDHHGGAMHPGMNGGMPKQQPLGPPGAGGPQDYVYMGGQTTVPMGAAMMPPQNQYMNSSAVAAANRNAAITTSTAKKLWEKSDGKGVSSSTPGGPLHPLQIPGIGDPSSVWKDHTWSTQGENILVPPPSRAYAHGGASDTSNSGNAGILSPRDSTCAKVVEYVFSGSPTNKDSSLSGLEPHLRNLKFDDNDKSRDDKEKANSPFDTNGLKKDDQVTNSNGVVNGIDDDKGFNRTPGSRQPSPAEESQPRPPNLLFPPLPFNHMLMDHGQGMGGGLGGVVGSGNGVGGGSGGGGAGGAYAAHQQMAAQMSQLQPPMMNGVGGGMPMAAQSPMLNHQAAGPNHMESPGNLLQQQNFDVQQLFRSQNPGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQQQQQQMHMAAASQQFLAAQQQAQNAAYAAQQATSYVINPGQEAAPYMGMIAAAQMPYYGVAPWGMYPGNLIPQQGTQPRRPLTPSQQGAENQPYQVIPAFLDHTGSLLMGGPRTGTPMRLVSPAPVLVPPGATRAGPPPPQGPQLYQPQPQTAQQNLYSQQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPSTMDYTSSGVAAAANAVNSTVAQAAAAAAAAAAARGKWPGAMSGAASGAYGALGAGNASASPLGAPITPPPSAQSCLLGSRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCTFNDNALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLEKYYMKITNPITVGTGAGGVPAASSAAAVSSGATSASVTACTSGSSTTTTSTTNSLASPTICSVQENGSAMVVEPSSPDASESSSSVVSGAVNSSLGPIGPPTNGNVVL

Target ORF information:

RefSeq Version	NM_169259.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pumilio (pum), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169259.3

ORF Insert Sequence:

ATGAAGTTTT TGGGTGGTAA CGATGATCGC AATGGCCGCG GAGGCGTCGG CGTTGGCACG 
GATGCCATTG TAGGATCTCG AGGTGGCGTC TCTCAGGATG CCGCCGATGC AGCTGGTGCC 
GCCGCAGCCG CCGCCGTCGG CTATGTCTTC CAGCAGCGTC CATCGCCTGG TGGGGTTGGC 
GTCGGCGTGG GCGGAGTGGG TGGCGGTGTG CCAGGGGTCG GAGCCGTAGG CTCGACCTTG 
CACGAGGCCG CCGCCGCCGA GTACGCCGCC CACTTTGCCC AGAAGCAACA GCAGACCCGA 
TGGGCGTGCG GCGACGACGG CCATGGGATC GATAACCCGG ACAAATGGAA GTACAATCCG 
CCGATGAATC CGGCCAATGC CGCTCCTGGC GGTCCACCGG GAAATGGCAG TAATGGTGGG 
CCCGGCGCCA TTGGAACCAT TGGCATGGGC AGCGGATTGG GTGGTGGTGG CGGCGGCGGA 
GCTGGCGGCG GAAATAATGG CGGCTCTGGT ACGAATGGCG GTCTGCATCA TCAATCGATG 
GCCGCTGCAG CTGCGAATAT GGCAGCCATG CAACAGGCGG CGGCGTTGGC CAAGCACAAT 
CACATGATAT CACAGGCAGC AGCCGCAGTT GCAGCCCAGC AACAACATCA GCATCCACAC 
CAGCAGCATC CCCAGCAGCA GCAGCAACAG CAGCAGGCGC AGAACCAGGG GCATCCACAT 
CACCTTATGG GCGGTGGCAA TGGACTGGGC AACGGCAATG GATTGGGCAT ACAACATCCC 
GGCCAGCAAC AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACATCCCGG CCAGTACAAC 
GCGAATCTGC TTAACCATGC GGCTGCCTTG GGTCACATGT CATCTTATGC CCAATCGGGT 
GGCAGCATGT ACGACCATCA TGGTGGAGCC ATGCACCCGG GAATGAACGG CGGCATGCCC 
AAGCAACAGC CATTGGGTCC ACCCGGAGCC GGAGGACCCC AGGACTATGT CTACATGGGT 
GGCCAGACCA CTGTGCCCAT GGGAGCCGCA ATGATGCCGC CACAGAATCA ATATATGAAC 
AGCTCTGCTG TTGCAGCTGC CAATCGGAAT GCAGCGATTA CCACATCCAC TGCCAAGAAA 
TTGTGGGAGA AATCCGATGG CAAGGGCGTA TCCTCGAGCA CTCCCGGTGG ACCGTTGCAT 
CCCCTGCAGA TCCCCGGCAT CGGGGATCCC TCCTCCGTGT GGAAGGATCA CACCTGGTCC 
ACACAGGGCG AGAATATATT GGTGCCGCCC CCCTCGCGAG CCTACGCCCA TGGAGGCGCC 
TCCGATACTT CAAACAGCGG CAATGCGGGC ATACTGAGTC CCCGCGATTC GACTTGCGCC 
AAAGTGGTTG AATATGTTTT CAGTGGCTCG CCCACCAACA AAGATAGCTC GCTTTCCGGA 
TTGGAACCGC ATTTGCGGAA TCTAAAGTTT GACGACAACG ATAAGTCACG CGACGATAAG 
GAGAAAGCAA ACTCTCCGTT TGACACAAAC GGTTTGAAGA AAGACGATCA GGTCACAAAC 
TCAAATGGTG TTGTCAACGG CATTGACGAT GACAAGGGCT TCAATCGCAC TCCTGGTTCA 
CGTCAACCAT CACCTGCAGA GGAGTCCCAG CCACGTCCCC CCAATCTACT CTTTCCTCCA 
CTGCCCTTCA ATCACATGCT CATGGATCAT GGCCAAGGCA TGGGAGGCGG CTTGGGCGGA 
GTTGTTGGAT CTGGCAACGG AGTCGGCGGT GGCAGCGGCG GAGGCGGGGC AGGCGGCGCT 
TATGCGGCCC ACCAGCAGAT GGCCGCCCAG ATGAGTCAAT TGCAACCGCC GATGATGAAC 
GGCGTTGGCG GCGGAATGCC AATGGCAGCA CAGTCACCAA TGTTGAATCA CCAGGCAGCT 
GGACCCAATC ACATGGAATC TCCCGGAAAT CTCTTGCAGC AGCAAAATTT TGATGTTCAG 
CAACTGTTTC GCTCGCAGAA TCCGGGCCTA GCAGCAGTTG CCACAAATGC AGCGGCCGCA 
GCAGCAGCCG CAGCAGCTGC CACATCGGCA GCGAGTGCTG CGGCAGCGGT GGGCGCACCA 
CCCGTTCCCA ACGGATCGCT GCAGCAGTCG CAGCAGCAAC AGCAGCAGCA GCAACAACAG 
CAGCAGCAAC AACAGATGCA CATGGCGGCC GCGTCGCAAC AATTTTTGGC CGCCCAGCAG 
CAGGCGCAAA ATGCGGCCTA TGCCGCCCAA CAGGCCACGT CCTACGTCAT CAATCCGGGC 
CAGGAGGCTG CCCCGTATAT GGGCATGATT GCCGCCGCCC AGATGCCGTA CTATGGCGTA 
GCACCATGGG GCATGTATCC GGGCAATCTG ATTCCGCAAC AGGGAACGCA GCCGCGCCGC 
CCCCTCACCC CCTCGCAGCA GGGTGCCGAG AATCAGCCGT ATCAGGTCAT CCCGGCATTC 
CTCGATCACA CGGGCTCCTT GCTGATGGGA GGACCTCGCA CCGGGACGCC GATGCGTCTG 
GTTAGCCCCG CCCCCGTTCT GGTGCCCCCG GGCGCTACCC GTGCCGGCCC CCCGCCCCCG 
CAGGGGCCAC AGCTGTATCA GCCGCAGCCG CAGACGGCCC AACAGAATCT CTACTCGCAG 
CAGAATGGAT CCAGTGTCGG AGGCCTCGCC TTGAACACGA GCTCGTTGAC GGGTCGCCGC 
GACTCCTTCG ACCGCAGCAC CTCCGCCTTC AGTCCCTCGA CCATGGACTA CACCAGCAGC 
GGTGTGGCAG CGGCCGCCAA TGCGGTGAAC AGCACAGTGG CCCAGGCAGC AGCAGCTGCC 
GCAGCAGCCG CCGCAGCGCG TGGCAAGTGG CCGGGAGCGA TGTCGGGAGC GGCCAGTGGA 
GCCTACGGAG CCCTGGGAGC GGGCAATGCT TCGGCCAGTC CCCTGGGCGC ACCAATCACG 
CCGCCGCCAT CGGCGCAATC CTGTCTCCTG GGCAGTCGGG CACCTGGAGC CGAGTCCCGC 
CAGCGGCAGC AGCAACAACA GCAGCTGGCC GCCGTTGGTC TGCCGGCGAC TGCAGCAGCT 
GCTCAGGCAG CGGTGGCCGC GGCTGCCAAC AATATGTTCG GATCCAACAG CTCGATCTTC 
TCGAATCCCC TGGCCATTCC GGGTACCGCA GCTGTGGCAG CTGCAGCGGC AGCAGCAGCG 
GCCGCCAACT CGCGTCAGGT GGCTGCCACG GCAGCGGCAG CAGCGGCGGT GGCAGCAGCA 
GCCGGCGGAG TGGGAGGTGC CCCACAGCCA GGAAGATCTC GCCTTCTCGA AGATTTCCGC 
AACCAGCGGT ATCCAAATCT TCAGCTACGC GATCTCGCTA ACCACATTGT GGAGTTCTCA 
CAGGATCAGC ACGGCTCGCG GTTTATCCAA CAGAAGTTGG AGCGGGCCAC CGCCGCCGAG 
AAGCAAATGG TGTTCAGCGA GATCCTGGCG GCAGCCTATA GCCTGATGAC CGATGTCTTT 
GGCAACTATG TCATCCAGAA GTTCTTTGAG TTCGGCACTC CCGAGCAGAA GAACACGCTG 
GGCATGCAGG TCAAGGGTCA TGTGCTGCAG CTGGCGCTGC AAATGTATGG CTGCCGAGTG 
ATTCAGAAGG CTCTGGAGAG CATCTCGCCG GAGCAGCAGC AGGAAATCGT CCACGAACTG 
GACGGACATG TGCTGAAATG CGTCAAGGAT CAGAATGGCA ATCATGTGGT GCAAAAGTGC 
ATTGAGTGCG TGGACCCCGT GGCGCTGCAG TTCATCATCA ATGCTTTCAA GGGTCAGGTT 
TACTCGCTAA GCACCCATCC GTATGGATGC CGGGTGATCC AGAGAATCCT TGAGCATTGC 
ACTGCCGAAC AGACCACGCC CATTTTGGAC GAACTGCATG AGCACACCGA ACAGTTGATT 
CAGGACCAAT ATGGCAACTA TGTTATTCAG CATGTGCTTG AACACGGCAA GCAGGAGGAT 
AAGTCGATTC TTATCAACAG CGTGCGCGGC AAAGTTCTGG TGCTATCACA GCACAAGTTC 
GCCTCAAACG TTGTGGAGAA ATGTGTTACC CATGCCACTC GCGGAGAACG CACTGGTCTC 
ATAGACGAGG TCTGCACCTT CAACGACAAC GCGTTGCACG TGATGATGAA GGATCAGTAT 
GCCAACTATG TGGTCCAAAA AATGATCGAT GTATCGGAGC CGACGCAGCT CAAGAAGCTG 
ATGACCAAGA TCCGGCCCCA CATGGCCGCC TTGCGCAAGT ACACCTACGG CAAGCACATC 
AATGCCAAGT TGGAGAAGTA CTACATGAAG ATAACCAATC CCATTACGGT GGGCACAGGA 
GCTGGAGGAG TGCCGGCAGC CTCGTCGGCG GCGGCAGTCA GCAGTGGTGC CACCTCGGCA 
TCGGTAACCG CCTGCACCAG TGGCAGCAGC ACCACCACGA CCAGCACTAC CAACAGCCTG 
GCCTCACCCA CCATTTGTTC GGTGCAGGAG AACGGCAGCG CCATGGTTGT GGAGCCCTCC 
TCCCCGGACG CCTCCGAGTC CTCGTCCTCG GTGGTGTCAG GCGCTGTCAA CAGCAGCTTG 
GGTCCCATTG GACCCCCGAC CAACGGCAAC GTTGTGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum ( NM_079561.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524285.2 Drosophila melanogaster pumilio (pum), transcript variant C, mRNA.

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CloneID	OFa04317
Clone ID Related Accession (Same CDS sequence)	NM_001275474.1 , NM_169258.3 , NM_169259.3 , NM_079561.4
Accession Version	NM_079561.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pumilio, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079561.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTTT TGGGTGGTAA CGATGATCGC AATGGCCGCG GAGGCGTCGG CGTTGGCACG 
GATGCCATTG TAGGATCTCG AGGTGGCGTC TCTCAGGATG CCGCCGATGC AGCTGGTGCC 
GCCGCAGCCG CCGCCGTCGG CTATGTCTTC CAGCAGCGTC CATCGCCTGG TGGGGTTGGC 
GTCGGCGTGG GCGGAGTGGG TGGCGGTGTG CCAGGGGTCG GAGCCGTAGG CTCGACCTTG 
CACGAGGCCG CCGCCGCCGA GTACGCCGCC CACTTTGCCC AGAAGCAACA GCAGACCCGA 
TGGGCGTGCG GCGACGACGG CCATGGGATC GATAACCCGG ACAAATGGAA GTACAATCCG 
CCGATGAATC CGGCCAATGC CGCTCCTGGC GGTCCACCGG GAAATGGCAG TAATGGTGGG 
CCCGGCGCCA TTGGAACCAT TGGCATGGGC AGCGGATTGG GTGGTGGTGG CGGCGGCGGA 
GCTGGCGGCG GAAATAATGG CGGCTCTGGT ACGAATGGCG GTCTGCATCA TCAATCGATG 
GCCGCTGCAG CTGCGAATAT GGCAGCCATG CAACAGGCGG CGGCGTTGGC CAAGCACAAT 
CACATGATAT CACAGGCAGC AGCCGCAGTT GCAGCCCAGC AACAACATCA GCATCCACAC 
CAGCAGCATC CCCAGCAGCA GCAGCAACAG CAGCAGGCGC AGAACCAGGG GCATCCACAT 
CACCTTATGG GCGGTGGCAA TGGACTGGGC AACGGCAATG GATTGGGCAT ACAACATCCC 
GGCCAGCAAC AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACATCCCGG CCAGTACAAC 
GCGAATCTGC TTAACCATGC GGCTGCCTTG GGTCACATGT CATCTTATGC CCAATCGGGT 
GGCAGCATGT ACGACCATCA TGGTGGAGCC ATGCACCCGG GAATGAACGG CGGCATGCCC 
AAGCAACAGC CATTGGGTCC ACCCGGAGCC GGAGGACCCC AGGACTATGT CTACATGGGT 
GGCCAGACCA CTGTGCCCAT GGGAGCCGCA ATGATGCCGC CACAGAATCA ATATATGAAC 
AGCTCTGCTG TTGCAGCTGC CAATCGGAAT GCAGCGATTA CCACATCCAC TGCCAAGAAA 
TTGTGGGAGA AATCCGATGG CAAGGGCGTA TCCTCGAGCA CTCCCGGTGG ACCGTTGCAT 
CCCCTGCAGA TCCCCGGCAT CGGGGATCCC TCCTCCGTGT GGAAGGATCA CACCTGGTCC 
ACACAGGGCG AGAATATATT GGTGCCGCCC CCCTCGCGAG CCTACGCCCA TGGAGGCGCC 
TCCGATACTT CAAACAGCGG CAATGCGGGC ATACTGAGTC CCCGCGATTC GACTTGCGCC 
AAAGTGGTTG AATATGTTTT CAGTGGCTCG CCCACCAACA AAGATAGCTC GCTTTCCGGA 
TTGGAACCGC ATTTGCGGAA TCTAAAGTTT GACGACAACG ATAAGTCACG CGACGATAAG 
GAGAAAGCAA ACTCTCCGTT TGACACAAAC GGTTTGAAGA AAGACGATCA GGTCACAAAC 
TCAAATGGTG TTGTCAACGG CATTGACGAT GACAAGGGCT TCAATCGCAC TCCTGGTTCA 
CGTCAACCAT CACCTGCAGA GGAGTCCCAG CCACGTCCCC CCAATCTACT CTTTCCTCCA 
CTGCCCTTCA ATCACATGCT CATGGATCAT GGCCAAGGCA TGGGAGGCGG CTTGGGCGGA 
GTTGTTGGAT CTGGCAACGG AGTCGGCGGT GGCAGCGGCG GAGGCGGGGC AGGCGGCGCT 
TATGCGGCCC ACCAGCAGAT GGCCGCCCAG ATGAGTCAAT TGCAACCGCC GATGATGAAC 
GGCGTTGGCG GCGGAATGCC AATGGCAGCA CAGTCACCAA TGTTGAATCA CCAGGCAGCT 
GGACCCAATC ACATGGAATC TCCCGGAAAT CTCTTGCAGC AGCAAAATTT TGATGTTCAG 
CAACTGTTTC GCTCGCAGAA TCCGGGCCTA GCAGCAGTTG CCACAAATGC AGCGGCCGCA 
GCAGCAGCCG CAGCAGCTGC CACATCGGCA GCGAGTGCTG CGGCAGCGGT GGGCGCACCA 
CCCGTTCCCA ACGGATCGCT GCAGCAGTCG CAGCAGCAAC AGCAGCAGCA GCAACAACAG 
CAGCAGCAAC AACAGATGCA CATGGCGGCC GCGTCGCAAC AATTTTTGGC CGCCCAGCAG 
CAGGCGCAAA ATGCGGCCTA TGCCGCCCAA CAGGCCACGT CCTACGTCAT CAATCCGGGC 
CAGGAGGCTG CCCCGTATAT GGGCATGATT GCCGCCGCCC AGATGCCGTA CTATGGCGTA 
GCACCATGGG GCATGTATCC GGGCAATCTG ATTCCGCAAC AGGGAACGCA GCCGCGCCGC 
CCCCTCACCC CCTCGCAGCA GGGTGCCGAG AATCAGCCGT ATCAGGTCAT CCCGGCATTC 
CTCGATCACA CGGGCTCCTT GCTGATGGGA GGACCTCGCA CCGGGACGCC GATGCGTCTG 
GTTAGCCCCG CCCCCGTTCT GGTGCCCCCG GGCGCTACCC GTGCCGGCCC CCCGCCCCCG 
CAGGGGCCAC AGCTGTATCA GCCGCAGCCG CAGACGGCCC AACAGAATCT CTACTCGCAG 
CAGAATGGAT CCAGTGTCGG AGGCCTCGCC TTGAACACGA GCTCGTTGAC GGGTCGCCGC 
GACTCCTTCG ACCGCAGCAC CTCCGCCTTC AGTCCCTCGA CCATGGACTA CACCAGCAGC 
GGTGTGGCAG CGGCCGCCAA TGCGGTGAAC AGCACAGTGG CCCAGGCAGC AGCAGCTGCC 
GCAGCAGCCG CCGCAGCGCG TGGCAAGTGG CCGGGAGCGA TGTCGGGAGC GGCCAGTGGA 
GCCTACGGAG CCCTGGGAGC GGGCAATGCT TCGGCCAGTC CCCTGGGCGC ACCAATCACG 
CCGCCGCCAT CGGCGCAATC CTGTCTCCTG GGCAGTCGGG CACCTGGAGC CGAGTCCCGC 
CAGCGGCAGC AGCAACAACA GCAGCTGGCC GCCGTTGGTC TGCCGGCGAC TGCAGCAGCT 
GCTCAGGCAG CGGTGGCCGC GGCTGCCAAC AATATGTTCG GATCCAACAG CTCGATCTTC 
TCGAATCCCC TGGCCATTCC GGGTACCGCA GCTGTGGCAG CTGCAGCGGC AGCAGCAGCG 
GCCGCCAACT CGCGTCAGGT GGCTGCCACG GCAGCGGCAG CAGCGGCGGT GGCAGCAGCA 
GCCGGCGGAG TGGGAGGTGC CCCACAGCCA GGAAGATCTC GCCTTCTCGA AGATTTCCGC 
AACCAGCGGT ATCCAAATCT TCAGCTACGC GATCTCGCTA ACCACATTGT GGAGTTCTCA 
CAGGATCAGC ACGGCTCGCG GTTTATCCAA CAGAAGTTGG AGCGGGCCAC CGCCGCCGAG 
AAGCAAATGG TGTTCAGCGA GATCCTGGCG GCAGCCTATA GCCTGATGAC CGATGTCTTT 
GGCAACTATG TCATCCAGAA GTTCTTTGAG TTCGGCACTC CCGAGCAGAA GAACACGCTG 
GGCATGCAGG TCAAGGGTCA TGTGCTGCAG CTGGCGCTGC AAATGTATGG CTGCCGAGTG 
ATTCAGAAGG CTCTGGAGAG CATCTCGCCG GAGCAGCAGC AGGAAATCGT CCACGAACTG 
GACGGACATG TGCTGAAATG CGTCAAGGAT CAGAATGGCA ATCATGTGGT GCAAAAGTGC 
ATTGAGTGCG TGGACCCCGT GGCGCTGCAG TTCATCATCA ATGCTTTCAA GGGTCAGGTT 
TACTCGCTAA GCACCCATCC GTATGGATGC CGGGTGATCC AGAGAATCCT TGAGCATTGC 
ACTGCCGAAC AGACCACGCC CATTTTGGAC GAACTGCATG AGCACACCGA ACAGTTGATT 
CAGGACCAAT ATGGCAACTA TGTTATTCAG CATGTGCTTG AACACGGCAA GCAGGAGGAT 
AAGTCGATTC TTATCAACAG CGTGCGCGGC AAAGTTCTGG TGCTATCACA GCACAAGTTC 
GCCTCAAACG TTGTGGAGAA ATGTGTTACC CATGCCACTC GCGGAGAACG CACTGGTCTC 
ATAGACGAGG TCTGCACCTT CAACGACAAC GCGTTGCACG TGATGATGAA GGATCAGTAT 
GCCAACTATG TGGTCCAAAA AATGATCGAT GTATCGGAGC CGACGCAGCT CAAGAAGCTG 
ATGACCAAGA TCCGGCCCCA CATGGCCGCC TTGCGCAAGT ACACCTACGG CAAGCACATC 
AATGCCAAGT TGGAGAAGTA CTACATGAAG ATAACCAATC CCATTACGGT GGGCACAGGA 
GCTGGAGGAG TGCCGGCAGC CTCGTCGGCG GCGGCAGTCA GCAGTGGTGC CACCTCGGCA 
TCGGTAACCG CCTGCACCAG TGGCAGCAGC ACCACCACGA CCAGCACTAC CAACAGCCTG 
GCCTCACCCA CCATTTGTTC GGTGCAGGAG AACGGCAGCG CCATGGTTGT GGAGCCCTCC 
TCCCCGGACG CCTCCGAGTC CTCGTCCTCG GTGGTGTCAG GCGCTGTCAA CAGCAGCTTG 
GGTCCCATTG GACCCCCGAC CAACGGCAAC GTTGTGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524285.2
CDS	1020..5621
Translation	MKFLGGNDDRNGRGGVGVGTDAIVGSRGGVSQDAADAAGAAAAAAVGYVFQQRPSPGGVGVGVGGVGGGVPGVGAVGSTLHEAAAAEYAAHFAQKQQQTRWACGDDGHGIDNPDKWKYNPPMNPANAAPGGPPGNGSNGGPGAIGTIGMGSGLGGGGGGGAGGGNNGGSGTNGGLHHQSMAAAAANMAAMQQAAALAKHNHMISQAAAAVAAQQQHQHPHQQHPQQQQQQQQAQNQGHPHHLMGGGNGLGNGNGLGIQHPGQQQQQQQQQQQQQHPGQYNANLLNHAAALGHMSSYAQSGGSMYDHHGGAMHPGMNGGMPKQQPLGPPGAGGPQDYVYMGGQTTVPMGAAMMPPQNQYMNSSAVAAANRNAAITTSTAKKLWEKSDGKGVSSSTPGGPLHPLQIPGIGDPSSVWKDHTWSTQGENILVPPPSRAYAHGGASDTSNSGNAGILSPRDSTCAKVVEYVFSGSPTNKDSSLSGLEPHLRNLKFDDNDKSRDDKEKANSPFDTNGLKKDDQVTNSNGVVNGIDDDKGFNRTPGSRQPSPAEESQPRPPNLLFPPLPFNHMLMDHGQGMGGGLGGVVGSGNGVGGGSGGGGAGGAYAAHQQMAAQMSQLQPPMMNGVGGGMPMAAQSPMLNHQAAGPNHMESPGNLLQQQNFDVQQLFRSQNPGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQQQQQQMHMAAASQQFLAAQQQAQNAAYAAQQATSYVINPGQEAAPYMGMIAAAQMPYYGVAPWGMYPGNLIPQQGTQPRRPLTPSQQGAENQPYQVIPAFLDHTGSLLMGGPRTGTPMRLVSPAPVLVPPGATRAGPPPPQGPQLYQPQPQTAQQNLYSQQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPSTMDYTSSGVAAAANAVNSTVAQAAAAAAAAAAARGKWPGAMSGAASGAYGALGAGNASASPLGAPITPPPSAQSCLLGSRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCTFNDNALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLEKYYMKITNPITVGTGAGGVPAASSAAAVSSGATSASVTACTSGSSTTTTSTTNSLASPTICSVQENGSAMVVEPSSPDASESSSSVVSGAVNSSLGPIGPPTNGNVVL

Target ORF information:

RefSeq Version	NM_079561.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pumilio (pum), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079561.4

ORF Insert Sequence:

ATGAAGTTTT TGGGTGGTAA CGATGATCGC AATGGCCGCG GAGGCGTCGG CGTTGGCACG 
GATGCCATTG TAGGATCTCG AGGTGGCGTC TCTCAGGATG CCGCCGATGC AGCTGGTGCC 
GCCGCAGCCG CCGCCGTCGG CTATGTCTTC CAGCAGCGTC CATCGCCTGG TGGGGTTGGC 
GTCGGCGTGG GCGGAGTGGG TGGCGGTGTG CCAGGGGTCG GAGCCGTAGG CTCGACCTTG 
CACGAGGCCG CCGCCGCCGA GTACGCCGCC CACTTTGCCC AGAAGCAACA GCAGACCCGA 
TGGGCGTGCG GCGACGACGG CCATGGGATC GATAACCCGG ACAAATGGAA GTACAATCCG 
CCGATGAATC CGGCCAATGC CGCTCCTGGC GGTCCACCGG GAAATGGCAG TAATGGTGGG 
CCCGGCGCCA TTGGAACCAT TGGCATGGGC AGCGGATTGG GTGGTGGTGG CGGCGGCGGA 
GCTGGCGGCG GAAATAATGG CGGCTCTGGT ACGAATGGCG GTCTGCATCA TCAATCGATG 
GCCGCTGCAG CTGCGAATAT GGCAGCCATG CAACAGGCGG CGGCGTTGGC CAAGCACAAT 
CACATGATAT CACAGGCAGC AGCCGCAGTT GCAGCCCAGC AACAACATCA GCATCCACAC 
CAGCAGCATC CCCAGCAGCA GCAGCAACAG CAGCAGGCGC AGAACCAGGG GCATCCACAT 
CACCTTATGG GCGGTGGCAA TGGACTGGGC AACGGCAATG GATTGGGCAT ACAACATCCC 
GGCCAGCAAC AGCAGCAGCA GCAGCAACAA CAGCAGCAGC AACATCCCGG CCAGTACAAC 
GCGAATCTGC TTAACCATGC GGCTGCCTTG GGTCACATGT CATCTTATGC CCAATCGGGT 
GGCAGCATGT ACGACCATCA TGGTGGAGCC ATGCACCCGG GAATGAACGG CGGCATGCCC 
AAGCAACAGC CATTGGGTCC ACCCGGAGCC GGAGGACCCC AGGACTATGT CTACATGGGT 
GGCCAGACCA CTGTGCCCAT GGGAGCCGCA ATGATGCCGC CACAGAATCA ATATATGAAC 
AGCTCTGCTG TTGCAGCTGC CAATCGGAAT GCAGCGATTA CCACATCCAC TGCCAAGAAA 
TTGTGGGAGA AATCCGATGG CAAGGGCGTA TCCTCGAGCA CTCCCGGTGG ACCGTTGCAT 
CCCCTGCAGA TCCCCGGCAT CGGGGATCCC TCCTCCGTGT GGAAGGATCA CACCTGGTCC 
ACACAGGGCG AGAATATATT GGTGCCGCCC CCCTCGCGAG CCTACGCCCA TGGAGGCGCC 
TCCGATACTT CAAACAGCGG CAATGCGGGC ATACTGAGTC CCCGCGATTC GACTTGCGCC 
AAAGTGGTTG AATATGTTTT CAGTGGCTCG CCCACCAACA AAGATAGCTC GCTTTCCGGA 
TTGGAACCGC ATTTGCGGAA TCTAAAGTTT GACGACAACG ATAAGTCACG CGACGATAAG 
GAGAAAGCAA ACTCTCCGTT TGACACAAAC GGTTTGAAGA AAGACGATCA GGTCACAAAC 
TCAAATGGTG TTGTCAACGG CATTGACGAT GACAAGGGCT TCAATCGCAC TCCTGGTTCA 
CGTCAACCAT CACCTGCAGA GGAGTCCCAG CCACGTCCCC CCAATCTACT CTTTCCTCCA 
CTGCCCTTCA ATCACATGCT CATGGATCAT GGCCAAGGCA TGGGAGGCGG CTTGGGCGGA 
GTTGTTGGAT CTGGCAACGG AGTCGGCGGT GGCAGCGGCG GAGGCGGGGC AGGCGGCGCT 
TATGCGGCCC ACCAGCAGAT GGCCGCCCAG ATGAGTCAAT TGCAACCGCC GATGATGAAC 
GGCGTTGGCG GCGGAATGCC AATGGCAGCA CAGTCACCAA TGTTGAATCA CCAGGCAGCT 
GGACCCAATC ACATGGAATC TCCCGGAAAT CTCTTGCAGC AGCAAAATTT TGATGTTCAG 
CAACTGTTTC GCTCGCAGAA TCCGGGCCTA GCAGCAGTTG CCACAAATGC AGCGGCCGCA 
GCAGCAGCCG CAGCAGCTGC CACATCGGCA GCGAGTGCTG CGGCAGCGGT GGGCGCACCA 
CCCGTTCCCA ACGGATCGCT GCAGCAGTCG CAGCAGCAAC AGCAGCAGCA GCAACAACAG 
CAGCAGCAAC AACAGATGCA CATGGCGGCC GCGTCGCAAC AATTTTTGGC CGCCCAGCAG 
CAGGCGCAAA ATGCGGCCTA TGCCGCCCAA CAGGCCACGT CCTACGTCAT CAATCCGGGC 
CAGGAGGCTG CCCCGTATAT GGGCATGATT GCCGCCGCCC AGATGCCGTA CTATGGCGTA 
GCACCATGGG GCATGTATCC GGGCAATCTG ATTCCGCAAC AGGGAACGCA GCCGCGCCGC 
CCCCTCACCC CCTCGCAGCA GGGTGCCGAG AATCAGCCGT ATCAGGTCAT CCCGGCATTC 
CTCGATCACA CGGGCTCCTT GCTGATGGGA GGACCTCGCA CCGGGACGCC GATGCGTCTG 
GTTAGCCCCG CCCCCGTTCT GGTGCCCCCG GGCGCTACCC GTGCCGGCCC CCCGCCCCCG 
CAGGGGCCAC AGCTGTATCA GCCGCAGCCG CAGACGGCCC AACAGAATCT CTACTCGCAG 
CAGAATGGAT CCAGTGTCGG AGGCCTCGCC TTGAACACGA GCTCGTTGAC GGGTCGCCGC 
GACTCCTTCG ACCGCAGCAC CTCCGCCTTC AGTCCCTCGA CCATGGACTA CACCAGCAGC 
GGTGTGGCAG CGGCCGCCAA TGCGGTGAAC AGCACAGTGG CCCAGGCAGC AGCAGCTGCC 
GCAGCAGCCG CCGCAGCGCG TGGCAAGTGG CCGGGAGCGA TGTCGGGAGC GGCCAGTGGA 
GCCTACGGAG CCCTGGGAGC GGGCAATGCT TCGGCCAGTC CCCTGGGCGC ACCAATCACG 
CCGCCGCCAT CGGCGCAATC CTGTCTCCTG GGCAGTCGGG CACCTGGAGC CGAGTCCCGC 
CAGCGGCAGC AGCAACAACA GCAGCTGGCC GCCGTTGGTC TGCCGGCGAC TGCAGCAGCT 
GCTCAGGCAG CGGTGGCCGC GGCTGCCAAC AATATGTTCG GATCCAACAG CTCGATCTTC 
TCGAATCCCC TGGCCATTCC GGGTACCGCA GCTGTGGCAG CTGCAGCGGC AGCAGCAGCG 
GCCGCCAACT CGCGTCAGGT GGCTGCCACG GCAGCGGCAG CAGCGGCGGT GGCAGCAGCA 
GCCGGCGGAG TGGGAGGTGC CCCACAGCCA GGAAGATCTC GCCTTCTCGA AGATTTCCGC 
AACCAGCGGT ATCCAAATCT TCAGCTACGC GATCTCGCTA ACCACATTGT GGAGTTCTCA 
CAGGATCAGC ACGGCTCGCG GTTTATCCAA CAGAAGTTGG AGCGGGCCAC CGCCGCCGAG 
AAGCAAATGG TGTTCAGCGA GATCCTGGCG GCAGCCTATA GCCTGATGAC CGATGTCTTT 
GGCAACTATG TCATCCAGAA GTTCTTTGAG TTCGGCACTC CCGAGCAGAA GAACACGCTG 
GGCATGCAGG TCAAGGGTCA TGTGCTGCAG CTGGCGCTGC AAATGTATGG CTGCCGAGTG 
ATTCAGAAGG CTCTGGAGAG CATCTCGCCG GAGCAGCAGC AGGAAATCGT CCACGAACTG 
GACGGACATG TGCTGAAATG CGTCAAGGAT CAGAATGGCA ATCATGTGGT GCAAAAGTGC 
ATTGAGTGCG TGGACCCCGT GGCGCTGCAG TTCATCATCA ATGCTTTCAA GGGTCAGGTT 
TACTCGCTAA GCACCCATCC GTATGGATGC CGGGTGATCC AGAGAATCCT TGAGCATTGC 
ACTGCCGAAC AGACCACGCC CATTTTGGAC GAACTGCATG AGCACACCGA ACAGTTGATT 
CAGGACCAAT ATGGCAACTA TGTTATTCAG CATGTGCTTG AACACGGCAA GCAGGAGGAT 
AAGTCGATTC TTATCAACAG CGTGCGCGGC AAAGTTCTGG TGCTATCACA GCACAAGTTC 
GCCTCAAACG TTGTGGAGAA ATGTGTTACC CATGCCACTC GCGGAGAACG CACTGGTCTC 
ATAGACGAGG TCTGCACCTT CAACGACAAC GCGTTGCACG TGATGATGAA GGATCAGTAT 
GCCAACTATG TGGTCCAAAA AATGATCGAT GTATCGGAGC CGACGCAGCT CAAGAAGCTG 
ATGACCAAGA TCCGGCCCCA CATGGCCGCC TTGCGCAAGT ACACCTACGG CAAGCACATC 
AATGCCAAGT TGGAGAAGTA CTACATGAAG ATAACCAATC CCATTACGGT GGGCACAGGA 
GCTGGAGGAG TGCCGGCAGC CTCGTCGGCG GCGGCAGTCA GCAGTGGTGC CACCTCGGCA 
TCGGTAACCG CCTGCACCAG TGGCAGCAGC ACCACCACGA CCAGCACTAC CAACAGCCTG 
GCCTCACCCA CCATTTGTTC GGTGCAGGAG AACGGCAGCG CCATGGTTGT GGAGCCCTCC 
TCCCCGGACG CCTCCGAGTC CTCGTCCTCG GTGGTGTCAG GCGCTGTCAA CAGCAGCTTG 
GGTCCCATTG GACCCCCGAC CAACGGCAAC GTTGTGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum ( NM_001275475.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262404.1 Drosophila melanogaster pumilio (pum), transcript variant H, mRNA.

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CloneID	OFa04318
Clone ID Related Accession (Same CDS sequence)	NM_001275475.1
Accession Version	NM_001275475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pumilio, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGTTT TAGAAACTGC CAGCGCCCTT TTGGGCGGCC CGTATGCCCA GGGCGCTCCC 
GCCCTGAAAA TGGTGCAGAA ACGCTACATT GGATTACATC ACTGGCTGGG ACCAATCCGC 
TCCAAGGAGC TAAAGGAGCA TATTCAACTG TTTCGCTCGC AGAATCCGGG CCTAGCAGCA 
GTTGCCACAA ATGCAGCGGC CGCAGCAGCA GCCGCAGCAG CTGCCACATC GGCAGCGAGT 
GCTGCGGCAG CGGTGGGCGC ACCACCCGTT CCCAACGGAT CGCTGCAGCA GTCGCAGCAG 
CAACAGCAGC AGCAGCAACA ACAGCAGCAG CAACAACAGA TGCACATGGC GGCCGCGTCG 
CAACAATTTT TGGCCGCCCA GCAGCAGGCG CAAAATGCGG CCTATGCCGC CCAACAGGCC 
ACGTCCTACG TCATCAATCC GGGCCAGGAG GCTGCCCCGT ATATGGGCAT GATTGCCGCC 
GCCCAGATGC CGTACTATGG CGTAGCACCA TGGGGCATGT ATCCGGGCAA TCTGATTCCG 
CAACAGGGAA CGCAGCCGCG CCGCCCCCTC ACCCCCTCGC AGCAGGGTGC CGAGAATCAG 
CCGTATCAGG TCATCCCGGC ATTCCTCGAT CACACGGGCT CCTTGCTGAT GGGAGGACCT 
CGCACCGGGA CGCCGATGCG TCTGGTTAGC CCCGCCCCCG TTCTGGTGCC CCCGGGCGCT 
ACCCGTGCCG GCCCCCCGCC CCCGCAGGGG CCACAGCTGT ATCAGCCGCA GCCGCAGACG 
GCCCAACAGA ATCTCTACTC GCAGCAGAAT GGATCCAGTG TCGGAGGCCT CGCCTTGAAC 
ACGAGCTCGT TGACGGGTCG CCGCGACTCC TTCGACCGCA GCACCTCCGC CTTCAGTCCC 
TCGACCATGG ACTACACCAG CAGCGGTGTG GCAGCGGCCG CCAATGCGGT GAACAGCACA 
GTGGCCCAGG CAGCAGCAGC TGCCGCAGCA GCCGCCGCAG CGCGTGGCAA GTGGCCGGGA 
GCGATGTCGG GAGCGGCCAG TGGAGCCTAC GGAGCCCTGG GAGCGGGCAA TGCTTCGGCC 
AGTCCCCTGG GCGCACCAAT CACGCCGCCG CCATCGGCGC AATCCTGTCT CCTGGGCAGT 
CGGGCACCTG GAGCCGAGTC CCGCCAGCGG CAGCAGCAAC AACAGCAGCT GGCCGCCGTT 
GGTCTGCCGG CGACTGCAGC AGCTGCTCAG GCAGCGGTGG CCGCGGCTGC CAACAATATG 
TTCGGATCCA ACAGCTCGAT CTTCTCGAAT CCCCTGGCCA TTCCGGGTAC CGCAGCTGTG 
GCAGCTGCAG CGGCAGCAGC AGCGGCCGCC AACTCGCGTC AGGTGGCTGC CACGGCAGCG 
GCAGCAGCGG CGGTGGCAGC AGCAGCCGGC GGAGTGGGAG GTGCCCCACA GCCAGGAAGA 
TCTCGCCTTC TCGAAGATTT CCGCAACCAG CGGTATCCAA ATCTTCAGCT ACGCGATCTC 
GCTAACCACA TTGTGGAGTT CTCACAGGAT CAGCACGGCT CGCGGTTTAT CCAACAGAAG 
TTGGAGCGGG CCACCGCCGC CGAGAAGCAA ATGGTGTTCA GCGAGATCCT GGCGGCAGCC 
TATAGCCTGA TGACCGATGT CTTTGGCAAC TATGTCATCC AGAAGTTCTT TGAGTTCGGC 
ACTCCCGAGC AGAAGAACAC GCTGGGCATG CAGGTCAAGG GTCATGTGCT GCAGCTGGCG 
CTGCAAATGT ATGGCTGCCG AGTGATTCAG AAGGCTCTGG AGAGCATCTC GCCGGAGCAG 
CAGCAGGAAA TCGTCCACGA ACTGGACGGA CATGTGCTGA AATGCGTCAA GGATCAGAAT 
GGCAATCATG TGGTGCAAAA GTGCATTGAG TGCGTGGACC CCGTGGCGCT GCAGTTCATC 
ATCAATGCTT TCAAGGGTCA GGTTTACTCG CTAAGCACCC ATCCGTATGG ATGCCGGGTG 
ATCCAGAGAA TCCTTGAGCA TTGCACTGCC GAACAGACCA CGCCCATTTT GGACGAACTG 
CATGAGCACA CCGAACAGTT GATTCAGGAC CAATATGGCA ACTATGTTAT TCAGCATGTG 
CTTGAACACG GCAAGCAGGA GGATAAGTCG ATTCTTATCA ACAGCGTGCG CGGCAAAGTT 
CTGGTGCTAT CACAGCACAA GTTCGCCTCA AACGTTGTGG AGAAATGTGT TACCCATGCC 
ACTCGCGGAG AACGCACTGG TCTCATAGAC GAGGTCTGCA CCTTCAACGA CAACGCGTTG 
CACGTGATGA TGAAGGATCA GTATGCCAAC TATGTGGTCC AAAAAATGAT CGATGTATCG 
GAGCCGACGC AGCTCAAGAA GCTGATGACC AAGATCCGGC CCCACATGGC CGCCTTGCGC 
AAGTACACCT ACGGCAAGCA CATCAATGCC AAGTTGGAGA AGTACTACAT GAAGATAACC 
AATCCCATTA CGGTGGGCAC AGGAGCTGGA GGAGTGCCGG CAGCCTCGTC GGCGGCGGCA 
GTCAGCAGTG GTGCCACCTC GGCATCGGTA ACCGCCTGCA CCAGTGGCAG CAGCACCACC 
ACGACCAGCA CTACCAACAG CCTGGCCTCA CCCACCATTT GTTCGGTGCA GGAGAACGGC 
AGCGCCATGG TTGTGGAGCC CTCCTCCCCG GACGCCTCCG AGTCCTCGTC CTCGGTGGTG 
TCAGGCGCTG TCAACAGCAG CTTGGGTCCC ATTGGACCCC CGACCAACGG CAACGTTGTG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262404.1
CDS	690..3455
Translation	MVVLETASALLGGPYAQGAPALKMVQKRYIGLHHWLGPIRSKELKEHIQLFRSQNPGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQQQQQQMHMAAASQQFLAAQQQAQNAAYAAQQATSYVINPGQEAAPYMGMIAAAQMPYYGVAPWGMYPGNLIPQQGTQPRRPLTPSQQGAENQPYQVIPAFLDHTGSLLMGGPRTGTPMRLVSPAPVLVPPGATRAGPPPPQGPQLYQPQPQTAQQNLYSQQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPSTMDYTSSGVAAAANAVNSTVAQAAAAAAAAAAARGKWPGAMSGAASGAYGALGAGNASASPLGAPITPPPSAQSCLLGSRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCTFNDNALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLEKYYMKITNPITVGTGAGGVPAASSAAAVSSGATSASVTACTSGSSTTTTSTTNSLASPTICSVQENGSAMVVEPSSPDASESSSSVVSGAVNSSLGPIGPPTNGNVVL

Target ORF information:

RefSeq Version	NM_001275475.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pumilio (pum), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275475.1

ORF Insert Sequence:

ATGGTGGTTT TAGAAACTGC CAGCGCCCTT TTGGGCGGCC CGTATGCCCA GGGCGCTCCC 
GCCCTGAAAA TGGTGCAGAA ACGCTACATT GGATTACATC ACTGGCTGGG ACCAATCCGC 
TCCAAGGAGC TAAAGGAGCA TATTCAACTG TTTCGCTCGC AGAATCCGGG CCTAGCAGCA 
GTTGCCACAA ATGCAGCGGC CGCAGCAGCA GCCGCAGCAG CTGCCACATC GGCAGCGAGT 
GCTGCGGCAG CGGTGGGCGC ACCACCCGTT CCCAACGGAT CGCTGCAGCA GTCGCAGCAG 
CAACAGCAGC AGCAGCAACA ACAGCAGCAG CAACAACAGA TGCACATGGC GGCCGCGTCG 
CAACAATTTT TGGCCGCCCA GCAGCAGGCG CAAAATGCGG CCTATGCCGC CCAACAGGCC 
ACGTCCTACG TCATCAATCC GGGCCAGGAG GCTGCCCCGT ATATGGGCAT GATTGCCGCC 
GCCCAGATGC CGTACTATGG CGTAGCACCA TGGGGCATGT ATCCGGGCAA TCTGATTCCG 
CAACAGGGAA CGCAGCCGCG CCGCCCCCTC ACCCCCTCGC AGCAGGGTGC CGAGAATCAG 
CCGTATCAGG TCATCCCGGC ATTCCTCGAT CACACGGGCT CCTTGCTGAT GGGAGGACCT 
CGCACCGGGA CGCCGATGCG TCTGGTTAGC CCCGCCCCCG TTCTGGTGCC CCCGGGCGCT 
ACCCGTGCCG GCCCCCCGCC CCCGCAGGGG CCACAGCTGT ATCAGCCGCA GCCGCAGACG 
GCCCAACAGA ATCTCTACTC GCAGCAGAAT GGATCCAGTG TCGGAGGCCT CGCCTTGAAC 
ACGAGCTCGT TGACGGGTCG CCGCGACTCC TTCGACCGCA GCACCTCCGC CTTCAGTCCC 
TCGACCATGG ACTACACCAG CAGCGGTGTG GCAGCGGCCG CCAATGCGGT GAACAGCACA 
GTGGCCCAGG CAGCAGCAGC TGCCGCAGCA GCCGCCGCAG CGCGTGGCAA GTGGCCGGGA 
GCGATGTCGG GAGCGGCCAG TGGAGCCTAC GGAGCCCTGG GAGCGGGCAA TGCTTCGGCC 
AGTCCCCTGG GCGCACCAAT CACGCCGCCG CCATCGGCGC AATCCTGTCT CCTGGGCAGT 
CGGGCACCTG GAGCCGAGTC CCGCCAGCGG CAGCAGCAAC AACAGCAGCT GGCCGCCGTT 
GGTCTGCCGG CGACTGCAGC AGCTGCTCAG GCAGCGGTGG CCGCGGCTGC CAACAATATG 
TTCGGATCCA ACAGCTCGAT CTTCTCGAAT CCCCTGGCCA TTCCGGGTAC CGCAGCTGTG 
GCAGCTGCAG CGGCAGCAGC AGCGGCCGCC AACTCGCGTC AGGTGGCTGC CACGGCAGCG 
GCAGCAGCGG CGGTGGCAGC AGCAGCCGGC GGAGTGGGAG GTGCCCCACA GCCAGGAAGA 
TCTCGCCTTC TCGAAGATTT CCGCAACCAG CGGTATCCAA ATCTTCAGCT ACGCGATCTC 
GCTAACCACA TTGTGGAGTT CTCACAGGAT CAGCACGGCT CGCGGTTTAT CCAACAGAAG 
TTGGAGCGGG CCACCGCCGC CGAGAAGCAA ATGGTGTTCA GCGAGATCCT GGCGGCAGCC 
TATAGCCTGA TGACCGATGT CTTTGGCAAC TATGTCATCC AGAAGTTCTT TGAGTTCGGC 
ACTCCCGAGC AGAAGAACAC GCTGGGCATG CAGGTCAAGG GTCATGTGCT GCAGCTGGCG 
CTGCAAATGT ATGGCTGCCG AGTGATTCAG AAGGCTCTGG AGAGCATCTC GCCGGAGCAG 
CAGCAGGAAA TCGTCCACGA ACTGGACGGA CATGTGCTGA AATGCGTCAA GGATCAGAAT 
GGCAATCATG TGGTGCAAAA GTGCATTGAG TGCGTGGACC CCGTGGCGCT GCAGTTCATC 
ATCAATGCTT TCAAGGGTCA GGTTTACTCG CTAAGCACCC ATCCGTATGG ATGCCGGGTG 
ATCCAGAGAA TCCTTGAGCA TTGCACTGCC GAACAGACCA CGCCCATTTT GGACGAACTG 
CATGAGCACA CCGAACAGTT GATTCAGGAC CAATATGGCA ACTATGTTAT TCAGCATGTG 
CTTGAACACG GCAAGCAGGA GGATAAGTCG ATTCTTATCA ACAGCGTGCG CGGCAAAGTT 
CTGGTGCTAT CACAGCACAA GTTCGCCTCA AACGTTGTGG AGAAATGTGT TACCCATGCC 
ACTCGCGGAG AACGCACTGG TCTCATAGAC GAGGTCTGCA CCTTCAACGA CAACGCGTTG 
CACGTGATGA TGAAGGATCA GTATGCCAAC TATGTGGTCC AAAAAATGAT CGATGTATCG 
GAGCCGACGC AGCTCAAGAA GCTGATGACC AAGATCCGGC CCCACATGGC CGCCTTGCGC 
AAGTACACCT ACGGCAAGCA CATCAATGCC AAGTTGGAGA AGTACTACAT GAAGATAACC 
AATCCCATTA CGGTGGGCAC AGGAGCTGGA GGAGTGCCGG CAGCCTCGTC GGCGGCGGCA 
GTCAGCAGTG GTGCCACCTC GGCATCGGTA ACCGCCTGCA CCAGTGGCAG CAGCACCACC 
ACGACCAGCA CTACCAACAG CCTGGCCTCA CCCACCATTT GTTCGGTGCA GGAGAACGGC 
AGCGCCATGG TTGTGGAGCC CTCCTCCCCG GACGCCTCCG AGTCCTCGTC CTCGGTGGTG 
TCAGGCGCTG TCAACAGCAG CTTGGGTCCC ATTGGACCCC CGACCAACGG CAACGTTGTG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum ( NM_001260073.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247002.1 Drosophila melanogaster pumilio (pum), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa04319
Clone ID Related Accession (Same CDS sequence)	NM_001260073.2
Accession Version	NM_001260073.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pumilio, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260073.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGTTT TAGAAACTGC CAGCGCCCTT TTGGGCGGCC CGTATGCCCA GGGCGCTCCC 
GCCCTGAAAA TGGTGCAGAA ACGCTACATT GGATTACATC ACTGGCTGGG ACCAATCCGC 
TCCAAGGAGC TAAAGGAGCA TATTGTAGGT GACAATCAAC TGTTTCGCTC GCAGAATCCG 
GGCCTAGCAG CAGTTGCCAC AAATGCAGCG GCCGCAGCAG CAGCCGCAGC AGCTGCCACA 
TCGGCAGCGA GTGCTGCGGC AGCGGTGGGC GCACCACCCG TTCCCAACGG ATCGCTGCAG 
CAGTCGCAGC AGCAACAGCA GCAGCAGCAA CAACAGCAGC AGCAACAACA GATGCACATG 
GCGGCCGCGT CGCAACAATT TTTGGCCGCC CAGCAGCAGG CGCAAAATGC GGCCTATGCC 
GCCCAACAGG CCACGTCCTA CGTCATCAAT CCGGGCCAGG AGGCTGCCCC GTATATGGGC 
ATGATTGCCG CCGCCCAGAT GCCGTACTAT GGCGTAGCAC CATGGGGCAT GTATCCGGGC 
AATCTGATTC CGCAACAGGG AACGCAGCCG CGCCGCCCCC TCACCCCCTC GCAGCAGGGT 
GCCGAGAATC AGCCGTATCA GGTCATCCCG GCATTCCTCG ATCACACGGG CTCCTTGCTG 
ATGGGAGGAC CTCGCACCGG GACGCCGATG CGTCTGGTTA GCCCCGCCCC CGTTCTGGTG 
CCCCCGGGCG CTACCCGTGC CGGCCCCCCG CCCCCGCAGG GGCCACAGCT GTATCAGCCG 
CAGCCGCAGA CGGCCCAACA GAATCTCTAC TCGCAGCAGA ATGGATCCAG TGTCGGAGGC 
CTCGCCTTGA ACACGAGCTC GTTGACGGGT CGCCGCGACT CCTTCGACCG CAGCACCTCC 
GCCTTCAGTC CCTCGACCAT GGACTACACC AGCAGCGGTG TGGCAGCGGC CGCCAATGCG 
GTGAACAGCA CAGTGGCCCA GGCAGCAGCA GCTGCCGCAG CAGCCGCCGC AGCGCGTGGC 
AAGTGGCCGG GAGCGATGTC GGGAGCGGCC AGTGGAGCCT ACGGAGCCCT GGGAGCGGGC 
AATGCTTCGG CCAGTCCCCT GGGCGCACCA ATCACGCCGC CGCCATCGGC GCAATCCTGT 
CTCCTGGGCA GTCGGGCACC TGGAGCCGAG TCCCGCCAGC GGCAGCAGCA ACAACAGCAG 
CTGGCCGCCG TTGGTCTGCC GGCGACTGCA GCAGCTGCTC AGGCAGCGGT GGCCGCGGCT 
GCCAACAATA TGTTCGGATC CAACAGCTCG ATCTTCTCGA ATCCCCTGGC CATTCCGGGT 
ACCGCAGCTG TGGCAGCTGC AGCGGCAGCA GCAGCGGCCG CCAACTCGCG TCAGGTGGCT 
GCCACGGCAG CGGCAGCAGC GGCGGTGGCA GCAGCAGCCG GCGGAGTGGG AGGTGCCCCA 
CAGCCAGGAA GATCTCGCCT TCTCGAAGAT TTCCGCAACC AGCGGTATCC AAATCTTCAG 
CTACGCGATC TCGCTAACCA CATTGTGGAG TTCTCACAGG ATCAGCACGG CTCGCGGTTT 
ATCCAACAGA AGTTGGAGCG GGCCACCGCC GCCGAGAAGC AAATGGTGTT CAGCGAGATC 
CTGGCGGCAG CCTATAGCCT GATGACCGAT GTCTTTGGCA ACTATGTCAT CCAGAAGTTC 
TTTGAGTTCG GCACTCCCGA GCAGAAGAAC ACGCTGGGCA TGCAGGTCAA GGGTCATGTG 
CTGCAGCTGG CGCTGCAAAT GTATGGCTGC CGAGTGATTC AGAAGGCTCT GGAGAGCATC 
TCGCCGGAGC AGCAGCAGGA AATCGTCCAC GAACTGGACG GACATGTGCT GAAATGCGTC 
AAGGATCAGA ATGGCAATCA TGTGGTGCAA AAGTGCATTG AGTGCGTGGA CCCCGTGGCG 
CTGCAGTTCA TCATCAATGC TTTCAAGGGT CAGGTTTACT CGCTAAGCAC CCATCCGTAT 
GGATGCCGGG TGATCCAGAG AATCCTTGAG CATTGCACTG CCGAACAGAC CACGCCCATT 
TTGGACGAAC TGCATGAGCA CACCGAACAG TTGATTCAGG ACCAATATGG CAACTATGTT 
ATTCAGCATG TGCTTGAACA CGGCAAGCAG GAGGATAAGT CGATTCTTAT CAACAGCGTG 
CGCGGCAAAG TTCTGGTGCT ATCACAGCAC AAGTTCGCCT CAAACGTTGT GGAGAAATGT 
GTTACCCATG CCACTCGCGG AGAACGCACT GGTCTCATAG ACGAGGTCTG CACCTTCAAC 
GACAACGCGT TGCACGTGAT GATGAAGGAT CAGTATGCCA ACTATGTGGT CCAAAAAATG 
ATCGATGTAT CGGAGCCGAC GCAGCTCAAG AAGCTGATGA CCAAGATCCG GCCCCACATG 
GCCGCCTTGC GCAAGTACAC CTACGGCAAG CACATCAATG CCAAGTTGGA GAAGTACTAC 
ATGAAGATAA CCAATCCCAT TACGGTGGGC ACAGGAGCTG GAGGAGTGCC GGCAGCCTCG 
TCGGCGGCGG CAGTCAGCAG TGGTGCCACC TCGGCATCGG TAACCGCCTG CACCAGTGGC 
AGCAGCACCA CCACGACCAG CACTACCAAC AGCCTGGCCT CACCCACCAT TTGTTCGGTG 
CAGGAGAACG GCAGCGCCAT GGTTGTGGAG CCCTCCTCCC CGGACGCCTC CGAGTCCTCG 
TCCTCGGTGG TGTCAGGCGC TGTCAACAGC AGCTTGGGTC CCATTGGACC CCCGACCAAC 
GGCAACGTTG TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247002.1
CDS	690..3467
Translation	MVVLETASALLGGPYAQGAPALKMVQKRYIGLHHWLGPIRSKELKEHIVGDNQLFRSQNPGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQQQQQQMHMAAASQQFLAAQQQAQNAAYAAQQATSYVINPGQEAAPYMGMIAAAQMPYYGVAPWGMYPGNLIPQQGTQPRRPLTPSQQGAENQPYQVIPAFLDHTGSLLMGGPRTGTPMRLVSPAPVLVPPGATRAGPPPPQGPQLYQPQPQTAQQNLYSQQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPSTMDYTSSGVAAAANAVNSTVAQAAAAAAAAAAARGKWPGAMSGAASGAYGALGAGNASASPLGAPITPPPSAQSCLLGSRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCTFNDNALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLEKYYMKITNPITVGTGAGGVPAASSAAAVSSGATSASVTACTSGSSTTTTSTTNSLASPTICSVQENGSAMVVEPSSPDASESSSSVVSGAVNSSLGPIGPPTNGNVVL

Target ORF information:

RefSeq Version	NM_001260073.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pumilio (pum), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260073.2

ORF Insert Sequence:

ATGGTGGTTT TAGAAACTGC CAGCGCCCTT TTGGGCGGCC CGTATGCCCA GGGCGCTCCC 
GCCCTGAAAA TGGTGCAGAA ACGCTACATT GGATTACATC ACTGGCTGGG ACCAATCCGC 
TCCAAGGAGC TAAAGGAGCA TATTGTAGGT GACAATCAAC TGTTTCGCTC GCAGAATCCG 
GGCCTAGCAG CAGTTGCCAC AAATGCAGCG GCCGCAGCAG CAGCCGCAGC AGCTGCCACA 
TCGGCAGCGA GTGCTGCGGC AGCGGTGGGC GCACCACCCG TTCCCAACGG ATCGCTGCAG 
CAGTCGCAGC AGCAACAGCA GCAGCAGCAA CAACAGCAGC AGCAACAACA GATGCACATG 
GCGGCCGCGT CGCAACAATT TTTGGCCGCC CAGCAGCAGG CGCAAAATGC GGCCTATGCC 
GCCCAACAGG CCACGTCCTA CGTCATCAAT CCGGGCCAGG AGGCTGCCCC GTATATGGGC 
ATGATTGCCG CCGCCCAGAT GCCGTACTAT GGCGTAGCAC CATGGGGCAT GTATCCGGGC 
AATCTGATTC CGCAACAGGG AACGCAGCCG CGCCGCCCCC TCACCCCCTC GCAGCAGGGT 
GCCGAGAATC AGCCGTATCA GGTCATCCCG GCATTCCTCG ATCACACGGG CTCCTTGCTG 
ATGGGAGGAC CTCGCACCGG GACGCCGATG CGTCTGGTTA GCCCCGCCCC CGTTCTGGTG 
CCCCCGGGCG CTACCCGTGC CGGCCCCCCG CCCCCGCAGG GGCCACAGCT GTATCAGCCG 
CAGCCGCAGA CGGCCCAACA GAATCTCTAC TCGCAGCAGA ATGGATCCAG TGTCGGAGGC 
CTCGCCTTGA ACACGAGCTC GTTGACGGGT CGCCGCGACT CCTTCGACCG CAGCACCTCC 
GCCTTCAGTC CCTCGACCAT GGACTACACC AGCAGCGGTG TGGCAGCGGC CGCCAATGCG 
GTGAACAGCA CAGTGGCCCA GGCAGCAGCA GCTGCCGCAG CAGCCGCCGC AGCGCGTGGC 
AAGTGGCCGG GAGCGATGTC GGGAGCGGCC AGTGGAGCCT ACGGAGCCCT GGGAGCGGGC 
AATGCTTCGG CCAGTCCCCT GGGCGCACCA ATCACGCCGC CGCCATCGGC GCAATCCTGT 
CTCCTGGGCA GTCGGGCACC TGGAGCCGAG TCCCGCCAGC GGCAGCAGCA ACAACAGCAG 
CTGGCCGCCG TTGGTCTGCC GGCGACTGCA GCAGCTGCTC AGGCAGCGGT GGCCGCGGCT 
GCCAACAATA TGTTCGGATC CAACAGCTCG ATCTTCTCGA ATCCCCTGGC CATTCCGGGT 
ACCGCAGCTG TGGCAGCTGC AGCGGCAGCA GCAGCGGCCG CCAACTCGCG TCAGGTGGCT 
GCCACGGCAG CGGCAGCAGC GGCGGTGGCA GCAGCAGCCG GCGGAGTGGG AGGTGCCCCA 
CAGCCAGGAA GATCTCGCCT TCTCGAAGAT TTCCGCAACC AGCGGTATCC AAATCTTCAG 
CTACGCGATC TCGCTAACCA CATTGTGGAG TTCTCACAGG ATCAGCACGG CTCGCGGTTT 
ATCCAACAGA AGTTGGAGCG GGCCACCGCC GCCGAGAAGC AAATGGTGTT CAGCGAGATC 
CTGGCGGCAG CCTATAGCCT GATGACCGAT GTCTTTGGCA ACTATGTCAT CCAGAAGTTC 
TTTGAGTTCG GCACTCCCGA GCAGAAGAAC ACGCTGGGCA TGCAGGTCAA GGGTCATGTG 
CTGCAGCTGG CGCTGCAAAT GTATGGCTGC CGAGTGATTC AGAAGGCTCT GGAGAGCATC 
TCGCCGGAGC AGCAGCAGGA AATCGTCCAC GAACTGGACG GACATGTGCT GAAATGCGTC 
AAGGATCAGA ATGGCAATCA TGTGGTGCAA AAGTGCATTG AGTGCGTGGA CCCCGTGGCG 
CTGCAGTTCA TCATCAATGC TTTCAAGGGT CAGGTTTACT CGCTAAGCAC CCATCCGTAT 
GGATGCCGGG TGATCCAGAG AATCCTTGAG CATTGCACTG CCGAACAGAC CACGCCCATT 
TTGGACGAAC TGCATGAGCA CACCGAACAG TTGATTCAGG ACCAATATGG CAACTATGTT 
ATTCAGCATG TGCTTGAACA CGGCAAGCAG GAGGATAAGT CGATTCTTAT CAACAGCGTG 
CGCGGCAAAG TTCTGGTGCT ATCACAGCAC AAGTTCGCCT CAAACGTTGT GGAGAAATGT 
GTTACCCATG CCACTCGCGG AGAACGCACT GGTCTCATAG ACGAGGTCTG CACCTTCAAC 
GACAACGCGT TGCACGTGAT GATGAAGGAT CAGTATGCCA ACTATGTGGT CCAAAAAATG 
ATCGATGTAT CGGAGCCGAC GCAGCTCAAG AAGCTGATGA CCAAGATCCG GCCCCACATG 
GCCGCCTTGC GCAAGTACAC CTACGGCAAG CACATCAATG CCAAGTTGGA GAAGTACTAC 
ATGAAGATAA CCAATCCCAT TACGGTGGGC ACAGGAGCTG GAGGAGTGCC GGCAGCCTCG 
TCGGCGGCGG CAGTCAGCAG TGGTGCCACC TCGGCATCGG TAACCGCCTG CACCAGTGGC 
AGCAGCACCA CCACGACCAG CACTACCAAC AGCCTGGCCT CACCCACCAT TTGTTCGGTG 
CAGGAGAACG GCAGCGCCAT GGTTGTGGAG CCCTCCTCCC CGGACGCCTC CGAGTCCTCG 
TCCTCGGTGG TGTCAGGCGC TGTCAACAGC AGCTTGGGTC CCATTGGACC CCCGACCAAC 
GGCAACGTTG TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum ( NM_169260.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731316.2 Drosophila melanogaster pumilio (pum), transcript variant B, mRNA.

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CloneID	OFa08171
Clone ID Related Accession (Same CDS sequence)	NM_169260.4
Accession Version	NM_169260.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pumilio, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169260.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAAAT TACTGCACGA CTTCATTTTG GACGCTCGCA CCGCCGAGGA TGTTGCACTC 
ACACAGGAAA TGATTACCAC ATCCACTGCC AAGAAATTGT GGGAGAAATC CGATGGCAAG 
GGCGTATCCT CGAGCACTCC CGGTGGACCG TTGCATCCCC TGCAGATCCC CGGCATCGGG 
GATCCCTCCT CCGTGTGGAA GGATCACACC TGGTCCACAC AGGGCGAGAA TATATTGGTG 
CCGCCCCCCT CGCGAGCCTA CGCCCATGGA GGCGCCTCCG ATACTTCAAA CAGCGGCAAT 
GCGGGCATAC TGAGTCCCCG CGATTCGACT TGCGCCAAAG TGGTTGAATA TGTTTTCAGT 
GGCTCGCCCA CCAACAAAGA TAGCTCGCTT TCCGGATTGG AACCGCATTT GCGGAATCTA 
AAGTTTGACG ACAACGATAA GTCACGCGAC GATAAGGAGA AAGCAAACTC TCCGTTTGAC 
ACAAACGGTT TGAAGAAAGA CGATCAGGTC ACAAACTCAA ATGGTGTTGT CAACGGCATT 
GACGATGACA AGGGCTTCAA TCGCACTCCT GGTTCACGTC AACCATCACC TGCAGAGGAG 
TCCCAGCCAC GTCCCCCCAA TCTACTCTTT CCTCCACTGC CCTTCAATCA CATGCTCATG 
GATCATGGCC AAGGCATGGG AGGCGGCTTG GGCGGAGTTG TTGGATCTGG CAACGGAGTC 
GGCGGTGGCA GCGGCGGAGG CGGGGCAGGC GGCGCTTATG CGGCCCACCA GCAGATGGCC 
GCCCAGATGA GTCAATTGCA ACCGCCGATG ATGAACGGCG TTGGCGGCGG AATGCCAATG 
GCAGCACAGT CACCAATGTT GAATCACCAG GCAGCTGGAC CCAATCACAT GGAATCTCCC 
GGAAATCTCT TGCAGCAGCA AAATTTTGAT GTTCAGCAAC TGTTTCGCTC GCAGAATCCG 
GGCCTAGCAG CAGTTGCCAC AAATGCAGCG GCCGCAGCAG CAGCCGCAGC AGCTGCCACA 
TCGGCAGCGA GTGCTGCGGC AGCGGTGGGC GCACCACCCG TTCCCAACGG ATCGCTGCAG 
CAGTCGCAGC AGCAACAGCA GCAGCAGCAA CAACAGCAGC AGCAACAACA GATGCACATG 
GCGGCCGCGT CGCAACAATT TTTGGCCGCC CAGCAGCAGG CGCAAAATGC GGCCTATGCC 
GCCCAACAGG CCACGTCCTA CGTCATCAAT CCGGGCCAGG AGGCTGCCCC GTATATGGGC 
ATGATTGCCG CCGCCCAGAT GCCGTACTAT GGCGTAGCAC CATGGGGCAT GTATCCGGGC 
AATCTGATTC CGCAACAGGG AACGCAGCCG CGCCGCCCCC TCACCCCCTC GCAGCAGGGT 
GCCGAGAATC AGCCGTATCA GGTCATCCCG GCATTCCTCG ATCACACGGG CTCCTTGCTG 
ATGGGAGGAC CTCGCACCGG GACGCCGATG CGTCTGGTTA GCCCCGCCCC CGTTCTGGTG 
CCCCCGGGCG CTACCCGTGC CGGCCCCCCG CCCCCGCAGG GGCCACAGCT GTATCAGCCG 
CAGCCGCAGA CGGCCCAACA GAATCTCTAC TCGCAGCAGA ATGGATCCAG TGTCGGAGGC 
CTCGCCTTGA ACACGAGCTC GTTGACGGGT CGCCGCGACT CCTTCGACCG CAGCACCTCC 
GCCTTCAGTC CCTCGACCAT GGACTACACC AGCAGCGGTG TGGCAGCGGC CGCCAATGCG 
GTGAACAGCA CAGTGGCCCA GGCAGCAGCA GCTGCCGCAG CAGCCGCCGC AGCGCGTGGC 
AAGTGGCCGG GAGCGATGTC GGGAGCGGCC AGTGGAGCCT ACGGAGCCCT GGGAGCGGGC 
AATGCTTCGG CCAGTCCCCT GGGCGCACCA ATCACGCCGC CGCCATCGGC GCAATCCTGT 
CTCCTGGGCA GTCGGGCACC TGGAGCCGAG TCCCGCCAGC GGCAGCAGCA ACAACAGCAG 
CTGGCCGCCG TTGGTCTGCC GGCGACTGCA GCAGCTGCTC AGGCAGCGGT GGCCGCGGCT 
GCCAACAATA TGTTCGGATC CAACAGCTCG ATCTTCTCGA ATCCCCTGGC CATTCCGGGT 
ACCGCAGCTG TGGCAGCTGC AGCGGCAGCA GCAGCGGCCG CCAACTCGCG TCAGGTGGCT 
GCCACGGCAG CGGCAGCAGC GGCGGTGGCA GCAGCAGCCG GCGGAGTGGG AGGTGCCCCA 
CAGCCAGGAA GATCTCGCCT TCTCGAAGAT TTCCGCAACC AGCGGTATCC AAATCTTCAG 
CTACGCGATC TCGCTAACCA CATTGTGGAG TTCTCACAGG ATCAGCACGG CTCGCGGTTT 
ATCCAACAGA AGTTGGAGCG GGCCACCGCC GCCGAGAAGC AAATGGTGTT CAGCGAGATC 
CTGGCGGCAG CCTATAGCCT GATGACCGAT GTCTTTGGCA ACTATGTCAT CCAGAAGTTC 
TTTGAGTTCG GCACTCCCGA GCAGAAGAAC ACGCTGGGCA TGCAGGTCAA GGGTCATGTG 
CTGCAGCTGG CGCTGCAAAT GTATGGCTGC CGAGTGATTC AGAAGGCTCT GGAGAGCATC 
TCGCCGGAGC AGCAGCAGGA AATCGTCCAC GAACTGGACG GACATGTGCT GAAATGCGTC 
AAGGATCAGA ATGGCAATCA TGTGGTGCAA AAGTGCATTG AGTGCGTGGA CCCCGTGGCG 
CTGCAGTTCA TCATCAATGC TTTCAAGGGT CAGGTTTACT CGCTAAGCAC CCATCCGTAT 
GGATGCCGGG TGATCCAGAG AATCCTTGAG CATTGCACTG CCGAACAGAC CACGCCCATT 
TTGGACGAAC TGCATGAGCA CACCGAACAG TTGATTCAGG ACCAATATGG CAACTATGTT 
ATTCAGCATG TGCTTGAACA CGGCAAGCAG GAGGATAAGT CGATTCTTAT CAACAGCGTG 
CGCGGCAAAG TTCTGGTGCT ATCACAGCAC AAGTTCGCCT CAAACGTTGT GGAGAAATGT 
GTTACCCATG CCACTCGCGG AGAACGCACT GGTCTCATAG ACGAGGTCTG CACCTTCAAC 
GACAACGCGT TGCACGTGAT GATGAAGGAT CAGTATGCCA ACTATGTGGT CCAAAAAATG 
ATCGATGTAT CGGAGCCGAC GCAGCTCAAG AAGCTGATGA CCAAGATCCG GCCCCACATG 
GCCGCCTTGC GCAAGTACAC CTACGGCAAG CACATCAATG CCAAGTTGGA GAAGTACTAC 
ATGAAGATAA CCAATCCCAT TACGGTGGGC ACAGGAGCTG GAGGAGTGCC GGCAGCCTCG 
TCGGCGGCGG CAGTCAGCAG TGGTGCCACC TCGGCATCGG TAACCGCCTG CACCAGTGGC 
AGCAGCACCA CCACGACCAG CACTACCAAC AGCCTGGCCT CACCCACCAT TTGTTCGGTG 
CAGGAGAACG GCAGCGCCAT GGTTGTGGAG CCCTCCTCCC CGGACGCCTC CGAGTCCTCG 
TCCTCGGTGG TGTCAGGCGC TGTCAACAGC AGCTTGGGTC CCATTGGACC CCCGACCAAC 
GGCAACGTTG TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731316.2
CDS	549..4106
Translation	MMKLLHDFILDARTAEDVALTQEMITTSTAKKLWEKSDGKGVSSSTPGGPLHPLQIPGIGDPSSVWKDHTWSTQGENILVPPPSRAYAHGGASDTSNSGNAGILSPRDSTCAKVVEYVFSGSPTNKDSSLSGLEPHLRNLKFDDNDKSRDDKEKANSPFDTNGLKKDDQVTNSNGVVNGIDDDKGFNRTPGSRQPSPAEESQPRPPNLLFPPLPFNHMLMDHGQGMGGGLGGVVGSGNGVGGGSGGGGAGGAYAAHQQMAAQMSQLQPPMMNGVGGGMPMAAQSPMLNHQAAGPNHMESPGNLLQQQNFDVQQLFRSQNPGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQQQQQQMHMAAASQQFLAAQQQAQNAAYAAQQATSYVINPGQEAAPYMGMIAAAQMPYYGVAPWGMYPGNLIPQQGTQPRRPLTPSQQGAENQPYQVIPAFLDHTGSLLMGGPRTGTPMRLVSPAPVLVPPGATRAGPPPPQGPQLYQPQPQTAQQNLYSQQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPSTMDYTSSGVAAAANAVNSTVAQAAAAAAAAAAARGKWPGAMSGAASGAYGALGAGNASASPLGAPITPPPSAQSCLLGSRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCTFNDNALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLEKYYMKITNPITVGTGAGGVPAASSAAAVSSGATSASVTACTSGSSTTTTSTTNSLASPTICSVQENGSAMVVEPSSPDASESSSSVVSGAVNSSLGPIGPPTNGNVVL

Target ORF information:

RefSeq Version	NM_169260.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pumilio (pum), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169260.4

ORF Insert Sequence:

ATGATGAAAT TACTGCACGA CTTCATTTTG GACGCTCGCA CCGCCGAGGA TGTTGCACTC 
ACACAGGAAA TGATTACCAC ATCCACTGCC AAGAAATTGT GGGAGAAATC CGATGGCAAG 
GGCGTATCCT CGAGCACTCC CGGTGGACCG TTGCATCCCC TGCAGATCCC CGGCATCGGG 
GATCCCTCCT CCGTGTGGAA GGATCACACC TGGTCCACAC AGGGCGAGAA TATATTGGTG 
CCGCCCCCCT CGCGAGCCTA CGCCCATGGA GGCGCCTCCG ATACTTCAAA CAGCGGCAAT 
GCGGGCATAC TGAGTCCCCG CGATTCGACT TGCGCCAAAG TGGTTGAATA TGTTTTCAGT 
GGCTCGCCCA CCAACAAAGA TAGCTCGCTT TCCGGATTGG AACCGCATTT GCGGAATCTA 
AAGTTTGACG ACAACGATAA GTCACGCGAC GATAAGGAGA AAGCAAACTC TCCGTTTGAC 
ACAAACGGTT TGAAGAAAGA CGATCAGGTC ACAAACTCAA ATGGTGTTGT CAACGGCATT 
GACGATGACA AGGGCTTCAA TCGCACTCCT GGTTCACGTC AACCATCACC TGCAGAGGAG 
TCCCAGCCAC GTCCCCCCAA TCTACTCTTT CCTCCACTGC CCTTCAATCA CATGCTCATG 
GATCATGGCC AAGGCATGGG AGGCGGCTTG GGCGGAGTTG TTGGATCTGG CAACGGAGTC 
GGCGGTGGCA GCGGCGGAGG CGGGGCAGGC GGCGCTTATG CGGCCCACCA GCAGATGGCC 
GCCCAGATGA GTCAATTGCA ACCGCCGATG ATGAACGGCG TTGGCGGCGG AATGCCAATG 
GCAGCACAGT CACCAATGTT GAATCACCAG GCAGCTGGAC CCAATCACAT GGAATCTCCC 
GGAAATCTCT TGCAGCAGCA AAATTTTGAT GTTCAGCAAC TGTTTCGCTC GCAGAATCCG 
GGCCTAGCAG CAGTTGCCAC AAATGCAGCG GCCGCAGCAG CAGCCGCAGC AGCTGCCACA 
TCGGCAGCGA GTGCTGCGGC AGCGGTGGGC GCACCACCCG TTCCCAACGG ATCGCTGCAG 
CAGTCGCAGC AGCAACAGCA GCAGCAGCAA CAACAGCAGC AGCAACAACA GATGCACATG 
GCGGCCGCGT CGCAACAATT TTTGGCCGCC CAGCAGCAGG CGCAAAATGC GGCCTATGCC 
GCCCAACAGG CCACGTCCTA CGTCATCAAT CCGGGCCAGG AGGCTGCCCC GTATATGGGC 
ATGATTGCCG CCGCCCAGAT GCCGTACTAT GGCGTAGCAC CATGGGGCAT GTATCCGGGC 
AATCTGATTC CGCAACAGGG AACGCAGCCG CGCCGCCCCC TCACCCCCTC GCAGCAGGGT 
GCCGAGAATC AGCCGTATCA GGTCATCCCG GCATTCCTCG ATCACACGGG CTCCTTGCTG 
ATGGGAGGAC CTCGCACCGG GACGCCGATG CGTCTGGTTA GCCCCGCCCC CGTTCTGGTG 
CCCCCGGGCG CTACCCGTGC CGGCCCCCCG CCCCCGCAGG GGCCACAGCT GTATCAGCCG 
CAGCCGCAGA CGGCCCAACA GAATCTCTAC TCGCAGCAGA ATGGATCCAG TGTCGGAGGC 
CTCGCCTTGA ACACGAGCTC GTTGACGGGT CGCCGCGACT CCTTCGACCG CAGCACCTCC 
GCCTTCAGTC CCTCGACCAT GGACTACACC AGCAGCGGTG TGGCAGCGGC CGCCAATGCG 
GTGAACAGCA CAGTGGCCCA GGCAGCAGCA GCTGCCGCAG CAGCCGCCGC AGCGCGTGGC 
AAGTGGCCGG GAGCGATGTC GGGAGCGGCC AGTGGAGCCT ACGGAGCCCT GGGAGCGGGC 
AATGCTTCGG CCAGTCCCCT GGGCGCACCA ATCACGCCGC CGCCATCGGC GCAATCCTGT 
CTCCTGGGCA GTCGGGCACC TGGAGCCGAG TCCCGCCAGC GGCAGCAGCA ACAACAGCAG 
CTGGCCGCCG TTGGTCTGCC GGCGACTGCA GCAGCTGCTC AGGCAGCGGT GGCCGCGGCT 
GCCAACAATA TGTTCGGATC CAACAGCTCG ATCTTCTCGA ATCCCCTGGC CATTCCGGGT 
ACCGCAGCTG TGGCAGCTGC AGCGGCAGCA GCAGCGGCCG CCAACTCGCG TCAGGTGGCT 
GCCACGGCAG CGGCAGCAGC GGCGGTGGCA GCAGCAGCCG GCGGAGTGGG AGGTGCCCCA 
CAGCCAGGAA GATCTCGCCT TCTCGAAGAT TTCCGCAACC AGCGGTATCC AAATCTTCAG 
CTACGCGATC TCGCTAACCA CATTGTGGAG TTCTCACAGG ATCAGCACGG CTCGCGGTTT 
ATCCAACAGA AGTTGGAGCG GGCCACCGCC GCCGAGAAGC AAATGGTGTT CAGCGAGATC 
CTGGCGGCAG CCTATAGCCT GATGACCGAT GTCTTTGGCA ACTATGTCAT CCAGAAGTTC 
TTTGAGTTCG GCACTCCCGA GCAGAAGAAC ACGCTGGGCA TGCAGGTCAA GGGTCATGTG 
CTGCAGCTGG CGCTGCAAAT GTATGGCTGC CGAGTGATTC AGAAGGCTCT GGAGAGCATC 
TCGCCGGAGC AGCAGCAGGA AATCGTCCAC GAACTGGACG GACATGTGCT GAAATGCGTC 
AAGGATCAGA ATGGCAATCA TGTGGTGCAA AAGTGCATTG AGTGCGTGGA CCCCGTGGCG 
CTGCAGTTCA TCATCAATGC TTTCAAGGGT CAGGTTTACT CGCTAAGCAC CCATCCGTAT 
GGATGCCGGG TGATCCAGAG AATCCTTGAG CATTGCACTG CCGAACAGAC CACGCCCATT 
TTGGACGAAC TGCATGAGCA CACCGAACAG TTGATTCAGG ACCAATATGG CAACTATGTT 
ATTCAGCATG TGCTTGAACA CGGCAAGCAG GAGGATAAGT CGATTCTTAT CAACAGCGTG 
CGCGGCAAAG TTCTGGTGCT ATCACAGCAC AAGTTCGCCT CAAACGTTGT GGAGAAATGT 
GTTACCCATG CCACTCGCGG AGAACGCACT GGTCTCATAG ACGAGGTCTG CACCTTCAAC 
GACAACGCGT TGCACGTGAT GATGAAGGAT CAGTATGCCA ACTATGTGGT CCAAAAAATG 
ATCGATGTAT CGGAGCCGAC GCAGCTCAAG AAGCTGATGA CCAAGATCCG GCCCCACATG 
GCCGCCTTGC GCAAGTACAC CTACGGCAAG CACATCAATG CCAAGTTGGA GAAGTACTAC 
ATGAAGATAA CCAATCCCAT TACGGTGGGC ACAGGAGCTG GAGGAGTGCC GGCAGCCTCG 
TCGGCGGCGG CAGTCAGCAG TGGTGCCACC TCGGCATCGG TAACCGCCTG CACCAGTGGC 
AGCAGCACCA CCACGACCAG CACTACCAAC AGCCTGGCCT CACCCACCAT TTGTTCGGTG 
CAGGAGAACG GCAGCGCCAT GGTTGTGGAG CCCTCCTCCC CGGACGCCTC CGAGTCCTCG 
TCCTCGGTGG TGTCAGGCGC TGTCAACAGC AGCTTGGGTC CCATTGGACC CCCGACCAAC 
GGCAACGTTG TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum ( NM_176427.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788604.1 Drosophila melanogaster pumilio (pum), transcript variant E, mRNA.

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CloneID	OFa08172
Clone ID Related Accession (Same CDS sequence)	NM_176427.3
Accession Version	NM_176427.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pumilio, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176427.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGTTT TAGAAACTGC CAGCGCCCTT TTGGGCGGCC CGTATGCCCA GGGCGCTCCC 
GCCCTGAAAA TGGTGCAGAA ACGCTACATT GGATTACATC ACTGGCTGGG ACCAATCCGC 
TCCAAGGAGC TAAAGGAGCA TATTGTAAGT GACGACGTGC TTTCGCTCGC TTTTAACCAC 
AATCAACAAC TGTTTCGCTC GCAGAATCCG GGCCTAGCAG CAGTTGCCAC AAATGCAGCG 
GCCGCAGCAG CAGCCGCAGC AGCTGCCACA TCGGCAGCGA GTGCTGCGGC AGCGGTGGGC 
GCACCACCCG TTCCCAACGG ATCGCTGCAG CAGTCGCAGC AGCAACAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA GATGCACATG GCGGCCGCGT CGCAACAATT TTTGGCCGCC 
CAGCAGCAGG CGCAAAATGC GGCCTATGCC GCCCAACAGG CCACGTCCTA CGTCATCAAT 
CCGGGCCAGG AGGCTGCCCC GTATATGGGC ATGATTGCCG CCGCCCAGAT GCCGTACTAT 
GGCGTAGCAC CATGGGGCAT GTATCCGGGC AATCTGATTC CGCAACAGGG AACGCAGCCG 
CGCCGCCCCC TCACCCCCTC GCAGCAGGGT GCCGAGAATC AGCCGTATCA GGTCATCCCG 
GCATTCCTCG ATCACACGGG CTCCTTGCTG ATGGGAGGAC CTCGCACCGG GACGCCGATG 
CGTCTGGTTA GCCCCGCCCC CGTTCTGGTG CCCCCGGGCG CTACCCGTGC CGGCCCCCCG 
CCCCCGCAGG GGCCACAGCT GTATCAGCCG CAGCCGCAGA CGGCCCAACA GAATCTCTAC 
TCGCAGCAGA ATGGATCCAG TGTCGGAGGC CTCGCCTTGA ACACGAGCTC GTTGACGGGT 
CGCCGCGACT CCTTCGACCG CAGCACCTCC GCCTTCAGTC CCTCGACCAT GGACTACACC 
AGCAGCGGTG TGGCAGCGGC CGCCAATGCG GTGAACAGCA CAGTGGCCCA GGCAGCAGCA 
GCTGCCGCAG CAGCCGCCGC AGCGCGTGGC AAGTGGCCGG GAGCGATGTC GGGAGCGGCC 
AGTGGAGCCT ACGGAGCCCT GGGAGCGGGC AATGCTTCGG CCAGTCCCCT GGGCGCACCA 
ATCACGCCGC CGCCATCGGC GCAATCCTGT CTCCTGGGCA GTCGGGCACC TGGAGCCGAG 
TCCCGCCAGC GGCAGCAGCA ACAACAGCAG CTGGCCGCCG TTGGTCTGCC GGCGACTGCA 
GCAGCTGCTC AGGCAGCGGT GGCCGCGGCT GCCAACAATA TGTTCGGATC CAACAGCTCG 
ATCTTCTCGA ATCCCCTGGC CATTCCGGGT ACCGCAGCTG TGGCAGCTGC AGCGGCAGCA 
GCAGCGGCCG CCAACTCGCG TCAGGTGGCT GCCACGGCAG CGGCAGCAGC GGCGGTGGCA 
GCAGCAGCCG GCGGAGTGGG AGGTGCCCCA CAGCCAGGAA GATCTCGCCT TCTCGAAGAT 
TTCCGCAACC AGCGGTATCC AAATCTTCAG CTACGCGATC TCGCTAACCA CATTGTGGAG 
TTCTCACAGG ATCAGCACGG CTCGCGGTTT ATCCAACAGA AGTTGGAGCG GGCCACCGCC 
GCCGAGAAGC AAATGGTGTT CAGCGAGATC CTGGCGGCAG CCTATAGCCT GATGACCGAT 
GTCTTTGGCA ACTATGTCAT CCAGAAGTTC TTTGAGTTCG GCACTCCCGA GCAGAAGAAC 
ACGCTGGGCA TGCAGGTCAA GGGTCATGTG CTGCAGCTGG CGCTGCAAAT GTATGGCTGC 
CGAGTGATTC AGAAGGCTCT GGAGAGCATC TCGCCGGAGC AGCAGCAGGA AATCGTCCAC 
GAACTGGACG GACATGTGCT GAAATGCGTC AAGGATCAGA ATGGCAATCA TGTGGTGCAA 
AAGTGCATTG AGTGCGTGGA CCCCGTGGCG CTGCAGTTCA TCATCAATGC TTTCAAGGGT 
CAGGTTTACT CGCTAAGCAC CCATCCGTAT GGATGCCGGG TGATCCAGAG AATCCTTGAG 
CATTGCACTG CCGAACAGAC CACGCCCATT TTGGACGAAC TGCATGAGCA CACCGAACAG 
TTGATTCAGG ACCAATATGG CAACTATGTT ATTCAGCATG TGCTTGAACA CGGCAAGCAG 
GAGGATAAGT CGATTCTTAT CAACAGCGTG CGCGGCAAAG TTCTGGTGCT ATCACAGCAC 
AAGTTCGCCT CAAACGTTGT GGAGAAATGT GTTACCCATG CCACTCGCGG AGAACGCACT 
GGTCTCATAG ACGAGGTCTG CACCTTCAAC GACAACGCGT TGCACGTGAT GATGAAGGAT 
CAGTATGCCA ACTATGTGGT CCAAAAAATG ATCGATGTAT CGGAGCCGAC GCAGCTCAAG 
AAGCTGATGA CCAAGATCCG GCCCCACATG GCCGCCTTGC GCAAGTACAC CTACGGCAAG 
CACATCAATG CCAAGTTGGA GAAGTACTAC ATGAAGATAA CCAATCCCAT TACGGTGGGC 
ACAGGAGCTG GAGGAGTGCC GGCAGCCTCG TCGGCGGCGG CAGTCAGCAG TGGTGCCACC 
TCGGCATCGG TAACCGCCTG CACCAGTGGC AGCAGCACCA CCACGACCAG CACTACCAAC 
AGCCTGGCCT CACCCACCAT TTGTTCGGTG CAGGAGAACG GCAGCGCCAT GGTTGTGGAG 
CCCTCCTCCC CGGACGCCTC CGAGTCCTCG TCCTCGGTGG TGTCAGGCGC TGTCAACAGC 
AGCTTGGGTC CCATTGGACC CCCGACCAAC GGCAACGTTG TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788604.1
CDS	690..3497
Translation	MVVLETASALLGGPYAQGAPALKMVQKRYIGLHHWLGPIRSKELKEHIVSDDVLSLAFNHNQQLFRSQNPGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQQQQQQMHMAAASQQFLAAQQQAQNAAYAAQQATSYVINPGQEAAPYMGMIAAAQMPYYGVAPWGMYPGNLIPQQGTQPRRPLTPSQQGAENQPYQVIPAFLDHTGSLLMGGPRTGTPMRLVSPAPVLVPPGATRAGPPPPQGPQLYQPQPQTAQQNLYSQQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPSTMDYTSSGVAAAANAVNSTVAQAAAAAAAAAAARGKWPGAMSGAASGAYGALGAGNASASPLGAPITPPPSAQSCLLGSRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCTFNDNALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLEKYYMKITNPITVGTGAGGVPAASSAAAVSSGATSASVTACTSGSSTTTTSTTNSLASPTICSVQENGSAMVVEPSSPDASESSSSVVSGAVNSSLGPIGPPTNGNVVL

Target ORF information:

RefSeq Version	NM_176427.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pumilio (pum), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176427.3

ORF Insert Sequence:

ATGGTGGTTT TAGAAACTGC CAGCGCCCTT TTGGGCGGCC CGTATGCCCA GGGCGCTCCC 
GCCCTGAAAA TGGTGCAGAA ACGCTACATT GGATTACATC ACTGGCTGGG ACCAATCCGC 
TCCAAGGAGC TAAAGGAGCA TATTGTAAGT GACGACGTGC TTTCGCTCGC TTTTAACCAC 
AATCAACAAC TGTTTCGCTC GCAGAATCCG GGCCTAGCAG CAGTTGCCAC AAATGCAGCG 
GCCGCAGCAG CAGCCGCAGC AGCTGCCACA TCGGCAGCGA GTGCTGCGGC AGCGGTGGGC 
GCACCACCCG TTCCCAACGG ATCGCTGCAG CAGTCGCAGC AGCAACAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA GATGCACATG GCGGCCGCGT CGCAACAATT TTTGGCCGCC 
CAGCAGCAGG CGCAAAATGC GGCCTATGCC GCCCAACAGG CCACGTCCTA CGTCATCAAT 
CCGGGCCAGG AGGCTGCCCC GTATATGGGC ATGATTGCCG CCGCCCAGAT GCCGTACTAT 
GGCGTAGCAC CATGGGGCAT GTATCCGGGC AATCTGATTC CGCAACAGGG AACGCAGCCG 
CGCCGCCCCC TCACCCCCTC GCAGCAGGGT GCCGAGAATC AGCCGTATCA GGTCATCCCG 
GCATTCCTCG ATCACACGGG CTCCTTGCTG ATGGGAGGAC CTCGCACCGG GACGCCGATG 
CGTCTGGTTA GCCCCGCCCC CGTTCTGGTG CCCCCGGGCG CTACCCGTGC CGGCCCCCCG 
CCCCCGCAGG GGCCACAGCT GTATCAGCCG CAGCCGCAGA CGGCCCAACA GAATCTCTAC 
TCGCAGCAGA ATGGATCCAG TGTCGGAGGC CTCGCCTTGA ACACGAGCTC GTTGACGGGT 
CGCCGCGACT CCTTCGACCG CAGCACCTCC GCCTTCAGTC CCTCGACCAT GGACTACACC 
AGCAGCGGTG TGGCAGCGGC CGCCAATGCG GTGAACAGCA CAGTGGCCCA GGCAGCAGCA 
GCTGCCGCAG CAGCCGCCGC AGCGCGTGGC AAGTGGCCGG GAGCGATGTC GGGAGCGGCC 
AGTGGAGCCT ACGGAGCCCT GGGAGCGGGC AATGCTTCGG CCAGTCCCCT GGGCGCACCA 
ATCACGCCGC CGCCATCGGC GCAATCCTGT CTCCTGGGCA GTCGGGCACC TGGAGCCGAG 
TCCCGCCAGC GGCAGCAGCA ACAACAGCAG CTGGCCGCCG TTGGTCTGCC GGCGACTGCA 
GCAGCTGCTC AGGCAGCGGT GGCCGCGGCT GCCAACAATA TGTTCGGATC CAACAGCTCG 
ATCTTCTCGA ATCCCCTGGC CATTCCGGGT ACCGCAGCTG TGGCAGCTGC AGCGGCAGCA 
GCAGCGGCCG CCAACTCGCG TCAGGTGGCT GCCACGGCAG CGGCAGCAGC GGCGGTGGCA 
GCAGCAGCCG GCGGAGTGGG AGGTGCCCCA CAGCCAGGAA GATCTCGCCT TCTCGAAGAT 
TTCCGCAACC AGCGGTATCC AAATCTTCAG CTACGCGATC TCGCTAACCA CATTGTGGAG 
TTCTCACAGG ATCAGCACGG CTCGCGGTTT ATCCAACAGA AGTTGGAGCG GGCCACCGCC 
GCCGAGAAGC AAATGGTGTT CAGCGAGATC CTGGCGGCAG CCTATAGCCT GATGACCGAT 
GTCTTTGGCA ACTATGTCAT CCAGAAGTTC TTTGAGTTCG GCACTCCCGA GCAGAAGAAC 
ACGCTGGGCA TGCAGGTCAA GGGTCATGTG CTGCAGCTGG CGCTGCAAAT GTATGGCTGC 
CGAGTGATTC AGAAGGCTCT GGAGAGCATC TCGCCGGAGC AGCAGCAGGA AATCGTCCAC 
GAACTGGACG GACATGTGCT GAAATGCGTC AAGGATCAGA ATGGCAATCA TGTGGTGCAA 
AAGTGCATTG AGTGCGTGGA CCCCGTGGCG CTGCAGTTCA TCATCAATGC TTTCAAGGGT 
CAGGTTTACT CGCTAAGCAC CCATCCGTAT GGATGCCGGG TGATCCAGAG AATCCTTGAG 
CATTGCACTG CCGAACAGAC CACGCCCATT TTGGACGAAC TGCATGAGCA CACCGAACAG 
TTGATTCAGG ACCAATATGG CAACTATGTT ATTCAGCATG TGCTTGAACA CGGCAAGCAG 
GAGGATAAGT CGATTCTTAT CAACAGCGTG CGCGGCAAAG TTCTGGTGCT ATCACAGCAC 
AAGTTCGCCT CAAACGTTGT GGAGAAATGT GTTACCCATG CCACTCGCGG AGAACGCACT 
GGTCTCATAG ACGAGGTCTG CACCTTCAAC GACAACGCGT TGCACGTGAT GATGAAGGAT 
CAGTATGCCA ACTATGTGGT CCAAAAAATG ATCGATGTAT CGGAGCCGAC GCAGCTCAAG 
AAGCTGATGA CCAAGATCCG GCCCCACATG GCCGCCTTGC GCAAGTACAC CTACGGCAAG 
CACATCAATG CCAAGTTGGA GAAGTACTAC ATGAAGATAA CCAATCCCAT TACGGTGGGC 
ACAGGAGCTG GAGGAGTGCC GGCAGCCTCG TCGGCGGCGG CAGTCAGCAG TGGTGCCACC 
TCGGCATCGG TAACCGCCTG CACCAGTGGC AGCAGCACCA CCACGACCAG CACTACCAAC 
AGCCTGGCCT CACCCACCAT TTGTTCGGTG CAGGAGAACG GCAGCGCCAT GGTTGTGGAG 
CCCTCCTCCC CGGACGCCTC CGAGTCCTCG TCCTCGGTGG TGTCAGGCGC TGTCAACAGC 
AGCTTGGGTC CCATTGGACC CCCGACCAAC GGCAACGTTG TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pum cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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