stck cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	stck
Entrez Gene ID	40999
Full Name	steamer duck
Synonyms	CG7954,D-PINCH,Dmel\CG7954,PINCH,Pinch,dPINCH,dpin,pin85A,pinch,stk
General protein information	Preferred Names steamer duck Names CG7954 gene product from transcript CG7954-RD CG7954-PA CG7954-PB CG7954-PC CG7954-PD PINCH d-pinch stck-PA stck-PB stck-PC stck-PD steamerduck
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84F15-84F15

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260058.2	NP_001246987.1	steamer duck, isoform D
NM_169220.3	NP_731241.1	steamer duck, isoform B
NM_079554.4	NP_524278.1	steamer duck, isoform A
NM_169221.3	NP_731242.1	steamer duck, isoform C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LIMS1	XP_005626038.1
Mus musculus (house mouse)	Lims1	NP_957694.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003429	XP_003436478.1
	LIMS1	XP_002799414.1
Bos taurus (cattle)	LIMS1	NP_001076964.1
Rattus norvegicus (Norway rat)	Lims1	NP_001138928.1
Gallus gallus (chicken)	LIMS1	NP_001001766.1
Drosophila melanogaster (fruit fly)	stck	NP_731242.1
Xenopus tropicalis (tropical clawed frog)	lims1	NP_001135570.1
Pan troglodytes (chimpanzee)	LIMS1	XP_001136802.1
Caenorhabditis elegans (roundworm)	unc-97	NP_508943.3
Homo sapiens (human)	LIMS1	NP_001180412.1
Danio rerio (zebrafish)	lims1	NP_001019560.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Targeted knockdown of the integrin focal adhesion complex components beta-integrin, PINCH, and integrin-linked kinase caused formation of multinucleate epidermal cells within the Drosophila larval epidermis.
Title: Integrin Adhesions Suppress Syncytium Formation in the Drosophila Larval Epidermis.

By testing a panel of IAC proteins, we show specificity for PINCH expression in the rescue of hypercontraction mutants
Title: Elevated expression of the integrin-associated protein PINCH suppresses the defects of Drosophila melanogaster muscle hypercontraction mutants.

PINCH and ILK have an independent capacity to localize at muscle attachment sites in vivo.
Title: A crucial role for Ras suppressor-1 (RSU-1) revealed when PINCH and ILK binding is disrupted.

Both PINCH and RSU-1 antagonize JNK signaling during Drosophila embryonic dorsal closure, suggesting both PINCH and RSU-1 contribute to the integration of JNK and integrin functions during Drosophila development
Title: The integrin effector PINCH regulates JNK activity and epithelial migration in concert with Ras suppressor 1.

The following stck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the stck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04247	NM_001260058.2 Latest version!	Drosophila melanogaster steamer duck (stck), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04247	NM_079554.4 Latest version!	Drosophila melanogaster steamer duck (stck), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08143	NM_169220.3 Latest version!	Drosophila melanogaster steamer duck (stck), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08144	NM_169221.3 Latest version!	Drosophila melanogaster steamer duck (stck), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

stck ( NM_001260058.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246987.1 Drosophila melanogaster steamer duck (stck), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa04247
Clone ID Related Accession (Same CDS sequence)	NM_001260058.2 , NM_079554.4
Accession Version	NM_001260058.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	steamer duck, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260058.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTGG GTGCCATGCA TTGTACGCGA TGTGCCGACG GCTTCGAGCC GACTGAGAAG 
ATTGTTAACT CCAATGGAGA ACTCTGGCAC ACCCAGTGCT TCGTATGTGC CCAGTGCTTC 
CGACCCTTCC AGGATGGGAT ATTCTACGAG TTCGAGGGCC GTAAGTACTG TGAACGCGAC 
TTTCACGTGC TCTTCGCTCC CTGCTGCAAC AAGTGCGGCG AGTTCGTCAT CGGACGGGTC 
ATCAAGGCCA TGTCAGCTAG CTGGCATCCA CAGTGCTTCC GCTGCCAATT GTGCGCCAAG 
GAGCTGGCTG ATTGCGGATT CATCAAGAAC CAGAACCGCG CCTTGTGTCA CGAGTGCAAT 
GCCAAGGTGA AGGCGGAGAT TACCGGCCGC TATGTATGCC AAAAATGCCA CGGCCTCATC 
GACGAGGAGC CTTTGCGATT CCGGGGCGAA GTGTACCACG GCTATCACTT CAGTTGCACG 
GCCTGCGGCA CAGAGTTGGA TTCCACTGCT CGGGAGGTCA AGAGCCGGCC AGGATTGGCT 
GCTAACGATA TGAACGAACT GTATTGTCTG CGTTGTCACG ATAAAATGGG CATTCCCATA 
TGTGGTGCCT GTCGTCGTCC GATTGAGGAG AGGGTGGTAA CGGCGCTCGG AAAGCACTGG 
CATGTGGAAC ATTTCGTGTG CGCCAAGTGC GAGAAACCCT TCTTGGGTCA CCGACACTAC 
GAGAAGCGGG GATTGGCCTA CTGCGAGACG CACTATCATC AGCTCTTTGG CAACCTGTGC 
TTCGTCTGCA ACCAGGTTAT TGGAGGCGAT GTTTTCACTG CCTTGAACAA GGCCTGGTGT 
GTGCACCACT TTGCGTGCTC CGTGTGCGAC ACGAAGATGA CACAAAAATC GAAATTCTAC 
GAGTACGACG AGAAGCCGGT GTGCAAAAAG TGCTATGACC GATTTCCAAA CGAACTCAGA 
CGACGTCTGC GCACCGCCCA CGAGATGACA ATGAAAAAGA ACACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246987.1
CDS	255..1262
Translation	MSLGAMHCTRCADGFEPTEKIVNSNGELWHTQCFVCAQCFRPFQDGIFYEFEGRKYCERDFHVLFAPCCNKCGEFVIGRVIKAMSASWHPQCFRCQLCAKELADCGFIKNQNRALCHECNAKVKAEITGRYVCQKCHGLIDEEPLRFRGEVYHGYHFSCTACGTELDSTAREVKSRPGLAANDMNELYCLRCHDKMGIPICGACRRPIEERVVTALGKHWHVEHFVCAKCEKPFLGHRHYEKRGLAYCETHYHQLFGNLCFVCNQVIGGDVFTALNKAWCVHHFACSVCDTKMTQKSKFYEYDEKPVCKKCYDRFPNELRRRLRTAHEMTMKKNT

Target ORF information:

RefSeq Version	NM_001260058.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster steamer duck (stck), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260058.2

ORF Insert Sequence:

ATGTCGCTGG GTGCCATGCA TTGTACGCGA TGTGCCGACG GCTTCGAGCC GACTGAGAAG 
ATTGTTAACT CCAATGGAGA ACTCTGGCAC ACCCAGTGCT TCGTATGTGC CCAGTGCTTC 
CGACCCTTCC AGGATGGGAT ATTCTACGAG TTCGAGGGCC GTAAGTACTG TGAACGCGAC 
TTTCACGTGC TCTTCGCTCC CTGCTGCAAC AAGTGCGGCG AGTTCGTCAT CGGACGGGTC 
ATCAAGGCCA TGTCAGCTAG CTGGCATCCA CAGTGCTTCC GCTGCCAATT GTGCGCCAAG 
GAGCTGGCTG ATTGCGGATT CATCAAGAAC CAGAACCGCG CCTTGTGTCA CGAGTGCAAT 
GCCAAGGTGA AGGCGGAGAT TACCGGCCGC TATGTATGCC AAAAATGCCA CGGCCTCATC 
GACGAGGAGC CTTTGCGATT CCGGGGCGAA GTGTACCACG GCTATCACTT CAGTTGCACG 
GCCTGCGGCA CAGAGTTGGA TTCCACTGCT CGGGAGGTCA AGAGCCGGCC AGGATTGGCT 
GCTAACGATA TGAACGAACT GTATTGTCTG CGTTGTCACG ATAAAATGGG CATTCCCATA 
TGTGGTGCCT GTCGTCGTCC GATTGAGGAG AGGGTGGTAA CGGCGCTCGG AAAGCACTGG 
CATGTGGAAC ATTTCGTGTG CGCCAAGTGC GAGAAACCCT TCTTGGGTCA CCGACACTAC 
GAGAAGCGGG GATTGGCCTA CTGCGAGACG CACTATCATC AGCTCTTTGG CAACCTGTGC 
TTCGTCTGCA ACCAGGTTAT TGGAGGCGAT GTTTTCACTG CCTTGAACAA GGCCTGGTGT 
GTGCACCACT TTGCGTGCTC CGTGTGCGAC ACGAAGATGA CACAAAAATC GAAATTCTAC 
GAGTACGACG AGAAGCCGGT GTGCAAAAAG TGCTATGACC GATTTCCAAA CGAACTCAGA 
CGACGTCTGC GCACCGCCCA CGAGATGACA ATGAAAAAGA ACACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stck ( NM_079554.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524278.1 Drosophila melanogaster steamer duck (stck), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04247
Clone ID Related Accession (Same CDS sequence)	NM_001260058.2 , NM_079554.4
Accession Version	NM_079554.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	steamer duck, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079554.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTGG GTGCCATGCA TTGTACGCGA TGTGCCGACG GCTTCGAGCC GACTGAGAAG 
ATTGTTAACT CCAATGGAGA ACTCTGGCAC ACCCAGTGCT TCGTATGTGC CCAGTGCTTC 
CGACCCTTCC AGGATGGGAT ATTCTACGAG TTCGAGGGCC GTAAGTACTG TGAACGCGAC 
TTTCACGTGC TCTTCGCTCC CTGCTGCAAC AAGTGCGGCG AGTTCGTCAT CGGACGGGTC 
ATCAAGGCCA TGTCAGCTAG CTGGCATCCA CAGTGCTTCC GCTGCCAATT GTGCGCCAAG 
GAGCTGGCTG ATTGCGGATT CATCAAGAAC CAGAACCGCG CCTTGTGTCA CGAGTGCAAT 
GCCAAGGTGA AGGCGGAGAT TACCGGCCGC TATGTATGCC AAAAATGCCA CGGCCTCATC 
GACGAGGAGC CTTTGCGATT CCGGGGCGAA GTGTACCACG GCTATCACTT CAGTTGCACG 
GCCTGCGGCA CAGAGTTGGA TTCCACTGCT CGGGAGGTCA AGAGCCGGCC AGGATTGGCT 
GCTAACGATA TGAACGAACT GTATTGTCTG CGTTGTCACG ATAAAATGGG CATTCCCATA 
TGTGGTGCCT GTCGTCGTCC GATTGAGGAG AGGGTGGTAA CGGCGCTCGG AAAGCACTGG 
CATGTGGAAC ATTTCGTGTG CGCCAAGTGC GAGAAACCCT TCTTGGGTCA CCGACACTAC 
GAGAAGCGGG GATTGGCCTA CTGCGAGACG CACTATCATC AGCTCTTTGG CAACCTGTGC 
TTCGTCTGCA ACCAGGTTAT TGGAGGCGAT GTTTTCACTG CCTTGAACAA GGCCTGGTGT 
GTGCACCACT TTGCGTGCTC CGTGTGCGAC ACGAAGATGA CACAAAAATC GAAATTCTAC 
GAGTACGACG AGAAGCCGGT GTGCAAAAAG TGCTATGACC GATTTCCAAA CGAACTCAGA 
CGACGTCTGC GCACCGCCCA CGAGATGACA ATGAAAAAGA ACACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524278.1
CDS	135..1142
Translation	MSLGAMHCTRCADGFEPTEKIVNSNGELWHTQCFVCAQCFRPFQDGIFYEFEGRKYCERDFHVLFAPCCNKCGEFVIGRVIKAMSASWHPQCFRCQLCAKELADCGFIKNQNRALCHECNAKVKAEITGRYVCQKCHGLIDEEPLRFRGEVYHGYHFSCTACGTELDSTAREVKSRPGLAANDMNELYCLRCHDKMGIPICGACRRPIEERVVTALGKHWHVEHFVCAKCEKPFLGHRHYEKRGLAYCETHYHQLFGNLCFVCNQVIGGDVFTALNKAWCVHHFACSVCDTKMTQKSKFYEYDEKPVCKKCYDRFPNELRRRLRTAHEMTMKKNT

Target ORF information:

RefSeq Version	NM_079554.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster steamer duck (stck), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079554.4

ORF Insert Sequence:

ATGTCGCTGG GTGCCATGCA TTGTACGCGA TGTGCCGACG GCTTCGAGCC GACTGAGAAG 
ATTGTTAACT CCAATGGAGA ACTCTGGCAC ACCCAGTGCT TCGTATGTGC CCAGTGCTTC 
CGACCCTTCC AGGATGGGAT ATTCTACGAG TTCGAGGGCC GTAAGTACTG TGAACGCGAC 
TTTCACGTGC TCTTCGCTCC CTGCTGCAAC AAGTGCGGCG AGTTCGTCAT CGGACGGGTC 
ATCAAGGCCA TGTCAGCTAG CTGGCATCCA CAGTGCTTCC GCTGCCAATT GTGCGCCAAG 
GAGCTGGCTG ATTGCGGATT CATCAAGAAC CAGAACCGCG CCTTGTGTCA CGAGTGCAAT 
GCCAAGGTGA AGGCGGAGAT TACCGGCCGC TATGTATGCC AAAAATGCCA CGGCCTCATC 
GACGAGGAGC CTTTGCGATT CCGGGGCGAA GTGTACCACG GCTATCACTT CAGTTGCACG 
GCCTGCGGCA CAGAGTTGGA TTCCACTGCT CGGGAGGTCA AGAGCCGGCC AGGATTGGCT 
GCTAACGATA TGAACGAACT GTATTGTCTG CGTTGTCACG ATAAAATGGG CATTCCCATA 
TGTGGTGCCT GTCGTCGTCC GATTGAGGAG AGGGTGGTAA CGGCGCTCGG AAAGCACTGG 
CATGTGGAAC ATTTCGTGTG CGCCAAGTGC GAGAAACCCT TCTTGGGTCA CCGACACTAC 
GAGAAGCGGG GATTGGCCTA CTGCGAGACG CACTATCATC AGCTCTTTGG CAACCTGTGC 
TTCGTCTGCA ACCAGGTTAT TGGAGGCGAT GTTTTCACTG CCTTGAACAA GGCCTGGTGT 
GTGCACCACT TTGCGTGCTC CGTGTGCGAC ACGAAGATGA CACAAAAATC GAAATTCTAC 
GAGTACGACG AGAAGCCGGT GTGCAAAAAG TGCTATGACC GATTTCCAAA CGAACTCAGA 
CGACGTCTGC GCACCGCCCA CGAGATGACA ATGAAAAAGA ACACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stck ( NM_169220.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731241.1 Drosophila melanogaster steamer duck (stck), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08143
Clone ID Related Accession (Same CDS sequence)	NM_169220.3
Accession Version	NM_169220.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	steamer duck, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169220.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCCG TGCAAGTCCA GGCAGCGAAC ATGTCGCTGG GTGCCATGCA TTGTACGCGA 
TGTGCCGACG GCTTCGAGCC GACTGAGAAG ATTGTTAACT CCAATGGAGA ACTCTGGCAC 
ACCCAGTGCT TCGTATGTGC CCAGTGCTTC CGACCCTTCC AGGATGGGAT ATTCTACGAG 
TTCGAGGGCC GTAAGTACTG TGAACGCGAC TTTCACGTGC TCTTCGCTCC CTGCTGCAAC 
AAGTGCGGCG AGTTCGTCAT CGGACGGGTC ATCAAGGCCA TGTCAGCTAG CTGGCATCCA 
CAGTGCTTCC GCTGCCAATT GTGCGCCAAG GAGCTGGCTG ATTGCGGATT CATCAAGAAC 
CAGAACCGCG CCTTGTGTCA CGAGTGCAAT GCCAAGGTGA AGGCGGAGAT TACCGGCCGC 
TATGTATGCC AAAAATGCCA CGGCCTCATC GACGAGGAGC CTTTGCGATT CCGGGGCGAA 
GTGTACCACG GCTATCACTT CAGTTGCACG GCCTGCGGCA CAGAGTTGGA TTCCACTGCT 
CGGGAGGTCA AGAGCCGGCC AGGATTGGCT GCTAACGATA TGAACGAACT GTATTGTCTG 
CGTTGTCACG ATAAAATGGG CATTCCCATA TGTGGTGCCT GTCGTCGTCC GATTGAGGAG 
AGGGTGGTAA CGGCGCTCGG AAAGCACTGG CATGTGGAAC ATTTCGTGTG CGCCAAGTGC 
GAGAAACCCT TCTTGGGTCA CCGACACTAC GAGAAGCGGG GATTGGCCTA CTGCGAGACG 
CACTATCATC AGCTCTTTGG CAACCTGTGC TTCGTCTGCA ACCAGGTTAT TGGAGGCGAT 
GTTTTCACTG CCTTGAACAA GGCCTGGTGT GTGCACCACT TTGCGTGCTC CGTGTGCGAC 
ACGAAGATGA CACAAAAATC GAAATTCTAC GAGTACGACG AGAAGCCGGT GTGCAAAAAG 
TGCTATGACC GATTTCCAAA CGAACTCAGA CGACGTCTGC GCACCGCCCA CGAGATGACA 
ATGAAAAAGA ACACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731241.1
CDS	113..1150
Translation	MPPVQVQAANMSLGAMHCTRCADGFEPTEKIVNSNGELWHTQCFVCAQCFRPFQDGIFYEFEGRKYCERDFHVLFAPCCNKCGEFVIGRVIKAMSASWHPQCFRCQLCAKELADCGFIKNQNRALCHECNAKVKAEITGRYVCQKCHGLIDEEPLRFRGEVYHGYHFSCTACGTELDSTAREVKSRPGLAANDMNELYCLRCHDKMGIPICGACRRPIEERVVTALGKHWHVEHFVCAKCEKPFLGHRHYEKRGLAYCETHYHQLFGNLCFVCNQVIGGDVFTALNKAWCVHHFACSVCDTKMTQKSKFYEYDEKPVCKKCYDRFPNELRRRLRTAHEMTMKKNT

Target ORF information:

RefSeq Version	NM_169220.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster steamer duck (stck), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169220.3

ORF Insert Sequence:

ATGCCGCCCG TGCAAGTCCA GGCAGCGAAC ATGTCGCTGG GTGCCATGCA TTGTACGCGA 
TGTGCCGACG GCTTCGAGCC GACTGAGAAG ATTGTTAACT CCAATGGAGA ACTCTGGCAC 
ACCCAGTGCT TCGTATGTGC CCAGTGCTTC CGACCCTTCC AGGATGGGAT ATTCTACGAG 
TTCGAGGGCC GTAAGTACTG TGAACGCGAC TTTCACGTGC TCTTCGCTCC CTGCTGCAAC 
AAGTGCGGCG AGTTCGTCAT CGGACGGGTC ATCAAGGCCA TGTCAGCTAG CTGGCATCCA 
CAGTGCTTCC GCTGCCAATT GTGCGCCAAG GAGCTGGCTG ATTGCGGATT CATCAAGAAC 
CAGAACCGCG CCTTGTGTCA CGAGTGCAAT GCCAAGGTGA AGGCGGAGAT TACCGGCCGC 
TATGTATGCC AAAAATGCCA CGGCCTCATC GACGAGGAGC CTTTGCGATT CCGGGGCGAA 
GTGTACCACG GCTATCACTT CAGTTGCACG GCCTGCGGCA CAGAGTTGGA TTCCACTGCT 
CGGGAGGTCA AGAGCCGGCC AGGATTGGCT GCTAACGATA TGAACGAACT GTATTGTCTG 
CGTTGTCACG ATAAAATGGG CATTCCCATA TGTGGTGCCT GTCGTCGTCC GATTGAGGAG 
AGGGTGGTAA CGGCGCTCGG AAAGCACTGG CATGTGGAAC ATTTCGTGTG CGCCAAGTGC 
GAGAAACCCT TCTTGGGTCA CCGACACTAC GAGAAGCGGG GATTGGCCTA CTGCGAGACG 
CACTATCATC AGCTCTTTGG CAACCTGTGC TTCGTCTGCA ACCAGGTTAT TGGAGGCGAT 
GTTTTCACTG CCTTGAACAA GGCCTGGTGT GTGCACCACT TTGCGTGCTC CGTGTGCGAC 
ACGAAGATGA CACAAAAATC GAAATTCTAC GAGTACGACG AGAAGCCGGT GTGCAAAAAG 
TGCTATGACC GATTTCCAAA CGAACTCAGA CGACGTCTGC GCACCGCCCA CGAGATGACA 
ATGAAAAAGA ACACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stck ( NM_169221.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731242.1 Drosophila melanogaster steamer duck (stck), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08144
Clone ID Related Accession (Same CDS sequence)	NM_169221.3
Accession Version	NM_169221.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	steamer duck, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169221.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTACT CTAAGGCTGA AGTAGTCCAG GCAGCGAACA TGTCGCTGGG TGCCATGCAT 
TGTACGCGAT GTGCCGACGG CTTCGAGCCG ACTGAGAAGA TTGTTAACTC CAATGGAGAA 
CTCTGGCACA CCCAGTGCTT CGTATGTGCC CAGTGCTTCC GACCCTTCCA GGATGGGATA 
TTCTACGAGT TCGAGGGCCG TAAGTACTGT GAACGCGACT TTCACGTGCT CTTCGCTCCC 
TGCTGCAACA AGTGCGGCGA GTTCGTCATC GGACGGGTCA TCAAGGCCAT GTCAGCTAGC 
TGGCATCCAC AGTGCTTCCG CTGCCAATTG TGCGCCAAGG AGCTGGCTGA TTGCGGATTC 
ATCAAGAACC AGAACCGCGC CTTGTGTCAC GAGTGCAATG CCAAGGTGAA GGCGGAGATT 
ACCGGCCGCT ATGTATGCCA AAAATGCCAC GGCCTCATCG ACGAGGAGCC TTTGCGATTC 
CGGGGCGAAG TGTACCACGG CTATCACTTC AGTTGCACGG CCTGCGGCAC AGAGTTGGAT 
TCCACTGCTC GGGAGGTCAA GAGCCGGCCA GGATTGGCTG CTAACGATAT GAACGAACTG 
TATTGTCTGC GTTGTCACGA TAAAATGGGC ATTCCCATAT GTGGTGCCTG TCGTCGTCCG 
ATTGAGGAGA GGGTGGTAAC GGCGCTCGGA AAGCACTGGC ATGTGGAACA TTTCGTGTGC 
GCCAAGTGCG AGAAACCCTT CTTGGGTCAC CGACACTACG AGAAGCGGGG ATTGGCCTAC 
TGCGAGACGC ACTATCATCA GCTCTTTGGC AACCTGTGCT TCGTCTGCAA CCAGGTTATT 
GGAGGCGATG TTTTCACTGC CTTGAACAAG GCCTGGTGTG TGCACCACTT TGCGTGCTCC 
GTGTGCGACA CGAAGATGAC ACAAAAATCG AAATTCTACG AGTACGACGA GAAGCCGGTG 
TGCAAAAAGT GCTATGACCG ATTTCCAAAC GAACTCAGAC GACGTCTGCG CACCGCCCAC 
GAGATGACAA TGAAAAAGAA CACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731242.1
CDS	55..1101
Translation	MMYSKAEVVQAANMSLGAMHCTRCADGFEPTEKIVNSNGELWHTQCFVCAQCFRPFQDGIFYEFEGRKYCERDFHVLFAPCCNKCGEFVIGRVIKAMSASWHPQCFRCQLCAKELADCGFIKNQNRALCHECNAKVKAEITGRYVCQKCHGLIDEEPLRFRGEVYHGYHFSCTACGTELDSTAREVKSRPGLAANDMNELYCLRCHDKMGIPICGACRRPIEERVVTALGKHWHVEHFVCAKCEKPFLGHRHYEKRGLAYCETHYHQLFGNLCFVCNQVIGGDVFTALNKAWCVHHFACSVCDTKMTQKSKFYEYDEKPVCKKCYDRFPNELRRRLRTAHEMTMKKNT

Target ORF information:

RefSeq Version	NM_169221.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster steamer duck (stck), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169221.3

ORF Insert Sequence:

ATGATGTACT CTAAGGCTGA AGTAGTCCAG GCAGCGAACA TGTCGCTGGG TGCCATGCAT 
TGTACGCGAT GTGCCGACGG CTTCGAGCCG ACTGAGAAGA TTGTTAACTC CAATGGAGAA 
CTCTGGCACA CCCAGTGCTT CGTATGTGCC CAGTGCTTCC GACCCTTCCA GGATGGGATA 
TTCTACGAGT TCGAGGGCCG TAAGTACTGT GAACGCGACT TTCACGTGCT CTTCGCTCCC 
TGCTGCAACA AGTGCGGCGA GTTCGTCATC GGACGGGTCA TCAAGGCCAT GTCAGCTAGC 
TGGCATCCAC AGTGCTTCCG CTGCCAATTG TGCGCCAAGG AGCTGGCTGA TTGCGGATTC 
ATCAAGAACC AGAACCGCGC CTTGTGTCAC GAGTGCAATG CCAAGGTGAA GGCGGAGATT 
ACCGGCCGCT ATGTATGCCA AAAATGCCAC GGCCTCATCG ACGAGGAGCC TTTGCGATTC 
CGGGGCGAAG TGTACCACGG CTATCACTTC AGTTGCACGG CCTGCGGCAC AGAGTTGGAT 
TCCACTGCTC GGGAGGTCAA GAGCCGGCCA GGATTGGCTG CTAACGATAT GAACGAACTG 
TATTGTCTGC GTTGTCACGA TAAAATGGGC ATTCCCATAT GTGGTGCCTG TCGTCGTCCG 
ATTGAGGAGA GGGTGGTAAC GGCGCTCGGA AAGCACTGGC ATGTGGAACA TTTCGTGTGC 
GCCAAGTGCG AGAAACCCTT CTTGGGTCAC CGACACTACG AGAAGCGGGG ATTGGCCTAC 
TGCGAGACGC ACTATCATCA GCTCTTTGGC AACCTGTGCT TCGTCTGCAA CCAGGTTATT 
GGAGGCGATG TTTTCACTGC CTTGAACAAG GCCTGGTGTG TGCACCACTT TGCGTGCTCC 
GTGTGCGACA CGAAGATGAC ACAAAAATCG AAATTCTACG AGTACGACGA GAAGCCGGTG 
TGCAAAAAGT GCTATGACCG ATTTCCAAAC GAACTCAGAC GACGTCTGCG CACCGCCCAC 
GAGATGACAA TGAAAAAGAA CACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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stck cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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