RpA-70 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RpA-70
Entrez Gene ID	40972
Full Name	Replication Protein A 70
Synonyms	CG9633,D-RPA70,D-SSB,DRP-A,DmRPA,Dmel\CG9633,RP-A,RPA,RPA 70,RPA 70 kDa,RPA1,RPA70,RpA1,RpA70,Rpa-70,Ssb-70,dRP-A,dmrpa1,i164
General protein information	Preferred Names Replication Protein A 70 Names CG9633 gene product from transcript CG9633-RA CG9633-PA RpA-70-PA drosophila replication protein A replication protein A 70 single stranded-binding protein 70
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84F6-84F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_079550.4	NP_524274.1	replication protein A 70

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RpA-70	NP_524274.1
Saccharomyces cerevisiae (baker's yeast)	RFA1	NP_009404.1
Kluyveromyces lactis	KLLA0A08844g	XP_451388.1
Eremothecium gossypii	AGOS_AFL008W	NP_985540.1
Arabidopsis thaliana (thale cress)	RPA70C	NP_199353.1
Mus musculus (house mouse)	Rpa1	NP_001157695.1
Rattus norvegicus (Norway rat)	Rpa1	NP_001041308.1
Danio rerio (zebrafish)	rpa1	NP_956105.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001421	XP_321709.4
Schizosaccharomyces pombe (fission yeast)	ssb1	NP_595092.1
Canis lupus familiaris (dog)	RPA1	XP_003639310.1
Oryza sativa (rice)	Os05g0111000	NP_001054445.1
Xenopus tropicalis (tropical clawed frog)	rpa1	NP_001015732.1
Homo sapiens (human)	RPA1	NP_002936.1
Pan troglodytes (chimpanzee)	RPA1	XP_511254.3
Bos taurus (cattle)	RPA1	NP_001068644.1
Gallus gallus (chicken)	RPA1	NP_001006221.1
Magnaporthe oryzae (rice blast fungus)	MGG_00685	XP_003718324.1
Neurospora crassa	NCU03606	XP_961333.1
Arabidopsis thaliana (thale cress)	AT4G19130	NP_567576.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

loss-of-function alleles indicate that RPA is required to prevent neuroepithelial cells from differentiating into medulla neuroblasts
Title: Replication protein a links cell cycle progression and the onset of neurogenesis in Drosophila optic lobe development.

Data show that the basic cleft of the RPA70 N-terminal OB-fold domain binds multiple checkpoint proteins, including RAD9, to promote ATR signaling.
Title: The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling.

The following RpA-70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RpA-70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08113	NM_079550.4 Latest version!	Drosophila melanogaster replication protein A 70 (RpA-70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RpA-70 ( NM_079550.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524274.1 Drosophila melanogaster replication protein A 70 (RpA-70), mRNA.

Price & Availability↑

CloneID	OFa08113
Clone ID Related Accession (Same CDS sequence)	NM_079550.4
Accession Version	NM_079550.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	replication protein A 70
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079550.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCCTGG CATCTTTGTC CACTGGTGTG ATTGCTCGCA TTATGCATGG CGAGGTTGTG 
GATGCCCCGG TTCTCCAGAT CCTGGCAATC AAGAAGATTA ACAGCGCAGC GGACTCAGAG 
CGCTATCGCA TCCTAATCTC CGACGGCAAG TACTTTAACA GCTATGCGAT GCTGGCCAGT 
CAGCTGAACG TGATGCAGCA CAATGGAGAA TTAGAGGAGT TCACCATTGT CCAGCTAGAT 
AAATATGTGA CCTCACTGGT AGGCAAGGAC GGAGCCGGAA AACGCGTTCT CATCATTTCC 
GAACTAACTG TGGTGAATCC TGGCGCCGAG GTTAAGTCCA AAATTGGCGA ACCCGTTACG 
TATGAAAATG CAGCCAAGCA GGATCTGGCG CCTAAGCCAG CAGTAACATC TAATTCCAAG 
CCAATTGCCA AAAAGGAACC GAGCCACAAC AACAATAATA ACATAGTAAT GAATTCCTCT 
ATTAATAGTG GAATGACACA TCCCATTTCT AGCCTAAGTC CCTACCAAAA TAAGTGGGTG 
ATTAAGGCTC GCGTGACCTC AAAATCCGGC ATTCGCACCT GGAGCAATGC CCGCGGCGAG 
GGCAAGTTGT TCAGCATGGA TTTAATGGAC GAATCGGGCG AAATTCGGGC CACTGCCTTC 
AAGGAACAGT GCGATAAGTT CTACGACCTC ATTCAAGTGG ATAGCGTGTA CTATATATCA 
AAGTGTCAAT TGAAACCGGC AAACAAGCAG TACTCTTCGC TGAACAACGC CTACGAGATG 
ACTTTTTCCG GTGAAACAGT TGTCCAGCTT TGTGAGGATA CCGACGATGA TCCCATACCG 
GAAATTAAGT ACAACCTTGT TCCTATTTCC GATGTGTCGG GTATGGAAAA TAAAGCAGCG 
GTAGACACAA TAGGAATTTG CAAGGAGGTG GGCGAGTTGC AATCATTCGT AGCTCGCACC 
ACAAACAAGG AATTCAAGAA ACGGGACATA ACCCTAGTGG ACATGAGTAA CTCGGCCATC 
AGCTTGACCC TTTGGGGGGA TGATGCCGTT AACTTTGACG GTCATGTGCA GCCTGTGATC 
CTGGTCAAGG GAACACGCAT TAACGAGTTC AATGGCGGAA AGTCCTTGAG CCTCGGCGGT 
GGCTCAATCA TGAAGATTAA CCCCGATATA CCCGAGGCAC ATAAGCTGCG CGGCTGGTTC 
GACAATGGAG GTGGTGACAG TGTCGCCAAT ATGGTTTCGG CGCGAACGGG CGGAGGAAGC 
TTCTCTACTG AATGGATGAC GCTTAAGGAT GCGCGGGCTC GCAATCTCGG CAGCGGCGAC 
AAGCCTGATT ACTTCCAGTG CAAGGCAGTG GTGCACATTG TCAAACAGGA AAATGCTTTC 
TACCGAGCCT GCCCTCAGAG CGACTGCAAC AAGAAGGTTG TCGATGAGGG CAACGACCAG 
TTCCGCTGCG AGAAATGTAA CGCACTGTTT CCCAACTTTA AGTACCGACT GCTGATCAAC 
ATGAGCATCG GGGACTGGAC TTCCAACCGT TGGGTCAGCA GTTTTAACGA GGTCGGTGAA 
CAGCTTCTCG GGCACACTTC GCAGGAAGTG GGTGAGGCCC TTGAGAACGA CCCTGCCAAA 
GCCGAGCAGA TTTTCTCAGC ACTCAACTTC ACTTCACACA TCTTTAAGCT GCGCTGCAAG 
AACGAGGTTT ACGGGGACAT GACTCGCAAC AAGCTCACTG TGCAGTCTGT AGCCCCTATC 
AATCACAAGG AGTACAATAA GCACTTGCTC AAGGAGCTGC AGGAGCTAAC CGGCATTGGC 
TCATCAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524274.1
CDS	82..1893
Translation	MVLASLSTGVIARIMHGEVVDAPVLQILAIKKINSAADSERYRILISDGKYFNSYAMLASQLNVMQHNGELEEFTIVQLDKYVTSLVGKDGAGKRVLIISELTVVNPGAEVKSKIGEPVTYENAAKQDLAPKPAVTSNSKPIAKKEPSHNNNNNIVMNSSINSGMTHPISSLSPYQNKWVIKARVTSKSGIRTWSNARGEGKLFSMDLMDESGEIRATAFKEQCDKFYDLIQVDSVYYISKCQLKPANKQYSSLNNAYEMTFSGETVVQLCEDTDDDPIPEIKYNLVPISDVSGMENKAAVDTIGICKEVGELQSFVARTTNKEFKKRDITLVDMSNSAISLTLWGDDAVNFDGHVQPVILVKGTRINEFNGGKSLSLGGGSIMKINPDIPEAHKLRGWFDNGGGDSVANMVSARTGGGSFSTEWMTLKDARARNLGSGDKPDYFQCKAVVHIVKQENAFYRACPQSDCNKKVVDEGNDQFRCEKCNALFPNFKYRLLINMSIGDWTSNRWVSSFNEVGEQLLGHTSQEVGEALENDPAKAEQIFSALNFTSHIFKLRCKNEVYGDMTRNKLTVQSVAPINHKEYNKHLLKELQELTGIGSSN

Target ORF information:

RefSeq Version	NM_079550.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster replication protein A 70 (RpA-70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079550.4

ORF Insert Sequence:

ATGGTCCTGG CATCTTTGTC CACTGGTGTG ATTGCTCGCA TTATGCATGG CGAGGTTGTG 
GATGCCCCGG TTCTCCAGAT CCTGGCAATC AAGAAGATTA ACAGCGCAGC GGACTCAGAG 
CGCTATCGCA TCCTAATCTC CGACGGCAAG TACTTTAACA GCTATGCGAT GCTGGCCAGT 
CAGCTGAACG TGATGCAGCA CAATGGAGAA TTAGAGGAGT TCACCATTGT CCAGCTAGAT 
AAATATGTGA CCTCACTGGT AGGCAAGGAC GGAGCCGGAA AACGCGTTCT CATCATTTCC 
GAACTAACTG TGGTGAATCC TGGCGCCGAG GTTAAGTCCA AAATTGGCGA ACCCGTTACG 
TATGAAAATG CAGCCAAGCA GGATCTGGCG CCTAAGCCAG CAGTAACATC TAATTCCAAG 
CCAATTGCCA AAAAGGAACC GAGCCACAAC AACAATAATA ACATAGTAAT GAATTCCTCT 
ATTAATAGTG GAATGACACA TCCCATTTCT AGCCTAAGTC CCTACCAAAA TAAGTGGGTG 
ATTAAGGCTC GCGTGACCTC AAAATCCGGC ATTCGCACCT GGAGCAATGC CCGCGGCGAG 
GGCAAGTTGT TCAGCATGGA TTTAATGGAC GAATCGGGCG AAATTCGGGC CACTGCCTTC 
AAGGAACAGT GCGATAAGTT CTACGACCTC ATTCAAGTGG ATAGCGTGTA CTATATATCA 
AAGTGTCAAT TGAAACCGGC AAACAAGCAG TACTCTTCGC TGAACAACGC CTACGAGATG 
ACTTTTTCCG GTGAAACAGT TGTCCAGCTT TGTGAGGATA CCGACGATGA TCCCATACCG 
GAAATTAAGT ACAACCTTGT TCCTATTTCC GATGTGTCGG GTATGGAAAA TAAAGCAGCG 
GTAGACACAA TAGGAATTTG CAAGGAGGTG GGCGAGTTGC AATCATTCGT AGCTCGCACC 
ACAAACAAGG AATTCAAGAA ACGGGACATA ACCCTAGTGG ACATGAGTAA CTCGGCCATC 
AGCTTGACCC TTTGGGGGGA TGATGCCGTT AACTTTGACG GTCATGTGCA GCCTGTGATC 
CTGGTCAAGG GAACACGCAT TAACGAGTTC AATGGCGGAA AGTCCTTGAG CCTCGGCGGT 
GGCTCAATCA TGAAGATTAA CCCCGATATA CCCGAGGCAC ATAAGCTGCG CGGCTGGTTC 
GACAATGGAG GTGGTGACAG TGTCGCCAAT ATGGTTTCGG CGCGAACGGG CGGAGGAAGC 
TTCTCTACTG AATGGATGAC GCTTAAGGAT GCGCGGGCTC GCAATCTCGG CAGCGGCGAC 
AAGCCTGATT ACTTCCAGTG CAAGGCAGTG GTGCACATTG TCAAACAGGA AAATGCTTTC 
TACCGAGCCT GCCCTCAGAG CGACTGCAAC AAGAAGGTTG TCGATGAGGG CAACGACCAG 
TTCCGCTGCG AGAAATGTAA CGCACTGTTT CCCAACTTTA AGTACCGACT GCTGATCAAC 
ATGAGCATCG GGGACTGGAC TTCCAACCGT TGGGTCAGCA GTTTTAACGA GGTCGGTGAA 
CAGCTTCTCG GGCACACTTC GCAGGAAGTG GGTGAGGCCC TTGAGAACGA CCCTGCCAAA 
GCCGAGCAGA TTTTCTCAGC ACTCAACTTC ACTTCACACA TCTTTAAGCT GCGCTGCAAG 
AACGAGGTTT ACGGGGACAT GACTCGCAAC AAGCTCACTG TGCAGTCTGT AGCCCCTATC 
AATCACAAGG AGTACAATAA GCACTTGCTC AAGGAGCTGC AGGAGCTAAC CGGCATTGGC 
TCATCAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RpA-70 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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