Ada2b cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ada2b
Entrez Gene ID	40966
Full Name	transcriptional Adaptor 2b
Synonyms	ADA2b,Ada2B,Ada2S,BcDNA:LD24527,CG9638,Dmel\CG9638,ada2b,dADA2b,dAda2B,dAda2S,dAda2b
General protein information	Preferred Names transcriptional Adaptor 2b Names CG9638 gene product from transcript CG9638-RC Ada2b-PA Ada2b-PB Ada2b-PC CG9638-PA CG9638-PB CG9638-PC transcriptional adapter 2S transcriptional adaptor 2b
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84F5-84F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275438.1	NP_001262367.1	transcriptional adaptor 2b, isoform C
NM_141516.3	NP_649773.1	transcriptional adaptor 2b, isoform A
NM_001031980.2	NP_001027151.1	transcriptional adaptor 2b, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TADA2B	XP_001156769.1
Gallus gallus (chicken)	TADA2B	XP_426352.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003109	XP_312792.5
Canis lupus familiaris (dog)	TADA2B	XP_005618509.1
Danio rerio (zebrafish)	tada2b	NP_001019614.1
Homo sapiens (human)	TADA2B	NP_689506.2
Bos taurus (cattle)	TADA2B	NP_001193339.1
Mus musculus (house mouse)	Tada2b	NP_001163925.1
Rattus norvegicus (Norway rat)	Tada2b	NP_001163926.1
Xenopus tropicalis (tropical clawed frog)	tada2b	XP_002938304.1
Drosophila melanogaster (fruit fly)	Ada2b	NP_001027151.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

different isoforms of dADA2b contribute to the functional variations of dSAGA multiprotein HAT complexes
Title: Functional characterization and gene expression profiling of Drosophila melanogaster short dADA2b isoform-containing dSAGA complexes.

ADA2 C-terminal regions play an important role in the specific incorporation of ADA2 into SAGA- or ATAC-type complexes, which in turn determines H3- or H4-specific histone targeting.
Title: The C-terminal domains of ADA2 proteins determine selective incorporation into GCN5-containing complexes that target histone H3 or H4 for acetylation.

The ADA2b gene, functionally distinct homolog of ADA2a, is essential for the Drosophila development.
Title: The homologous Drosophila transcriptional adaptors ADA2a and ADA2b are both required for normal development but have different functions.

Ada2b is required for viability in Drosophila, and its deletion causes a reduction in histone H3 acetylation.
Title: Drosophila Ada2b is required for viability and normal histone H3 acetylation.

in vivo the two dADA2 proteins showed different localizations on polytene X chromosomes. These results, taken together, suggest that the two Drosophila ADA2 homologues are present in distinct GCN5-containing HAT complexes
Title: Two different Drosophila ADA2 homologues are present in distinct GCN5 histone acetyltransferase-containing complexes.

The following Ada2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ada2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04241	NM_141516.3 Latest version!	Drosophila melanogaster transcriptional adaptor 2b, transcript variant A (Ada2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04241	NM_001275438.1 Latest version!	Drosophila melanogaster transcriptional adaptor 2b, transcript variant C (Ada2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08107	NM_001031980.2 Latest version!	Drosophila melanogaster transcriptional adaptor 2b, transcript variant B (Ada2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ada2b ( NM_001275438.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262367.1 Drosophila melanogaster transcriptional adaptor 2b, transcript variant C (Ada2b), mRNA.

Price & Availability↑

CloneID	OFa04241
Clone ID Related Accession (Same CDS sequence)	NM_001275438.1 , NM_141516.3
Accession Version	NM_001275438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transcriptional adaptor 2b, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACAA TCGCGGATTT ATTCACAAAG TATAATTGCA CAAATTGCCA GGATGATATT 
CAGGGCATTC GAGTGCACTG CGCGGAGTGC GAAAACTTCG ATTTGTGTCT GCAATGCTTT 
GCCGCAGGTG CGGAGATCGG CGCCCATCAA AACAACCACT CCTACCAGTT CATGGACACC 
GGCACTTCCA TATTGAGTGT ATTTCGTGGA AAGGGGGCGT GGACGGCACG AGAGGAAATC 
CGCCTGTTGG ATGCAATCGA GCAGTACGGC TTTGGAAACT GGGAGGACAT TAGCAAGCAC 
ATTGAGACGA AGTCAGCGGA GGATGCCAAA GAGGAGTACG TGAATAAGTT TGTAAATGGC 
ACGATTGGAA GGGCCACTTG GACGCCGGCC CAGAGCCAGA GACCCCGCCT TATCGATCAC 
ACTGGAGACG ACGACGCTGG TCCTCTGGGC ACCAATGCTC TCTCTACCTT GCCTCCACTG 
GAAATCAACT CCGATGAGGC CATGCAGCTG GGCTACATGC CCAACAGGGA CAGCTTTGAG 
CGGGAGTACG ATCCCACGGC AGAGCAACTG ATCTCGAACA TCTCATTAAG CTCCGAGGAT 
ACGGAGGTGG ATGTGATGCT TAAGCTGGCC CACGTTGACA TATACACCCG CCGACTCAGA 
GAGCGTGCTC GCAGGAAACG GATGGTCAGG GACTATCAAT TAGTATCAAA TTTCTTCCGA 
AACAGAAATT ACGCTCAGCA ACAAGGACTC ACCAAGGAGC AGCGCGAGTT TCGGGACAGA 
TTTCGGGTCT ACGCCCAGTT CTACACGTGT AACGAGTATG AGCGGCTATT GGGATCACTG 
GAGCGCGAAA AGGAACTGCG CATTCGACAG TCAGAGCTGT ACCGATACCG GTATAATGGC 
CTGACCAAAA TCGCGGAATG CACACATTTC GAGCAGCACG CCGCAACAGC GACGCACCGC 
TCTACAGGAC CATATGGCCA TGGCAAGACC CTCCACTGCA TAATAAACGC GCCAAACAAC 
GAGCGTTCGA ATGGTTCTAT AAGACAATGT TTAACCACAG GACCACACGC ATACCTCCAA 
TGGAAGTCAT CGGCCGCCAA GCTCTTCCTT GCACTCCCCA CAACCGAATC TCAGAAAGGT 
CGAAATGTCA AGCGGCGGCG AGGCAAGTTC AAATTCAATC GCACCAAGAA ACACGCTCCA 
CATCGCCGAC CCGACCTGCT CCGGCGCATT ATTGCCCAGC AAAAACTACT TGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262367.1
CDS	93..1349
Translation	MTTIADLFTKYNCTNCQDDIQGIRVHCAECENFDLCLQCFAAGAEIGAHQNNHSYQFMDTGTSILSVFRGKGAWTAREEIRLLDAIEQYGFGNWEDISKHIETKSAEDAKEEYVNKFVNGTIGRATWTPAQSQRPRLIDHTGDDDAGPLGTNALSTLPPLEINSDEAMQLGYMPNRDSFEREYDPTAEQLISNISLSSEDTEVDVMLKLAHVDIYTRRLRERARRKRMVRDYQLVSNFFRNRNYAQQQGLTKEQREFRDRFRVYAQFYTCNEYERLLGSLEREKELRIRQSELYRYRYNGLTKIAECTHFEQHAATATHRSTGPYGHGKTLHCIINAPNNERSNGSIRQCLTTGPHAYLQWKSSAAKLFLALPTTESQKGRNVKRRRGKFKFNRTKKHAPHRRPDLLRRIIAQQKLLG

Target ORF information:

RefSeq Version	NM_001275438.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transcriptional adaptor 2b, transcript variant C (Ada2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275438.1

ORF Insert Sequence:

ATGACCACAA TCGCGGATTT ATTCACAAAG TATAATTGCA CAAATTGCCA GGATGATATT 
CAGGGCATTC GAGTGCACTG CGCGGAGTGC GAAAACTTCG ATTTGTGTCT GCAATGCTTT 
GCCGCAGGTG CGGAGATCGG CGCCCATCAA AACAACCACT CCTACCAGTT CATGGACACC 
GGCACTTCCA TATTGAGTGT ATTTCGTGGA AAGGGGGCGT GGACGGCACG AGAGGAAATC 
CGCCTGTTGG ATGCAATCGA GCAGTACGGC TTTGGAAACT GGGAGGACAT TAGCAAGCAC 
ATTGAGACGA AGTCAGCGGA GGATGCCAAA GAGGAGTACG TGAATAAGTT TGTAAATGGC 
ACGATTGGAA GGGCCACTTG GACGCCGGCC CAGAGCCAGA GACCCCGCCT TATCGATCAC 
ACTGGAGACG ACGACGCTGG TCCTCTGGGC ACCAATGCTC TCTCTACCTT GCCTCCACTG 
GAAATCAACT CCGATGAGGC CATGCAGCTG GGCTACATGC CCAACAGGGA CAGCTTTGAG 
CGGGAGTACG ATCCCACGGC AGAGCAACTG ATCTCGAACA TCTCATTAAG CTCCGAGGAT 
ACGGAGGTGG ATGTGATGCT TAAGCTGGCC CACGTTGACA TATACACCCG CCGACTCAGA 
GAGCGTGCTC GCAGGAAACG GATGGTCAGG GACTATCAAT TAGTATCAAA TTTCTTCCGA 
AACAGAAATT ACGCTCAGCA ACAAGGACTC ACCAAGGAGC AGCGCGAGTT TCGGGACAGA 
TTTCGGGTCT ACGCCCAGTT CTACACGTGT AACGAGTATG AGCGGCTATT GGGATCACTG 
GAGCGCGAAA AGGAACTGCG CATTCGACAG TCAGAGCTGT ACCGATACCG GTATAATGGC 
CTGACCAAAA TCGCGGAATG CACACATTTC GAGCAGCACG CCGCAACAGC GACGCACCGC 
TCTACAGGAC CATATGGCCA TGGCAAGACC CTCCACTGCA TAATAAACGC GCCAAACAAC 
GAGCGTTCGA ATGGTTCTAT AAGACAATGT TTAACCACAG GACCACACGC ATACCTCCAA 
TGGAAGTCAT CGGCCGCCAA GCTCTTCCTT GCACTCCCCA CAACCGAATC TCAGAAAGGT 
CGAAATGTCA AGCGGCGGCG AGGCAAGTTC AAATTCAATC GCACCAAGAA ACACGCTCCA 
CATCGCCGAC CCGACCTGCT CCGGCGCATT ATTGCCCAGC AAAAACTACT TGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ada2b ( NM_141516.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649773.1 Drosophila melanogaster transcriptional adaptor 2b, transcript variant A (Ada2b), mRNA.

Price & Availability↑

CloneID	OFa04241
Clone ID Related Accession (Same CDS sequence)	NM_001275438.1 , NM_141516.3
Accession Version	NM_141516.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transcriptional adaptor 2b, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141516.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACAA TCGCGGATTT ATTCACAAAG TATAATTGCA CAAATTGCCA GGATGATATT 
CAGGGCATTC GAGTGCACTG CGCGGAGTGC GAAAACTTCG ATTTGTGTCT GCAATGCTTT 
GCCGCAGGTG CGGAGATCGG CGCCCATCAA AACAACCACT CCTACCAGTT CATGGACACC 
GGCACTTCCA TATTGAGTGT ATTTCGTGGA AAGGGGGCGT GGACGGCACG AGAGGAAATC 
CGCCTGTTGG ATGCAATCGA GCAGTACGGC TTTGGAAACT GGGAGGACAT TAGCAAGCAC 
ATTGAGACGA AGTCAGCGGA GGATGCCAAA GAGGAGTACG TGAATAAGTT TGTAAATGGC 
ACGATTGGAA GGGCCACTTG GACGCCGGCC CAGAGCCAGA GACCCCGCCT TATCGATCAC 
ACTGGAGACG ACGACGCTGG TCCTCTGGGC ACCAATGCTC TCTCTACCTT GCCTCCACTG 
GAAATCAACT CCGATGAGGC CATGCAGCTG GGCTACATGC CCAACAGGGA CAGCTTTGAG 
CGGGAGTACG ATCCCACGGC AGAGCAACTG ATCTCGAACA TCTCATTAAG CTCCGAGGAT 
ACGGAGGTGG ATGTGATGCT TAAGCTGGCC CACGTTGACA TATACACCCG CCGACTCAGA 
GAGCGTGCTC GCAGGAAACG GATGGTCAGG GACTATCAAT TAGTATCAAA TTTCTTCCGA 
AACAGAAATT ACGCTCAGCA ACAAGGACTC ACCAAGGAGC AGCGCGAGTT TCGGGACAGA 
TTTCGGGTCT ACGCCCAGTT CTACACGTGT AACGAGTATG AGCGGCTATT GGGATCACTG 
GAGCGCGAAA AGGAACTGCG CATTCGACAG TCAGAGCTGT ACCGATACCG GTATAATGGC 
CTGACCAAAA TCGCGGAATG CACACATTTC GAGCAGCACG CCGCAACAGC GACGCACCGC 
TCTACAGGAC CATATGGCCA TGGCAAGACC CTCCACTGCA TAATAAACGC GCCAAACAAC 
GAGCGTTCGA ATGGTTCTAT AAGACAATGT TTAACCACAG GACCACACGC ATACCTCCAA 
TGGAAGTCAT CGGCCGCCAA GCTCTTCCTT GCACTCCCCA CAACCGAATC TCAGAAAGGT 
CGAAATGTCA AGCGGCGGCG AGGCAAGTTC AAATTCAATC GCACCAAGAA ACACGCTCCA 
CATCGCCGAC CCGACCTGCT CCGGCGCATT ATTGCCCAGC AAAAACTACT TGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649773.1
CDS	93..1349
Translation	MTTIADLFTKYNCTNCQDDIQGIRVHCAECENFDLCLQCFAAGAEIGAHQNNHSYQFMDTGTSILSVFRGKGAWTAREEIRLLDAIEQYGFGNWEDISKHIETKSAEDAKEEYVNKFVNGTIGRATWTPAQSQRPRLIDHTGDDDAGPLGTNALSTLPPLEINSDEAMQLGYMPNRDSFEREYDPTAEQLISNISLSSEDTEVDVMLKLAHVDIYTRRLRERARRKRMVRDYQLVSNFFRNRNYAQQQGLTKEQREFRDRFRVYAQFYTCNEYERLLGSLEREKELRIRQSELYRYRYNGLTKIAECTHFEQHAATATHRSTGPYGHGKTLHCIINAPNNERSNGSIRQCLTTGPHAYLQWKSSAAKLFLALPTTESQKGRNVKRRRGKFKFNRTKKHAPHRRPDLLRRIIAQQKLLG

Target ORF information:

RefSeq Version	NM_141516.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transcriptional adaptor 2b, transcript variant A (Ada2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141516.3

ORF Insert Sequence:

ATGACCACAA TCGCGGATTT ATTCACAAAG TATAATTGCA CAAATTGCCA GGATGATATT 
CAGGGCATTC GAGTGCACTG CGCGGAGTGC GAAAACTTCG ATTTGTGTCT GCAATGCTTT 
GCCGCAGGTG CGGAGATCGG CGCCCATCAA AACAACCACT CCTACCAGTT CATGGACACC 
GGCACTTCCA TATTGAGTGT ATTTCGTGGA AAGGGGGCGT GGACGGCACG AGAGGAAATC 
CGCCTGTTGG ATGCAATCGA GCAGTACGGC TTTGGAAACT GGGAGGACAT TAGCAAGCAC 
ATTGAGACGA AGTCAGCGGA GGATGCCAAA GAGGAGTACG TGAATAAGTT TGTAAATGGC 
ACGATTGGAA GGGCCACTTG GACGCCGGCC CAGAGCCAGA GACCCCGCCT TATCGATCAC 
ACTGGAGACG ACGACGCTGG TCCTCTGGGC ACCAATGCTC TCTCTACCTT GCCTCCACTG 
GAAATCAACT CCGATGAGGC CATGCAGCTG GGCTACATGC CCAACAGGGA CAGCTTTGAG 
CGGGAGTACG ATCCCACGGC AGAGCAACTG ATCTCGAACA TCTCATTAAG CTCCGAGGAT 
ACGGAGGTGG ATGTGATGCT TAAGCTGGCC CACGTTGACA TATACACCCG CCGACTCAGA 
GAGCGTGCTC GCAGGAAACG GATGGTCAGG GACTATCAAT TAGTATCAAA TTTCTTCCGA 
AACAGAAATT ACGCTCAGCA ACAAGGACTC ACCAAGGAGC AGCGCGAGTT TCGGGACAGA 
TTTCGGGTCT ACGCCCAGTT CTACACGTGT AACGAGTATG AGCGGCTATT GGGATCACTG 
GAGCGCGAAA AGGAACTGCG CATTCGACAG TCAGAGCTGT ACCGATACCG GTATAATGGC 
CTGACCAAAA TCGCGGAATG CACACATTTC GAGCAGCACG CCGCAACAGC GACGCACCGC 
TCTACAGGAC CATATGGCCA TGGCAAGACC CTCCACTGCA TAATAAACGC GCCAAACAAC 
GAGCGTTCGA ATGGTTCTAT AAGACAATGT TTAACCACAG GACCACACGC ATACCTCCAA 
TGGAAGTCAT CGGCCGCCAA GCTCTTCCTT GCACTCCCCA CAACCGAATC TCAGAAAGGT 
CGAAATGTCA AGCGGCGGCG AGGCAAGTTC AAATTCAATC GCACCAAGAA ACACGCTCCA 
CATCGCCGAC CCGACCTGCT CCGGCGCATT ATTGCCCAGC AAAAACTACT TGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ada2b ( NM_001031980.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027151.1 Drosophila melanogaster transcriptional adaptor 2b, transcript variant B (Ada2b), mRNA.

Price & Availability↑

CloneID	OFa08107
Clone ID Related Accession (Same CDS sequence)	NM_001031980.2
Accession Version	NM_001031980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transcriptional adaptor 2b, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001031980.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACAA TCGCGGATTT ATTCACAAAG TATAATTGCA CAAATTGCCA GGATGATATT 
CAGGGCATTC GAGTGCACTG CGCGGAGTGC GAAAACTTCG ATTTGTGTCT GCAATGCTTT 
GCCGCAGGTG CGGAGATCGG CGCCCATCAA AACAACCACT CCTACCAGTT CATGGACACC 
GGCACTTCCA TATTGAGTGT ATTTCGTGGA AAGGGGGCGT GGACGGCACG AGAGGAAATC 
CGCCTGTTGG ATGCAATCGA GCAGTACGGC TTTGGAAACT GGGAGGACAT TAGCAAGCAC 
ATTGAGACGA AGTCAGCGGA GGATGCCAAA GAGGAGTACG TGAATAAGTT TGTAAATGGC 
ACGATTGGAA GGGCCACTTG GACGCCGGCC CAGAGCCAGA GACCCCGCCT TATCGATCAC 
ACTGGAGACG ACGACGCTGG TCCTCTGGGC ACCAATGCTC TCTCTACCTT GCCTCCACTG 
GAAATCAACT CCGATGAGGC CATGCAGCTG GGCTACATGC CCAACAGGGA CAGCTTTGAG 
CGGGAGTACG ATCCCACGGC AGAGCAACTG ATCTCGAACA TCTCATTAAG CTCCGAGGAT 
ACGGAGGTGG ATGTGATGCT TAAGCTGGCC CACGTTGACA TATACACCCG CCGACTCAGA 
GAGCGTGCTC GCAGGAAACG GATGGTCAGG GACTATCAAT TAGTATCAAA TTTCTTCCGA 
AACAGAAATT ACGCTCAGCA ACAAGGACTC ACCAAGGAGC AGCGCGAGTT TCGGGACAGA 
TTTCGGGTCT ACGCCCAGTT CTACACGTGT AACGAGTATG AGCGGCTATT GGGATCACTG 
GAGCGCGAAA AGGAACTGCG CATTCGACAG TCAGAGCTGT ACCGATACCG GTATAATGGC 
CTGACCAAAA TCGCGGAATG CACACATTTC GAGCAGCACG CCGCAACAGC GACGCACCGC 
TCTACAGGAC CATATGGCCA TGGCAAGACC GACCACACGC ATACCTCCAA TGGAAGTCAT 
CGGCCGCCAA GCTCTTCCTT GCACTCCCCA CAACCGAATC TCAGAAAGGT CGAAATGTCA 
AGCGGCGGCG AGGCAAGTTC AAATTCAATC GCACCAAGAA ACACGCTCCA CATCGCCGAC 
CCGACCTGCT CCGGCGCATT ATTGCCCAGC AAAAACTACT TGGATAGCTG TCGGGGATCG 
TCGGCAGCTA CGATGCTGCA GACGACGGGA ATGGTAATGG GGGTAACAGT GGACTCGGGA 
GCGACGACGG GAGTGACTTC CACGGCTACG ACGATGGCGA ATCTGCCAAC GAACTCGGCG 
AAGGGAAGCC AACAACATCT GCAGCCGCTG CAGCAGCATC CTCAGCTCCT GCAAAGCGGC 
AACCAGCACA AAATGCAAAA CGAGGCCGCC GGCGGAGGCA GTGATCAGGT GCCCAGCATG 
AGCCTGAAAT TGCGCACCCA GCTGGAGGAA CTAAAACATC TGCCACAGCC GCCGGGCAGT 
GAACTGCTCA GTCACAACGA GCTGGATCTG TGCAAGAAGC ATAATATTAC GCCCACCACC 
TACCTCTCGG TTAAGACTGT CTGCTTGAGC GGAGCACCAT CGTTGGGTAG TCCTATGGAG 
ACTTCGCTGC GCAAGTTCTT TATCAAGTGC GGCTGGCTGA GCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027151.1
CDS	93..1760
Translation	MTTIADLFTKYNCTNCQDDIQGIRVHCAECENFDLCLQCFAAGAEIGAHQNNHSYQFMDTGTSILSVFRGKGAWTAREEIRLLDAIEQYGFGNWEDISKHIETKSAEDAKEEYVNKFVNGTIGRATWTPAQSQRPRLIDHTGDDDAGPLGTNALSTLPPLEINSDEAMQLGYMPNRDSFEREYDPTAEQLISNISLSSEDTEVDVMLKLAHVDIYTRRLRERARRKRMVRDYQLVSNFFRNRNYAQQQGLTKEQREFRDRFRVYAQFYTCNEYERLLGSLEREKELRIRQSELYRYRYNGLTKIAECTHFEQHAATATHRSTGPYGHGKTDHTHTSNGSHRPPSSSLHSPQPNLRKVEMSSGGEASSNSIAPRNTLHIADPTCSGALLPSKNYLDSCRGSSAATMLQTTGMVMGVTVDSGATTGVTSTATTMANLPTNSAKGSQQHLQPLQQHPQLLQSGNQHKMQNEAAGGGSDQVPSMSLKLRTQLEELKHLPQPPGSELLSHNELDLCKKHNITPTTYLSVKTVCLSGAPSLGSPMETSLRKFFIKCGWLSH

Target ORF information:

RefSeq Version	NM_001031980.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transcriptional adaptor 2b, transcript variant B (Ada2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031980.2

ORF Insert Sequence:

ATGACCACAA TCGCGGATTT ATTCACAAAG TATAATTGCA CAAATTGCCA GGATGATATT 
CAGGGCATTC GAGTGCACTG CGCGGAGTGC GAAAACTTCG ATTTGTGTCT GCAATGCTTT 
GCCGCAGGTG CGGAGATCGG CGCCCATCAA AACAACCACT CCTACCAGTT CATGGACACC 
GGCACTTCCA TATTGAGTGT ATTTCGTGGA AAGGGGGCGT GGACGGCACG AGAGGAAATC 
CGCCTGTTGG ATGCAATCGA GCAGTACGGC TTTGGAAACT GGGAGGACAT TAGCAAGCAC 
ATTGAGACGA AGTCAGCGGA GGATGCCAAA GAGGAGTACG TGAATAAGTT TGTAAATGGC 
ACGATTGGAA GGGCCACTTG GACGCCGGCC CAGAGCCAGA GACCCCGCCT TATCGATCAC 
ACTGGAGACG ACGACGCTGG TCCTCTGGGC ACCAATGCTC TCTCTACCTT GCCTCCACTG 
GAAATCAACT CCGATGAGGC CATGCAGCTG GGCTACATGC CCAACAGGGA CAGCTTTGAG 
CGGGAGTACG ATCCCACGGC AGAGCAACTG ATCTCGAACA TCTCATTAAG CTCCGAGGAT 
ACGGAGGTGG ATGTGATGCT TAAGCTGGCC CACGTTGACA TATACACCCG CCGACTCAGA 
GAGCGTGCTC GCAGGAAACG GATGGTCAGG GACTATCAAT TAGTATCAAA TTTCTTCCGA 
AACAGAAATT ACGCTCAGCA ACAAGGACTC ACCAAGGAGC AGCGCGAGTT TCGGGACAGA 
TTTCGGGTCT ACGCCCAGTT CTACACGTGT AACGAGTATG AGCGGCTATT GGGATCACTG 
GAGCGCGAAA AGGAACTGCG CATTCGACAG TCAGAGCTGT ACCGATACCG GTATAATGGC 
CTGACCAAAA TCGCGGAATG CACACATTTC GAGCAGCACG CCGCAACAGC GACGCACCGC 
TCTACAGGAC CATATGGCCA TGGCAAGACC GACCACACGC ATACCTCCAA TGGAAGTCAT 
CGGCCGCCAA GCTCTTCCTT GCACTCCCCA CAACCGAATC TCAGAAAGGT CGAAATGTCA 
AGCGGCGGCG AGGCAAGTTC AAATTCAATC GCACCAAGAA ACACGCTCCA CATCGCCGAC 
CCGACCTGCT CCGGCGCATT ATTGCCCAGC AAAAACTACT TGGATAGCTG TCGGGGATCG 
TCGGCAGCTA CGATGCTGCA GACGACGGGA ATGGTAATGG GGGTAACAGT GGACTCGGGA 
GCGACGACGG GAGTGACTTC CACGGCTACG ACGATGGCGA ATCTGCCAAC GAACTCGGCG 
AAGGGAAGCC AACAACATCT GCAGCCGCTG CAGCAGCATC CTCAGCTCCT GCAAAGCGGC 
AACCAGCACA AAATGCAAAA CGAGGCCGCC GGCGGAGGCA GTGATCAGGT GCCCAGCATG 
AGCCTGAAAT TGCGCACCCA GCTGGAGGAA CTAAAACATC TGCCACAGCC GCCGGGCAGT 
GAACTGCTCA GTCACAACGA GCTGGATCTG TGCAAGAAGC ATAATATTAC GCCCACCACC 
TACCTCTCGG TTAAGACTGT CTGCTTGAGC GGAGCACCAT CGTTGGGTAG TCCTATGGAG 
ACTTCGCTGC GCAAGTTCTT TATCAAGTGC GGCTGGCTGA GCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ada2b cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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