Sp7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sp7
Entrez Gene ID	40918
Full Name	Serine protease 7
Synonyms	CG3066,Dmel\CG3066,MP2,PAE,PAE1,SP7,Sp 7,anon-Ryu,proPO-AE,sp7
General protein information	Preferred Names Serine protease 7 Names CG3066 gene product from transcript CG3066-RA CG3066-PA CG3066-PE CG3066-PF Sp7-PA Sp7-PE Sp7-PF melanization protease 2 prophenoloxidase-activating enzyme serine protease 7
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84D14-84E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_141477.2	NP_649734.2	serine protease 7, isoform A
NM_169192.3	NP_731174.1	serine protease 7, isoform E
NM_169193.3	NP_731175.2	serine protease 7, isoform F

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	CLIPB9	XP_003436374.1
Drosophila melanogaster (fruit fly)	Sp7	NP_649734.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sp7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sp7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07946	NM_141477.2 Latest version!	Drosophila melanogaster serine protease 7, transcript variant A (Sp7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07946	NM_169192.3 Latest version!	Drosophila melanogaster serine protease 7, transcript variant E (Sp7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07946	NM_169193.3 Latest version!	Drosophila melanogaster serine protease 7, transcript variant F (Sp7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sp7 ( NM_141477.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649734.2 Drosophila melanogaster serine protease 7, transcript variant A (Sp7), mRNA.

Price & Availability↑

CloneID	OFa07946
Clone ID Related Accession (Same CDS sequence)	NM_141477.2 , NM_169192.3 , NM_169193.3
Accession Version	NM_141477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serine protease 7, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Nov 6, 2002 this sequence version replaced NM_141477.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAATCAA CACGCAAAGT AGTTGGGATT TTTTTGGCCA CCTGCCTGCT GCCTTTCACC 
GTTCTACAAA ATGTTGCGGC ACAAGGAAGT TGTAGGAATC CCAACCAGAA ACAGGGACAA 
TGTTTGTCCA TATACGACTG TCAAAGTCTG CTTTCCGTGA TTCAGCAGTC TTATGTCAGC 
CCGGAGGACA GAACCTTTCT TCGAAACTCA CAGTGCCTCG ATGGAGTAGG TCGCCAGCCA 
TATGTTTGCT GTACAAGTGA TCGCAGCTTT GGATCCCAGG AGGCCACCTC CGCAGCCCCT 
CCTCCAACGA CTACCAGTTC AAGTTCACGA GGTCAAGACG GTCAAGCGGG TCTGGGCAAC 
CTGCTGCCCT CGCCACCCAA GTGCGGGCCC CACTCCTTCA GCAATAAGGT CTATAATGGC 
AACGACACTG CTATTGACGA GTTCAACTGG ATGGCACTGC TTGAGTACGT GGATAACAGG 
GGACGGCGGG AACTCAGTTG CGGTGGCAGT TTGATCAACA ATCGTTACGT TCTTACCGCC 
GCCCATTGCG TCATTGGTGC GGTGGAAACG GAAGTAGGTC ACCTAACTAC CGTTCGGTTG 
GGGGAATACG ATACCAGCAA GGACGTGGAC TGCATAGATG ACATTTGCAA CCAGCCCATC 
CTACAATTGG GCATCGAACA GGCCACTGTA CATCCCCAAT ATGATCCTGC CAACAAGAAC 
CGCATTCACG ACATTGCCTT ATTGCGATTA GATCGGCCCG TTGTGCTTAA TGAGTATATT 
CAGCCGGTTT GTTTGCCTTT GGTAAGCACA CGAATGGCAA TCAATACCGG GGAGCTCTTG 
GTGGTCAGCG GGTGGGGCCG AACCACAACA GCCCGCAAGA GCACCATAAA GCAGCGATTG 
GATCTGCCGG TGAATGACCA CGATTACTGC GCACGAAAGT TCGCCACGAG GAATATCCAC 
CTGATCAGTT CCCAGCTCTG TGTTGGCGGG GAATTCTACC GCGACAGCTG TGATGGCGAC 
TCCGGCGGAC CGCTGATGCG AAGGGGTTTT GACCAGGCTT GGTACCAGGA GGGCGTGGTC 
TCCTTTGGCA ACCGCTGCGG ACTAGAGGGT TGGCCAGGTG TCTACACTCG GGTAGCCGAC 
TACATGGACT GGATCGTGGA GACCATTCGT CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649734.2
CDS	589..1764
Translation	MKSTRKVVGIFLATCLLPFTVLQNVAAQGSCRNPNQKQGQCLSIYDCQSLLSVIQQSYVSPEDRTFLRNSQCLDGVGRQPYVCCTSDRSFGSQEATSAAPPPTTTSSSSRGQDGQAGLGNLLPSPPKCGPHSFSNKVYNGNDTAIDEFNWMALLEYVDNRGRRELSCGGSLINNRYVLTAAHCVIGAVETEVGHLTTVRLGEYDTSKDVDCIDDICNQPILQLGIEQATVHPQYDPANKNRIHDIALLRLDRPVVLNEYIQPVCLPLVSTRMAINTGELLVVSGWGRTTTARKSTIKQRLDLPVNDHDYCARKFATRNIHLISSQLCVGGEFYRDSCDGDSGGPLMRRGFDQAWYQEGVVSFGNRCGLEGWPGVYTRVADYMDWIVETIRP

Target ORF information:

RefSeq Version	NM_141477.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serine protease 7, transcript variant A (Sp7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141477.2

ORF Insert Sequence:

ATGAAATCAA CACGCAAAGT AGTTGGGATT TTTTTGGCCA CCTGCCTGCT GCCTTTCACC 
GTTCTACAAA ATGTTGCGGC ACAAGGAAGT TGTAGGAATC CCAACCAGAA ACAGGGACAA 
TGTTTGTCCA TATACGACTG TCAAAGTCTG CTTTCCGTGA TTCAGCAGTC TTATGTCAGC 
CCGGAGGACA GAACCTTTCT TCGAAACTCA CAGTGCCTCG ATGGAGTAGG TCGCCAGCCA 
TATGTTTGCT GTACAAGTGA TCGCAGCTTT GGATCCCAGG AGGCCACCTC CGCAGCCCCT 
CCTCCAACGA CTACCAGTTC AAGTTCACGA GGTCAAGACG GTCAAGCGGG TCTGGGCAAC 
CTGCTGCCCT CGCCACCCAA GTGCGGGCCC CACTCCTTCA GCAATAAGGT CTATAATGGC 
AACGACACTG CTATTGACGA GTTCAACTGG ATGGCACTGC TTGAGTACGT GGATAACAGG 
GGACGGCGGG AACTCAGTTG CGGTGGCAGT TTGATCAACA ATCGTTACGT TCTTACCGCC 
GCCCATTGCG TCATTGGTGC GGTGGAAACG GAAGTAGGTC ACCTAACTAC CGTTCGGTTG 
GGGGAATACG ATACCAGCAA GGACGTGGAC TGCATAGATG ACATTTGCAA CCAGCCCATC 
CTACAATTGG GCATCGAACA GGCCACTGTA CATCCCCAAT ATGATCCTGC CAACAAGAAC 
CGCATTCACG ACATTGCCTT ATTGCGATTA GATCGGCCCG TTGTGCTTAA TGAGTATATT 
CAGCCGGTTT GTTTGCCTTT GGTAAGCACA CGAATGGCAA TCAATACCGG GGAGCTCTTG 
GTGGTCAGCG GGTGGGGCCG AACCACAACA GCCCGCAAGA GCACCATAAA GCAGCGATTG 
GATCTGCCGG TGAATGACCA CGATTACTGC GCACGAAAGT TCGCCACGAG GAATATCCAC 
CTGATCAGTT CCCAGCTCTG TGTTGGCGGG GAATTCTACC GCGACAGCTG TGATGGCGAC 
TCCGGCGGAC CGCTGATGCG AAGGGGTTTT GACCAGGCTT GGTACCAGGA GGGCGTGGTC 
TCCTTTGGCA ACCGCTGCGG ACTAGAGGGT TGGCCAGGTG TCTACACTCG GGTAGCCGAC 
TACATGGACT GGATCGTGGA GACCATTCGT CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sp7 ( NM_169192.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731174.1 Drosophila melanogaster serine protease 7, transcript variant E (Sp7), mRNA.

Price & Availability↑

CloneID	OFa07946
Clone ID Related Accession (Same CDS sequence)	NM_141477.2 , NM_169192.3 , NM_169193.3
Accession Version	NM_169192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serine protease 7, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169192.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAATCAA CACGCAAAGT AGTTGGGATT TTTTTGGCCA CCTGCCTGCT GCCTTTCACC 
GTTCTACAAA ATGTTGCGGC ACAAGGAAGT TGTAGGAATC CCAACCAGAA ACAGGGACAA 
TGTTTGTCCA TATACGACTG TCAAAGTCTG CTTTCCGTGA TTCAGCAGTC TTATGTCAGC 
CCGGAGGACA GAACCTTTCT TCGAAACTCA CAGTGCCTCG ATGGAGTAGG TCGCCAGCCA 
TATGTTTGCT GTACAAGTGA TCGCAGCTTT GGATCCCAGG AGGCCACCTC CGCAGCCCCT 
CCTCCAACGA CTACCAGTTC AAGTTCACGA GGTCAAGACG GTCAAGCGGG TCTGGGCAAC 
CTGCTGCCCT CGCCACCCAA GTGCGGGCCC CACTCCTTCA GCAATAAGGT CTATAATGGC 
AACGACACTG CTATTGACGA GTTCAACTGG ATGGCACTGC TTGAGTACGT GGATAACAGG 
GGACGGCGGG AACTCAGTTG CGGTGGCAGT TTGATCAACA ATCGTTACGT TCTTACCGCC 
GCCCATTGCG TCATTGGTGC GGTGGAAACG GAAGTAGGTC ACCTAACTAC CGTTCGGTTG 
GGGGAATACG ATACCAGCAA GGACGTGGAC TGCATAGATG ACATTTGCAA CCAGCCCATC 
CTACAATTGG GCATCGAACA GGCCACTGTA CATCCCCAAT ATGATCCTGC CAACAAGAAC 
CGCATTCACG ACATTGCCTT ATTGCGATTA GATCGGCCCG TTGTGCTTAA TGAGTATATT 
CAGCCGGTTT GTTTGCCTTT GGTAAGCACA CGAATGGCAA TCAATACCGG GGAGCTCTTG 
GTGGTCAGCG GGTGGGGCCG AACCACAACA GCCCGCAAGA GCACCATAAA GCAGCGATTG 
GATCTGCCGG TGAATGACCA CGATTACTGC GCACGAAAGT TCGCCACGAG GAATATCCAC 
CTGATCAGTT CCCAGCTCTG TGTTGGCGGG GAATTCTACC GCGACAGCTG TGATGGCGAC 
TCCGGCGGAC CGCTGATGCG AAGGGGTTTT GACCAGGCTT GGTACCAGGA GGGCGTGGTC 
TCCTTTGGCA ACCGCTGCGG ACTAGAGGGT TGGCCAGGTG TCTACACTCG GGTAGCCGAC 
TACATGGACT GGATCGTGGA GACCATTCGT CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731174.1
CDS	156..1331
Translation	MKSTRKVVGIFLATCLLPFTVLQNVAAQGSCRNPNQKQGQCLSIYDCQSLLSVIQQSYVSPEDRTFLRNSQCLDGVGRQPYVCCTSDRSFGSQEATSAAPPPTTTSSSSRGQDGQAGLGNLLPSPPKCGPHSFSNKVYNGNDTAIDEFNWMALLEYVDNRGRRELSCGGSLINNRYVLTAAHCVIGAVETEVGHLTTVRLGEYDTSKDVDCIDDICNQPILQLGIEQATVHPQYDPANKNRIHDIALLRLDRPVVLNEYIQPVCLPLVSTRMAINTGELLVVSGWGRTTTARKSTIKQRLDLPVNDHDYCARKFATRNIHLISSQLCVGGEFYRDSCDGDSGGPLMRRGFDQAWYQEGVVSFGNRCGLEGWPGVYTRVADYMDWIVETIRP

Target ORF information:

RefSeq Version	NM_169192.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serine protease 7, transcript variant E (Sp7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169192.3

ORF Insert Sequence:

ATGAAATCAA CACGCAAAGT AGTTGGGATT TTTTTGGCCA CCTGCCTGCT GCCTTTCACC 
GTTCTACAAA ATGTTGCGGC ACAAGGAAGT TGTAGGAATC CCAACCAGAA ACAGGGACAA 
TGTTTGTCCA TATACGACTG TCAAAGTCTG CTTTCCGTGA TTCAGCAGTC TTATGTCAGC 
CCGGAGGACA GAACCTTTCT TCGAAACTCA CAGTGCCTCG ATGGAGTAGG TCGCCAGCCA 
TATGTTTGCT GTACAAGTGA TCGCAGCTTT GGATCCCAGG AGGCCACCTC CGCAGCCCCT 
CCTCCAACGA CTACCAGTTC AAGTTCACGA GGTCAAGACG GTCAAGCGGG TCTGGGCAAC 
CTGCTGCCCT CGCCACCCAA GTGCGGGCCC CACTCCTTCA GCAATAAGGT CTATAATGGC 
AACGACACTG CTATTGACGA GTTCAACTGG ATGGCACTGC TTGAGTACGT GGATAACAGG 
GGACGGCGGG AACTCAGTTG CGGTGGCAGT TTGATCAACA ATCGTTACGT TCTTACCGCC 
GCCCATTGCG TCATTGGTGC GGTGGAAACG GAAGTAGGTC ACCTAACTAC CGTTCGGTTG 
GGGGAATACG ATACCAGCAA GGACGTGGAC TGCATAGATG ACATTTGCAA CCAGCCCATC 
CTACAATTGG GCATCGAACA GGCCACTGTA CATCCCCAAT ATGATCCTGC CAACAAGAAC 
CGCATTCACG ACATTGCCTT ATTGCGATTA GATCGGCCCG TTGTGCTTAA TGAGTATATT 
CAGCCGGTTT GTTTGCCTTT GGTAAGCACA CGAATGGCAA TCAATACCGG GGAGCTCTTG 
GTGGTCAGCG GGTGGGGCCG AACCACAACA GCCCGCAAGA GCACCATAAA GCAGCGATTG 
GATCTGCCGG TGAATGACCA CGATTACTGC GCACGAAAGT TCGCCACGAG GAATATCCAC 
CTGATCAGTT CCCAGCTCTG TGTTGGCGGG GAATTCTACC GCGACAGCTG TGATGGCGAC 
TCCGGCGGAC CGCTGATGCG AAGGGGTTTT GACCAGGCTT GGTACCAGGA GGGCGTGGTC 
TCCTTTGGCA ACCGCTGCGG ACTAGAGGGT TGGCCAGGTG TCTACACTCG GGTAGCCGAC 
TACATGGACT GGATCGTGGA GACCATTCGT CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sp7 ( NM_169193.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731175.2 Drosophila melanogaster serine protease 7, transcript variant F (Sp7), mRNA.

Price & Availability↑

CloneID	OFa07946
Clone ID Related Accession (Same CDS sequence)	NM_141477.2 , NM_169192.3 , NM_169193.3
Accession Version	NM_169193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serine protease 7, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_169193.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAATCAA CACGCAAAGT AGTTGGGATT TTTTTGGCCA CCTGCCTGCT GCCTTTCACC 
GTTCTACAAA ATGTTGCGGC ACAAGGAAGT TGTAGGAATC CCAACCAGAA ACAGGGACAA 
TGTTTGTCCA TATACGACTG TCAAAGTCTG CTTTCCGTGA TTCAGCAGTC TTATGTCAGC 
CCGGAGGACA GAACCTTTCT TCGAAACTCA CAGTGCCTCG ATGGAGTAGG TCGCCAGCCA 
TATGTTTGCT GTACAAGTGA TCGCAGCTTT GGATCCCAGG AGGCCACCTC CGCAGCCCCT 
CCTCCAACGA CTACCAGTTC AAGTTCACGA GGTCAAGACG GTCAAGCGGG TCTGGGCAAC 
CTGCTGCCCT CGCCACCCAA GTGCGGGCCC CACTCCTTCA GCAATAAGGT CTATAATGGC 
AACGACACTG CTATTGACGA GTTCAACTGG ATGGCACTGC TTGAGTACGT GGATAACAGG 
GGACGGCGGG AACTCAGTTG CGGTGGCAGT TTGATCAACA ATCGTTACGT TCTTACCGCC 
GCCCATTGCG TCATTGGTGC GGTGGAAACG GAAGTAGGTC ACCTAACTAC CGTTCGGTTG 
GGGGAATACG ATACCAGCAA GGACGTGGAC TGCATAGATG ACATTTGCAA CCAGCCCATC 
CTACAATTGG GCATCGAACA GGCCACTGTA CATCCCCAAT ATGATCCTGC CAACAAGAAC 
CGCATTCACG ACATTGCCTT ATTGCGATTA GATCGGCCCG TTGTGCTTAA TGAGTATATT 
CAGCCGGTTT GTTTGCCTTT GGTAAGCACA CGAATGGCAA TCAATACCGG GGAGCTCTTG 
GTGGTCAGCG GGTGGGGCCG AACCACAACA GCCCGCAAGA GCACCATAAA GCAGCGATTG 
GATCTGCCGG TGAATGACCA CGATTACTGC GCACGAAAGT TCGCCACGAG GAATATCCAC 
CTGATCAGTT CCCAGCTCTG TGTTGGCGGG GAATTCTACC GCGACAGCTG TGATGGCGAC 
TCCGGCGGAC CGCTGATGCG AAGGGGTTTT GACCAGGCTT GGTACCAGGA GGGCGTGGTC 
TCCTTTGGCA ACCGCTGCGG ACTAGAGGGT TGGCCAGGTG TCTACACTCG GGTAGCCGAC 
TACATGGACT GGATCGTGGA GACCATTCGT CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731175.2
CDS	156..1331
Translation	MKSTRKVVGIFLATCLLPFTVLQNVAAQGSCRNPNQKQGQCLSIYDCQSLLSVIQQSYVSPEDRTFLRNSQCLDGVGRQPYVCCTSDRSFGSQEATSAAPPPTTTSSSSRGQDGQAGLGNLLPSPPKCGPHSFSNKVYNGNDTAIDEFNWMALLEYVDNRGRRELSCGGSLINNRYVLTAAHCVIGAVETEVGHLTTVRLGEYDTSKDVDCIDDICNQPILQLGIEQATVHPQYDPANKNRIHDIALLRLDRPVVLNEYIQPVCLPLVSTRMAINTGELLVVSGWGRTTTARKSTIKQRLDLPVNDHDYCARKFATRNIHLISSQLCVGGEFYRDSCDGDSGGPLMRRGFDQAWYQEGVVSFGNRCGLEGWPGVYTRVADYMDWIVETIRP

Target ORF information:

RefSeq Version	NM_169193.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serine protease 7, transcript variant F (Sp7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169193.3

ORF Insert Sequence:

ATGAAATCAA CACGCAAAGT AGTTGGGATT TTTTTGGCCA CCTGCCTGCT GCCTTTCACC 
GTTCTACAAA ATGTTGCGGC ACAAGGAAGT TGTAGGAATC CCAACCAGAA ACAGGGACAA 
TGTTTGTCCA TATACGACTG TCAAAGTCTG CTTTCCGTGA TTCAGCAGTC TTATGTCAGC 
CCGGAGGACA GAACCTTTCT TCGAAACTCA CAGTGCCTCG ATGGAGTAGG TCGCCAGCCA 
TATGTTTGCT GTACAAGTGA TCGCAGCTTT GGATCCCAGG AGGCCACCTC CGCAGCCCCT 
CCTCCAACGA CTACCAGTTC AAGTTCACGA GGTCAAGACG GTCAAGCGGG TCTGGGCAAC 
CTGCTGCCCT CGCCACCCAA GTGCGGGCCC CACTCCTTCA GCAATAAGGT CTATAATGGC 
AACGACACTG CTATTGACGA GTTCAACTGG ATGGCACTGC TTGAGTACGT GGATAACAGG 
GGACGGCGGG AACTCAGTTG CGGTGGCAGT TTGATCAACA ATCGTTACGT TCTTACCGCC 
GCCCATTGCG TCATTGGTGC GGTGGAAACG GAAGTAGGTC ACCTAACTAC CGTTCGGTTG 
GGGGAATACG ATACCAGCAA GGACGTGGAC TGCATAGATG ACATTTGCAA CCAGCCCATC 
CTACAATTGG GCATCGAACA GGCCACTGTA CATCCCCAAT ATGATCCTGC CAACAAGAAC 
CGCATTCACG ACATTGCCTT ATTGCGATTA GATCGGCCCG TTGTGCTTAA TGAGTATATT 
CAGCCGGTTT GTTTGCCTTT GGTAAGCACA CGAATGGCAA TCAATACCGG GGAGCTCTTG 
GTGGTCAGCG GGTGGGGCCG AACCACAACA GCCCGCAAGA GCACCATAAA GCAGCGATTG 
GATCTGCCGG TGAATGACCA CGATTACTGC GCACGAAAGT TCGCCACGAG GAATATCCAC 
CTGATCAGTT CCCAGCTCTG TGTTGGCGGG GAATTCTACC GCGACAGCTG TGATGGCGAC 
TCCGGCGGAC CGCTGATGCG AAGGGGTTTT GACCAGGCTT GGTACCAGGA GGGCGTGGTC 
TCCTTTGGCA ACCGCTGCGG ACTAGAGGGT TGGCCAGGTG TCTACACTCG GGTAGCCGAC 
TACATGGACT GGATCGTGGA GACCATTCGT CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sp7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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