CG1227 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG1227
Entrez Gene ID	40872
Full Name	CG1227 gene product from transcript CG1227-RA
Synonyms	Dmel\CG1227,MPSK/PSK,Psk
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84D3-84D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_141455.4	NP_649712.1	uncharacterized protein, isoform A
NM_001300278.1	NP_001287207.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	STK16	NP_001008910.1
Pan troglodytes (chimpanzee)	STK16	XP_001162451.1
Eremothecium gossypii	AGOS_AFL143C	NP_985407.1
Magnaporthe oryzae (rice blast fungus)	MGG_04463	XP_003710998.1
Arabidopsis thaliana (thale cress)	PK3	NP_196433.1
Canis lupus familiaris (dog)	STK16	XP_545656.1
Drosophila melanogaster (fruit fly)	CG1227	NP_649712.1
Saccharomyces cerevisiae (baker's yeast)	ENV7	NP_015088.1
Neurospora crassa	NCU07399	XP_958483.1
	STK16	XP_001101843.1
Bos taurus (cattle)	STK16	NP_001039720.1
Rattus norvegicus (Norway rat)	Stk16	NP_775165.1
Mus musculus (house mouse)	Stk16	NP_001264921.1
Gallus gallus (chicken)	STK16	XP_426593.1
Schizosaccharomyces pombe (fission yeast)	ppk13	NP_593555.1
Oryza sativa (rice)	Os09g0372800	NP_001063027.1
Xenopus tropicalis (tropical clawed frog)	stk16	NP_001004792.1
Danio rerio (zebrafish)	stk16	NP_001017630.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG1227 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG1227 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04181	NM_141455.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG1227), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa04181	NM_001300278.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG1227), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG1227 ( NM_141455.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649712.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG1227), mRNA.

Price & Availability↑

CloneID	OFa04181
Clone ID Related Accession (Same CDS sequence)	NM_141455.4 , NM_001300278.1
Accession Version	NM_141455.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141455.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAGTA TAGGATGGAC GCTGATCATG AAACGCGGCT GCCTCTTTAG TTGCCGCAAG 
GAGACCCTGA ACATAAACGG ATCTCGATAC ACAATCCGCG AAAGGCTGGC GACGGGGGGC 
TTCAGTCTAA TCGACCTTGG GGAAAACGCC TCCACGCGAC GTAGCTATGC GATAAAGCGC 
ATCACCTGCC ACAGCATCGA TGATCAGAAT ATAGCTCTGC GAGAAATCGA AAACTGTCGA 
AAAATTGACT CGGAAAACGT GATCCGGGTA GTGGATTACG AGCTGAAGGG CCAGGCGGAC 
ATAGTTATAA ACACAACGAG CACCTTGTTC ATTGTGCTTC CCTATTACAA GCACGGATCT 
CTTGCGGACC ACCTTCAGCT GCGATCGCGG AAACAGGACC ACATGCCCGA GGCCCAGATC 
CTGCAAATCT TTCTGGGCGT CTGCGAGGGA TTGAAGGCCA TCCACGAGGC AATGCCGGTG 
CCTCTGGCCC ACCGAGATCT AAAAACGGCC AACATCTGCC TGTCGGACTC TTTTGAGCCA 
ATTATTGTGG ATCTCGGGTC AATGACAGAA GCCCGACTGC AGATTGTGGG CCAGACGGAT 
GCTCAGCGCT TGCAGGACGA AGCCGAGGAG AGGAGCTCCA TTGTCTACCG GGCTCCAGAG 
TTATTTACCG TGAAAACATA TTGCACAATT GACGAGCGCA CTGACATATG GAGTCTTGGC 
TGTGTGCTCT ATGCAATGTG CTACTTTAAT TCACCCTACG ATCCCATCTA CGAGCGAGGT 
GACAGCGTGG CTTTGGCTGT GCTCAGTGGG AACATAAACA TTCCAGAGGA TTCAATTTAC 
ACAGAGGACA TGCACGAATT AATTAAGTAC ATGCTGCGAA CAGATCCAAT GGAACGGCCA 
TTCGTGTTTA GTGTTATCGA AAGGACCCAC GATCTGATAC AGAAACTGGA GGGTCGCTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649712.1
CDS	319..1281
Translation	MQSIGWTLIMKRGCLFSCRKETLNINGSRYTIRERLATGGFSLIDLGENASTRRSYAIKRITCHSIDDQNIALREIENCRKIDSENVIRVVDYELKGQADIVINTTSTLFIVLPYYKHGSLADHLQLRSRKQDHMPEAQILQIFLGVCEGLKAIHEAMPVPLAHRDLKTANICLSDSFEPIIVDLGSMTEARLQIVGQTDAQRLQDEAEERSSIVYRAPELFTVKTYCTIDERTDIWSLGCVLYAMCYFNSPYDPIYERGDSVALAVLSGNINIPEDSIYTEDMHELIKYMLRTDPMERPFVFSVIERTHDLIQKLEGRL

Target ORF information:

RefSeq Version	NM_141455.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG1227), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141455.4

ORF Insert Sequence:

ATGCAGAGTA TAGGATGGAC GCTGATCATG AAACGCGGCT GCCTCTTTAG TTGCCGCAAG 
GAGACCCTGA ACATAAACGG ATCTCGATAC ACAATCCGCG AAAGGCTGGC GACGGGGGGC 
TTCAGTCTAA TCGACCTTGG GGAAAACGCC TCCACGCGAC GTAGCTATGC GATAAAGCGC 
ATCACCTGCC ACAGCATCGA TGATCAGAAT ATAGCTCTGC GAGAAATCGA AAACTGTCGA 
AAAATTGACT CGGAAAACGT GATCCGGGTA GTGGATTACG AGCTGAAGGG CCAGGCGGAC 
ATAGTTATAA ACACAACGAG CACCTTGTTC ATTGTGCTTC CCTATTACAA GCACGGATCT 
CTTGCGGACC ACCTTCAGCT GCGATCGCGG AAACAGGACC ACATGCCCGA GGCCCAGATC 
CTGCAAATCT TTCTGGGCGT CTGCGAGGGA TTGAAGGCCA TCCACGAGGC AATGCCGGTG 
CCTCTGGCCC ACCGAGATCT AAAAACGGCC AACATCTGCC TGTCGGACTC TTTTGAGCCA 
ATTATTGTGG ATCTCGGGTC AATGACAGAA GCCCGACTGC AGATTGTGGG CCAGACGGAT 
GCTCAGCGCT TGCAGGACGA AGCCGAGGAG AGGAGCTCCA TTGTCTACCG GGCTCCAGAG 
TTATTTACCG TGAAAACATA TTGCACAATT GACGAGCGCA CTGACATATG GAGTCTTGGC 
TGTGTGCTCT ATGCAATGTG CTACTTTAAT TCACCCTACG ATCCCATCTA CGAGCGAGGT 
GACAGCGTGG CTTTGGCTGT GCTCAGTGGG AACATAAACA TTCCAGAGGA TTCAATTTAC 
ACAGAGGACA TGCACGAATT AATTAAGTAC ATGCTGCGAA CAGATCCAAT GGAACGGCCA 
TTCGTGTTTA GTGTTATCGA AAGGACCCAC GATCTGATAC AGAAACTGGA GGGTCGCTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG1227 ( NM_001300278.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287207.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG1227), mRNA.

Price & Availability↑

CloneID	OFa04181
Clone ID Related Accession (Same CDS sequence)	NM_141455.4 , NM_001300278.1
Accession Version	NM_001300278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAGTA TAGGATGGAC GCTGATCATG AAACGCGGCT GCCTCTTTAG TTGCCGCAAG 
GAGACCCTGA ACATAAACGG ATCTCGATAC ACAATCCGCG AAAGGCTGGC GACGGGGGGC 
TTCAGTCTAA TCGACCTTGG GGAAAACGCC TCCACGCGAC GTAGCTATGC GATAAAGCGC 
ATCACCTGCC ACAGCATCGA TGATCAGAAT ATAGCTCTGC GAGAAATCGA AAACTGTCGA 
AAAATTGACT CGGAAAACGT GATCCGGGTA GTGGATTACG AGCTGAAGGG CCAGGCGGAC 
ATAGTTATAA ACACAACGAG CACCTTGTTC ATTGTGCTTC CCTATTACAA GCACGGATCT 
CTTGCGGACC ACCTTCAGCT GCGATCGCGG AAACAGGACC ACATGCCCGA GGCCCAGATC 
CTGCAAATCT TTCTGGGCGT CTGCGAGGGA TTGAAGGCCA TCCACGAGGC AATGCCGGTG 
CCTCTGGCCC ACCGAGATCT AAAAACGGCC AACATCTGCC TGTCGGACTC TTTTGAGCCA 
ATTATTGTGG ATCTCGGGTC AATGACAGAA GCCCGACTGC AGATTGTGGG CCAGACGGAT 
GCTCAGCGCT TGCAGGACGA AGCCGAGGAG AGGAGCTCCA TTGTCTACCG GGCTCCAGAG 
TTATTTACCG TGAAAACATA TTGCACAATT GACGAGCGCA CTGACATATG GAGTCTTGGC 
TGTGTGCTCT ATGCAATGTG CTACTTTAAT TCACCCTACG ATCCCATCTA CGAGCGAGGT 
GACAGCGTGG CTTTGGCTGT GCTCAGTGGG AACATAAACA TTCCAGAGGA TTCAATTTAC 
ACAGAGGACA TGCACGAATT AATTAAGTAC ATGCTGCGAA CAGATCCAAT GGAACGGCCA 
TTCGTGTTTA GTGTTATCGA AAGGACCCAC GATCTGATAC AGAAACTGGA GGGTCGCTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287207.1
CDS	78..1040
Translation	MQSIGWTLIMKRGCLFSCRKETLNINGSRYTIRERLATGGFSLIDLGENASTRRSYAIKRITCHSIDDQNIALREIENCRKIDSENVIRVVDYELKGQADIVINTTSTLFIVLPYYKHGSLADHLQLRSRKQDHMPEAQILQIFLGVCEGLKAIHEAMPVPLAHRDLKTANICLSDSFEPIIVDLGSMTEARLQIVGQTDAQRLQDEAEERSSIVYRAPELFTVKTYCTIDERTDIWSLGCVLYAMCYFNSPYDPIYERGDSVALAVLSGNINIPEDSIYTEDMHELIKYMLRTDPMERPFVFSVIERTHDLIQKLEGRL

Target ORF information:

RefSeq Version	NM_001300278.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG1227), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300278.1

ORF Insert Sequence:

ATGCAGAGTA TAGGATGGAC GCTGATCATG AAACGCGGCT GCCTCTTTAG TTGCCGCAAG 
GAGACCCTGA ACATAAACGG ATCTCGATAC ACAATCCGCG AAAGGCTGGC GACGGGGGGC 
TTCAGTCTAA TCGACCTTGG GGAAAACGCC TCCACGCGAC GTAGCTATGC GATAAAGCGC 
ATCACCTGCC ACAGCATCGA TGATCAGAAT ATAGCTCTGC GAGAAATCGA AAACTGTCGA 
AAAATTGACT CGGAAAACGT GATCCGGGTA GTGGATTACG AGCTGAAGGG CCAGGCGGAC 
ATAGTTATAA ACACAACGAG CACCTTGTTC ATTGTGCTTC CCTATTACAA GCACGGATCT 
CTTGCGGACC ACCTTCAGCT GCGATCGCGG AAACAGGACC ACATGCCCGA GGCCCAGATC 
CTGCAAATCT TTCTGGGCGT CTGCGAGGGA TTGAAGGCCA TCCACGAGGC AATGCCGGTG 
CCTCTGGCCC ACCGAGATCT AAAAACGGCC AACATCTGCC TGTCGGACTC TTTTGAGCCA 
ATTATTGTGG ATCTCGGGTC AATGACAGAA GCCCGACTGC AGATTGTGGG CCAGACGGAT 
GCTCAGCGCT TGCAGGACGA AGCCGAGGAG AGGAGCTCCA TTGTCTACCG GGCTCCAGAG 
TTATTTACCG TGAAAACATA TTGCACAATT GACGAGCGCA CTGACATATG GAGTCTTGGC 
TGTGTGCTCT ATGCAATGTG CTACTTTAAT TCACCCTACG ATCCCATCTA CGAGCGAGGT 
GACAGCGTGG CTTTGGCTGT GCTCAGTGGG AACATAAACA TTCCAGAGGA TTCAATTTAC 
ACAGAGGACA TGCACGAATT AATTAAGTAC ATGCTGCGAA CAGATCCAAT GGAACGGCCA 
TTCGTGTTTA GTGTTATCGA AAGGACCCAC GATCTGATAC AGAAACTGGA GGGTCGCTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG1227 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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