Sas-4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sas-4
Entrez Gene ID	40859
Full Name	Spindle assembly abnormal 4
Synonyms	CG10061,CPAP,D-Sas4,D-sas4,DSAS-4,DSas-4,DSas4,Dmel\CG10061,Dsas-4,Dsas4,SAS-4,SAS4,Sas4,d-SAS-4,dsas-4,l(3)S2214,l(3)s2214,sas-4,sas4
General protein information	Preferred Names Spindle assembly abnormal 4 Names CG10061 gene product from transcript CG10061-RA CG10061-PA DSas-4 Sas-4-PA drosophila Sas-4 lethal (3) s2214 spindle assembly abnormal 4 spindle assembly abnormal 4 ortholog
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84C6-84C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_141444.2	NP_649701.2	spindle assembly abnormal 4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cdk1 phosphorylates the conserved centriole protein Sas-4 during mitosis. This creates a Polo-docking site that helps recruit Polo to daughter centrioles.
Title: Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter Centrioles and Convert Them to Centrosomes.

Inactivation of the spindle assembly checkpoint in the sas-4 mutant impairs neuroblast proliferation and disrupts euploidy.
Title: Spindle assembly checkpoint inactivation fails to suppress neuroblast tumour formation in aurA mutant Drosophila.

The authors show here, however, that Cnn, Spd-2 and Asl are not recruited to the mother centriole as part of a complex with Sas-4.
Title: Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP.

Sas-4 acts as a vehicle to tether PCM complexes to centrioles independent of its well-known role in centriole duplication
Title: Conserved TCP domain of Sas-4/CPAP is essential for pericentriolar material tethering during centrosome biogenesis.

Data indicate that interphase centrioles are closely associated with Sas-4, Spd-2, Polo kinase, Pericentrin-like protein (Dplp), Asterless (Asl), Plk4 kinase, Centrosomin (Cnn) and gamma-tubulin.
Title: Structured illumination of the interface between centriole and peri-centriolar material.

The following Sas-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sas-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04166	NM_141444.2 Latest version!	Drosophila melanogaster spindle assembly abnormal 4 (Sas-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sas-4 ( NM_141444.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649701.2 Drosophila melanogaster spindle assembly abnormal 4 (Sas-4), mRNA.

Price & Availability↑

CloneID	OFa04166
Clone ID Related Accession (Same CDS sequence)	NM_141444.2
Accession Version	NM_141444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spindle assembly abnormal 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141444.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGAGG CTGGCGAAAG TCCTGTTGGA ATGCCTCTGA GCCAGGAGGC CATCGCACAG 
CGTCTCGCGG CGCTTAGTCG TTGGCAGGAC GAGCAGAAGC GGCTGCTGCA GGAGCGGCAG 
TCCAATCACC GCGTTTTGCT CGGCTTGGAG CAACGTAATA TGTACAAAAT GCTGGGACTG 
CTACACCAGG AAACGGAAAG CCATGAAAAC AGTGTGCTGG AGGAGTGGGA CGAGGAGCAC 
TCTATACAAA TGCCGCGACT CGCGGCGGAT CCGGACGAGC ACGAGCCAGA GATTTCAATG 
GCTGATCCCC AGCCGGCCAA GCCTAAACGC CCTTTTCTGC GTCGCGGCGA AGGTCTAAAG 
CAGCGGTTCA AGATCAATCC CGACCAGCTG CGCCTGGAAA ACCTGCCGCG ATACAAGTTC 
GCTAATGCCC ACCCTCAGTT CCGCACGCCT CAAATGAAAA AGGGTATTCT CAAGAAGCAA 
GTCAAGTCAC CTCCCAATCC TGCGCCTCCA CCAGCTCCCA AACCCTCAGC ACAGCTCGAC 
GATCTCAACG AGCAGCGCTT TCAGCAGATG CTTATCGGCA AGAACGCCTG CAGTTCCACT 
CCAGATCTAA AGTCTTCCTA TGCGTCCTCC ACAACCGCCT CGAGCACCTC GCCCAGAGTT 
CGTTTTGTAG AGCAGAAGAG CAGGGCGGGA CAAACCACTC ATGCCGATGA TGAAGCCTCC 
TCGGAAGCTA GTCCCATGAC AGGAGTTTGC TGGGCCAAGG TGCTGGACAC ACAGAGTATA 
AAGCCAGCGC AAATCCAAAG ACGCTCTGCT CAAATCCGAG TGGAAGATGA CAGCAATGTA 
AGCATATTCG AGCTGCTCGA GCAGAAGGCC ACTGAGGGAA ACATTGATAT GAACTCCAGC 
TGCATTCGAA CCTTTATGGC GCGCAAAGAT CAGCGTCGCC GAGTAGCTGA TGACTCCGAT 
CACATTGTAG TCACACAGCA GGTGCGTCAG ATGCGATTGC AGCCACAGGT GGACCAGATG 
CTGGTGCAGG AACTGCAGGA GGGGGACGAG GAGGACACCG AACCGTCCAG CGATCAGACC 
CTCACAATGA CACCCATCCA GGTGGGGAAT ACTAAAGTGC GAGTGCGTTT CTCCGACTCC 
AATGACACGC ACGAGTACTC CGATGCCACC AGCCTCAACG ACGGCTCCAA TATTCAGCTC 
TTTGAACAGT TCAAGTCCGC CCTCTTCCAG GCATTGGAAC AGAAGAAGAA GTCAAGTCCC 
AGCAAGGAGT CAGACGAACC CATAACCAAA GATCTTCAGG AGAAAGCCAA TCTCGTTCGC 
ACTCGCCTCG AAGAGCTGGA GACAGAGATA GCCACCTTCA AGGAGCAGAA TGCCCAACTC 
CTTCGCCTGA GGCAGCAACA CGAATTGGAG AAGGCAAAGT GCACACAGGA TCACATGGAG 
GCCATGGAAC GCGTCCATGA CGAAAAGATT CAAGCTGAAA TTTATCTGCA CGACGAGCGC 
ATGAAGATTG AGGAGGAACG ACGCAAGTTC GAGCAGCAAA TGCGACTTCA AAAGAGCAAT 
GCAAACAGCA AGGAAAAAAA GGAGATAGCC GCCTTGAAAC AGGAAGTGGA GGGACTGCAA 
CTCCAGCTCA AACAAAAGGA GCAGGCGCAT GTCTCGGCAC AGGCACGACT CCGCGCTCAA 
CTGCGGGCCA GTGAGAAGGA GCAGCGAAAC TACCGTGACG AGATCGAGCT GCTGCGCAAG 
GAGAACAAGC GCCTAGAGCA AGAGCTGGTC AAGATTGGCC GCGAAAACAA CAGCAAGATG 
CTGCAGGAGA TCAATCGAAA CATAGCCCGG CTTGCCCCCA AAGTGTTACC TTCCGCGACA 
ATGTCCGATA TCCTCGATGA GAACGGTCGG CGCACCCAAT CTCTGGACGG CACTGCTGGT 
AAGCCTGCCA AGCAGCGAGA GGCGCAAAAC CGTCGTCAGA GTAGCGGCAG TGCCCAAGTG 
AGAAGTCGGA GTCGCTCACT GAGCAGGAAT AAGAAAACAC CATACGCTCT GGATGAGAGC 
TACGCCTCTA GTAGTTCTGT GGAATCGGAG GAAGTTCAGC CGCCCGTGAC CGCTCCGAAA 
GCTGCTACCC CCCCACCGGC TGCCAACTCC AGCAGTGACT TTAAGCGGGA GATTACGAAT 
GCGGATGGGA GCAAGGACAT TTGGTACCCA AACGGCAATC TAAAAAAGAT AAGCGCCGAT 
GGCATGAACT TGCGCATGCT CTATTTCAAC AAGGATATCA AGGAAACTAA CATCAGGGAA 
GGCACAGTGA AGTATTACTA CGCTGAGACC AACACTTGGC ACACCTCCTA CCTAGATGGC 
TTGGAAATAC TCGAGTTTCC CAACGGGCAG ACGGAACATC GCCGCAAGGA TGGCACTGTT 
GAGATTCACT TTCCCAACAA CAGCATTAAG ATTGTGGATC CCAGCGATAC GGAGAAACTG 
GAGGAGTGGC GTTATGCGGA TGGAACCCAT CTAGTGCAGC TCCGGAATGG TGACAAGATT 
CTCAATCTGC CCAATGGCCA AAAGGAGATT CACACCAAAC TAAACAAGCG AAGGGAGTAT 
CCGGACGGCA CTGTGAAGCT AGTGTATCCG GATGGCAGCC AAGAGACGCG CTACTCCAAC 
GGCCGCGTTC GTTTGAAGGA CAAGGATGGC AAGCTCATAA TGGACACAGA CTATGCCAAG 
TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649701.2
CDS	42..2747
Translation	MQEAGESPVGMPLSQEAIAQRLAALSRWQDEQKRLLQERQSNHRVLLGLEQRNMYKMLGLLHQETESHENSVLEEWDEEHSIQMPRLAADPDEHEPEISMADPQPAKPKRPFLRRGEGLKQRFKINPDQLRLENLPRYKFANAHPQFRTPQMKKGILKKQVKSPPNPAPPPAPKPSAQLDDLNEQRFQQMLIGKNACSSTPDLKSSYASSTTASSTSPRVRFVEQKSRAGQTTHADDEASSEASPMTGVCWAKVLDTQSIKPAQIQRRSAQIRVEDDSNVSIFELLEQKATEGNIDMNSSCIRTFMARKDQRRRVADDSDHIVVTQQVRQMRLQPQVDQMLVQELQEGDEEDTEPSSDQTLTMTPIQVGNTKVRVRFSDSNDTHEYSDATSLNDGSNIQLFEQFKSALFQALEQKKKSSPSKESDEPITKDLQEKANLVRTRLEELETEIATFKEQNAQLLRLRQQHELEKAKCTQDHMEAMERVHDEKIQAEIYLHDERMKIEEERRKFEQQMRLQKSNANSKEKKEIAALKQEVEGLQLQLKQKEQAHVSAQARLRAQLRASEKEQRNYRDEIELLRKENKRLEQELVKIGRENNSKMLQEINRNIARLAPKVLPSATMSDILDENGRRTQSLDGTAGKPAKQREAQNRRQSSGSAQVRSRSRSLSRNKKTPYALDESYASSSSVESEEVQPPVTAPKAATPPPAANSSSDFKREITNADGSKDIWYPNGNLKKISADGMNLRMLYFNKDIKETNIREGTVKYYYAETNTWHTSYLDGLEILEFPNGQTEHRRKDGTVEIHFPNNSIKIVDPSDTEKLEEWRYADGTHLVQLRNGDKILNLPNGQKEIHTKLNKRREYPDGTVKLVYPDGSQETRYSNGRVRLKDKDGKLIMDTDYAKY

Target ORF information:

RefSeq Version	NM_141444.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spindle assembly abnormal 4 (Sas-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141444.2

ORF Insert Sequence:

ATGCAGGAGG CTGGCGAAAG TCCTGTTGGA ATGCCTCTGA GCCAGGAGGC CATCGCACAG 
CGTCTCGCGG CGCTTAGTCG TTGGCAGGAC GAGCAGAAGC GGCTGCTGCA GGAGCGGCAG 
TCCAATCACC GCGTTTTGCT CGGCTTGGAG CAACGTAATA TGTACAAAAT GCTGGGACTG 
CTACACCAGG AAACGGAAAG CCATGAAAAC AGTGTGCTGG AGGAGTGGGA CGAGGAGCAC 
TCTATACAAA TGCCGCGACT CGCGGCGGAT CCGGACGAGC ACGAGCCAGA GATTTCAATG 
GCTGATCCCC AGCCGGCCAA GCCTAAACGC CCTTTTCTGC GTCGCGGCGA AGGTCTAAAG 
CAGCGGTTCA AGATCAATCC CGACCAGCTG CGCCTGGAAA ACCTGCCGCG ATACAAGTTC 
GCTAATGCCC ACCCTCAGTT CCGCACGCCT CAAATGAAAA AGGGTATTCT CAAGAAGCAA 
GTCAAGTCAC CTCCCAATCC TGCGCCTCCA CCAGCTCCCA AACCCTCAGC ACAGCTCGAC 
GATCTCAACG AGCAGCGCTT TCAGCAGATG CTTATCGGCA AGAACGCCTG CAGTTCCACT 
CCAGATCTAA AGTCTTCCTA TGCGTCCTCC ACAACCGCCT CGAGCACCTC GCCCAGAGTT 
CGTTTTGTAG AGCAGAAGAG CAGGGCGGGA CAAACCACTC ATGCCGATGA TGAAGCCTCC 
TCGGAAGCTA GTCCCATGAC AGGAGTTTGC TGGGCCAAGG TGCTGGACAC ACAGAGTATA 
AAGCCAGCGC AAATCCAAAG ACGCTCTGCT CAAATCCGAG TGGAAGATGA CAGCAATGTA 
AGCATATTCG AGCTGCTCGA GCAGAAGGCC ACTGAGGGAA ACATTGATAT GAACTCCAGC 
TGCATTCGAA CCTTTATGGC GCGCAAAGAT CAGCGTCGCC GAGTAGCTGA TGACTCCGAT 
CACATTGTAG TCACACAGCA GGTGCGTCAG ATGCGATTGC AGCCACAGGT GGACCAGATG 
CTGGTGCAGG AACTGCAGGA GGGGGACGAG GAGGACACCG AACCGTCCAG CGATCAGACC 
CTCACAATGA CACCCATCCA GGTGGGGAAT ACTAAAGTGC GAGTGCGTTT CTCCGACTCC 
AATGACACGC ACGAGTACTC CGATGCCACC AGCCTCAACG ACGGCTCCAA TATTCAGCTC 
TTTGAACAGT TCAAGTCCGC CCTCTTCCAG GCATTGGAAC AGAAGAAGAA GTCAAGTCCC 
AGCAAGGAGT CAGACGAACC CATAACCAAA GATCTTCAGG AGAAAGCCAA TCTCGTTCGC 
ACTCGCCTCG AAGAGCTGGA GACAGAGATA GCCACCTTCA AGGAGCAGAA TGCCCAACTC 
CTTCGCCTGA GGCAGCAACA CGAATTGGAG AAGGCAAAGT GCACACAGGA TCACATGGAG 
GCCATGGAAC GCGTCCATGA CGAAAAGATT CAAGCTGAAA TTTATCTGCA CGACGAGCGC 
ATGAAGATTG AGGAGGAACG ACGCAAGTTC GAGCAGCAAA TGCGACTTCA AAAGAGCAAT 
GCAAACAGCA AGGAAAAAAA GGAGATAGCC GCCTTGAAAC AGGAAGTGGA GGGACTGCAA 
CTCCAGCTCA AACAAAAGGA GCAGGCGCAT GTCTCGGCAC AGGCACGACT CCGCGCTCAA 
CTGCGGGCCA GTGAGAAGGA GCAGCGAAAC TACCGTGACG AGATCGAGCT GCTGCGCAAG 
GAGAACAAGC GCCTAGAGCA AGAGCTGGTC AAGATTGGCC GCGAAAACAA CAGCAAGATG 
CTGCAGGAGA TCAATCGAAA CATAGCCCGG CTTGCCCCCA AAGTGTTACC TTCCGCGACA 
ATGTCCGATA TCCTCGATGA GAACGGTCGG CGCACCCAAT CTCTGGACGG CACTGCTGGT 
AAGCCTGCCA AGCAGCGAGA GGCGCAAAAC CGTCGTCAGA GTAGCGGCAG TGCCCAAGTG 
AGAAGTCGGA GTCGCTCACT GAGCAGGAAT AAGAAAACAC CATACGCTCT GGATGAGAGC 
TACGCCTCTA GTAGTTCTGT GGAATCGGAG GAAGTTCAGC CGCCCGTGAC CGCTCCGAAA 
GCTGCTACCC CCCCACCGGC TGCCAACTCC AGCAGTGACT TTAAGCGGGA GATTACGAAT 
GCGGATGGGA GCAAGGACAT TTGGTACCCA AACGGCAATC TAAAAAAGAT AAGCGCCGAT 
GGCATGAACT TGCGCATGCT CTATTTCAAC AAGGATATCA AGGAAACTAA CATCAGGGAA 
GGCACAGTGA AGTATTACTA CGCTGAGACC AACACTTGGC ACACCTCCTA CCTAGATGGC 
TTGGAAATAC TCGAGTTTCC CAACGGGCAG ACGGAACATC GCCGCAAGGA TGGCACTGTT 
GAGATTCACT TTCCCAACAA CAGCATTAAG ATTGTGGATC CCAGCGATAC GGAGAAACTG 
GAGGAGTGGC GTTATGCGGA TGGAACCCAT CTAGTGCAGC TCCGGAATGG TGACAAGATT 
CTCAATCTGC CCAATGGCCA AAAGGAGATT CACACCAAAC TAAACAAGCG AAGGGAGTAT 
CCGGACGGCA CTGTGAAGCT AGTGTATCCG GATGGCAGCC AAGAGACGCG CTACTCCAAC 
GGCCGCGTTC GTTTGAAGGA CAAGGATGGC AAGCTCATAA TGGACACAGA CTATGCCAAG 
TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sas-4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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