alphaTub84B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	alphaTub84B
Entrez Gene ID	40848
Full Name	alpha-Tubulin at 84B
Synonyms	1t,ALPHA 84C,Alpha-Tubulin84B,BEST:LD32507,CG1913,DM1alpha,DTA1,Dmel\CG1913,T,TBA1_DROME,Talpha1,Tub,Tub 84B,Tub1C,Tuba84B,Tubalpha1,a-tub84B,a1t,aTub84B,alpha-Tub,alpha-Tub84B,alpha-tub,alpha-tub84B,alpha-tubulin,alpha-tubulin 84B,alpha1,alpha1t,alpha1tub,alpha1tub84B,alpha84B,alphaT,alphaTUB,alphaTUB84B,alphaTub,alphaTub1,alphaTub84,alphat-1,alphatub,alphatub84B,anon-EST:Liang-1.59,anon-EST:Liang-2.30,chr3R:2914276..2914416,clone 1.59,clone 2.30,l(3)84Bd,l(3)g3,ms(3)nc33,nc33,tub,tubA84B,tubalpha1,tubulin
General protein information	Preferred Names alpha-Tubulin at 84B Names CG1913 gene product from transcript CG1913-RA CG1913-PA Tubulin-alpha1 alpha tubulin alpha tubulin 84B alpha-Tubulin alpha-Tubulin 84B alpha-tubulin 1 alpha-tubulin84B alpha1 tubulin alpha1-tubulin alphaT alphaTub84B-PA alphaTubulin 84B alphaTubulin84B alpha[[1]] tubulin alpha[[1]]-tubulin alphatubulin tubulin tubulin 1 alpha-chain tubulin 1alpha tubulin alpha1 tubulin alpha1 promoter tubulin(alpha1) tubulin1alpha tubulin84B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84B2-84B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057424.4	NP_476772.1	alpha-Tubulin at 84B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TUBA1C	XP_003640008.1
Bos taurus (cattle)	LOC100141266	XP_005206417.1
Mus musculus (house mouse)	Tuba1c	NP_033474.1
Gallus gallus (chicken)	LOC100857247	XP_004942898.1
Eremothecium gossypii	AGOS_AFR441C	NP_985988.1
Arabidopsis thaliana (thale cress)	TUA4	NP_171974.1
Pan troglodytes (chimpanzee)	TUBA1C	XP_509043.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010971	XP_309723.4
Schizosaccharomyces pombe (fission yeast)	nda2	NP_596774.1
Neurospora crassa	NCU09468	XP_963223.1
Homo sapiens (human)	TUBA1C	NP_116093.1
Rattus norvegicus (Norway rat)	Tuba1c	NP_001011995.1
Danio rerio (zebrafish)	tuba1b	NP_919369.2
Caenorhabditis elegans (roundworm)	tba-2	NP_001021050.1
Kluyveromyces lactis	KLLA0C16874g	XP_452955.1
Arabidopsis thaliana (thale cress)	TUA6	NP_193232.1
Oryza sativa (rice)	Os03g0726100	NP_001051132.1
	LOC710110	XP_002808026.1
Rattus norvegicus (Norway rat)	LOC100909441	XP_003749402.1
Drosophila melanogaster (fruit fly)	alphaTub84B	NP_476772.1
Caenorhabditis elegans (roundworm)	tba-4	NP_496351.1
Caenorhabditis elegans (roundworm)	tba-1	NP_001251213.1
Schizosaccharomyces pombe (fission yeast)	atb2	NP_595106.1
Magnaporthe oryzae (rice blast fungus)	MGG_06650	XP_003709356.1
Oryza sativa (rice)	Os11g0247300	NP_001067613.1
Bos taurus (cattle)	TUBA1C	NP_001029376.1
Drosophila melanogaster (fruit fly)	alphaTub84D	NP_524264.1
Saccharomyces cerevisiae (baker's yeast)	TUB1	NP_013625.1
Arabidopsis thaliana (thale cress)	TUA2	NP_175423.1
Xenopus tropicalis (tropical clawed frog)	tuba1c	NP_989078.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

human RITA, binding to Drosophila Su(H) and tubulin, cannot influence the Notch signalling pathway in the fly
Title: Analysis of the interaction between human RITA and Drosophila Suppressor of Hairless.

The following alphaTub84B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alphaTub84B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04151	NM_057424.4 Latest version!	Drosophila melanogaster alpha-Tubulin at 84B (alphaTub84B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alphaTub84B ( NM_057424.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476772.1 Drosophila melanogaster alpha-Tubulin at 84B (alphaTub84B), mRNA.

Price & Availability↑

CloneID	OFa04151
Clone ID Related Accession (Same CDS sequence)	NM_057424.4
Accession Version	NM_057424.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha-Tubulin at 84B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057424.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTGAAT GTATCTCTAT CCATGTTGGT CAGGCTGGTG TCCAGATTGG AAACGCCTGC 
TGGGAGCTCT ACTGCTTGGA GCACGGCATC CAGCCCGATG GCCAGATGCC GTCTGACAAG 
ACCGTGGGCG GAGGTGATGA CTCGTTCAAC ACCTTCTTCA GCGAGACTGG AGCTGGCAAG 
CACGTGCCCC GCGCCGTGTT TGTGGATCTG GAACCCACTG TGGTCGATGA GGTCCGTACC 
GGAACCTACC GTCAGCTGTT CCACCCCGAG CAGCTGATCA CTGGTAAGGA GGATGCGGCC 
AACAACTACG CCCGTGGCCA CTACACCATC GGCAAGGAGA TCGTCGATCT GGTTCTGGAC 
AGGATCCGCA AGCTGGCCGA TCAGTGCACC GGTCTGCAGG GCTTCCTCAT CTTCCACTCG 
TTCGGTGGAG GTACCGGCTC CGGCTTCACC TCGCTGCTGA TGGAGCGTCT CTCCGTGGAC 
TACGGCAAGA AGTCCAAGCT GGAGTTCGCC ATCTACCCAG CCCCCCAGGT GTCCACTGCC 
GTGGTCGAGC CCTACAACTC CATCCTGACC ACCCACACCA CCCTGGAGCA TTCCGACTGC 
GCCTTCATGG TCGACAACGA GGCTATCTAC GACATCTGCC GCCGCAATCT GGACATTGAG 
CGCCCCACGT ACACCAACCT GAACCGTCTG ATTGGCCAGA TCGTGTCCTC GATTACCGCC 
TCTCTGCGAT TCGATGGTGC CCTTAACGTG GATCTGACTG AGTTCCAGAC CAACTTGGTG 
CCCTACCCAC GTATTCACTT CCCTCTGGTG ACCTACGCCC CCGTTATCTC CGCCGAGAAG 
GCCTACCACG AGCAGCTGTC GGTGGCTGAG ATCACCAACG CCTGCTTCGA GCCGGCCAAC 
CAGATGGTCA AGTGCGATCC CCGTCACGGC AAGTACATGG CCTGCTGCAT GCTGTACCGC 
GGTGATGTTG TGCCCAAGGA CGTCAACGCC GCTATTGCCA CCATCAAGAC CAAGCGCACC 
ATTCAATTCG TCGACTGGTG CCCCACTGGC TTCAAGGTTG GCATCAACTA CCAGCCACCC 
ACCGTGGTGC CTGGAGGTGA TTTGGCCAAG GTGCAGCGTG CCGTGTGCAT GTTGTCCAAC 
ACCACGGCCA TCGCCGAGGC CTGGGCCCGT CTGGACCACA AGTTCGATCT GATGTACGCC 
AAGCGTGCCT TCGTCCACTG GTACGTTGGT GAGGGTATGG AGGAGGGAGA GTTCTCCGAG 
GCCCGTGAGG ATTTGGCTGC CCTCGAGAAG GACTACGAGG AGGTCGGCAT GGACTCCGGT 
GACGGCGAGG GTGAGGGCGC TGAGGAGTAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476772.1
CDS	144..1496
Translation	MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGMDSGDGEGEGAEEY

Target ORF information:

RefSeq Version	NM_057424.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha-Tubulin at 84B (alphaTub84B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057424.4

ORF Insert Sequence:

ATGCGTGAAT GTATCTCTAT CCATGTTGGT CAGGCTGGTG TCCAGATTGG AAACGCCTGC 
TGGGAGCTCT ACTGCTTGGA GCACGGCATC CAGCCCGATG GCCAGATGCC GTCTGACAAG 
ACCGTGGGCG GAGGTGATGA CTCGTTCAAC ACCTTCTTCA GCGAGACTGG AGCTGGCAAG 
CACGTGCCCC GCGCCGTGTT TGTGGATCTG GAACCCACTG TGGTCGATGA GGTCCGTACC 
GGAACCTACC GTCAGCTGTT CCACCCCGAG CAGCTGATCA CTGGTAAGGA GGATGCGGCC 
AACAACTACG CCCGTGGCCA CTACACCATC GGCAAGGAGA TCGTCGATCT GGTTCTGGAC 
AGGATCCGCA AGCTGGCCGA TCAGTGCACC GGTCTGCAGG GCTTCCTCAT CTTCCACTCG 
TTCGGTGGAG GTACCGGCTC CGGCTTCACC TCGCTGCTGA TGGAGCGTCT CTCCGTGGAC 
TACGGCAAGA AGTCCAAGCT GGAGTTCGCC ATCTACCCAG CCCCCCAGGT GTCCACTGCC 
GTGGTCGAGC CCTACAACTC CATCCTGACC ACCCACACCA CCCTGGAGCA TTCCGACTGC 
GCCTTCATGG TCGACAACGA GGCTATCTAC GACATCTGCC GCCGCAATCT GGACATTGAG 
CGCCCCACGT ACACCAACCT GAACCGTCTG ATTGGCCAGA TCGTGTCCTC GATTACCGCC 
TCTCTGCGAT TCGATGGTGC CCTTAACGTG GATCTGACTG AGTTCCAGAC CAACTTGGTG 
CCCTACCCAC GTATTCACTT CCCTCTGGTG ACCTACGCCC CCGTTATCTC CGCCGAGAAG 
GCCTACCACG AGCAGCTGTC GGTGGCTGAG ATCACCAACG CCTGCTTCGA GCCGGCCAAC 
CAGATGGTCA AGTGCGATCC CCGTCACGGC AAGTACATGG CCTGCTGCAT GCTGTACCGC 
GGTGATGTTG TGCCCAAGGA CGTCAACGCC GCTATTGCCA CCATCAAGAC CAAGCGCACC 
ATTCAATTCG TCGACTGGTG CCCCACTGGC TTCAAGGTTG GCATCAACTA CCAGCCACCC 
ACCGTGGTGC CTGGAGGTGA TTTGGCCAAG GTGCAGCGTG CCGTGTGCAT GTTGTCCAAC 
ACCACGGCCA TCGCCGAGGC CTGGGCCCGT CTGGACCACA AGTTCGATCT GATGTACGCC 
AAGCGTGCCT TCGTCCACTG GTACGTTGGT GAGGGTATGG AGGAGGGAGA GTTCTCCGAG 
GCCCGTGAGG ATTTGGCTGC CCTCGAGAAG GACTACGAGG AGGTCGGCAT GGACTCCGGT 
GACGGCGAGG GTGAGGGCGC TGAGGAGTAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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alphaTub84B cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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