Ufl1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ufl1
Entrez Gene ID	40843
Full Name	UFM1 specific ligase 1
Synonyms	CG1104,Dmel\CG1104,UFL1
General protein information	Preferred Names UFM1 specific ligase 1 Names CG1104 gene product from transcript CG1104-RC CG1104-PA CG1104-PC Ufl1-PA Ufl1-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84B2-84B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260028.2	NP_001246957.1	UFM1 specific ligase 1, isoform C
NM_141433.3	NP_649690.1	UFM1 specific ligase 1, isoform A

Pathways from BioSystems

Homologies

	UFL1	XP_001100312.1
Canis lupus familiaris (dog)	UFL1	XP_532236.4
Bos taurus (cattle)	UFL1	NP_001073745.1
Anopheles gambiae (African malaria mosquito)	UFL1_ANOGA	XP_317778.3
Arabidopsis thaliana (thale cress)	AT3G46220	NP_566883.4
Homo sapiens (human)	UFL1	NP_056138.1
Gallus gallus (chicken)	UFL1	NP_001096671.1
Mus musculus (house mouse)	Ufl1	NP_080470.2
Rattus norvegicus (Norway rat)	Ufl1	NP_001119751.1
Drosophila melanogaster (fruit fly)	CG1104	NP_001246957.1
Oryza sativa (rice)	Os05g0117600	NP_001174184.1
Xenopus tropicalis (tropical clawed frog)	ufl1	XP_002934919.2
Pan troglodytes (chimpanzee)	UFL1	XP_518643.2
Danio rerio (zebrafish)	ufl1	NP_956900.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ufl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ufl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04146	NM_141433.3 Latest version!	Drosophila melanogaster UFM1 specific ligase 1 (Ufl1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa04146	NM_001260028.2 Latest version!	Drosophila melanogaster UFM1 specific ligase 1 (Ufl1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ufl1 ( NM_001260028.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246957.1 Drosophila melanogaster UFM1 specific ligase 1 (Ufl1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04146
Clone ID Related Accession (Same CDS sequence)	NM_001260028.2 , NM_141433.3
Accession Version	NM_001260028.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	UFM1 specific ligase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260028.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAGTG ACTGGGACGA GATCAAGCGC TTGGCCGCCG ACTTTCAGAA GGCGCAGCTC 
ACGTCTACGC TGCAGAAGCT CTCGGAGCGA AACTGCGTGG AGATCGTCAC GCTGCTGCTG 
GAGAAGCAGA TGCTGGAGGT GGTGTTCACC AACGATGGCA AGGAGTACAT CACGCCGGAT 
CATCTGGAGC GCGAGATCCA GGACGAGTTG TACGTGAACG GAGGTCGTGC CAACTTGGTG 
GAGGTCAGCA AAACCCTAAA TGTGGATTTA TCACGAATCG AGCTGCTTGC CGAACGAATA 
TCTGCGGAGA ATCAAAGCGT ACACCTGGTA CTGGGACAGC TAATCGACGA GGACTACATC 
AGTCATATTG CGCAGGAGAT CAACGAGAAG CTGGTTCAGC GAGGAGAGGT TTCCATTTCG 
GAATTGGCTT CCCAGTTCGA TCTGCCGTCG GACTTCCTGC AACACGACGT GGTCGAAAAG 
CATCTAGGTA AGATTATTAA GGGTCGCCAG GATGCCTCAA ATCCGCGGGT GTTCTTCACT 
CAGGCCTACA TCCAACGTTG CAAAGCGAAG ATTCGGGGAG CCCTGGCCGC CATTACCAGG 
CCCATAAATG TGGCTGTGAT TCTTCAGAAG ATCGGTGTGC AGGAGAAGAT CTTCTACTCG 
TTGCTGGACG AAATCGCGCC AGCTGGCCAG GTTACCTCCA AGCAGGCCAA CTCCCAATAC 
GTGCCGCACA TCTATGCCAA GACGCAGGCT GACTGGGTGA ACTCGTTCTA CAAGCAGAAC 
AGCTTCCTTG AGTATGATGC CATTCAAAAG CTGGGAATTT CGGATGCCAA GTCCTACATT 
CGCAAGCAGT TCCCCAACGA AGAGTTTCTC TACCTCAAGC GTGTGGCCCT TGGCGCTCGA 
CTGGTGGAGC TCACGGTGGT CACGGCGCTA AATGAGTGCA GTGCCACCAA GCAGTACCTG 
GATCTGACCA CCATACTTCC ATCGAATCTG TCCGAGGAGG ATATCGAGGA GGTTTTCAGC 
ACCATCATGG CCCAAAAGCA TAGCAATCCC AGCAACTTTG TTTATCTTGA TGGCATTGTA 
TTTTCGCAAC CATACCTCGC GCAATTGGTT CAGCCGTGTC AAGCGTTGGC AGAGTCTCAG 
GCCAAGGCGG CCATCGATGG CGGCGTATAC CAGCAGTACA TTGTGGAGAA GACGCTAGCG 
CAAAAGGGAA ACGTGTCGAC CCAAGAGCTA GAGGACGAAG GCAAGGTGGA CAAGCGCGAC 
GAGCGGCGAA AGAAGGCGTC CTCTGGCAAA GCAGGAGGAG GAGCCCAAGG GCGTGAGACC 
AAGACCAAGT CCACGAAGAA GCATCAACGC GGAAAGGCGG CAGCTCAGTT CGACAGTGAT 
GATGAGGACG ATGCACAGCA AGGCTCTCGA GGTGGCGGTG GAGCAAGCAA AAAGGCAGTT 
AAGCCATTGG AGCTAGTCAA GACAGCGGAC ATCGTCAAAC TGATTACTGC GAGTCTGGAA 
GAGGAGGGAC TGGAGCATTT GTCGAAATCC ATTGCTTCCC TCTATACAAA CCAATTTAAT 
CAGACGGCCC TGGCACGTGC TCAGGAGTTG TTCGAGGCTA CACCGCAGAC AAATCGTCGC 
CAGACACACG CTGCTATCCA GGATCGCATC AATACGCTTT TGATAGACAT AAGGCTCTAT 
GAGAAGGGCT TAAAGCTTTT TCCGCAGGAC ACGCAAACCC AGTTGGTTAA ATACTTGCTA 
AAGTCATTGG GAAATGAGAT TTGCAACGAA CTTTCGCTAT ACGTGGCCAG CGAGTGCAAT 
CTGACTGTGA AAAATACCAA CCTGAATGTG GACCAGCGCA ATAAGTTGGC TCAGGAGTGC 
GAGGCCCAAT ACCGTGCCGC GTTGCTTGAG CAAAACAAAG CCCTGAATAA GAGCATTGAC 
GACTTTGAAC TGGCGACCGA AACGGTGCTG AAGACCTGCA GCATGATCAT CAAGAAGGTG 
GACAAGAAAA AGGATCGCCT GCTCATCGCA GACCACAAAA AGAAGCTGCA GAAGCAGCTA 
CTAGAGTGCA ACGAGCCGGC ACTGCTGCTG CACCTGGCTG CACTGATCTT GTTCACCACG 
ATCACTGGTA GCATTTTGCA CGCGTCCGGA AAATTCGTTT CTGCCATACT GCAGCATATT 
CGCGGCTCAC TAAACGAGGA CCAGAATGCT CTGTTGCTTC GGTATCACGA TTTGGTCCTG 
CAAGTTCTGC AGGCCATTCC CGATAGCAAT GAGTCAAAAC TGGCCAACGA ACATTTGCAG 
ACAATGCAGA ATCAAGTGGT GGAGCTAGCT CAAAACTTTT CACGTGCATC GATTTCTAAG 
GCTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246957.1
CDS	36..2384
Translation	MGSDWDEIKRLAADFQKAQLTSTLQKLSERNCVEIVTLLLEKQMLEVVFTNDGKEYITPDHLEREIQDELYVNGGRANLVEVSKTLNVDLSRIELLAERISAENQSVHLVLGQLIDEDYISHIAQEINEKLVQRGEVSISELASQFDLPSDFLQHDVVEKHLGKIIKGRQDASNPRVFFTQAYIQRCKAKIRGALAAITRPINVAVILQKIGVQEKIFYSLLDEIAPAGQVTSKQANSQYVPHIYAKTQADWVNSFYKQNSFLEYDAIQKLGISDAKSYIRKQFPNEEFLYLKRVALGARLVELTVVTALNECSATKQYLDLTTILPSNLSEEDIEEVFSTIMAQKHSNPSNFVYLDGIVFSQPYLAQLVQPCQALAESQAKAAIDGGVYQQYIVEKTLAQKGNVSTQELEDEGKVDKRDERRKKASSGKAGGGAQGRETKTKSTKKHQRGKAAAQFDSDDEDDAQQGSRGGGGASKKAVKPLELVKTADIVKLITASLEEEGLEHLSKSIASLYTNQFNQTALARAQELFEATPQTNRRQTHAAIQDRINTLLIDIRLYEKGLKLFPQDTQTQLVKYLLKSLGNEICNELSLYVASECNLTVKNTNLNVDQRNKLAQECEAQYRAALLEQNKALNKSIDDFELATETVLKTCSMIIKKVDKKKDRLLIADHKKKLQKQLLECNEPALLLHLAALILFTTITGSILHASGKFVSAILQHIRGSLNEDQNALLLRYHDLVLQVLQAIPDSNESKLANEHLQTMQNQVVELAQNFSRASISKAD

Target ORF information:

RefSeq Version	NM_001260028.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster UFM1 specific ligase 1 (Ufl1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260028.2

ORF Insert Sequence:

ATGGGTAGTG ACTGGGACGA GATCAAGCGC TTGGCCGCCG ACTTTCAGAA GGCGCAGCTC 
ACGTCTACGC TGCAGAAGCT CTCGGAGCGA AACTGCGTGG AGATCGTCAC GCTGCTGCTG 
GAGAAGCAGA TGCTGGAGGT GGTGTTCACC AACGATGGCA AGGAGTACAT CACGCCGGAT 
CATCTGGAGC GCGAGATCCA GGACGAGTTG TACGTGAACG GAGGTCGTGC CAACTTGGTG 
GAGGTCAGCA AAACCCTAAA TGTGGATTTA TCACGAATCG AGCTGCTTGC CGAACGAATA 
TCTGCGGAGA ATCAAAGCGT ACACCTGGTA CTGGGACAGC TAATCGACGA GGACTACATC 
AGTCATATTG CGCAGGAGAT CAACGAGAAG CTGGTTCAGC GAGGAGAGGT TTCCATTTCG 
GAATTGGCTT CCCAGTTCGA TCTGCCGTCG GACTTCCTGC AACACGACGT GGTCGAAAAG 
CATCTAGGTA AGATTATTAA GGGTCGCCAG GATGCCTCAA ATCCGCGGGT GTTCTTCACT 
CAGGCCTACA TCCAACGTTG CAAAGCGAAG ATTCGGGGAG CCCTGGCCGC CATTACCAGG 
CCCATAAATG TGGCTGTGAT TCTTCAGAAG ATCGGTGTGC AGGAGAAGAT CTTCTACTCG 
TTGCTGGACG AAATCGCGCC AGCTGGCCAG GTTACCTCCA AGCAGGCCAA CTCCCAATAC 
GTGCCGCACA TCTATGCCAA GACGCAGGCT GACTGGGTGA ACTCGTTCTA CAAGCAGAAC 
AGCTTCCTTG AGTATGATGC CATTCAAAAG CTGGGAATTT CGGATGCCAA GTCCTACATT 
CGCAAGCAGT TCCCCAACGA AGAGTTTCTC TACCTCAAGC GTGTGGCCCT TGGCGCTCGA 
CTGGTGGAGC TCACGGTGGT CACGGCGCTA AATGAGTGCA GTGCCACCAA GCAGTACCTG 
GATCTGACCA CCATACTTCC ATCGAATCTG TCCGAGGAGG ATATCGAGGA GGTTTTCAGC 
ACCATCATGG CCCAAAAGCA TAGCAATCCC AGCAACTTTG TTTATCTTGA TGGCATTGTA 
TTTTCGCAAC CATACCTCGC GCAATTGGTT CAGCCGTGTC AAGCGTTGGC AGAGTCTCAG 
GCCAAGGCGG CCATCGATGG CGGCGTATAC CAGCAGTACA TTGTGGAGAA GACGCTAGCG 
CAAAAGGGAA ACGTGTCGAC CCAAGAGCTA GAGGACGAAG GCAAGGTGGA CAAGCGCGAC 
GAGCGGCGAA AGAAGGCGTC CTCTGGCAAA GCAGGAGGAG GAGCCCAAGG GCGTGAGACC 
AAGACCAAGT CCACGAAGAA GCATCAACGC GGAAAGGCGG CAGCTCAGTT CGACAGTGAT 
GATGAGGACG ATGCACAGCA AGGCTCTCGA GGTGGCGGTG GAGCAAGCAA AAAGGCAGTT 
AAGCCATTGG AGCTAGTCAA GACAGCGGAC ATCGTCAAAC TGATTACTGC GAGTCTGGAA 
GAGGAGGGAC TGGAGCATTT GTCGAAATCC ATTGCTTCCC TCTATACAAA CCAATTTAAT 
CAGACGGCCC TGGCACGTGC TCAGGAGTTG TTCGAGGCTA CACCGCAGAC AAATCGTCGC 
CAGACACACG CTGCTATCCA GGATCGCATC AATACGCTTT TGATAGACAT AAGGCTCTAT 
GAGAAGGGCT TAAAGCTTTT TCCGCAGGAC ACGCAAACCC AGTTGGTTAA ATACTTGCTA 
AAGTCATTGG GAAATGAGAT TTGCAACGAA CTTTCGCTAT ACGTGGCCAG CGAGTGCAAT 
CTGACTGTGA AAAATACCAA CCTGAATGTG GACCAGCGCA ATAAGTTGGC TCAGGAGTGC 
GAGGCCCAAT ACCGTGCCGC GTTGCTTGAG CAAAACAAAG CCCTGAATAA GAGCATTGAC 
GACTTTGAAC TGGCGACCGA AACGGTGCTG AAGACCTGCA GCATGATCAT CAAGAAGGTG 
GACAAGAAAA AGGATCGCCT GCTCATCGCA GACCACAAAA AGAAGCTGCA GAAGCAGCTA 
CTAGAGTGCA ACGAGCCGGC ACTGCTGCTG CACCTGGCTG CACTGATCTT GTTCACCACG 
ATCACTGGTA GCATTTTGCA CGCGTCCGGA AAATTCGTTT CTGCCATACT GCAGCATATT 
CGCGGCTCAC TAAACGAGGA CCAGAATGCT CTGTTGCTTC GGTATCACGA TTTGGTCCTG 
CAAGTTCTGC AGGCCATTCC CGATAGCAAT GAGTCAAAAC TGGCCAACGA ACATTTGCAG 
ACAATGCAGA ATCAAGTGGT GGAGCTAGCT CAAAACTTTT CACGTGCATC GATTTCTAAG 
GCTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ufl1 ( NM_141433.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649690.1 Drosophila melanogaster UFM1 specific ligase 1 (Ufl1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04146
Clone ID Related Accession (Same CDS sequence)	NM_001260028.2 , NM_141433.3
Accession Version	NM_141433.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	UFM1 specific ligase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141433.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAGTG ACTGGGACGA GATCAAGCGC TTGGCCGCCG ACTTTCAGAA GGCGCAGCTC 
ACGTCTACGC TGCAGAAGCT CTCGGAGCGA AACTGCGTGG AGATCGTCAC GCTGCTGCTG 
GAGAAGCAGA TGCTGGAGGT GGTGTTCACC AACGATGGCA AGGAGTACAT CACGCCGGAT 
CATCTGGAGC GCGAGATCCA GGACGAGTTG TACGTGAACG GAGGTCGTGC CAACTTGGTG 
GAGGTCAGCA AAACCCTAAA TGTGGATTTA TCACGAATCG AGCTGCTTGC CGAACGAATA 
TCTGCGGAGA ATCAAAGCGT ACACCTGGTA CTGGGACAGC TAATCGACGA GGACTACATC 
AGTCATATTG CGCAGGAGAT CAACGAGAAG CTGGTTCAGC GAGGAGAGGT TTCCATTTCG 
GAATTGGCTT CCCAGTTCGA TCTGCCGTCG GACTTCCTGC AACACGACGT GGTCGAAAAG 
CATCTAGGTA AGATTATTAA GGGTCGCCAG GATGCCTCAA ATCCGCGGGT GTTCTTCACT 
CAGGCCTACA TCCAACGTTG CAAAGCGAAG ATTCGGGGAG CCCTGGCCGC CATTACCAGG 
CCCATAAATG TGGCTGTGAT TCTTCAGAAG ATCGGTGTGC AGGAGAAGAT CTTCTACTCG 
TTGCTGGACG AAATCGCGCC AGCTGGCCAG GTTACCTCCA AGCAGGCCAA CTCCCAATAC 
GTGCCGCACA TCTATGCCAA GACGCAGGCT GACTGGGTGA ACTCGTTCTA CAAGCAGAAC 
AGCTTCCTTG AGTATGATGC CATTCAAAAG CTGGGAATTT CGGATGCCAA GTCCTACATT 
CGCAAGCAGT TCCCCAACGA AGAGTTTCTC TACCTCAAGC GTGTGGCCCT TGGCGCTCGA 
CTGGTGGAGC TCACGGTGGT CACGGCGCTA AATGAGTGCA GTGCCACCAA GCAGTACCTG 
GATCTGACCA CCATACTTCC ATCGAATCTG TCCGAGGAGG ATATCGAGGA GGTTTTCAGC 
ACCATCATGG CCCAAAAGCA TAGCAATCCC AGCAACTTTG TTTATCTTGA TGGCATTGTA 
TTTTCGCAAC CATACCTCGC GCAATTGGTT CAGCCGTGTC AAGCGTTGGC AGAGTCTCAG 
GCCAAGGCGG CCATCGATGG CGGCGTATAC CAGCAGTACA TTGTGGAGAA GACGCTAGCG 
CAAAAGGGAA ACGTGTCGAC CCAAGAGCTA GAGGACGAAG GCAAGGTGGA CAAGCGCGAC 
GAGCGGCGAA AGAAGGCGTC CTCTGGCAAA GCAGGAGGAG GAGCCCAAGG GCGTGAGACC 
AAGACCAAGT CCACGAAGAA GCATCAACGC GGAAAGGCGG CAGCTCAGTT CGACAGTGAT 
GATGAGGACG ATGCACAGCA AGGCTCTCGA GGTGGCGGTG GAGCAAGCAA AAAGGCAGTT 
AAGCCATTGG AGCTAGTCAA GACAGCGGAC ATCGTCAAAC TGATTACTGC GAGTCTGGAA 
GAGGAGGGAC TGGAGCATTT GTCGAAATCC ATTGCTTCCC TCTATACAAA CCAATTTAAT 
CAGACGGCCC TGGCACGTGC TCAGGAGTTG TTCGAGGCTA CACCGCAGAC AAATCGTCGC 
CAGACACACG CTGCTATCCA GGATCGCATC AATACGCTTT TGATAGACAT AAGGCTCTAT 
GAGAAGGGCT TAAAGCTTTT TCCGCAGGAC ACGCAAACCC AGTTGGTTAA ATACTTGCTA 
AAGTCATTGG GAAATGAGAT TTGCAACGAA CTTTCGCTAT ACGTGGCCAG CGAGTGCAAT 
CTGACTGTGA AAAATACCAA CCTGAATGTG GACCAGCGCA ATAAGTTGGC TCAGGAGTGC 
GAGGCCCAAT ACCGTGCCGC GTTGCTTGAG CAAAACAAAG CCCTGAATAA GAGCATTGAC 
GACTTTGAAC TGGCGACCGA AACGGTGCTG AAGACCTGCA GCATGATCAT CAAGAAGGTG 
GACAAGAAAA AGGATCGCCT GCTCATCGCA GACCACAAAA AGAAGCTGCA GAAGCAGCTA 
CTAGAGTGCA ACGAGCCGGC ACTGCTGCTG CACCTGGCTG CACTGATCTT GTTCACCACG 
ATCACTGGTA GCATTTTGCA CGCGTCCGGA AAATTCGTTT CTGCCATACT GCAGCATATT 
CGCGGCTCAC TAAACGAGGA CCAGAATGCT CTGTTGCTTC GGTATCACGA TTTGGTCCTG 
CAAGTTCTGC AGGCCATTCC CGATAGCAAT GAGTCAAAAC TGGCCAACGA ACATTTGCAG 
ACAATGCAGA ATCAAGTGGT GGAGCTAGCT CAAAACTTTT CACGTGCATC GATTTCTAAG 
GCTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649690.1
CDS	128..2476
Translation	MGSDWDEIKRLAADFQKAQLTSTLQKLSERNCVEIVTLLLEKQMLEVVFTNDGKEYITPDHLEREIQDELYVNGGRANLVEVSKTLNVDLSRIELLAERISAENQSVHLVLGQLIDEDYISHIAQEINEKLVQRGEVSISELASQFDLPSDFLQHDVVEKHLGKIIKGRQDASNPRVFFTQAYIQRCKAKIRGALAAITRPINVAVILQKIGVQEKIFYSLLDEIAPAGQVTSKQANSQYVPHIYAKTQADWVNSFYKQNSFLEYDAIQKLGISDAKSYIRKQFPNEEFLYLKRVALGARLVELTVVTALNECSATKQYLDLTTILPSNLSEEDIEEVFSTIMAQKHSNPSNFVYLDGIVFSQPYLAQLVQPCQALAESQAKAAIDGGVYQQYIVEKTLAQKGNVSTQELEDEGKVDKRDERRKKASSGKAGGGAQGRETKTKSTKKHQRGKAAAQFDSDDEDDAQQGSRGGGGASKKAVKPLELVKTADIVKLITASLEEEGLEHLSKSIASLYTNQFNQTALARAQELFEATPQTNRRQTHAAIQDRINTLLIDIRLYEKGLKLFPQDTQTQLVKYLLKSLGNEICNELSLYVASECNLTVKNTNLNVDQRNKLAQECEAQYRAALLEQNKALNKSIDDFELATETVLKTCSMIIKKVDKKKDRLLIADHKKKLQKQLLECNEPALLLHLAALILFTTITGSILHASGKFVSAILQHIRGSLNEDQNALLLRYHDLVLQVLQAIPDSNESKLANEHLQTMQNQVVELAQNFSRASISKAD

Target ORF information:

RefSeq Version	NM_141433.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster UFM1 specific ligase 1 (Ufl1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141433.3

ORF Insert Sequence:

ATGGGTAGTG ACTGGGACGA GATCAAGCGC TTGGCCGCCG ACTTTCAGAA GGCGCAGCTC 
ACGTCTACGC TGCAGAAGCT CTCGGAGCGA AACTGCGTGG AGATCGTCAC GCTGCTGCTG 
GAGAAGCAGA TGCTGGAGGT GGTGTTCACC AACGATGGCA AGGAGTACAT CACGCCGGAT 
CATCTGGAGC GCGAGATCCA GGACGAGTTG TACGTGAACG GAGGTCGTGC CAACTTGGTG 
GAGGTCAGCA AAACCCTAAA TGTGGATTTA TCACGAATCG AGCTGCTTGC CGAACGAATA 
TCTGCGGAGA ATCAAAGCGT ACACCTGGTA CTGGGACAGC TAATCGACGA GGACTACATC 
AGTCATATTG CGCAGGAGAT CAACGAGAAG CTGGTTCAGC GAGGAGAGGT TTCCATTTCG 
GAATTGGCTT CCCAGTTCGA TCTGCCGTCG GACTTCCTGC AACACGACGT GGTCGAAAAG 
CATCTAGGTA AGATTATTAA GGGTCGCCAG GATGCCTCAA ATCCGCGGGT GTTCTTCACT 
CAGGCCTACA TCCAACGTTG CAAAGCGAAG ATTCGGGGAG CCCTGGCCGC CATTACCAGG 
CCCATAAATG TGGCTGTGAT TCTTCAGAAG ATCGGTGTGC AGGAGAAGAT CTTCTACTCG 
TTGCTGGACG AAATCGCGCC AGCTGGCCAG GTTACCTCCA AGCAGGCCAA CTCCCAATAC 
GTGCCGCACA TCTATGCCAA GACGCAGGCT GACTGGGTGA ACTCGTTCTA CAAGCAGAAC 
AGCTTCCTTG AGTATGATGC CATTCAAAAG CTGGGAATTT CGGATGCCAA GTCCTACATT 
CGCAAGCAGT TCCCCAACGA AGAGTTTCTC TACCTCAAGC GTGTGGCCCT TGGCGCTCGA 
CTGGTGGAGC TCACGGTGGT CACGGCGCTA AATGAGTGCA GTGCCACCAA GCAGTACCTG 
GATCTGACCA CCATACTTCC ATCGAATCTG TCCGAGGAGG ATATCGAGGA GGTTTTCAGC 
ACCATCATGG CCCAAAAGCA TAGCAATCCC AGCAACTTTG TTTATCTTGA TGGCATTGTA 
TTTTCGCAAC CATACCTCGC GCAATTGGTT CAGCCGTGTC AAGCGTTGGC AGAGTCTCAG 
GCCAAGGCGG CCATCGATGG CGGCGTATAC CAGCAGTACA TTGTGGAGAA GACGCTAGCG 
CAAAAGGGAA ACGTGTCGAC CCAAGAGCTA GAGGACGAAG GCAAGGTGGA CAAGCGCGAC 
GAGCGGCGAA AGAAGGCGTC CTCTGGCAAA GCAGGAGGAG GAGCCCAAGG GCGTGAGACC 
AAGACCAAGT CCACGAAGAA GCATCAACGC GGAAAGGCGG CAGCTCAGTT CGACAGTGAT 
GATGAGGACG ATGCACAGCA AGGCTCTCGA GGTGGCGGTG GAGCAAGCAA AAAGGCAGTT 
AAGCCATTGG AGCTAGTCAA GACAGCGGAC ATCGTCAAAC TGATTACTGC GAGTCTGGAA 
GAGGAGGGAC TGGAGCATTT GTCGAAATCC ATTGCTTCCC TCTATACAAA CCAATTTAAT 
CAGACGGCCC TGGCACGTGC TCAGGAGTTG TTCGAGGCTA CACCGCAGAC AAATCGTCGC 
CAGACACACG CTGCTATCCA GGATCGCATC AATACGCTTT TGATAGACAT AAGGCTCTAT 
GAGAAGGGCT TAAAGCTTTT TCCGCAGGAC ACGCAAACCC AGTTGGTTAA ATACTTGCTA 
AAGTCATTGG GAAATGAGAT TTGCAACGAA CTTTCGCTAT ACGTGGCCAG CGAGTGCAAT 
CTGACTGTGA AAAATACCAA CCTGAATGTG GACCAGCGCA ATAAGTTGGC TCAGGAGTGC 
GAGGCCCAAT ACCGTGCCGC GTTGCTTGAG CAAAACAAAG CCCTGAATAA GAGCATTGAC 
GACTTTGAAC TGGCGACCGA AACGGTGCTG AAGACCTGCA GCATGATCAT CAAGAAGGTG 
GACAAGAAAA AGGATCGCCT GCTCATCGCA GACCACAAAA AGAAGCTGCA GAAGCAGCTA 
CTAGAGTGCA ACGAGCCGGC ACTGCTGCTG CACCTGGCTG CACTGATCTT GTTCACCACG 
ATCACTGGTA GCATTTTGCA CGCGTCCGGA AAATTCGTTT CTGCCATACT GCAGCATATT 
CGCGGCTCAC TAAACGAGGA CCAGAATGCT CTGTTGCTTC GGTATCACGA TTTGGTCCTG 
CAAGTTCTGC AGGCCATTCC CGATAGCAAT GAGTCAAAAC TGGCCAACGA ACATTTGCAG 
ACAATGCAGA ATCAAGTGGT GGAGCTAGCT CAAAACTTTT CACGTGCATC GATTTCTAAG 
GCTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ufl1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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