Taf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Taf1
Entrez Gene ID	40813
Full Name	TBP-associated factor 1
Synonyms	BG:DS00004.13,CG17603,Dmel\CG17603,EfW1,SR3-5,TAF,TAF1,TAF200,TAF230,TAF250,TAF250/230,TAFII-250,TAFII250,TAF[II]250,TAF[[II]],TAF[[II]]230,TAF[[II]]250,TAF[[II]]250/230,TFIID,TFIID TAF250,Taf1p,Taf200,Taf230,Taf250,Taf[[II]]250,cel,cell,d230,dTAF1,dTAF230,dTAF250,dTAFII250,dTAF[[II]]230,dTAF[[II]]250,dmTAF1,dmTAF[[II]]230,l(3)84Ab,p230
General protein information	Preferred Names TBP-associated factor 1 Names CG17603 gene product from transcript CG17603-RE CG17603-PA CG17603-PB CG17603-PC CG17603-PD CG17603-PE CG17603-PF TATA-associated factor 250 TBP-associated factor TBP-associated factor 250 TBP-associated factor 250kD TFIID 230 kda subunit Taf1-PA Taf1-PB Taf1-PC Taf1-PD Taf1-PE Taf1-PF cell lethal suppressor of Ras85D 3-5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84A1-84A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057608.5	NP_476956.3	TBP-associated factor 1, isoform A
NM_206438.2	NP_996160.1	TBP-associated factor 1, isoform B
NM_001170061.1	NP_001163532.1	TBP-associated factor 1, isoform D
NM_001300272.1	NP_001287201.1	TBP-associated factor 1, isoform F
NM_206437.2	NP_996159.1	TBP-associated factor 1, isoform C
NM_001260023.1	NP_001246952.1	TBP-associated factor 1, isoform E

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003882	XP_308108.5
Xenopus tropicalis (tropical clawed frog)	taf1	XP_002934954.2
Canis lupus familiaris (dog)	TAF1	XP_005641531.1
Mus musculus (house mouse)	Taf1	NP_001074477.1
Danio rerio (zebrafish)	taf1	NP_001038250.1
Drosophila melanogaster (fruit fly)	Taf1	NP_996159.1
Caenorhabditis elegans (roundworm)	taf-1	NP_493426.2
Homo sapiens (human)	TAF1	NP_004597.2
Rattus norvegicus (Norway rat)	Taf1	NP_001178652.1
Bos taurus (cattle)	TAF1	XP_005228102.1
Gallus gallus (chicken)	TAF1	XP_420198.4
	TAF1	XP_002808585.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TAF1 is involved in both establishing an upper limit of transcription during induction and efficiently turning the gene off once the inducer is removed
Title: Holo-TFIID controls the magnitude of a transcription burst and fine-tuning of transcription.

The existence of a stable coactivator supercomplex consisting of the chromatin-remodeling factor Brahma (SWI/SNF) and the transcription initiation factor TFIID, is shown.
Title: Transcription coactivator SAYP combines chromatin remodeler Brahma and transcription initiation factor TFIID into a single supercomplex.

TAF1 pre-mRNA elements identified in this study are independently targeted by developmental signaling pathways to result in tissue-specific inclusion of exon 12a and/or 13a.
Title: A DNA damage signal activates and derepresses exon inclusion in Drosophila TAF1 alternative splicing.

results suggest stepwise assembly of a testis-specific TFIID complex (tTFIID) whereby a TAF1 isoform, presumably TAF1-2, is recruited to a core subassembly of tTAFs in spermatocyte nucleoli
Title: Nucleolar colocalization of TAF1 and testis-specific TAFs during Drosophila spermatogenesis.

The following Taf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Taf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04101	NM_206438.2 Latest version!	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa04102	NM_206437.2 Latest version!	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa04103	NM_057608.5 Latest version!	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa07570	NM_001260023.1 Latest version!	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa07571	NM_001170061.1 Latest version!	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa22059	NM_001300272.1 Latest version!	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Taf1 ( NM_206438.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996160.1 Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04101
Clone ID Related Accession (Same CDS sequence)	NM_206438.2
Accession Version	NM_206438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TBP-associated factor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_206438.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA CTAAGGACGA CAAGGAACTA ATGCCTCCAC CAAGTGCACC AATGCGCTCC 
GGCTCTGGCG GCGGCATCGA GGAACCGGCC AAGTCAAATG ATGCATCTAG TCCCAGTGAC 
GATTCCAAAT CTACCGATTC AAAGGATGCG GATCGGAAGC TGGACACACC ACTGGCAGAC 
ATACTGCCGT CCAAGTACCA GAATGTGGAT GTGCGTGAGC TCTTTCCGGA CTTTCGTCCC 
CAAAAGGTGC TACGCTTCTC CCGTCTCTTC GGACCCGGTA AACCCACGAG TTTGCCCCAG 
ATCTGGCGAC ACGTGCGCAA ACGCCGCCGC AAGCGAAATC AATCCAGGGA TCAGAAGACG 
ACAAACACTG GTGGTTCGGA CTCTCCAAGC GATACTGAGG AGCCACGAAA GCGGGGCTTC 
AGTCTGCACT ATGCTGCAGA GCCAACGCCA GCGGAGTGCA TGTCCGACGA CGAGGACAAA 
TTGCTGGGCG ACTTCAACAG CGAGGATGTG CGTCCAGAAG GACCGGACAA CGGCGAGAAC 
AGCGATCAGA AGCCAAAGGT GGCTGACTGG CGGTTCGGGC CTGCACAGAT TTGGTATGAT 
ATGCTAGAGG TGCCCGACTC TGGAGAGGGG TTCAACTATG GCTTCAAGAC AAAGGCAGCA 
AGCACCTCGT CTCAGCCGCA ACTCAAAGAT GAACGCCGTG TAAAGAGTCC AGAAGATGAT 
GTCGAAGATC CAAGCATTGC GGATGATGCC TTTCTCATGG TCTCCCAGCT GCATTGGGAA 
GACGACGTGG TCTGGGACGG TAACGACATC AAGGCAAAGG TGCTCCAAAA GCTTAACTCA 
AAGACAAATG CAGCCGGATG GTTGCCCTCG AGCGGGTCGA GAACGGCTGG CGCCTTTAGC 
CAGCCGGGCA AACCGTCTAT GCCCGTGGGA AGTGGAAGCT CCAAGCAGGG TTCGGGAGCA 
TCAAGCAAAA AGGCCCAGCA AAATGCTCAA GCAAAGCCAG CGGAGGCACC TGATGACACC 
TGGTATAGCC TTTTTCCAGT TGAAAATGAG GAGCTAATAT ACTACAAGTG GGAGGACGAG 
GTAATCTGGG ATGCCCAGCA AGTGAGCAAG GTGCCCAAGC CGAAGGTACT CACTTTGGAT 
CCCAACGACG AAAACATCAT TTTGGGTATT CCTGATGACA TAGATCCCTC TAAAATCAAT 
AAGAGCACGG GTCCTCCACC TAAGATCAAG ATACCGCATC CTCACGTAAA AAAGTCTAAG 
ATCCTACTTG GCAAGGCGGG TGTGATCAAC GTGCTTGCGG AGGACACACC TCCGCCGCCA 
CCCAAAAGTC CTGACCGTGA CCCCTTTAAC ATATCCAATG ACACTTACTA CACACCGAAG 
ACAGAACCCA CCCTACGACT TAAGGTGGGC GGTAATCTTA TTCAACACTC GACTCCGGTG 
GTAGAGCTGC GAGCTCCGTT TGTGCCTACG CACATGGGCC CGATGAAGCT CCGCGCCTTC 
CATCGCCCCC CCCTAAAGAA GTACTCCCAC GGGCCAATGG CCCAGTCTAT CCCTCATCCT 
GTCTTCCCAC TGCTGAAGAC CATCGCAAAG AAGGCGAAGC AGCGCGAAGT GGAGCGCATT 
GCCTCCGGTG GCGGAGACGT CTTCTTTATG CGCAACCCAG AGGATTTAAG CGGAAGGGAC 
GGAGACATCG TACTAGCCGA GTTCTGCGAA GAGCATCCGC CTCTGATAAA CCAGGTGGGC 
ATGTGCTCCA AGATAAAGAA CTACTACAAG CGTAAGGCTG AGAAGGACAG TGGGCCGCAG 
GATTTTGTTT ATGGAGAAGT GGCCTTTGCG CACACAAGTC CCTTCCTGGG CATCCTGCAT 
CCTGGCCAGT GCATACAGGC GATTGAGAAC AACATGTACC GAGCGCCTAT TTATCCACAT 
AAGATGGCTC ACAACGATTT CCTCGTCATT CGCACGCGGA ACAACTACTG GATACGGTCG 
GTGAATTCAA TATACACCGT GGGTCAGGAG TGTCCGCTGT ACGAGGTACC CGGTCCGAAT 
TCCAAAAGGG CCAACAACTT CACCCGTGAC TTTCTGCAGG TGTTTATTTA CCGCCTGTTC 
TGGAAAAGTC GCGACAACCC GCGCCGCATT CGAATGGACG ATATAAAACA GGCTTTTCCC 
GCTCATTCCG AGAGCAGCAT CCGCAAGCGT TTAAAGCAGT GCGCTGACTT CAAGCGAACA 
GGCATGGACT CCAATTGGTG GGTTATAAAG CCAGAGTTTC GCCTTCCATC CGAGGAGGAG 
ATCCGAGCCA TGGTGTCACC TGAGCAGTGT TGCGCGTACT TCAGCATGAT AGCGGCGGAA 
CAACGCTTAA AGGATGCTGG GTATGGAGAA AAGTTTTTGT TCGCACCTCA GGAAGATGAC 
GACGAGGAGG CGCAGCTGAA GCTTGACGAC GAAGTAAAGG TGGCTCCTTG GAACACGACT 
CGCGCATATA TCCAAGCCAT GCGGGGAAAG TGTTTACTCC AGTTGAGTGG TCCAGCCGAT 
CCAACGGGAT GTGGAGAGGG ATTTTCATAT GTTCGAGTGC CAAACAAGCC CACGCAAACC 
AAGGAGGAGC AAGAGTCGCA GCCTAAACGT TCGGTCACAG GAACAGATGC AGATTTGCGT 
CGTCTGCCAC TCCAGCGTGC AAAAGAGCTG TTGCGGCAGT TCAAGGTGCC CGAGGAGGAG 
ATCAAAAAGC TTTCCCGCTG GGAGGTCATT GACGTGGTGC GCACCCTGTC CACAGAAAAG 
GCCAAGGCCG GTGAAGAGGG AATGGATAAG TTTTCTCGTG GCAACCGGTT CTCCATTGCA 
GAGCATCAGG AGCGTTATAA GGAAGAGTGC CAGCGCATAT TCGATCTGCA AAACAGAGTG 
CTGGCCAGCT CTGAGGTGCT GTCCACAGAT GAGGCAGAGT CCTCGGCCTC TGAGGAATCT 
GATCTCGAAG AACTTGGCAA GAATCTTGAG AACATGCTGT CAAACAAGAA AACCTCGACG 
CAATTGTCAA GGGAACGTGA AGAGCTGGAG CGTCAGGAGT TGCTTCGCCA GCTTGACGAA 
GAACACGGCG GACCAAGTGG TAGTGGAGGA GCCAAGGGAG CCAAAGGAAA GGATGATCCG 
GGACAGCAAA TGCTGGCAAC CAACAACCAG GGCAGGATCC TTCGCATTAC GCGTACCTTT 
AGAGGTAACG ATGGCAAGGA ATATACTCGC GTGGAGACTG TGCGGCGGCA ACCAGTTATC 
GACGCCTACA TCAAGATTCG CACCACTAAG GACGAGCAGT TCATCAAGCA GTTCGCAACG 
CTAGATGAGC AGCAGAAGGA GGAGATGAAG CGCGAAAAGA GACGCATTCA GGAGCAGCTA 
CGTCGCATCA AGCGCAACCA GGAGCGCGAA CGCCTGGCGC AGCTGGCCCA GAACCAGAAG 
CTTCAGCCAG GTGGCATGCC CACTTCCTTG GGTGATCCTA AGAGCTCGGG CGGTCATTCG 
CACAAGGAGC GGGATAGCGG CTACAAGGAG GTCAGCCCTT CGCGCAAGAA GTTCAAGCTT 
AAGCCAGACC TAAAGCTGAA GTGCGGCGCC TGTGGACAGG TTGGTCACAT GCGCACAAAC 
AAAGCCTGTC CCTTGTATTC TGGCATGCAA AGCAGTCTGT CCCAGTCGAA CCCATCTCTG 
GCTGACGATT TTGACGAACA GAGCGAAAAG GAGATGACAA TGGATGACGA TGATCTTGTG 
AATGTCGATG GCACCAAAGT AACGCTCAGC AGTAAGATTC TCAAGCGTCA TGGTGGTGAT 
GATGGCAAGC GTCGCAGCGG ATCTAGCTCT GGTTTCACCT TGAAGGTTCC CCGAGATGCG 
ATGGGCAAGA AGAAACGCAG AGTGGGTGGC GATCTTCATT GTGACTATCT GCAGCGACAC 
AATAAAACGG CCAATCGCAG GCGCACGGAC CCCGTTGTGG TACTGTCCTC TATCCTGGAG 
ATTATCCATA ATGAGCTGCG ATCTATGCCA GATGTATCGC CATTCCTGTT CCCGGTAAGC 
GCAAAAAAGG TTCCCGACTA CTACCGCGTG GTGACCAAGC CCATGGATCT GCAAACGATG 
AGGGAGTATA TTCGCCAAAG GCGCTACACG AGTCGCGAGA TGTTCCTCGA GGATCTCAAG 
CAGATTGTGG ACAACTCGCT GATCTACAAT GGACCGCAGA GTGCATACAC CTTGGCTGCC 
CAACGCATGT TCAGCAGTTG TTTTGAATTG CTCGCAGAGC GCGAAGACAA ACTGATGCGC 
CTCGAGAAGG CAATTAACCC GCTGCTGGAC GACGATGACC AAGTGGCACT CTCCTTTATC 
TTTGACAAGC TGCACTCGCA GATTAAGCAA TTACCAGAGA GCTGGCCTTT CCTTAAGCCT 
GTCAACAAGA AACAGGTTAA GGACTACTAC ACGGTTATCA AGCGACCCAT GGACCTCGAA 
ACTATCGGCA AAAACATTGA AGCTCATCGC TATCACAGTC GTGCCGAGTA TCTGGCTGAT 
ATCGAGTTGA TCGCCACCAA CTGTGAGCAG TACAACGGCA GTGACACCCG CTACACCAAG 
TTCTCAAAGA AGATACTTGA GTATGCCCAA ACCCAGTTAA TTGAGTTTTC GGAGCACTGC 
GGCCAGTTGG AAAATAACAT AGCTAAGACG CAGGAGCGTG CTAGGGAAAA TGCACCAGAG 
TTTGATGAAG CCTGGGGCAA TGATGATTAC AACTTTGACC GTGGCAGTAG GGCCAGTTCA 
CCCGGAGATG ACTACATCGA CGTCGAGGGT CATGGGGGGC ATGCCTCCTC ATCGAACTCT 
ATCCATCGCA GCATGGGCGC CGAGGCCGGT TCGTCACATA CGGCGCCGGC GGTGCGAAAA 
CCAGCTCCTC CTGGTCCTGG TGAGGTGAAG CGCGGAAGGG GTAGGCCCCG CAAGCAGCGC 
GACCCCGTGG AGGAGGTCAA ATCCCAGAAT CCGGTTAAGC GTGGTCGGGG GCGTCCGAGG 
AAGGACAGCC TTGCCTCAAA CATGAGTCAC ACGCAAGCTT ACTTCCTGGA TGAAGATCTC 
CAATGCTCCA CAGATGACGA GGACGACGAC GAGGAGGAGG ACTTCCAGGA GGTCTCCGAA 
GACGAGAACA ATGCGGCGAG CATTTTAGAT CAGGGCGAAC GTATCAATGC GCCTGCCGAT 
GCCATGGATG GCATGTTTGA CCCCAAGAAC ATCAAGACAG AGATTGACCT AGAGGCTCAC 
CAGATGGCAG AGGAGCCGAT CGGCGAGGAT GACAGCCAGC AGGTGGCCGA AGCAATGGTG 
CAGTTGAGTG GCGTGGGCGG CTACTATGCT CAACAGCAGC AAGATGAATC CATGGATGTG 
GACCCCAACT ACGATCCCTC AGATTTCCTC GCCATGCACA AGCAGCGCCA GAGCCTCGGC 
GAGCCCAGCA GCTTGCAGGG TGCTTTCACC AACTTCCTAT CGCACGAGCA GGATGATAAT 
GGGCCTTACA ATCCCGCCGA AGCCAGCACA AGTGCCGCTT CCGGTGCAGA CTTAGGAATG 
GACGCTTCAA TGGCCATGCA AATGGCGCCG GAAATGCCTG TCAATACCAT GAACAACGGA 
ATGGGCATCG ATGATGATCT GGATATTTCG GAGAGTGACG AGGAAGACGA TGGTTCTCGA 
GTGCGTATCA AAAAGGAGGT CTTCGACGAC GGGGATTACG CCTTGCAGCA CCAGCAGATG 
GGACAGGCAG CATCGCAGTC GCAGATATAC ATGGTGGATT CGTCCAACGA GCCCACGACT 
CTCGACTACC AGCAACCACC GCAACTGGAC TTCCAACAAG TGCAGGAAAT GGAGCAGTTG 
CAGCACCAAG TGATGCCACC AATGCAATCA GAGCAACTGC AGCAGCAACA GACGCCGCAG 
GGAGACAATG ATTATGCCTG GACTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996160.1
CDS	102..6491
Translation	MEMESDNSDDEGSIGNGLDLTGILFGNIDSEGRLLQDDDGEGRGGTGFDAELRENIGSLSKLGLDSMLLEVIDLKEAEPPSDDEEEEDARPSAVSASEGMSAFDALKAGVKREDGAVKAQDDAIDYSDITELSEDCPRTPPEETSTYDDLEDAIPASKVEAKLTKDDKELMPPPSAPMRSGSGGGIEEPAKSNDASSPSDDSKSTDSKDADRKLDTPLADILPSKYQNVDVRELFPDFRPQKVLRFSRLFGPGKPTSLPQIWRHVRKRRRKRNQSRDQKTTNTGGSDSPSDTEEPRKRGFSLHYAAEPTPAECMSDDEDKLLGDFNSEDVRPEGPDNGENSDQKPKVADWRFGPAQIWYDMLEVPDSGEGFNYGFKTKAASTSSQPQLKDERRVKSPEDDVEDPSIADDAFLMVSQLHWEDDVVWDGNDIKAKVLQKLNSKTNAAGWLPSSGSRTAGAFSQPGKPSMPVGSGSSKQGSGASSKKAQQNAQAKPAEAPDDTWYSLFPVENEELIYYKWEDEVIWDAQQVSKVPKPKVLTLDPNDENIILGIPDDIDPSKINKSTGPPPKIKIPHPHVKKSKILLGKAGVINVLAEDTPPPPPKSPDRDPFNISNDTYYTPKTEPTLRLKVGGNLIQHSTPVVELRAPFVPTHMGPMKLRAFHRPPLKKYSHGPMAQSIPHPVFPLLKTIAKKAKQREVERIASGGGDVFFMRNPEDLSGRDGDIVLAEFCEEHPPLINQVGMCSKIKNYYKRKAEKDSGPQDFVYGEVAFAHTSPFLGILHPGQCIQAIENNMYRAPIYPHKMAHNDFLVIRTRNNYWIRSVNSIYTVGQECPLYEVPGPNSKRANNFTRDFLQVFIYRLFWKSRDNPRRIRMDDIKQAFPAHSESSIRKRLKQCADFKRTGMDSNWWVIKPEFRLPSEEEIRAMVSPEQCCAYFSMIAAEQRLKDAGYGEKFLFAPQEDDDEEAQLKLDDEVKVAPWNTTRAYIQAMRGKCLLQLSGPADPTGCGEGFSYVRVPNKPTQTKEEQESQPKRSVTGTDADLRRLPLQRAKELLRQFKVPEEEIKKLSRWEVIDVVRTLSTEKAKAGEEGMDKFSRGNRFSIAEHQERYKEECQRIFDLQNRVLASSEVLSTDEAESSASEESDLEELGKNLENMLSNKKTSTQLSREREELERQELLRQLDEEHGGPSGSGGAKGAKGKDDPGQQMLATNNQGRILRITRTFRGNDGKEYTRVETVRRQPVIDAYIKIRTTKDEQFIKQFATLDEQQKEEMKREKRRIQEQLRRIKRNQERERLAQLAQNQKLQPGGMPTSLGDPKSSGGHSHKERDSGYKEVSPSRKKFKLKPDLKLKCGACGQVGHMRTNKACPLYSGMQSSLSQSNPSLADDFDEQSEKEMTMDDDDLVNVDGTKVTLSSKILKRHGGDDGKRRSGSSSGFTLKVPRDAMGKKKRRVGGDLHCDYLQRHNKTANRRRTDPVVVLSSILEIIHNELRSMPDVSPFLFPVSAKKVPDYYRVVTKPMDLQTMREYIRQRRYTSREMFLEDLKQIVDNSLIYNGPQSAYTLAAQRMFSSCFELLAEREDKLMRLEKAINPLLDDDDQVALSFIFDKLHSQIKQLPESWPFLKPVNKKQVKDYYTVIKRPMDLETIGKNIEAHRYHSRAEYLADIELIATNCEQYNGSDTRYTKFSKKILEYAQTQLIEFSEHCGQLENNIAKTQERARENAPEFDEAWGNDDYNFDRGSRASSPGDDYIDVEGHGGHASSSNSIHRSMGAEAGSSHTAPAVRKPAPPGPGEVKRGRGRPRKQRDPVEEVKSQNPVKRGRGRPRKDSLASNMSHTQAYFLDEDLQCSTDDEDDDEEEDFQEVSEDENNAASILDQGERINAPADAMDGMFDPKNIKTEIDLEAHQMAEEPIGEDDSQQVAEAMVQLSGVGGYYAQQQQDESMDVDPNYDPSDFLAMHKQRQSLGEPSSLQGAFTNFLSHEQDDNGPYNPAEASTSAASGADLGMDASMAMQMAPEMPVNTMNNGMGIDDDLDISESDEEDDGSRVRIKKEVFDDGDYALQHQQMGQAASQSQIYMVDSSNEPTTLDYQQPPQLDFQQVQEMEQLQHQVMPPMQSEQLQQQQTPQGDNDYAWTF

Target ORF information:

RefSeq Version	NM_206438.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206438.2

ORF Insert Sequence:

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA CTAAGGACGA CAAGGAACTA ATGCCTCCAC CAAGTGCACC AATGCGCTCC 
GGCTCTGGCG GCGGCATCGA GGAACCGGCC AAGTCAAATG ATGCATCTAG TCCCAGTGAC 
GATTCCAAAT CTACCGATTC AAAGGATGCG GATCGGAAGC TGGACACACC ACTGGCAGAC 
ATACTGCCGT CCAAGTACCA GAATGTGGAT GTGCGTGAGC TCTTTCCGGA CTTTCGTCCC 
CAAAAGGTGC TACGCTTCTC CCGTCTCTTC GGACCCGGTA AACCCACGAG TTTGCCCCAG 
ATCTGGCGAC ACGTGCGCAA ACGCCGCCGC AAGCGAAATC AATCCAGGGA TCAGAAGACG 
ACAAACACTG GTGGTTCGGA CTCTCCAAGC GATACTGAGG AGCCACGAAA GCGGGGCTTC 
AGTCTGCACT ATGCTGCAGA GCCAACGCCA GCGGAGTGCA TGTCCGACGA CGAGGACAAA 
TTGCTGGGCG ACTTCAACAG CGAGGATGTG CGTCCAGAAG GACCGGACAA CGGCGAGAAC 
AGCGATCAGA AGCCAAAGGT GGCTGACTGG CGGTTCGGGC CTGCACAGAT TTGGTATGAT 
ATGCTAGAGG TGCCCGACTC TGGAGAGGGG TTCAACTATG GCTTCAAGAC AAAGGCAGCA 
AGCACCTCGT CTCAGCCGCA ACTCAAAGAT GAACGCCGTG TAAAGAGTCC AGAAGATGAT 
GTCGAAGATC CAAGCATTGC GGATGATGCC TTTCTCATGG TCTCCCAGCT GCATTGGGAA 
GACGACGTGG TCTGGGACGG TAACGACATC AAGGCAAAGG TGCTCCAAAA GCTTAACTCA 
AAGACAAATG CAGCCGGATG GTTGCCCTCG AGCGGGTCGA GAACGGCTGG CGCCTTTAGC 
CAGCCGGGCA AACCGTCTAT GCCCGTGGGA AGTGGAAGCT CCAAGCAGGG TTCGGGAGCA 
TCAAGCAAAA AGGCCCAGCA AAATGCTCAA GCAAAGCCAG CGGAGGCACC TGATGACACC 
TGGTATAGCC TTTTTCCAGT TGAAAATGAG GAGCTAATAT ACTACAAGTG GGAGGACGAG 
GTAATCTGGG ATGCCCAGCA AGTGAGCAAG GTGCCCAAGC CGAAGGTACT CACTTTGGAT 
CCCAACGACG AAAACATCAT TTTGGGTATT CCTGATGACA TAGATCCCTC TAAAATCAAT 
AAGAGCACGG GTCCTCCACC TAAGATCAAG ATACCGCATC CTCACGTAAA AAAGTCTAAG 
ATCCTACTTG GCAAGGCGGG TGTGATCAAC GTGCTTGCGG AGGACACACC TCCGCCGCCA 
CCCAAAAGTC CTGACCGTGA CCCCTTTAAC ATATCCAATG ACACTTACTA CACACCGAAG 
ACAGAACCCA CCCTACGACT TAAGGTGGGC GGTAATCTTA TTCAACACTC GACTCCGGTG 
GTAGAGCTGC GAGCTCCGTT TGTGCCTACG CACATGGGCC CGATGAAGCT CCGCGCCTTC 
CATCGCCCCC CCCTAAAGAA GTACTCCCAC GGGCCAATGG CCCAGTCTAT CCCTCATCCT 
GTCTTCCCAC TGCTGAAGAC CATCGCAAAG AAGGCGAAGC AGCGCGAAGT GGAGCGCATT 
GCCTCCGGTG GCGGAGACGT CTTCTTTATG CGCAACCCAG AGGATTTAAG CGGAAGGGAC 
GGAGACATCG TACTAGCCGA GTTCTGCGAA GAGCATCCGC CTCTGATAAA CCAGGTGGGC 
ATGTGCTCCA AGATAAAGAA CTACTACAAG CGTAAGGCTG AGAAGGACAG TGGGCCGCAG 
GATTTTGTTT ATGGAGAAGT GGCCTTTGCG CACACAAGTC CCTTCCTGGG CATCCTGCAT 
CCTGGCCAGT GCATACAGGC GATTGAGAAC AACATGTACC GAGCGCCTAT TTATCCACAT 
AAGATGGCTC ACAACGATTT CCTCGTCATT CGCACGCGGA ACAACTACTG GATACGGTCG 
GTGAATTCAA TATACACCGT GGGTCAGGAG TGTCCGCTGT ACGAGGTACC CGGTCCGAAT 
TCCAAAAGGG CCAACAACTT CACCCGTGAC TTTCTGCAGG TGTTTATTTA CCGCCTGTTC 
TGGAAAAGTC GCGACAACCC GCGCCGCATT CGAATGGACG ATATAAAACA GGCTTTTCCC 
GCTCATTCCG AGAGCAGCAT CCGCAAGCGT TTAAAGCAGT GCGCTGACTT CAAGCGAACA 
GGCATGGACT CCAATTGGTG GGTTATAAAG CCAGAGTTTC GCCTTCCATC CGAGGAGGAG 
ATCCGAGCCA TGGTGTCACC TGAGCAGTGT TGCGCGTACT TCAGCATGAT AGCGGCGGAA 
CAACGCTTAA AGGATGCTGG GTATGGAGAA AAGTTTTTGT TCGCACCTCA GGAAGATGAC 
GACGAGGAGG CGCAGCTGAA GCTTGACGAC GAAGTAAAGG TGGCTCCTTG GAACACGACT 
CGCGCATATA TCCAAGCCAT GCGGGGAAAG TGTTTACTCC AGTTGAGTGG TCCAGCCGAT 
CCAACGGGAT GTGGAGAGGG ATTTTCATAT GTTCGAGTGC CAAACAAGCC CACGCAAACC 
AAGGAGGAGC AAGAGTCGCA GCCTAAACGT TCGGTCACAG GAACAGATGC AGATTTGCGT 
CGTCTGCCAC TCCAGCGTGC AAAAGAGCTG TTGCGGCAGT TCAAGGTGCC CGAGGAGGAG 
ATCAAAAAGC TTTCCCGCTG GGAGGTCATT GACGTGGTGC GCACCCTGTC CACAGAAAAG 
GCCAAGGCCG GTGAAGAGGG AATGGATAAG TTTTCTCGTG GCAACCGGTT CTCCATTGCA 
GAGCATCAGG AGCGTTATAA GGAAGAGTGC CAGCGCATAT TCGATCTGCA AAACAGAGTG 
CTGGCCAGCT CTGAGGTGCT GTCCACAGAT GAGGCAGAGT CCTCGGCCTC TGAGGAATCT 
GATCTCGAAG AACTTGGCAA GAATCTTGAG AACATGCTGT CAAACAAGAA AACCTCGACG 
CAATTGTCAA GGGAACGTGA AGAGCTGGAG CGTCAGGAGT TGCTTCGCCA GCTTGACGAA 
GAACACGGCG GACCAAGTGG TAGTGGAGGA GCCAAGGGAG CCAAAGGAAA GGATGATCCG 
GGACAGCAAA TGCTGGCAAC CAACAACCAG GGCAGGATCC TTCGCATTAC GCGTACCTTT 
AGAGGTAACG ATGGCAAGGA ATATACTCGC GTGGAGACTG TGCGGCGGCA ACCAGTTATC 
GACGCCTACA TCAAGATTCG CACCACTAAG GACGAGCAGT TCATCAAGCA GTTCGCAACG 
CTAGATGAGC AGCAGAAGGA GGAGATGAAG CGCGAAAAGA GACGCATTCA GGAGCAGCTA 
CGTCGCATCA AGCGCAACCA GGAGCGCGAA CGCCTGGCGC AGCTGGCCCA GAACCAGAAG 
CTTCAGCCAG GTGGCATGCC CACTTCCTTG GGTGATCCTA AGAGCTCGGG CGGTCATTCG 
CACAAGGAGC GGGATAGCGG CTACAAGGAG GTCAGCCCTT CGCGCAAGAA GTTCAAGCTT 
AAGCCAGACC TAAAGCTGAA GTGCGGCGCC TGTGGACAGG TTGGTCACAT GCGCACAAAC 
AAAGCCTGTC CCTTGTATTC TGGCATGCAA AGCAGTCTGT CCCAGTCGAA CCCATCTCTG 
GCTGACGATT TTGACGAACA GAGCGAAAAG GAGATGACAA TGGATGACGA TGATCTTGTG 
AATGTCGATG GCACCAAAGT AACGCTCAGC AGTAAGATTC TCAAGCGTCA TGGTGGTGAT 
GATGGCAAGC GTCGCAGCGG ATCTAGCTCT GGTTTCACCT TGAAGGTTCC CCGAGATGCG 
ATGGGCAAGA AGAAACGCAG AGTGGGTGGC GATCTTCATT GTGACTATCT GCAGCGACAC 
AATAAAACGG CCAATCGCAG GCGCACGGAC CCCGTTGTGG TACTGTCCTC TATCCTGGAG 
ATTATCCATA ATGAGCTGCG ATCTATGCCA GATGTATCGC CATTCCTGTT CCCGGTAAGC 
GCAAAAAAGG TTCCCGACTA CTACCGCGTG GTGACCAAGC CCATGGATCT GCAAACGATG 
AGGGAGTATA TTCGCCAAAG GCGCTACACG AGTCGCGAGA TGTTCCTCGA GGATCTCAAG 
CAGATTGTGG ACAACTCGCT GATCTACAAT GGACCGCAGA GTGCATACAC CTTGGCTGCC 
CAACGCATGT TCAGCAGTTG TTTTGAATTG CTCGCAGAGC GCGAAGACAA ACTGATGCGC 
CTCGAGAAGG CAATTAACCC GCTGCTGGAC GACGATGACC AAGTGGCACT CTCCTTTATC 
TTTGACAAGC TGCACTCGCA GATTAAGCAA TTACCAGAGA GCTGGCCTTT CCTTAAGCCT 
GTCAACAAGA AACAGGTTAA GGACTACTAC ACGGTTATCA AGCGACCCAT GGACCTCGAA 
ACTATCGGCA AAAACATTGA AGCTCATCGC TATCACAGTC GTGCCGAGTA TCTGGCTGAT 
ATCGAGTTGA TCGCCACCAA CTGTGAGCAG TACAACGGCA GTGACACCCG CTACACCAAG 
TTCTCAAAGA AGATACTTGA GTATGCCCAA ACCCAGTTAA TTGAGTTTTC GGAGCACTGC 
GGCCAGTTGG AAAATAACAT AGCTAAGACG CAGGAGCGTG CTAGGGAAAA TGCACCAGAG 
TTTGATGAAG CCTGGGGCAA TGATGATTAC AACTTTGACC GTGGCAGTAG GGCCAGTTCA 
CCCGGAGATG ACTACATCGA CGTCGAGGGT CATGGGGGGC ATGCCTCCTC ATCGAACTCT 
ATCCATCGCA GCATGGGCGC CGAGGCCGGT TCGTCACATA CGGCGCCGGC GGTGCGAAAA 
CCAGCTCCTC CTGGTCCTGG TGAGGTGAAG CGCGGAAGGG GTAGGCCCCG CAAGCAGCGC 
GACCCCGTGG AGGAGGTCAA ATCCCAGAAT CCGGTTAAGC GTGGTCGGGG GCGTCCGAGG 
AAGGACAGCC TTGCCTCAAA CATGAGTCAC ACGCAAGCTT ACTTCCTGGA TGAAGATCTC 
CAATGCTCCA CAGATGACGA GGACGACGAC GAGGAGGAGG ACTTCCAGGA GGTCTCCGAA 
GACGAGAACA ATGCGGCGAG CATTTTAGAT CAGGGCGAAC GTATCAATGC GCCTGCCGAT 
GCCATGGATG GCATGTTTGA CCCCAAGAAC ATCAAGACAG AGATTGACCT AGAGGCTCAC 
CAGATGGCAG AGGAGCCGAT CGGCGAGGAT GACAGCCAGC AGGTGGCCGA AGCAATGGTG 
CAGTTGAGTG GCGTGGGCGG CTACTATGCT CAACAGCAGC AAGATGAATC CATGGATGTG 
GACCCCAACT ACGATCCCTC AGATTTCCTC GCCATGCACA AGCAGCGCCA GAGCCTCGGC 
GAGCCCAGCA GCTTGCAGGG TGCTTTCACC AACTTCCTAT CGCACGAGCA GGATGATAAT 
GGGCCTTACA ATCCCGCCGA AGCCAGCACA AGTGCCGCTT CCGGTGCAGA CTTAGGAATG 
GACGCTTCAA TGGCCATGCA AATGGCGCCG GAAATGCCTG TCAATACCAT GAACAACGGA 
ATGGGCATCG ATGATGATCT GGATATTTCG GAGAGTGACG AGGAAGACGA TGGTTCTCGA 
GTGCGTATCA AAAAGGAGGT CTTCGACGAC GGGGATTACG CCTTGCAGCA CCAGCAGATG 
GGACAGGCAG CATCGCAGTC GCAGATATAC ATGGTGGATT CGTCCAACGA GCCCACGACT 
CTCGACTACC AGCAACCACC GCAACTGGAC TTCCAACAAG TGCAGGAAAT GGAGCAGTTG 
CAGCACCAAG TGATGCCACC AATGCAATCA GAGCAACTGC AGCAGCAACA GACGCCGCAG 
GGAGACAATG ATTATGCCTG GACTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Taf1 ( NM_206437.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996159.1 Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant C, mRNA.

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CloneID	OFa04102
Clone ID Related Accession (Same CDS sequence)	NM_206437.2
Accession Version	NM_206437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TBP-associated factor 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_206437.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA CTAAGGACGA CAAGGAACTA ATGCCTCCAC CAAGTGCACC AATGCGCTCC 
GGCTCTGGCG GCGGCATCGA GGAACCGGCC AAGTCAAATG ATGCATCTAG TCCCAGTGAC 
GATTCCAAAT CTACCGATTC AAAGGATGCG GATCGGAAGC TGGACACACC ACTGGCAGAC 
ATACTGCCGT CCAAGTACCA GAATGTGGAT GTGCGTGAGC TCTTTCCGGA CTTTCGTCCC 
CAAAAGGTGC TACGCTTCTC CCGTCTCTTC GGACCCGGTA AACCCACGAG TTTGCCCCAG 
ATCTGGCGAC ACGTGCGCAA ACGCCGCCGC AAGCGAAATC AATCCAGGGA TCAGAAGACG 
ACAAACACTG GTGGTTCGGA CTCTCCAAGC GATACTGAGG AGCCACGAAA GCGGGGCTTC 
AGTCTGCACT ATGCTGCAGA GCCAACGCCA GCGGAGTGCA TGTCCGACGA CGAGGACAAA 
TTGCTGGGCG ACTTCAACAG CGAGGATGTG CGTCCAGAAG GACCGGACAA CGGCGAGAAC 
AGCGATCAGA AGCCAAAGGT GGCTGACTGG CGGTTCGGGC CTGCACAGAT TTGGTATGAT 
ATGCTAGAGG TGCCCGACTC TGGAGAGGGG TTCAACTATG GCTTCAAGAC AAAGGCAGCA 
AGCACCTCGT CTCAGCCGCA ACTCAAAGAT GAACGCCGTG TAAAGAGTCC AGAAGATGAT 
GTCGAAGATC CAAGCATTGC GGATGATGCC TTTCTCATGG TCTCCCAGCT GCATTGGGAA 
GACGACGTGG TCTGGGACGG TAACGACATC AAGGCAAAGG TGCTCCAAAA GCTTAACTCA 
AAGACAAATG CAGCCGGATG GTTGCCCTCG AGCGGGTCGA GAACGGCTGG CGCCTTTAGC 
CAGCCGGGCA AACCGTCTAT GCCCGTGGGA AGTGGAAGCT CCAAGCAGGG TTCGGGAGCA 
TCAAGCAAAA AGGCCCAGCA AAATGCTCAA GCAAAGCCAG CGGAGGCACC TGATGACACC 
TGGTATAGCC TTTTTCCAGT TGAAAATGAG GAGCTAATAT ACTACAAGTG GGAGGACGAG 
GTAATCTGGG ATGCCCAGCA AGTGAGCAAG GTGCCCAAGC CGAAGGTACT CACTTTGGAT 
CCCAACGACG AAAACATCAT TTTGGGTATT CCTGATGACA TAGATCCCTC TAAAATCAAT 
AAGAGCACGG GTCCTCCACC TAAGATCAAG ATACCGCATC CTCACGTAAA AAAGTCTAAG 
ATCCTACTTG GCAAGGCGGG TGTGATCAAC GTGCTTGCGG AGGACACACC TCCGCCGCCA 
CCCAAAAGTC CTGACCGTGA CCCCTTTAAC ATATCCAATG ACACTTACTA CACACCGAAG 
ACAGAACCCA CCCTACGACT TAAGGTGGGC GGTAATCTTA TTCAACACTC GACTCCGGTG 
GTAGAGCTGC GAGCTCCGTT TGTGCCTACG CACATGGGCC CGATGAAGCT CCGCGCCTTC 
CATCGCCCCC CCCTAAAGAA GTACTCCCAC GGGCCAATGG CCCAGTCTAT CCCTCATCCT 
GTCTTCCCAC TGCTGAAGAC CATCGCAAAG AAGGCGAAGC AGCGCGAAGT GGAGCGCATT 
GCCTCCGGTG GCGGAGACGT CTTCTTTATG CGCAACCCAG AGGATTTAAG CGGAAGGGAC 
GGAGACATCG TACTAGCCGA GTTCTGCGAA GAGCATCCGC CTCTGATAAA CCAGGTGGGC 
ATGTGCTCCA AGATAAAGAA CTACTACAAG CGTAAGGCTG AGAAGGACAG TGGGCCGCAG 
GATTTTGTTT ATGGAGAAGT GGCCTTTGCG CACACAAGTC CCTTCCTGGG CATCCTGCAT 
CCTGGCCAGT GCATACAGGC GATTGAGAAC AACATGTACC GAGCGCCTAT TTATCCACAT 
AAGATGGCTC ACAACGATTT CCTCGTCATT CGCACGCGGA ACAACTACTG GATACGGTCG 
GTGAATTCAA TATACACCGT GGGTCAGGAG TGTCCGCTGT ACGAGGTACC CGGTCCGAAT 
TCCAAAAGGG CCAACAACTT CACCCGTGAC TTTCTGCAGG TGTTTATTTA CCGCCTGTTC 
TGGAAAAGTC GCGACAACCC GCGCCGCATT CGAATGGACG ATATAAAACA GGCTTTTCCC 
GCTCATTCCG AGAGCAGCAT CCGCAAGCGT TTAAAGCAGT GCGCTGACTT CAAGCGAACA 
GGCATGGACT CCAATTGGTG GGTTATAAAG CCAGAGTTTC GCCTTCCATC CGAGGAGGAG 
ATCCGAGCCA TGGTGTCACC TGAGCAGTGT TGCGCGTACT TCAGCATGAT AGCGGCGGAA 
CAACGCTTAA AGGATGCTGG GTATGGAGAA AAGTTTTTGT TCGCACCTCA GGAAGATGAC 
GACGAGGAGG CGCAGCTGAA GCTTGACGAC GAAGTAAAGG TGGCTCCTTG GAACACGACT 
CGCGCATATA TCCAAGCCAT GCGGGGAAAG TGTTTACTCC AGTTGAGTGG TCCAGCCGAT 
CCAACGGGAT GTGGAGAGGG ATTTTCATAT GTTCGAGTGC CAAACAAGCC CACGCAAACC 
AAGGAGGAGC AAGAGTCGCA GCCTAAACGT TCGGTCACAG GAACAGATGC AGATTTGCGT 
CGTCTGCCAC TCCAGCGTGC AAAAGAGCTG TTGCGGCAGT TCAAGGTGCC CGAGGAGGAG 
ATCAAAAAGC TTTCCCGCTG GGAGGTCATT GACGTGGTGC GCACCCTGTC CACAGAAAAG 
GCCAAGGCCG GTGAAGAGGG AATGGATAAG TTTTCTCGTG GCAACCGGTT CTCCATTGCA 
GAGCATCAGG AGCGTTATAA GGAAGAGTGC CAGCGCATAT TCGATCTGCA AAACAGAGTG 
CTGGCCAGCT CTGAGGTGCT GTCCACAGAT GAGGCAGAGT CCTCGGCCTC TGAGGAATCT 
GATCTCGAAG AACTTGGCAA GAATCTTGAG AACATGCTGT CAAACAAGAA AACCTCGACG 
CAATTGTCAA GGGAACGTGA AGAGCTGGAG CGTCAGGAGT TGCTTCGCCA GCTTGACGAA 
GAACACGGCG GACCAAGTGG TAGTGGAGGA GCCAAGGGAG CCAAAGGAAA GGATGATCCG 
GGACAGCAAA TGCTGGCAAC CAACAACCAG GGCAGGATCC TTCGCATTAC GCGTACCTTT 
AGAGGTAACG ATGGCAAGGA ATATACTCGC GTGGAGACTG TGCGGCGGCA ACCAGTTATC 
GACGCCTACA TCAAGATTCG CACCACTAAG GACGAGCAGT TCATCAAGCA GTTCGCAACG 
CTAGATGAGC AGCAGAAGGA GGAGATGAAG CGCGAAAAGA GACGCATTCA GGAGCAGCTA 
CGTCGCATCA AGCGCAACCA GGAGCGCGAA CGCCTGGCGC AGCTGGCCCA GAACCAGAAG 
CTTCAGCCAG GTGGCATGCC CACTTCCTTG GGTGATCCTA AGAGCTCGGG CGGTCATTCG 
CACAAGGAGC GGGATAGCGG CTACAAGGAG GTCAGCCCTT CGCGCAAGAA GTTCAAGCTT 
AAGCCAGACC TAAAGCTGAA GTGCGGCGCC TGTGGACAGG TTGGTCACAT GCGCACAAAC 
AAAGCCTGTC CCTTGTATTC TGGCATGCAA AGCAGTCTGT CCCAGTCGAA CCCATCTCTG 
GCTGACGATT TTGACGAACA GAGCGAAAAG GAGATGACAA TGGATGACGA TGATCTTGTG 
AATGTCGATG GCACCAAAGT AACGCTCAGC AGTAAGATTC TCAAGCGTCA TGGTGGTGAT 
GATGGCAAGC GTCGCAGCGG ATCTAGCTCT GGTTTCACCT TGAAGGTTCC CCGAGATGCG 
ATGGGCAAGA AGAAACGCAG AGTGGGTGGC GATCTTCATT GTGACTATCT GCAGCGACAC 
AATAAAACGG CCAATCGCAG GCGCACGGAC CCCGTTGTGG TACTGTCCTC TATCCTGGAG 
ATTATCCATA ATGAGCTGCG ATCTATGCCA GATGTATCGC CATTCCTGTT CCCGGTAAGC 
GCAAAAAAGG TTCCCGACTA CTACCGCGTG GTGACCAAGC CCATGGATCT GCAAACGATG 
AGGGAGTATA TTCGCCAAAG GCGCTACACG AGTCGCGAGA TGTTCCTCGA GGATCTCAAG 
CAGATTGTGG ACAACTCGCT GATCTACAAT GGACCGCAGA GTGCATACAC CTTGGCTGCC 
CAACGCATGT TCAGCAGTTG TTTTGAATTG CTCGCAGAGC GCGAAGACAA ACTGATGCGC 
CTCGAGAAGG CAATTAACCC GCTGCTGGAC GACGATGACC AAGTGGCACT CTCCTTTATC 
TTTGACAAGC TGCACTCGCA GATTAAGCAA TTACCAGAGA GCTGGCCTTT CCTTAAGCCT 
GTCAACAAGA AACAGGTTAA GGACTACTAC ACGGTTATCA AGCGACCCAT GGACCTCGAA 
ACTATCGGCA AAAACATTGA AGCTCATCGC TATCACAGTC GTGCCGAGTA TCTGGCTGAT 
ATCGAGTTGA TCGCCACCAA CTGTGAGCAG TACAACGGCA GTGACACCCG CTACACCAAG 
TTCTCAAAGA AGATACTTGA GTATGCCCAA ACCCAGTTAA TTGAGTTTTC GGAGCACTGC 
GGCCAGTTGG AAAATAACAT AGCTAAGACG CAGGAGCGTG CTAGGGAAAA TGCACCAGAG 
TTTGATGAAG CCTGGGGCAA TGATGATTAC AACTTTGACC GTGGCAGTAG GGCCAGTTCA 
CCCGGAGATG ACTACATCGA CGTCGAGGGT CATGGGGGGC ATGCCTCCTC ATCGAACTCT 
ATCCATCGCA GCATGGGCGC CGAGGCCGGT TCGTCACATA CGGCGCCGGC GGTGCGAAAA 
CCAGCTCCTC CTGGTCCTGG TGAGGTGAAG CGCGGAAGGG GTAGGCCCCG CAAGCAGCGC 
GACCCCGTGG AGGAGGTCAA ATCCCAGAAT CCGGTTAAGC GTGGTCGGGG GCGTCCGAGG 
AAGGACAGCC TTGCCTCAAA CATGAGTCAC ACGCAAGCTT ACTTCCTGGA TGAAGATCTC 
CAATGCTCCA CAGATGACGA GGACGACGAC GAGGAGGAGG ACTTCCAGGA GGTCTCCGAA 
GACGAGAACA ATGCGGCGAG CATTTTAGAT CAGGGCGAAC GTATCAATGC GCCTGCCGAT 
GCCATGGATG GCATGTTTGA CCCCAAGAAC ATCAAGACAG AGATTGACCT AGAGGCTCAC 
CAGATGGCAG ATGAATCCAT GGATGTGGAC CCCAACTACG ATCCCTCAGA TTTCCTCGCC 
ATGCACAAGC AGCGCCAGAG CCTCGGCGAG CCCAGCAGCT TGCAGGGTGC TTTCACCAAC 
TTCCTATCGC ACGAGCAGGA TGATAATGGG CCTTACAATC CCGCCGAAGC CAGCACAAGT 
GCCGCTTCCG GTGCAGACTT AGGAATGGAC GCTTCAATGG CCATGCAAAT GGCGCCGGAA 
ATGCCTGTCA ATACCATGAA CAACGGAATG GGCATCGATG ATGATCTGGA TATTTCGGAG 
AGTGACGAGG AAGACGATGG TTCTCGAGTG CGTATCAAAA AGGAGGTCTT CGACGACGGG 
GATTACGCCT TGCAGCACCA GCAGATGGGA CAGGCAGCAT CGCAGTCGCA GATATACATG 
GTGGATTCGT CCAACGAGCC CACGACTCTC GACTACCAGC AACCACCGCA ACTGGACTTC 
CAACAAGTGC AGGAAATGGA GCAGTTGCAG CACCAAGTGA TGCCACCAAT GCAATCAGAG 
CAACTGCAGC AGCAACAGAC GCCGCAGGGA GACAATGATT ATGCCTGGAC TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996159.1
CDS	102..6398
Translation	MEMESDNSDDEGSIGNGLDLTGILFGNIDSEGRLLQDDDGEGRGGTGFDAELRENIGSLSKLGLDSMLLEVIDLKEAEPPSDDEEEEDARPSAVSASEGMSAFDALKAGVKREDGAVKAQDDAIDYSDITELSEDCPRTPPEETSTYDDLEDAIPASKVEAKLTKDDKELMPPPSAPMRSGSGGGIEEPAKSNDASSPSDDSKSTDSKDADRKLDTPLADILPSKYQNVDVRELFPDFRPQKVLRFSRLFGPGKPTSLPQIWRHVRKRRRKRNQSRDQKTTNTGGSDSPSDTEEPRKRGFSLHYAAEPTPAECMSDDEDKLLGDFNSEDVRPEGPDNGENSDQKPKVADWRFGPAQIWYDMLEVPDSGEGFNYGFKTKAASTSSQPQLKDERRVKSPEDDVEDPSIADDAFLMVSQLHWEDDVVWDGNDIKAKVLQKLNSKTNAAGWLPSSGSRTAGAFSQPGKPSMPVGSGSSKQGSGASSKKAQQNAQAKPAEAPDDTWYSLFPVENEELIYYKWEDEVIWDAQQVSKVPKPKVLTLDPNDENIILGIPDDIDPSKINKSTGPPPKIKIPHPHVKKSKILLGKAGVINVLAEDTPPPPPKSPDRDPFNISNDTYYTPKTEPTLRLKVGGNLIQHSTPVVELRAPFVPTHMGPMKLRAFHRPPLKKYSHGPMAQSIPHPVFPLLKTIAKKAKQREVERIASGGGDVFFMRNPEDLSGRDGDIVLAEFCEEHPPLINQVGMCSKIKNYYKRKAEKDSGPQDFVYGEVAFAHTSPFLGILHPGQCIQAIENNMYRAPIYPHKMAHNDFLVIRTRNNYWIRSVNSIYTVGQECPLYEVPGPNSKRANNFTRDFLQVFIYRLFWKSRDNPRRIRMDDIKQAFPAHSESSIRKRLKQCADFKRTGMDSNWWVIKPEFRLPSEEEIRAMVSPEQCCAYFSMIAAEQRLKDAGYGEKFLFAPQEDDDEEAQLKLDDEVKVAPWNTTRAYIQAMRGKCLLQLSGPADPTGCGEGFSYVRVPNKPTQTKEEQESQPKRSVTGTDADLRRLPLQRAKELLRQFKVPEEEIKKLSRWEVIDVVRTLSTEKAKAGEEGMDKFSRGNRFSIAEHQERYKEECQRIFDLQNRVLASSEVLSTDEAESSASEESDLEELGKNLENMLSNKKTSTQLSREREELERQELLRQLDEEHGGPSGSGGAKGAKGKDDPGQQMLATNNQGRILRITRTFRGNDGKEYTRVETVRRQPVIDAYIKIRTTKDEQFIKQFATLDEQQKEEMKREKRRIQEQLRRIKRNQERERLAQLAQNQKLQPGGMPTSLGDPKSSGGHSHKERDSGYKEVSPSRKKFKLKPDLKLKCGACGQVGHMRTNKACPLYSGMQSSLSQSNPSLADDFDEQSEKEMTMDDDDLVNVDGTKVTLSSKILKRHGGDDGKRRSGSSSGFTLKVPRDAMGKKKRRVGGDLHCDYLQRHNKTANRRRTDPVVVLSSILEIIHNELRSMPDVSPFLFPVSAKKVPDYYRVVTKPMDLQTMREYIRQRRYTSREMFLEDLKQIVDNSLIYNGPQSAYTLAAQRMFSSCFELLAEREDKLMRLEKAINPLLDDDDQVALSFIFDKLHSQIKQLPESWPFLKPVNKKQVKDYYTVIKRPMDLETIGKNIEAHRYHSRAEYLADIELIATNCEQYNGSDTRYTKFSKKILEYAQTQLIEFSEHCGQLENNIAKTQERARENAPEFDEAWGNDDYNFDRGSRASSPGDDYIDVEGHGGHASSSNSIHRSMGAEAGSSHTAPAVRKPAPPGPGEVKRGRGRPRKQRDPVEEVKSQNPVKRGRGRPRKDSLASNMSHTQAYFLDEDLQCSTDDEDDDEEEDFQEVSEDENNAASILDQGERINAPADAMDGMFDPKNIKTEIDLEAHQMADESMDVDPNYDPSDFLAMHKQRQSLGEPSSLQGAFTNFLSHEQDDNGPYNPAEASTSAASGADLGMDASMAMQMAPEMPVNTMNNGMGIDDDLDISESDEEDDGSRVRIKKEVFDDGDYALQHQQMGQAASQSQIYMVDSSNEPTTLDYQQPPQLDFQQVQEMEQLQHQVMPPMQSEQLQQQQTPQGDNDYAWTF

Target ORF information:

RefSeq Version	NM_206437.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206437.2

ORF Insert Sequence:

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA CTAAGGACGA CAAGGAACTA ATGCCTCCAC CAAGTGCACC AATGCGCTCC 
GGCTCTGGCG GCGGCATCGA GGAACCGGCC AAGTCAAATG ATGCATCTAG TCCCAGTGAC 
GATTCCAAAT CTACCGATTC AAAGGATGCG GATCGGAAGC TGGACACACC ACTGGCAGAC 
ATACTGCCGT CCAAGTACCA GAATGTGGAT GTGCGTGAGC TCTTTCCGGA CTTTCGTCCC 
CAAAAGGTGC TACGCTTCTC CCGTCTCTTC GGACCCGGTA AACCCACGAG TTTGCCCCAG 
ATCTGGCGAC ACGTGCGCAA ACGCCGCCGC AAGCGAAATC AATCCAGGGA TCAGAAGACG 
ACAAACACTG GTGGTTCGGA CTCTCCAAGC GATACTGAGG AGCCACGAAA GCGGGGCTTC 
AGTCTGCACT ATGCTGCAGA GCCAACGCCA GCGGAGTGCA TGTCCGACGA CGAGGACAAA 
TTGCTGGGCG ACTTCAACAG CGAGGATGTG CGTCCAGAAG GACCGGACAA CGGCGAGAAC 
AGCGATCAGA AGCCAAAGGT GGCTGACTGG CGGTTCGGGC CTGCACAGAT TTGGTATGAT 
ATGCTAGAGG TGCCCGACTC TGGAGAGGGG TTCAACTATG GCTTCAAGAC AAAGGCAGCA 
AGCACCTCGT CTCAGCCGCA ACTCAAAGAT GAACGCCGTG TAAAGAGTCC AGAAGATGAT 
GTCGAAGATC CAAGCATTGC GGATGATGCC TTTCTCATGG TCTCCCAGCT GCATTGGGAA 
GACGACGTGG TCTGGGACGG TAACGACATC AAGGCAAAGG TGCTCCAAAA GCTTAACTCA 
AAGACAAATG CAGCCGGATG GTTGCCCTCG AGCGGGTCGA GAACGGCTGG CGCCTTTAGC 
CAGCCGGGCA AACCGTCTAT GCCCGTGGGA AGTGGAAGCT CCAAGCAGGG TTCGGGAGCA 
TCAAGCAAAA AGGCCCAGCA AAATGCTCAA GCAAAGCCAG CGGAGGCACC TGATGACACC 
TGGTATAGCC TTTTTCCAGT TGAAAATGAG GAGCTAATAT ACTACAAGTG GGAGGACGAG 
GTAATCTGGG ATGCCCAGCA AGTGAGCAAG GTGCCCAAGC CGAAGGTACT CACTTTGGAT 
CCCAACGACG AAAACATCAT TTTGGGTATT CCTGATGACA TAGATCCCTC TAAAATCAAT 
AAGAGCACGG GTCCTCCACC TAAGATCAAG ATACCGCATC CTCACGTAAA AAAGTCTAAG 
ATCCTACTTG GCAAGGCGGG TGTGATCAAC GTGCTTGCGG AGGACACACC TCCGCCGCCA 
CCCAAAAGTC CTGACCGTGA CCCCTTTAAC ATATCCAATG ACACTTACTA CACACCGAAG 
ACAGAACCCA CCCTACGACT TAAGGTGGGC GGTAATCTTA TTCAACACTC GACTCCGGTG 
GTAGAGCTGC GAGCTCCGTT TGTGCCTACG CACATGGGCC CGATGAAGCT CCGCGCCTTC 
CATCGCCCCC CCCTAAAGAA GTACTCCCAC GGGCCAATGG CCCAGTCTAT CCCTCATCCT 
GTCTTCCCAC TGCTGAAGAC CATCGCAAAG AAGGCGAAGC AGCGCGAAGT GGAGCGCATT 
GCCTCCGGTG GCGGAGACGT CTTCTTTATG CGCAACCCAG AGGATTTAAG CGGAAGGGAC 
GGAGACATCG TACTAGCCGA GTTCTGCGAA GAGCATCCGC CTCTGATAAA CCAGGTGGGC 
ATGTGCTCCA AGATAAAGAA CTACTACAAG CGTAAGGCTG AGAAGGACAG TGGGCCGCAG 
GATTTTGTTT ATGGAGAAGT GGCCTTTGCG CACACAAGTC CCTTCCTGGG CATCCTGCAT 
CCTGGCCAGT GCATACAGGC GATTGAGAAC AACATGTACC GAGCGCCTAT TTATCCACAT 
AAGATGGCTC ACAACGATTT CCTCGTCATT CGCACGCGGA ACAACTACTG GATACGGTCG 
GTGAATTCAA TATACACCGT GGGTCAGGAG TGTCCGCTGT ACGAGGTACC CGGTCCGAAT 
TCCAAAAGGG CCAACAACTT CACCCGTGAC TTTCTGCAGG TGTTTATTTA CCGCCTGTTC 
TGGAAAAGTC GCGACAACCC GCGCCGCATT CGAATGGACG ATATAAAACA GGCTTTTCCC 
GCTCATTCCG AGAGCAGCAT CCGCAAGCGT TTAAAGCAGT GCGCTGACTT CAAGCGAACA 
GGCATGGACT CCAATTGGTG GGTTATAAAG CCAGAGTTTC GCCTTCCATC CGAGGAGGAG 
ATCCGAGCCA TGGTGTCACC TGAGCAGTGT TGCGCGTACT TCAGCATGAT AGCGGCGGAA 
CAACGCTTAA AGGATGCTGG GTATGGAGAA AAGTTTTTGT TCGCACCTCA GGAAGATGAC 
GACGAGGAGG CGCAGCTGAA GCTTGACGAC GAAGTAAAGG TGGCTCCTTG GAACACGACT 
CGCGCATATA TCCAAGCCAT GCGGGGAAAG TGTTTACTCC AGTTGAGTGG TCCAGCCGAT 
CCAACGGGAT GTGGAGAGGG ATTTTCATAT GTTCGAGTGC CAAACAAGCC CACGCAAACC 
AAGGAGGAGC AAGAGTCGCA GCCTAAACGT TCGGTCACAG GAACAGATGC AGATTTGCGT 
CGTCTGCCAC TCCAGCGTGC AAAAGAGCTG TTGCGGCAGT TCAAGGTGCC CGAGGAGGAG 
ATCAAAAAGC TTTCCCGCTG GGAGGTCATT GACGTGGTGC GCACCCTGTC CACAGAAAAG 
GCCAAGGCCG GTGAAGAGGG AATGGATAAG TTTTCTCGTG GCAACCGGTT CTCCATTGCA 
GAGCATCAGG AGCGTTATAA GGAAGAGTGC CAGCGCATAT TCGATCTGCA AAACAGAGTG 
CTGGCCAGCT CTGAGGTGCT GTCCACAGAT GAGGCAGAGT CCTCGGCCTC TGAGGAATCT 
GATCTCGAAG AACTTGGCAA GAATCTTGAG AACATGCTGT CAAACAAGAA AACCTCGACG 
CAATTGTCAA GGGAACGTGA AGAGCTGGAG CGTCAGGAGT TGCTTCGCCA GCTTGACGAA 
GAACACGGCG GACCAAGTGG TAGTGGAGGA GCCAAGGGAG CCAAAGGAAA GGATGATCCG 
GGACAGCAAA TGCTGGCAAC CAACAACCAG GGCAGGATCC TTCGCATTAC GCGTACCTTT 
AGAGGTAACG ATGGCAAGGA ATATACTCGC GTGGAGACTG TGCGGCGGCA ACCAGTTATC 
GACGCCTACA TCAAGATTCG CACCACTAAG GACGAGCAGT TCATCAAGCA GTTCGCAACG 
CTAGATGAGC AGCAGAAGGA GGAGATGAAG CGCGAAAAGA GACGCATTCA GGAGCAGCTA 
CGTCGCATCA AGCGCAACCA GGAGCGCGAA CGCCTGGCGC AGCTGGCCCA GAACCAGAAG 
CTTCAGCCAG GTGGCATGCC CACTTCCTTG GGTGATCCTA AGAGCTCGGG CGGTCATTCG 
CACAAGGAGC GGGATAGCGG CTACAAGGAG GTCAGCCCTT CGCGCAAGAA GTTCAAGCTT 
AAGCCAGACC TAAAGCTGAA GTGCGGCGCC TGTGGACAGG TTGGTCACAT GCGCACAAAC 
AAAGCCTGTC CCTTGTATTC TGGCATGCAA AGCAGTCTGT CCCAGTCGAA CCCATCTCTG 
GCTGACGATT TTGACGAACA GAGCGAAAAG GAGATGACAA TGGATGACGA TGATCTTGTG 
AATGTCGATG GCACCAAAGT AACGCTCAGC AGTAAGATTC TCAAGCGTCA TGGTGGTGAT 
GATGGCAAGC GTCGCAGCGG ATCTAGCTCT GGTTTCACCT TGAAGGTTCC CCGAGATGCG 
ATGGGCAAGA AGAAACGCAG AGTGGGTGGC GATCTTCATT GTGACTATCT GCAGCGACAC 
AATAAAACGG CCAATCGCAG GCGCACGGAC CCCGTTGTGG TACTGTCCTC TATCCTGGAG 
ATTATCCATA ATGAGCTGCG ATCTATGCCA GATGTATCGC CATTCCTGTT CCCGGTAAGC 
GCAAAAAAGG TTCCCGACTA CTACCGCGTG GTGACCAAGC CCATGGATCT GCAAACGATG 
AGGGAGTATA TTCGCCAAAG GCGCTACACG AGTCGCGAGA TGTTCCTCGA GGATCTCAAG 
CAGATTGTGG ACAACTCGCT GATCTACAAT GGACCGCAGA GTGCATACAC CTTGGCTGCC 
CAACGCATGT TCAGCAGTTG TTTTGAATTG CTCGCAGAGC GCGAAGACAA ACTGATGCGC 
CTCGAGAAGG CAATTAACCC GCTGCTGGAC GACGATGACC AAGTGGCACT CTCCTTTATC 
TTTGACAAGC TGCACTCGCA GATTAAGCAA TTACCAGAGA GCTGGCCTTT CCTTAAGCCT 
GTCAACAAGA AACAGGTTAA GGACTACTAC ACGGTTATCA AGCGACCCAT GGACCTCGAA 
ACTATCGGCA AAAACATTGA AGCTCATCGC TATCACAGTC GTGCCGAGTA TCTGGCTGAT 
ATCGAGTTGA TCGCCACCAA CTGTGAGCAG TACAACGGCA GTGACACCCG CTACACCAAG 
TTCTCAAAGA AGATACTTGA GTATGCCCAA ACCCAGTTAA TTGAGTTTTC GGAGCACTGC 
GGCCAGTTGG AAAATAACAT AGCTAAGACG CAGGAGCGTG CTAGGGAAAA TGCACCAGAG 
TTTGATGAAG CCTGGGGCAA TGATGATTAC AACTTTGACC GTGGCAGTAG GGCCAGTTCA 
CCCGGAGATG ACTACATCGA CGTCGAGGGT CATGGGGGGC ATGCCTCCTC ATCGAACTCT 
ATCCATCGCA GCATGGGCGC CGAGGCCGGT TCGTCACATA CGGCGCCGGC GGTGCGAAAA 
CCAGCTCCTC CTGGTCCTGG TGAGGTGAAG CGCGGAAGGG GTAGGCCCCG CAAGCAGCGC 
GACCCCGTGG AGGAGGTCAA ATCCCAGAAT CCGGTTAAGC GTGGTCGGGG GCGTCCGAGG 
AAGGACAGCC TTGCCTCAAA CATGAGTCAC ACGCAAGCTT ACTTCCTGGA TGAAGATCTC 
CAATGCTCCA CAGATGACGA GGACGACGAC GAGGAGGAGG ACTTCCAGGA GGTCTCCGAA 
GACGAGAACA ATGCGGCGAG CATTTTAGAT CAGGGCGAAC GTATCAATGC GCCTGCCGAT 
GCCATGGATG GCATGTTTGA CCCCAAGAAC ATCAAGACAG AGATTGACCT AGAGGCTCAC 
CAGATGGCAG ATGAATCCAT GGATGTGGAC CCCAACTACG ATCCCTCAGA TTTCCTCGCC 
ATGCACAAGC AGCGCCAGAG CCTCGGCGAG CCCAGCAGCT TGCAGGGTGC TTTCACCAAC 
TTCCTATCGC ACGAGCAGGA TGATAATGGG CCTTACAATC CCGCCGAAGC CAGCACAAGT 
GCCGCTTCCG GTGCAGACTT AGGAATGGAC GCTTCAATGG CCATGCAAAT GGCGCCGGAA 
ATGCCTGTCA ATACCATGAA CAACGGAATG GGCATCGATG ATGATCTGGA TATTTCGGAG 
AGTGACGAGG AAGACGATGG TTCTCGAGTG CGTATCAAAA AGGAGGTCTT CGACGACGGG 
GATTACGCCT TGCAGCACCA GCAGATGGGA CAGGCAGCAT CGCAGTCGCA GATATACATG 
GTGGATTCGT CCAACGAGCC CACGACTCTC GACTACCAGC AACCACCGCA ACTGGACTTC 
CAACAAGTGC AGGAAATGGA GCAGTTGCAG CACCAAGTGA TGCCACCAAT GCAATCAGAG 
CAACTGCAGC AGCAACAGAC GCCGCAGGGA GACAATGATT ATGCCTGGAC TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Taf1 ( NM_057608.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476956.3 Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant A, mRNA.

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CloneID	OFa04103
Clone ID Related Accession (Same CDS sequence)	NM_057608.5
Accession Version	NM_057608.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TBP-associated factor 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_057608.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA CTAAGGACGA CAAGGAACTA ATGCCTCCAC CAAGTGCACC AATGCGCTCC 
GGCTCTGGCG GCGGCATCGA GGAACCGGCC AAGTCAAATG ATGCATCTAG TCCCAGTGAC 
GATTCCAAAT CTACCGATTC AAAGGATGCG GATCGGAAGC TGGACACACC ACTGGCAGAC 
ATACTGCCGT CCAAGTACCA GAATGTGGAT GTGCGTGAGC TCTTTCCGGA CTTTCGTCCC 
CAAAAGGTGC TACGCTTCTC CCGTCTCTTC GGACCCGGTA AACCCACGAG TTTGCCCCAG 
ATCTGGCGAC ACGTGCGCAA ACGCCGCCGC AAGCGAAATC AATCCAGGGA TCAGAAGACG 
ACAAACACTG GTGGTTCGGA CTCTCCAAGC GATACTGAGG AGCCACGAAA GCGGGGCTTC 
AGTCTGCACT ATGCTGCAGA GCCAACGCCA GCGGAGTGCA TGTCCGACGA CGAGGACAAA 
TTGCTGGGCG ACTTCAACAG CGAGGATGTG CGTCCAGAAG GACCGGACAA CGGCGAGAAC 
AGCGATCAGA AGCCAAAGGT GGCTGACTGG CGGTTCGGGC CTGCACAGAT TTGGTATGAT 
ATGCTAGAGG TGCCCGACTC TGGAGAGGGG TTCAACTATG GCTTCAAGAC AAAGGCAGCA 
AGCACCTCGT CTCAGCCGCA ACTCAAAGAT GAACGCCGTG TAAAGAGTCC AGAAGATGAT 
GTCGAAGATC CAAGCATTGC GGATGATGCC TTTCTCATGG TCTCCCAGCT GCATTGGGAA 
GACGACGTGG TCTGGGACGG TAACGACATC AAGGCAAAGG TGCTCCAAAA GCTTAACTCA 
AAGACAAATG CAGCCGGATG GTTGCCCTCG AGCGGGTCGA GAACGGCTGG CGCCTTTAGC 
CAGCCGGGCA AACCGTCTAT GCCCGTGGGA AGTGGAAGCT CCAAGCAGGG TTCGGGAGCA 
TCAAGCAAAA AGGCCCAGCA AAATGCTCAA GCAAAGCCAG CGGAGGCACC TGATGACACC 
TGGTATAGCC TTTTTCCAGT TGAAAATGAG GAGCTAATAT ACTACAAGTG GGAGGACGAG 
GTAATCTGGG ATGCCCAGCA AGTGAGCAAG GTGCCCAAGC CGAAGGTACT CACTTTGGAT 
CCCAACGACG AAAACATCAT TTTGGGTATT CCTGATGACA TAGATCCCTC TAAAATCAAT 
AAGAGCACGG GTCCTCCACC TAAGATCAAG ATACCGCATC CTCACGTAAA AAAGTCTAAG 
ATCCTACTTG GCAAGGCGGG TGTGATCAAC GTGCTTGCGG AGGACACACC TCCGCCGCCA 
CCCAAAAGTC CTGACCGTGA CCCCTTTAAC ATATCCAATG ACACTTACTA CACACCGAAG 
ACAGAACCCA CCCTACGACT TAAGGTGGGC GGTAATCTTA TTCAACACTC GACTCCGGTG 
GTAGAGCTGC GAGCTCCGTT TGTGCCTACG CACATGGGCC CGATGAAGCT CCGCGCCTTC 
CATCGCCCCC CCCTAAAGAA GTACTCCCAC GGGCCAATGG CCCAGTCTAT CCCTCATCCT 
GTCTTCCCAC TGCTGAAGAC CATCGCAAAG AAGGCGAAGC AGCGCGAAGT GGAGCGCATT 
GCCTCCGGTG GCGGAGACGT CTTCTTTATG CGCAACCCAG AGGATTTAAG CGGAAGGGAC 
GGAGACATCG TACTAGCCGA GTTCTGCGAA GAGCATCCGC CTCTGATAAA CCAGGTGGGC 
ATGTGCTCCA AGATAAAGAA CTACTACAAG CGTAAGGCTG AGAAGGACAG TGGGCCGCAG 
GATTTTGTTT ATGGAGAAGT GGCCTTTGCG CACACAAGTC CCTTCCTGGG CATCCTGCAT 
CCTGGCCAGT GCATACAGGC GATTGAGAAC AACATGTACC GAGCGCCTAT TTATCCACAT 
AAGATGGCTC ACAACGATTT CCTCGTCATT CGCACGCGGA ACAACTACTG GATACGGTCG 
GTGAATTCAA TATACACCGT GGGTCAGGAG TGTCCGCTGT ACGAGGTACC CGGTCCGAAT 
TCCAAAAGGG CCAACAACTT CACCCGTGAC TTTCTGCAGG TGTTTATTTA CCGCCTGTTC 
TGGAAAAGTC GCGACAACCC GCGCCGCATT CGAATGGACG ATATAAAACA GGCTTTTCCC 
GCTCATTCCG AGAGCAGCAT CCGCAAGCGT TTAAAGCAGT GCGCTGACTT CAAGCGAACA 
GGCATGGACT CCAATTGGTG GGTTATAAAG CCAGAGTTTC GCCTTCCATC CGAGGAGGAG 
ATCCGAGCCA TGGTGTCACC TGAGCAGTGT TGCGCGTACT TCAGCATGAT AGCGGCGGAA 
CAACGCTTAA AGGATGCTGG GTATGGAGAA AAGTTTTTGT TCGCACCTCA GGAAGATGAC 
GACGAGGAGG CGCAGCTGAA GCTTGACGAC GAAGTAAAGG TGGCTCCTTG GAACACGACT 
CGCGCATATA TCCAAGCCAT GCGGGGAAAG TGTTTACTCC AGTTGAGTGG TCCAGCCGAT 
CCAACGGGAT GTGGAGAGGG ATTTTCATAT GTTCGAGTGC CAAACAAGCC CACGCAAACC 
AAGGAGGAGC AAGAGTCGCA GCCTAAACGT TCGGTCACAG GAACAGATGC AGATTTGCGT 
CGTCTGCCAC TCCAGCGTGC AAAAGAGCTG TTGCGGCAGT TCAAGGTGCC CGAGGAGGAG 
ATCAAAAAGC TTTCCCGCTG GGAGGTCATT GACGTGGTGC GCACCCTGTC CACAGAAAAG 
GCCAAGGCCG GTGAAGAGGG AATGGATAAG TTTTCTCGTG GCAACCGGTT CTCCATTGCA 
GAGCATCAGG AGCGTTATAA GGAAGAGTGC CAGCGCATAT TCGATCTGCA AAACAGAGTG 
CTGGCCAGCT CTGAGGTGCT GTCCACAGAT GAGGCAGAGT CCTCGGCCTC TGAGGAATCT 
GATCTCGAAG AACTTGGCAA GAATCTTGAG AACATGCTGT CAAACAAGAA AACCTCGACG 
CAATTGTCAA GGGAACGTGA AGAGCTGGAG CGTCAGGAGT TGCTTCGCCA GCTTGACGAA 
GAACACGGCG GACCAAGTGG TAGTGGAGGA GCCAAGGGAG CCAAAGGAAA GGATGATCCG 
GGACAGCAAA TGCTGGCAAC CAACAACCAG GGCAGGATCC TTCGCATTAC GCGTACCTTT 
AGAGGTAACG ATGGCAAGGA ATATACTCGC GTGGAGACTG TGCGGCGGCA ACCAGTTATC 
GACGCCTACA TCAAGATTCG CACCACTAAG GACGAGCAGT TCATCAAGCA GTTCGCAACG 
CTAGATGAGC AGCAGAAGGA GGAGATGAAG CGCGAAAAGA GACGCATTCA GGAGCAGCTA 
CGTCGCATCA AGCGCAACCA GGAGCGCGAA CGCCTGGCGC AGCTGGCCCA GAACCAGAAG 
CTTCAGCCAG GTGGCATGCC CACTTCCTTG GGTGATCCTA AGAGCTCGGG CGGTCATTCG 
CACAAGGAGC GGGATAGCGG CTACAAGGAG GTCAGCCCTT CGCGCAAGAA GTTCAAGCTT 
AAGCCAGACC TAAAGCTGAA GTGCGGCGCC TGTGGACAGG TTGGTCACAT GCGCACAAAC 
AAAGCCTGTC CCTTGTATTC TGGCATGCAA AGCAGTCTGT CCCAGTCGAA CCCATCTCTG 
GCTGACGATT TTGACGAACA GAGCGAAAAG GAGATGACAA TGGATGACGA TGATCTTGTG 
AATGTCGATG GCACCAAAGT AACGCTCAGC AGTAAGATTC TCAAGCGTCA TGGTGGTGAT 
GATGGCAAGC GTCGCAGCGG ATCTAGCTCT GGTTTCACCT TGAAGGTTCC CCGAGATGCG 
ATGGGCAAGA AGAAACGCAG AGTGGGTGGC GATCTTCATT GTGACTATCT GCAGCGACAC 
AATAAAACGG CCAATCGCAG GCGCACGGAC CCCGTTGTGG TACTGTCCTC TATCCTGGAG 
ATTATCCATA ATGAGCTGCG ATCTATGCCA GATGTATCGC CATTCCTGTT CCCGGTAAGC 
GCAAAAAAGG TTCCCGACTA CTACCGCGTG GTGACCAAGC CCATGGATCT GCAAACGATG 
AGGGAGTATA TTCGCCAAAG GCGCTACACG AGTCGCGAGA TGTTCCTCGA GGATCTCAAG 
CAGATTGTGG ACAACTCGCT GATCTACAAT GGACCGCAGA GTGCATACAC CTTGGCTGCC 
CAACGCATGT TCAGCAGTTG TTTTGAATTG CTCGCAGAGC GCGAAGACAA ACTGATGCGC 
CTCGAGAAGG CAATTAACCC GCTGCTGGAC GACGATGACC AAGTGGCACT CTCCTTTATC 
TTTGACAAGC TGCACTCGCA GATTAAGCAA TTACCAGAGA GCTGGCCTTT CCTTAAGCCT 
GTCAACAAGA AACAGGTTAA GGACTACTAC ACGGTTATCA AGCGACCCAT GGACCTCGAA 
ACTATCGGCA AAAACATTGA AGCTCATCGC TATCACAGTC GTGCCGAGTA TCTGGCTGAT 
ATCGAGTTGA TCGCCACCAA CTGTGAGCAG TACAACGGCA GTGACACCCG CTACACCAAG 
TTCTCAAAGA AGATACTTGA GTATGCCCAA ACCCAGTTAA TTGAGTTTTC GGAGCACTGC 
GGCCAGTTGG AAAATAACAT AGCTAAGACG CAGGAGCGTG CTAGGGAAAA TGCACCAGAG 
TTTGATGAAG CCTGGGGCAA TGATGATTAC AACTTTGACC GTGGCAGTAG GGCCAGTTCA 
CCCGGAGATG ACTACATCGA CGTCGAGGGT CATGGGGGGC ATGCCTCCTC ATCGAACTCT 
ATCCATCGCA GCATGGGCGC CGAGGCCGGT TCGTCACATA CGGCGCCGGC GGTGCGAAAA 
CCAGCTCCTC CTGGTCCTGG TGAGGTGAAG CGCGGAAGGG GTAGGCCCCG CAAGCAGCGC 
GACCCCGTGG AGGAGGATCT CCAATGCTCC ACAGATGACG AGGACGACGA CGAGGAGGAG 
GACTTCCAGG AGGTCTCCGA AGACGAGAAC AATGCGGCGA GCATTTTAGA TCAGGGCGAA 
CGTATCAATG CGCCTGCCGA TGCCATGGAT GGCATGTTTG ACCCCAAGAA CATCAAGACA 
GAGATTGACC TAGAGGCTCA CCAGATGGCA GATGAATCCA TGGATGTGGA CCCCAACTAC 
GATCCCTCAG ATTTCCTCGC CATGCACAAG CAGCGCCAGA GCCTCGGCGA GCCCAGCAGC 
TTGCAGGGTG CTTTCACCAA CTTCCTATCG CACGAGCAGG ATGATAATGG GCCTTACAAT 
CCCGCCGAAG CCAGCACAAG TGCCGCTTCC GGTGCAGACT TAGGAATGGA CGCTTCAATG 
GCCATGCAAA TGGCGCCGGA AATGCCTGTC AATACCATGA ACAACGGAAT GGGCATCGAT 
GATGATCTGG ATATTTCGGA GAGTGACGAG GAAGACGATG GTTCTCGAGT GCGTATCAAA 
AAGGAGGTCT TCGACGACGG GGATTACGCC TTGCAGCACC AGCAGATGGG ACAGGCAGCA 
TCGCAGTCGC AGATATACAT GGTGGATTCG TCCAACGAGC CCACGACTCT CGACTACCAG 
CAACCACCGC AACTGGACTT CCAACAAGTG CAGGAAATGG AGCAGTTGCA GCACCAAGTG 
ATGCCACCAA TGCAATCAGA GCAACTGCAG CAGCAACAGA CGCCGCAGGG AGACAATGAT 
TATGCCTGGA CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476956.3
CDS	102..6299
Translation	MEMESDNSDDEGSIGNGLDLTGILFGNIDSEGRLLQDDDGEGRGGTGFDAELRENIGSLSKLGLDSMLLEVIDLKEAEPPSDDEEEEDARPSAVSASEGMSAFDALKAGVKREDGAVKAQDDAIDYSDITELSEDCPRTPPEETSTYDDLEDAIPASKVEAKLTKDDKELMPPPSAPMRSGSGGGIEEPAKSNDASSPSDDSKSTDSKDADRKLDTPLADILPSKYQNVDVRELFPDFRPQKVLRFSRLFGPGKPTSLPQIWRHVRKRRRKRNQSRDQKTTNTGGSDSPSDTEEPRKRGFSLHYAAEPTPAECMSDDEDKLLGDFNSEDVRPEGPDNGENSDQKPKVADWRFGPAQIWYDMLEVPDSGEGFNYGFKTKAASTSSQPQLKDERRVKSPEDDVEDPSIADDAFLMVSQLHWEDDVVWDGNDIKAKVLQKLNSKTNAAGWLPSSGSRTAGAFSQPGKPSMPVGSGSSKQGSGASSKKAQQNAQAKPAEAPDDTWYSLFPVENEELIYYKWEDEVIWDAQQVSKVPKPKVLTLDPNDENIILGIPDDIDPSKINKSTGPPPKIKIPHPHVKKSKILLGKAGVINVLAEDTPPPPPKSPDRDPFNISNDTYYTPKTEPTLRLKVGGNLIQHSTPVVELRAPFVPTHMGPMKLRAFHRPPLKKYSHGPMAQSIPHPVFPLLKTIAKKAKQREVERIASGGGDVFFMRNPEDLSGRDGDIVLAEFCEEHPPLINQVGMCSKIKNYYKRKAEKDSGPQDFVYGEVAFAHTSPFLGILHPGQCIQAIENNMYRAPIYPHKMAHNDFLVIRTRNNYWIRSVNSIYTVGQECPLYEVPGPNSKRANNFTRDFLQVFIYRLFWKSRDNPRRIRMDDIKQAFPAHSESSIRKRLKQCADFKRTGMDSNWWVIKPEFRLPSEEEIRAMVSPEQCCAYFSMIAAEQRLKDAGYGEKFLFAPQEDDDEEAQLKLDDEVKVAPWNTTRAYIQAMRGKCLLQLSGPADPTGCGEGFSYVRVPNKPTQTKEEQESQPKRSVTGTDADLRRLPLQRAKELLRQFKVPEEEIKKLSRWEVIDVVRTLSTEKAKAGEEGMDKFSRGNRFSIAEHQERYKEECQRIFDLQNRVLASSEVLSTDEAESSASEESDLEELGKNLENMLSNKKTSTQLSREREELERQELLRQLDEEHGGPSGSGGAKGAKGKDDPGQQMLATNNQGRILRITRTFRGNDGKEYTRVETVRRQPVIDAYIKIRTTKDEQFIKQFATLDEQQKEEMKREKRRIQEQLRRIKRNQERERLAQLAQNQKLQPGGMPTSLGDPKSSGGHSHKERDSGYKEVSPSRKKFKLKPDLKLKCGACGQVGHMRTNKACPLYSGMQSSLSQSNPSLADDFDEQSEKEMTMDDDDLVNVDGTKVTLSSKILKRHGGDDGKRRSGSSSGFTLKVPRDAMGKKKRRVGGDLHCDYLQRHNKTANRRRTDPVVVLSSILEIIHNELRSMPDVSPFLFPVSAKKVPDYYRVVTKPMDLQTMREYIRQRRYTSREMFLEDLKQIVDNSLIYNGPQSAYTLAAQRMFSSCFELLAEREDKLMRLEKAINPLLDDDDQVALSFIFDKLHSQIKQLPESWPFLKPVNKKQVKDYYTVIKRPMDLETIGKNIEAHRYHSRAEYLADIELIATNCEQYNGSDTRYTKFSKKILEYAQTQLIEFSEHCGQLENNIAKTQERARENAPEFDEAWGNDDYNFDRGSRASSPGDDYIDVEGHGGHASSSNSIHRSMGAEAGSSHTAPAVRKPAPPGPGEVKRGRGRPRKQRDPVEEDLQCSTDDEDDDEEEDFQEVSEDENNAASILDQGERINAPADAMDGMFDPKNIKTEIDLEAHQMADESMDVDPNYDPSDFLAMHKQRQSLGEPSSLQGAFTNFLSHEQDDNGPYNPAEASTSAASGADLGMDASMAMQMAPEMPVNTMNNGMGIDDDLDISESDEEDDGSRVRIKKEVFDDGDYALQHQQMGQAASQSQIYMVDSSNEPTTLDYQQPPQLDFQQVQEMEQLQHQVMPPMQSEQLQQQQTPQGDNDYAWTF

Target ORF information:

RefSeq Version	NM_057608.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057608.5

ORF Insert Sequence:

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA CTAAGGACGA CAAGGAACTA ATGCCTCCAC CAAGTGCACC AATGCGCTCC 
GGCTCTGGCG GCGGCATCGA GGAACCGGCC AAGTCAAATG ATGCATCTAG TCCCAGTGAC 
GATTCCAAAT CTACCGATTC AAAGGATGCG GATCGGAAGC TGGACACACC ACTGGCAGAC 
ATACTGCCGT CCAAGTACCA GAATGTGGAT GTGCGTGAGC TCTTTCCGGA CTTTCGTCCC 
CAAAAGGTGC TACGCTTCTC CCGTCTCTTC GGACCCGGTA AACCCACGAG TTTGCCCCAG 
ATCTGGCGAC ACGTGCGCAA ACGCCGCCGC AAGCGAAATC AATCCAGGGA TCAGAAGACG 
ACAAACACTG GTGGTTCGGA CTCTCCAAGC GATACTGAGG AGCCACGAAA GCGGGGCTTC 
AGTCTGCACT ATGCTGCAGA GCCAACGCCA GCGGAGTGCA TGTCCGACGA CGAGGACAAA 
TTGCTGGGCG ACTTCAACAG CGAGGATGTG CGTCCAGAAG GACCGGACAA CGGCGAGAAC 
AGCGATCAGA AGCCAAAGGT GGCTGACTGG CGGTTCGGGC CTGCACAGAT TTGGTATGAT 
ATGCTAGAGG TGCCCGACTC TGGAGAGGGG TTCAACTATG GCTTCAAGAC AAAGGCAGCA 
AGCACCTCGT CTCAGCCGCA ACTCAAAGAT GAACGCCGTG TAAAGAGTCC AGAAGATGAT 
GTCGAAGATC CAAGCATTGC GGATGATGCC TTTCTCATGG TCTCCCAGCT GCATTGGGAA 
GACGACGTGG TCTGGGACGG TAACGACATC AAGGCAAAGG TGCTCCAAAA GCTTAACTCA 
AAGACAAATG CAGCCGGATG GTTGCCCTCG AGCGGGTCGA GAACGGCTGG CGCCTTTAGC 
CAGCCGGGCA AACCGTCTAT GCCCGTGGGA AGTGGAAGCT CCAAGCAGGG TTCGGGAGCA 
TCAAGCAAAA AGGCCCAGCA AAATGCTCAA GCAAAGCCAG CGGAGGCACC TGATGACACC 
TGGTATAGCC TTTTTCCAGT TGAAAATGAG GAGCTAATAT ACTACAAGTG GGAGGACGAG 
GTAATCTGGG ATGCCCAGCA AGTGAGCAAG GTGCCCAAGC CGAAGGTACT CACTTTGGAT 
CCCAACGACG AAAACATCAT TTTGGGTATT CCTGATGACA TAGATCCCTC TAAAATCAAT 
AAGAGCACGG GTCCTCCACC TAAGATCAAG ATACCGCATC CTCACGTAAA AAAGTCTAAG 
ATCCTACTTG GCAAGGCGGG TGTGATCAAC GTGCTTGCGG AGGACACACC TCCGCCGCCA 
CCCAAAAGTC CTGACCGTGA CCCCTTTAAC ATATCCAATG ACACTTACTA CACACCGAAG 
ACAGAACCCA CCCTACGACT TAAGGTGGGC GGTAATCTTA TTCAACACTC GACTCCGGTG 
GTAGAGCTGC GAGCTCCGTT TGTGCCTACG CACATGGGCC CGATGAAGCT CCGCGCCTTC 
CATCGCCCCC CCCTAAAGAA GTACTCCCAC GGGCCAATGG CCCAGTCTAT CCCTCATCCT 
GTCTTCCCAC TGCTGAAGAC CATCGCAAAG AAGGCGAAGC AGCGCGAAGT GGAGCGCATT 
GCCTCCGGTG GCGGAGACGT CTTCTTTATG CGCAACCCAG AGGATTTAAG CGGAAGGGAC 
GGAGACATCG TACTAGCCGA GTTCTGCGAA GAGCATCCGC CTCTGATAAA CCAGGTGGGC 
ATGTGCTCCA AGATAAAGAA CTACTACAAG CGTAAGGCTG AGAAGGACAG TGGGCCGCAG 
GATTTTGTTT ATGGAGAAGT GGCCTTTGCG CACACAAGTC CCTTCCTGGG CATCCTGCAT 
CCTGGCCAGT GCATACAGGC GATTGAGAAC AACATGTACC GAGCGCCTAT TTATCCACAT 
AAGATGGCTC ACAACGATTT CCTCGTCATT CGCACGCGGA ACAACTACTG GATACGGTCG 
GTGAATTCAA TATACACCGT GGGTCAGGAG TGTCCGCTGT ACGAGGTACC CGGTCCGAAT 
TCCAAAAGGG CCAACAACTT CACCCGTGAC TTTCTGCAGG TGTTTATTTA CCGCCTGTTC 
TGGAAAAGTC GCGACAACCC GCGCCGCATT CGAATGGACG ATATAAAACA GGCTTTTCCC 
GCTCATTCCG AGAGCAGCAT CCGCAAGCGT TTAAAGCAGT GCGCTGACTT CAAGCGAACA 
GGCATGGACT CCAATTGGTG GGTTATAAAG CCAGAGTTTC GCCTTCCATC CGAGGAGGAG 
ATCCGAGCCA TGGTGTCACC TGAGCAGTGT TGCGCGTACT TCAGCATGAT AGCGGCGGAA 
CAACGCTTAA AGGATGCTGG GTATGGAGAA AAGTTTTTGT TCGCACCTCA GGAAGATGAC 
GACGAGGAGG CGCAGCTGAA GCTTGACGAC GAAGTAAAGG TGGCTCCTTG GAACACGACT 
CGCGCATATA TCCAAGCCAT GCGGGGAAAG TGTTTACTCC AGTTGAGTGG TCCAGCCGAT 
CCAACGGGAT GTGGAGAGGG ATTTTCATAT GTTCGAGTGC CAAACAAGCC CACGCAAACC 
AAGGAGGAGC AAGAGTCGCA GCCTAAACGT TCGGTCACAG GAACAGATGC AGATTTGCGT 
CGTCTGCCAC TCCAGCGTGC AAAAGAGCTG TTGCGGCAGT TCAAGGTGCC CGAGGAGGAG 
ATCAAAAAGC TTTCCCGCTG GGAGGTCATT GACGTGGTGC GCACCCTGTC CACAGAAAAG 
GCCAAGGCCG GTGAAGAGGG AATGGATAAG TTTTCTCGTG GCAACCGGTT CTCCATTGCA 
GAGCATCAGG AGCGTTATAA GGAAGAGTGC CAGCGCATAT TCGATCTGCA AAACAGAGTG 
CTGGCCAGCT CTGAGGTGCT GTCCACAGAT GAGGCAGAGT CCTCGGCCTC TGAGGAATCT 
GATCTCGAAG AACTTGGCAA GAATCTTGAG AACATGCTGT CAAACAAGAA AACCTCGACG 
CAATTGTCAA GGGAACGTGA AGAGCTGGAG CGTCAGGAGT TGCTTCGCCA GCTTGACGAA 
GAACACGGCG GACCAAGTGG TAGTGGAGGA GCCAAGGGAG CCAAAGGAAA GGATGATCCG 
GGACAGCAAA TGCTGGCAAC CAACAACCAG GGCAGGATCC TTCGCATTAC GCGTACCTTT 
AGAGGTAACG ATGGCAAGGA ATATACTCGC GTGGAGACTG TGCGGCGGCA ACCAGTTATC 
GACGCCTACA TCAAGATTCG CACCACTAAG GACGAGCAGT TCATCAAGCA GTTCGCAACG 
CTAGATGAGC AGCAGAAGGA GGAGATGAAG CGCGAAAAGA GACGCATTCA GGAGCAGCTA 
CGTCGCATCA AGCGCAACCA GGAGCGCGAA CGCCTGGCGC AGCTGGCCCA GAACCAGAAG 
CTTCAGCCAG GTGGCATGCC CACTTCCTTG GGTGATCCTA AGAGCTCGGG CGGTCATTCG 
CACAAGGAGC GGGATAGCGG CTACAAGGAG GTCAGCCCTT CGCGCAAGAA GTTCAAGCTT 
AAGCCAGACC TAAAGCTGAA GTGCGGCGCC TGTGGACAGG TTGGTCACAT GCGCACAAAC 
AAAGCCTGTC CCTTGTATTC TGGCATGCAA AGCAGTCTGT CCCAGTCGAA CCCATCTCTG 
GCTGACGATT TTGACGAACA GAGCGAAAAG GAGATGACAA TGGATGACGA TGATCTTGTG 
AATGTCGATG GCACCAAAGT AACGCTCAGC AGTAAGATTC TCAAGCGTCA TGGTGGTGAT 
GATGGCAAGC GTCGCAGCGG ATCTAGCTCT GGTTTCACCT TGAAGGTTCC CCGAGATGCG 
ATGGGCAAGA AGAAACGCAG AGTGGGTGGC GATCTTCATT GTGACTATCT GCAGCGACAC 
AATAAAACGG CCAATCGCAG GCGCACGGAC CCCGTTGTGG TACTGTCCTC TATCCTGGAG 
ATTATCCATA ATGAGCTGCG ATCTATGCCA GATGTATCGC CATTCCTGTT CCCGGTAAGC 
GCAAAAAAGG TTCCCGACTA CTACCGCGTG GTGACCAAGC CCATGGATCT GCAAACGATG 
AGGGAGTATA TTCGCCAAAG GCGCTACACG AGTCGCGAGA TGTTCCTCGA GGATCTCAAG 
CAGATTGTGG ACAACTCGCT GATCTACAAT GGACCGCAGA GTGCATACAC CTTGGCTGCC 
CAACGCATGT TCAGCAGTTG TTTTGAATTG CTCGCAGAGC GCGAAGACAA ACTGATGCGC 
CTCGAGAAGG CAATTAACCC GCTGCTGGAC GACGATGACC AAGTGGCACT CTCCTTTATC 
TTTGACAAGC TGCACTCGCA GATTAAGCAA TTACCAGAGA GCTGGCCTTT CCTTAAGCCT 
GTCAACAAGA AACAGGTTAA GGACTACTAC ACGGTTATCA AGCGACCCAT GGACCTCGAA 
ACTATCGGCA AAAACATTGA AGCTCATCGC TATCACAGTC GTGCCGAGTA TCTGGCTGAT 
ATCGAGTTGA TCGCCACCAA CTGTGAGCAG TACAACGGCA GTGACACCCG CTACACCAAG 
TTCTCAAAGA AGATACTTGA GTATGCCCAA ACCCAGTTAA TTGAGTTTTC GGAGCACTGC 
GGCCAGTTGG AAAATAACAT AGCTAAGACG CAGGAGCGTG CTAGGGAAAA TGCACCAGAG 
TTTGATGAAG CCTGGGGCAA TGATGATTAC AACTTTGACC GTGGCAGTAG GGCCAGTTCA 
CCCGGAGATG ACTACATCGA CGTCGAGGGT CATGGGGGGC ATGCCTCCTC ATCGAACTCT 
ATCCATCGCA GCATGGGCGC CGAGGCCGGT TCGTCACATA CGGCGCCGGC GGTGCGAAAA 
CCAGCTCCTC CTGGTCCTGG TGAGGTGAAG CGCGGAAGGG GTAGGCCCCG CAAGCAGCGC 
GACCCCGTGG AGGAGGATCT CCAATGCTCC ACAGATGACG AGGACGACGA CGAGGAGGAG 
GACTTCCAGG AGGTCTCCGA AGACGAGAAC AATGCGGCGA GCATTTTAGA TCAGGGCGAA 
CGTATCAATG CGCCTGCCGA TGCCATGGAT GGCATGTTTG ACCCCAAGAA CATCAAGACA 
GAGATTGACC TAGAGGCTCA CCAGATGGCA GATGAATCCA TGGATGTGGA CCCCAACTAC 
GATCCCTCAG ATTTCCTCGC CATGCACAAG CAGCGCCAGA GCCTCGGCGA GCCCAGCAGC 
TTGCAGGGTG CTTTCACCAA CTTCCTATCG CACGAGCAGG ATGATAATGG GCCTTACAAT 
CCCGCCGAAG CCAGCACAAG TGCCGCTTCC GGTGCAGACT TAGGAATGGA CGCTTCAATG 
GCCATGCAAA TGGCGCCGGA AATGCCTGTC AATACCATGA ACAACGGAAT GGGCATCGAT 
GATGATCTGG ATATTTCGGA GAGTGACGAG GAAGACGATG GTTCTCGAGT GCGTATCAAA 
AAGGAGGTCT TCGACGACGG GGATTACGCC TTGCAGCACC AGCAGATGGG ACAGGCAGCA 
TCGCAGTCGC AGATATACAT GGTGGATTCG TCCAACGAGC CCACGACTCT CGACTACCAG 
CAACCACCGC AACTGGACTT CCAACAAGTG CAGGAAATGG AGCAGTTGCA GCACCAAGTG 
ATGCCACCAA TGCAATCAGA GCAACTGCAG CAGCAACAGA CGCCGCAGGG AGACAATGAT 
TATGCCTGGA CTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Taf1 ( NM_001260023.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246952.1 Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant E, mRNA.

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CloneID	OFa07570
Clone ID Related Accession (Same CDS sequence)	NM_001260023.1
Accession Version	NM_001260023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TBP-associated factor 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA GAGAGGATAT TGCTCAAATT TTTAAAGGTC TATGCAATTC CTGTCAAAGT 
GACACGATTA CTTTTGCACC GCTCAGGTTG GGTTCAGACC TATTGCTATA CAGAGAGAAT 
AACTCACATT TTGTTGCAGC TAAGGACGAC AAGGAACTAA TGCCTCCACC AAGTGCACCA 
ATGCGCTCCG GCTCTGGCGG CGGCATCGAG GAACCGGCCA AGTCAAATGA TGCATCTAGT 
CCCAGTGACG ATTCCAAATC TACCGATTCA AAGGATGCGG ATCGGAAGCT GGACACACCA 
CTGGCAGACA TACTGCCGTC CAAGTACCAG AATGTGGATG TGCGTGAGCT CTTTCCGGAC 
TTTCGTCCCC AAAAGGTGCT ACGCTTCTCC CGTCTCTTCG GACCCGGTAA ACCCACGAGT 
TTGCCCCAGA TCTGGCGACA CGTGCGCAAA CGCCGCCGCA AGCGAAATCA ATCCAGGGAT 
CAGAAGACGA CAAACACTGG TGGTTCGGAC TCTCCAAGCG ATACTGAGGA GCCACGAAAG 
CGGGGCTTCA GTCTGCACTA TGCTGCAGAG CCAACGCCAG CGGAGTGCAT GTCCGACGAC 
GAGGACAAAT TGCTGGGCGA CTTCAACAGC GAGGATGTGC GTCCAGAAGG ACCGGACAAC 
GGCGAGAACA GCGATCAGAA GCCAAAGGTG GCTGACTGGC GGTTCGGGCC TGCACAGATT 
TGGTATGATA TGCTAGAGGT GCCCGACTCT GGAGAGGGGT TCAACTATGG CTTCAAGACA 
AAGGCAGCAA GCACCTCGTC TCAGCCGCAA CTCAAAGATG AACGCCGTGT AAAGAGTCCA 
GAAGATGATG TCGAAGATCC AAGCATTGCG GATGATGCCT TTCTCATGGT CTCCCAGCTG 
CATTGGGAAG ACGACGTGGT CTGGGACGGT AACGACATCA AGGCAAAGGT GCTCCAAAAG 
CTTAACTCAA AGACAAATGC AGCCGGATGG TTGCCCTCGA GCGGGTCGAG AACGGCTGGC 
GCCTTTAGCC AGCCGGGCAA ACCGTCTATG CCCGTGGGAA GTGGAAGCTC CAAGCAGGGT 
TCGGGAGCAT CAAGCAAAAA GGCCCAGCAA AATGCTCAAG CAAAGCCAGC GGAGGCACCT 
GATGACACCT GGTATAGCCT TTTTCCAGTT GAAAATGAGG AGCTAATATA CTACAAGTGG 
GAGGACGAGG TAATCTGGGA TGCCCAGCAA GTGAGCAAGG TGCCCAAGCC GAAGGTACTC 
ACTTTGGATC CCAACGACGA AAACATCATT TTGGGTATTC CTGATGACAT AGATCCCTCT 
AAAATCAATA AGAGCACGGG TCCTCCACCT AAGATCAAGA TACCGCATCC TCACGTAAAA 
AAGTCTAAGA TCCTACTTGG CAAGGCGGGT GTGATCAACG TGCTTGCGGA GGACACACCT 
CCGCCGCCAC CCAAAAGTCC TGACCGTGAC CCCTTTAACA TATCCAATGA CACTTACTAC 
ACACCGAAGA CAGAACCCAC CCTACGACTT AAGGTGGGCG GTAATCTTAT TCAACACTCG 
ACTCCGGTGG TAGAGCTGCG AGCTCCGTTT GTGCCTACGC ACATGGGCCC GATGAAGCTC 
CGCGCCTTCC ATCGCCCCCC CCTAAAGAAG TACTCCCACG GGCCAATGGC CCAGTCTATC 
CCTCATCCTG TCTTCCCACT GCTGAAGACC ATCGCAAAGA AGGCGAAGCA GCGCGAAGTG 
GAGCGCATTG CCTCCGGTGG CGGAGACGTC TTCTTTATGC GCAACCCAGA GGATTTAAGC 
GGAAGGGACG GAGACATCGT ACTAGCCGAG TTCTGCGAAG AGCATCCGCC TCTGATAAAC 
CAGGTGGGCA TGTGCTCCAA GATAAAGAAC TACTACAAGC GTAAGGCTGA GAAGGACAGT 
GGGCCGCAGG ATTTTGTTTA TGGAGAAGTG GCCTTTGCGC ACACAAGTCC CTTCCTGGGC 
ATCCTGCATC CTGGCCAGTG CATACAGGCG ATTGAGAACA ACATGTACCG AGCGCCTATT 
TATCCACATA AGATGGCTCA CAACGATTTC CTCGTCATTC GCACGCGGAA CAACTACTGG 
ATACGGTCGG TGAATTCAAT ATACACCGTG GGTCAGGAGT GTCCGCTGTA CGAGGTACCC 
GGTCCGAATT CCAAAAGGGC CAACAACTTC ACCCGTGACT TTCTGCAGGT GTTTATTTAC 
CGCCTGTTCT GGAAAAGTCG CGACAACCCG CGCCGCATTC GAATGGACGA TATAAAACAG 
GCTTTTCCCG CTCATTCCGA GAGCAGCATC CGCAAGCGTT TAAAGCAGTG CGCTGACTTC 
AAGCGAACAG GCATGGACTC CAATTGGTGG GTTATAAAGC CAGAGTTTCG CCTTCCATCC 
GAGGAGGAGA TCCGAGCCAT GGTGTCACCT GAGCAGTGTT GCGCGTACTT CAGCATGATA 
GCGGCGGAAC AACGCTTAAA GGATGCTGGG TATGGAGAAA AGTTTTTGTT CGCACCTCAG 
GAAGATGACG ACGAGGAGGC GCAGCTGAAG CTTGACGACG AAGTAAAGGT GGCTCCTTGG 
AACACGACTC GCGCATATAT CCAAGCCATG CGGGGAAAGT GTTTACTCCA GTTGAGTGGT 
CCAGCCGATC CAACGGGATG TGGAGAGGGA TTTTCATATG TTCGAGTGCC AAACAAGCCC 
ACGCAAACCA AGGAGGAGCA AGAGTCGCAG CCTAAACGTT CGGTCACAGG AACAGATGCA 
GATTTGCGTC GTCTGCCACT CCAGCGTGCA AAAGAGCTGT TGCGGCAGTT CAAGGTGCCC 
GAGGAGGAGA TCAAAAAGCT TTCCCGCTGG GAGGTCATTG ACGTGGTGCG CACCCTGTCC 
ACAGAAAAGG CCAAGGCCGG TGAAGAGGGA ATGGATAAGT TTTCTCGTGG CAACCGGTTC 
TCCATTGCAG AGCATCAGGA GCGTTATAAG GAAGAGTGCC AGCGCATATT CGATCTGCAA 
AACAGAGTGC TGGCCAGCTC TGAGGTGCTG TCCACAGATG AGGCAGAGTC CTCGGCCTCT 
GAGGAATCTG ATCTCGAAGA ACTTGGCAAG AATCTTGAGA ACATGCTGTC AAACAAGAAA 
ACCTCGACGC AATTGTCAAG GGAACGTGAA GAGCTGGAGC GTCAGGAGTT GCTTCGCCAG 
CTTGACGAAG AACACGGCGG ACCAAGTGGT AGTGGAGGAG CCAAGGGAGC CAAAGGAAAG 
GATGATCCGG GACAGCAAAT GCTGGCAACC AACAACCAGG GCAGGATCCT TCGCATTACG 
CGTACCTTTA GAGGTAACGA TGGCAAGGAA TATACTCGCG TGGAGACTGT GCGGCGGCAA 
CCAGTTATCG ACGCCTACAT CAAGATTCGC ACCACTAAGG ACGAGCAGTT CATCAAGCAG 
TTCGCAACGC TAGATGAGCA GCAGAAGGAG GAGATGAAGC GCGAAAAGAG ACGCATTCAG 
GAGCAGCTAC GTCGCATCAA GCGCAACCAG GAGCGCGAAC GCCTGGCGCA GCTGGCCCAG 
AACCAGAAGC TTCAGCCAGG TGGCATGCCC ACTTCCTTGG GTGATCCTAA GAGCTCGGGC 
GGTCATTCGC ACAAGGAGCG GGATAGCGGC TACAAGGAGG TCAGCCCTTC GCGCAAGAAG 
TTCAAGCTTA AGCCAGACCT AAAGCTGAAG TGCGGCGCCT GTGGACAGGT TGGTCACATG 
CGCACAAACA AAGCCTGTCC CTTGTATTCT GGCATGCAAA GCAGTCTGTC CCAGTCGAAC 
CCATCTCTGG CTGACGATTT TGACGAACAG AGCGAAAAGG AGATGACAAT GGATGACGAT 
GATCTTGTGA ATGTCGATGG CACCAAAGTA ACGCTCAGCA GTAAGATTCT CAAGCGTCAT 
GGTGGTGATG ATGGCAAGCG TCGCAGCGGA TCTAGCTCTG GTTTCACCTT GAAGGTTCCC 
CGAGATGCGA TGGGCAAGAA GAAACGCAGA GTGGGTGGCG ATCTTCATTG TGACTATCTG 
CAGCGACACA ATAAAACGGC CAATCGCAGG CGCACGGACC CCGTTGTGGT ACTGTCCTCT 
ATCCTGGAGA TTATCCATAA TGAGCTGCGA TCTATGCCAG ATGTATCGCC ATTCCTGTTC 
CCGGTAAGCG CAAAAAAGGT TCCCGACTAC TACCGCGTGG TGACCAAGCC CATGGATCTG 
CAAACGATGA GGGAGTATAT TCGCCAAAGG CGCTACACGA GTCGCGAGAT GTTCCTCGAG 
GATCTCAAGC AGATTGTGGA CAACTCGCTG ATCTACAATG GACCGCAGAG TGCATACACC 
TTGGCTGCCC AACGCATGTT CAGCAGTTGT TTTGAATTGC TCGCAGAGCG CGAAGACAAA 
CTGATGCGCC TCGAGAAGGC AATTAACCCG CTGCTGGACG ACGATGACCA AGTGGCACTC 
TCCTTTATCT TTGACAAGCT GCACTCGCAG ATTAAGCAAT TACCAGAGAG CTGGCCTTTC 
CTTAAGCCTG TCAACAAGAA ACAGGTTAAG GACTACTACA CGGTTATCAA GCGACCCATG 
GACCTCGAAA CTATCGGCAA AAACATTGAA GCTCATCGCT ATCACAGTCG TGCCGAGTAT 
CTGGCTGATA TCGAGTTGAT CGCCACCAAC TGTGAGCAGT ACAACGGCAG TGACACCCGC 
TACACCAAGT TCTCAAAGAA GATACTTGAG TATGCCCAAA CCCAGTTAAT TGAGTTTTCG 
GAGCACTGCG GCCAGTTGGA AAATAACATA GCTAAGACGC AGGAGCGTGC TAGGGAAAAT 
GCACCAGAGT TTGATGAAGC CTGGGGCAAT GATGATTACA ACTTTGACCG TGGCAGTAGG 
GCCAGTTCAC CCGGAGATGA CTACATCGAC GTCGAGGGTC ATGGGGGGCA TGCCTCCTCA 
TCGAACTCTA TCCATCGCAG CATGGGCGCC GAGGCCGGTT CGTCACATAC GGCGCCGGCG 
GTGCGAAAAC CAGCTCCTCC TGGTCCTGGT GAGGTGAAGC GCGGAAGGGG TAGGCCCCGC 
AAGCAGCGCG ACCCCGTGGA GGAGGTCAAA TCCCAGAATC CGGTTAAGCG TGGTCGGGGG 
CGTCCGAGGA AGGACAGCCT TGCCTCAAAC ATGAGTCACA CGCAAGCTTA CTTCCTGGAT 
GAAGATCTCC AATGCTCCAC AGATGACGAG GACGACGACG AGGAGGAGGA CTTCCAGGAG 
GTCTCCGAAG ACGAGAACAA TGCGGCGAGC ATTTTAGATC AGGGCGAACG TATCAATGCG 
CCTGCCGATG CCATGGATGG CATGTTTGAC CCCAAGAACA TCAAGACAGA GATTGACCTA 
GAGGCTCACC AGATGGCAGA GGAGCCGATC GGCGAGGATG ACAGCCAGCA GGTGGCCGAA 
GCAATGGTGC AGTTGAGTGG CGTGGGCGGC TACTATGCTC AACAGCAGCA AGATGAATCC 
ATGGATGTGG ACCCCAACTA CGATCCCTCA GATTTCCTCG CCATGCACAA GCAGCGCCAG 
AGCCTCGGCG AGCCCAGCAG CTTGCAGGGT GCTTTCACCA ACTTCCTATC GCACGAGCAG 
GATGATAATG GGCCTTACAA TCCCGCCGAA GCCAGCACAA GTGCCGCTTC CGGTGCAGAC 
TTAGGAATGG ACGCTTCAAT GGCCATGCAA ATGGCGCCGG AAATGCCTGT CAATACCATG 
AACAACGGAA TGGGCATCGA TGATGATCTG GATATTTCGG AGAGTGACGA GGAAGACGAT 
GGTTCTCGAG TGCGTATCAA AAAGGAGGTC TTCGACGACG GGGATTACGC CTTGCAGCAC 
CAGCAGATGG GACAGGCAGC ATCGCAGTCG CAGATATACA TGGTGGATTC GTCCAACGAG 
CCCACGACTC TCGACTACCA GCAACCACCG CAACTGGACT TCCAACAAGT GCAGGAAATG 
GAGCAGTTGC AGCACCAAGT GATGCCACCA ATGCAATCAG AGCAACTGCA GCAGCAACAG 
ACGCCGCAGG GAGACAATGA TTATGCCTGG ACTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246952.1
CDS	102..6620
Translation	MEMESDNSDDEGSIGNGLDLTGILFGNIDSEGRLLQDDDGEGRGGTGFDAELRENIGSLSKLGLDSMLLEVIDLKEAEPPSDDEEEEDARPSAVSASEGMSAFDALKAGVKREDGAVKAQDDAIDYSDITELSEDCPRTPPEETSTYDDLEDAIPASKVEAKLREDIAQIFKGLCNSCQSDTITFAPLRLGSDLLLYRENNSHFVAAKDDKELMPPPSAPMRSGSGGGIEEPAKSNDASSPSDDSKSTDSKDADRKLDTPLADILPSKYQNVDVRELFPDFRPQKVLRFSRLFGPGKPTSLPQIWRHVRKRRRKRNQSRDQKTTNTGGSDSPSDTEEPRKRGFSLHYAAEPTPAECMSDDEDKLLGDFNSEDVRPEGPDNGENSDQKPKVADWRFGPAQIWYDMLEVPDSGEGFNYGFKTKAASTSSQPQLKDERRVKSPEDDVEDPSIADDAFLMVSQLHWEDDVVWDGNDIKAKVLQKLNSKTNAAGWLPSSGSRTAGAFSQPGKPSMPVGSGSSKQGSGASSKKAQQNAQAKPAEAPDDTWYSLFPVENEELIYYKWEDEVIWDAQQVSKVPKPKVLTLDPNDENIILGIPDDIDPSKINKSTGPPPKIKIPHPHVKKSKILLGKAGVINVLAEDTPPPPPKSPDRDPFNISNDTYYTPKTEPTLRLKVGGNLIQHSTPVVELRAPFVPTHMGPMKLRAFHRPPLKKYSHGPMAQSIPHPVFPLLKTIAKKAKQREVERIASGGGDVFFMRNPEDLSGRDGDIVLAEFCEEHPPLINQVGMCSKIKNYYKRKAEKDSGPQDFVYGEVAFAHTSPFLGILHPGQCIQAIENNMYRAPIYPHKMAHNDFLVIRTRNNYWIRSVNSIYTVGQECPLYEVPGPNSKRANNFTRDFLQVFIYRLFWKSRDNPRRIRMDDIKQAFPAHSESSIRKRLKQCADFKRTGMDSNWWVIKPEFRLPSEEEIRAMVSPEQCCAYFSMIAAEQRLKDAGYGEKFLFAPQEDDDEEAQLKLDDEVKVAPWNTTRAYIQAMRGKCLLQLSGPADPTGCGEGFSYVRVPNKPTQTKEEQESQPKRSVTGTDADLRRLPLQRAKELLRQFKVPEEEIKKLSRWEVIDVVRTLSTEKAKAGEEGMDKFSRGNRFSIAEHQERYKEECQRIFDLQNRVLASSEVLSTDEAESSASEESDLEELGKNLENMLSNKKTSTQLSREREELERQELLRQLDEEHGGPSGSGGAKGAKGKDDPGQQMLATNNQGRILRITRTFRGNDGKEYTRVETVRRQPVIDAYIKIRTTKDEQFIKQFATLDEQQKEEMKREKRRIQEQLRRIKRNQERERLAQLAQNQKLQPGGMPTSLGDPKSSGGHSHKERDSGYKEVSPSRKKFKLKPDLKLKCGACGQVGHMRTNKACPLYSGMQSSLSQSNPSLADDFDEQSEKEMTMDDDDLVNVDGTKVTLSSKILKRHGGDDGKRRSGSSSGFTLKVPRDAMGKKKRRVGGDLHCDYLQRHNKTANRRRTDPVVVLSSILEIIHNELRSMPDVSPFLFPVSAKKVPDYYRVVTKPMDLQTMREYIRQRRYTSREMFLEDLKQIVDNSLIYNGPQSAYTLAAQRMFSSCFELLAEREDKLMRLEKAINPLLDDDDQVALSFIFDKLHSQIKQLPESWPFLKPVNKKQVKDYYTVIKRPMDLETIGKNIEAHRYHSRAEYLADIELIATNCEQYNGSDTRYTKFSKKILEYAQTQLIEFSEHCGQLENNIAKTQERARENAPEFDEAWGNDDYNFDRGSRASSPGDDYIDVEGHGGHASSSNSIHRSMGAEAGSSHTAPAVRKPAPPGPGEVKRGRGRPRKQRDPVEEVKSQNPVKRGRGRPRKDSLASNMSHTQAYFLDEDLQCSTDDEDDDEEEDFQEVSEDENNAASILDQGERINAPADAMDGMFDPKNIKTEIDLEAHQMAEEPIGEDDSQQVAEAMVQLSGVGGYYAQQQQDESMDVDPNYDPSDFLAMHKQRQSLGEPSSLQGAFTNFLSHEQDDNGPYNPAEASTSAASGADLGMDASMAMQMAPEMPVNTMNNGMGIDDDLDISESDEEDDGSRVRIKKEVFDDGDYALQHQQMGQAASQSQIYMVDSSNEPTTLDYQQPPQLDFQQVQEMEQLQHQVMPPMQSEQLQQQQTPQGDNDYAWTF

Target ORF information:

RefSeq Version	NM_001260023.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260023.1

ORF Insert Sequence:

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA GAGAGGATAT TGCTCAAATT TTTAAAGGTC TATGCAATTC CTGTCAAAGT 
GACACGATTA CTTTTGCACC GCTCAGGTTG GGTTCAGACC TATTGCTATA CAGAGAGAAT 
AACTCACATT TTGTTGCAGC TAAGGACGAC AAGGAACTAA TGCCTCCACC AAGTGCACCA 
ATGCGCTCCG GCTCTGGCGG CGGCATCGAG GAACCGGCCA AGTCAAATGA TGCATCTAGT 
CCCAGTGACG ATTCCAAATC TACCGATTCA AAGGATGCGG ATCGGAAGCT GGACACACCA 
CTGGCAGACA TACTGCCGTC CAAGTACCAG AATGTGGATG TGCGTGAGCT CTTTCCGGAC 
TTTCGTCCCC AAAAGGTGCT ACGCTTCTCC CGTCTCTTCG GACCCGGTAA ACCCACGAGT 
TTGCCCCAGA TCTGGCGACA CGTGCGCAAA CGCCGCCGCA AGCGAAATCA ATCCAGGGAT 
CAGAAGACGA CAAACACTGG TGGTTCGGAC TCTCCAAGCG ATACTGAGGA GCCACGAAAG 
CGGGGCTTCA GTCTGCACTA TGCTGCAGAG CCAACGCCAG CGGAGTGCAT GTCCGACGAC 
GAGGACAAAT TGCTGGGCGA CTTCAACAGC GAGGATGTGC GTCCAGAAGG ACCGGACAAC 
GGCGAGAACA GCGATCAGAA GCCAAAGGTG GCTGACTGGC GGTTCGGGCC TGCACAGATT 
TGGTATGATA TGCTAGAGGT GCCCGACTCT GGAGAGGGGT TCAACTATGG CTTCAAGACA 
AAGGCAGCAA GCACCTCGTC TCAGCCGCAA CTCAAAGATG AACGCCGTGT AAAGAGTCCA 
GAAGATGATG TCGAAGATCC AAGCATTGCG GATGATGCCT TTCTCATGGT CTCCCAGCTG 
CATTGGGAAG ACGACGTGGT CTGGGACGGT AACGACATCA AGGCAAAGGT GCTCCAAAAG 
CTTAACTCAA AGACAAATGC AGCCGGATGG TTGCCCTCGA GCGGGTCGAG AACGGCTGGC 
GCCTTTAGCC AGCCGGGCAA ACCGTCTATG CCCGTGGGAA GTGGAAGCTC CAAGCAGGGT 
TCGGGAGCAT CAAGCAAAAA GGCCCAGCAA AATGCTCAAG CAAAGCCAGC GGAGGCACCT 
GATGACACCT GGTATAGCCT TTTTCCAGTT GAAAATGAGG AGCTAATATA CTACAAGTGG 
GAGGACGAGG TAATCTGGGA TGCCCAGCAA GTGAGCAAGG TGCCCAAGCC GAAGGTACTC 
ACTTTGGATC CCAACGACGA AAACATCATT TTGGGTATTC CTGATGACAT AGATCCCTCT 
AAAATCAATA AGAGCACGGG TCCTCCACCT AAGATCAAGA TACCGCATCC TCACGTAAAA 
AAGTCTAAGA TCCTACTTGG CAAGGCGGGT GTGATCAACG TGCTTGCGGA GGACACACCT 
CCGCCGCCAC CCAAAAGTCC TGACCGTGAC CCCTTTAACA TATCCAATGA CACTTACTAC 
ACACCGAAGA CAGAACCCAC CCTACGACTT AAGGTGGGCG GTAATCTTAT TCAACACTCG 
ACTCCGGTGG TAGAGCTGCG AGCTCCGTTT GTGCCTACGC ACATGGGCCC GATGAAGCTC 
CGCGCCTTCC ATCGCCCCCC CCTAAAGAAG TACTCCCACG GGCCAATGGC CCAGTCTATC 
CCTCATCCTG TCTTCCCACT GCTGAAGACC ATCGCAAAGA AGGCGAAGCA GCGCGAAGTG 
GAGCGCATTG CCTCCGGTGG CGGAGACGTC TTCTTTATGC GCAACCCAGA GGATTTAAGC 
GGAAGGGACG GAGACATCGT ACTAGCCGAG TTCTGCGAAG AGCATCCGCC TCTGATAAAC 
CAGGTGGGCA TGTGCTCCAA GATAAAGAAC TACTACAAGC GTAAGGCTGA GAAGGACAGT 
GGGCCGCAGG ATTTTGTTTA TGGAGAAGTG GCCTTTGCGC ACACAAGTCC CTTCCTGGGC 
ATCCTGCATC CTGGCCAGTG CATACAGGCG ATTGAGAACA ACATGTACCG AGCGCCTATT 
TATCCACATA AGATGGCTCA CAACGATTTC CTCGTCATTC GCACGCGGAA CAACTACTGG 
ATACGGTCGG TGAATTCAAT ATACACCGTG GGTCAGGAGT GTCCGCTGTA CGAGGTACCC 
GGTCCGAATT CCAAAAGGGC CAACAACTTC ACCCGTGACT TTCTGCAGGT GTTTATTTAC 
CGCCTGTTCT GGAAAAGTCG CGACAACCCG CGCCGCATTC GAATGGACGA TATAAAACAG 
GCTTTTCCCG CTCATTCCGA GAGCAGCATC CGCAAGCGTT TAAAGCAGTG CGCTGACTTC 
AAGCGAACAG GCATGGACTC CAATTGGTGG GTTATAAAGC CAGAGTTTCG CCTTCCATCC 
GAGGAGGAGA TCCGAGCCAT GGTGTCACCT GAGCAGTGTT GCGCGTACTT CAGCATGATA 
GCGGCGGAAC AACGCTTAAA GGATGCTGGG TATGGAGAAA AGTTTTTGTT CGCACCTCAG 
GAAGATGACG ACGAGGAGGC GCAGCTGAAG CTTGACGACG AAGTAAAGGT GGCTCCTTGG 
AACACGACTC GCGCATATAT CCAAGCCATG CGGGGAAAGT GTTTACTCCA GTTGAGTGGT 
CCAGCCGATC CAACGGGATG TGGAGAGGGA TTTTCATATG TTCGAGTGCC AAACAAGCCC 
ACGCAAACCA AGGAGGAGCA AGAGTCGCAG CCTAAACGTT CGGTCACAGG AACAGATGCA 
GATTTGCGTC GTCTGCCACT CCAGCGTGCA AAAGAGCTGT TGCGGCAGTT CAAGGTGCCC 
GAGGAGGAGA TCAAAAAGCT TTCCCGCTGG GAGGTCATTG ACGTGGTGCG CACCCTGTCC 
ACAGAAAAGG CCAAGGCCGG TGAAGAGGGA ATGGATAAGT TTTCTCGTGG CAACCGGTTC 
TCCATTGCAG AGCATCAGGA GCGTTATAAG GAAGAGTGCC AGCGCATATT CGATCTGCAA 
AACAGAGTGC TGGCCAGCTC TGAGGTGCTG TCCACAGATG AGGCAGAGTC CTCGGCCTCT 
GAGGAATCTG ATCTCGAAGA ACTTGGCAAG AATCTTGAGA ACATGCTGTC AAACAAGAAA 
ACCTCGACGC AATTGTCAAG GGAACGTGAA GAGCTGGAGC GTCAGGAGTT GCTTCGCCAG 
CTTGACGAAG AACACGGCGG ACCAAGTGGT AGTGGAGGAG CCAAGGGAGC CAAAGGAAAG 
GATGATCCGG GACAGCAAAT GCTGGCAACC AACAACCAGG GCAGGATCCT TCGCATTACG 
CGTACCTTTA GAGGTAACGA TGGCAAGGAA TATACTCGCG TGGAGACTGT GCGGCGGCAA 
CCAGTTATCG ACGCCTACAT CAAGATTCGC ACCACTAAGG ACGAGCAGTT CATCAAGCAG 
TTCGCAACGC TAGATGAGCA GCAGAAGGAG GAGATGAAGC GCGAAAAGAG ACGCATTCAG 
GAGCAGCTAC GTCGCATCAA GCGCAACCAG GAGCGCGAAC GCCTGGCGCA GCTGGCCCAG 
AACCAGAAGC TTCAGCCAGG TGGCATGCCC ACTTCCTTGG GTGATCCTAA GAGCTCGGGC 
GGTCATTCGC ACAAGGAGCG GGATAGCGGC TACAAGGAGG TCAGCCCTTC GCGCAAGAAG 
TTCAAGCTTA AGCCAGACCT AAAGCTGAAG TGCGGCGCCT GTGGACAGGT TGGTCACATG 
CGCACAAACA AAGCCTGTCC CTTGTATTCT GGCATGCAAA GCAGTCTGTC CCAGTCGAAC 
CCATCTCTGG CTGACGATTT TGACGAACAG AGCGAAAAGG AGATGACAAT GGATGACGAT 
GATCTTGTGA ATGTCGATGG CACCAAAGTA ACGCTCAGCA GTAAGATTCT CAAGCGTCAT 
GGTGGTGATG ATGGCAAGCG TCGCAGCGGA TCTAGCTCTG GTTTCACCTT GAAGGTTCCC 
CGAGATGCGA TGGGCAAGAA GAAACGCAGA GTGGGTGGCG ATCTTCATTG TGACTATCTG 
CAGCGACACA ATAAAACGGC CAATCGCAGG CGCACGGACC CCGTTGTGGT ACTGTCCTCT 
ATCCTGGAGA TTATCCATAA TGAGCTGCGA TCTATGCCAG ATGTATCGCC ATTCCTGTTC 
CCGGTAAGCG CAAAAAAGGT TCCCGACTAC TACCGCGTGG TGACCAAGCC CATGGATCTG 
CAAACGATGA GGGAGTATAT TCGCCAAAGG CGCTACACGA GTCGCGAGAT GTTCCTCGAG 
GATCTCAAGC AGATTGTGGA CAACTCGCTG ATCTACAATG GACCGCAGAG TGCATACACC 
TTGGCTGCCC AACGCATGTT CAGCAGTTGT TTTGAATTGC TCGCAGAGCG CGAAGACAAA 
CTGATGCGCC TCGAGAAGGC AATTAACCCG CTGCTGGACG ACGATGACCA AGTGGCACTC 
TCCTTTATCT TTGACAAGCT GCACTCGCAG ATTAAGCAAT TACCAGAGAG CTGGCCTTTC 
CTTAAGCCTG TCAACAAGAA ACAGGTTAAG GACTACTACA CGGTTATCAA GCGACCCATG 
GACCTCGAAA CTATCGGCAA AAACATTGAA GCTCATCGCT ATCACAGTCG TGCCGAGTAT 
CTGGCTGATA TCGAGTTGAT CGCCACCAAC TGTGAGCAGT ACAACGGCAG TGACACCCGC 
TACACCAAGT TCTCAAAGAA GATACTTGAG TATGCCCAAA CCCAGTTAAT TGAGTTTTCG 
GAGCACTGCG GCCAGTTGGA AAATAACATA GCTAAGACGC AGGAGCGTGC TAGGGAAAAT 
GCACCAGAGT TTGATGAAGC CTGGGGCAAT GATGATTACA ACTTTGACCG TGGCAGTAGG 
GCCAGTTCAC CCGGAGATGA CTACATCGAC GTCGAGGGTC ATGGGGGGCA TGCCTCCTCA 
TCGAACTCTA TCCATCGCAG CATGGGCGCC GAGGCCGGTT CGTCACATAC GGCGCCGGCG 
GTGCGAAAAC CAGCTCCTCC TGGTCCTGGT GAGGTGAAGC GCGGAAGGGG TAGGCCCCGC 
AAGCAGCGCG ACCCCGTGGA GGAGGTCAAA TCCCAGAATC CGGTTAAGCG TGGTCGGGGG 
CGTCCGAGGA AGGACAGCCT TGCCTCAAAC ATGAGTCACA CGCAAGCTTA CTTCCTGGAT 
GAAGATCTCC AATGCTCCAC AGATGACGAG GACGACGACG AGGAGGAGGA CTTCCAGGAG 
GTCTCCGAAG ACGAGAACAA TGCGGCGAGC ATTTTAGATC AGGGCGAACG TATCAATGCG 
CCTGCCGATG CCATGGATGG CATGTTTGAC CCCAAGAACA TCAAGACAGA GATTGACCTA 
GAGGCTCACC AGATGGCAGA GGAGCCGATC GGCGAGGATG ACAGCCAGCA GGTGGCCGAA 
GCAATGGTGC AGTTGAGTGG CGTGGGCGGC TACTATGCTC AACAGCAGCA AGATGAATCC 
ATGGATGTGG ACCCCAACTA CGATCCCTCA GATTTCCTCG CCATGCACAA GCAGCGCCAG 
AGCCTCGGCG AGCCCAGCAG CTTGCAGGGT GCTTTCACCA ACTTCCTATC GCACGAGCAG 
GATGATAATG GGCCTTACAA TCCCGCCGAA GCCAGCACAA GTGCCGCTTC CGGTGCAGAC 
TTAGGAATGG ACGCTTCAAT GGCCATGCAA ATGGCGCCGG AAATGCCTGT CAATACCATG 
AACAACGGAA TGGGCATCGA TGATGATCTG GATATTTCGG AGAGTGACGA GGAAGACGAT 
GGTTCTCGAG TGCGTATCAA AAAGGAGGTC TTCGACGACG GGGATTACGC CTTGCAGCAC 
CAGCAGATGG GACAGGCAGC ATCGCAGTCG CAGATATACA TGGTGGATTC GTCCAACGAG 
CCCACGACTC TCGACTACCA GCAACCACCG CAACTGGACT TCCAACAAGT GCAGGAAATG 
GAGCAGTTGC AGCACCAAGT GATGCCACCA ATGCAATCAG AGCAACTGCA GCAGCAACAG 
ACGCCGCAGG GAGACAATGA TTATGCCTGG ACTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Taf1 ( NM_001170061.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163532.1 Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant D, mRNA.

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CloneID	OFa07571
Clone ID Related Accession (Same CDS sequence)	NM_001170061.1
Accession Version	NM_001170061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TBP-associated factor 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA CTAAGGACGA CAAGGAACTA ATGCCTCCAC CAAGTGCACC AATGCGCTCC 
GGCTCTGGCG GCGGCATCGA GGAACCGGCC AAGTCAAATG ATGCATCTAG TCCCAGTGAC 
GATTCCAAAT CTACCGATTC AAAGGATGCG GATCGGAAGC TGGACACACC ACTGGCAGAC 
ATACTGCCGT CCAAGTACCA GAATGTGGAT GTGCGTGAGC TCTTTCCGGA CTTTCGTCCC 
CAAAAGGTGC TACGCTTCTC CCGTCTCTTC GGACCCGGTA AACCCACGAG TTTGCCCCAG 
ATCTGGCGAC ACGTGCGCAA ACGCCGCCGC AAGCGAAATC AATCCAGGGA TCAGAAGACG 
ACAAACACTG GTGGTTCGGA CTCTCCAAGC GATACTGAGG AGCCACGAAA GCGGGGCTTC 
AGTCTGCACT ATGCTGCAGA GCCAACGCCA GCGGAGTGCA TGTCCGACGA CGAGGACAAA 
TTGCTGGGCG ACTTCAACAG CGAGGATGTG CGTCCAGAAG GACCGGACAA CGGCGAGAAC 
AGCGATCAGA AGCCAAAGGT GGCTGACTGG CGGTTCGGGC CTGCACAGAT TTGGTATGAT 
ATGCTAGAGG TGCCCGACTC TGGAGAGGGG TTCAACTATG GCTTCAAGAC AAAGGCAGCA 
AGCACCTCGT CTCAGCCGCA ACTCAAAGAT GAACGCCGTG TAAAGAGTCC AGAAGATGAT 
GTCGAAGATC CAAGCATTGC GGATGATGCC TTTCTCATGG TCTCCCAGCT GCATTGGGAA 
GACGACGTGG TCTGGGACGG TAACGACATC AAGGCAAAGG TGCTCCAAAA GCTTAACTCA 
AAGACAAATG CAGCCGGATG GTTGCCCTCG AGCGGGTCGA GAACGGCTGG CGCCTTTAGC 
CAGCCGGGCA AACCGTCTAT GCCCGTGGGA AGTGGAAGCT CCAAGCAGGG TTCGGGAGCA 
TCAAGCAAAA AGGCCCAGCA AAATGCTCAA GCAAAGCCAG CGGAGGCACC TGATGACACC 
TGGTATAGCC TTTTTCCAGT TGAAAATGAG GAGCTAATAT ACTACAAGTG GGAGGACGAG 
GTAATCTGGG ATGCCCAGCA AGTGAGCAAG GTGCCCAAGC CGAAGGTACT CACTTTGGAT 
CCCAACGACG AAAACATCAT TTTGGGTATT CCTGATGACA TAGATCCCTC TAAAATCAAT 
AAGAGCACGG GTCCTCCACC TAAGATCAAG ATACCGCATC CTCACGTAAA AAAGTCTAAG 
ATCCTACTTG GCAAGGCGGG TGTGATCAAC GTGCTTGCGG AGGACACACC TCCGCCGCCA 
CCCAAAAGTC CTGACCGTGA CCCCTTTAAC ATATCCAATG ACACTTACTA CACACCGAAG 
ACAGAACCCA CCCTACGACT TAAGGTGGGC GGTAATCTTA TTCAACACTC GACTCCGGTG 
GTAGAGCTGC GAGCTCCGTT TGTGCCTACG CACATGGGCC CGATGAAGCT CCGCGCCTTC 
CATCGCCCCC CCCTAAAGAA GTACTCCCAC GGGCCAATGG CCCAGTCTAT CCCTCATCCT 
GTCTTCCCAC TGCTGAAGAC CATCGCAAAG AAGGCGAAGC AGCGCGAAGT GGAGCGCATT 
GCCTCCGGTG GCGGAGACGT CTTCTTTATG CGCAACCCAG AGGATTTAAG CGGAAGGGAC 
GGAGACATCG TACTAGCCGA GTTCTGCGAA GAGCATCCGC CTCTGATAAA CCAGGTGGGC 
ATGTGCTCCA AGATAAAGAA CTACTACAAG CGTAAGGCTG AGAAGGACAG TGGGCCGCAG 
GATTTTGTTT ATGGAGAAGT GGCCTTTGCG CACACAAGTC CCTTCCTGGG CATCCTGCAT 
CCTGGCCAGT GCATACAGGC GATTGAGAAC AACATGTACC GAGCGCCTAT TTATCCACAT 
AAGATGGCTC ACAACGATTT CCTCGTCATT CGCACGCGGA ACAACTACTG GATACGGTCG 
GTGAATTCAA TATACACCGT GGGTCAGGAG TGTCCGCTGT ACGAGGTACC CGGTCCGAAT 
TCCAAAAGGG CCAACAACTT CACCCGTGAC TTTCTGCAGG TGTTTATTTA CCGCCTGTTC 
TGGAAAAGTC GCGACAACCC GCGCCGCATT CGAATGGACG ATATAAAACA GGCTTTTCCC 
GCTCATTCCG AGAGCAGCAT CCGCAAGCGT TTAAAGCAGT GCGCTGACTT CAAGCGAACA 
GGCATGGACT CCAATTGGTG GGTTATAAAG CCAGAGTTTC GCCTTCCATC CGAGGAGGAG 
ATCCGAGCCA TGGTGTCACC TGAGCAGTGT TGCGCGTACT TCAGCATGAT AGCGGCGGAA 
CAACGCTTAA AGGATGCTGG GTATGGAGAA AAGTTTTTGT TCGCACCTCA GGAAGATGAC 
GACGAGGAGG CGCAGCTGAA GCTTGACGAC GAAGTAAAGG TGGCTCCTTG GAACACGACT 
CGCGCATATA TCCAAGCCAT GCGGGGAAAG TGTTTACTCC AGTTGAGTGG TCCAGCCGAT 
CCAACGGGAT GTGGAGAGGG ATTTTCATAT GTTCGAGTGC CAAACAAGCC CACGCAAACC 
AAGGAGGAGC AAGAGTCGCA GCCTAAACGT TCGGTCACAG GAACAGATGC AGATTTGCGT 
CGTCTGCCAC TCCAGCGTGC AAAAGAGCTG TTGCGGCAGT TCAAGGTGCC CGAGGAGGAG 
ATCAAAAAGC TTTCCCGCTG GGAGGTCATT GACGTGGTGC GCACCCTGTC CACAGAAAAG 
GCCAAGGCCG GTGAAGAGGG AATGGATAAG TTTTCTCGTG GCAACCGGTT CTCCATTGCA 
GAGCATCAGG AGCGTTATAA GGAAGAGTGC CAGCGCATAT TCGATCTGCA AAACAGAGTG 
CTGGCCAGCT CTGAGGTGCT GTCCACAGAT GAGGCAGAGT CCTCGGCCTC TGAGGAATCT 
GATCTCGAAG AACTTGGCAA GAATCTTGAG AACATGCTGT CAAACAAGAA AACCTCGACG 
CAATTGTCAA GGGAACGTGA AGAGCTGGAG CGTCAGGAGT TGCTTCGCCA GCTTGACGAA 
GAACACGGCG GACCAAGTGG TAGTGGAGGA GCCAAGGGAG CCAAAGGAAA GGATGATCCG 
GGACAGCAAA TGCTGGCAAC CAACAACCAG GGCAGGATCC TTCGCATTAC GCGTACCTTT 
AGAGGTAACG ATGGCAAGGA ATATACTCGC GTGGAGACTG TGCGGCGGCA ACCAGTTATC 
GACGCCTACA TCAAGATTCG CACCACTAAG GACGAGCAGT TCATCAAGCA GTTCGCAACG 
CTAGATGAGC AGCAGAAGGA GGAGATGAAG CGCGAAAAGA GACGCATTCA GGAGCAGCTA 
CGTCGCATCA AGCGCAACCA GGAGCGCGAA CGCCTGGCGC AGCTGGCCCA GAACCAGAAG 
CTTCAGCCAG GTGGCATGCC CACTTCCTTG GGTGATCCTA AGAGCTCGGG CGGTCATTCG 
CACAAGGAGC GGGATAGCGG CTACAAGGAG GTCAGCCCTT CGCGCAAGAA GTTCAAGCTT 
AAGCCAGACC TAAAGCTGAA GTGCGGCGCC TGTGGACAGG TTGGTCACAT GCGCACAAAC 
AAAGCCTGTC CCTTGTATTC TGGCATGCAA AGCAGTCTGT CCCAGTCGAA CCCATCTCTG 
GCTGACGATT TTGACGAACA GAGCGAAAAG GAGATGACAA TGGATGACGA TGATCTTGTG 
AATGTCGATG GCACCAAAGT AACGCTCAGC AGTAAGATTC TCAAGCGTCA TGGTGGTGAT 
GATGGCAAGC GTCGCAGCGG ATCTAGCTCT GGTTTCACCT TGAAGGTTCC CCGAGATGCG 
ATGGGCAAGA AGAAACGCAG AGTGGGTGGC GATCTTCATT GTGACTATCT GCAGCGACAC 
AATAAAACGG CCAATCGCAG GCGCACGGAC CCCGTTGTGG TACTGTCCTC TATCCTGGAG 
ATTATCCATA ATGAGCTGCG ATCTATGCCA GATGTATCGC CATTCCTGTT CCCGGTAAGC 
GCAAAAAAGG TTCCCGACTA CTACCGCGTG GTGACCAAGC CCATGGATCT GCAAACGATG 
AGGGAGTATA TTCGCCAAAG GCGCTACACG AGTCGCGAGA TGTTCCTCGA GGATCTCAAG 
CAGATTGTGG ACAACTCGCT GATCTACAAT GGACCGCAGA GTGCATACAC CTTGGCTGCC 
CAACGCATGT TCAGCAGTTG TTTTGAATTG CTCGCAGAGC GCGAAGACAA ACTGATGCGC 
CTCGAGAAGG CAATTAACCC GCTGCTGGAC GACGATGACC AAGTGGCACT CTCCTTTATC 
TTTGACAAGC TGCACTCGCA GATTAAGCAA TTACCAGAGA GCTGGCCTTT CCTTAAGCCT 
GTCAACAAGA AACAGGTTAA GGACTACTAC ACGGTTATCA AGCGACCCAT GGACCTCGAA 
ACTATCGGCA AAAACATTGA AGCTCATCGC TATCACAGTC GTGCCGAGTA TCTGGCTGAT 
ATCGAGTTGA TCGCCACCAA CTGTGAGCAG TACAACGGCA GTGACACCCG CTACACCAAG 
TTCTCAAAGA AGATACTTGA GTATGCCCAA ACCCAGTTAA TTGAGTTTTC GGAGCACTGC 
GGCCAGTTGG AAAATAACAT AGCTAAGACG CAGGAGCGTG CTAGGGAAAA TGCACCAGAG 
TTTGATGAAG CCTGGGGCAA TGATGATTAC AACTTTGACC GTGGCAGTAG GGCCAGTTCA 
CCCGGAGATG ACTACATCGA CGTCGAGGGT CATGGGGGGC ATGCCTCCTC ATCGAACTCT 
ATCCATCGCA GCATGGGCGC CGAGGCCGGT TCGTCACATA CGGCGCCGGC GGTGCGAAAA 
CCAGCTCCTC CTGGTCCTGG TGAGGTGAAG CGCGGAAGGG GTAGGCCCCG CAAGCAGCGC 
GACCCCGTGG AGGAGGATCT CCAATGCTCC ACAGATGACG AGGACGACGA CGAGGAGGAG 
GACTTCCAGG AGGTCTCCGA AGACGAGAAC AATGCGGCGA GCATTTTAGA TCAGGGCGAA 
CGTATCAATG CGCCTGCCGA TGCCATGGAT GGCATGTTTG ACCCCAAGAA CATCAAGACA 
GAGATTGACC TAGAGGCTCA CCAGATGGCA GAGGAGCCGA TCGGCGAGGA TGACAGCCAG 
CAGGTGGCCG AAGCAATGGT GCAGTTGAGT GGCGTGGGCG GCTACTATGC TCAACAGCAG 
CAAGATGAAT CCATGGATGT GGACCCCAAC TACGATCCCT CAGATTTCCT CGCCATGCAC 
AAGCAGCGCC AGAGCCTCGG CGAGCCCAGC AGCTTGCAGG GTGCTTTCAC CAACTTCCTA 
TCGCACGAGC AGGATGATAA TGGGCCTTAC AATCCCGCCG AAGCCAGCAC AAGTGCCGCT 
TCCGGTGCAG ACTTAGGAAT GGACGCTTCA ATGGCCATGC AAATGGCGCC GGAAATGCCT 
GTCAATACCA TGAACAACGG AATGGGCATC GATGATGATC TGGATATTTC GGAGAGTGAC 
GAGGAAGACG ATGGTTCTCG AGTGCGTATC AAAAAGGAGG TCTTCGACGA CGGGGATTAC 
GCCTTGCAGC ACCAGCAGAT GGGACAGGCA GCATCGCAGT CGCAGATATA CATGGTGGAT 
TCGTCCAACG AGCCCACGAC TCTCGACTAC CAGCAACCAC CGCAACTGGA CTTCCAACAA 
GTGCAGGAAA TGGAGCAGTT GCAGCACCAA GTGATGCCAC CAATGCAATC AGAGCAACTG 
CAGCAGCAAC AGACGCCGCA GGGAGACAAT GATTATGCCT GGACTTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163532.1
CDS	102..6392
Translation	MEMESDNSDDEGSIGNGLDLTGILFGNIDSEGRLLQDDDGEGRGGTGFDAELRENIGSLSKLGLDSMLLEVIDLKEAEPPSDDEEEEDARPSAVSASEGMSAFDALKAGVKREDGAVKAQDDAIDYSDITELSEDCPRTPPEETSTYDDLEDAIPASKVEAKLTKDDKELMPPPSAPMRSGSGGGIEEPAKSNDASSPSDDSKSTDSKDADRKLDTPLADILPSKYQNVDVRELFPDFRPQKVLRFSRLFGPGKPTSLPQIWRHVRKRRRKRNQSRDQKTTNTGGSDSPSDTEEPRKRGFSLHYAAEPTPAECMSDDEDKLLGDFNSEDVRPEGPDNGENSDQKPKVADWRFGPAQIWYDMLEVPDSGEGFNYGFKTKAASTSSQPQLKDERRVKSPEDDVEDPSIADDAFLMVSQLHWEDDVVWDGNDIKAKVLQKLNSKTNAAGWLPSSGSRTAGAFSQPGKPSMPVGSGSSKQGSGASSKKAQQNAQAKPAEAPDDTWYSLFPVENEELIYYKWEDEVIWDAQQVSKVPKPKVLTLDPNDENIILGIPDDIDPSKINKSTGPPPKIKIPHPHVKKSKILLGKAGVINVLAEDTPPPPPKSPDRDPFNISNDTYYTPKTEPTLRLKVGGNLIQHSTPVVELRAPFVPTHMGPMKLRAFHRPPLKKYSHGPMAQSIPHPVFPLLKTIAKKAKQREVERIASGGGDVFFMRNPEDLSGRDGDIVLAEFCEEHPPLINQVGMCSKIKNYYKRKAEKDSGPQDFVYGEVAFAHTSPFLGILHPGQCIQAIENNMYRAPIYPHKMAHNDFLVIRTRNNYWIRSVNSIYTVGQECPLYEVPGPNSKRANNFTRDFLQVFIYRLFWKSRDNPRRIRMDDIKQAFPAHSESSIRKRLKQCADFKRTGMDSNWWVIKPEFRLPSEEEIRAMVSPEQCCAYFSMIAAEQRLKDAGYGEKFLFAPQEDDDEEAQLKLDDEVKVAPWNTTRAYIQAMRGKCLLQLSGPADPTGCGEGFSYVRVPNKPTQTKEEQESQPKRSVTGTDADLRRLPLQRAKELLRQFKVPEEEIKKLSRWEVIDVVRTLSTEKAKAGEEGMDKFSRGNRFSIAEHQERYKEECQRIFDLQNRVLASSEVLSTDEAESSASEESDLEELGKNLENMLSNKKTSTQLSREREELERQELLRQLDEEHGGPSGSGGAKGAKGKDDPGQQMLATNNQGRILRITRTFRGNDGKEYTRVETVRRQPVIDAYIKIRTTKDEQFIKQFATLDEQQKEEMKREKRRIQEQLRRIKRNQERERLAQLAQNQKLQPGGMPTSLGDPKSSGGHSHKERDSGYKEVSPSRKKFKLKPDLKLKCGACGQVGHMRTNKACPLYSGMQSSLSQSNPSLADDFDEQSEKEMTMDDDDLVNVDGTKVTLSSKILKRHGGDDGKRRSGSSSGFTLKVPRDAMGKKKRRVGGDLHCDYLQRHNKTANRRRTDPVVVLSSILEIIHNELRSMPDVSPFLFPVSAKKVPDYYRVVTKPMDLQTMREYIRQRRYTSREMFLEDLKQIVDNSLIYNGPQSAYTLAAQRMFSSCFELLAEREDKLMRLEKAINPLLDDDDQVALSFIFDKLHSQIKQLPESWPFLKPVNKKQVKDYYTVIKRPMDLETIGKNIEAHRYHSRAEYLADIELIATNCEQYNGSDTRYTKFSKKILEYAQTQLIEFSEHCGQLENNIAKTQERARENAPEFDEAWGNDDYNFDRGSRASSPGDDYIDVEGHGGHASSSNSIHRSMGAEAGSSHTAPAVRKPAPPGPGEVKRGRGRPRKQRDPVEEDLQCSTDDEDDDEEEDFQEVSEDENNAASILDQGERINAPADAMDGMFDPKNIKTEIDLEAHQMAEEPIGEDDSQQVAEAMVQLSGVGGYYAQQQQDESMDVDPNYDPSDFLAMHKQRQSLGEPSSLQGAFTNFLSHEQDDNGPYNPAEASTSAASGADLGMDASMAMQMAPEMPVNTMNNGMGIDDDLDISESDEEDDGSRVRIKKEVFDDGDYALQHQQMGQAASQSQIYMVDSSNEPTTLDYQQPPQLDFQQVQEMEQLQHQVMPPMQSEQLQQQQTPQGDNDYAWTF

Target ORF information:

RefSeq Version	NM_001170061.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170061.1

ORF Insert Sequence:

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA CTAAGGACGA CAAGGAACTA ATGCCTCCAC CAAGTGCACC AATGCGCTCC 
GGCTCTGGCG GCGGCATCGA GGAACCGGCC AAGTCAAATG ATGCATCTAG TCCCAGTGAC 
GATTCCAAAT CTACCGATTC AAAGGATGCG GATCGGAAGC TGGACACACC ACTGGCAGAC 
ATACTGCCGT CCAAGTACCA GAATGTGGAT GTGCGTGAGC TCTTTCCGGA CTTTCGTCCC 
CAAAAGGTGC TACGCTTCTC CCGTCTCTTC GGACCCGGTA AACCCACGAG TTTGCCCCAG 
ATCTGGCGAC ACGTGCGCAA ACGCCGCCGC AAGCGAAATC AATCCAGGGA TCAGAAGACG 
ACAAACACTG GTGGTTCGGA CTCTCCAAGC GATACTGAGG AGCCACGAAA GCGGGGCTTC 
AGTCTGCACT ATGCTGCAGA GCCAACGCCA GCGGAGTGCA TGTCCGACGA CGAGGACAAA 
TTGCTGGGCG ACTTCAACAG CGAGGATGTG CGTCCAGAAG GACCGGACAA CGGCGAGAAC 
AGCGATCAGA AGCCAAAGGT GGCTGACTGG CGGTTCGGGC CTGCACAGAT TTGGTATGAT 
ATGCTAGAGG TGCCCGACTC TGGAGAGGGG TTCAACTATG GCTTCAAGAC AAAGGCAGCA 
AGCACCTCGT CTCAGCCGCA ACTCAAAGAT GAACGCCGTG TAAAGAGTCC AGAAGATGAT 
GTCGAAGATC CAAGCATTGC GGATGATGCC TTTCTCATGG TCTCCCAGCT GCATTGGGAA 
GACGACGTGG TCTGGGACGG TAACGACATC AAGGCAAAGG TGCTCCAAAA GCTTAACTCA 
AAGACAAATG CAGCCGGATG GTTGCCCTCG AGCGGGTCGA GAACGGCTGG CGCCTTTAGC 
CAGCCGGGCA AACCGTCTAT GCCCGTGGGA AGTGGAAGCT CCAAGCAGGG TTCGGGAGCA 
TCAAGCAAAA AGGCCCAGCA AAATGCTCAA GCAAAGCCAG CGGAGGCACC TGATGACACC 
TGGTATAGCC TTTTTCCAGT TGAAAATGAG GAGCTAATAT ACTACAAGTG GGAGGACGAG 
GTAATCTGGG ATGCCCAGCA AGTGAGCAAG GTGCCCAAGC CGAAGGTACT CACTTTGGAT 
CCCAACGACG AAAACATCAT TTTGGGTATT CCTGATGACA TAGATCCCTC TAAAATCAAT 
AAGAGCACGG GTCCTCCACC TAAGATCAAG ATACCGCATC CTCACGTAAA AAAGTCTAAG 
ATCCTACTTG GCAAGGCGGG TGTGATCAAC GTGCTTGCGG AGGACACACC TCCGCCGCCA 
CCCAAAAGTC CTGACCGTGA CCCCTTTAAC ATATCCAATG ACACTTACTA CACACCGAAG 
ACAGAACCCA CCCTACGACT TAAGGTGGGC GGTAATCTTA TTCAACACTC GACTCCGGTG 
GTAGAGCTGC GAGCTCCGTT TGTGCCTACG CACATGGGCC CGATGAAGCT CCGCGCCTTC 
CATCGCCCCC CCCTAAAGAA GTACTCCCAC GGGCCAATGG CCCAGTCTAT CCCTCATCCT 
GTCTTCCCAC TGCTGAAGAC CATCGCAAAG AAGGCGAAGC AGCGCGAAGT GGAGCGCATT 
GCCTCCGGTG GCGGAGACGT CTTCTTTATG CGCAACCCAG AGGATTTAAG CGGAAGGGAC 
GGAGACATCG TACTAGCCGA GTTCTGCGAA GAGCATCCGC CTCTGATAAA CCAGGTGGGC 
ATGTGCTCCA AGATAAAGAA CTACTACAAG CGTAAGGCTG AGAAGGACAG TGGGCCGCAG 
GATTTTGTTT ATGGAGAAGT GGCCTTTGCG CACACAAGTC CCTTCCTGGG CATCCTGCAT 
CCTGGCCAGT GCATACAGGC GATTGAGAAC AACATGTACC GAGCGCCTAT TTATCCACAT 
AAGATGGCTC ACAACGATTT CCTCGTCATT CGCACGCGGA ACAACTACTG GATACGGTCG 
GTGAATTCAA TATACACCGT GGGTCAGGAG TGTCCGCTGT ACGAGGTACC CGGTCCGAAT 
TCCAAAAGGG CCAACAACTT CACCCGTGAC TTTCTGCAGG TGTTTATTTA CCGCCTGTTC 
TGGAAAAGTC GCGACAACCC GCGCCGCATT CGAATGGACG ATATAAAACA GGCTTTTCCC 
GCTCATTCCG AGAGCAGCAT CCGCAAGCGT TTAAAGCAGT GCGCTGACTT CAAGCGAACA 
GGCATGGACT CCAATTGGTG GGTTATAAAG CCAGAGTTTC GCCTTCCATC CGAGGAGGAG 
ATCCGAGCCA TGGTGTCACC TGAGCAGTGT TGCGCGTACT TCAGCATGAT AGCGGCGGAA 
CAACGCTTAA AGGATGCTGG GTATGGAGAA AAGTTTTTGT TCGCACCTCA GGAAGATGAC 
GACGAGGAGG CGCAGCTGAA GCTTGACGAC GAAGTAAAGG TGGCTCCTTG GAACACGACT 
CGCGCATATA TCCAAGCCAT GCGGGGAAAG TGTTTACTCC AGTTGAGTGG TCCAGCCGAT 
CCAACGGGAT GTGGAGAGGG ATTTTCATAT GTTCGAGTGC CAAACAAGCC CACGCAAACC 
AAGGAGGAGC AAGAGTCGCA GCCTAAACGT TCGGTCACAG GAACAGATGC AGATTTGCGT 
CGTCTGCCAC TCCAGCGTGC AAAAGAGCTG TTGCGGCAGT TCAAGGTGCC CGAGGAGGAG 
ATCAAAAAGC TTTCCCGCTG GGAGGTCATT GACGTGGTGC GCACCCTGTC CACAGAAAAG 
GCCAAGGCCG GTGAAGAGGG AATGGATAAG TTTTCTCGTG GCAACCGGTT CTCCATTGCA 
GAGCATCAGG AGCGTTATAA GGAAGAGTGC CAGCGCATAT TCGATCTGCA AAACAGAGTG 
CTGGCCAGCT CTGAGGTGCT GTCCACAGAT GAGGCAGAGT CCTCGGCCTC TGAGGAATCT 
GATCTCGAAG AACTTGGCAA GAATCTTGAG AACATGCTGT CAAACAAGAA AACCTCGACG 
CAATTGTCAA GGGAACGTGA AGAGCTGGAG CGTCAGGAGT TGCTTCGCCA GCTTGACGAA 
GAACACGGCG GACCAAGTGG TAGTGGAGGA GCCAAGGGAG CCAAAGGAAA GGATGATCCG 
GGACAGCAAA TGCTGGCAAC CAACAACCAG GGCAGGATCC TTCGCATTAC GCGTACCTTT 
AGAGGTAACG ATGGCAAGGA ATATACTCGC GTGGAGACTG TGCGGCGGCA ACCAGTTATC 
GACGCCTACA TCAAGATTCG CACCACTAAG GACGAGCAGT TCATCAAGCA GTTCGCAACG 
CTAGATGAGC AGCAGAAGGA GGAGATGAAG CGCGAAAAGA GACGCATTCA GGAGCAGCTA 
CGTCGCATCA AGCGCAACCA GGAGCGCGAA CGCCTGGCGC AGCTGGCCCA GAACCAGAAG 
CTTCAGCCAG GTGGCATGCC CACTTCCTTG GGTGATCCTA AGAGCTCGGG CGGTCATTCG 
CACAAGGAGC GGGATAGCGG CTACAAGGAG GTCAGCCCTT CGCGCAAGAA GTTCAAGCTT 
AAGCCAGACC TAAAGCTGAA GTGCGGCGCC TGTGGACAGG TTGGTCACAT GCGCACAAAC 
AAAGCCTGTC CCTTGTATTC TGGCATGCAA AGCAGTCTGT CCCAGTCGAA CCCATCTCTG 
GCTGACGATT TTGACGAACA GAGCGAAAAG GAGATGACAA TGGATGACGA TGATCTTGTG 
AATGTCGATG GCACCAAAGT AACGCTCAGC AGTAAGATTC TCAAGCGTCA TGGTGGTGAT 
GATGGCAAGC GTCGCAGCGG ATCTAGCTCT GGTTTCACCT TGAAGGTTCC CCGAGATGCG 
ATGGGCAAGA AGAAACGCAG AGTGGGTGGC GATCTTCATT GTGACTATCT GCAGCGACAC 
AATAAAACGG CCAATCGCAG GCGCACGGAC CCCGTTGTGG TACTGTCCTC TATCCTGGAG 
ATTATCCATA ATGAGCTGCG ATCTATGCCA GATGTATCGC CATTCCTGTT CCCGGTAAGC 
GCAAAAAAGG TTCCCGACTA CTACCGCGTG GTGACCAAGC CCATGGATCT GCAAACGATG 
AGGGAGTATA TTCGCCAAAG GCGCTACACG AGTCGCGAGA TGTTCCTCGA GGATCTCAAG 
CAGATTGTGG ACAACTCGCT GATCTACAAT GGACCGCAGA GTGCATACAC CTTGGCTGCC 
CAACGCATGT TCAGCAGTTG TTTTGAATTG CTCGCAGAGC GCGAAGACAA ACTGATGCGC 
CTCGAGAAGG CAATTAACCC GCTGCTGGAC GACGATGACC AAGTGGCACT CTCCTTTATC 
TTTGACAAGC TGCACTCGCA GATTAAGCAA TTACCAGAGA GCTGGCCTTT CCTTAAGCCT 
GTCAACAAGA AACAGGTTAA GGACTACTAC ACGGTTATCA AGCGACCCAT GGACCTCGAA 
ACTATCGGCA AAAACATTGA AGCTCATCGC TATCACAGTC GTGCCGAGTA TCTGGCTGAT 
ATCGAGTTGA TCGCCACCAA CTGTGAGCAG TACAACGGCA GTGACACCCG CTACACCAAG 
TTCTCAAAGA AGATACTTGA GTATGCCCAA ACCCAGTTAA TTGAGTTTTC GGAGCACTGC 
GGCCAGTTGG AAAATAACAT AGCTAAGACG CAGGAGCGTG CTAGGGAAAA TGCACCAGAG 
TTTGATGAAG CCTGGGGCAA TGATGATTAC AACTTTGACC GTGGCAGTAG GGCCAGTTCA 
CCCGGAGATG ACTACATCGA CGTCGAGGGT CATGGGGGGC ATGCCTCCTC ATCGAACTCT 
ATCCATCGCA GCATGGGCGC CGAGGCCGGT TCGTCACATA CGGCGCCGGC GGTGCGAAAA 
CCAGCTCCTC CTGGTCCTGG TGAGGTGAAG CGCGGAAGGG GTAGGCCCCG CAAGCAGCGC 
GACCCCGTGG AGGAGGATCT CCAATGCTCC ACAGATGACG AGGACGACGA CGAGGAGGAG 
GACTTCCAGG AGGTCTCCGA AGACGAGAAC AATGCGGCGA GCATTTTAGA TCAGGGCGAA 
CGTATCAATG CGCCTGCCGA TGCCATGGAT GGCATGTTTG ACCCCAAGAA CATCAAGACA 
GAGATTGACC TAGAGGCTCA CCAGATGGCA GAGGAGCCGA TCGGCGAGGA TGACAGCCAG 
CAGGTGGCCG AAGCAATGGT GCAGTTGAGT GGCGTGGGCG GCTACTATGC TCAACAGCAG 
CAAGATGAAT CCATGGATGT GGACCCCAAC TACGATCCCT CAGATTTCCT CGCCATGCAC 
AAGCAGCGCC AGAGCCTCGG CGAGCCCAGC AGCTTGCAGG GTGCTTTCAC CAACTTCCTA 
TCGCACGAGC AGGATGATAA TGGGCCTTAC AATCCCGCCG AAGCCAGCAC AAGTGCCGCT 
TCCGGTGCAG ACTTAGGAAT GGACGCTTCA ATGGCCATGC AAATGGCGCC GGAAATGCCT 
GTCAATACCA TGAACAACGG AATGGGCATC GATGATGATC TGGATATTTC GGAGAGTGAC 
GAGGAAGACG ATGGTTCTCG AGTGCGTATC AAAAAGGAGG TCTTCGACGA CGGGGATTAC 
GCCTTGCAGC ACCAGCAGAT GGGACAGGCA GCATCGCAGT CGCAGATATA CATGGTGGAT 
TCGTCCAACG AGCCCACGAC TCTCGACTAC CAGCAACCAC CGCAACTGGA CTTCCAACAA 
GTGCAGGAAA TGGAGCAGTT GCAGCACCAA GTGATGCCAC CAATGCAATC AGAGCAACTG 
CAGCAGCAAC AGACGCCGCA GGGAGACAAT GATTATGCCT GGACTTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Taf1 ( NM_001300272.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287201.1 Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant F, mRNA.

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CloneID	OFa22059
Clone ID Related Accession (Same CDS sequence)	NM_001300272.1
Accession Version	NM_001300272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TBP-associated factor 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA CTAAGGACGA CAAGGAACTA ATGCCTCCAC CAAGTGCACC AATGCGCTCC 
GGCTCTGGCG GCGGCATCGA GGAACCGGCC AAGTCAAATG ATGCATCTAG TCCCAGTGAC 
GATTCCAAAT CTACCGATTC AAAGGATGCG GATCGGAAGC TGGACACACC ACTGGCAGAC 
ATACTGCCGT CCAAGTACCA GAATGTGGAT GTGCGTGAGC TCTTTCCGGA CTTTCGTCCC 
CAAAAGGTGC TACGCTTCTC CCGTCTCTTC GGACCCGGTA AACCCACGAG TTTGCCCCAG 
ATCTGGCGAC ACGTGCGCAA ACGCCGCCGC AAGCGAAATC AATCCAGGGA TCAGAAGACG 
ACAAACACTG GTGGTTCGGA CTCTCCAAGC GATACTGAGG AGCCACGAAA GCGGGGCTTC 
AGTCTGCACT ATGCTGCAGA GCCAACGCCA GCGGAGTGCA TGTCCGACGA CGAGGACAAA 
TTGCTGGGCG ACTTCAACAG CGAGGATGTG CGTCCAGAAG GACCGGACAA CGGCGAGAAC 
AGCGATCAGA AGCCAAAGGT GGCTGACTGG CGGTTCGGGC CTGCACAGAT TTGGTATGAT 
ATGCTAGAGG TGCCCGACTC TGGAGAGGGG TTCAACTATG GCTTCAAGAC AAAGGCAGCA 
AGCACCTCGT CTCAGCCGCA ACTCAAAGAT GAACGCCGTG TAAAGAGTCC AGAAGATGAT 
GTCGAAGATC CAAGCATTGC GGATGATGCC TTTCTCATGG TCTCCCAGCT GCATTGGGAA 
GACGACGTGG TCTGGGACGG TAACGACATC AAGGCAAAGG TGCTCCAAAA GCTTAACTCA 
AAGACAAATG CAGCCGGATG GTTGCCCTCG AGCGGGTCGA GAACGGCTGG CGCCTTTAGC 
CAGCCGGGCA AACCGTCTAT GCCCGTGGGA AGTGGAAGCT CCAAGCAGGG TTCGGGAGCA 
TCAAGCAAAA AGGCCCAGCA AAATGCTCAA GCAAAGCCAG CGGAGGCACC TGATGACACC 
TGGTATAGCC TTTTTCCAGT TGAAAATGAG GAGCTAATAT ACTACAAGTG GGAGGACGAG 
GTAATCTGGG ATGCCCAGCA AGTGAGCAAG GTGCCCAAGC CGAAGGTACT CACTTTGGAT 
CCCAACGACG AAAACATCAT TTTGGGTATT CCTGATGACA TAGATCCCTC TAAAATCAAT 
AAGAGCACGG GTCCTCCACC TAAGATCAAG ATACCGCATC CTCACGTAAA AAAGTCTAAG 
ATCCTACTTG GCAAGGCGGG TGTGATCAAC GTGCTTGCGG AGGACACACC TCCGCCGCCA 
CCCAAAAGTC CTGACCGTGA CCCCTTTAAC ATATCCAATG ACACTTACTA CACACCGAAG 
ACAGAACCCA CCCTACGACT TAAGGTGGGC GGTAATCTTA TTCAACACTC GACTCCGGTG 
GTAGAGCTGC GAGCTCCGTT TGTGCCTACG CACATGGGCC CGATGAAGCT CCGCGCCTTC 
CATCGCCCCC CCCTAAAGAA GTACTCCCAC GGGCCAATGG CCCAGTCTAT CCCTCATCCT 
GTCTTCCCAC TGCTGAAGAC CATCGCAAAG AAGGCGAAGC AGCGCGAAGT GGAGCGCATT 
GCCTCCGGTG GCGGAGACGT CTTCTTTATG CGCAACCCAG AGGATTTAAG CGGAAGGGAC 
GGAGACATCG TACTAGCCGA GTTCTGCGAA GAGCATCCGC CTCTGATAAA CCAGGTGGGC 
ATGTGCTCCA AGATAAAGAA CTACTACAAG CGTAAGGCTG AGAAGGACAG TGGGCCGCAG 
GATTTTGTTT ATGGAGAAGT GGCCTTTGCG CACACAAGTC CCTTCCTGGG CATCCTGCAT 
CCTGGCCAGT GCATACAGGC GATTGAGAAC AACATGTACC GAGCGCCTAT TTATCCACAT 
AAGATGGCTC ACAACGATTT CCTCGTCATT CGCACGCGGA ACAACTACTG GATACGGTCG 
GTGAATTCAA TATACACCGT GGGTCAGGAG TGTCCGCTGT ACGAGGTACC CGGTCCGAAT 
TCCAAAAGGG CCAACAACTT CACCCGTGAC TTTCTGCAGG TGTTTATTTA CCGCCTGTTC 
TGGAAAAGTC GCGACAACCC GCGCCGCATT CGAATGGACG ATATAAAACA GGCTTTTCCC 
GCTCATTCCG AGAGCAGCAT CCGCAAGCGT TTAAAGCAGT GCGCTGACTT CAAGCGAACA 
GGCATGGACT CCAATTGGTG GGTTATAAAG CCAGAGTTTC GCCTTCCATC CGAGGAGGAG 
ATCCGAGCCA TGGTGTCACC TGAGCAGTGT TGCGCGTACT TCAGCATGAT AGCGGCGGAA 
CAACGCTTAA AGGATGCTGG GTATGGAGAA AAGTTTTTGT TCGCACCTCA GGAAGATGAC 
GACGAGGAGG CGCAGCTGAA GCTTGACGAC GAAGTAAAGG TGGCTCCTTG GAACACGACT 
CGCGCATATA TCCAAGCCAT GCGGGGAAAG TGTTTACTCC AGTTGAGTGG TCCAGCCGAT 
CCAACGGGAT GTGGAGAGGG ATTTTCATAT GTTCGAGTGC CAAACAAGCC CACGCAAACC 
AAGGAGGAGC AAGAGTCGCA GCCTAAACGT TCGGTCACAG GAACAGATGC AGATTTGCGT 
CGTCTGCCAC TCCAGCGTGC AAAAGAGCTG TTGCGGCAGT TCAAGGTGCC CGAGGAGGAG 
ATCAAAAAGC TTTCCCGCTG GGAGGTCATT GACGTGGTGC GCACCCTGTC CACAGAAAAG 
GCCAAGGCCG GTGAAGAGGG AATGGATAAG TTTTCTCGTG GCAACCGGTT CTCCATTGCA 
GAGCATCAGG AGCGTTATAA GGAAGAGTGC CAGCGCATAT TCGATCTGCA AAACAGAGTG 
CTGGCCAGCT CTGAGGTGCT GTCCACAGAT GAGGCAGAGT CCTCGGCCTC TGAGGAATCT 
GATCTCGAAG AACTTGGCAA GAATCTTGAG AACATGCTGT CAAACAAGAA AACCTCGACG 
CAATTGTCAA GGGAACGTGA AGAGCTGGAG CGTCAGGAGT TGCTTCGCCA GCTTGACGAA 
GAACACGGCG GACCAAGTGG TAGTGGAGGA GCCAAGGGAG CCAAAGGAAA GGATGATCCG 
GGACAGCAAA TGCTGGCAAC CAACAACCAG GGCAGGATCC TTCGCATTAC GCGTACCTTT 
AGAGGTAACG ATGGCAAGGA ATATACTCGC GTGGAGACTG TGCGGCGGCA ACCAGTTATC 
GACGCCTACA TCAAGATTCG CACCACTAAG GACGAGCAGT TCATCAAGCA GTTCGCAACG 
CTAGATGAGC AGCAGAAGGA GGAGATGAAG CGCGAAAAGA GACGCATTCA GGAGCAGCTA 
CGTCGCATCA AGCGCAACCA GGAGCGCGAA CGCCTGGCGC AGCTGGCCCA GAACCAGAAG 
CTTCAGCCAG GTGGCATGCC CACTTCCTTG GGTGATCCTA AGAGCTCGGG CGGTCATTCG 
CACAAGGAGC GGGATAGCGG CTACAAGGAG GTCAGCCCTT CGCGCAAGAA GTTCAAGCTT 
AAGCCAGACC TAAAGCTGAA GTGCGGCGCC TGTGGACAGG TTGGTCACAT GCGCACAAAC 
AAAGCCTGTC CCTTGTATTC TGGCATGCAA AGCAGTCTGT CCCAGTCGAA CCCATCTCTG 
GCTGACGATT TTGACGAACA GAGCGAAAAG GAGATGACAA TGGATGACGA TGATCTTGTG 
AATGTCGATG GCACCAAAGT AACGCTCAGC AGTAAGATTC TCAAGCGTCA TGGTGGTGAT 
GATGGCAAGC GTCGCAGCGG ATCTAGCTCT GGTTTCACCT TGAAGGTTCC CCGAGATGCG 
ATGGGCAAGA AGAAACGCAG AGTGGGTGGC GATCTTCATT GTGACTATCT GCAGCGACAC 
AATAAAACGG CCAATCGCAG GCGCACGGAC CCCGTTGTGG TACTGTCCTC TATCCTGGAG 
ATTATCCATA ATGAGCTGCG ATCTATGCCA GATGTATCGC CATTCCTGTT CCCGGTAAGC 
GCAAAAAAGG TTCCCGACTA CTACCGCGTG GTGACCAAGC CCATGGATCT GCAAACGATG 
AGGGAGTATA TTCGCCAAAG GCGCTACACG AGTCGCGAGA TGTTCCTCGA GGATCTCAAG 
CAGATTGTGG ACAACTCGCT GATCTACAAT GGACCGCAGA GTGCATACAC CTTGGCTGCC 
CAACGCATGT TCAGCAGTTG TTTTGAATTG CTCGCAGAGC GCGAAGACAA ACTGATGCGC 
CTCGAGAAGG CAATTAACCC GCTGCTGGAC GACGATGACC AAGTGGCACT CTCCTTTATC 
TTTGACAAGC TGCACTCGCA GATTAAGCAA TTACCAGAGA GCTGGCCTTT CCTTAAGCCT 
GTCAACAAGA AACAGGTTAA GGACTACTAC ACGGTTATCA AGCGACCCAT GGACCTCGAA 
ACTATCGGCA AAAACATTGA AGCTCATCGC TATCACAGTC GTGCCGAGTA TCTGGCTGAT 
ATCGAGTTGA TCGCCACCAA CTGTGAGCAG TACAACGGCA GTGACACCCG CTACACCAAG 
TTCTCAAAGA AGATACTTGA GTATGCCCAA ACCCAGTTAA TTGAGTTTTC GGAGCACTGC 
GGCCAGTTGG AAAATAACAT AGCTAAGACG CAGGAGCGTG CTAGGGAAAA TGCACCAGAG 
TTTGATGAAG CCTGGGGCAA TGATGATTAC AACTTTGACC GTGGCAGTAG GGCCAGTTCA 
CCCGGAGATG ACTACATCGA CGTCGAGGGT CATGGGGGGC ATGCCTCCTC ATCGAACTCT 
ATCCATCGCA GCATGGGCGC CGAGGCCGGT TCGTCACATA CGGCGCCGGC GGTGCGAAAA 
CCAGCTCCTC CTGGTCCTGG TGAGGTGAAG CGCGGAAGGG GTAGGCCCCG CAAGCAGCGC 
GACCCCGTGG AGGAGGATCT CCAATGCTCC ACAGATGACG AGGACGACGA CGAGGAGGAG 
GACTTCCAGG AGGTCTCCGA AGACGAGAAC AATGCGGCGA GCATTTTAGA TCAGGGCGAA 
CGTATCAATG CGCCTGCCGA TGCCATGGAT GGCATGTTTG ACCCCAAGAA CATCAAGACA 
GAGATTGACC TAGAGGCTCA CCAGATGGCA GCAGAGGAGC CGATCGGCGA GGATGACAGC 
CAGCAGGTGG CCGAAGCAAT GGTGCAGTTG AGTGGCGTGG GCGGCTACTA TGCTCAACAG 
CAGCAAGATG AATCCATGGA TGTGGACCCC AACTACGATC CCTCAGATTT CCTCGCCATG 
CACAAGCAGC GCCAGAGCCT CGGCGAGCCC AGCAGCTTGC AGGGTGCTTT CACCAACTTC 
CTATCGCACG AGCAGGATGA TAATGGGCCT TACAATCCCG CCGAAGCCAG CACAAGTGCC 
GCTTCCGGTG CAGACTTAGG AATGGACGCT TCAATGGCCA TGCAAATGGC GCCGGAAATG 
CCTGTCAATA CCATGAACAA CGGAATGGGC ATCGATGATG ATCTGGATAT TTCGGAGAGT 
GACGAGGAAG ACGATGGTTC TCGAGTGCGT ATCAAAAAGG AGGTCTTCGA CGACGGGGAT 
TACGCCTTGC AGCACCAGCA GATGGGACAG GCAGCATCGC AGTCGCAGAT ATACATGGTG 
GATTCGTCCA ACGAGCCCAC GACTCTCGAC TACCAGCAAC CACCGCAACT GGACTTCCAA 
CAAGTGCAGG AAATGGAGCA GTTGCAGCAC CAAGTGATGC CACCAATGCA ATCAGAGCAA 
CTGCAGCAGC AACAGACGCC GCAGGGAGAC AATGATTATG CCTGGACTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287201.1
CDS	102..6395
Translation	MEMESDNSDDEGSIGNGLDLTGILFGNIDSEGRLLQDDDGEGRGGTGFDAELRENIGSLSKLGLDSMLLEVIDLKEAEPPSDDEEEEDARPSAVSASEGMSAFDALKAGVKREDGAVKAQDDAIDYSDITELSEDCPRTPPEETSTYDDLEDAIPASKVEAKLTKDDKELMPPPSAPMRSGSGGGIEEPAKSNDASSPSDDSKSTDSKDADRKLDTPLADILPSKYQNVDVRELFPDFRPQKVLRFSRLFGPGKPTSLPQIWRHVRKRRRKRNQSRDQKTTNTGGSDSPSDTEEPRKRGFSLHYAAEPTPAECMSDDEDKLLGDFNSEDVRPEGPDNGENSDQKPKVADWRFGPAQIWYDMLEVPDSGEGFNYGFKTKAASTSSQPQLKDERRVKSPEDDVEDPSIADDAFLMVSQLHWEDDVVWDGNDIKAKVLQKLNSKTNAAGWLPSSGSRTAGAFSQPGKPSMPVGSGSSKQGSGASSKKAQQNAQAKPAEAPDDTWYSLFPVENEELIYYKWEDEVIWDAQQVSKVPKPKVLTLDPNDENIILGIPDDIDPSKINKSTGPPPKIKIPHPHVKKSKILLGKAGVINVLAEDTPPPPPKSPDRDPFNISNDTYYTPKTEPTLRLKVGGNLIQHSTPVVELRAPFVPTHMGPMKLRAFHRPPLKKYSHGPMAQSIPHPVFPLLKTIAKKAKQREVERIASGGGDVFFMRNPEDLSGRDGDIVLAEFCEEHPPLINQVGMCSKIKNYYKRKAEKDSGPQDFVYGEVAFAHTSPFLGILHPGQCIQAIENNMYRAPIYPHKMAHNDFLVIRTRNNYWIRSVNSIYTVGQECPLYEVPGPNSKRANNFTRDFLQVFIYRLFWKSRDNPRRIRMDDIKQAFPAHSESSIRKRLKQCADFKRTGMDSNWWVIKPEFRLPSEEEIRAMVSPEQCCAYFSMIAAEQRLKDAGYGEKFLFAPQEDDDEEAQLKLDDEVKVAPWNTTRAYIQAMRGKCLLQLSGPADPTGCGEGFSYVRVPNKPTQTKEEQESQPKRSVTGTDADLRRLPLQRAKELLRQFKVPEEEIKKLSRWEVIDVVRTLSTEKAKAGEEGMDKFSRGNRFSIAEHQERYKEECQRIFDLQNRVLASSEVLSTDEAESSASEESDLEELGKNLENMLSNKKTSTQLSREREELERQELLRQLDEEHGGPSGSGGAKGAKGKDDPGQQMLATNNQGRILRITRTFRGNDGKEYTRVETVRRQPVIDAYIKIRTTKDEQFIKQFATLDEQQKEEMKREKRRIQEQLRRIKRNQERERLAQLAQNQKLQPGGMPTSLGDPKSSGGHSHKERDSGYKEVSPSRKKFKLKPDLKLKCGACGQVGHMRTNKACPLYSGMQSSLSQSNPSLADDFDEQSEKEMTMDDDDLVNVDGTKVTLSSKILKRHGGDDGKRRSGSSSGFTLKVPRDAMGKKKRRVGGDLHCDYLQRHNKTANRRRTDPVVVLSSILEIIHNELRSMPDVSPFLFPVSAKKVPDYYRVVTKPMDLQTMREYIRQRRYTSREMFLEDLKQIVDNSLIYNGPQSAYTLAAQRMFSSCFELLAEREDKLMRLEKAINPLLDDDDQVALSFIFDKLHSQIKQLPESWPFLKPVNKKQVKDYYTVIKRPMDLETIGKNIEAHRYHSRAEYLADIELIATNCEQYNGSDTRYTKFSKKILEYAQTQLIEFSEHCGQLENNIAKTQERARENAPEFDEAWGNDDYNFDRGSRASSPGDDYIDVEGHGGHASSSNSIHRSMGAEAGSSHTAPAVRKPAPPGPGEVKRGRGRPRKQRDPVEEDLQCSTDDEDDDEEEDFQEVSEDENNAASILDQGERINAPADAMDGMFDPKNIKTEIDLEAHQMAAEEPIGEDDSQQVAEAMVQLSGVGGYYAQQQQDESMDVDPNYDPSDFLAMHKQRQSLGEPSSLQGAFTNFLSHEQDDNGPYNPAEASTSAASGADLGMDASMAMQMAPEMPVNTMNNGMGIDDDLDISESDEEDDGSRVRIKKEVFDDGDYALQHQQMGQAASQSQIYMVDSSNEPTTLDYQQPPQLDFQQVQEMEQLQHQVMPPMQSEQLQQQQTPQGDNDYAWTF

Target ORF information:

RefSeq Version	NM_001300272.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TBP-associated factor 1 (Taf1), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300272.1

ORF Insert Sequence:

ATGGAGATGG AATCTGACAA CAGCGACGAT GAGGGATCGA TCGGCAATGG ATTGGACTTG 
ACCGGCATTC TTTTCGGCAA CATCGACTCC GAGGGCAGAC TGCTGCAAGA TGACGACGGA 
GAGGGGCGCG GGGGCACCGG TTTCGATGCG GAGCTCCGGG AAAACATTGG TTCCCTTTCC 
AAATTGGGTC TGGATTCTAT GCTGCTCGAG GTTATCGATC TCAAGGAAGC CGAGCCTCCG 
TCGGATGACG AGGAAGAGGA GGACGCCAGA CCGTCAGCCG TCAGTGCCAG CGAAGGAATG 
AGTGCCTTTG ATGCGCTCAA AGCGGGCGTT AAAAGGGAGG ATGGCGCCGT AAAGGCTCAG 
GACGATGCCA TTGACTACTC TGACATCACC GAGTTATCCG AGGACTGCCC ACGCACTCCG 
CCCGAAGAAA CAAGCACTTA TGATGACTTA GAAGACGCCA TTCCCGCCTC CAAGGTAGAG 
GCCAAGTTGA CTAAGGACGA CAAGGAACTA ATGCCTCCAC CAAGTGCACC AATGCGCTCC 
GGCTCTGGCG GCGGCATCGA GGAACCGGCC AAGTCAAATG ATGCATCTAG TCCCAGTGAC 
GATTCCAAAT CTACCGATTC AAAGGATGCG GATCGGAAGC TGGACACACC ACTGGCAGAC 
ATACTGCCGT CCAAGTACCA GAATGTGGAT GTGCGTGAGC TCTTTCCGGA CTTTCGTCCC 
CAAAAGGTGC TACGCTTCTC CCGTCTCTTC GGACCCGGTA AACCCACGAG TTTGCCCCAG 
ATCTGGCGAC ACGTGCGCAA ACGCCGCCGC AAGCGAAATC AATCCAGGGA TCAGAAGACG 
ACAAACACTG GTGGTTCGGA CTCTCCAAGC GATACTGAGG AGCCACGAAA GCGGGGCTTC 
AGTCTGCACT ATGCTGCAGA GCCAACGCCA GCGGAGTGCA TGTCCGACGA CGAGGACAAA 
TTGCTGGGCG ACTTCAACAG CGAGGATGTG CGTCCAGAAG GACCGGACAA CGGCGAGAAC 
AGCGATCAGA AGCCAAAGGT GGCTGACTGG CGGTTCGGGC CTGCACAGAT TTGGTATGAT 
ATGCTAGAGG TGCCCGACTC TGGAGAGGGG TTCAACTATG GCTTCAAGAC AAAGGCAGCA 
AGCACCTCGT CTCAGCCGCA ACTCAAAGAT GAACGCCGTG TAAAGAGTCC AGAAGATGAT 
GTCGAAGATC CAAGCATTGC GGATGATGCC TTTCTCATGG TCTCCCAGCT GCATTGGGAA 
GACGACGTGG TCTGGGACGG TAACGACATC AAGGCAAAGG TGCTCCAAAA GCTTAACTCA 
AAGACAAATG CAGCCGGATG GTTGCCCTCG AGCGGGTCGA GAACGGCTGG CGCCTTTAGC 
CAGCCGGGCA AACCGTCTAT GCCCGTGGGA AGTGGAAGCT CCAAGCAGGG TTCGGGAGCA 
TCAAGCAAAA AGGCCCAGCA AAATGCTCAA GCAAAGCCAG CGGAGGCACC TGATGACACC 
TGGTATAGCC TTTTTCCAGT TGAAAATGAG GAGCTAATAT ACTACAAGTG GGAGGACGAG 
GTAATCTGGG ATGCCCAGCA AGTGAGCAAG GTGCCCAAGC CGAAGGTACT CACTTTGGAT 
CCCAACGACG AAAACATCAT TTTGGGTATT CCTGATGACA TAGATCCCTC TAAAATCAAT 
AAGAGCACGG GTCCTCCACC TAAGATCAAG ATACCGCATC CTCACGTAAA AAAGTCTAAG 
ATCCTACTTG GCAAGGCGGG TGTGATCAAC GTGCTTGCGG AGGACACACC TCCGCCGCCA 
CCCAAAAGTC CTGACCGTGA CCCCTTTAAC ATATCCAATG ACACTTACTA CACACCGAAG 
ACAGAACCCA CCCTACGACT TAAGGTGGGC GGTAATCTTA TTCAACACTC GACTCCGGTG 
GTAGAGCTGC GAGCTCCGTT TGTGCCTACG CACATGGGCC CGATGAAGCT CCGCGCCTTC 
CATCGCCCCC CCCTAAAGAA GTACTCCCAC GGGCCAATGG CCCAGTCTAT CCCTCATCCT 
GTCTTCCCAC TGCTGAAGAC CATCGCAAAG AAGGCGAAGC AGCGCGAAGT GGAGCGCATT 
GCCTCCGGTG GCGGAGACGT CTTCTTTATG CGCAACCCAG AGGATTTAAG CGGAAGGGAC 
GGAGACATCG TACTAGCCGA GTTCTGCGAA GAGCATCCGC CTCTGATAAA CCAGGTGGGC 
ATGTGCTCCA AGATAAAGAA CTACTACAAG CGTAAGGCTG AGAAGGACAG TGGGCCGCAG 
GATTTTGTTT ATGGAGAAGT GGCCTTTGCG CACACAAGTC CCTTCCTGGG CATCCTGCAT 
CCTGGCCAGT GCATACAGGC GATTGAGAAC AACATGTACC GAGCGCCTAT TTATCCACAT 
AAGATGGCTC ACAACGATTT CCTCGTCATT CGCACGCGGA ACAACTACTG GATACGGTCG 
GTGAATTCAA TATACACCGT GGGTCAGGAG TGTCCGCTGT ACGAGGTACC CGGTCCGAAT 
TCCAAAAGGG CCAACAACTT CACCCGTGAC TTTCTGCAGG TGTTTATTTA CCGCCTGTTC 
TGGAAAAGTC GCGACAACCC GCGCCGCATT CGAATGGACG ATATAAAACA GGCTTTTCCC 
GCTCATTCCG AGAGCAGCAT CCGCAAGCGT TTAAAGCAGT GCGCTGACTT CAAGCGAACA 
GGCATGGACT CCAATTGGTG GGTTATAAAG CCAGAGTTTC GCCTTCCATC CGAGGAGGAG 
ATCCGAGCCA TGGTGTCACC TGAGCAGTGT TGCGCGTACT TCAGCATGAT AGCGGCGGAA 
CAACGCTTAA AGGATGCTGG GTATGGAGAA AAGTTTTTGT TCGCACCTCA GGAAGATGAC 
GACGAGGAGG CGCAGCTGAA GCTTGACGAC GAAGTAAAGG TGGCTCCTTG GAACACGACT 
CGCGCATATA TCCAAGCCAT GCGGGGAAAG TGTTTACTCC AGTTGAGTGG TCCAGCCGAT 
CCAACGGGAT GTGGAGAGGG ATTTTCATAT GTTCGAGTGC CAAACAAGCC CACGCAAACC 
AAGGAGGAGC AAGAGTCGCA GCCTAAACGT TCGGTCACAG GAACAGATGC AGATTTGCGT 
CGTCTGCCAC TCCAGCGTGC AAAAGAGCTG TTGCGGCAGT TCAAGGTGCC CGAGGAGGAG 
ATCAAAAAGC TTTCCCGCTG GGAGGTCATT GACGTGGTGC GCACCCTGTC CACAGAAAAG 
GCCAAGGCCG GTGAAGAGGG AATGGATAAG TTTTCTCGTG GCAACCGGTT CTCCATTGCA 
GAGCATCAGG AGCGTTATAA GGAAGAGTGC CAGCGCATAT TCGATCTGCA AAACAGAGTG 
CTGGCCAGCT CTGAGGTGCT GTCCACAGAT GAGGCAGAGT CCTCGGCCTC TGAGGAATCT 
GATCTCGAAG AACTTGGCAA GAATCTTGAG AACATGCTGT CAAACAAGAA AACCTCGACG 
CAATTGTCAA GGGAACGTGA AGAGCTGGAG CGTCAGGAGT TGCTTCGCCA GCTTGACGAA 
GAACACGGCG GACCAAGTGG TAGTGGAGGA GCCAAGGGAG CCAAAGGAAA GGATGATCCG 
GGACAGCAAA TGCTGGCAAC CAACAACCAG GGCAGGATCC TTCGCATTAC GCGTACCTTT 
AGAGGTAACG ATGGCAAGGA ATATACTCGC GTGGAGACTG TGCGGCGGCA ACCAGTTATC 
GACGCCTACA TCAAGATTCG CACCACTAAG GACGAGCAGT TCATCAAGCA GTTCGCAACG 
CTAGATGAGC AGCAGAAGGA GGAGATGAAG CGCGAAAAGA GACGCATTCA GGAGCAGCTA 
CGTCGCATCA AGCGCAACCA GGAGCGCGAA CGCCTGGCGC AGCTGGCCCA GAACCAGAAG 
CTTCAGCCAG GTGGCATGCC CACTTCCTTG GGTGATCCTA AGAGCTCGGG CGGTCATTCG 
CACAAGGAGC GGGATAGCGG CTACAAGGAG GTCAGCCCTT CGCGCAAGAA GTTCAAGCTT 
AAGCCAGACC TAAAGCTGAA GTGCGGCGCC TGTGGACAGG TTGGTCACAT GCGCACAAAC 
AAAGCCTGTC CCTTGTATTC TGGCATGCAA AGCAGTCTGT CCCAGTCGAA CCCATCTCTG 
GCTGACGATT TTGACGAACA GAGCGAAAAG GAGATGACAA TGGATGACGA TGATCTTGTG 
AATGTCGATG GCACCAAAGT AACGCTCAGC AGTAAGATTC TCAAGCGTCA TGGTGGTGAT 
GATGGCAAGC GTCGCAGCGG ATCTAGCTCT GGTTTCACCT TGAAGGTTCC CCGAGATGCG 
ATGGGCAAGA AGAAACGCAG AGTGGGTGGC GATCTTCATT GTGACTATCT GCAGCGACAC 
AATAAAACGG CCAATCGCAG GCGCACGGAC CCCGTTGTGG TACTGTCCTC TATCCTGGAG 
ATTATCCATA ATGAGCTGCG ATCTATGCCA GATGTATCGC CATTCCTGTT CCCGGTAAGC 
GCAAAAAAGG TTCCCGACTA CTACCGCGTG GTGACCAAGC CCATGGATCT GCAAACGATG 
AGGGAGTATA TTCGCCAAAG GCGCTACACG AGTCGCGAGA TGTTCCTCGA GGATCTCAAG 
CAGATTGTGG ACAACTCGCT GATCTACAAT GGACCGCAGA GTGCATACAC CTTGGCTGCC 
CAACGCATGT TCAGCAGTTG TTTTGAATTG CTCGCAGAGC GCGAAGACAA ACTGATGCGC 
CTCGAGAAGG CAATTAACCC GCTGCTGGAC GACGATGACC AAGTGGCACT CTCCTTTATC 
TTTGACAAGC TGCACTCGCA GATTAAGCAA TTACCAGAGA GCTGGCCTTT CCTTAAGCCT 
GTCAACAAGA AACAGGTTAA GGACTACTAC ACGGTTATCA AGCGACCCAT GGACCTCGAA 
ACTATCGGCA AAAACATTGA AGCTCATCGC TATCACAGTC GTGCCGAGTA TCTGGCTGAT 
ATCGAGTTGA TCGCCACCAA CTGTGAGCAG TACAACGGCA GTGACACCCG CTACACCAAG 
TTCTCAAAGA AGATACTTGA GTATGCCCAA ACCCAGTTAA TTGAGTTTTC GGAGCACTGC 
GGCCAGTTGG AAAATAACAT AGCTAAGACG CAGGAGCGTG CTAGGGAAAA TGCACCAGAG 
TTTGATGAAG CCTGGGGCAA TGATGATTAC AACTTTGACC GTGGCAGTAG GGCCAGTTCA 
CCCGGAGATG ACTACATCGA CGTCGAGGGT CATGGGGGGC ATGCCTCCTC ATCGAACTCT 
ATCCATCGCA GCATGGGCGC CGAGGCCGGT TCGTCACATA CGGCGCCGGC GGTGCGAAAA 
CCAGCTCCTC CTGGTCCTGG TGAGGTGAAG CGCGGAAGGG GTAGGCCCCG CAAGCAGCGC 
GACCCCGTGG AGGAGGATCT CCAATGCTCC ACAGATGACG AGGACGACGA CGAGGAGGAG 
GACTTCCAGG AGGTCTCCGA AGACGAGAAC AATGCGGCGA GCATTTTAGA TCAGGGCGAA 
CGTATCAATG CGCCTGCCGA TGCCATGGAT GGCATGTTTG ACCCCAAGAA CATCAAGACA 
GAGATTGACC TAGAGGCTCA CCAGATGGCA GCAGAGGAGC CGATCGGCGA GGATGACAGC 
CAGCAGGTGG CCGAAGCAAT GGTGCAGTTG AGTGGCGTGG GCGGCTACTA TGCTCAACAG 
CAGCAAGATG AATCCATGGA TGTGGACCCC AACTACGATC CCTCAGATTT CCTCGCCATG 
CACAAGCAGC GCCAGAGCCT CGGCGAGCCC AGCAGCTTGC AGGGTGCTTT CACCAACTTC 
CTATCGCACG AGCAGGATGA TAATGGGCCT TACAATCCCG CCGAAGCCAG CACAAGTGCC 
GCTTCCGGTG CAGACTTAGG AATGGACGCT TCAATGGCCA TGCAAATGGC GCCGGAAATG 
CCTGTCAATA CCATGAACAA CGGAATGGGC ATCGATGATG ATCTGGATAT TTCGGAGAGT 
GACGAGGAAG ACGATGGTTC TCGAGTGCGT ATCAAAAAGG AGGTCTTCGA CGACGGGGAT 
TACGCCTTGC AGCACCAGCA GATGGGACAG GCAGCATCGC AGTCGCAGAT ATACATGGTG 
GATTCGTCCA ACGAGCCCAC GACTCTCGAC TACCAGCAAC CACCGCAACT GGACTTCCAA 
CAAGTGCAGG AAATGGAGCA GTTGCAGCAC CAAGTGATGC CACCAATGCA ATCAGAGCAA 
CTGCAGCAGC AACAGACGCC GCAGGGAGAC AATGATTATG CCTGGACTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Taf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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