Zif cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zif
Entrez Gene ID	40795
Full Name	Zinc-finger protein
Synonyms	CC32,CG10267,Dmel\CG10267,ZIF,anon-WO0118547.391
General protein information	Preferred Names Zinc-finger protein Names CG10267 gene product from transcript CG10267-RC CG10267-PA CG10267-PB CG10267-PC Zif-PA Zif-PB Zif-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83E8-83E8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275390.1	NP_001262319.1	Zinc-finger protein, isoform C
NM_141400.4	NP_649657.1	Zinc-finger protein, isoform A
NM_001202259.2	NP_001189188.1	Zinc-finger protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Zif is a transcription factor that directly represses aPKC transcription.
Title: Interplay between the transcription factor Zif and aPKC regulates neuroblast polarity and self-renewal.

The following Zif gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zif cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04085	NM_141400.4 Latest version!	Drosophila melanogaster Zinc-finger protein (Zif), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04085	NM_001202259.2 Latest version!	Drosophila melanogaster Zinc-finger protein (Zif), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04085	NM_001275390.1 Latest version!	Drosophila melanogaster Zinc-finger protein (Zif), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zif ( NM_001275390.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262319.1 Drosophila melanogaster Zinc-finger protein (Zif), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04085
Clone ID Related Accession (Same CDS sequence)	NM_001275390.1 , NM_141400.4 , NM_001202259.2
Accession Version	NM_001275390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Zinc-finger protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGTCG AGCTTACCCC ACAGACGTGC CGGGTCTGCC TGGCGCAGAG TGAGCGACTG 
CAGCGCCTAG ACGAGATCCG CGAGGAAGGC GAGGAATCGC CGAACGAGAT GTTGATTCAA 
CTTTTGGGAG TCTCATACAG CAATCTGAAC GACAGAGAAC ACATACCCGA TGGCATCTGC 
AAGTCCTGCA AGGTCGAACT CAACATGGCC TACCAGTTTC GCGAGAAGGC GCTGCGCAAG 
CAGATGGAGA TTGAGGAGTA CTGCCGGGAA CTGGGCTTGC TGGACGAATC AGATGTGATG 
ATGATCAAGG AGGAGGACGG CTCGCAGCAG CAATGCGACG AGGAGATGTA CATCCTGGAG 
GAGACCACCA CCGGGGAAGA GGAGCACCAG GAAGAAAAGG GGCACGAGGA GTACTTGGAA 
GTGGACACCA GCGATCAGCA GGAGTGCATT GGCGACACCA TCGAGTACTT GGAGGACAAC 
TACACCATCG AGATGAACTC CGATCAGACC GAGATCGTCC TGGAGTCTGA GAAGCAGTAC 
GAAGAGACAC CATCGCAGCA GTTGGCGCTG CAGGAGGCGG CCAAGGCAAG CCTGAAGGCG 
CGGCGCGGCC GGGTTCGTCG CGGATTAAAC TCCCTAACAA CATCGGACGG CACTGAGAAG 
GGTGGCTATA TCTGCGACGT CTGCGGAAAC TTTTACGAAA AGCGCGGCCG GATGATGGAG 
CATCGCAGAC GCCATGACGG CATCTGCCAA TATGCATGCG AACTATGCGA TGCCAAGTTC 
CAGGTGCGCG AGCAACTGAG GAAGCACATG TACTCCCACA CGGGCAGCAA ACCCTATAAG 
TGCAGCTTCT GCAGTCGTCA ATTCTTCTAC GAAAGTGTTC TCAAGTCTCA TGAAAATGTT 
CACCGTGGCA TCAAGCCCTA CGTGTGCAAG GTGTGCGACA AAGCGTTTGC CTACGCTCAC 
TCCCTGACCA AGCACGAGCT CATCCACTCG GACATCAAAC TGTACCGCTG CGATTATTGC 
AACAAGGACT TCCGTCTGCT GCATCACATG CGCCAGCACG AGGAGACCAA ACTGCACCAG 
AACGCCGTAA TGCTGGCCGA GAGCATGAAG GTGGAAATGG TGGCCGAGCA GGGGGGCGGG 
AACGAGATCC GAATTCAAGC TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262319.1
CDS	140..1306
Translation	MRVELTPQTCRVCLAQSERLQRLDEIREEGEESPNEMLIQLLGVSYSNLNDREHIPDGICKSCKVELNMAYQFREKALRKQMEIEEYCRELGLLDESDVMMIKEEDGSQQQCDEEMYILEETTTGEEEHQEEKGHEEYLEVDTSDQQECIGDTIEYLEDNYTIEMNSDQTEIVLESEKQYEETPSQQLALQEAAKASLKARRGRVRRGLNSLTTSDGTEKGGYICDVCGNFYEKRGRMMEHRRRHDGICQYACELCDAKFQVREQLRKHMYSHTGSKPYKCSFCSRQFFYESVLKSHENVHRGIKPYVCKVCDKAFAYAHSLTKHELIHSDIKLYRCDYCNKDFRLLHHMRQHEETKLHQNAVMLAESMKVEMVAEQGGGNEIRIQAH

Target ORF information:

RefSeq Version	NM_001275390.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Zinc-finger protein (Zif), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275390.1

ORF Insert Sequence:

ATGCGCGTCG AGCTTACCCC ACAGACGTGC CGGGTCTGCC TGGCGCAGAG TGAGCGACTG 
CAGCGCCTAG ACGAGATCCG CGAGGAAGGC GAGGAATCGC CGAACGAGAT GTTGATTCAA 
CTTTTGGGAG TCTCATACAG CAATCTGAAC GACAGAGAAC ACATACCCGA TGGCATCTGC 
AAGTCCTGCA AGGTCGAACT CAACATGGCC TACCAGTTTC GCGAGAAGGC GCTGCGCAAG 
CAGATGGAGA TTGAGGAGTA CTGCCGGGAA CTGGGCTTGC TGGACGAATC AGATGTGATG 
ATGATCAAGG AGGAGGACGG CTCGCAGCAG CAATGCGACG AGGAGATGTA CATCCTGGAG 
GAGACCACCA CCGGGGAAGA GGAGCACCAG GAAGAAAAGG GGCACGAGGA GTACTTGGAA 
GTGGACACCA GCGATCAGCA GGAGTGCATT GGCGACACCA TCGAGTACTT GGAGGACAAC 
TACACCATCG AGATGAACTC CGATCAGACC GAGATCGTCC TGGAGTCTGA GAAGCAGTAC 
GAAGAGACAC CATCGCAGCA GTTGGCGCTG CAGGAGGCGG CCAAGGCAAG CCTGAAGGCG 
CGGCGCGGCC GGGTTCGTCG CGGATTAAAC TCCCTAACAA CATCGGACGG CACTGAGAAG 
GGTGGCTATA TCTGCGACGT CTGCGGAAAC TTTTACGAAA AGCGCGGCCG GATGATGGAG 
CATCGCAGAC GCCATGACGG CATCTGCCAA TATGCATGCG AACTATGCGA TGCCAAGTTC 
CAGGTGCGCG AGCAACTGAG GAAGCACATG TACTCCCACA CGGGCAGCAA ACCCTATAAG 
TGCAGCTTCT GCAGTCGTCA ATTCTTCTAC GAAAGTGTTC TCAAGTCTCA TGAAAATGTT 
CACCGTGGCA TCAAGCCCTA CGTGTGCAAG GTGTGCGACA AAGCGTTTGC CTACGCTCAC 
TCCCTGACCA AGCACGAGCT CATCCACTCG GACATCAAAC TGTACCGCTG CGATTATTGC 
AACAAGGACT TCCGTCTGCT GCATCACATG CGCCAGCACG AGGAGACCAA ACTGCACCAG 
AACGCCGTAA TGCTGGCCGA GAGCATGAAG GTGGAAATGG TGGCCGAGCA GGGGGGCGGG 
AACGAGATCC GAATTCAAGC TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zif ( NM_141400.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649657.1 Drosophila melanogaster Zinc-finger protein (Zif), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04085
Clone ID Related Accession (Same CDS sequence)	NM_001275390.1 , NM_141400.4 , NM_001202259.2
Accession Version	NM_141400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Zinc-finger protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141400.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGTCG AGCTTACCCC ACAGACGTGC CGGGTCTGCC TGGCGCAGAG TGAGCGACTG 
CAGCGCCTAG ACGAGATCCG CGAGGAAGGC GAGGAATCGC CGAACGAGAT GTTGATTCAA 
CTTTTGGGAG TCTCATACAG CAATCTGAAC GACAGAGAAC ACATACCCGA TGGCATCTGC 
AAGTCCTGCA AGGTCGAACT CAACATGGCC TACCAGTTTC GCGAGAAGGC GCTGCGCAAG 
CAGATGGAGA TTGAGGAGTA CTGCCGGGAA CTGGGCTTGC TGGACGAATC AGATGTGATG 
ATGATCAAGG AGGAGGACGG CTCGCAGCAG CAATGCGACG AGGAGATGTA CATCCTGGAG 
GAGACCACCA CCGGGGAAGA GGAGCACCAG GAAGAAAAGG GGCACGAGGA GTACTTGGAA 
GTGGACACCA GCGATCAGCA GGAGTGCATT GGCGACACCA TCGAGTACTT GGAGGACAAC 
TACACCATCG AGATGAACTC CGATCAGACC GAGATCGTCC TGGAGTCTGA GAAGCAGTAC 
GAAGAGACAC CATCGCAGCA GTTGGCGCTG CAGGAGGCGG CCAAGGCAAG CCTGAAGGCG 
CGGCGCGGCC GGGTTCGTCG CGGATTAAAC TCCCTAACAA CATCGGACGG CACTGAGAAG 
GGTGGCTATA TCTGCGACGT CTGCGGAAAC TTTTACGAAA AGCGCGGCCG GATGATGGAG 
CATCGCAGAC GCCATGACGG CATCTGCCAA TATGCATGCG AACTATGCGA TGCCAAGTTC 
CAGGTGCGCG AGCAACTGAG GAAGCACATG TACTCCCACA CGGGCAGCAA ACCCTATAAG 
TGCAGCTTCT GCAGTCGTCA ATTCTTCTAC GAAAGTGTTC TCAAGTCTCA TGAAAATGTT 
CACCGTGGCA TCAAGCCCTA CGTGTGCAAG GTGTGCGACA AAGCGTTTGC CTACGCTCAC 
TCCCTGACCA AGCACGAGCT CATCCACTCG GACATCAAAC TGTACCGCTG CGATTATTGC 
AACAAGGACT TCCGTCTGCT GCATCACATG CGCCAGCACG AGGAGACCAA ACTGCACCAG 
AACGCCGTAA TGCTGGCCGA GAGCATGAAG GTGGAAATGG TGGCCGAGCA GGGGGGCGGG 
AACGAGATCC GAATTCAAGC TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649657.1
CDS	148..1314
Translation	MRVELTPQTCRVCLAQSERLQRLDEIREEGEESPNEMLIQLLGVSYSNLNDREHIPDGICKSCKVELNMAYQFREKALRKQMEIEEYCRELGLLDESDVMMIKEEDGSQQQCDEEMYILEETTTGEEEHQEEKGHEEYLEVDTSDQQECIGDTIEYLEDNYTIEMNSDQTEIVLESEKQYEETPSQQLALQEAAKASLKARRGRVRRGLNSLTTSDGTEKGGYICDVCGNFYEKRGRMMEHRRRHDGICQYACELCDAKFQVREQLRKHMYSHTGSKPYKCSFCSRQFFYESVLKSHENVHRGIKPYVCKVCDKAFAYAHSLTKHELIHSDIKLYRCDYCNKDFRLLHHMRQHEETKLHQNAVMLAESMKVEMVAEQGGGNEIRIQAH

Target ORF information:

RefSeq Version	NM_141400.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Zinc-finger protein (Zif), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141400.4

ORF Insert Sequence:

ATGCGCGTCG AGCTTACCCC ACAGACGTGC CGGGTCTGCC TGGCGCAGAG TGAGCGACTG 
CAGCGCCTAG ACGAGATCCG CGAGGAAGGC GAGGAATCGC CGAACGAGAT GTTGATTCAA 
CTTTTGGGAG TCTCATACAG CAATCTGAAC GACAGAGAAC ACATACCCGA TGGCATCTGC 
AAGTCCTGCA AGGTCGAACT CAACATGGCC TACCAGTTTC GCGAGAAGGC GCTGCGCAAG 
CAGATGGAGA TTGAGGAGTA CTGCCGGGAA CTGGGCTTGC TGGACGAATC AGATGTGATG 
ATGATCAAGG AGGAGGACGG CTCGCAGCAG CAATGCGACG AGGAGATGTA CATCCTGGAG 
GAGACCACCA CCGGGGAAGA GGAGCACCAG GAAGAAAAGG GGCACGAGGA GTACTTGGAA 
GTGGACACCA GCGATCAGCA GGAGTGCATT GGCGACACCA TCGAGTACTT GGAGGACAAC 
TACACCATCG AGATGAACTC CGATCAGACC GAGATCGTCC TGGAGTCTGA GAAGCAGTAC 
GAAGAGACAC CATCGCAGCA GTTGGCGCTG CAGGAGGCGG CCAAGGCAAG CCTGAAGGCG 
CGGCGCGGCC GGGTTCGTCG CGGATTAAAC TCCCTAACAA CATCGGACGG CACTGAGAAG 
GGTGGCTATA TCTGCGACGT CTGCGGAAAC TTTTACGAAA AGCGCGGCCG GATGATGGAG 
CATCGCAGAC GCCATGACGG CATCTGCCAA TATGCATGCG AACTATGCGA TGCCAAGTTC 
CAGGTGCGCG AGCAACTGAG GAAGCACATG TACTCCCACA CGGGCAGCAA ACCCTATAAG 
TGCAGCTTCT GCAGTCGTCA ATTCTTCTAC GAAAGTGTTC TCAAGTCTCA TGAAAATGTT 
CACCGTGGCA TCAAGCCCTA CGTGTGCAAG GTGTGCGACA AAGCGTTTGC CTACGCTCAC 
TCCCTGACCA AGCACGAGCT CATCCACTCG GACATCAAAC TGTACCGCTG CGATTATTGC 
AACAAGGACT TCCGTCTGCT GCATCACATG CGCCAGCACG AGGAGACCAA ACTGCACCAG 
AACGCCGTAA TGCTGGCCGA GAGCATGAAG GTGGAAATGG TGGCCGAGCA GGGGGGCGGG 
AACGAGATCC GAATTCAAGC TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zif ( NM_001202259.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189188.1 Drosophila melanogaster Zinc-finger protein (Zif), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04085
Clone ID Related Accession (Same CDS sequence)	NM_001275390.1 , NM_141400.4 , NM_001202259.2
Accession Version	NM_001202259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Zinc-finger protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001202259.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGTCG AGCTTACCCC ACAGACGTGC CGGGTCTGCC TGGCGCAGAG TGAGCGACTG 
CAGCGCCTAG ACGAGATCCG CGAGGAAGGC GAGGAATCGC CGAACGAGAT GTTGATTCAA 
CTTTTGGGAG TCTCATACAG CAATCTGAAC GACAGAGAAC ACATACCCGA TGGCATCTGC 
AAGTCCTGCA AGGTCGAACT CAACATGGCC TACCAGTTTC GCGAGAAGGC GCTGCGCAAG 
CAGATGGAGA TTGAGGAGTA CTGCCGGGAA CTGGGCTTGC TGGACGAATC AGATGTGATG 
ATGATCAAGG AGGAGGACGG CTCGCAGCAG CAATGCGACG AGGAGATGTA CATCCTGGAG 
GAGACCACCA CCGGGGAAGA GGAGCACCAG GAAGAAAAGG GGCACGAGGA GTACTTGGAA 
GTGGACACCA GCGATCAGCA GGAGTGCATT GGCGACACCA TCGAGTACTT GGAGGACAAC 
TACACCATCG AGATGAACTC CGATCAGACC GAGATCGTCC TGGAGTCTGA GAAGCAGTAC 
GAAGAGACAC CATCGCAGCA GTTGGCGCTG CAGGAGGCGG CCAAGGCAAG CCTGAAGGCG 
CGGCGCGGCC GGGTTCGTCG CGGATTAAAC TCCCTAACAA CATCGGACGG CACTGAGAAG 
GGTGGCTATA TCTGCGACGT CTGCGGAAAC TTTTACGAAA AGCGCGGCCG GATGATGGAG 
CATCGCAGAC GCCATGACGG CATCTGCCAA TATGCATGCG AACTATGCGA TGCCAAGTTC 
CAGGTGCGCG AGCAACTGAG GAAGCACATG TACTCCCACA CGGGCAGCAA ACCCTATAAG 
TGCAGCTTCT GCAGTCGTCA ATTCTTCTAC GAAAGTGTTC TCAAGTCTCA TGAAAATGTT 
CACCGTGGCA TCAAGCCCTA CGTGTGCAAG GTGTGCGACA AAGCGTTTGC CTACGCTCAC 
TCCCTGACCA AGCACGAGCT CATCCACTCG GACATCAAAC TGTACCGCTG CGATTATTGC 
AACAAGGACT TCCGTCTGCT GCATCACATG CGCCAGCACG AGGAGACCAA ACTGCACCAG 
AACGCCGTAA TGCTGGCCGA GAGCATGAAG GTGGAAATGG TGGCCGAGCA GGGGGGCGGG 
AACGAGATCC GAATTCAAGC TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189188.1
CDS	79..1245
Translation	MRVELTPQTCRVCLAQSERLQRLDEIREEGEESPNEMLIQLLGVSYSNLNDREHIPDGICKSCKVELNMAYQFREKALRKQMEIEEYCRELGLLDESDVMMIKEEDGSQQQCDEEMYILEETTTGEEEHQEEKGHEEYLEVDTSDQQECIGDTIEYLEDNYTIEMNSDQTEIVLESEKQYEETPSQQLALQEAAKASLKARRGRVRRGLNSLTTSDGTEKGGYICDVCGNFYEKRGRMMEHRRRHDGICQYACELCDAKFQVREQLRKHMYSHTGSKPYKCSFCSRQFFYESVLKSHENVHRGIKPYVCKVCDKAFAYAHSLTKHELIHSDIKLYRCDYCNKDFRLLHHMRQHEETKLHQNAVMLAESMKVEMVAEQGGGNEIRIQAH

Target ORF information:

RefSeq Version	NM_001202259.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Zinc-finger protein (Zif), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202259.2

ORF Insert Sequence:

ATGCGCGTCG AGCTTACCCC ACAGACGTGC CGGGTCTGCC TGGCGCAGAG TGAGCGACTG 
CAGCGCCTAG ACGAGATCCG CGAGGAAGGC GAGGAATCGC CGAACGAGAT GTTGATTCAA 
CTTTTGGGAG TCTCATACAG CAATCTGAAC GACAGAGAAC ACATACCCGA TGGCATCTGC 
AAGTCCTGCA AGGTCGAACT CAACATGGCC TACCAGTTTC GCGAGAAGGC GCTGCGCAAG 
CAGATGGAGA TTGAGGAGTA CTGCCGGGAA CTGGGCTTGC TGGACGAATC AGATGTGATG 
ATGATCAAGG AGGAGGACGG CTCGCAGCAG CAATGCGACG AGGAGATGTA CATCCTGGAG 
GAGACCACCA CCGGGGAAGA GGAGCACCAG GAAGAAAAGG GGCACGAGGA GTACTTGGAA 
GTGGACACCA GCGATCAGCA GGAGTGCATT GGCGACACCA TCGAGTACTT GGAGGACAAC 
TACACCATCG AGATGAACTC CGATCAGACC GAGATCGTCC TGGAGTCTGA GAAGCAGTAC 
GAAGAGACAC CATCGCAGCA GTTGGCGCTG CAGGAGGCGG CCAAGGCAAG CCTGAAGGCG 
CGGCGCGGCC GGGTTCGTCG CGGATTAAAC TCCCTAACAA CATCGGACGG CACTGAGAAG 
GGTGGCTATA TCTGCGACGT CTGCGGAAAC TTTTACGAAA AGCGCGGCCG GATGATGGAG 
CATCGCAGAC GCCATGACGG CATCTGCCAA TATGCATGCG AACTATGCGA TGCCAAGTTC 
CAGGTGCGCG AGCAACTGAG GAAGCACATG TACTCCCACA CGGGCAGCAA ACCCTATAAG 
TGCAGCTTCT GCAGTCGTCA ATTCTTCTAC GAAAGTGTTC TCAAGTCTCA TGAAAATGTT 
CACCGTGGCA TCAAGCCCTA CGTGTGCAAG GTGTGCGACA AAGCGTTTGC CTACGCTCAC 
TCCCTGACCA AGCACGAGCT CATCCACTCG GACATCAAAC TGTACCGCTG CGATTATTGC 
AACAAGGACT TCCGTCTGCT GCATCACATG CGCCAGCACG AGGAGACCAA ACTGCACCAG 
AACGCCGTAA TGCTGGCCGA GAGCATGAAG GTGGAAATGG TGGCCGAGCA GGGGGGCGGG 
AACGAGATCC GAATTCAAGC TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Zif cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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