TfIIFalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	TfIIFalpha
Entrez Gene ID	40790
Full Name	Transcription factor IIFalpha
Synonyms	154355_at,CG10281,DmTFIIF,Dmel\CG10281,F5a,TFIIF,TFIIF-L,TFIIFa,TFIIFalpha,TfIIF
General protein information	Preferred Names Transcription factor IIFalpha Names CG10281 gene product from transcript CG10281-RA CG10281-PA TFIIF large subunit TfIIFalpha-PA factor 5 factor 5a large subunit of transcription factor 5 transcription factor IIFalpha
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83E5-83E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_079522.4	NP_524246.1	transcription factor IIFalpha

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TfIIFalpha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TfIIFalpha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04079	NM_079522.4 Latest version!	Drosophila melanogaster transcription factor IIFalpha (TfIIFalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TfIIFalpha ( NM_079522.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524246.1 Drosophila melanogaster transcription factor IIFalpha (TfIIFalpha), mRNA.

Price & Availability↑

CloneID	OFa04079
Clone ID Related Accession (Same CDS sequence)	NM_079522.4
Accession Version	NM_079522.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transcription factor IIFalpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079522.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCG CGTCAAAGTC GACGCCGAGC GCTGCCAGCG GGAGCTCCAC GTCCGCAGCT 
GCCGCCGCCG CAGCCTCTGT CGCCTCAGGA TCCGCCTCGT CATCCGCCAA CGTGCAGGAG 
TTCAAGATCC GCGTGCCCAA GATGCCCAAG AAGCACCATG TGATGCGGTT CAACGCCACG 
CTCAATGTGG ATTTCGCGCA GTGGAGAAAT GTGAAACTGG AGCGGGAAAA CAACATGAAG 
GAGTTCCGGG GCATGGAGGA GGATCAGCCC AAGTTTGGTG CTGGATCGGA GTACAACCGG 
GATCAGCGCG AGGAGGCGCG CCGCAAGAAG TTTGGCATTA TAGCGAGAAA GTACCGTCCA 
GAGGCACAGC CATGGATCCT TAAAGTGGGT GGCAAGACTG GAAAGAAGTT CAAAGGCATC 
CGCGAGGGCG GCGTAGGCGA GAATGCAGCT TTCTATGTGT TCACCCATGC ACCAGACGGC 
GCCATTGAGG CATACCCCTT GACCGAGTGG TACAACTTCC AGCCTATTCA GCGCTACAAA 
TCGCTGTCGG CGGAGGAGGC CGAACAGGAA TTTGGTCGGC GCAAAAAGGT GATGAACTAC 
TTCTCCCTTA TGTTGCGAAA GCGTCTGCGC GGCGATGAGG AAGAGGAGCA GGATCCGGAG 
GAGGCTAAGC TAATTAAGGC AGCCACCAAA AAATCCAAGG AGCTCAAGAT CACTGACATG 
GATGAATGGA TTGATTCCGA GGATGAGTCG GATTCGGAGG ACGAGGAGGA TAAAAAGAAG 
AAGGAGCAGG AAGACAGCGA CGATGGCAAG GCCAAGGGAA AGGGCAAGAA GGGCGCAGAC 
AAGAAAAAGA AAAAGAGGGA TGTTGATGAC GAGGCCTTCG AGGAGTCGGA CGATGGTGAC 
GAGGAGGGCA GAGAAATGGA CTACGACACC AGCTCCAGTG AAGACGAACC AGATCCAGAG 
GCAAAGGTGG ACAAGGACAT GAAAGGCGTG GCCGAGGAAG ATGCGCTCCG CAAGCTGCTT 
ACCTCCGATG AAGAGGAGGA TGATGAGAAG AAGTCTGATG AGTCGGACAA GGAGGATGCC 
GACGGCGAGA AAAAAAAGAA GGATAAGGGC AAGGACGAGG TATCCAAGGA CAAAAAGAAG 
AAGAAACCCA CAAAGGACGA CAAGAAGGGC AAGTCGAATG GCAGCGGTGA CTCGTCCACA 
GACTTCAGCT CTGACAGCAC AGACTCCGAG GACGACCTTA GCAACGGACC GCCCAAGAAG 
AAGGTCGTCG TCAAGGACAA AGACAAGGAA AAGGAAAAGG AGAAAGAGAG CGCTGCGAGC 
AGCAAGGTCA TCGCCAGCAG CAGCAATGCC AATAAATCTC GCTCGGCCAC TCCAACGCTC 
TCGACGGATG CCAGCAAGCG CAAGATGAAC AGCTTACCCA GCGATCTCAC CGCTTCGGAC 
ACAAGCAACA GTCCCACCAG CACGCCAGCA AAGAGGCCTA AGAACGAGAT TAGCACATCG 
CTGCCCACTT CCTTCTCTGG CGGCAAAGTA GAGGACTACG GCATCACCGA GGAGGCGGTG 
CGTCGGTATC TTAAGCGAAA GCCCCTGACC GCCACCGAGT TGCTCACCAA GTTCAAGAAC 
AAGAAGACGC CTGTCTCCAG CGACCGTTTG GTCGAGACCA TGACGAAAAT CCTGAAGAAG 
ATCAATCCCG TCAAGCACAC CATCCAGGGG AAAATGTATC TCTGGATCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524246.1
CDS	130..1863
Translation	MSSASKSTPSAASGSSTSAAAAAAASVASGSASSSANVQEFKIRVPKMPKKHHVMRFNATLNVDFAQWRNVKLERENNMKEFRGMEEDQPKFGAGSEYNRDQREEARRKKFGIIARKYRPEAQPWILKVGGKTGKKFKGIREGGVGENAAFYVFTHAPDGAIEAYPLTEWYNFQPIQRYKSLSAEEAEQEFGRRKKVMNYFSLMLRKRLRGDEEEEQDPEEAKLIKAATKKSKELKITDMDEWIDSEDESDSEDEEDKKKKEQEDSDDGKAKGKGKKGADKKKKKRDVDDEAFEESDDGDEEGREMDYDTSSSEDEPDPEAKVDKDMKGVAEEDALRKLLTSDEEEDDEKKSDESDKEDADGEKKKKDKGKDEVSKDKKKKKPTKDDKKGKSNGSGDSSTDFSSDSTDSEDDLSNGPPKKKVVVKDKDKEKEKEKESAASSKVIASSSNANKSRSATPTLSTDASKRKMNSLPSDLTASDTSNSPTSTPAKRPKNEISTSLPTSFSGGKVEDYGITEEAVRRYLKRKPLTATELLTKFKNKKTPVSSDRLVETMTKILKKINPVKHTIQGKMYLWIK

Target ORF information:

RefSeq Version	NM_079522.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transcription factor IIFalpha (TfIIFalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079522.4

ORF Insert Sequence:

ATGTCGAGCG CGTCAAAGTC GACGCCGAGC GCTGCCAGCG GGAGCTCCAC GTCCGCAGCT 
GCCGCCGCCG CAGCCTCTGT CGCCTCAGGA TCCGCCTCGT CATCCGCCAA CGTGCAGGAG 
TTCAAGATCC GCGTGCCCAA GATGCCCAAG AAGCACCATG TGATGCGGTT CAACGCCACG 
CTCAATGTGG ATTTCGCGCA GTGGAGAAAT GTGAAACTGG AGCGGGAAAA CAACATGAAG 
GAGTTCCGGG GCATGGAGGA GGATCAGCCC AAGTTTGGTG CTGGATCGGA GTACAACCGG 
GATCAGCGCG AGGAGGCGCG CCGCAAGAAG TTTGGCATTA TAGCGAGAAA GTACCGTCCA 
GAGGCACAGC CATGGATCCT TAAAGTGGGT GGCAAGACTG GAAAGAAGTT CAAAGGCATC 
CGCGAGGGCG GCGTAGGCGA GAATGCAGCT TTCTATGTGT TCACCCATGC ACCAGACGGC 
GCCATTGAGG CATACCCCTT GACCGAGTGG TACAACTTCC AGCCTATTCA GCGCTACAAA 
TCGCTGTCGG CGGAGGAGGC CGAACAGGAA TTTGGTCGGC GCAAAAAGGT GATGAACTAC 
TTCTCCCTTA TGTTGCGAAA GCGTCTGCGC GGCGATGAGG AAGAGGAGCA GGATCCGGAG 
GAGGCTAAGC TAATTAAGGC AGCCACCAAA AAATCCAAGG AGCTCAAGAT CACTGACATG 
GATGAATGGA TTGATTCCGA GGATGAGTCG GATTCGGAGG ACGAGGAGGA TAAAAAGAAG 
AAGGAGCAGG AAGACAGCGA CGATGGCAAG GCCAAGGGAA AGGGCAAGAA GGGCGCAGAC 
AAGAAAAAGA AAAAGAGGGA TGTTGATGAC GAGGCCTTCG AGGAGTCGGA CGATGGTGAC 
GAGGAGGGCA GAGAAATGGA CTACGACACC AGCTCCAGTG AAGACGAACC AGATCCAGAG 
GCAAAGGTGG ACAAGGACAT GAAAGGCGTG GCCGAGGAAG ATGCGCTCCG CAAGCTGCTT 
ACCTCCGATG AAGAGGAGGA TGATGAGAAG AAGTCTGATG AGTCGGACAA GGAGGATGCC 
GACGGCGAGA AAAAAAAGAA GGATAAGGGC AAGGACGAGG TATCCAAGGA CAAAAAGAAG 
AAGAAACCCA CAAAGGACGA CAAGAAGGGC AAGTCGAATG GCAGCGGTGA CTCGTCCACA 
GACTTCAGCT CTGACAGCAC AGACTCCGAG GACGACCTTA GCAACGGACC GCCCAAGAAG 
AAGGTCGTCG TCAAGGACAA AGACAAGGAA AAGGAAAAGG AGAAAGAGAG CGCTGCGAGC 
AGCAAGGTCA TCGCCAGCAG CAGCAATGCC AATAAATCTC GCTCGGCCAC TCCAACGCTC 
TCGACGGATG CCAGCAAGCG CAAGATGAAC AGCTTACCCA GCGATCTCAC CGCTTCGGAC 
ACAAGCAACA GTCCCACCAG CACGCCAGCA AAGAGGCCTA AGAACGAGAT TAGCACATCG 
CTGCCCACTT CCTTCTCTGG CGGCAAAGTA GAGGACTACG GCATCACCGA GGAGGCGGTG 
CGTCGGTATC TTAAGCGAAA GCCCCTGACC GCCACCGAGT TGCTCACCAA GTTCAAGAAC 
AAGAAGACGC CTGTCTCCAG CGACCGTTTG GTCGAGACCA TGACGAAAAT CCTGAAGAAG 
ATCAATCCCG TCAAGCACAC CATCCAGGGG AAAATGTATC TCTGGATCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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TfIIFalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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