Madm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Madm
Entrez Gene ID	40710
Full Name	MLF1-adaptor molecule
Synonyms	BcDNA:LD28657,CG1098,Dmel\CG1098,MADM,anon-WO0118547.406,dMADM,lincRNA.S6031
General protein information	Preferred Names MLF1-adaptor molecule Names CG1098 gene product from transcript CG1098-RB CG1098-PA CG1098-PB Madm-PA Madm-PB Mif1 adaptor molecule
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83C2-83C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001316491.1	NP_001303420.1	MLF1-adaptor molecule, isoform B
NM_141324.3	NP_649581.1	MLF1-adaptor molecule, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nrbp1	NP_671734.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007996	XP_317472.4
Bos taurus (cattle)	NRBP1	NP_001070357.1
Danio rerio (zebrafish)	nrbp1	NP_001038654.2
Xenopus tropicalis (tropical clawed frog)	nrbp1	NP_001005659.1
Pan troglodytes (chimpanzee)	NRBP1	XP_515359.3
Drosophila melanogaster (fruit fly)	Madm	NP_649581.1
Homo sapiens (human)	NRBP1	NP_037524.1
Canis lupus familiaris (dog)	NRBP1	XP_532911.4
Rattus norvegicus (Norway rat)	Nrbp1	NP_001030169.1
	NRBP1	XP_001097198.2
Gallus gallus (chicken)	NRBP1	NP_001006420.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Madm is a novel tumour suppressor that regulates the competition between germline stem cells and somatic cyst stem cells for niche occupancy.
Title: The novel tumour suppressor Madm regulates stem cell competition in the Drosophila testis.

The data suggest the model that Bunched and Madm functionally interact with the TOR pathway in the cytoplasm to regulate the growth and subsequent division of intestinal stem cells.
Title: Bunched and Madm Function Downstream of Tuberous Sclerosis Complex to Regulate the Growth of Intestinal Stem Cells in Drosophila.

The following Madm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Madm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03992	NM_001316491.1 Latest version!	Drosophila melanogaster MLF1-adaptor molecule (Madm), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa03992	NM_141324.3 Latest version!	Drosophila melanogaster MLF1-adaptor molecule (Madm), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Madm ( NM_001316491.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303420.1 Drosophila melanogaster MLF1-adaptor molecule (Madm), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03992
Clone ID Related Accession (Same CDS sequence)	NM_001316491.1 , NM_141324.3
Accession Version	NM_001316491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	MLF1-adaptor molecule, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAAATA GCCAAGCGAA TGCCGGCATC AGCGGTAGCA CGGTAGCGGA TGAACCCATC 
CAGCACCACC CTTCCTTGGC GGCCGGCCCC GTAAGTGCAT CCTGCCCAGC AGCGACCCCG 
CCGTCGCAGT CGACGCAGCA GCCCCCGCCG CACATAGTTA GTGCCTCAAC AGCTGATGCG 
GGCAGCAGCG CTGCGGTCGG CGTCGGCGTC GTAGCTGGCA GCGAGGGCGT CAATCTGGAC 
TCGTCGCCAC GCGAGTCCGG GGACGATTCG GAGGATGAGA GCGAGATTTT GGAGGAGTCG 
CCATGTGGAC GCTGGCTGAA GCGACGCGAG GAGGTGGATC AGCGTGACGT ACCCGGTATT 
GACTGCGTCC ATCTGGCCAT GGACACCGAG GAGGGCGTAG AGGTCGTGTG GAATGAGGTG 
CAATATGCCA GCCTACAGGA GCTGAAGTCG CAGGAGGAGA AGATGCGGCA GGTGTTCGAC 
AATCTGCTGC AGCTGGACCA CCAAAACATT GTCAAGTTCC ACCGCTACTG GACGGACACG 
CAGCAGGCCG AGCGACCCAG GGTGGTCTTT ATCACCGAGT ACATGTCGAG CGGATCGCTG 
AAACAGTTCC TCAAGCGCAC CAAGCGCAAC GCCAAGCGGC TACCGCTGGA GTCGTGGCGC 
CGGTGGTGCA CTCAGATTTT GTCCGCACTC AGCTATTTGC ATTCCTGCTC GCCGCCCATC 
ATCCACGGCA ATTTAACCTG TGATAGCATC TTCATCCAGC ACAACGGCCT GGTCAAGATC 
GGCTCGGTGG TCCCGGACGC GGTCCACTAC AGCGTCCGGC GCGGCCGAGA GCGGGAGCGT 
GAACGCGAAC GAGGCGCCCA CTACTTCCAG GCGCCCGAGT ACGGCGCCGC CGACCAGTTG 
ACCGCCGCCC TCGACATCTA CGCCTTCGGG ATGTGTGCCC TGGAGATGGC CGCCCTGGAA 
ATCCAGCCCA GTAACAGCGA GTCAACTGCC ATCAACGAGG AGACCATCCA GCGCACAATC 
TTTAGCCTGG AAAACGATCT GCAGCGCGAC CTGATACGAA AGTGTCTCAA CCCGCAGCCG 
CAGGACCGAC CCAGCGCCAA TGATCTGCTC TTTCACCCAT TGCTTTTCGA GGTGCACTCC 
CTCAAACTGC TGACTGCCCA TTGCCTGGTC TTCTCGCCCG CCAACCGCAC TATGTTCTCG 
GAAACCGCCT TCGACGGCCT CATGCAGCGA TACTACCAGC CGGACGTGGT TATGGCCCAG 
CTGCGGCTGG CCGGCGGCCA GGAGCGGCAG TACCGACTCG CAGACGTTTC AGGCGCCGAT 
AAGCTGGAAA AGTTCGTCGA GGACGTGAAG TATGGTGTGT ATCCGCTAAT TACGTACAGC 
GGCAAGAAGC CGCCCAACTT CCGTTCCCGG GCCGCCTCCC CGGAACGGGC TGACTCCGTA 
AAATCGGCCA CGCCGGAGCC TGTGGACACG GAGTCGCGGC GCATCGTTAA CATGATGTGC 
AGCGTTAAAA TCAAAGAGGA TAGCAATGAC ATAACCATGA CAATACTGCT GCGCATGGAC 
GACAAGATGA ACCGCCAACT GACATGCCAG GTGAACGAGA ACGATACGGC GGCGGATCTC 
ACCAGCGAAC TGGTGCGCCT CGGTTTCGTC CACCTCGACG ACCAGGACAA GATCCAGGTG 
CTGCTGGAGG AAACGCTTAA AGCGGGCGTT ATGAGCGACG GTGCCGGGGC AGAGAGCTCC 
GGTGCGGGGG TGACGACCAC GGCGACCATG GCGGCCTTGG AGCAGCTGGA GCGCAACTGG 
TCGATATCCT CGGATGCAGA CAAGCAGGGC ACTGCCGTGA TGTATGTACC GCAGGAGCAG 
CAGAACGCGG ACGGGGACGT CGACGTAGAA CACTCGGGCA CGACGAGCAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303420.1
CDS	476..2389
Translation	MSNSQANAGISGSTVADEPIQHHPSLAAGPVSASCPAATPPSQSTQQPPPHIVSASTADAGSSAAVGVGVVAGSEGVNLDSSPRESGDDSEDESEILEESPCGRWLKRREEVDQRDVPGIDCVHLAMDTEEGVEVVWNEVQYASLQELKSQEEKMRQVFDNLLQLDHQNIVKFHRYWTDTQQAERPRVVFITEYMSSGSLKQFLKRTKRNAKRLPLESWRRWCTQILSALSYLHSCSPPIIHGNLTCDSIFIQHNGLVKIGSVVPDAVHYSVRRGRERERERERGAHYFQAPEYGAADQLTAALDIYAFGMCALEMAALEIQPSNSESTAINEETIQRTIFSLENDLQRDLIRKCLNPQPQDRPSANDLLFHPLLFEVHSLKLLTAHCLVFSPANRTMFSETAFDGLMQRYYQPDVVMAQLRLAGGQERQYRLADVSGADKLEKFVEDVKYGVYPLITYSGKKPPNFRSRAASPERADSVKSATPEPVDTESRRIVNMMCSVKIKEDSNDITMTILLRMDDKMNRQLTCQVNENDTAADLTSELVRLGFVHLDDQDKIQVLLEETLKAGVMSDGAGAESSGAGVTTTATMAALEQLERNWSISSDADKQGTAVMYVPQEQQNADGDVDVEHSGTTSN

Target ORF information:

RefSeq Version	NM_001316491.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MLF1-adaptor molecule (Madm), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316491.1

ORF Insert Sequence:

ATGTCAAATA GCCAAGCGAA TGCCGGCATC AGCGGTAGCA CGGTAGCGGA TGAACCCATC 
CAGCACCACC CTTCCTTGGC GGCCGGCCCC GTAAGTGCAT CCTGCCCAGC AGCGACCCCG 
CCGTCGCAGT CGACGCAGCA GCCCCCGCCG CACATAGTTA GTGCCTCAAC AGCTGATGCG 
GGCAGCAGCG CTGCGGTCGG CGTCGGCGTC GTAGCTGGCA GCGAGGGCGT CAATCTGGAC 
TCGTCGCCAC GCGAGTCCGG GGACGATTCG GAGGATGAGA GCGAGATTTT GGAGGAGTCG 
CCATGTGGAC GCTGGCTGAA GCGACGCGAG GAGGTGGATC AGCGTGACGT ACCCGGTATT 
GACTGCGTCC ATCTGGCCAT GGACACCGAG GAGGGCGTAG AGGTCGTGTG GAATGAGGTG 
CAATATGCCA GCCTACAGGA GCTGAAGTCG CAGGAGGAGA AGATGCGGCA GGTGTTCGAC 
AATCTGCTGC AGCTGGACCA CCAAAACATT GTCAAGTTCC ACCGCTACTG GACGGACACG 
CAGCAGGCCG AGCGACCCAG GGTGGTCTTT ATCACCGAGT ACATGTCGAG CGGATCGCTG 
AAACAGTTCC TCAAGCGCAC CAAGCGCAAC GCCAAGCGGC TACCGCTGGA GTCGTGGCGC 
CGGTGGTGCA CTCAGATTTT GTCCGCACTC AGCTATTTGC ATTCCTGCTC GCCGCCCATC 
ATCCACGGCA ATTTAACCTG TGATAGCATC TTCATCCAGC ACAACGGCCT GGTCAAGATC 
GGCTCGGTGG TCCCGGACGC GGTCCACTAC AGCGTCCGGC GCGGCCGAGA GCGGGAGCGT 
GAACGCGAAC GAGGCGCCCA CTACTTCCAG GCGCCCGAGT ACGGCGCCGC CGACCAGTTG 
ACCGCCGCCC TCGACATCTA CGCCTTCGGG ATGTGTGCCC TGGAGATGGC CGCCCTGGAA 
ATCCAGCCCA GTAACAGCGA GTCAACTGCC ATCAACGAGG AGACCATCCA GCGCACAATC 
TTTAGCCTGG AAAACGATCT GCAGCGCGAC CTGATACGAA AGTGTCTCAA CCCGCAGCCG 
CAGGACCGAC CCAGCGCCAA TGATCTGCTC TTTCACCCAT TGCTTTTCGA GGTGCACTCC 
CTCAAACTGC TGACTGCCCA TTGCCTGGTC TTCTCGCCCG CCAACCGCAC TATGTTCTCG 
GAAACCGCCT TCGACGGCCT CATGCAGCGA TACTACCAGC CGGACGTGGT TATGGCCCAG 
CTGCGGCTGG CCGGCGGCCA GGAGCGGCAG TACCGACTCG CAGACGTTTC AGGCGCCGAT 
AAGCTGGAAA AGTTCGTCGA GGACGTGAAG TATGGTGTGT ATCCGCTAAT TACGTACAGC 
GGCAAGAAGC CGCCCAACTT CCGTTCCCGG GCCGCCTCCC CGGAACGGGC TGACTCCGTA 
AAATCGGCCA CGCCGGAGCC TGTGGACACG GAGTCGCGGC GCATCGTTAA CATGATGTGC 
AGCGTTAAAA TCAAAGAGGA TAGCAATGAC ATAACCATGA CAATACTGCT GCGCATGGAC 
GACAAGATGA ACCGCCAACT GACATGCCAG GTGAACGAGA ACGATACGGC GGCGGATCTC 
ACCAGCGAAC TGGTGCGCCT CGGTTTCGTC CACCTCGACG ACCAGGACAA GATCCAGGTG 
CTGCTGGAGG AAACGCTTAA AGCGGGCGTT ATGAGCGACG GTGCCGGGGC AGAGAGCTCC 
GGTGCGGGGG TGACGACCAC GGCGACCATG GCGGCCTTGG AGCAGCTGGA GCGCAACTGG 
TCGATATCCT CGGATGCAGA CAAGCAGGGC ACTGCCGTGA TGTATGTACC GCAGGAGCAG 
CAGAACGCGG ACGGGGACGT CGACGTAGAA CACTCGGGCA CGACGAGCAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Madm ( NM_141324.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649581.1 Drosophila melanogaster MLF1-adaptor molecule (Madm), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03992
Clone ID Related Accession (Same CDS sequence)	NM_001316491.1 , NM_141324.3
Accession Version	NM_141324.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	MLF1-adaptor molecule, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141324.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAAATA GCCAAGCGAA TGCCGGCATC AGCGGTAGCA CGGTAGCGGA TGAACCCATC 
CAGCACCACC CTTCCTTGGC GGCCGGCCCC GTAAGTGCAT CCTGCCCAGC AGCGACCCCG 
CCGTCGCAGT CGACGCAGCA GCCCCCGCCG CACATAGTTA GTGCCTCAAC AGCTGATGCG 
GGCAGCAGCG CTGCGGTCGG CGTCGGCGTC GTAGCTGGCA GCGAGGGCGT CAATCTGGAC 
TCGTCGCCAC GCGAGTCCGG GGACGATTCG GAGGATGAGA GCGAGATTTT GGAGGAGTCG 
CCATGTGGAC GCTGGCTGAA GCGACGCGAG GAGGTGGATC AGCGTGACGT ACCCGGTATT 
GACTGCGTCC ATCTGGCCAT GGACACCGAG GAGGGCGTAG AGGTCGTGTG GAATGAGGTG 
CAATATGCCA GCCTACAGGA GCTGAAGTCG CAGGAGGAGA AGATGCGGCA GGTGTTCGAC 
AATCTGCTGC AGCTGGACCA CCAAAACATT GTCAAGTTCC ACCGCTACTG GACGGACACG 
CAGCAGGCCG AGCGACCCAG GGTGGTCTTT ATCACCGAGT ACATGTCGAG CGGATCGCTG 
AAACAGTTCC TCAAGCGCAC CAAGCGCAAC GCCAAGCGGC TACCGCTGGA GTCGTGGCGC 
CGGTGGTGCA CTCAGATTTT GTCCGCACTC AGCTATTTGC ATTCCTGCTC GCCGCCCATC 
ATCCACGGCA ATTTAACCTG TGATAGCATC TTCATCCAGC ACAACGGCCT GGTCAAGATC 
GGCTCGGTGG TCCCGGACGC GGTCCACTAC AGCGTCCGGC GCGGCCGAGA GCGGGAGCGT 
GAACGCGAAC GAGGCGCCCA CTACTTCCAG GCGCCCGAGT ACGGCGCCGC CGACCAGTTG 
ACCGCCGCCC TCGACATCTA CGCCTTCGGG ATGTGTGCCC TGGAGATGGC CGCCCTGGAA 
ATCCAGCCCA GTAACAGCGA GTCAACTGCC ATCAACGAGG AGACCATCCA GCGCACAATC 
TTTAGCCTGG AAAACGATCT GCAGCGCGAC CTGATACGAA AGTGTCTCAA CCCGCAGCCG 
CAGGACCGAC CCAGCGCCAA TGATCTGCTC TTTCACCCAT TGCTTTTCGA GGTGCACTCC 
CTCAAACTGC TGACTGCCCA TTGCCTGGTC TTCTCGCCCG CCAACCGCAC TATGTTCTCG 
GAAACCGCCT TCGACGGCCT CATGCAGCGA TACTACCAGC CGGACGTGGT TATGGCCCAG 
CTGCGGCTGG CCGGCGGCCA GGAGCGGCAG TACCGACTCG CAGACGTTTC AGGCGCCGAT 
AAGCTGGAAA AGTTCGTCGA GGACGTGAAG TATGGTGTGT ATCCGCTAAT TACGTACAGC 
GGCAAGAAGC CGCCCAACTT CCGTTCCCGG GCCGCCTCCC CGGAACGGGC TGACTCCGTA 
AAATCGGCCA CGCCGGAGCC TGTGGACACG GAGTCGCGGC GCATCGTTAA CATGATGTGC 
AGCGTTAAAA TCAAAGAGGA TAGCAATGAC ATAACCATGA CAATACTGCT GCGCATGGAC 
GACAAGATGA ACCGCCAACT GACATGCCAG GTGAACGAGA ACGATACGGC GGCGGATCTC 
ACCAGCGAAC TGGTGCGCCT CGGTTTCGTC CACCTCGACG ACCAGGACAA GATCCAGGTG 
CTGCTGGAGG AAACGCTTAA AGCGGGCGTT ATGAGCGACG GTGCCGGGGC AGAGAGCTCC 
GGTGCGGGGG TGACGACCAC GGCGACCATG GCGGCCTTGG AGCAGCTGGA GCGCAACTGG 
TCGATATCCT CGGATGCAGA CAAGCAGGGC ACTGCCGTGA TGTATGTACC GCAGGAGCAG 
CAGAACGCGG ACGGGGACGT CGACGTAGAA CACTCGGGCA CGACGAGCAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649581.1
CDS	476..2389
Translation	MSNSQANAGISGSTVADEPIQHHPSLAAGPVSASCPAATPPSQSTQQPPPHIVSASTADAGSSAAVGVGVVAGSEGVNLDSSPRESGDDSEDESEILEESPCGRWLKRREEVDQRDVPGIDCVHLAMDTEEGVEVVWNEVQYASLQELKSQEEKMRQVFDNLLQLDHQNIVKFHRYWTDTQQAERPRVVFITEYMSSGSLKQFLKRTKRNAKRLPLESWRRWCTQILSALSYLHSCSPPIIHGNLTCDSIFIQHNGLVKIGSVVPDAVHYSVRRGRERERERERGAHYFQAPEYGAADQLTAALDIYAFGMCALEMAALEIQPSNSESTAINEETIQRTIFSLENDLQRDLIRKCLNPQPQDRPSANDLLFHPLLFEVHSLKLLTAHCLVFSPANRTMFSETAFDGLMQRYYQPDVVMAQLRLAGGQERQYRLADVSGADKLEKFVEDVKYGVYPLITYSGKKPPNFRSRAASPERADSVKSATPEPVDTESRRIVNMMCSVKIKEDSNDITMTILLRMDDKMNRQLTCQVNENDTAADLTSELVRLGFVHLDDQDKIQVLLEETLKAGVMSDGAGAESSGAGVTTTATMAALEQLERNWSISSDADKQGTAVMYVPQEQQNADGDVDVEHSGTTSN

Target ORF information:

RefSeq Version	NM_141324.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MLF1-adaptor molecule (Madm), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141324.3

ORF Insert Sequence:

ATGTCAAATA GCCAAGCGAA TGCCGGCATC AGCGGTAGCA CGGTAGCGGA TGAACCCATC 
CAGCACCACC CTTCCTTGGC GGCCGGCCCC GTAAGTGCAT CCTGCCCAGC AGCGACCCCG 
CCGTCGCAGT CGACGCAGCA GCCCCCGCCG CACATAGTTA GTGCCTCAAC AGCTGATGCG 
GGCAGCAGCG CTGCGGTCGG CGTCGGCGTC GTAGCTGGCA GCGAGGGCGT CAATCTGGAC 
TCGTCGCCAC GCGAGTCCGG GGACGATTCG GAGGATGAGA GCGAGATTTT GGAGGAGTCG 
CCATGTGGAC GCTGGCTGAA GCGACGCGAG GAGGTGGATC AGCGTGACGT ACCCGGTATT 
GACTGCGTCC ATCTGGCCAT GGACACCGAG GAGGGCGTAG AGGTCGTGTG GAATGAGGTG 
CAATATGCCA GCCTACAGGA GCTGAAGTCG CAGGAGGAGA AGATGCGGCA GGTGTTCGAC 
AATCTGCTGC AGCTGGACCA CCAAAACATT GTCAAGTTCC ACCGCTACTG GACGGACACG 
CAGCAGGCCG AGCGACCCAG GGTGGTCTTT ATCACCGAGT ACATGTCGAG CGGATCGCTG 
AAACAGTTCC TCAAGCGCAC CAAGCGCAAC GCCAAGCGGC TACCGCTGGA GTCGTGGCGC 
CGGTGGTGCA CTCAGATTTT GTCCGCACTC AGCTATTTGC ATTCCTGCTC GCCGCCCATC 
ATCCACGGCA ATTTAACCTG TGATAGCATC TTCATCCAGC ACAACGGCCT GGTCAAGATC 
GGCTCGGTGG TCCCGGACGC GGTCCACTAC AGCGTCCGGC GCGGCCGAGA GCGGGAGCGT 
GAACGCGAAC GAGGCGCCCA CTACTTCCAG GCGCCCGAGT ACGGCGCCGC CGACCAGTTG 
ACCGCCGCCC TCGACATCTA CGCCTTCGGG ATGTGTGCCC TGGAGATGGC CGCCCTGGAA 
ATCCAGCCCA GTAACAGCGA GTCAACTGCC ATCAACGAGG AGACCATCCA GCGCACAATC 
TTTAGCCTGG AAAACGATCT GCAGCGCGAC CTGATACGAA AGTGTCTCAA CCCGCAGCCG 
CAGGACCGAC CCAGCGCCAA TGATCTGCTC TTTCACCCAT TGCTTTTCGA GGTGCACTCC 
CTCAAACTGC TGACTGCCCA TTGCCTGGTC TTCTCGCCCG CCAACCGCAC TATGTTCTCG 
GAAACCGCCT TCGACGGCCT CATGCAGCGA TACTACCAGC CGGACGTGGT TATGGCCCAG 
CTGCGGCTGG CCGGCGGCCA GGAGCGGCAG TACCGACTCG CAGACGTTTC AGGCGCCGAT 
AAGCTGGAAA AGTTCGTCGA GGACGTGAAG TATGGTGTGT ATCCGCTAAT TACGTACAGC 
GGCAAGAAGC CGCCCAACTT CCGTTCCCGG GCCGCCTCCC CGGAACGGGC TGACTCCGTA 
AAATCGGCCA CGCCGGAGCC TGTGGACACG GAGTCGCGGC GCATCGTTAA CATGATGTGC 
AGCGTTAAAA TCAAAGAGGA TAGCAATGAC ATAACCATGA CAATACTGCT GCGCATGGAC 
GACAAGATGA ACCGCCAACT GACATGCCAG GTGAACGAGA ACGATACGGC GGCGGATCTC 
ACCAGCGAAC TGGTGCGCCT CGGTTTCGTC CACCTCGACG ACCAGGACAA GATCCAGGTG 
CTGCTGGAGG AAACGCTTAA AGCGGGCGTT ATGAGCGACG GTGCCGGGGC AGAGAGCTCC 
GGTGCGGGGG TGACGACCAC GGCGACCATG GCGGCCTTGG AGCAGCTGGA GCGCAACTGG 
TCGATATCCT CGGATGCAGA CAAGCAGGGC ACTGCCGTGA TGTATGTACC GCAGGAGCAG 
CAGAACGCGG ACGGGGACGT CGACGTAGAA CACTCGGGCA CGACGAGCAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Madm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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