Sym cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sym
Entrez Gene ID	40709
Full Name	Symplekin
Synonyms	CG2097,Dmel\CG2097,Symp,dSymp
General protein information	Preferred Names Symplekin Names CG2097 gene product from transcript CG2097-RA CG2097-PA Sym-PA symplekin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83C1-83C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_141323.2	NP_649580.1	symplekin

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SYMPK	NP_001193842.1
Danio rerio (zebrafish)	si:dkeyp-117b8.1	XP_005165630.1
Homo sapiens (human)	SYMPK	NP_004810.2
Caenorhabditis elegans (roundworm)	symk-1	NP_505210.2
Mus musculus (house mouse)	Sympk	NP_080881.2
Rattus norvegicus (Norway rat)	Sympk	NP_001094300.1
Canis lupus familiaris (dog)	SYMPK	XP_533638.2
Drosophila melanogaster (fruit fly)	Sym	NP_649580.1
Xenopus tropicalis (tropical clawed frog)	sympk	NP_989259.2
Pan troglodytes (chimpanzee)	SYMPK	XP_001166898.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002618	XP_312315.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors propose a model in which the core cleavage complex formation is mediated by CPSF73, CPSF100, and Symplekin C-termini, and the N-terminal region of Symplekin facilitates cotranscriptional 3' end processing of histone mRNAs.
Title: In vivo characterization of the Drosophila mRNA 3' end processing core cleavage complex.

This localization depends on the RNA binding protein ypsilon schachtel. CPSF-73 and a number of mRNAs are localized at this same site, suggesting that Symplekin participates in cytoplasmic polyadenylation at tricellular junctions.
Title: Drosophila Symplekin localizes dynamically to the histone locus body and tricellular junctions.

Together, these data support the conclusion that the Symplekin HEAT domain serves as a scaffold for protein-protein interactions essential to the mRNA maturation process.
Title: Crystal structure of the HEAT domain from the Pre-mRNA processing factor Symplekin.

The following Sym gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sym cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03991	NM_141323.2 Latest version!	Drosophila melanogaster symplekin (Sym), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sym ( NM_141323.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649580.1 Drosophila melanogaster symplekin (Sym), mRNA.

Price & Availability↑

CloneID	OFa03991
Clone ID Related Accession (Same CDS sequence)	NM_141323.2
Accession Version	NM_141323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	symplekin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141323.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAGCA TAATTGGACG CAGCCAGTTT GTCTCGGAGA CGGCCAATCT GTTCACAGAC 
GAGAAGACAG CTACGGCAAG AGCTAAGGTG GTCGATTGGT GCAATGAGCT GGTCATCGCA 
TCGCCTTCGA CAAAGTGCGA ACTGCTGGCT AAGGTGCAGG AGACTGTGCT TGGATCGTGC 
GCGGAGCTGG CCGAAGAATT CCTGGAGTCC GTGCTGTCTT TGGCCCACGA CTCAAACATG 
GAGGTTCGCA AGCAGGTAGT CGCCTTCGTA GAGCAAGTTT GTAAAGTGAA GGTGGAGTTA 
CTGCCCCATG TCATTAACGT CGTGTCTATG CTGCTTAGGG ATAACTCGGC TCAGGTAATC 
AAAAGAGTGA TCCAAGCCTG CGGTAGCATC TACAAGAATG GATTGCAGTA CTTGTGCAGC 
CTCATGGAGC CCGGCGACAG TGCGGAGCAG GCGTGGAACA TCCTCAGCTT AATCAAGGCG 
CAAATACTGG ACATGATCGA CAATGAAAAC GACGGCATAC GTACAAATGC CATTAAGTTC 
TTGGAGGGCG TCGTGGTTCT GCAGAGCTTT GCTGACGAGG ACAGTCTGAA ACGAGATGGA 
GACTTCTCGC TGGCAGATGT CCCCGATCAC TGCACACTAT TCCGCCGTGA AAAGTTGCAA 
GAGGAGGGCA ACAATATTTT AGATATCTTG CTTCAGTTCC ACGGAACAAC GCATATTTCC 
TCAGTGAACT TGATTGCCTG CACAAGCAGC TTGTGTACAA TTGCCAAAAT GCGACCCATT 
TTCATGGGCG CCGTGGTGGA AGCCTTTAAG CAGCTGAATG CCAACCTGCC GCCCACCCTC 
ACTGACTCGC AAGTAAGCTC CGTGCGTAAG AGCCTTAAGA TGCAGTTGCA GACATTGCTG 
AAGAACCGCG GTGCCTTTGA GTTTGCAAGC ACTATCCGAG GAATGTTAGT TGATCTGGGG 
TCCTCCACAA ATGAGATCCA GAAGCTTATT CCCAAAATGG ACAAACAGGA AATGGCTCGT 
AGACAAAAAC GCATCCTCGA AAATGCTGCG CAAAGTCTTG CAAAGCGTGC GCGCTTGGCC 
TGTGAGCAGC AGGATCAGCA GCAGCGAGAA ATGGAGCTAG ACACTGAGGA ATTGGAACGA 
CAGAAACAGA AGTCTACTCG AGTTAACGAA AAGTTTCTGG CAGAGCATTT CCGTAATCCT 
GAAACTGTTG TGACCCTGGT GCTGGAGTTC CTGCCCAGTC TGCCCACTGA GGTGCCACAA 
AAGTTCCTTC AGGAATATAC ACCCATTCGC GAAATGTCTA TTCAGCAGCA GGTAACCAAT 
ATCTCCAGAT TTTTTGGCGA ACAACTATCT GAAAAGCGCC TAGGCCCAGG TGCCGCAACC 
TTCAGTCGAG AGCCGCCAAT GCGGGTTAAG AAAGTGCAAG CGATTGAATC AACACTAACT 
GCAATGGAAG TGGACGAGGA TGCTGTTCAA AAACTGAGTG AGGAAGAGTT TCAGCGTAAG 
GAGGAAGCAA CCAAGAAGCT GCGCGAGACC ATGGAGCGCG CCAAGGGTGA GCAGACTGTT 
ATTGAAAAGA TGAAGGAGCG CGCCAAGACG CTGAAGCTGC AGGAGATCAC CAAGCCTCTG 
CCACGCAACT TAAAAGAGAA GTTCCTAACT GATGCAGTGC GTCGCATCCT CAACTCGGAG 
CGGCAATGCA TTAAGGGAGG AGTGTCATCA AAGCGCCGTA AGCTGGTCAC CGTGATTGCT 
GCCACCTTTC CCGATAACGT ACGTTACGGC ATCATGGAGT TCATCCTGGA GGATATAAAA 
CAGCGTATCG ATTTGGCATT TTCCTGGCTC TTTGAGGAGT ACTCTCTGCT GCAAGGATTT 
ACAAGGCACA CTTATGTTAA GACAGAGAAC AGACCCGATC ACGCCTACAA CGAGTTGCTG 
AATAAGCTTA TCTTCGGCAT TGGAGAGCGT TGCGATCACA AGGATAAGAT TATTTTGATT 
CGTCGAGTAT ATCTAGAAGC ACCCATTCTT CCGGAAGTCT CAATTGGACA TCTAGTTCAA 
TTAAGCCTGG ACGACGAGTT CTCCCAGCAC GGCCTGGAGC TAATCAAGGA CCTTGCAGTG 
CTTAGGCCGC CGCGAAAGAA TCGTTTCGTT AGAGTGTTGC TCAACTTTTC TGTGCATGAG 
CGACTGGATC TGCGGGATCT GGCACAGGCC CACCTGGTCA GTCTGTACCA TGTACACAAA 
ATACTACCGG CTCGAATAGA CGAATTCGCT CTGGAGTGGC TTAAATTTAT CGAACAGGAA 
TCTCCGCCGG CAGCTGTTTT CTCGCAGGAC TTTGGTCGAC CAACCGAGGA GCCTGATTGG 
CGGGAAGACA CTACCAAGGT GTGCTTTGGT TTGGCCTTTA CCCTGCTGCC CTATAAACCG 
GAAGTTTACT TACAGCAAAT TTGTCAAGTG TTTGTTTCTA CATCTGCCGA ACTGAAACGC 
ACGATCTTGC GCAGTCTAGA TATTCCCATA AAAAAGATGG GTGTAGAAAG CCCGACGTTG 
CTGCAACTAA TCGAGGATTG TCCTAAGGGT ATGGAGACCT TAGTGATCCG TATAATTTAC 
ATACTGACAG AGCGAGTGCC ATCCCCCCAT GAAGAATTGG TTCGGCGGGT TCGGGATCTC 
TACCAGAACA AGGTCAAGGA TGTGCGCGTG ATGATACCCG TGCTAAGTGG CTTAACCCGA 
TCCGAGCTAA TTTCCGTTCT GCCGAAGCTG ATTAAACTTA ATCCCGCTGT GGTCAAGGAG 
GTTTTCAATC GATTGCTTGG TATCGGTGCC GAATTCGCGC ATCAGACGAT GGCCATGACC 
CCCACTGATA TTCTCGTGGC TTTGCACACC ATCGATACGA GTGTCTGCGA TATTAAAGCC 
ATCGTTAAGG CTACATCCCT ATGTTTGGCT GAAAGAGATC TGTACACACA GGAGGTACTT 
ATGGCTGTGC TGCAACAGCT TGTAGAGGTT ACTCCGCTGC CTACTCTGAT GATGCGCACA 
ACAATTCAGA GTCTAACTCT GTACCCGCGT CTGGCAAACT TTGTAATGAA CTTGTTGCAG 
CGTCTGATAA TCAAGCAGGT CTGGCGGCAA AAAGTAATCT GGGAGGGCTT TTTAAAGACC 
GTGCAGCGCC TGAAACCGCA GTCAATGCCG ATCCTGCTGC ACCTTCCTCC CGCCCAGCTG 
GTGGACGCAC TGCAGCAGTG CCCCGATTTA AGGCCAGCAT TGTCGGAATA CGCGGAGAGC 
ATGCAGGATG AACCAATGAA TGGTAGTGGT ATAACCCAGC AGGTTCTGGA CATCATCTCC 
GGGAAATCAG TTGATGTGTT CGTGACGGAC GAAAGCGGCG GTTACATCAG TGCCGAGCAT 
ATTAAAAAAG AGGCACCGGA TCCATCAGAA ATTAGTGTCA TTTCCACAGT GCCCGTACTG 
ACGTCTCTAG TACCACTACC AGTTCCTCCC CCCATAGGCA GTGATCTTAA CCAGCCCCTG 
CCGCCTGGCG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649580.1
CDS	349..3846
Translation	MDSIIGRSQFVSETANLFTDEKTATARAKVVDWCNELVIASPSTKCELLAKVQETVLGSCAELAEEFLESVLSLAHDSNMEVRKQVVAFVEQVCKVKVELLPHVINVVSMLLRDNSAQVIKRVIQACGSIYKNGLQYLCSLMEPGDSAEQAWNILSLIKAQILDMIDNENDGIRTNAIKFLEGVVVLQSFADEDSLKRDGDFSLADVPDHCTLFRREKLQEEGNNILDILLQFHGTTHISSVNLIACTSSLCTIAKMRPIFMGAVVEAFKQLNANLPPTLTDSQVSSVRKSLKMQLQTLLKNRGAFEFASTIRGMLVDLGSSTNEIQKLIPKMDKQEMARRQKRILENAAQSLAKRARLACEQQDQQQREMELDTEELERQKQKSTRVNEKFLAEHFRNPETVVTLVLEFLPSLPTEVPQKFLQEYTPIREMSIQQQVTNISRFFGEQLSEKRLGPGAATFSREPPMRVKKVQAIESTLTAMEVDEDAVQKLSEEEFQRKEEATKKLRETMERAKGEQTVIEKMKERAKTLKLQEITKPLPRNLKEKFLTDAVRRILNSERQCIKGGVSSKRRKLVTVIAATFPDNVRYGIMEFILEDIKQRIDLAFSWLFEEYSLLQGFTRHTYVKTENRPDHAYNELLNKLIFGIGERCDHKDKIILIRRVYLEAPILPEVSIGHLVQLSLDDEFSQHGLELIKDLAVLRPPRKNRFVRVLLNFSVHERLDLRDLAQAHLVSLYHVHKILPARIDEFALEWLKFIEQESPPAAVFSQDFGRPTEEPDWREDTTKVCFGLAFTLLPYKPEVYLQQICQVFVSTSAELKRTILRSLDIPIKKMGVESPTLLQLIEDCPKGMETLVIRIIYILTERVPSPHEELVRRVRDLYQNKVKDVRVMIPVLSGLTRSELISVLPKLIKLNPAVVKEVFNRLLGIGAEFAHQTMAMTPTDILVALHTIDTSVCDIKAIVKATSLCLAERDLYTQEVLMAVLQQLVEVTPLPTLMMRTTIQSLTLYPRLANFVMNLLQRLIIKQVWRQKVIWEGFLKTVQRLKPQSMPILLHLPPAQLVDALQQCPDLRPALSEYAESMQDEPMNGSGITQQVLDIISGKSVDVFVTDESGGYISAEHIKKEAPDPSEISVISTVPVLTSLVPLPVPPPIGSDLNQPLPPGED

Target ORF information:

RefSeq Version	NM_141323.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster symplekin (Sym), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141323.2

ORF Insert Sequence:

ATGGATAGCA TAATTGGACG CAGCCAGTTT GTCTCGGAGA CGGCCAATCT GTTCACAGAC 
GAGAAGACAG CTACGGCAAG AGCTAAGGTG GTCGATTGGT GCAATGAGCT GGTCATCGCA 
TCGCCTTCGA CAAAGTGCGA ACTGCTGGCT AAGGTGCAGG AGACTGTGCT TGGATCGTGC 
GCGGAGCTGG CCGAAGAATT CCTGGAGTCC GTGCTGTCTT TGGCCCACGA CTCAAACATG 
GAGGTTCGCA AGCAGGTAGT CGCCTTCGTA GAGCAAGTTT GTAAAGTGAA GGTGGAGTTA 
CTGCCCCATG TCATTAACGT CGTGTCTATG CTGCTTAGGG ATAACTCGGC TCAGGTAATC 
AAAAGAGTGA TCCAAGCCTG CGGTAGCATC TACAAGAATG GATTGCAGTA CTTGTGCAGC 
CTCATGGAGC CCGGCGACAG TGCGGAGCAG GCGTGGAACA TCCTCAGCTT AATCAAGGCG 
CAAATACTGG ACATGATCGA CAATGAAAAC GACGGCATAC GTACAAATGC CATTAAGTTC 
TTGGAGGGCG TCGTGGTTCT GCAGAGCTTT GCTGACGAGG ACAGTCTGAA ACGAGATGGA 
GACTTCTCGC TGGCAGATGT CCCCGATCAC TGCACACTAT TCCGCCGTGA AAAGTTGCAA 
GAGGAGGGCA ACAATATTTT AGATATCTTG CTTCAGTTCC ACGGAACAAC GCATATTTCC 
TCAGTGAACT TGATTGCCTG CACAAGCAGC TTGTGTACAA TTGCCAAAAT GCGACCCATT 
TTCATGGGCG CCGTGGTGGA AGCCTTTAAG CAGCTGAATG CCAACCTGCC GCCCACCCTC 
ACTGACTCGC AAGTAAGCTC CGTGCGTAAG AGCCTTAAGA TGCAGTTGCA GACATTGCTG 
AAGAACCGCG GTGCCTTTGA GTTTGCAAGC ACTATCCGAG GAATGTTAGT TGATCTGGGG 
TCCTCCACAA ATGAGATCCA GAAGCTTATT CCCAAAATGG ACAAACAGGA AATGGCTCGT 
AGACAAAAAC GCATCCTCGA AAATGCTGCG CAAAGTCTTG CAAAGCGTGC GCGCTTGGCC 
TGTGAGCAGC AGGATCAGCA GCAGCGAGAA ATGGAGCTAG ACACTGAGGA ATTGGAACGA 
CAGAAACAGA AGTCTACTCG AGTTAACGAA AAGTTTCTGG CAGAGCATTT CCGTAATCCT 
GAAACTGTTG TGACCCTGGT GCTGGAGTTC CTGCCCAGTC TGCCCACTGA GGTGCCACAA 
AAGTTCCTTC AGGAATATAC ACCCATTCGC GAAATGTCTA TTCAGCAGCA GGTAACCAAT 
ATCTCCAGAT TTTTTGGCGA ACAACTATCT GAAAAGCGCC TAGGCCCAGG TGCCGCAACC 
TTCAGTCGAG AGCCGCCAAT GCGGGTTAAG AAAGTGCAAG CGATTGAATC AACACTAACT 
GCAATGGAAG TGGACGAGGA TGCTGTTCAA AAACTGAGTG AGGAAGAGTT TCAGCGTAAG 
GAGGAAGCAA CCAAGAAGCT GCGCGAGACC ATGGAGCGCG CCAAGGGTGA GCAGACTGTT 
ATTGAAAAGA TGAAGGAGCG CGCCAAGACG CTGAAGCTGC AGGAGATCAC CAAGCCTCTG 
CCACGCAACT TAAAAGAGAA GTTCCTAACT GATGCAGTGC GTCGCATCCT CAACTCGGAG 
CGGCAATGCA TTAAGGGAGG AGTGTCATCA AAGCGCCGTA AGCTGGTCAC CGTGATTGCT 
GCCACCTTTC CCGATAACGT ACGTTACGGC ATCATGGAGT TCATCCTGGA GGATATAAAA 
CAGCGTATCG ATTTGGCATT TTCCTGGCTC TTTGAGGAGT ACTCTCTGCT GCAAGGATTT 
ACAAGGCACA CTTATGTTAA GACAGAGAAC AGACCCGATC ACGCCTACAA CGAGTTGCTG 
AATAAGCTTA TCTTCGGCAT TGGAGAGCGT TGCGATCACA AGGATAAGAT TATTTTGATT 
CGTCGAGTAT ATCTAGAAGC ACCCATTCTT CCGGAAGTCT CAATTGGACA TCTAGTTCAA 
TTAAGCCTGG ACGACGAGTT CTCCCAGCAC GGCCTGGAGC TAATCAAGGA CCTTGCAGTG 
CTTAGGCCGC CGCGAAAGAA TCGTTTCGTT AGAGTGTTGC TCAACTTTTC TGTGCATGAG 
CGACTGGATC TGCGGGATCT GGCACAGGCC CACCTGGTCA GTCTGTACCA TGTACACAAA 
ATACTACCGG CTCGAATAGA CGAATTCGCT CTGGAGTGGC TTAAATTTAT CGAACAGGAA 
TCTCCGCCGG CAGCTGTTTT CTCGCAGGAC TTTGGTCGAC CAACCGAGGA GCCTGATTGG 
CGGGAAGACA CTACCAAGGT GTGCTTTGGT TTGGCCTTTA CCCTGCTGCC CTATAAACCG 
GAAGTTTACT TACAGCAAAT TTGTCAAGTG TTTGTTTCTA CATCTGCCGA ACTGAAACGC 
ACGATCTTGC GCAGTCTAGA TATTCCCATA AAAAAGATGG GTGTAGAAAG CCCGACGTTG 
CTGCAACTAA TCGAGGATTG TCCTAAGGGT ATGGAGACCT TAGTGATCCG TATAATTTAC 
ATACTGACAG AGCGAGTGCC ATCCCCCCAT GAAGAATTGG TTCGGCGGGT TCGGGATCTC 
TACCAGAACA AGGTCAAGGA TGTGCGCGTG ATGATACCCG TGCTAAGTGG CTTAACCCGA 
TCCGAGCTAA TTTCCGTTCT GCCGAAGCTG ATTAAACTTA ATCCCGCTGT GGTCAAGGAG 
GTTTTCAATC GATTGCTTGG TATCGGTGCC GAATTCGCGC ATCAGACGAT GGCCATGACC 
CCCACTGATA TTCTCGTGGC TTTGCACACC ATCGATACGA GTGTCTGCGA TATTAAAGCC 
ATCGTTAAGG CTACATCCCT ATGTTTGGCT GAAAGAGATC TGTACACACA GGAGGTACTT 
ATGGCTGTGC TGCAACAGCT TGTAGAGGTT ACTCCGCTGC CTACTCTGAT GATGCGCACA 
ACAATTCAGA GTCTAACTCT GTACCCGCGT CTGGCAAACT TTGTAATGAA CTTGTTGCAG 
CGTCTGATAA TCAAGCAGGT CTGGCGGCAA AAAGTAATCT GGGAGGGCTT TTTAAAGACC 
GTGCAGCGCC TGAAACCGCA GTCAATGCCG ATCCTGCTGC ACCTTCCTCC CGCCCAGCTG 
GTGGACGCAC TGCAGCAGTG CCCCGATTTA AGGCCAGCAT TGTCGGAATA CGCGGAGAGC 
ATGCAGGATG AACCAATGAA TGGTAGTGGT ATAACCCAGC AGGTTCTGGA CATCATCTCC 
GGGAAATCAG TTGATGTGTT CGTGACGGAC GAAAGCGGCG GTTACATCAG TGCCGAGCAT 
ATTAAAAAAG AGGCACCGGA TCCATCAGAA ATTAGTGTCA TTTCCACAGT GCCCGTACTG 
ACGTCTCTAG TACCACTACC AGTTCCTCCC CCCATAGGCA GTGATCTTAA CCAGCCCCTG 
CCGCCTGGCG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sym cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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