PEK cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PEK
Entrez Gene ID	40653
Full Name	pancreatic eIF-2alpha kinase
Synonyms	CG2087,DPERK,DmPEK,Dmel\CG2087,DpERK,PERK,Pek,Perk,dPERK,dPerk,pERK,pek,perk
General protein information	Preferred Names pancreatic eIF-2alpha kinase Names CG2087 gene product from transcript CG2087-RA CG2087-PA CG2087-PB CG2087-PC PEK-PA PEK-PB PEK-PC PERK pancreatic eIF2alpha kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83A4-83A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275353.1	NP_001262282.1	pancreatic eIF-2alpha kinase, isoform B
NM_001275354.1	NP_001262283.1	pancreatic eIF-2alpha kinase, isoform C
NM_141281.3	NP_649538.1	pancreatic eIF-2alpha kinase, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show that the PKR-like ER kinase (PERK) integrates both cell-autonomous and non-autonomous endoplasmic reticulum stress stimuli to induce intestinal stem cell proliferation.
Title: PERK Limits Drosophila Lifespan by Promoting Intestinal Stem Cell Proliferation in Response to ER Stress.

The following PEK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PEK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03928	NM_141281.3 Latest version!	Drosophila melanogaster pancreatic eIF-2alpha kinase (PEK), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa07709	NM_001275353.1 Latest version!	Drosophila melanogaster pancreatic eIF-2alpha kinase (PEK), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa07710	NM_001275354.1 Latest version!	Drosophila melanogaster pancreatic eIF-2alpha kinase (PEK), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PEK ( NM_141281.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649538.1 Drosophila melanogaster pancreatic eIF-2alpha kinase (PEK), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03928
Clone ID Related Accession (Same CDS sequence)	NM_141281.3
Accession Version	NM_141281.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pancreatic eIF-2alpha kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141281.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGACG ACCTGGATGG CATCGTGAGG CATCGTCGCC GGAGCCTGAG CTTCCTGCAG 
ATCGTCACGC TCACTATGGC CGGCCTGGTG GCTTTCGATC CCGCCCAGGT GCTAGCTGGG 
CACCCAACTA CTGATTCGGA GCTCCAGACT GCCGGATCTC CCCGTCCCCC CGGGCTCGAA 
CACTGCGTAG ATCAGGAGGA GCGTCGCGTT GCAAGAAGAC TCCTATACAT TTCCACGCTT 
GATGGTCGAC TGAGCGCTCT GGACATCGCC AAGTCCGGCA AACTGCGCTG GAGTGTGCCC 
ACCGGACCGG GGCCACTAAT CTCGTCCAGC ATCCATCGAT TGGAGCTGAC CAACAATGGG 
CAGTTCGTAC GCATGATTCC CTCTTTGAGC GGCGGCATCT ACAAGTTCGA CGGGGACTCC 
ATCGATCCCA TTCCAATCAC GGCGGAGCAC CTACTCAGCT CGAGCGCCAA ATTCAGCGAT 
GATCTGGTTA TATCCGGAGG CAAGGAGACA CGCTCATACG GTGTTTCCGT TCGCACTGGC 
CAGCTGCTGT ATGAATGTTC TCTAAACGGA TGTGTAAACT CTACGGAGGA AGGGCTTGCA 
ATCGACGACA CCATTAGGGA GCCCGATGAG GAGGATCAGC TGGAGGACGG AGAACAGTTA 
AGGGACGAGG CCGGCTACAT AGTGCGCCAT GATCCTCTCC TTGACGACGT TATTATCGTC 
CGCCGCCAGA CACAAACGGT TCGGGCCGTG GAATCCCGGA CCGGAGTGGA GCGTTGGAAC 
TTTAGCGTAG GTCAGCATGA GTTGGATCTG GTGAGACCTT CCGAGTGCCA GCTACAGCCG 
CGCGATGAGC TGGAGCTGGC TGTTTTGGAT GTGGATATAA AGGTGGTAGT GCCCGAGGGA 
ATTATCTGCG CCTTCAGCAA AAGTGAGCCA CAGACTATGC TGTGGAAGTA CAAGTTTGAT 
CACCCAATTG TGAGTGCCTG GAATACAAAT GCCGACGACG AATTGCAGCC TATTGATTTG 
TTTAGCTCGG CCCAGTGGCT TTGGGACCAG GACGAAAATG ACACGGAGCT GCCCAATGCG 
CCACAAAGCC CGCCATCCAT CTACTTGGGC ATGTACGATA AACAGTTGTA TATTCAGGAG 
TCAATACGTC TACGCCAGGA GATTATGGAC CAGACCAAGG TCTATCAGCA GCTTACAGGC 
GACACCAGCC TGATGCCCAG GATTCCCTGG AAGCCGATAT CCGCCAGTAG CAAATCGCTG 
GTAATCTTCC GGAAAGACCA GGAGGACCCT GAAATGATTG CTGAGGGAGC TGTGGCACAA 
GGAGGGGAAC TGGTGCCCTA TGATGATGAA AACTTCGCTG TGGCAGCGCA GTCCGTATTG 
AATGCATCGG AGTTTGTCAA TGGCAATGGT TTTTACTTCT ACACCACTGG CGATCTGAAC 
GGCCCGCAAG AGTGCAGTAC GCAGAATAAT CCAACTGACC TTCCAGCAAT CACAGCTCCA 
ACATCGCCTA CCAATGCCAC ATCTGAGGGA ACGGAAGCTA CTGGAAACCA TAGCGTCAAC 
GACGATTTGG GCTTTAGTTT GGATGACATT GATGCGCCTG TAAAGGTTGT GATATTGTCG 
TTGTGGTTCT GGTGGAAGGA AATCGTGGTC ATAGCATTTA CGTCGGCTGT GATTCTAAAT 
ATTTTCATGG GCCAACGAAA CCAGCGCGTG GAGCGAGAGT ATCTGGTCAT TGAACGTCAT 
GTGCCTGTTC AAACGGCCAT CGAGGCCACC GAAGCATCCA CACAGGCTTT ACTAGGTCCA 
GTGGTGCCGA TGCAGCGACC TGGAAATCGT TTTAGCTTCC CACCTGGACA AGCAAATCAG 
CGCACCATTT CGGAGTCTAC AACTCATTCA GGCGAGCATT ACACTTCCCG CTTTCAGTCC 
GACTTCGAGT TGATGCAGTG CCTTGGTCGC GGTGGTTTCG GAGTGGTCTT CGAAGCTAAG 
AACAAGCTGG ATGAGAACCG ATACGCCATT AAGCGCATAA CGCTACCCAA TAAGGAATCT 
TCAAGACAGC GAGTCCTTCG CGAGGCTCGA ACTTTGGCTA GTTGTGAGCA CCACAACATC 
GTGCGATACT TCCATTCCTG GACCGAGACA CCGCCGACTG GTTGGCAGGA AGAAGAGGAT 
CGCAAGCTGC TTGCCCACGA GCTGTCCACC TCTATCCAGA TAGAGACGCC GGACGATAGT 
ACAATGCCTT CGTTGACGGA GCAGTTGAAG GAGAAACGCC AACAGCAGCT CTTGTCCTGG 
GTTTCGGATG CAGCCAATAG CACCGCTTGT AGTCACGACT TTCATCTACC AGGCGAGTCA 
TCACTCAAGA ACATACGCGA GGAGTACGAC TACGATGAGG AAGAGGACTC GCTCATTGAA 
TTCCGCAGCG AGTCGCAGTC AGCCGCTTTA CGGGCAGAGG AGGAGGATGA TACCGACGAC 
GACTATGAAG AGGATGAAGA GCAGCAGGGA GACCATGAGA AACGACATCG CAGCTCCGTG 
TCCATTGACA TCCATTCGGC CTCGTTTGAC CTTAAGAACA TAAACTACTC ACAGCACCAA 
CTGGTCTCCA ATTCGTTTCA AATCGAATCT GTGCGCCCCA AGAGCAGTGG AAGTGATGAT 
GCGAATGACG ATAATAAAGC TAGAAGGAAG CCCCTTACCT TGGCACTGGC CCAGAATCAC 
AATAACAACC AAAACGGGAG CCAACCTACG CCCTCCAGTG CCACGATTCT GAACGGAACG 
GTGGCCAAGC CCAGCAAGGT GTACCTCTAC ATCCAGATGC AGCTATGCCG CAAGGAGAGT 
CTGCGCGACT GGTTAAGGGA CAACCGGTCG GAAACAAGGG CTGCCCACAT TGGTGACATT 
TTTCACCAGA TCGTTGATGC CGTTGACTAT GTACATTTGA AGGGTCTGAT TCATCGGGAC 
CTGAAACCAA GCAACATATT CTTCTCGCAG GATGGACAGA TAAAGATTGG AGACTTCGGG 
CTCGTCACCG ACATGGCCGA TATCCCCAAC CTGGTGGCCA AGTGTGGGGA CCAGAGTGGC 
CTGCCCTCCT GTGCCCGCCA CACCCAGCAG GTGGGCACCC ACTTATACAT GTCGCCGGAG 
CAGCTTTTGG GCCAGCACTA CGACTACAAG GTGGACATCT ATTCACTGGG ACTGATCTTC 
TTCGAGCTGC ACGTGTACTT CAGCACTGAA ATGGAGCGGA TCAAGACACT GCGCAGTCTT 
CGGGACGGCC AGTATCCCAA GGATTTCGCT GTCAATTACC CGCAGCAGTA CGACTTGCTC 
CAGCAAATGC TATCCGCCCA GCCAGAGCAA CGCCCGCAGA CCAAGCAGTT GAAAAGCCAG 
CTGCGCAACA TTCTGCAGCT ACCTCATCTC CTCTCGGAAG GACAAAGCGA GCAGGCGGAG 
CTGGCGGAGC GGGCCAGGCG CCTGAGTCGC AGCCGCACCT TCAGCAGCTC CAGTGAGCCG 
CATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649538.1
CDS	322..3810
Translation	MQDDLDGIVRHRRRSLSFLQIVTLTMAGLVAFDPAQVLAGHPTTDSELQTAGSPRPPGLEHCVDQEERRVARRLLYISTLDGRLSALDIAKSGKLRWSVPTGPGPLISSSIHRLELTNNGQFVRMIPSLSGGIYKFDGDSIDPIPITAEHLLSSSAKFSDDLVISGGKETRSYGVSVRTGQLLYECSLNGCVNSTEEGLAIDDTIREPDEEDQLEDGEQLRDEAGYIVRHDPLLDDVIIVRRQTQTVRAVESRTGVERWNFSVGQHELDLVRPSECQLQPRDELELAVLDVDIKVVVPEGIICAFSKSEPQTMLWKYKFDHPIVSAWNTNADDELQPIDLFSSAQWLWDQDENDTELPNAPQSPPSIYLGMYDKQLYIQESIRLRQEIMDQTKVYQQLTGDTSLMPRIPWKPISASSKSLVIFRKDQEDPEMIAEGAVAQGGELVPYDDENFAVAAQSVLNASEFVNGNGFYFYTTGDLNGPQECSTQNNPTDLPAITAPTSPTNATSEGTEATGNHSVNDDLGFSLDDIDAPVKVVILSLWFWWKEIVVIAFTSAVILNIFMGQRNQRVEREYLVIERHVPVQTAIEATEASTQALLGPVVPMQRPGNRFSFPPGQANQRTISESTTHSGEHYTSRFQSDFELMQCLGRGGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRVLREARTLASCEHHNIVRYFHSWTETPPTGWQEEEDRKLLAHELSTSIQIETPDDSTMPSLTEQLKEKRQQQLLSWVSDAANSTACSHDFHLPGESSLKNIREEYDYDEEEDSLIEFRSESQSAALRAEEEDDTDDDYEEDEEQQGDHEKRHRSSVSIDIHSASFDLKNINYSQHQLVSNSFQIESVRPKSSGSDDANDDNKARRKPLTLALAQNHNNNQNGSQPTPSSATILNGTVAKPSKVYLYIQMQLCRKESLRDWLRDNRSETRAAHIGDIFHQIVDAVDYVHLKGLIHRDLKPSNIFFSQDGQIKIGDFGLVTDMADIPNLVAKCGDQSGLPSCARHTQQVGTHLYMSPEQLLGQHYDYKVDIYSLGLIFFELHVYFSTEMERIKTLRSLRDGQYPKDFAVNYPQQYDLLQQMLSAQPEQRPQTKQLKSQLRNILQLPHLLSEGQSEQAELAERARRLSRSRTFSSSSEPHQ

Target ORF information:

RefSeq Version	NM_141281.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pancreatic eIF-2alpha kinase (PEK), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141281.3

ORF Insert Sequence:

ATGCAGGACG ACCTGGATGG CATCGTGAGG CATCGTCGCC GGAGCCTGAG CTTCCTGCAG 
ATCGTCACGC TCACTATGGC CGGCCTGGTG GCTTTCGATC CCGCCCAGGT GCTAGCTGGG 
CACCCAACTA CTGATTCGGA GCTCCAGACT GCCGGATCTC CCCGTCCCCC CGGGCTCGAA 
CACTGCGTAG ATCAGGAGGA GCGTCGCGTT GCAAGAAGAC TCCTATACAT TTCCACGCTT 
GATGGTCGAC TGAGCGCTCT GGACATCGCC AAGTCCGGCA AACTGCGCTG GAGTGTGCCC 
ACCGGACCGG GGCCACTAAT CTCGTCCAGC ATCCATCGAT TGGAGCTGAC CAACAATGGG 
CAGTTCGTAC GCATGATTCC CTCTTTGAGC GGCGGCATCT ACAAGTTCGA CGGGGACTCC 
ATCGATCCCA TTCCAATCAC GGCGGAGCAC CTACTCAGCT CGAGCGCCAA ATTCAGCGAT 
GATCTGGTTA TATCCGGAGG CAAGGAGACA CGCTCATACG GTGTTTCCGT TCGCACTGGC 
CAGCTGCTGT ATGAATGTTC TCTAAACGGA TGTGTAAACT CTACGGAGGA AGGGCTTGCA 
ATCGACGACA CCATTAGGGA GCCCGATGAG GAGGATCAGC TGGAGGACGG AGAACAGTTA 
AGGGACGAGG CCGGCTACAT AGTGCGCCAT GATCCTCTCC TTGACGACGT TATTATCGTC 
CGCCGCCAGA CACAAACGGT TCGGGCCGTG GAATCCCGGA CCGGAGTGGA GCGTTGGAAC 
TTTAGCGTAG GTCAGCATGA GTTGGATCTG GTGAGACCTT CCGAGTGCCA GCTACAGCCG 
CGCGATGAGC TGGAGCTGGC TGTTTTGGAT GTGGATATAA AGGTGGTAGT GCCCGAGGGA 
ATTATCTGCG CCTTCAGCAA AAGTGAGCCA CAGACTATGC TGTGGAAGTA CAAGTTTGAT 
CACCCAATTG TGAGTGCCTG GAATACAAAT GCCGACGACG AATTGCAGCC TATTGATTTG 
TTTAGCTCGG CCCAGTGGCT TTGGGACCAG GACGAAAATG ACACGGAGCT GCCCAATGCG 
CCACAAAGCC CGCCATCCAT CTACTTGGGC ATGTACGATA AACAGTTGTA TATTCAGGAG 
TCAATACGTC TACGCCAGGA GATTATGGAC CAGACCAAGG TCTATCAGCA GCTTACAGGC 
GACACCAGCC TGATGCCCAG GATTCCCTGG AAGCCGATAT CCGCCAGTAG CAAATCGCTG 
GTAATCTTCC GGAAAGACCA GGAGGACCCT GAAATGATTG CTGAGGGAGC TGTGGCACAA 
GGAGGGGAAC TGGTGCCCTA TGATGATGAA AACTTCGCTG TGGCAGCGCA GTCCGTATTG 
AATGCATCGG AGTTTGTCAA TGGCAATGGT TTTTACTTCT ACACCACTGG CGATCTGAAC 
GGCCCGCAAG AGTGCAGTAC GCAGAATAAT CCAACTGACC TTCCAGCAAT CACAGCTCCA 
ACATCGCCTA CCAATGCCAC ATCTGAGGGA ACGGAAGCTA CTGGAAACCA TAGCGTCAAC 
GACGATTTGG GCTTTAGTTT GGATGACATT GATGCGCCTG TAAAGGTTGT GATATTGTCG 
TTGTGGTTCT GGTGGAAGGA AATCGTGGTC ATAGCATTTA CGTCGGCTGT GATTCTAAAT 
ATTTTCATGG GCCAACGAAA CCAGCGCGTG GAGCGAGAGT ATCTGGTCAT TGAACGTCAT 
GTGCCTGTTC AAACGGCCAT CGAGGCCACC GAAGCATCCA CACAGGCTTT ACTAGGTCCA 
GTGGTGCCGA TGCAGCGACC TGGAAATCGT TTTAGCTTCC CACCTGGACA AGCAAATCAG 
CGCACCATTT CGGAGTCTAC AACTCATTCA GGCGAGCATT ACACTTCCCG CTTTCAGTCC 
GACTTCGAGT TGATGCAGTG CCTTGGTCGC GGTGGTTTCG GAGTGGTCTT CGAAGCTAAG 
AACAAGCTGG ATGAGAACCG ATACGCCATT AAGCGCATAA CGCTACCCAA TAAGGAATCT 
TCAAGACAGC GAGTCCTTCG CGAGGCTCGA ACTTTGGCTA GTTGTGAGCA CCACAACATC 
GTGCGATACT TCCATTCCTG GACCGAGACA CCGCCGACTG GTTGGCAGGA AGAAGAGGAT 
CGCAAGCTGC TTGCCCACGA GCTGTCCACC TCTATCCAGA TAGAGACGCC GGACGATAGT 
ACAATGCCTT CGTTGACGGA GCAGTTGAAG GAGAAACGCC AACAGCAGCT CTTGTCCTGG 
GTTTCGGATG CAGCCAATAG CACCGCTTGT AGTCACGACT TTCATCTACC AGGCGAGTCA 
TCACTCAAGA ACATACGCGA GGAGTACGAC TACGATGAGG AAGAGGACTC GCTCATTGAA 
TTCCGCAGCG AGTCGCAGTC AGCCGCTTTA CGGGCAGAGG AGGAGGATGA TACCGACGAC 
GACTATGAAG AGGATGAAGA GCAGCAGGGA GACCATGAGA AACGACATCG CAGCTCCGTG 
TCCATTGACA TCCATTCGGC CTCGTTTGAC CTTAAGAACA TAAACTACTC ACAGCACCAA 
CTGGTCTCCA ATTCGTTTCA AATCGAATCT GTGCGCCCCA AGAGCAGTGG AAGTGATGAT 
GCGAATGACG ATAATAAAGC TAGAAGGAAG CCCCTTACCT TGGCACTGGC CCAGAATCAC 
AATAACAACC AAAACGGGAG CCAACCTACG CCCTCCAGTG CCACGATTCT GAACGGAACG 
GTGGCCAAGC CCAGCAAGGT GTACCTCTAC ATCCAGATGC AGCTATGCCG CAAGGAGAGT 
CTGCGCGACT GGTTAAGGGA CAACCGGTCG GAAACAAGGG CTGCCCACAT TGGTGACATT 
TTTCACCAGA TCGTTGATGC CGTTGACTAT GTACATTTGA AGGGTCTGAT TCATCGGGAC 
CTGAAACCAA GCAACATATT CTTCTCGCAG GATGGACAGA TAAAGATTGG AGACTTCGGG 
CTCGTCACCG ACATGGCCGA TATCCCCAAC CTGGTGGCCA AGTGTGGGGA CCAGAGTGGC 
CTGCCCTCCT GTGCCCGCCA CACCCAGCAG GTGGGCACCC ACTTATACAT GTCGCCGGAG 
CAGCTTTTGG GCCAGCACTA CGACTACAAG GTGGACATCT ATTCACTGGG ACTGATCTTC 
TTCGAGCTGC ACGTGTACTT CAGCACTGAA ATGGAGCGGA TCAAGACACT GCGCAGTCTT 
CGGGACGGCC AGTATCCCAA GGATTTCGCT GTCAATTACC CGCAGCAGTA CGACTTGCTC 
CAGCAAATGC TATCCGCCCA GCCAGAGCAA CGCCCGCAGA CCAAGCAGTT GAAAAGCCAG 
CTGCGCAACA TTCTGCAGCT ACCTCATCTC CTCTCGGAAG GACAAAGCGA GCAGGCGGAG 
CTGGCGGAGC GGGCCAGGCG CCTGAGTCGC AGCCGCACCT TCAGCAGCTC CAGTGAGCCG 
CATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEK ( NM_001275353.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262282.1 Drosophila melanogaster pancreatic eIF-2alpha kinase (PEK), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07709
Clone ID Related Accession (Same CDS sequence)	NM_001275353.1
Accession Version	NM_001275353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pancreatic eIF-2alpha kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGCAC CTTGGCCAAC TATGAAAACC CGTAATCAGT GGACGCGCTT ATCAAGCATG 
CGACTCCTAT ACATTTCCAC GCTTGATGGT CGACTGAGCG CTCTGGACAT CGCCAAGTCC 
GGCAAACTGC GCTGGAGTGT GCCCACCGGA CCGGGGCCAC TAATCTCGTC CAGCATCCAT 
CGATTGGAGC TGACCAACAA TGGGCAGTTC GTACGCATGA TTCCCTCTTT GAGCGGCGGC 
ATCTACAAGT TCGACGGGGA CTCCATCGAT CCCATTCCAA TCACGGCGGA GCACCTACTC 
AGCTCGAGCG CCAAATTCAG CGATGATCTG GTTATATCCG GAGGCAAGGA GACACGCTCA 
TACGGTGTTT CCGTTCGCAC TGGCCAGCTG CTGTATGAAT GTTCTCTAAA CGGATGTGTA 
AACTCTACGG AGGAAGGGCT TGCAATCGAC GACACCATTA GGGAGCCCGA TGAGGAGGAT 
CAGCTGGAGG ACGGAGAACA GTTAAGGGAC GAGGCCGGCT ACATAGTGCG CCATGATCCT 
CTCCTTGACG ACGTTATTAT CGTCCGCCGC CAGACACAAA CGGTTCGGGC CGTGGAATCC 
CGGACCGGAG TGGAGCGTTG GAACTTTAGC GTAGGTCAGC ATGAGTTGGA TCTGGTGAGA 
CCTTCCGAGT GCCAGCTACA GCCGCGCGAT GAGCTGGAGC TGGCTGTTTT GGATGTGGAT 
ATAAAGGTGG TAGTGCCCGA GGGAATTATC TGCGCCTTCA GCAAAAGTGA GCCACAGACT 
ATGCTGTGGA AGTACAAGTT TGATCACCCA ATTGTGAGTG CCTGGAATAC AAATGCCGAC 
GACGAATTGC AGCCTATTGA TTTGTTTAGC TCGGCCCAGT GGCTTTGGGA CCAGGACGAA 
AATGACACGG AGCTGCCCAA TGCGCCACAA AGCCCGCCAT CCATCTACTT GGGCATGTAC 
GATAAACAGT TGTATATTCA GGAGTCAATA CGTCTACGCC AGGAGATTAT GGACCAGACC 
AAGGTCTATC AGCAGCTTAC AGGCGACACC AGCCTGATGC CCAGGATTCC CTGGAAGCCG 
ATATCCGCCA GTAGCAAATC GCTGGTAATC TTCCGGAAAG ACCAGGAGGA CCCTGAAATG 
ATTGCTGAGG GAGCTGTGGC ACAAGGAGGG GAACTGGTGC CCTATGATGA TGAAAACTTC 
GCTGTGGCAG CGCAGTCCGT ATTGAATGCA TCGGAGTTTG TCAATGGCAA TGGTTTTTAC 
TTCTACACCA CTGGCGATCT GAACGGCCCG CAAGAGTGCA GTACGCAGAA TAATCCAACT 
GACCTTCCAG CAATCACAGC TCCAACATCG CCTACCAATG CCACATCTGA GGGAACGGAA 
GCTACTGGAA ACCATAGCGT CAACGACGAT TTGGGCTTTA GTTTGGATGA CATTGATGCG 
CCTGTAAAGG TTGTGATATT GTCGTTGTGG TTCTGGTGGA AGGAAATCGT GGTCATAGCA 
TTTACGTCGG CTGTGATTCT AAATATTTTC ATGGGCCAAC GAAACCAGCG CGTGGAGCGA 
GAGTATCTGG TCATTGAACG TCATGTGCCT GTTCAAACGG CCATCGAGGC CACCGAAGCA 
TCCACACAGG CTTTACTAGG TCCAGTGGTG CCGATGCAGC GACCTGGAAA TCGTTTTAGC 
TTCCCACCTG GACAAGCAAA TCAGCGCACC ATTTCGGAGT CTACAACTCA TTCAGGCGAG 
CATTACACTT CCCGCTTTCA GTCCGACTTC GAGTTGATGC AGTGCCTTGG TCGCGGTGGT 
TTCGGAGTGG TCTTCGAAGC TAAGAACAAG CTGGATGAGA ACCGATACGC CATTAAGCGC 
ATAACGCTAC CCAATAAGGA ATCTTCAAGA CAGCGAGTCC TTCGCGAGGC TCGAACTTTG 
GCTAGTTGTG AGCACCACAA CATCGTGCGA TACTTCCATT CCTGGACCGA GACACCGCCG 
ACTGGTTGGC AGGAAGAAGA GGATCGCAAG CTGCTTGCCC ACGAGCTGTC CACCTCTATC 
CAGATAGAGA CGCCGGACGA TAGTACAATG CCTTCGTTGA CGGAGCAGTT GAAGGAGAAA 
CGCCAACAGC AGCTCTTGTC CTGGGTTTCG GATGCAGCCA ATAGCACCGC TTGTAGTCAC 
GACTTTCATC TACCAGGCGA GTCATCACTC AAGAACATAC GCGAGGAGTA CGACTACGAT 
GAGGAAGAGG ACTCGCTCAT TGAATTCCGC AGCGAGTCGC AGTCAGCCGC TTTACGGGCA 
GAGGAGGAGG ATGATACCGA CGACGACTAT GAAGAGGATG AAGAGCAGCA GGGAGACCAT 
GAGAAACGAC ATCGCAGCTC CGTGTCCATT GACATCCATT CGGCCTCGTT TGACCTTAAG 
AACATAAACT ACTCACAGCA CCAACTGGTC TCCAATTCGT TTCAAATCGA ATCTGTGCGC 
CCCAAGAGCA GTGGAAGTGA TGATGCGAAT GACGATAATA AAGCTAGAAG GAAGCCCCTT 
ACCTTGGCAC TGGCCCAGAA TCACAATAAC AACCAAAACG GGAGCCAACC TACGCCCTCC 
AGTGCCACGA TTCTGAACGG AACGGTGGCC AAGCCCAGCA AGGTGTACCT CTACATCCAG 
ATGCAGCTAT GCCGCAAGGA GAGTCTGCGC GACTGGTTAA GGGACAACCG GTCGGAAACA 
AGGGCTGCCC ACATTGGTGA CATTTTTCAC CAGATCGTTG ATGCCGTTGA CTATGTACAT 
TTGAAGGGTC TGATTCATCG GGACCTGAAA CCAAGCAACA TATTCTTCTC GCAGGATGGA 
CAGATAAAGA TTGGAGACTT CGGGCTCGTC ACCGACATGG CCGATATCCC CAACCTGGTG 
GCCAAGTGTG GGGACCAGAG TGGCCTGCCC TCCTGTGCCC GCCACACCCA GCAGGTGGGC 
ACCCACTTAT ACATGTCGCC GGAGCAGCTT TTGGGCCAGC ACTACGACTA CAAGGTGGAC 
ATCTATTCAC TGGGACTGAT CTTCTTCGAG CTGCACGTGT ACTTCAGCAC TGAAATGGAG 
CGGATCAAGA CACTGCGCAG TCTTCGGGAC GGCCAGTATC CCAAGGATTT CGCTGTCAAT 
TACCCGCAGC AGTACGACTT GCTCCAGCAA ATGCTATCCG CCCAGCCAGA GCAACGCCCG 
CAGACCAAGC AGTTGAAAAG CCAGCTGCGC AACATTCTGC AGCTACCTCA TCTCCTCTCG 
GAAGGACAAA GCGAGCAGGC GGAGCTGGCG GAGCGGGCCA GGCGCCTGAG TCGCAGCCGC 
ACCTTCAGCA GCTCCAGTGA GCCGCATCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262282.1
CDS	104..3436
Translation	MFAPWPTMKTRNQWTRLSSMRLLYISTLDGRLSALDIAKSGKLRWSVPTGPGPLISSSIHRLELTNNGQFVRMIPSLSGGIYKFDGDSIDPIPITAEHLLSSSAKFSDDLVISGGKETRSYGVSVRTGQLLYECSLNGCVNSTEEGLAIDDTIREPDEEDQLEDGEQLRDEAGYIVRHDPLLDDVIIVRRQTQTVRAVESRTGVERWNFSVGQHELDLVRPSECQLQPRDELELAVLDVDIKVVVPEGIICAFSKSEPQTMLWKYKFDHPIVSAWNTNADDELQPIDLFSSAQWLWDQDENDTELPNAPQSPPSIYLGMYDKQLYIQESIRLRQEIMDQTKVYQQLTGDTSLMPRIPWKPISASSKSLVIFRKDQEDPEMIAEGAVAQGGELVPYDDENFAVAAQSVLNASEFVNGNGFYFYTTGDLNGPQECSTQNNPTDLPAITAPTSPTNATSEGTEATGNHSVNDDLGFSLDDIDAPVKVVILSLWFWWKEIVVIAFTSAVILNIFMGQRNQRVEREYLVIERHVPVQTAIEATEASTQALLGPVVPMQRPGNRFSFPPGQANQRTISESTTHSGEHYTSRFQSDFELMQCLGRGGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRVLREARTLASCEHHNIVRYFHSWTETPPTGWQEEEDRKLLAHELSTSIQIETPDDSTMPSLTEQLKEKRQQQLLSWVSDAANSTACSHDFHLPGESSLKNIREEYDYDEEEDSLIEFRSESQSAALRAEEEDDTDDDYEEDEEQQGDHEKRHRSSVSIDIHSASFDLKNINYSQHQLVSNSFQIESVRPKSSGSDDANDDNKARRKPLTLALAQNHNNNQNGSQPTPSSATILNGTVAKPSKVYLYIQMQLCRKESLRDWLRDNRSETRAAHIGDIFHQIVDAVDYVHLKGLIHRDLKPSNIFFSQDGQIKIGDFGLVTDMADIPNLVAKCGDQSGLPSCARHTQQVGTHLYMSPEQLLGQHYDYKVDIYSLGLIFFELHVYFSTEMERIKTLRSLRDGQYPKDFAVNYPQQYDLLQQMLSAQPEQRPQTKQLKSQLRNILQLPHLLSEGQSEQAELAERARRLSRSRTFSSSSEPHQ

Target ORF information:

RefSeq Version	NM_001275353.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pancreatic eIF-2alpha kinase (PEK), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275353.1

ORF Insert Sequence:

ATGTTTGCAC CTTGGCCAAC TATGAAAACC CGTAATCAGT GGACGCGCTT ATCAAGCATG 
CGACTCCTAT ACATTTCCAC GCTTGATGGT CGACTGAGCG CTCTGGACAT CGCCAAGTCC 
GGCAAACTGC GCTGGAGTGT GCCCACCGGA CCGGGGCCAC TAATCTCGTC CAGCATCCAT 
CGATTGGAGC TGACCAACAA TGGGCAGTTC GTACGCATGA TTCCCTCTTT GAGCGGCGGC 
ATCTACAAGT TCGACGGGGA CTCCATCGAT CCCATTCCAA TCACGGCGGA GCACCTACTC 
AGCTCGAGCG CCAAATTCAG CGATGATCTG GTTATATCCG GAGGCAAGGA GACACGCTCA 
TACGGTGTTT CCGTTCGCAC TGGCCAGCTG CTGTATGAAT GTTCTCTAAA CGGATGTGTA 
AACTCTACGG AGGAAGGGCT TGCAATCGAC GACACCATTA GGGAGCCCGA TGAGGAGGAT 
CAGCTGGAGG ACGGAGAACA GTTAAGGGAC GAGGCCGGCT ACATAGTGCG CCATGATCCT 
CTCCTTGACG ACGTTATTAT CGTCCGCCGC CAGACACAAA CGGTTCGGGC CGTGGAATCC 
CGGACCGGAG TGGAGCGTTG GAACTTTAGC GTAGGTCAGC ATGAGTTGGA TCTGGTGAGA 
CCTTCCGAGT GCCAGCTACA GCCGCGCGAT GAGCTGGAGC TGGCTGTTTT GGATGTGGAT 
ATAAAGGTGG TAGTGCCCGA GGGAATTATC TGCGCCTTCA GCAAAAGTGA GCCACAGACT 
ATGCTGTGGA AGTACAAGTT TGATCACCCA ATTGTGAGTG CCTGGAATAC AAATGCCGAC 
GACGAATTGC AGCCTATTGA TTTGTTTAGC TCGGCCCAGT GGCTTTGGGA CCAGGACGAA 
AATGACACGG AGCTGCCCAA TGCGCCACAA AGCCCGCCAT CCATCTACTT GGGCATGTAC 
GATAAACAGT TGTATATTCA GGAGTCAATA CGTCTACGCC AGGAGATTAT GGACCAGACC 
AAGGTCTATC AGCAGCTTAC AGGCGACACC AGCCTGATGC CCAGGATTCC CTGGAAGCCG 
ATATCCGCCA GTAGCAAATC GCTGGTAATC TTCCGGAAAG ACCAGGAGGA CCCTGAAATG 
ATTGCTGAGG GAGCTGTGGC ACAAGGAGGG GAACTGGTGC CCTATGATGA TGAAAACTTC 
GCTGTGGCAG CGCAGTCCGT ATTGAATGCA TCGGAGTTTG TCAATGGCAA TGGTTTTTAC 
TTCTACACCA CTGGCGATCT GAACGGCCCG CAAGAGTGCA GTACGCAGAA TAATCCAACT 
GACCTTCCAG CAATCACAGC TCCAACATCG CCTACCAATG CCACATCTGA GGGAACGGAA 
GCTACTGGAA ACCATAGCGT CAACGACGAT TTGGGCTTTA GTTTGGATGA CATTGATGCG 
CCTGTAAAGG TTGTGATATT GTCGTTGTGG TTCTGGTGGA AGGAAATCGT GGTCATAGCA 
TTTACGTCGG CTGTGATTCT AAATATTTTC ATGGGCCAAC GAAACCAGCG CGTGGAGCGA 
GAGTATCTGG TCATTGAACG TCATGTGCCT GTTCAAACGG CCATCGAGGC CACCGAAGCA 
TCCACACAGG CTTTACTAGG TCCAGTGGTG CCGATGCAGC GACCTGGAAA TCGTTTTAGC 
TTCCCACCTG GACAAGCAAA TCAGCGCACC ATTTCGGAGT CTACAACTCA TTCAGGCGAG 
CATTACACTT CCCGCTTTCA GTCCGACTTC GAGTTGATGC AGTGCCTTGG TCGCGGTGGT 
TTCGGAGTGG TCTTCGAAGC TAAGAACAAG CTGGATGAGA ACCGATACGC CATTAAGCGC 
ATAACGCTAC CCAATAAGGA ATCTTCAAGA CAGCGAGTCC TTCGCGAGGC TCGAACTTTG 
GCTAGTTGTG AGCACCACAA CATCGTGCGA TACTTCCATT CCTGGACCGA GACACCGCCG 
ACTGGTTGGC AGGAAGAAGA GGATCGCAAG CTGCTTGCCC ACGAGCTGTC CACCTCTATC 
CAGATAGAGA CGCCGGACGA TAGTACAATG CCTTCGTTGA CGGAGCAGTT GAAGGAGAAA 
CGCCAACAGC AGCTCTTGTC CTGGGTTTCG GATGCAGCCA ATAGCACCGC TTGTAGTCAC 
GACTTTCATC TACCAGGCGA GTCATCACTC AAGAACATAC GCGAGGAGTA CGACTACGAT 
GAGGAAGAGG ACTCGCTCAT TGAATTCCGC AGCGAGTCGC AGTCAGCCGC TTTACGGGCA 
GAGGAGGAGG ATGATACCGA CGACGACTAT GAAGAGGATG AAGAGCAGCA GGGAGACCAT 
GAGAAACGAC ATCGCAGCTC CGTGTCCATT GACATCCATT CGGCCTCGTT TGACCTTAAG 
AACATAAACT ACTCACAGCA CCAACTGGTC TCCAATTCGT TTCAAATCGA ATCTGTGCGC 
CCCAAGAGCA GTGGAAGTGA TGATGCGAAT GACGATAATA AAGCTAGAAG GAAGCCCCTT 
ACCTTGGCAC TGGCCCAGAA TCACAATAAC AACCAAAACG GGAGCCAACC TACGCCCTCC 
AGTGCCACGA TTCTGAACGG AACGGTGGCC AAGCCCAGCA AGGTGTACCT CTACATCCAG 
ATGCAGCTAT GCCGCAAGGA GAGTCTGCGC GACTGGTTAA GGGACAACCG GTCGGAAACA 
AGGGCTGCCC ACATTGGTGA CATTTTTCAC CAGATCGTTG ATGCCGTTGA CTATGTACAT 
TTGAAGGGTC TGATTCATCG GGACCTGAAA CCAAGCAACA TATTCTTCTC GCAGGATGGA 
CAGATAAAGA TTGGAGACTT CGGGCTCGTC ACCGACATGG CCGATATCCC CAACCTGGTG 
GCCAAGTGTG GGGACCAGAG TGGCCTGCCC TCCTGTGCCC GCCACACCCA GCAGGTGGGC 
ACCCACTTAT ACATGTCGCC GGAGCAGCTT TTGGGCCAGC ACTACGACTA CAAGGTGGAC 
ATCTATTCAC TGGGACTGAT CTTCTTCGAG CTGCACGTGT ACTTCAGCAC TGAAATGGAG 
CGGATCAAGA CACTGCGCAG TCTTCGGGAC GGCCAGTATC CCAAGGATTT CGCTGTCAAT 
TACCCGCAGC AGTACGACTT GCTCCAGCAA ATGCTATCCG CCCAGCCAGA GCAACGCCCG 
CAGACCAAGC AGTTGAAAAG CCAGCTGCGC AACATTCTGC AGCTACCTCA TCTCCTCTCG 
GAAGGACAAA GCGAGCAGGC GGAGCTGGCG GAGCGGGCCA GGCGCCTGAG TCGCAGCCGC 
ACCTTCAGCA GCTCCAGTGA GCCGCATCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEK ( NM_001275354.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262283.1 Drosophila melanogaster pancreatic eIF-2alpha kinase (PEK), transcript variant C, mRNA.

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CloneID	OFa07710
Clone ID Related Accession (Same CDS sequence)	NM_001275354.1
Accession Version	NM_001275354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pancreatic eIF-2alpha kinase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTAATTG GATATTACCT ACATAAAGGA CTCCTATACA TTTCCACGCT TGATGGTCGA 
CTGAGCGCTC TGGACATCGC CAAGTCCGGC AAACTGCGCT GGAGTGTGCC CACCGGACCG 
GGGCCACTAA TCTCGTCCAG CATCCATCGA TTGGAGCTGA CCAACAATGG GCAGTTCGTA 
CGCATGATTC CCTCTTTGAG CGGCGGCATC TACAAGTTCG ACGGGGACTC CATCGATCCC 
ATTCCAATCA CGGCGGAGCA CCTACTCAGC TCGAGCGCCA AATTCAGCGA TGATCTGGTT 
ATATCCGGAG GCAAGGAGAC ACGCTCATAC GGTGTTTCCG TTCGCACTGG CCAGCTGCTG 
TATGAATGTT CTCTAAACGG ATGTGTAAAC TCTACGGAGG AAGGGCTTGC AATCGACGAC 
ACCATTAGGG AGCCCGATGA GGAGGATCAG CTGGAGGACG GAGAACAGTT AAGGGACGAG 
GCCGGCTACA TAGTGCGCCA TGATCCTCTC CTTGACGACG TTATTATCGT CCGCCGCCAG 
ACACAAACGG TTCGGGCCGT GGAATCCCGG ACCGGAGTGG AGCGTTGGAA CTTTAGCGTA 
GGTCAGCATG AGTTGGATCT GGTGAGACCT TCCGAGTGCC AGCTACAGCC GCGCGATGAG 
CTGGAGCTGG CTGTTTTGGA TGTGGATATA AAGGTGGTAG TGCCCGAGGG AATTATCTGC 
GCCTTCAGCA AAAGTGAGCC ACAGACTATG CTGTGGAAGT ACAAGTTTGA TCACCCAATT 
GTGAGTGCCT GGAATACAAA TGCCGACGAC GAATTGCAGC CTATTGATTT GTTTAGCTCG 
GCCCAGTGGC TTTGGGACCA GGACGAAAAT GACACGGAGC TGCCCAATGC GCCACAAAGC 
CCGCCATCCA TCTACTTGGG CATGTACGAT AAACAGTTGT ATATTCAGGA GTCAATACGT 
CTACGCCAGG AGATTATGGA CCAGACCAAG GTCTATCAGC AGCTTACAGG CGACACCAGC 
CTGATGCCCA GGATTCCCTG GAAGCCGATA TCCGCCAGTA GCAAATCGCT GGTAATCTTC 
CGGAAAGACC AGGAGGACCC TGAAATGATT GCTGAGGGAG CTGTGGCACA AGGAGGGGAA 
CTGGTGCCCT ATGATGATGA AAACTTCGCT GTGGCAGCGC AGTCCGTATT GAATGCATCG 
GAGTTTGTCA ATGGCAATGG TTTTTACTTC TACACCACTG GCGATCTGAA CGGCCCGCAA 
GAGTGCAGTA CGCAGAATAA TCCAACTGAC CTTCCAGCAA TCACAGCTCC AACATCGCCT 
ACCAATGCCA CATCTGAGGG AACGGAAGCT ACTGGAAACC ATAGCGTCAA CGACGATTTG 
GGCTTTAGTT TGGATGACAT TGATGCGCCT GTAAAGGTTG TGATATTGTC GTTGTGGTTC 
TGGTGGAAGG AAATCGTGGT CATAGCATTT ACGTCGGCTG TGATTCTAAA TATTTTCATG 
GGCCAACGAA ACCAGCGCGT GGAGCGAGAG TATCTGGTCA TTGAACGTCA TGTGCCTGTT 
CAAACGGCCA TCGAGGCCAC CGAAGCATCC ACACAGGCTT TACTAGGTCC AGTGGTGCCG 
ATGCAGCGAC CTGGAAATCG TTTTAGCTTC CCACCTGGAC AAGCAAATCA GCGCACCATT 
TCGGAGTCTA CAACTCATTC AGGCGAGCAT TACACTTCCC GCTTTCAGTC CGACTTCGAG 
TTGATGCAGT GCCTTGGTCG CGGTGGTTTC GGAGTGGTCT TCGAAGCTAA GAACAAGCTG 
GATGAGAACC GATACGCCAT TAAGCGCATA ACGCTACCCA ATAAGGAATC TTCAAGACAG 
CGAGTCCTTC GCGAGGCTCG AACTTTGGCT AGTTGTGAGC ACCACAACAT CGTGCGATAC 
TTCCATTCCT GGACCGAGAC ACCGCCGACT GGTTGGCAGG AAGAAGAGGA TCGCAAGCTG 
CTTGCCCACG AGCTGTCCAC CTCTATCCAG ATAGAGACGC CGGACGATAG TACAATGCCT 
TCGTTGACGG AGCAGTTGAA GGAGAAACGC CAACAGCAGC TCTTGTCCTG GGTTTCGGAT 
GCAGCCAATA GCACCGCTTG TAGTCACGAC TTTCATCTAC CAGGCGAGTC ATCACTCAAG 
AACATACGCG AGGAGTACGA CTACGATGAG GAAGAGGACT CGCTCATTGA ATTCCGCAGC 
GAGTCGCAGT CAGCCGCTTT ACGGGCAGAG GAGGAGGATG ATACCGACGA CGACTATGAA 
GAGGATGAAG AGCAGCAGGG AGACCATGAG AAACGACATC GCAGCTCCGT GTCCATTGAC 
ATCCATTCGG CCTCGTTTGA CCTTAAGAAC ATAAACTACT CACAGCACCA ACTGGTCTCC 
AATTCGTTTC AAATCGAATC TGTGCGCCCC AAGAGCAGTG GAAGTGATGA TGCGAATGAC 
GATAATAAAG CTAGAAGGAA GCCCCTTACC TTGGCACTGG CCCAGAATCA CAATAACAAC 
CAAAACGGGA GCCAACCTAC GCCCTCCAGT GCCACGATTC TGAACGGAAC GGTGGCCAAG 
CCCAGCAAGG TGTACCTCTA CATCCAGATG CAGCTATGCC GCAAGGAGAG TCTGCGCGAC 
TGGTTAAGGG ACAACCGGTC GGAAACAAGG GCTGCCCACA TTGGTGACAT TTTTCACCAG 
ATCGTTGATG CCGTTGACTA TGTACATTTG AAGGGTCTGA TTCATCGGGA CCTGAAACCA 
AGCAACATAT TCTTCTCGCA GGATGGACAG ATAAAGATTG GAGACTTCGG GCTCGTCACC 
GACATGGCCG ATATCCCCAA CCTGGTGGCC AAGTGTGGGG ACCAGAGTGG CCTGCCCTCC 
TGTGCCCGCC ACACCCAGCA GGTGGGCACC CACTTATACA TGTCGCCGGA GCAGCTTTTG 
GGCCAGCACT ACGACTACAA GGTGGACATC TATTCACTGG GACTGATCTT CTTCGAGCTG 
CACGTGTACT TCAGCACTGA AATGGAGCGG ATCAAGACAC TGCGCAGTCT TCGGGACGGC 
CAGTATCCCA AGGATTTCGC TGTCAATTAC CCGCAGCAGT ACGACTTGCT CCAGCAAATG 
CTATCCGCCC AGCCAGAGCA ACGCCCGCAG ACCAAGCAGT TGAAAAGCCA GCTGCGCAAC 
ATTCTGCAGC TACCTCATCT CCTCTCGGAA GGACAAAGCG AGCAGGCGGA GCTGGCGGAG 
CGGGCCAGGC GCCTGAGTCG CAGCCGCACC TTCAGCAGCT CCAGTGAGCC GCATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262283.1
CDS	41..3340
Translation	MVIGYYLHKGLLYISTLDGRLSALDIAKSGKLRWSVPTGPGPLISSSIHRLELTNNGQFVRMIPSLSGGIYKFDGDSIDPIPITAEHLLSSSAKFSDDLVISGGKETRSYGVSVRTGQLLYECSLNGCVNSTEEGLAIDDTIREPDEEDQLEDGEQLRDEAGYIVRHDPLLDDVIIVRRQTQTVRAVESRTGVERWNFSVGQHELDLVRPSECQLQPRDELELAVLDVDIKVVVPEGIICAFSKSEPQTMLWKYKFDHPIVSAWNTNADDELQPIDLFSSAQWLWDQDENDTELPNAPQSPPSIYLGMYDKQLYIQESIRLRQEIMDQTKVYQQLTGDTSLMPRIPWKPISASSKSLVIFRKDQEDPEMIAEGAVAQGGELVPYDDENFAVAAQSVLNASEFVNGNGFYFYTTGDLNGPQECSTQNNPTDLPAITAPTSPTNATSEGTEATGNHSVNDDLGFSLDDIDAPVKVVILSLWFWWKEIVVIAFTSAVILNIFMGQRNQRVEREYLVIERHVPVQTAIEATEASTQALLGPVVPMQRPGNRFSFPPGQANQRTISESTTHSGEHYTSRFQSDFELMQCLGRGGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRVLREARTLASCEHHNIVRYFHSWTETPPTGWQEEEDRKLLAHELSTSIQIETPDDSTMPSLTEQLKEKRQQQLLSWVSDAANSTACSHDFHLPGESSLKNIREEYDYDEEEDSLIEFRSESQSAALRAEEEDDTDDDYEEDEEQQGDHEKRHRSSVSIDIHSASFDLKNINYSQHQLVSNSFQIESVRPKSSGSDDANDDNKARRKPLTLALAQNHNNNQNGSQPTPSSATILNGTVAKPSKVYLYIQMQLCRKESLRDWLRDNRSETRAAHIGDIFHQIVDAVDYVHLKGLIHRDLKPSNIFFSQDGQIKIGDFGLVTDMADIPNLVAKCGDQSGLPSCARHTQQVGTHLYMSPEQLLGQHYDYKVDIYSLGLIFFELHVYFSTEMERIKTLRSLRDGQYPKDFAVNYPQQYDLLQQMLSAQPEQRPQTKQLKSQLRNILQLPHLLSEGQSEQAELAERARRLSRSRTFSSSSEPHQ

Target ORF information:

RefSeq Version	NM_001275354.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pancreatic eIF-2alpha kinase (PEK), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275354.1

ORF Insert Sequence:

ATGGTAATTG GATATTACCT ACATAAAGGA CTCCTATACA TTTCCACGCT TGATGGTCGA 
CTGAGCGCTC TGGACATCGC CAAGTCCGGC AAACTGCGCT GGAGTGTGCC CACCGGACCG 
GGGCCACTAA TCTCGTCCAG CATCCATCGA TTGGAGCTGA CCAACAATGG GCAGTTCGTA 
CGCATGATTC CCTCTTTGAG CGGCGGCATC TACAAGTTCG ACGGGGACTC CATCGATCCC 
ATTCCAATCA CGGCGGAGCA CCTACTCAGC TCGAGCGCCA AATTCAGCGA TGATCTGGTT 
ATATCCGGAG GCAAGGAGAC ACGCTCATAC GGTGTTTCCG TTCGCACTGG CCAGCTGCTG 
TATGAATGTT CTCTAAACGG ATGTGTAAAC TCTACGGAGG AAGGGCTTGC AATCGACGAC 
ACCATTAGGG AGCCCGATGA GGAGGATCAG CTGGAGGACG GAGAACAGTT AAGGGACGAG 
GCCGGCTACA TAGTGCGCCA TGATCCTCTC CTTGACGACG TTATTATCGT CCGCCGCCAG 
ACACAAACGG TTCGGGCCGT GGAATCCCGG ACCGGAGTGG AGCGTTGGAA CTTTAGCGTA 
GGTCAGCATG AGTTGGATCT GGTGAGACCT TCCGAGTGCC AGCTACAGCC GCGCGATGAG 
CTGGAGCTGG CTGTTTTGGA TGTGGATATA AAGGTGGTAG TGCCCGAGGG AATTATCTGC 
GCCTTCAGCA AAAGTGAGCC ACAGACTATG CTGTGGAAGT ACAAGTTTGA TCACCCAATT 
GTGAGTGCCT GGAATACAAA TGCCGACGAC GAATTGCAGC CTATTGATTT GTTTAGCTCG 
GCCCAGTGGC TTTGGGACCA GGACGAAAAT GACACGGAGC TGCCCAATGC GCCACAAAGC 
CCGCCATCCA TCTACTTGGG CATGTACGAT AAACAGTTGT ATATTCAGGA GTCAATACGT 
CTACGCCAGG AGATTATGGA CCAGACCAAG GTCTATCAGC AGCTTACAGG CGACACCAGC 
CTGATGCCCA GGATTCCCTG GAAGCCGATA TCCGCCAGTA GCAAATCGCT GGTAATCTTC 
CGGAAAGACC AGGAGGACCC TGAAATGATT GCTGAGGGAG CTGTGGCACA AGGAGGGGAA 
CTGGTGCCCT ATGATGATGA AAACTTCGCT GTGGCAGCGC AGTCCGTATT GAATGCATCG 
GAGTTTGTCA ATGGCAATGG TTTTTACTTC TACACCACTG GCGATCTGAA CGGCCCGCAA 
GAGTGCAGTA CGCAGAATAA TCCAACTGAC CTTCCAGCAA TCACAGCTCC AACATCGCCT 
ACCAATGCCA CATCTGAGGG AACGGAAGCT ACTGGAAACC ATAGCGTCAA CGACGATTTG 
GGCTTTAGTT TGGATGACAT TGATGCGCCT GTAAAGGTTG TGATATTGTC GTTGTGGTTC 
TGGTGGAAGG AAATCGTGGT CATAGCATTT ACGTCGGCTG TGATTCTAAA TATTTTCATG 
GGCCAACGAA ACCAGCGCGT GGAGCGAGAG TATCTGGTCA TTGAACGTCA TGTGCCTGTT 
CAAACGGCCA TCGAGGCCAC CGAAGCATCC ACACAGGCTT TACTAGGTCC AGTGGTGCCG 
ATGCAGCGAC CTGGAAATCG TTTTAGCTTC CCACCTGGAC AAGCAAATCA GCGCACCATT 
TCGGAGTCTA CAACTCATTC AGGCGAGCAT TACACTTCCC GCTTTCAGTC CGACTTCGAG 
TTGATGCAGT GCCTTGGTCG CGGTGGTTTC GGAGTGGTCT TCGAAGCTAA GAACAAGCTG 
GATGAGAACC GATACGCCAT TAAGCGCATA ACGCTACCCA ATAAGGAATC TTCAAGACAG 
CGAGTCCTTC GCGAGGCTCG AACTTTGGCT AGTTGTGAGC ACCACAACAT CGTGCGATAC 
TTCCATTCCT GGACCGAGAC ACCGCCGACT GGTTGGCAGG AAGAAGAGGA TCGCAAGCTG 
CTTGCCCACG AGCTGTCCAC CTCTATCCAG ATAGAGACGC CGGACGATAG TACAATGCCT 
TCGTTGACGG AGCAGTTGAA GGAGAAACGC CAACAGCAGC TCTTGTCCTG GGTTTCGGAT 
GCAGCCAATA GCACCGCTTG TAGTCACGAC TTTCATCTAC CAGGCGAGTC ATCACTCAAG 
AACATACGCG AGGAGTACGA CTACGATGAG GAAGAGGACT CGCTCATTGA ATTCCGCAGC 
GAGTCGCAGT CAGCCGCTTT ACGGGCAGAG GAGGAGGATG ATACCGACGA CGACTATGAA 
GAGGATGAAG AGCAGCAGGG AGACCATGAG AAACGACATC GCAGCTCCGT GTCCATTGAC 
ATCCATTCGG CCTCGTTTGA CCTTAAGAAC ATAAACTACT CACAGCACCA ACTGGTCTCC 
AATTCGTTTC AAATCGAATC TGTGCGCCCC AAGAGCAGTG GAAGTGATGA TGCGAATGAC 
GATAATAAAG CTAGAAGGAA GCCCCTTACC TTGGCACTGG CCCAGAATCA CAATAACAAC 
CAAAACGGGA GCCAACCTAC GCCCTCCAGT GCCACGATTC TGAACGGAAC GGTGGCCAAG 
CCCAGCAAGG TGTACCTCTA CATCCAGATG CAGCTATGCC GCAAGGAGAG TCTGCGCGAC 
TGGTTAAGGG ACAACCGGTC GGAAACAAGG GCTGCCCACA TTGGTGACAT TTTTCACCAG 
ATCGTTGATG CCGTTGACTA TGTACATTTG AAGGGTCTGA TTCATCGGGA CCTGAAACCA 
AGCAACATAT TCTTCTCGCA GGATGGACAG ATAAAGATTG GAGACTTCGG GCTCGTCACC 
GACATGGCCG ATATCCCCAA CCTGGTGGCC AAGTGTGGGG ACCAGAGTGG CCTGCCCTCC 
TGTGCCCGCC ACACCCAGCA GGTGGGCACC CACTTATACA TGTCGCCGGA GCAGCTTTTG 
GGCCAGCACT ACGACTACAA GGTGGACATC TATTCACTGG GACTGATCTT CTTCGAGCTG 
CACGTGTACT TCAGCACTGA AATGGAGCGG ATCAAGACAC TGCGCAGTCT TCGGGACGGC 
CAGTATCCCA AGGATTTCGC TGTCAATTAC CCGCAGCAGT ACGACTTGCT CCAGCAAATG 
CTATCCGCCC AGCCAGAGCA ACGCCCGCAG ACCAAGCAGT TGAAAAGCCA GCTGCGCAAC 
ATTCTGCAGC TACCTCATCT CCTCTCGGAA GGACAAAGCG AGCAGGCGGA GCTGGCGGAG 
CGGGCCAGGC GCCTGAGTCG CAGCCGCACC TTCAGCAGCT CCAGTGAGCC GCATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PEK cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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