Cerk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cerk
Entrez Gene ID	40640
Full Name	Ceramide kinase
Synonyms	CG16708,CerK,DCERK,Dcerk,Dmel\CG16708,dcerk
General protein information	Preferred Names Ceramide kinase Names CG16708 gene product from transcript CG16708-RA CG16708-PA CG16708-PB Cerk-PA Cerk-PB ceramide kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 82F11-83A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_169050.2	NP_730923.1	ceramide kinase, isoform A
NM_141273.3	NP_649530.2	ceramide kinase, isoform B

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	T10B11.2	NP_491977.1
Homo sapiens (human)	CERK	NP_073603.2
Danio rerio (zebrafish)	cerk	NP_001099056.1
Drosophila melanogaster (fruit fly)	Cerk	NP_730923.1
Gallus gallus (chicken)	CERK	NP_001026511.1
Pan troglodytes (chimpanzee)	CERK	XP_001137835.1
	CERK	XP_001111087.1
Rattus norvegicus (Norway rat)	Cerk	NP_001128333.1
Xenopus tropicalis (tropical clawed frog)	LOC100487080	XP_004913055.1
Canis lupus familiaris (dog)	CERK	XP_005626265.1
Mus musculus (house mouse)	Cerk	NP_663450.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002932	XP_563184.4
Arabidopsis thaliana (thale cress)	AT5G51290	NP_568756.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cerk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cerk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03914	NM_169050.2 Latest version!	Drosophila melanogaster ceramide kinase, transcript variant A (Cerk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa03914	NM_141273.3 Latest version!	Drosophila melanogaster ceramide kinase, transcript variant B (Cerk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cerk ( NM_169050.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730923.1 Drosophila melanogaster ceramide kinase, transcript variant A (Cerk), mRNA.

Price & Availability↑

CloneID	OFa03914
Clone ID Related Accession (Same CDS sequence)	NM_169050.2 , NM_141273.3
Accession Version	NM_169050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ceramide kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169050.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAGA CCAAGCAGCC AGCAGGGCGG TCGTCCACCT CGGCCGGGGA TTTTGTAGCC 
CCCATTAGCT CTACGAGTGC CTCCAAGTTC ACGCCCTGCT CCGCCTCCGA CCACAATGTC 
CTGCTGAACA ACTTCCAGCT TAAGAAGAAG AGCTACAGGG TCCTGCTTCA CGGACAGCAG 
TTGGTTTGGG AGCGTCTGCA GAAGATCAAG CAATCGCCCC AAGGAAACGA GGCCAAGGCA 
CCGTTGCCGC CGGACTCGCC AGCTCAACCA GGCGGAATCT GCTCTTATGG ACCACAAAGC 
CACGTGCTCC ACCTGGACGA TGTGGTTAGC ATAAGGAGCG GGGACACCAA GGCGAGCTCC 
CTCAAACCGC CTTCGCCGCC AGGCTCGGAA CGCAGTTCCG GATGCAGTGG CGATGTTGCT 
CAAGGGAAGC CTACCAGTCA ATACCTGACC ATCAACTATG CCATGAGGCT GAGCAAGTCC 
CAAACGGATT GCAATCGGTG GGAGCTAAGG AGACTCACCT TCTTCAACTC GGATCCCTAC 
ATAGTGAGGC AGTGGGACCA GGAGCTGCAA ATCCGTCTGC ATAGCTCCTC CCCCACCCGG 
ATGCGAGTGC GCCGCCTTTT GGTCTTTATA AACCCCTATG GAGGTCGCAA GGCGGGAGCT 
CAGACCTATG AGCGCCATGT GAGACCTATT TTCCAGCTTG CCGGCGTAGA CGCCACGTGT 
ATCACCACTC AGAGGGCAAA CCAAGTGAAG GACATACTTC TGAGCCATGA TCTGGGAGTA 
TACGATGCGG TTTGCTGTGT CGGAGGCGAT GGCACCGTAG CAGAGGTCAT CAACGGACTG 
ATATTCCGTC AAATGCGAGA GTTGGGACTG GACGAACAGC GGCCACCATA CATTCCAAGA 
CCGGCTCTGC CAGTGGGTGT GATTCCCGCT GGCAGCACCG ACACCATTGC GTATAGTATG 
CACGGCACGG CGGATGTGAG GACAGCGGCT ATCCATGTGA TTCTGGGCCA GCATCGGGGA 
TTGGATGTGT GCAGTGTGAG CAATGGGCAG TCCCTGCTCA GATTCTGTGC CAGTGTCCTG 
AGCTACGGGT ACCTGGGCGA TGTGGCAGCC CAGAGCGAGA ACTACCGCTG GATGGGACCG 
CGCCGGTACG AGTACAGTGG CGTCAAGGCC TTCCTGAATA ATCGCGGCTA TGACGCCGAA 
CTGAGAATGT TAGAAGAGCC CGATCTTCTA CTGACCACGC CGCTGGAGGA CATTCCGCAG 
AGTCCGGATA GTGTGTGCTC GCTGGGAGAG TCAGTGCCAT CCGTCTGCTA TGCCAATTGC 
CAGCGCTGCA GCTTCGCCAG CAGCATACAG GAACAGCGAT CCTCATTGTT CATCCAAGAG 
GAATCTAAAG AGGCAGAGCG CAATCAGCAG GTAGAAACAG AGGACTCTCA TCTAGCCGCC 
AGTGAAGCAG CACTCCTGAG GCCTCGTCCG CGTCCAGGCA ATCTTCGATT GCCCACTGGC 
TCCATTTCAT CAATGAGGAA CCTCGGCAAC GATCAGTGGA AGGTTGTGCG GGGCAATTTC 
TTTATGATCT GCGGCGCGAA CATAACCTGC GCCTGCGCCA GGAGTCCCAA TGGCATCTCC 
CGTTACAGTC ATCTGGGTGA TGGTTGCCTG GACCTGATTC TCGTGAAAAA GACCTCACTG 
CTCAACAACG TGCGTTTTCT GCTCAACACG GCGGGCAGAA GTGGTGATAT TCGCAATTTG 
CCTTTTGTAG AGGTATATCG CACAAGGGAA TTCCGGTTCA GAACTTTTTC TGCCAGCGAG 
GAGGACTACA GCTTGGCAGG GTCTTGTCAG CCGATTACAC CGCCTGAAGA AATGACCGCC 
CACTCCTCCT CGACGGAGTT TTCCAGCTGG AACTGTGATG GAGAGGTGGT GACCGACCTG 
GACATAACCA TGCGATCGCA TTGTCAGCTC ATCGAGGTCT TCATGCGGGG TCCCCATTCC 
TACAGCAAGC CCCTCAAGGG CGGCACCACG CCCACAACCC CAGCTAGCTC TAGCGACAAT 
GTTTACTGCT GCTGCAAGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730923.1
CDS	438..2501
Translation	MTQTKQPAGRSSTSAGDFVAPISSTSASKFTPCSASDHNVLLNNFQLKKKSYRVLLHGQQLVWERLQKIKQSPQGNEAKAPLPPDSPAQPGGICSYGPQSHVLHLDDVVSIRSGDTKASSLKPPSPPGSERSSGCSGDVAQGKPTSQYLTINYAMRLSKSQTDCNRWELRRLTFFNSDPYIVRQWDQELQIRLHSSSPTRMRVRRLLVFINPYGGRKAGAQTYERHVRPIFQLAGVDATCITTQRANQVKDILLSHDLGVYDAVCCVGGDGTVAEVINGLIFRQMRELGLDEQRPPYIPRPALPVGVIPAGSTDTIAYSMHGTADVRTAAIHVILGQHRGLDVCSVSNGQSLLRFCASVLSYGYLGDVAAQSENYRWMGPRRYEYSGVKAFLNNRGYDAELRMLEEPDLLLTTPLEDIPQSPDSVCSLGESVPSVCYANCQRCSFASSIQEQRSSLFIQEESKEAERNQQVETEDSHLAASEAALLRPRPRPGNLRLPTGSISSMRNLGNDQWKVVRGNFFMICGANITCACARSPNGISRYSHLGDGCLDLILVKKTSLLNNVRFLLNTAGRSGDIRNLPFVEVYRTREFRFRTFSASEEDYSLAGSCQPITPPEEMTAHSSSTEFSSWNCDGEVVTDLDITMRSHCQLIEVFMRGPHSYSKPLKGGTTPTTPASSSDNVYCCCKD

Target ORF information:

RefSeq Version	NM_169050.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ceramide kinase, transcript variant A (Cerk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169050.2

ORF Insert Sequence:

ATGACGCAGA CCAAGCAGCC AGCAGGGCGG TCGTCCACCT CGGCCGGGGA TTTTGTAGCC 
CCCATTAGCT CTACGAGTGC CTCCAAGTTC ACGCCCTGCT CCGCCTCCGA CCACAATGTC 
CTGCTGAACA ACTTCCAGCT TAAGAAGAAG AGCTACAGGG TCCTGCTTCA CGGACAGCAG 
TTGGTTTGGG AGCGTCTGCA GAAGATCAAG CAATCGCCCC AAGGAAACGA GGCCAAGGCA 
CCGTTGCCGC CGGACTCGCC AGCTCAACCA GGCGGAATCT GCTCTTATGG ACCACAAAGC 
CACGTGCTCC ACCTGGACGA TGTGGTTAGC ATAAGGAGCG GGGACACCAA GGCGAGCTCC 
CTCAAACCGC CTTCGCCGCC AGGCTCGGAA CGCAGTTCCG GATGCAGTGG CGATGTTGCT 
CAAGGGAAGC CTACCAGTCA ATACCTGACC ATCAACTATG CCATGAGGCT GAGCAAGTCC 
CAAACGGATT GCAATCGGTG GGAGCTAAGG AGACTCACCT TCTTCAACTC GGATCCCTAC 
ATAGTGAGGC AGTGGGACCA GGAGCTGCAA ATCCGTCTGC ATAGCTCCTC CCCCACCCGG 
ATGCGAGTGC GCCGCCTTTT GGTCTTTATA AACCCCTATG GAGGTCGCAA GGCGGGAGCT 
CAGACCTATG AGCGCCATGT GAGACCTATT TTCCAGCTTG CCGGCGTAGA CGCCACGTGT 
ATCACCACTC AGAGGGCAAA CCAAGTGAAG GACATACTTC TGAGCCATGA TCTGGGAGTA 
TACGATGCGG TTTGCTGTGT CGGAGGCGAT GGCACCGTAG CAGAGGTCAT CAACGGACTG 
ATATTCCGTC AAATGCGAGA GTTGGGACTG GACGAACAGC GGCCACCATA CATTCCAAGA 
CCGGCTCTGC CAGTGGGTGT GATTCCCGCT GGCAGCACCG ACACCATTGC GTATAGTATG 
CACGGCACGG CGGATGTGAG GACAGCGGCT ATCCATGTGA TTCTGGGCCA GCATCGGGGA 
TTGGATGTGT GCAGTGTGAG CAATGGGCAG TCCCTGCTCA GATTCTGTGC CAGTGTCCTG 
AGCTACGGGT ACCTGGGCGA TGTGGCAGCC CAGAGCGAGA ACTACCGCTG GATGGGACCG 
CGCCGGTACG AGTACAGTGG CGTCAAGGCC TTCCTGAATA ATCGCGGCTA TGACGCCGAA 
CTGAGAATGT TAGAAGAGCC CGATCTTCTA CTGACCACGC CGCTGGAGGA CATTCCGCAG 
AGTCCGGATA GTGTGTGCTC GCTGGGAGAG TCAGTGCCAT CCGTCTGCTA TGCCAATTGC 
CAGCGCTGCA GCTTCGCCAG CAGCATACAG GAACAGCGAT CCTCATTGTT CATCCAAGAG 
GAATCTAAAG AGGCAGAGCG CAATCAGCAG GTAGAAACAG AGGACTCTCA TCTAGCCGCC 
AGTGAAGCAG CACTCCTGAG GCCTCGTCCG CGTCCAGGCA ATCTTCGATT GCCCACTGGC 
TCCATTTCAT CAATGAGGAA CCTCGGCAAC GATCAGTGGA AGGTTGTGCG GGGCAATTTC 
TTTATGATCT GCGGCGCGAA CATAACCTGC GCCTGCGCCA GGAGTCCCAA TGGCATCTCC 
CGTTACAGTC ATCTGGGTGA TGGTTGCCTG GACCTGATTC TCGTGAAAAA GACCTCACTG 
CTCAACAACG TGCGTTTTCT GCTCAACACG GCGGGCAGAA GTGGTGATAT TCGCAATTTG 
CCTTTTGTAG AGGTATATCG CACAAGGGAA TTCCGGTTCA GAACTTTTTC TGCCAGCGAG 
GAGGACTACA GCTTGGCAGG GTCTTGTCAG CCGATTACAC CGCCTGAAGA AATGACCGCC 
CACTCCTCCT CGACGGAGTT TTCCAGCTGG AACTGTGATG GAGAGGTGGT GACCGACCTG 
GACATAACCA TGCGATCGCA TTGTCAGCTC ATCGAGGTCT TCATGCGGGG TCCCCATTCC 
TACAGCAAGC CCCTCAAGGG CGGCACCACG CCCACAACCC CAGCTAGCTC TAGCGACAAT 
GTTTACTGCT GCTGCAAGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cerk ( NM_141273.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649530.2 Drosophila melanogaster ceramide kinase, transcript variant B (Cerk), mRNA.

Price & Availability↑

CloneID	OFa03914
Clone ID Related Accession (Same CDS sequence)	NM_169050.2 , NM_141273.3
Accession Version	NM_141273.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ceramide kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141273.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAGA CCAAGCAGCC AGCAGGGCGG TCGTCCACCT CGGCCGGGGA TTTTGTAGCC 
CCCATTAGCT CTACGAGTGC CTCCAAGTTC ACGCCCTGCT CCGCCTCCGA CCACAATGTC 
CTGCTGAACA ACTTCCAGCT TAAGAAGAAG AGCTACAGGG TCCTGCTTCA CGGACAGCAG 
TTGGTTTGGG AGCGTCTGCA GAAGATCAAG CAATCGCCCC AAGGAAACGA GGCCAAGGCA 
CCGTTGCCGC CGGACTCGCC AGCTCAACCA GGCGGAATCT GCTCTTATGG ACCACAAAGC 
CACGTGCTCC ACCTGGACGA TGTGGTTAGC ATAAGGAGCG GGGACACCAA GGCGAGCTCC 
CTCAAACCGC CTTCGCCGCC AGGCTCGGAA CGCAGTTCCG GATGCAGTGG CGATGTTGCT 
CAAGGGAAGC CTACCAGTCA ATACCTGACC ATCAACTATG CCATGAGGCT GAGCAAGTCC 
CAAACGGATT GCAATCGGTG GGAGCTAAGG AGACTCACCT TCTTCAACTC GGATCCCTAC 
ATAGTGAGGC AGTGGGACCA GGAGCTGCAA ATCCGTCTGC ATAGCTCCTC CCCCACCCGG 
ATGCGAGTGC GCCGCCTTTT GGTCTTTATA AACCCCTATG GAGGTCGCAA GGCGGGAGCT 
CAGACCTATG AGCGCCATGT GAGACCTATT TTCCAGCTTG CCGGCGTAGA CGCCACGTGT 
ATCACCACTC AGAGGGCAAA CCAAGTGAAG GACATACTTC TGAGCCATGA TCTGGGAGTA 
TACGATGCGG TTTGCTGTGT CGGAGGCGAT GGCACCGTAG CAGAGGTCAT CAACGGACTG 
ATATTCCGTC AAATGCGAGA GTTGGGACTG GACGAACAGC GGCCACCATA CATTCCAAGA 
CCGGCTCTGC CAGTGGGTGT GATTCCCGCT GGCAGCACCG ACACCATTGC GTATAGTATG 
CACGGCACGG CGGATGTGAG GACAGCGGCT ATCCATGTGA TTCTGGGCCA GCATCGGGGA 
TTGGATGTGT GCAGTGTGAG CAATGGGCAG TCCCTGCTCA GATTCTGTGC CAGTGTCCTG 
AGCTACGGGT ACCTGGGCGA TGTGGCAGCC CAGAGCGAGA ACTACCGCTG GATGGGACCG 
CGCCGGTACG AGTACAGTGG CGTCAAGGCC TTCCTGAATA ATCGCGGCTA TGACGCCGAA 
CTGAGAATGT TAGAAGAGCC CGATCTTCTA CTGACCACGC CGCTGGAGGA CATTCCGCAG 
AGTCCGGATA GTGTGTGCTC GCTGGGAGAG TCAGTGCCAT CCGTCTGCTA TGCCAATTGC 
CAGCGCTGCA GCTTCGCCAG CAGCATACAG GAACAGCGAT CCTCATTGTT CATCCAAGAG 
GAATCTAAAG AGGCAGAGCG CAATCAGCAG GTAGAAACAG AGGACTCTCA TCTAGCCGCC 
AGTGAAGCAG CACTCCTGAG GCCTCGTCCG CGTCCAGGCA ATCTTCGATT GCCCACTGGC 
TCCATTTCAT CAATGAGGAA CCTCGGCAAC GATCAGTGGA AGGTTGTGCG GGGCAATTTC 
TTTATGATCT GCGGCGCGAA CATAACCTGC GCCTGCGCCA GGAGTCCCAA TGGCATCTCC 
CGTTACAGTC ATCTGGGTGA TGGTTGCCTG GACCTGATTC TCGTGAAAAA GACCTCACTG 
CTCAACAACG TGCGTTTTCT GCTCAACACG GCGGGCAGAA GTGGTGATAT TCGCAATTTG 
CCTTTTGTAG AGGTATATCG CACAAGGGAA TTCCGGTTCA GAACTTTTTC TGCCAGCGAG 
GAGGACTACA GCTTGGCAGG GTCTTGTCAG CCGATTACAC CGCCTGAAGA AATGACCGCC 
CACTCCTCCT CGACGGAGTT TTCCAGCTGG AACTGTGATG GAGAGGTGGT GACCGACCTG 
GACATAACCA TGCGATCGCA TTGTCAGCTC ATCGAGGTCT TCATGCGGGG TCCCCATTCC 
TACAGCAAGC CCCTCAAGGG CGGCACCACG CCCACAACCC CAGCTAGCTC TAGCGACAAT 
GTTTACTGCT GCTGCAAGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649530.2
CDS	87..2150
Translation	MTQTKQPAGRSSTSAGDFVAPISSTSASKFTPCSASDHNVLLNNFQLKKKSYRVLLHGQQLVWERLQKIKQSPQGNEAKAPLPPDSPAQPGGICSYGPQSHVLHLDDVVSIRSGDTKASSLKPPSPPGSERSSGCSGDVAQGKPTSQYLTINYAMRLSKSQTDCNRWELRRLTFFNSDPYIVRQWDQELQIRLHSSSPTRMRVRRLLVFINPYGGRKAGAQTYERHVRPIFQLAGVDATCITTQRANQVKDILLSHDLGVYDAVCCVGGDGTVAEVINGLIFRQMRELGLDEQRPPYIPRPALPVGVIPAGSTDTIAYSMHGTADVRTAAIHVILGQHRGLDVCSVSNGQSLLRFCASVLSYGYLGDVAAQSENYRWMGPRRYEYSGVKAFLNNRGYDAELRMLEEPDLLLTTPLEDIPQSPDSVCSLGESVPSVCYANCQRCSFASSIQEQRSSLFIQEESKEAERNQQVETEDSHLAASEAALLRPRPRPGNLRLPTGSISSMRNLGNDQWKVVRGNFFMICGANITCACARSPNGISRYSHLGDGCLDLILVKKTSLLNNVRFLLNTAGRSGDIRNLPFVEVYRTREFRFRTFSASEEDYSLAGSCQPITPPEEMTAHSSSTEFSSWNCDGEVVTDLDITMRSHCQLIEVFMRGPHSYSKPLKGGTTPTTPASSSDNVYCCCKD

Target ORF information:

RefSeq Version	NM_141273.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ceramide kinase, transcript variant B (Cerk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141273.3

ORF Insert Sequence:

ATGACGCAGA CCAAGCAGCC AGCAGGGCGG TCGTCCACCT CGGCCGGGGA TTTTGTAGCC 
CCCATTAGCT CTACGAGTGC CTCCAAGTTC ACGCCCTGCT CCGCCTCCGA CCACAATGTC 
CTGCTGAACA ACTTCCAGCT TAAGAAGAAG AGCTACAGGG TCCTGCTTCA CGGACAGCAG 
TTGGTTTGGG AGCGTCTGCA GAAGATCAAG CAATCGCCCC AAGGAAACGA GGCCAAGGCA 
CCGTTGCCGC CGGACTCGCC AGCTCAACCA GGCGGAATCT GCTCTTATGG ACCACAAAGC 
CACGTGCTCC ACCTGGACGA TGTGGTTAGC ATAAGGAGCG GGGACACCAA GGCGAGCTCC 
CTCAAACCGC CTTCGCCGCC AGGCTCGGAA CGCAGTTCCG GATGCAGTGG CGATGTTGCT 
CAAGGGAAGC CTACCAGTCA ATACCTGACC ATCAACTATG CCATGAGGCT GAGCAAGTCC 
CAAACGGATT GCAATCGGTG GGAGCTAAGG AGACTCACCT TCTTCAACTC GGATCCCTAC 
ATAGTGAGGC AGTGGGACCA GGAGCTGCAA ATCCGTCTGC ATAGCTCCTC CCCCACCCGG 
ATGCGAGTGC GCCGCCTTTT GGTCTTTATA AACCCCTATG GAGGTCGCAA GGCGGGAGCT 
CAGACCTATG AGCGCCATGT GAGACCTATT TTCCAGCTTG CCGGCGTAGA CGCCACGTGT 
ATCACCACTC AGAGGGCAAA CCAAGTGAAG GACATACTTC TGAGCCATGA TCTGGGAGTA 
TACGATGCGG TTTGCTGTGT CGGAGGCGAT GGCACCGTAG CAGAGGTCAT CAACGGACTG 
ATATTCCGTC AAATGCGAGA GTTGGGACTG GACGAACAGC GGCCACCATA CATTCCAAGA 
CCGGCTCTGC CAGTGGGTGT GATTCCCGCT GGCAGCACCG ACACCATTGC GTATAGTATG 
CACGGCACGG CGGATGTGAG GACAGCGGCT ATCCATGTGA TTCTGGGCCA GCATCGGGGA 
TTGGATGTGT GCAGTGTGAG CAATGGGCAG TCCCTGCTCA GATTCTGTGC CAGTGTCCTG 
AGCTACGGGT ACCTGGGCGA TGTGGCAGCC CAGAGCGAGA ACTACCGCTG GATGGGACCG 
CGCCGGTACG AGTACAGTGG CGTCAAGGCC TTCCTGAATA ATCGCGGCTA TGACGCCGAA 
CTGAGAATGT TAGAAGAGCC CGATCTTCTA CTGACCACGC CGCTGGAGGA CATTCCGCAG 
AGTCCGGATA GTGTGTGCTC GCTGGGAGAG TCAGTGCCAT CCGTCTGCTA TGCCAATTGC 
CAGCGCTGCA GCTTCGCCAG CAGCATACAG GAACAGCGAT CCTCATTGTT CATCCAAGAG 
GAATCTAAAG AGGCAGAGCG CAATCAGCAG GTAGAAACAG AGGACTCTCA TCTAGCCGCC 
AGTGAAGCAG CACTCCTGAG GCCTCGTCCG CGTCCAGGCA ATCTTCGATT GCCCACTGGC 
TCCATTTCAT CAATGAGGAA CCTCGGCAAC GATCAGTGGA AGGTTGTGCG GGGCAATTTC 
TTTATGATCT GCGGCGCGAA CATAACCTGC GCCTGCGCCA GGAGTCCCAA TGGCATCTCC 
CGTTACAGTC ATCTGGGTGA TGGTTGCCTG GACCTGATTC TCGTGAAAAA GACCTCACTG 
CTCAACAACG TGCGTTTTCT GCTCAACACG GCGGGCAGAA GTGGTGATAT TCGCAATTTG 
CCTTTTGTAG AGGTATATCG CACAAGGGAA TTCCGGTTCA GAACTTTTTC TGCCAGCGAG 
GAGGACTACA GCTTGGCAGG GTCTTGTCAG CCGATTACAC CGCCTGAAGA AATGACCGCC 
CACTCCTCCT CGACGGAGTT TTCCAGCTGG AACTGTGATG GAGAGGTGGT GACCGACCTG 
GACATAACCA TGCGATCGCA TTGTCAGCTC ATCGAGGTCT TCATGCGGGG TCCCCATTCC 
TACAGCAAGC CCCTCAAGGG CGGCACCACG CCCACAACCC CAGCTAGCTC TAGCGACAAT 
GTTTACTGCT GCTGCAAGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cerk cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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