Gnf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnf1
Entrez Gene ID	40607
Full Name	Germ line transcription factor 1
Synonyms	CG1119,DmRFC1,Dmel\CG1119,GTF-1,Gnf-1,Gtf1,ONF-CAACAA,RF-C 140,RFC,RFC1,RfC,RfC140,dRF-Cp140,dRFC140,gnf1,gtf1,rfc1
General protein information	Preferred Names Germ line transcription factor 1 Names CG1119 gene product from transcript CG1119-RB CG1119-PA CG1119-PB Gnf1-PA Gnf1-PB Replication-factor-C 140kD subunit germ line transcription factor 1 germline transcription factor replication factor C 140kD subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 82E2-82E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_079505.3	NP_524229.1	germ line transcription factor 1, isoform A
NM_001014605.3	NP_001014605.1	germ line transcription factor 1, isoform B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rfc1	NP_445999.1
Xenopus tropicalis (tropical clawed frog)	rfc1	NP_001186496.1
Pan troglodytes (chimpanzee)	RFC1	XP_001140765.1
Saccharomyces cerevisiae (baker's yeast)	RFC1	NP_014860.1
Kluyveromyces lactis	KLLA0D06897g	XP_453368.1
Neurospora crassa	NCU06767	XP_963539.2
	RFC1	XP_001091287.1
Canis lupus familiaris (dog)	RFC1	XP_005618678.1
Gallus gallus (chicken)	RFC1	NP_001006456.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001290	XP_321857.5
Schizosaccharomyces pombe (fission yeast)	rfc1	NP_596607.1
Mus musculus (house mouse)	Rfc1	NP_035388.2
Eremothecium gossypii	AGOS_ACR102W	NP_983504.2
Homo sapiens (human)	RFC1	NP_001191676.1
Bos taurus (cattle)	RFC1	XP_003586244.2
Danio rerio (zebrafish)	rfc1	NP_001082960.2
Drosophila melanogaster (fruit fly)	Gnf1	NP_001014605.1
Magnaporthe oryzae (rice blast fungus)	MGG_03204	XP_003716779.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This paper demonstrated that a putative glycosyltransferase (Gtf2), which is essential for Fap1 glycosylation, readily copurified with another glycosyltransferase (Gtf1) from native S. parasanguinis.
Title: Purification and characterization of an active N-acetylglucosaminyltransferase enzyme complex from Streptococci.

Results indicate that the DNA replication-related element factor (DREF protein) positively regulates the rfc1 promoter.
Title: Transcriptional regulation of the Drosophila rfc1 gene by the DRE-DREF pathway.

The following Gnf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03873	NM_079505.3 Latest version!	Drosophila melanogaster germ line transcription factor 1, transcript variant A (Gnf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03872	NM_001014605.3 Latest version!	Drosophila melanogaster germ line transcription factor 1, transcript variant B (Gnf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gnf1 ( NM_079505.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524229.1 Drosophila melanogaster germ line transcription factor 1, transcript variant A (Gnf1), mRNA.

Price & Availability↑

CloneID	OFa03873
Clone ID Related Accession (Same CDS sequence)	NM_079505.3
Accession Version	NM_079505.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	germ line transcription factor 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079505.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACGCG GCATTGACTC CTTTTTCAAA CGGCTGCCGG CCAAGGCGAA AAGTGCCGAG 
GCCGAAAATG GAGAGACGCC GTCGAAGGCG CCAAAACGCA GGAAAGCCGT GATAATTTCC 
AGCGACGAGG ACGAGGTGGT CAGTCCGCCG GAGACCAAGA AACGCAAGGC TTCGAAGACA 
GCATCCAGCG AGGATGATGT GGTGGCCGCC ACACCGGAAC CGATTGCGAA GAAGGCCAGG 
AACGGGCAGA AACCAGCGCT CTCCAAGCTT AAGCGGCACG TAGATCCTAC GGAGCTTTTC 
GGTGGAGAAA CCAAACGGGT GATTGTGCCC AAGCCGAAGA CCAAAGCTGT TCTCGAGTTC 
GAGAACGAAG ACATTGACCG GAGTTTAATG GAAGTTGATC TGGACGAGAG TATCAAAGAG 
GCTGCCCCCG AAAAAAAGGT TCACTCGATA ACCAGGAGCT CTCCCTCGCC TAAAAGAGCA 
AAGAACAGCA GCCCGGAACC GCCAAAACCC AAGTCAACAA AATCCAAAGC CACCACTCCG 
CGTGTGAAGA AGGAGAAACC TGCGGCAGAT CTAGAATCCA GTGTCCTAAC AGACGAGGAA 
CGCCACGAGC GTAAACGTGC TTCCGCCGTG CTCTACCAGA AGTACAAAAA CCGCAGTTCG 
TGCCTGAACC CCGGCAGCAA AGAAATTCCA AAGGGATCCC CGGATTGTCT GAGTGGCTTA 
ACCTTTGTCG TAACCGGAGT CCTCGAGTCC ATGGAACGAG AGGAGGCGGA GTCCGTGATC 
AAGGAGTATG GCGGAAAGGT GATGACCGTG GTTGGAAAGA AACTCAAGTA TCTGGTCGTA 
GGAGAAGAGG CTGGACCAAA GAAATTAGCT GTGGCTGAGG AACTCAATAT ACCCATTCTC 
AGCGAGGACG GGCTCTTCGA TCTAATCAGG GAGAAATCAG GAATCGCTAA GCAGGTAAAG 
GAAGAGAAGA AAAGCCCGAA GAAGGAGCAC AGCTCCGAGG AGAAAGGGAA AAAGGAAGTC 
AAGACTTCTA GAAGGTCCAG TGATAAAAAG GAGAAGGAAG CCACTAAACT GAAATATGGC 
GAGAAGCATG ATATCGCCAA ACATAAAGTC AAGGAAGAAC ACACTTCCCC GAAGGAGACG 
AAGGATAAGC TTAATGATGT ACCAGCTGTT ACCTTGAAAG TAAAAAAAGA GCCCAGCTCC 
CAGAAAGAAC ACCCTCCGTC GCCAAGAACT GCCGATCTGA AGACTCTGGA TGTGGTCGGG 
ATGGCCTGGG TGGACAAGCA CAAGCCTACG AGCATAAAAG AAATCGTTGG CCAGGCAGGA 
GCCGCCAGCA ACGTGACCAA GCTGATGAAC TGGTTGTCCA AGTGGTATGT TAACCATGAT 
GGAAACAAGA AGCCCCAACG GCCCAATCCT TGGGCCAAGA ACGACGACGG CAGCTTCTAC 
AAGGCGGCCC TACTTTCGGG ACCCCCTGGA ATAGGAAAGA CCACGACGGC GACTCTGGTA 
GTGAAGGAGC TTGGCTTCGA TGCGGTGGAG TTCAACGCCT CCGACACACG CAGCAAACGC 
TTGCTCAAGG ATGAAGTGTC CACGCTGCTG AGCAACAAAT CCCTCTCCGG TTACTTTACA 
GGGCAAGGCC AAGCGGTCTC CCGCAAGCAT GTGCTCATAA TGGACGAAGT TGATGGCATG 
GCCGGTAACG AGGACCGTGG TGGAATGCAG GAGCTGATTG CCTTGATCAA GGATAGCTCT 
ATTCCAATTA TTTGCATGTG TAATGATCGC AACCATCCAA AAATTCGATC CCTGGTTAAC 
TATTGCTACG ATCTGCGATT TCAGAGGCCT CGCCTCGAGC AAATCAAAGG GAAAATAATG 
AGCATCTGCT TTAAGGAAAA AGTAAAGATA TCACCGGCCA AAGTGGAAGA AATCATAGCG 
GCTACCAACA ACGATATTCG GCAATCCATT AACCACATTG CCCTGCTGAG CGCCAAGGAA 
GACGCTTCCC AAAAGTCAGG GCAGCAGGTT GCCACCAAGG ATCTGAAGCT GGGACCCTGG 
GAGGTGGTGC GAAAAGTCTT CACCGCGGAC GAGCACAAAC ACATGTCTTT TGCCGACAAG 
AGCGACCTTT TCTTTCACGA CTACAGCCTG GCCCCGCTCT TTGTGCAGCA AAACTATCTG 
CAGGTCCTAC CACAGGGAAA CAAAAAGGAT GTCCTGGCAA AGGTGGCAGC TACGGCAGAT 
GCCCTAAGCT TGGGTGACCT TGTTGAAAAG CGTATTCGTG CGAATTCTGC TTGGAGTCTT 
CTACCCACTC AGGCCTTTTT CAGCTCGGTC CTTCCTGGCG AGCACATGTG TGGCCACTTC 
ACTGGACAGA TTAACTTTCC CGGGTGGCTG GGCAAGAATT CCAAGTCTGG AAAACGTGCC 
AGGCTGGCCC AGGAGCTGCA TGATCATACT AGGGTCTGCA CCTCGGGCTC AAGGCTATCA 
GTGAGGCTGG ATTACGCCCC CTTCCTTCTG GATAATATTG TGCGACCTCT TGCAAAGGAT 
GGGCAGGAGG GTGTGCCCGC TGCTTTAGAC GTTATGAAGG ATTACCATCT ACTCCGTGAG 
GATCTGGACT CGCTGGTCGA GCTTACATCC TGGCCCGGCA AGAAATCCCC ACTGGACGCA 
GTGGATGGGA GAGTAAAAGC CGCTTTAACG CGATCCTACA ACAAGGAGGT TATGGCATAC 
TCCTATTCCG CTCAAGCAGG CATCAAAAAG AAGAAATCAG AGGCTGCTGG AGCGGATGAC 
GATTACTTGG ACGAGGGTCC GGGCGAAGAG GACGGAGCTG GTGGCCACTT ATCATCAGAG 
GAAGACGAGG ACAAGGACAA CCTAGAGCTG GATAGTTTGA TCAAAGCCAA AAAGAGGACA 
ACTACGTCAA AAGCGTCTGG TGGATCCAAA AAGGCTACAT CTTCCACGGC TTCAAAGTCT 
AAGGCCAAGG CCAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524229.1
CDS	93..3053
Translation	MQRGIDSFFKRLPAKAKSAEAENGETPSKAPKRRKAVIISSDEDEVVSPPETKKRKASKTASSEDDVVAATPEPIAKKARNGQKPALSKLKRHVDPTELFGGETKRVIVPKPKTKAVLEFENEDIDRSLMEVDLDESIKEAAPEKKVHSITRSSPSPKRAKNSSPEPPKPKSTKSKATTPRVKKEKPAADLESSVLTDEERHERKRASAVLYQKYKNRSSCLNPGSKEIPKGSPDCLSGLTFVVTGVLESMEREEAESVIKEYGGKVMTVVGKKLKYLVVGEEAGPKKLAVAEELNIPILSEDGLFDLIREKSGIAKQVKEEKKSPKKEHSSEEKGKKEVKTSRRSSDKKEKEATKLKYGEKHDIAKHKVKEEHTSPKETKDKLNDVPAVTLKVKKEPSSQKEHPPSPRTADLKTLDVVGMAWVDKHKPTSIKEIVGQAGAASNVTKLMNWLSKWYVNHDGNKKPQRPNPWAKNDDGSFYKAALLSGPPGIGKTTTATLVVKELGFDAVEFNASDTRSKRLLKDEVSTLLSNKSLSGYFTGQGQAVSRKHVLIMDEVDGMAGNEDRGGMQELIALIKDSSIPIICMCNDRNHPKIRSLVNYCYDLRFQRPRLEQIKGKIMSICFKEKVKISPAKVEEIIAATNNDIRQSINHIALLSAKEDASQKSGQQVATKDLKLGPWEVVRKVFTADEHKHMSFADKSDLFFHDYSLAPLFVQQNYLQVLPQGNKKDVLAKVAATADALSLGDLVEKRIRANSAWSLLPTQAFFSSVLPGEHMCGHFTGQINFPGWLGKNSKSGKRARLAQELHDHTRVCTSGSRLSVRLDYAPFLLDNIVRPLAKDGQEGVPAALDVMKDYHLLREDLDSLVELTSWPGKKSPLDAVDGRVKAALTRSYNKEVMAYSYSAQAGIKKKKSEAAGADDDYLDEGPGEEDGAGGHLSSEEDEDKDNLELDSLIKAKKRTTTSKASGGSKKATSSTASKSKAKAKK

Target ORF information:

RefSeq Version	NM_079505.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster germ line transcription factor 1, transcript variant A (Gnf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079505.3

ORF Insert Sequence:

ATGCAACGCG GCATTGACTC CTTTTTCAAA CGGCTGCCGG CCAAGGCGAA AAGTGCCGAG 
GCCGAAAATG GAGAGACGCC GTCGAAGGCG CCAAAACGCA GGAAAGCCGT GATAATTTCC 
AGCGACGAGG ACGAGGTGGT CAGTCCGCCG GAGACCAAGA AACGCAAGGC TTCGAAGACA 
GCATCCAGCG AGGATGATGT GGTGGCCGCC ACACCGGAAC CGATTGCGAA GAAGGCCAGG 
AACGGGCAGA AACCAGCGCT CTCCAAGCTT AAGCGGCACG TAGATCCTAC GGAGCTTTTC 
GGTGGAGAAA CCAAACGGGT GATTGTGCCC AAGCCGAAGA CCAAAGCTGT TCTCGAGTTC 
GAGAACGAAG ACATTGACCG GAGTTTAATG GAAGTTGATC TGGACGAGAG TATCAAAGAG 
GCTGCCCCCG AAAAAAAGGT TCACTCGATA ACCAGGAGCT CTCCCTCGCC TAAAAGAGCA 
AAGAACAGCA GCCCGGAACC GCCAAAACCC AAGTCAACAA AATCCAAAGC CACCACTCCG 
CGTGTGAAGA AGGAGAAACC TGCGGCAGAT CTAGAATCCA GTGTCCTAAC AGACGAGGAA 
CGCCACGAGC GTAAACGTGC TTCCGCCGTG CTCTACCAGA AGTACAAAAA CCGCAGTTCG 
TGCCTGAACC CCGGCAGCAA AGAAATTCCA AAGGGATCCC CGGATTGTCT GAGTGGCTTA 
ACCTTTGTCG TAACCGGAGT CCTCGAGTCC ATGGAACGAG AGGAGGCGGA GTCCGTGATC 
AAGGAGTATG GCGGAAAGGT GATGACCGTG GTTGGAAAGA AACTCAAGTA TCTGGTCGTA 
GGAGAAGAGG CTGGACCAAA GAAATTAGCT GTGGCTGAGG AACTCAATAT ACCCATTCTC 
AGCGAGGACG GGCTCTTCGA TCTAATCAGG GAGAAATCAG GAATCGCTAA GCAGGTAAAG 
GAAGAGAAGA AAAGCCCGAA GAAGGAGCAC AGCTCCGAGG AGAAAGGGAA AAAGGAAGTC 
AAGACTTCTA GAAGGTCCAG TGATAAAAAG GAGAAGGAAG CCACTAAACT GAAATATGGC 
GAGAAGCATG ATATCGCCAA ACATAAAGTC AAGGAAGAAC ACACTTCCCC GAAGGAGACG 
AAGGATAAGC TTAATGATGT ACCAGCTGTT ACCTTGAAAG TAAAAAAAGA GCCCAGCTCC 
CAGAAAGAAC ACCCTCCGTC GCCAAGAACT GCCGATCTGA AGACTCTGGA TGTGGTCGGG 
ATGGCCTGGG TGGACAAGCA CAAGCCTACG AGCATAAAAG AAATCGTTGG CCAGGCAGGA 
GCCGCCAGCA ACGTGACCAA GCTGATGAAC TGGTTGTCCA AGTGGTATGT TAACCATGAT 
GGAAACAAGA AGCCCCAACG GCCCAATCCT TGGGCCAAGA ACGACGACGG CAGCTTCTAC 
AAGGCGGCCC TACTTTCGGG ACCCCCTGGA ATAGGAAAGA CCACGACGGC GACTCTGGTA 
GTGAAGGAGC TTGGCTTCGA TGCGGTGGAG TTCAACGCCT CCGACACACG CAGCAAACGC 
TTGCTCAAGG ATGAAGTGTC CACGCTGCTG AGCAACAAAT CCCTCTCCGG TTACTTTACA 
GGGCAAGGCC AAGCGGTCTC CCGCAAGCAT GTGCTCATAA TGGACGAAGT TGATGGCATG 
GCCGGTAACG AGGACCGTGG TGGAATGCAG GAGCTGATTG CCTTGATCAA GGATAGCTCT 
ATTCCAATTA TTTGCATGTG TAATGATCGC AACCATCCAA AAATTCGATC CCTGGTTAAC 
TATTGCTACG ATCTGCGATT TCAGAGGCCT CGCCTCGAGC AAATCAAAGG GAAAATAATG 
AGCATCTGCT TTAAGGAAAA AGTAAAGATA TCACCGGCCA AAGTGGAAGA AATCATAGCG 
GCTACCAACA ACGATATTCG GCAATCCATT AACCACATTG CCCTGCTGAG CGCCAAGGAA 
GACGCTTCCC AAAAGTCAGG GCAGCAGGTT GCCACCAAGG ATCTGAAGCT GGGACCCTGG 
GAGGTGGTGC GAAAAGTCTT CACCGCGGAC GAGCACAAAC ACATGTCTTT TGCCGACAAG 
AGCGACCTTT TCTTTCACGA CTACAGCCTG GCCCCGCTCT TTGTGCAGCA AAACTATCTG 
CAGGTCCTAC CACAGGGAAA CAAAAAGGAT GTCCTGGCAA AGGTGGCAGC TACGGCAGAT 
GCCCTAAGCT TGGGTGACCT TGTTGAAAAG CGTATTCGTG CGAATTCTGC TTGGAGTCTT 
CTACCCACTC AGGCCTTTTT CAGCTCGGTC CTTCCTGGCG AGCACATGTG TGGCCACTTC 
ACTGGACAGA TTAACTTTCC CGGGTGGCTG GGCAAGAATT CCAAGTCTGG AAAACGTGCC 
AGGCTGGCCC AGGAGCTGCA TGATCATACT AGGGTCTGCA CCTCGGGCTC AAGGCTATCA 
GTGAGGCTGG ATTACGCCCC CTTCCTTCTG GATAATATTG TGCGACCTCT TGCAAAGGAT 
GGGCAGGAGG GTGTGCCCGC TGCTTTAGAC GTTATGAAGG ATTACCATCT ACTCCGTGAG 
GATCTGGACT CGCTGGTCGA GCTTACATCC TGGCCCGGCA AGAAATCCCC ACTGGACGCA 
GTGGATGGGA GAGTAAAAGC CGCTTTAACG CGATCCTACA ACAAGGAGGT TATGGCATAC 
TCCTATTCCG CTCAAGCAGG CATCAAAAAG AAGAAATCAG AGGCTGCTGG AGCGGATGAC 
GATTACTTGG ACGAGGGTCC GGGCGAAGAG GACGGAGCTG GTGGCCACTT ATCATCAGAG 
GAAGACGAGG ACAAGGACAA CCTAGAGCTG GATAGTTTGA TCAAAGCCAA AAAGAGGACA 
ACTACGTCAA AAGCGTCTGG TGGATCCAAA AAGGCTACAT CTTCCACGGC TTCAAAGTCT 
AAGGCCAAGG CCAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnf1 ( NM_001014605.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014605.1 Drosophila melanogaster germ line transcription factor 1, transcript variant B (Gnf1), mRNA.

Price & Availability↑

CloneID	OFa03872
Clone ID Related Accession (Same CDS sequence)	NM_001014605.3
Accession Version	NM_001014605.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	germ line transcription factor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001014605.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACGCG GCATTGACTC CTTTTTCAAA CGGCTGCCGG CCAAGGCGAA AAGTGCCGAG 
GCCGAAAATG GAGAGACGCC GTCGAAGGCG CCAAAACGCA GGAAAGCCGT GATAATTTCC 
AGCGACGAGG ACGAGGTGGT CAGTCCGCCG GAGACCAAGA AACGCAAGGC TTCGAAGACA 
GCATCCAGCG AGGATGATGT GGTGGCCGCC ACACCGGAAC CGATTGCGAA GAAGGCCAGG 
AACGGGCAGA AACCAGCGCT CTCCAAGCTT AAGCGGCACG TAGATCCTAC GGAGCTTTTC 
GGTGGAGAAA CCAAACGGGT GATTGTGCCC AAGCCGAAGA CCAAAGCTGT TCTCGAGTTC 
GAGAACGAAG ACATTGACCG GAGTTTAATG GAAGTTGATC TGGACGAGAG TATCAAAGAG 
GCTGCCCCCG AAAAAAAGGT TCACTCGATA ACCAGGAGCT CTCCCTCGCC TAAAAGAGCA 
AAGAACAGCA GCCCGGAACC GCCAAAACCC AAGTCAACAA AATCCAAAGC CACCACTCCG 
CGTGTGAAGA AGGAGAAACC TGCGGCAGAT CTAGAATCCA GTGTCCTAAC AGACGAGGAA 
CGCCACGAGC GTAAACGTGC TTCCGCCGTG CTCTACCAGA AGTACAAAAA CCGCAGTTCG 
TGCCTGAACC CCGGCAGCAA AGAAATTCCA AAGGGATCCC CGGATTGTCT GAGTGGCTTA 
ACCTTTGTCG TAACCGGAGT CCTCGAGTCC ATGGAACGAG AGGAGGCGGA GTCCGTGATC 
AAGGAGTATG GCGGAAAGGT GATGACCGTG GTTGGAAAGA AACTCAAGTA TCTGGTCGTA 
GGAGAAGAGG CTGGACCAAA GAAATTAGCT GTGGCTGAGG AACTCAATAT ACCCATTCTC 
AGCGAGGACG GGCTCTTCGA TCTAATCAGG GAGAAATCAG GAATCGCTAA GCAGGTAAAG 
GAAGAGAAGA AAAGCCCGAA GAAGGAGCAC AGCTCCGAGG AGAAAGGGAA AAAGGAAGTC 
AAGACTTCTA GAAGGTCCAG TGATAAAAAG GAGAAGGAAG CCACTAAACT GAAATATGGC 
GAGAAGCATG ATATCGCCAA ACATAAAGTC AAGGAAGAAC ACACTTCCCC GAAGGAGACG 
AAGGATAAGC TTAATGATGT ACCAGCTGTT ACCTTGAAAG TAAAAAAAGA GCCCAGCTCC 
CAGAAAGAAC ACCCTCCGTC GCCAAGAACT GCCGATCTGA AGACTCTGGA TGTGGTCGGG 
ATGGCCTGGG TGGACAAGCA CAAGCCTACG AGCATAAAAG AAATCGTTGG CCAGGCAGGA 
GCCGCCAGCA ACGTGACCAA TCACAATTTG AAACTGAAAG CCAAACAGGA GAGGGTTAAA 
GTTTTACATT ATTTTAATTT TCCTAGGCTG ATGAACTGGT TGTCCAAGTG GTATGTTAAC 
CATGATGGAA ACAAGAAGCC CCAACGGCCC AATCCTTGGG CCAAGAACGA CGACGGCAGC 
TTCTACAAGG CGGCCCTACT TTCGGGACCC CCTGGAATAG GAAAGACCAC GACGGCGACT 
CTGGTAGTGA AGGAGCTTGG CTTCGATGCG GTGGAGTTCA ACGCCTCCGA CACACGCAGC 
AAACGCTTGC TCAAGGATGA AGTGTCCACG CTGCTGAGCA ACAAATCCCT CTCCGGTTAC 
TTTACAGGGC AAGGCCAAGC GGTCTCCCGC AAGCATGTGC TCATAATGGA CGAAGTTGAT 
GGCATGGCCG GTAACGAGGA CCGTGGTGGA ATGCAGGAGC TGATTGCCTT GATCAAGGAT 
AGCTCTATTC CAATTATTTG CATGTGTAAT GATCGCAACC ATCCAAAAAT TCGATCCCTG 
GTTAACTATT GCTACGATCT GCGATTTCAG AGGCCTCGCC TCGAGCAAAT CAAAGGGAAA 
ATAATGAGCA TCTGCTTTAA GGAAAAAGTA AAGATATCAC CGGCCAAAGT GGAAGAAATC 
ATAGCGGCTA CCAACAACGA TATTCGGCAA TCCATTAACC ACATTGCCCT GCTGAGCGCC 
AAGGAAGACG CTTCCCAAAA GTCAGGGCAG CAGGTTGCCA CCAAGGATCT GAAGCTGGGA 
CCCTGGGAGG TGGTGCGAAA AGTCTTCACC GCGGACGAGC ACAAACACAT GTCTTTTGCC 
GACAAGAGCG ACCTTTTCTT TCACGACTAC AGCCTGGCCC CGCTCTTTGT GCAGCAAAAC 
TATCTGCAGG TCCTACCACA GGGAAACAAA AAGGATGTCC TGGCAAAGGT GGCAGCTACG 
GCAGATGCCC TAAGCTTGGG TGACCTTGTT GAAAAGCGTA TTCGTGCGAA TTCTGCTTGG 
AGTCTTCTAC CCACTCAGGC CTTTTTCAGC TCGGTCCTTC CTGGCGAGCA CATGTGTGGC 
CACTTCACTG GACAGATTAA CTTTCCCGGG TGGCTGGGCA AGAATTCCAA GTCTGGAAAA 
CGTGCCAGGC TGGCCCAGGA GCTGCATGAT CATACTAGGG TCTGCACCTC GGGCTCAAGG 
CTATCAGTGA GGCTGGATTA CGCCCCCTTC CTTCTGGATA ATATTGTGCG ACCTCTTGCA 
AAGGATGGGC AGGAGGGTGT GCCCGCTGCT TTAGACGTTA TGAAGGATTA CCATCTACTC 
CGTGAGGATC TGGACTCGCT GGTCGAGCTT ACATCCTGGC CCGGCAAGAA ATCCCCACTG 
GACGCAGTGG ATGGGAGAGT AAAAGCCGCT TTAACGCGAT CCTACAACAA GGAGGTTATG 
GCATACTCCT ATTCCGCTCA AGCAGGCATC AAAAAGAAGA AATCAGAGGC TGCTGGAGCG 
GATGACGATT ACTTGGACGA GGGTCCGGGC GAAGAGGACG GAGCTGGTGG CCACTTATCA 
TCAGAGGAAG ACGAGGACAA GGACAACCTA GAGCTGGATA GTTTGATCAA AGCCAAAAAG 
AGGACAACTA CGTCAAAAGC GTCTGGTGGA TCCAAAAAGG CTACATCTTC CACGGCTTCA 
AAGTCTAAGG CCAAGGCCAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014605.1
CDS	93..3119
Translation	MQRGIDSFFKRLPAKAKSAEAENGETPSKAPKRRKAVIISSDEDEVVSPPETKKRKASKTASSEDDVVAATPEPIAKKARNGQKPALSKLKRHVDPTELFGGETKRVIVPKPKTKAVLEFENEDIDRSLMEVDLDESIKEAAPEKKVHSITRSSPSPKRAKNSSPEPPKPKSTKSKATTPRVKKEKPAADLESSVLTDEERHERKRASAVLYQKYKNRSSCLNPGSKEIPKGSPDCLSGLTFVVTGVLESMEREEAESVIKEYGGKVMTVVGKKLKYLVVGEEAGPKKLAVAEELNIPILSEDGLFDLIREKSGIAKQVKEEKKSPKKEHSSEEKGKKEVKTSRRSSDKKEKEATKLKYGEKHDIAKHKVKEEHTSPKETKDKLNDVPAVTLKVKKEPSSQKEHPPSPRTADLKTLDVVGMAWVDKHKPTSIKEIVGQAGAASNVTNHNLKLKAKQERVKVLHYFNFPRLMNWLSKWYVNHDGNKKPQRPNPWAKNDDGSFYKAALLSGPPGIGKTTTATLVVKELGFDAVEFNASDTRSKRLLKDEVSTLLSNKSLSGYFTGQGQAVSRKHVLIMDEVDGMAGNEDRGGMQELIALIKDSSIPIICMCNDRNHPKIRSLVNYCYDLRFQRPRLEQIKGKIMSICFKEKVKISPAKVEEIIAATNNDIRQSINHIALLSAKEDASQKSGQQVATKDLKLGPWEVVRKVFTADEHKHMSFADKSDLFFHDYSLAPLFVQQNYLQVLPQGNKKDVLAKVAATADALSLGDLVEKRIRANSAWSLLPTQAFFSSVLPGEHMCGHFTGQINFPGWLGKNSKSGKRARLAQELHDHTRVCTSGSRLSVRLDYAPFLLDNIVRPLAKDGQEGVPAALDVMKDYHLLREDLDSLVELTSWPGKKSPLDAVDGRVKAALTRSYNKEVMAYSYSAQAGIKKKKSEAAGADDDYLDEGPGEEDGAGGHLSSEEDEDKDNLELDSLIKAKKRTTTSKASGGSKKATSSTASKSKAKAKK

Target ORF information:

RefSeq Version	NM_001014605.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster germ line transcription factor 1, transcript variant B (Gnf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014605.3

ORF Insert Sequence:

ATGCAACGCG GCATTGACTC CTTTTTCAAA CGGCTGCCGG CCAAGGCGAA AAGTGCCGAG 
GCCGAAAATG GAGAGACGCC GTCGAAGGCG CCAAAACGCA GGAAAGCCGT GATAATTTCC 
AGCGACGAGG ACGAGGTGGT CAGTCCGCCG GAGACCAAGA AACGCAAGGC TTCGAAGACA 
GCATCCAGCG AGGATGATGT GGTGGCCGCC ACACCGGAAC CGATTGCGAA GAAGGCCAGG 
AACGGGCAGA AACCAGCGCT CTCCAAGCTT AAGCGGCACG TAGATCCTAC GGAGCTTTTC 
GGTGGAGAAA CCAAACGGGT GATTGTGCCC AAGCCGAAGA CCAAAGCTGT TCTCGAGTTC 
GAGAACGAAG ACATTGACCG GAGTTTAATG GAAGTTGATC TGGACGAGAG TATCAAAGAG 
GCTGCCCCCG AAAAAAAGGT TCACTCGATA ACCAGGAGCT CTCCCTCGCC TAAAAGAGCA 
AAGAACAGCA GCCCGGAACC GCCAAAACCC AAGTCAACAA AATCCAAAGC CACCACTCCG 
CGTGTGAAGA AGGAGAAACC TGCGGCAGAT CTAGAATCCA GTGTCCTAAC AGACGAGGAA 
CGCCACGAGC GTAAACGTGC TTCCGCCGTG CTCTACCAGA AGTACAAAAA CCGCAGTTCG 
TGCCTGAACC CCGGCAGCAA AGAAATTCCA AAGGGATCCC CGGATTGTCT GAGTGGCTTA 
ACCTTTGTCG TAACCGGAGT CCTCGAGTCC ATGGAACGAG AGGAGGCGGA GTCCGTGATC 
AAGGAGTATG GCGGAAAGGT GATGACCGTG GTTGGAAAGA AACTCAAGTA TCTGGTCGTA 
GGAGAAGAGG CTGGACCAAA GAAATTAGCT GTGGCTGAGG AACTCAATAT ACCCATTCTC 
AGCGAGGACG GGCTCTTCGA TCTAATCAGG GAGAAATCAG GAATCGCTAA GCAGGTAAAG 
GAAGAGAAGA AAAGCCCGAA GAAGGAGCAC AGCTCCGAGG AGAAAGGGAA AAAGGAAGTC 
AAGACTTCTA GAAGGTCCAG TGATAAAAAG GAGAAGGAAG CCACTAAACT GAAATATGGC 
GAGAAGCATG ATATCGCCAA ACATAAAGTC AAGGAAGAAC ACACTTCCCC GAAGGAGACG 
AAGGATAAGC TTAATGATGT ACCAGCTGTT ACCTTGAAAG TAAAAAAAGA GCCCAGCTCC 
CAGAAAGAAC ACCCTCCGTC GCCAAGAACT GCCGATCTGA AGACTCTGGA TGTGGTCGGG 
ATGGCCTGGG TGGACAAGCA CAAGCCTACG AGCATAAAAG AAATCGTTGG CCAGGCAGGA 
GCCGCCAGCA ACGTGACCAA TCACAATTTG AAACTGAAAG CCAAACAGGA GAGGGTTAAA 
GTTTTACATT ATTTTAATTT TCCTAGGCTG ATGAACTGGT TGTCCAAGTG GTATGTTAAC 
CATGATGGAA ACAAGAAGCC CCAACGGCCC AATCCTTGGG CCAAGAACGA CGACGGCAGC 
TTCTACAAGG CGGCCCTACT TTCGGGACCC CCTGGAATAG GAAAGACCAC GACGGCGACT 
CTGGTAGTGA AGGAGCTTGG CTTCGATGCG GTGGAGTTCA ACGCCTCCGA CACACGCAGC 
AAACGCTTGC TCAAGGATGA AGTGTCCACG CTGCTGAGCA ACAAATCCCT CTCCGGTTAC 
TTTACAGGGC AAGGCCAAGC GGTCTCCCGC AAGCATGTGC TCATAATGGA CGAAGTTGAT 
GGCATGGCCG GTAACGAGGA CCGTGGTGGA ATGCAGGAGC TGATTGCCTT GATCAAGGAT 
AGCTCTATTC CAATTATTTG CATGTGTAAT GATCGCAACC ATCCAAAAAT TCGATCCCTG 
GTTAACTATT GCTACGATCT GCGATTTCAG AGGCCTCGCC TCGAGCAAAT CAAAGGGAAA 
ATAATGAGCA TCTGCTTTAA GGAAAAAGTA AAGATATCAC CGGCCAAAGT GGAAGAAATC 
ATAGCGGCTA CCAACAACGA TATTCGGCAA TCCATTAACC ACATTGCCCT GCTGAGCGCC 
AAGGAAGACG CTTCCCAAAA GTCAGGGCAG CAGGTTGCCA CCAAGGATCT GAAGCTGGGA 
CCCTGGGAGG TGGTGCGAAA AGTCTTCACC GCGGACGAGC ACAAACACAT GTCTTTTGCC 
GACAAGAGCG ACCTTTTCTT TCACGACTAC AGCCTGGCCC CGCTCTTTGT GCAGCAAAAC 
TATCTGCAGG TCCTACCACA GGGAAACAAA AAGGATGTCC TGGCAAAGGT GGCAGCTACG 
GCAGATGCCC TAAGCTTGGG TGACCTTGTT GAAAAGCGTA TTCGTGCGAA TTCTGCTTGG 
AGTCTTCTAC CCACTCAGGC CTTTTTCAGC TCGGTCCTTC CTGGCGAGCA CATGTGTGGC 
CACTTCACTG GACAGATTAA CTTTCCCGGG TGGCTGGGCA AGAATTCCAA GTCTGGAAAA 
CGTGCCAGGC TGGCCCAGGA GCTGCATGAT CATACTAGGG TCTGCACCTC GGGCTCAAGG 
CTATCAGTGA GGCTGGATTA CGCCCCCTTC CTTCTGGATA ATATTGTGCG ACCTCTTGCA 
AAGGATGGGC AGGAGGGTGT GCCCGCTGCT TTAGACGTTA TGAAGGATTA CCATCTACTC 
CGTGAGGATC TGGACTCGCT GGTCGAGCTT ACATCCTGGC CCGGCAAGAA ATCCCCACTG 
GACGCAGTGG ATGGGAGAGT AAAAGCCGCT TTAACGCGAT CCTACAACAA GGAGGTTATG 
GCATACTCCT ATTCCGCTCA AGCAGGCATC AAAAAGAAGA AATCAGAGGC TGCTGGAGCG 
GATGACGATT ACTTGGACGA GGGTCCGGGC GAAGAGGACG GAGCTGGTGG CCACTTATCA 
TCAGAGGAAG ACGAGGACAA GGACAACCTA GAGCTGGATA GTTTGATCAA AGCCAAAAAG 
AGGACAACTA CGTCAAAAGC GTCTGGTGGA TCCAAAAAGG CTACATCTTC CACGGCTTCA 
AAGTCTAAGG CCAAGGCCAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gnf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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