Nep2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Nep2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nep2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa03853 NM_079503.4
Latest version!
Drosophila melanogaster neprilysin 2, transcript variant A (Nep2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa03853 NM_001275331.1
Latest version!
Drosophila melanogaster neprilysin 2, transcript variant C (Nep2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa08059 NM_001259985.2
Latest version!
Drosophila melanogaster neprilysin 2, transcript variant B (Nep2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa03853
    Clone ID Related Accession (Same CDS sequence) NM_001275331.1 , NM_079503.4
    Accession Version NM_001275331.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neprilysin 2, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGACGG TAATCCAAAA CCCCAACTGG TGGCGTCGCC GTAACAAATT GGAAAAAAGT 
    CTGCTGGTCT CGCTGGGCAT CATGTTCGTG GTGCTTGCTA CCGGGTTCGG TCTCTGGATT
    GGCAAAGTGC TGAGGACGAG TCCCCCGTCG AATCCCCAGG CCACTGCTCT CCACGGCGAC
    AGCACCACCA TCAACCAGGT TCCAACGGGC ACCGCCTCGA AGGGCAAGTC GGGCGATTCG
    GGCGACGTGT GCCTCACCCA GGAGTGCATC CACACGGCTT CCACCGTTTT GCGGAAGATG
    AAGCCCGAGG TAGAGCCGTG CGACAACTTT TACGAGTTCG CCTGCGGCAC ATACCTTGAG
    GAGGAGAACA TCCCCGACGA CAAGGTCTCC ATCAGCACCT TCTCCGTGAT CTCCGACAAG
    CTGCAGGAGC AGTTGAAGGA CATCATCACC GCGGAGCGAC CGGAAACGGA GCCGAAGCAC
    TTCCGACTGC CCAACCTGCT TTACAAGGCC TGCATGAACA AAACTCTGAT CGAGACCCTG
    GGCCCTGAGC CCATAACTCG CGTGGCCGAG AGGCTGGGCG GCTGGCCGCT GATCAAGGGA
    GACTCTTGGA ACGCGGACGA CAGCTGGACT TGGCAGGAGC AGGTCAAGAA GTTCCGCACC
    GCCGGTTTTA GCATGGACTA CATCATCGAC TTTTCAATCG GAGTGGATTT GCAGAACAGC
    ACTAAGCGGC TTATAGATCT GGACCAGTCC TCCCTGGCCT TGAGTCGCGA GTACCTCGTG
    AAAGGATTCA ATGAGACGCT GGTGACGGCA TACTACAAGT ATATGGTTGA CATCGCGGTT
    CTTTTCGGAG CAAATAGGGA CTTGGCCAAG ACGGAGCTGC TGTTATCTTT GGAGTTCGAG
    ATGGCGCTGG CCAATATCTC CTGGCCCAAC GAGAAGCGAC GCAACTCCTC TGAGCTGTAC
    AATCTAAGAA CTCCAGCGCA GCTCCAGGCC GCCTACCCCT ACGTGCAGTG GGTGGACTAC
    ATGAACGCCC TGCTGCCGGA GGGACTGAAC GTGGCCGAGG ACGAGATGAT CAACCTGTCG
    GTGCCCAGCT TCTTCGAGGA CCTTGGCAAG CTGCTGGCCA AGACGCCGAA GCGTGTCATC
    GCCAACTATA TGTTCTGGCG TATCCACGGC TTCTCCGTGG GATTTCTGAG TGAGGAGTTC
    CGCAAGCGAC AGCTGCAGTA CGCCACAGCC CTTTCGGGGC GCCAGGAACA GGAGGCTAGA
    TGGAAGGAGT GCGTCGACAT CGCCACCAGC AGTCTTGGCA TTTCCGTGGG ATCGCTCTAC
    GTGGGCAAGC ACTTCCACAA GGATTCGAAG GCCAACGCCC TGGAGATGGT CAACGAAATC
    CGCAACGTGT TCAACGACAT CCTGGACGAG GTCAACTGGA TGGACGCCAA GACGAAGAAG
    GAGGCCAAGC TAAAGCTGCA CAGCATGGCC ACCCACATCG GCTATCCGGA CGAGATGCTC
    GACAACGAGA AGCTGGCCGC CTACTACGCC AAGCTCGACA TCGATCCCGA CAAGTACTTC
    GAGTCGTTCC TGGGCATGAA CATATTTGGC ACGGATTACT CGTTCAACAA GCTTCGTTTG
    CCGGTCAACA AGACGGACTG GGTTCGCCAC GCTCGTCCGG CGATCGTGAA CGCGTTCTAC
    TCGTCCTTAG AGAACAGTAT TCAATTCCCG GCCGGCATCC TGCAGGGGCA CTTCTTCAAC
    GCCCAACGTC CGAAGTACAT GAACTTTGGC GCCATTGGCT ACGTGATTGG TCACGAGATC
    ACCCATGGTT TCGACGACCA GGGTCGCCAG TTCGACGTGA AGGGCAACCT GCGGGACTGG
    TGGCACCCGG ACACCCAGAA GGCCTATCTG GCCAAGGCGA AGTGCATCAT TGAGCAGTAC
    GGCAACTACA CCGAAAGGGC CACGGGATTG AACTTGAATG GCATCAACAC CCAGGGGGAG
    AATATCGCCG ACAATGGTGG CGTCAAGGAG TCATACATCG CCTACCGCAG ATGGGCTGAG
    AAGCACGGAC CGGAAGCAAA GCTTCCGGGA TTGGACTACA CCCCGGAGCA GATGTTCTGG
    GTGGCAGCCG GACAGACTTG GTGTGCTAAG TACCGAAAAG AATCTCTCAA GATGCGTATT
    ACTACCGGCG TGCATTCTCC ATCCGAGTTC CGAGTCCTCG GGTCGCTCAG CAACATGAAG
    GACTTCGCCA AGGACTTCCA TTGTCCAGAG GGATCACCCA TGAATCCGGT CCAAAAGTGC
    GAGGTTTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262260.1
    CDS317..2608
    Translation

    Target ORF information:

    RefSeq Version NM_001275331.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neprilysin 2, transcript variant C (Nep2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275331.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGACGG TAATCCAAAA CCCCAACTGG TGGCGTCGCC GTAACAAATT GGAAAAAAGT 
    CTGCTGGTCT CGCTGGGCAT CATGTTCGTG GTGCTTGCTA CCGGGTTCGG TCTCTGGATT
    GGCAAAGTGC TGAGGACGAG TCCCCCGTCG AATCCCCAGG CCACTGCTCT CCACGGCGAC
    AGCACCACCA TCAACCAGGT TCCAACGGGC ACCGCCTCGA AGGGCAAGTC GGGCGATTCG
    GGCGACGTGT GCCTCACCCA GGAGTGCATC CACACGGCTT CCACCGTTTT GCGGAAGATG
    AAGCCCGAGG TAGAGCCGTG CGACAACTTT TACGAGTTCG CCTGCGGCAC ATACCTTGAG
    GAGGAGAACA TCCCCGACGA CAAGGTCTCC ATCAGCACCT TCTCCGTGAT CTCCGACAAG
    CTGCAGGAGC AGTTGAAGGA CATCATCACC GCGGAGCGAC CGGAAACGGA GCCGAAGCAC
    TTCCGACTGC CCAACCTGCT TTACAAGGCC TGCATGAACA AAACTCTGAT CGAGACCCTG
    GGCCCTGAGC CCATAACTCG CGTGGCCGAG AGGCTGGGCG GCTGGCCGCT GATCAAGGGA
    GACTCTTGGA ACGCGGACGA CAGCTGGACT TGGCAGGAGC AGGTCAAGAA GTTCCGCACC
    GCCGGTTTTA GCATGGACTA CATCATCGAC TTTTCAATCG GAGTGGATTT GCAGAACAGC
    ACTAAGCGGC TTATAGATCT GGACCAGTCC TCCCTGGCCT TGAGTCGCGA GTACCTCGTG
    AAAGGATTCA ATGAGACGCT GGTGACGGCA TACTACAAGT ATATGGTTGA CATCGCGGTT
    CTTTTCGGAG CAAATAGGGA CTTGGCCAAG ACGGAGCTGC TGTTATCTTT GGAGTTCGAG
    ATGGCGCTGG CCAATATCTC CTGGCCCAAC GAGAAGCGAC GCAACTCCTC TGAGCTGTAC
    AATCTAAGAA CTCCAGCGCA GCTCCAGGCC GCCTACCCCT ACGTGCAGTG GGTGGACTAC
    ATGAACGCCC TGCTGCCGGA GGGACTGAAC GTGGCCGAGG ACGAGATGAT CAACCTGTCG
    GTGCCCAGCT TCTTCGAGGA CCTTGGCAAG CTGCTGGCCA AGACGCCGAA GCGTGTCATC
    GCCAACTATA TGTTCTGGCG TATCCACGGC TTCTCCGTGG GATTTCTGAG TGAGGAGTTC
    CGCAAGCGAC AGCTGCAGTA CGCCACAGCC CTTTCGGGGC GCCAGGAACA GGAGGCTAGA
    TGGAAGGAGT GCGTCGACAT CGCCACCAGC AGTCTTGGCA TTTCCGTGGG ATCGCTCTAC
    GTGGGCAAGC ACTTCCACAA GGATTCGAAG GCCAACGCCC TGGAGATGGT CAACGAAATC
    CGCAACGTGT TCAACGACAT CCTGGACGAG GTCAACTGGA TGGACGCCAA GACGAAGAAG
    GAGGCCAAGC TAAAGCTGCA CAGCATGGCC ACCCACATCG GCTATCCGGA CGAGATGCTC
    GACAACGAGA AGCTGGCCGC CTACTACGCC AAGCTCGACA TCGATCCCGA CAAGTACTTC
    GAGTCGTTCC TGGGCATGAA CATATTTGGC ACGGATTACT CGTTCAACAA GCTTCGTTTG
    CCGGTCAACA AGACGGACTG GGTTCGCCAC GCTCGTCCGG CGATCGTGAA CGCGTTCTAC
    TCGTCCTTAG AGAACAGTAT TCAATTCCCG GCCGGCATCC TGCAGGGGCA CTTCTTCAAC
    GCCCAACGTC CGAAGTACAT GAACTTTGGC GCCATTGGCT ACGTGATTGG TCACGAGATC
    ACCCATGGTT TCGACGACCA GGGTCGCCAG TTCGACGTGA AGGGCAACCT GCGGGACTGG
    TGGCACCCGG ACACCCAGAA GGCCTATCTG GCCAAGGCGA AGTGCATCAT TGAGCAGTAC
    GGCAACTACA CCGAAAGGGC CACGGGATTG AACTTGAATG GCATCAACAC CCAGGGGGAG
    AATATCGCCG ACAATGGTGG CGTCAAGGAG TCATACATCG CCTACCGCAG ATGGGCTGAG
    AAGCACGGAC CGGAAGCAAA GCTTCCGGGA TTGGACTACA CCCCGGAGCA GATGTTCTGG
    GTGGCAGCCG GACAGACTTG GTGTGCTAAG TACCGAAAAG AATCTCTCAA GATGCGTATT
    ACTACCGGCG TGCATTCTCC ATCCGAGTTC CGAGTCCTCG GGTCGCTCAG CAACATGAAG
    GACTTCGCCA AGGACTTCCA TTGTCCAGAG GGATCACCCA TGAATCCGGT CCAAAAGTGC
    GAGGTTTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03853
    Clone ID Related Accession (Same CDS sequence) NM_001275331.1 , NM_079503.4
    Accession Version NM_079503.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neprilysin 2, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079503.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGACGG TAATCCAAAA CCCCAACTGG TGGCGTCGCC GTAACAAATT GGAAAAAAGT 
    CTGCTGGTCT CGCTGGGCAT CATGTTCGTG GTGCTTGCTA CCGGGTTCGG TCTCTGGATT
    GGCAAAGTGC TGAGGACGAG TCCCCCGTCG AATCCCCAGG CCACTGCTCT CCACGGCGAC
    AGCACCACCA TCAACCAGGT TCCAACGGGC ACCGCCTCGA AGGGCAAGTC GGGCGATTCG
    GGCGACGTGT GCCTCACCCA GGAGTGCATC CACACGGCTT CCACCGTTTT GCGGAAGATG
    AAGCCCGAGG TAGAGCCGTG CGACAACTTT TACGAGTTCG CCTGCGGCAC ATACCTTGAG
    GAGGAGAACA TCCCCGACGA CAAGGTCTCC ATCAGCACCT TCTCCGTGAT CTCCGACAAG
    CTGCAGGAGC AGTTGAAGGA CATCATCACC GCGGAGCGAC CGGAAACGGA GCCGAAGCAC
    TTCCGACTGC CCAACCTGCT TTACAAGGCC TGCATGAACA AAACTCTGAT CGAGACCCTG
    GGCCCTGAGC CCATAACTCG CGTGGCCGAG AGGCTGGGCG GCTGGCCGCT GATCAAGGGA
    GACTCTTGGA ACGCGGACGA CAGCTGGACT TGGCAGGAGC AGGTCAAGAA GTTCCGCACC
    GCCGGTTTTA GCATGGACTA CATCATCGAC TTTTCAATCG GAGTGGATTT GCAGAACAGC
    ACTAAGCGGC TTATAGATCT GGACCAGTCC TCCCTGGCCT TGAGTCGCGA GTACCTCGTG
    AAAGGATTCA ATGAGACGCT GGTGACGGCA TACTACAAGT ATATGGTTGA CATCGCGGTT
    CTTTTCGGAG CAAATAGGGA CTTGGCCAAG ACGGAGCTGC TGTTATCTTT GGAGTTCGAG
    ATGGCGCTGG CCAATATCTC CTGGCCCAAC GAGAAGCGAC GCAACTCCTC TGAGCTGTAC
    AATCTAAGAA CTCCAGCGCA GCTCCAGGCC GCCTACCCCT ACGTGCAGTG GGTGGACTAC
    ATGAACGCCC TGCTGCCGGA GGGACTGAAC GTGGCCGAGG ACGAGATGAT CAACCTGTCG
    GTGCCCAGCT TCTTCGAGGA CCTTGGCAAG CTGCTGGCCA AGACGCCGAA GCGTGTCATC
    GCCAACTATA TGTTCTGGCG TATCCACGGC TTCTCCGTGG GATTTCTGAG TGAGGAGTTC
    CGCAAGCGAC AGCTGCAGTA CGCCACAGCC CTTTCGGGGC GCCAGGAACA GGAGGCTAGA
    TGGAAGGAGT GCGTCGACAT CGCCACCAGC AGTCTTGGCA TTTCCGTGGG ATCGCTCTAC
    GTGGGCAAGC ACTTCCACAA GGATTCGAAG GCCAACGCCC TGGAGATGGT CAACGAAATC
    CGCAACGTGT TCAACGACAT CCTGGACGAG GTCAACTGGA TGGACGCCAA GACGAAGAAG
    GAGGCCAAGC TAAAGCTGCA CAGCATGGCC ACCCACATCG GCTATCCGGA CGAGATGCTC
    GACAACGAGA AGCTGGCCGC CTACTACGCC AAGCTCGACA TCGATCCCGA CAAGTACTTC
    GAGTCGTTCC TGGGCATGAA CATATTTGGC ACGGATTACT CGTTCAACAA GCTTCGTTTG
    CCGGTCAACA AGACGGACTG GGTTCGCCAC GCTCGTCCGG CGATCGTGAA CGCGTTCTAC
    TCGTCCTTAG AGAACAGTAT TCAATTCCCG GCCGGCATCC TGCAGGGGCA CTTCTTCAAC
    GCCCAACGTC CGAAGTACAT GAACTTTGGC GCCATTGGCT ACGTGATTGG TCACGAGATC
    ACCCATGGTT TCGACGACCA GGGTCGCCAG TTCGACGTGA AGGGCAACCT GCGGGACTGG
    TGGCACCCGG ACACCCAGAA GGCCTATCTG GCCAAGGCGA AGTGCATCAT TGAGCAGTAC
    GGCAACTACA CCGAAAGGGC CACGGGATTG AACTTGAATG GCATCAACAC CCAGGGGGAG
    AATATCGCCG ACAATGGTGG CGTCAAGGAG TCATACATCG CCTACCGCAG ATGGGCTGAG
    AAGCACGGAC CGGAAGCAAA GCTTCCGGGA TTGGACTACA CCCCGGAGCA GATGTTCTGG
    GTGGCAGCCG GACAGACTTG GTGTGCTAAG TACCGAAAAG AATCTCTCAA GATGCGTATT
    ACTACCGGCG TGCATTCTCC ATCCGAGTTC CGAGTCCTCG GGTCGCTCAG CAACATGAAG
    GACTTCGCCA AGGACTTCCA TTGTCCAGAG GGATCACCCA TGAATCCGGT CCAAAAGTGC
    GAGGTTTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_524227.1
    CDS317..2608
    Translation

    Target ORF information:

    RefSeq Version NM_079503.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neprilysin 2, transcript variant A (Nep2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_079503.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGACGG TAATCCAAAA CCCCAACTGG TGGCGTCGCC GTAACAAATT GGAAAAAAGT 
    CTGCTGGTCT CGCTGGGCAT CATGTTCGTG GTGCTTGCTA CCGGGTTCGG TCTCTGGATT
    GGCAAAGTGC TGAGGACGAG TCCCCCGTCG AATCCCCAGG CCACTGCTCT CCACGGCGAC
    AGCACCACCA TCAACCAGGT TCCAACGGGC ACCGCCTCGA AGGGCAAGTC GGGCGATTCG
    GGCGACGTGT GCCTCACCCA GGAGTGCATC CACACGGCTT CCACCGTTTT GCGGAAGATG
    AAGCCCGAGG TAGAGCCGTG CGACAACTTT TACGAGTTCG CCTGCGGCAC ATACCTTGAG
    GAGGAGAACA TCCCCGACGA CAAGGTCTCC ATCAGCACCT TCTCCGTGAT CTCCGACAAG
    CTGCAGGAGC AGTTGAAGGA CATCATCACC GCGGAGCGAC CGGAAACGGA GCCGAAGCAC
    TTCCGACTGC CCAACCTGCT TTACAAGGCC TGCATGAACA AAACTCTGAT CGAGACCCTG
    GGCCCTGAGC CCATAACTCG CGTGGCCGAG AGGCTGGGCG GCTGGCCGCT GATCAAGGGA
    GACTCTTGGA ACGCGGACGA CAGCTGGACT TGGCAGGAGC AGGTCAAGAA GTTCCGCACC
    GCCGGTTTTA GCATGGACTA CATCATCGAC TTTTCAATCG GAGTGGATTT GCAGAACAGC
    ACTAAGCGGC TTATAGATCT GGACCAGTCC TCCCTGGCCT TGAGTCGCGA GTACCTCGTG
    AAAGGATTCA ATGAGACGCT GGTGACGGCA TACTACAAGT ATATGGTTGA CATCGCGGTT
    CTTTTCGGAG CAAATAGGGA CTTGGCCAAG ACGGAGCTGC TGTTATCTTT GGAGTTCGAG
    ATGGCGCTGG CCAATATCTC CTGGCCCAAC GAGAAGCGAC GCAACTCCTC TGAGCTGTAC
    AATCTAAGAA CTCCAGCGCA GCTCCAGGCC GCCTACCCCT ACGTGCAGTG GGTGGACTAC
    ATGAACGCCC TGCTGCCGGA GGGACTGAAC GTGGCCGAGG ACGAGATGAT CAACCTGTCG
    GTGCCCAGCT TCTTCGAGGA CCTTGGCAAG CTGCTGGCCA AGACGCCGAA GCGTGTCATC
    GCCAACTATA TGTTCTGGCG TATCCACGGC TTCTCCGTGG GATTTCTGAG TGAGGAGTTC
    CGCAAGCGAC AGCTGCAGTA CGCCACAGCC CTTTCGGGGC GCCAGGAACA GGAGGCTAGA
    TGGAAGGAGT GCGTCGACAT CGCCACCAGC AGTCTTGGCA TTTCCGTGGG ATCGCTCTAC
    GTGGGCAAGC ACTTCCACAA GGATTCGAAG GCCAACGCCC TGGAGATGGT CAACGAAATC
    CGCAACGTGT TCAACGACAT CCTGGACGAG GTCAACTGGA TGGACGCCAA GACGAAGAAG
    GAGGCCAAGC TAAAGCTGCA CAGCATGGCC ACCCACATCG GCTATCCGGA CGAGATGCTC
    GACAACGAGA AGCTGGCCGC CTACTACGCC AAGCTCGACA TCGATCCCGA CAAGTACTTC
    GAGTCGTTCC TGGGCATGAA CATATTTGGC ACGGATTACT CGTTCAACAA GCTTCGTTTG
    CCGGTCAACA AGACGGACTG GGTTCGCCAC GCTCGTCCGG CGATCGTGAA CGCGTTCTAC
    TCGTCCTTAG AGAACAGTAT TCAATTCCCG GCCGGCATCC TGCAGGGGCA CTTCTTCAAC
    GCCCAACGTC CGAAGTACAT GAACTTTGGC GCCATTGGCT ACGTGATTGG TCACGAGATC
    ACCCATGGTT TCGACGACCA GGGTCGCCAG TTCGACGTGA AGGGCAACCT GCGGGACTGG
    TGGCACCCGG ACACCCAGAA GGCCTATCTG GCCAAGGCGA AGTGCATCAT TGAGCAGTAC
    GGCAACTACA CCGAAAGGGC CACGGGATTG AACTTGAATG GCATCAACAC CCAGGGGGAG
    AATATCGCCG ACAATGGTGG CGTCAAGGAG TCATACATCG CCTACCGCAG ATGGGCTGAG
    AAGCACGGAC CGGAAGCAAA GCTTCCGGGA TTGGACTACA CCCCGGAGCA GATGTTCTGG
    GTGGCAGCCG GACAGACTTG GTGTGCTAAG TACCGAAAAG AATCTCTCAA GATGCGTATT
    ACTACCGGCG TGCATTCTCC ATCCGAGTTC CGAGTCCTCG GGTCGCTCAG CAACATGAAG
    GACTTCGCCA AGGACTTCCA TTGTCCAGAG GGATCACCCA TGAATCCGGT CCAAAAGTGC
    GAGGTTTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08059
    Clone ID Related Accession (Same CDS sequence) NM_001259985.2
    Accession Version NM_001259985.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neprilysin 2, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001259985.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGACGG TAATCCAAAA CCCCAACTGG TGGCGTCGCC GTAACAAATT GGAAAAAAGT 
    CTGCTGGTCT CGCTGGGCAT CATGTTCGTG GTGCTTGCTA CCGGGTTCGG TCTCTGGATT
    GGCAAAGTGC TGAGGACGAG TCCCCCGTCG AATCCCCAGG CCACTGCTCT CCACGGCGAC
    AGCACCACCA TCAACCAGGT TCCAACGGGC ACCGCCTCGA AGGGCAAGTC GGGCGATTCG
    GGCGACGTGT GCCTCACCCA GGAGTGCATC CACACGGCTT CCACCGTTTT GCGGAAGATG
    AAGCCCGAGG TAGAGCCGTG CGACAACTTT TACGAGTTCG CCTGCGGCAC ATACCTTGAG
    GAGGAGAACA TCCCCGACGA CAAGGTCTCC ATCAGCACCT TCTCCGTGAT CTCCGACAAG
    CTGCAGGAGC AGTTGAAGGA CATCATCACC GCGGAGCGAC CGGAAACGGA GCCGAAGCAC
    TTCCGACTGC CCAACCTGCT TTACAAGGCC TGCATGAACA AAACTCTGAT CGAGACCCTG
    GGCCCTGAGC CCATAACTCG CGTGGCCGAG AGGCTGGGCG GCTGGCCGCT GATCAAGGGA
    GACTCTTGGA ACGCGGACGA CAGCTGGACT TGGCAGGAGC AGGTCAAGAA GTTCCGCACC
    GCCGGTTTTA GCATGGACTA CATCATCGAC TTTTCAATCG GAGTGGATTT GCAGAACAGC
    ACTAAGCGGC TTATAGATCT GGACCAGTCC TCCCTGGCCT TGAGTCGCGA GTACCTCGTG
    AAAGGATTCA ATGAGACGCT GGTGACGGCA TACTACAAGT ATATGGTTGA CATCGCGGTT
    CTTTTCGGAG CAAATAGGGA CTTGGCCAAG ACGGAGCTGC TGTTATCTTT GGAGTTCGAG
    ATGGCGCTGG CCAATATCTC CTGGCCCAAC GAGAAGCGAC GCAACTCCTC TGAGCTGTAC
    AATCTAAGAA CTCCAGCGCA GCTCCAGGCC GCCTACCCCT ACGTGCAGTG GGTGGACTAC
    ATGAACGCCC TGCTGCCGGA GGGACTGAAC GTGGCCGAGG ACGAGATGAT CAACCTGTCG
    GTGCCCAGCT TCTTCGAGGA CCTTGGCAAG CTGCTGGCCA AGACGCCGAA GCGTGTCATC
    GCCAACTATA TGTTCTGGCG TATCCACGGC TTCTCCGTGG GATTTCTGAG TGAGGAGTTC
    CGCAAGCGAC AGCTGCAGTA CGCCACAGCC CTTTCGGGGC GCCAGGAACA GGAGGCTAGA
    TGGAAGGAGT GCGTCGACAT CGCCACCAGC AGCATGGATG AAGTATGCGA AGATGATTTC
    GATAGTCTTG GCATTTCCGT GGGATCGCTC TACGTGGGCA AGCACTTCCA CAAGGATTCG
    AAGGCCAACG CCCTGGAGAT GGTCAACGAA ATCCGCAACG TGTTCAACGA CATCCTGGAC
    GAGGTCAACT GGATGGACGC CAAGACGAAG AAGGAGGCCA AGCTAAAGCT GCACAGCATG
    GCCACCCACA TCGGCTATCC GGACGAGATG CTCGACAACG AGAAGCTGGC CGCCTACTAC
    GCCAAGCTCG ACATCGATCC CGACAAGTAC TTCGAGTCGT TCCTGGGCAT GAACATATTT
    GGCACGGATT ACTCGTTCAA CAAGCTTCGT TTGCCGGTCA ACAAGACGGA CTGGGTTCGC
    CACGCTCGTC CGGCGATCGT GAACGCGTTC TACTCGTCCT TAGAGAACAG TATTCAATTC
    CCGGCCGGCA TCCTGCAGGG GCACTTCTTC AACGCCCAAC GTCCGAAGTA CATGAACTTT
    GGCGCCATTG GCTACGTGAT TGGTCACGAG ATCACCCATG GTTTCGACGA CCAGGGTCGC
    CAGTTCGACG TGAAGGGCAA CCTGCGGGAC TGGTGGCACC CGGACACCCA GAAGGCCTAT
    CTGGCCAAGG CGAAGTGCAT CATTGAGCAG TACGGCAACT ACACCGAAAG GGCCACGGGA
    TTGAACTTGA ATGGCATCAA CACCCAGGGG GAGAATATCG CCGACAATGG TGGCGTCAAG
    GAGTCATACA TCGCCTACCG CAGATGGGCT GAGAAGCACG GACCGGAAGC AAAGCTTCCG
    GGATTGGACT ACACCCCGGA GCAGATGTTC TGGGTGGCAG CCGGACAGAC TTGGTGTGCT
    AAGTACCGAA AAGAATCTCT CAAGATGCGT ATTACTACCG GCGTGCATTC TCCATCCGAG
    TTCCGAGTCC TCGGGTCGCT CAGCAACATG AAGGACTTCG CCAAGGACTT CCATTGTCCA
    GAGGGATCAC CCATGAATCC GGTCCAAAAG TGCGAGGTTT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246914.1
    CDS317..2641
    Translation

    Target ORF information:

    RefSeq Version NM_001259985.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neprilysin 2, transcript variant B (Nep2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259985.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGACGG TAATCCAAAA CCCCAACTGG TGGCGTCGCC GTAACAAATT GGAAAAAAGT 
    CTGCTGGTCT CGCTGGGCAT CATGTTCGTG GTGCTTGCTA CCGGGTTCGG TCTCTGGATT
    GGCAAAGTGC TGAGGACGAG TCCCCCGTCG AATCCCCAGG CCACTGCTCT CCACGGCGAC
    AGCACCACCA TCAACCAGGT TCCAACGGGC ACCGCCTCGA AGGGCAAGTC GGGCGATTCG
    GGCGACGTGT GCCTCACCCA GGAGTGCATC CACACGGCTT CCACCGTTTT GCGGAAGATG
    AAGCCCGAGG TAGAGCCGTG CGACAACTTT TACGAGTTCG CCTGCGGCAC ATACCTTGAG
    GAGGAGAACA TCCCCGACGA CAAGGTCTCC ATCAGCACCT TCTCCGTGAT CTCCGACAAG
    CTGCAGGAGC AGTTGAAGGA CATCATCACC GCGGAGCGAC CGGAAACGGA GCCGAAGCAC
    TTCCGACTGC CCAACCTGCT TTACAAGGCC TGCATGAACA AAACTCTGAT CGAGACCCTG
    GGCCCTGAGC CCATAACTCG CGTGGCCGAG AGGCTGGGCG GCTGGCCGCT GATCAAGGGA
    GACTCTTGGA ACGCGGACGA CAGCTGGACT TGGCAGGAGC AGGTCAAGAA GTTCCGCACC
    GCCGGTTTTA GCATGGACTA CATCATCGAC TTTTCAATCG GAGTGGATTT GCAGAACAGC
    ACTAAGCGGC TTATAGATCT GGACCAGTCC TCCCTGGCCT TGAGTCGCGA GTACCTCGTG
    AAAGGATTCA ATGAGACGCT GGTGACGGCA TACTACAAGT ATATGGTTGA CATCGCGGTT
    CTTTTCGGAG CAAATAGGGA CTTGGCCAAG ACGGAGCTGC TGTTATCTTT GGAGTTCGAG
    ATGGCGCTGG CCAATATCTC CTGGCCCAAC GAGAAGCGAC GCAACTCCTC TGAGCTGTAC
    AATCTAAGAA CTCCAGCGCA GCTCCAGGCC GCCTACCCCT ACGTGCAGTG GGTGGACTAC
    ATGAACGCCC TGCTGCCGGA GGGACTGAAC GTGGCCGAGG ACGAGATGAT CAACCTGTCG
    GTGCCCAGCT TCTTCGAGGA CCTTGGCAAG CTGCTGGCCA AGACGCCGAA GCGTGTCATC
    GCCAACTATA TGTTCTGGCG TATCCACGGC TTCTCCGTGG GATTTCTGAG TGAGGAGTTC
    CGCAAGCGAC AGCTGCAGTA CGCCACAGCC CTTTCGGGGC GCCAGGAACA GGAGGCTAGA
    TGGAAGGAGT GCGTCGACAT CGCCACCAGC AGCATGGATG AAGTATGCGA AGATGATTTC
    GATAGTCTTG GCATTTCCGT GGGATCGCTC TACGTGGGCA AGCACTTCCA CAAGGATTCG
    AAGGCCAACG CCCTGGAGAT GGTCAACGAA ATCCGCAACG TGTTCAACGA CATCCTGGAC
    GAGGTCAACT GGATGGACGC CAAGACGAAG AAGGAGGCCA AGCTAAAGCT GCACAGCATG
    GCCACCCACA TCGGCTATCC GGACGAGATG CTCGACAACG AGAAGCTGGC CGCCTACTAC
    GCCAAGCTCG ACATCGATCC CGACAAGTAC TTCGAGTCGT TCCTGGGCAT GAACATATTT
    GGCACGGATT ACTCGTTCAA CAAGCTTCGT TTGCCGGTCA ACAAGACGGA CTGGGTTCGC
    CACGCTCGTC CGGCGATCGT GAACGCGTTC TACTCGTCCT TAGAGAACAG TATTCAATTC
    CCGGCCGGCA TCCTGCAGGG GCACTTCTTC AACGCCCAAC GTCCGAAGTA CATGAACTTT
    GGCGCCATTG GCTACGTGAT TGGTCACGAG ATCACCCATG GTTTCGACGA CCAGGGTCGC
    CAGTTCGACG TGAAGGGCAA CCTGCGGGAC TGGTGGCACC CGGACACCCA GAAGGCCTAT
    CTGGCCAAGG CGAAGTGCAT CATTGAGCAG TACGGCAACT ACACCGAAAG GGCCACGGGA
    TTGAACTTGA ATGGCATCAA CACCCAGGGG GAGAATATCG CCGACAATGG TGGCGTCAAG
    GAGTCATACA TCGCCTACCG CAGATGGGCT GAGAAGCACG GACCGGAAGC AAAGCTTCCG
    GGATTGGACT ACACCCCGGA GCAGATGTTC TGGGTGGCAG CCGGACAGAC TTGGTGTGCT
    AAGTACCGAA AAGAATCTCT CAAGATGCGT ATTACTACCG GCGTGCATTC TCCATCCGAG
    TTCCGAGTCC TCGGGTCGCT CAGCAACATG AAGGACTTCG CCAAGGACTT CCATTGTCCA
    GAGGGATCAC CCATGAATCC GGTCCAAAAG TGCGAGGTTT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.