eIF3a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIF3a
Entrez Gene ID	40563
Full Name	CG9805 gene product from transcript CG9805-RD
Synonyms	CG9805,Dmel\CG9805,EIF3-S10,MMAP140,anon-WO0118547.261,eIF3-s10,eIF3a
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 82B1-82B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_168999.3	NP_730838.3	eukaryotic translation initiation factor 3 subunit a, isoform D
NM_141213.4	NP_649470.2	eukaryotic translation initiation factor 3 subunit a, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	EIF3A	NP_003741.1
Pan troglodytes (chimpanzee)	EIF3A	XP_001153575.1
Mus musculus (house mouse)	Eif3a	NP_034253.3
Oryza sativa (rice)	Os01g0120800	NP_001041871.1
Danio rerio (zebrafish)	eif3s10	NP_956114.2
Anopheles gambiae (African malaria mosquito)	EIF3A_ANOGA	XP_312628.5
Caenorhabditis elegans (roundworm)	egl-45	NP_498698.1
Saccharomyces cerevisiae (baker's yeast)	RPG1	NP_009635.1
Arabidopsis thaliana (thale cress)	EIF3A	NP_192881.1
Bos taurus (cattle)	EIF3A	NP_001193243.1
Drosophila melanogaster (fruit fly)	eIF3-S10	NP_649470.2
Kluyveromyces lactis	KLLA0C14498g	XP_452849.1
Neurospora crassa	NCU00040	XP_956151.2
Rattus norvegicus (Norway rat)	Eif3a	NP_001040552.2
Gallus gallus (chicken)	EIF3A	XP_421787.3
Schizosaccharomyces pombe (fission yeast)	tif32	NP_596379.1
Magnaporthe oryzae (rice blast fungus)	MGG_10192	XP_003713822.1
Canis lupus familiaris (dog)	EIF3A	XP_852131.1
Eremothecium gossypii	AGOS_ABL065W	NP_982882.1
Xenopus tropicalis (tropical clawed frog)	eif3a	NP_001096173.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eIF3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03830	NM_141213.4 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 3 subunit a (eIF3a), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa03830	NM_168999.3 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 3 subunit a (eIF3a), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIF3a ( NM_168999.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730838.3 Drosophila melanogaster eukaryotic translation initiation factor 3 subunit a (eIF3a), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa03830
Clone ID Related Accession (Same CDS sequence)	NM_168999.3 , NM_141213.4
Accession Version	NM_168999.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 3 subunit a, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_168999.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCGCT ATACGCAACG TCCGGAAAAC GCCCTAAAAA GGGCTAATGA GTTCATTGAA 
GTGGGAAAGC CGCTTAGGGC GCTGGACACG CTGCAGGAAG TTTTCCGGAA CAAGCGATGG 
AACTATGCCT ACTCGGAGAC GGTCATCGAA CCGCTCATGT TCAAGTACCT GTACCTCTGC 
GTGGAACTCA AGAAGTCGCA CATCGCCAAG GAGGGACTAT TCCAGTACCG GAACATGTTC 
CAGCTGGTGA ACGTGAACTC TCTGGAAAAT GTAATCCGCG GCTACCTGAA AATGGCCGAA 
GAGCACACAG AGGCTGCCCA AGCCCAGTCC TCGGCGGCCG TTGCTGTTCT GGAGCTGGAT 
GACCTGGACA ACATTGCCAC GCCAGAGAGC ATCCTAATGA GCGCAGTGTG TGGCGAGGAC 
GCACAGGACC GCTCCGACCG CACTATTCTG CTCCCGTGGG TCAAGTTCCT GTGGGAGTCC 
TACTGCCAGT GTCTAGAGCT GCTGCGCGTA AACACGCATT GCGAGGCGCT GTACCACGAT 
ATCGCTCGCA TGGCCTTCCA GTTCTGCCTT AAGTACAACC GCAAGAGCGA GTTTCGCCGC 
TTGTGCGACA AGCTGCGCAA ACACTTGGAG GACATTTGCA AGAGCAGCAA CCAGACCACC 
GGTGTCTCGA TCAACAAGGT GGAAACGCAG CAGCTCTGCT TGGACACCAG GTTGTACCTT 
CTTGACTCGG CCATCCAGAT GGAGCTGTGG CAAGAGGCCT ACAAGGCAAT CGAGGATATC 
CATGGCCTGA TGGCTCTGTC CAAGAAGACA CCCGTGCCCA AAACCATGGC CAACTACTAC 
CAAAAATTAG CCATGGTCTT TAGCAAGGCC GGCAACCAGT TGTTCCACGC TGCAGCGCTG 
CTGAAGCTCT TCCAGCTGAC GCGCGAGCTG AAGAAGAACC TGACGAAGGA CGACTTGCAG 
CGTATGGCTG CCCACGTTCT CCTGGCCACT CTTTCTATTC CGTTGCCTTC GGCCCATCCA 
GAGTTCGACA GGTTTATTGA GGCGGACAAG AGCCCCCTGG AGAAGGCGCA GAAGTTGGCC 
GTGCTTTTGG GCCTGCCCCA GCCACCTACT CGCGTCTCCC TTATTCGTGA AGTGGTGCGC 
TTGAATGTTC CTCAACTCGT ATCTGAGGAC TTTCGCAATC TATACAACTG GTTGGAGGTG 
GATTTCAACC CACTGAATCT GTGCAAGCGC ATTCAGTCTA TCGTGGACTT CATTGAGAAC 
GGGCCCGAAA ACGCTCTGCT CACTCCTTAC ATTCAATCGC TGAAGGACGT GACTATAATG 
CGTCTGATTC GTCAGATCTC TCAGGTTTAT GAGAGTATTA AGTTCCAGCG TCTCTTGCAG 
CTGGCCAGTT TTTGCAACAT TTTTGAATTG GAAAAACTGC TGGTCGAGTC CGTGCGTCAC 
AATGACATGC AGATTCGTAT TGACCACCAG AAGAACAGTA TCTATTTCGG CACTGATCTG 
ACCGAGAGTC AACGCGAGTA CCGCCCCGAC GGACCCGCTT TGCAATCGAT GCCCTCGGAA 
CAGATCCGCT CTCAGTTGGT TAATATGTCT ACTGTTCTGA CACGGGCAGT TTCAATCGTC 
TACCCGAACC GTGAACGTGA CCAGCGGGCC AAGCTCCGCA ACCAGATGGT AAACCATTAC 
CATGAGATCA AGGATCGCGA GCACCAGCGT ATTCTTCAGC GACAGAAGAT CATCGAGGAC 
AGAAAGGAGT ACATCGAGAA GCAGAATAAT GCTCGTGAAG AGGAAGAGGC GCGGCGTCAG 
GAGGAGGAAT CGCGGAAGGC CAAACTGGCC GAACAGAAGC GCCTCGAGCA GGAGCAAGAA 
GAAAGAGAGC GCAAGCGCCA TCAGAACGAA ATCCAAGCCA TCCGGGAGAA GAGTCTCAAG 
GAGAAGGTTC AGCAAATCTC GCAGACGGCT CATGGTAAGA AGATGCTTTC CAAGCTGGAT 
GAGGAAGGCA TTAAGAAGTT GGACGCCGAG CAGATCGCCA AGCGGGAAAG CGAGGAACTT 
CAGCGCGAGG CCAAGGAACT GCAGTCGAAA CTCAAATCGC AAGAGAAGAA AATCGACTAC 
TTCGAACGCG CCAAGCGCCT GGAGGAGATA CCGCTTTTCG AAAAATACTT GGCCGAAAAG 
CAGGTTAAGG ACAAGGAGTT CTGGGAAGCA ACCGAGAAGA CACGCATCGA GAATGCCATT 
GCCGAACGGA AGGATGCCGT CGCCCAGCAG GAGCGTCTCA AGCGCATGTA TCCGGACCGG 
GATGAATTCC TTGAGGCCCT TAAAAAAGAA CGTGCCTCCC TCTACGTCGA AAAGCTCAAA 
AAGTTTGAGG CTGCTCTGGA GGCAGAGCGC AAAAAGCGCT TAGCCGACCG CATTATTCGA 
CGCCGCGAGG AGCGCCGACA GGCCTTCCTT CGCGAGAAAG AGGAGGAGCG TCTTCGCAAG 
GAGGAAGAGA TTCGCTTGGC ACAGGCTGCC GAAGAGCGCG CCGCAGCAGA AGCCCGCCGT 
TTGGAGCGTG AGGCTGAGGA CGAAAAGCGT CGTGCCCAAT ACGAAAAGCA GCGTGCCAAG 
GAGGAGGAAG CTGAGCGTAA AATCAAGGAA GATCGAGACC GCCTGTCCAG GGAGTTGGCC 
TCGGAGCGCG AACGCACTGA AAAGGACCGT GACACATGGC GTCCTCGAGG CGGTGACCGT 
CCAAGCGCAT CTAACGGCGG ATCCAGCGAG TGGCGTCGTG CTGCACCTGC GGCCAGTGAA 
CGCAACGACC GTGGTGGCGA GCGTATCGAG CGCGGAGGAG AACGGATCGA GCGCGGTGGA 
GAACGCCTCG AGCGTGGTGG AGAGCGCATT GAACGAGGCG GTGACCGTGA CCGCAAGGAC 
AACGAGGGAG CGGACTCCTC TTGGCGTGTG CGACGAGAGC CAGATTCACA GCGTGCAGCT 
GCTCCCAAAG ACAGTGGCGC TCCGCAATCG CGCGATGACA AGTGGCGGCG TGGCGGAGAA 
CGTGACCGCG ATTTCCGCAT TGATGGGGCT CGTCGCGACA GGGATGATGG GCCTCGGCGT 
GACCGTGACG ATGGTCCTCG CCGGGATCGT GACGATGAGC GCGGTGGATT CCGCCGCGCC 
GACGGGGCGC GTCGTACCGA CGAGCCTCAG CGGGAAACTG GAGGGAACTG GCGCGACGCT 
CCACGCCACG CTGATCGGGA AACCCGTCGC CCAGCTGAGA GGCGCGACCG AGATGTGCGC 
GAGACTCGTG GCGATCAACG CGGCTCTGCT CCTAAGGAGG CAGCCAGTGG CGGAGTAGGC 
GGAAACTGGC GTACTGCTCC TGCGACCCGT GAGGAAAAAC CAGCAGCTAA GCGCGATCAA 
GCCCAGGAAA AGGAGAACAA AGCTGGCGAT GATGGCGAGT GGACCAGCGT TAAGCGTCGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730838.3
CDS	149..3571
Translation	MARYTQRPENALKRANEFIEVGKPLRALDTLQEVFRNKRWNYAYSETVIEPLMFKYLYLCVELKKSHIAKEGLFQYRNMFQLVNVNSLENVIRGYLKMAEEHTEAAQAQSSAAVAVLELDDLDNIATPESILMSAVCGEDAQDRSDRTILLPWVKFLWESYCQCLELLRVNTHCEALYHDIARMAFQFCLKYNRKSEFRRLCDKLRKHLEDICKSSNQTTGVSINKVETQQLCLDTRLYLLDSAIQMELWQEAYKAIEDIHGLMALSKKTPVPKTMANYYQKLAMVFSKAGNQLFHAAALLKLFQLTRELKKNLTKDDLQRMAAHVLLATLSIPLPSAHPEFDRFIEADKSPLEKAQKLAVLLGLPQPPTRVSLIREVVRLNVPQLVSEDFRNLYNWLEVDFNPLNLCKRIQSIVDFIENGPENALLTPYIQSLKDVTIMRLIRQISQVYESIKFQRLLQLASFCNIFELEKLLVESVRHNDMQIRIDHQKNSIYFGTDLTESQREYRPDGPALQSMPSEQIRSQLVNMSTVLTRAVSIVYPNRERDQRAKLRNQMVNHYHEIKDREHQRILQRQKIIEDRKEYIEKQNNAREEEEARRQEEESRKAKLAEQKRLEQEQEERERKRHQNEIQAIREKSLKEKVQQISQTAHGKKMLSKLDEEGIKKLDAEQIAKRESEELQREAKELQSKLKSQEKKIDYFERAKRLEEIPLFEKYLAEKQVKDKEFWEATEKTRIENAIAERKDAVAQQERLKRMYPDRDEFLEALKKERASLYVEKLKKFEAALEAERKKRLADRIIRRREERRQAFLREKEEERLRKEEEIRLAQAAEERAAAEARRLEREAEDEKRRAQYEKQRAKEEEAERKIKEDRDRLSRELASERERTEKDRDTWRPRGGDRPSASNGGSSEWRRAAPAASERNDRGGERIERGGERIERGGERLERGGERIERGGDRDRKDNEGADSSWRVRREPDSQRAAAPKDSGAPQSRDDKWRRGGERDRDFRIDGARRDRDDGPRRDRDDGPRRDRDDERGGFRRADGARRTDEPQRETGGNWRDAPRHADRETRRPAERRDRDVRETRGDQRGSAPKEAASGGVGGNWRTAPATREEKPAAKRDQAQEKENKAGDDGEWTSVKRR

Target ORF information:

RefSeq Version	NM_168999.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 3 subunit a (eIF3a), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168999.3

ORF Insert Sequence:

ATGGCCCGCT ATACGCAACG TCCGGAAAAC GCCCTAAAAA GGGCTAATGA GTTCATTGAA 
GTGGGAAAGC CGCTTAGGGC GCTGGACACG CTGCAGGAAG TTTTCCGGAA CAAGCGATGG 
AACTATGCCT ACTCGGAGAC GGTCATCGAA CCGCTCATGT TCAAGTACCT GTACCTCTGC 
GTGGAACTCA AGAAGTCGCA CATCGCCAAG GAGGGACTAT TCCAGTACCG GAACATGTTC 
CAGCTGGTGA ACGTGAACTC TCTGGAAAAT GTAATCCGCG GCTACCTGAA AATGGCCGAA 
GAGCACACAG AGGCTGCCCA AGCCCAGTCC TCGGCGGCCG TTGCTGTTCT GGAGCTGGAT 
GACCTGGACA ACATTGCCAC GCCAGAGAGC ATCCTAATGA GCGCAGTGTG TGGCGAGGAC 
GCACAGGACC GCTCCGACCG CACTATTCTG CTCCCGTGGG TCAAGTTCCT GTGGGAGTCC 
TACTGCCAGT GTCTAGAGCT GCTGCGCGTA AACACGCATT GCGAGGCGCT GTACCACGAT 
ATCGCTCGCA TGGCCTTCCA GTTCTGCCTT AAGTACAACC GCAAGAGCGA GTTTCGCCGC 
TTGTGCGACA AGCTGCGCAA ACACTTGGAG GACATTTGCA AGAGCAGCAA CCAGACCACC 
GGTGTCTCGA TCAACAAGGT GGAAACGCAG CAGCTCTGCT TGGACACCAG GTTGTACCTT 
CTTGACTCGG CCATCCAGAT GGAGCTGTGG CAAGAGGCCT ACAAGGCAAT CGAGGATATC 
CATGGCCTGA TGGCTCTGTC CAAGAAGACA CCCGTGCCCA AAACCATGGC CAACTACTAC 
CAAAAATTAG CCATGGTCTT TAGCAAGGCC GGCAACCAGT TGTTCCACGC TGCAGCGCTG 
CTGAAGCTCT TCCAGCTGAC GCGCGAGCTG AAGAAGAACC TGACGAAGGA CGACTTGCAG 
CGTATGGCTG CCCACGTTCT CCTGGCCACT CTTTCTATTC CGTTGCCTTC GGCCCATCCA 
GAGTTCGACA GGTTTATTGA GGCGGACAAG AGCCCCCTGG AGAAGGCGCA GAAGTTGGCC 
GTGCTTTTGG GCCTGCCCCA GCCACCTACT CGCGTCTCCC TTATTCGTGA AGTGGTGCGC 
TTGAATGTTC CTCAACTCGT ATCTGAGGAC TTTCGCAATC TATACAACTG GTTGGAGGTG 
GATTTCAACC CACTGAATCT GTGCAAGCGC ATTCAGTCTA TCGTGGACTT CATTGAGAAC 
GGGCCCGAAA ACGCTCTGCT CACTCCTTAC ATTCAATCGC TGAAGGACGT GACTATAATG 
CGTCTGATTC GTCAGATCTC TCAGGTTTAT GAGAGTATTA AGTTCCAGCG TCTCTTGCAG 
CTGGCCAGTT TTTGCAACAT TTTTGAATTG GAAAAACTGC TGGTCGAGTC CGTGCGTCAC 
AATGACATGC AGATTCGTAT TGACCACCAG AAGAACAGTA TCTATTTCGG CACTGATCTG 
ACCGAGAGTC AACGCGAGTA CCGCCCCGAC GGACCCGCTT TGCAATCGAT GCCCTCGGAA 
CAGATCCGCT CTCAGTTGGT TAATATGTCT ACTGTTCTGA CACGGGCAGT TTCAATCGTC 
TACCCGAACC GTGAACGTGA CCAGCGGGCC AAGCTCCGCA ACCAGATGGT AAACCATTAC 
CATGAGATCA AGGATCGCGA GCACCAGCGT ATTCTTCAGC GACAGAAGAT CATCGAGGAC 
AGAAAGGAGT ACATCGAGAA GCAGAATAAT GCTCGTGAAG AGGAAGAGGC GCGGCGTCAG 
GAGGAGGAAT CGCGGAAGGC CAAACTGGCC GAACAGAAGC GCCTCGAGCA GGAGCAAGAA 
GAAAGAGAGC GCAAGCGCCA TCAGAACGAA ATCCAAGCCA TCCGGGAGAA GAGTCTCAAG 
GAGAAGGTTC AGCAAATCTC GCAGACGGCT CATGGTAAGA AGATGCTTTC CAAGCTGGAT 
GAGGAAGGCA TTAAGAAGTT GGACGCCGAG CAGATCGCCA AGCGGGAAAG CGAGGAACTT 
CAGCGCGAGG CCAAGGAACT GCAGTCGAAA CTCAAATCGC AAGAGAAGAA AATCGACTAC 
TTCGAACGCG CCAAGCGCCT GGAGGAGATA CCGCTTTTCG AAAAATACTT GGCCGAAAAG 
CAGGTTAAGG ACAAGGAGTT CTGGGAAGCA ACCGAGAAGA CACGCATCGA GAATGCCATT 
GCCGAACGGA AGGATGCCGT CGCCCAGCAG GAGCGTCTCA AGCGCATGTA TCCGGACCGG 
GATGAATTCC TTGAGGCCCT TAAAAAAGAA CGTGCCTCCC TCTACGTCGA AAAGCTCAAA 
AAGTTTGAGG CTGCTCTGGA GGCAGAGCGC AAAAAGCGCT TAGCCGACCG CATTATTCGA 
CGCCGCGAGG AGCGCCGACA GGCCTTCCTT CGCGAGAAAG AGGAGGAGCG TCTTCGCAAG 
GAGGAAGAGA TTCGCTTGGC ACAGGCTGCC GAAGAGCGCG CCGCAGCAGA AGCCCGCCGT 
TTGGAGCGTG AGGCTGAGGA CGAAAAGCGT CGTGCCCAAT ACGAAAAGCA GCGTGCCAAG 
GAGGAGGAAG CTGAGCGTAA AATCAAGGAA GATCGAGACC GCCTGTCCAG GGAGTTGGCC 
TCGGAGCGCG AACGCACTGA AAAGGACCGT GACACATGGC GTCCTCGAGG CGGTGACCGT 
CCAAGCGCAT CTAACGGCGG ATCCAGCGAG TGGCGTCGTG CTGCACCTGC GGCCAGTGAA 
CGCAACGACC GTGGTGGCGA GCGTATCGAG CGCGGAGGAG AACGGATCGA GCGCGGTGGA 
GAACGCCTCG AGCGTGGTGG AGAGCGCATT GAACGAGGCG GTGACCGTGA CCGCAAGGAC 
AACGAGGGAG CGGACTCCTC TTGGCGTGTG CGACGAGAGC CAGATTCACA GCGTGCAGCT 
GCTCCCAAAG ACAGTGGCGC TCCGCAATCG CGCGATGACA AGTGGCGGCG TGGCGGAGAA 
CGTGACCGCG ATTTCCGCAT TGATGGGGCT CGTCGCGACA GGGATGATGG GCCTCGGCGT 
GACCGTGACG ATGGTCCTCG CCGGGATCGT GACGATGAGC GCGGTGGATT CCGCCGCGCC 
GACGGGGCGC GTCGTACCGA CGAGCCTCAG CGGGAAACTG GAGGGAACTG GCGCGACGCT 
CCACGCCACG CTGATCGGGA AACCCGTCGC CCAGCTGAGA GGCGCGACCG AGATGTGCGC 
GAGACTCGTG GCGATCAACG CGGCTCTGCT CCTAAGGAGG CAGCCAGTGG CGGAGTAGGC 
GGAAACTGGC GTACTGCTCC TGCGACCCGT GAGGAAAAAC CAGCAGCTAA GCGCGATCAA 
GCCCAGGAAA AGGAGAACAA AGCTGGCGAT GATGGCGAGT GGACCAGCGT TAAGCGTCGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF3a ( NM_141213.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649470.2 Drosophila melanogaster eukaryotic translation initiation factor 3 subunit a (eIF3a), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03830
Clone ID Related Accession (Same CDS sequence)	NM_168999.3 , NM_141213.4
Accession Version	NM_141213.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 3 subunit a, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141213.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCGCT ATACGCAACG TCCGGAAAAC GCCCTAAAAA GGGCTAATGA GTTCATTGAA 
GTGGGAAAGC CGCTTAGGGC GCTGGACACG CTGCAGGAAG TTTTCCGGAA CAAGCGATGG 
AACTATGCCT ACTCGGAGAC GGTCATCGAA CCGCTCATGT TCAAGTACCT GTACCTCTGC 
GTGGAACTCA AGAAGTCGCA CATCGCCAAG GAGGGACTAT TCCAGTACCG GAACATGTTC 
CAGCTGGTGA ACGTGAACTC TCTGGAAAAT GTAATCCGCG GCTACCTGAA AATGGCCGAA 
GAGCACACAG AGGCTGCCCA AGCCCAGTCC TCGGCGGCCG TTGCTGTTCT GGAGCTGGAT 
GACCTGGACA ACATTGCCAC GCCAGAGAGC ATCCTAATGA GCGCAGTGTG TGGCGAGGAC 
GCACAGGACC GCTCCGACCG CACTATTCTG CTCCCGTGGG TCAAGTTCCT GTGGGAGTCC 
TACTGCCAGT GTCTAGAGCT GCTGCGCGTA AACACGCATT GCGAGGCGCT GTACCACGAT 
ATCGCTCGCA TGGCCTTCCA GTTCTGCCTT AAGTACAACC GCAAGAGCGA GTTTCGCCGC 
TTGTGCGACA AGCTGCGCAA ACACTTGGAG GACATTTGCA AGAGCAGCAA CCAGACCACC 
GGTGTCTCGA TCAACAAGGT GGAAACGCAG CAGCTCTGCT TGGACACCAG GTTGTACCTT 
CTTGACTCGG CCATCCAGAT GGAGCTGTGG CAAGAGGCCT ACAAGGCAAT CGAGGATATC 
CATGGCCTGA TGGCTCTGTC CAAGAAGACA CCCGTGCCCA AAACCATGGC CAACTACTAC 
CAAAAATTAG CCATGGTCTT TAGCAAGGCC GGCAACCAGT TGTTCCACGC TGCAGCGCTG 
CTGAAGCTCT TCCAGCTGAC GCGCGAGCTG AAGAAGAACC TGACGAAGGA CGACTTGCAG 
CGTATGGCTG CCCACGTTCT CCTGGCCACT CTTTCTATTC CGTTGCCTTC GGCCCATCCA 
GAGTTCGACA GGTTTATTGA GGCGGACAAG AGCCCCCTGG AGAAGGCGCA GAAGTTGGCC 
GTGCTTTTGG GCCTGCCCCA GCCACCTACT CGCGTCTCCC TTATTCGTGA AGTGGTGCGC 
TTGAATGTTC CTCAACTCGT ATCTGAGGAC TTTCGCAATC TATACAACTG GTTGGAGGTG 
GATTTCAACC CACTGAATCT GTGCAAGCGC ATTCAGTCTA TCGTGGACTT CATTGAGAAC 
GGGCCCGAAA ACGCTCTGCT CACTCCTTAC ATTCAATCGC TGAAGGACGT GACTATAATG 
CGTCTGATTC GTCAGATCTC TCAGGTTTAT GAGAGTATTA AGTTCCAGCG TCTCTTGCAG 
CTGGCCAGTT TTTGCAACAT TTTTGAATTG GAAAAACTGC TGGTCGAGTC CGTGCGTCAC 
AATGACATGC AGATTCGTAT TGACCACCAG AAGAACAGTA TCTATTTCGG CACTGATCTG 
ACCGAGAGTC AACGCGAGTA CCGCCCCGAC GGACCCGCTT TGCAATCGAT GCCCTCGGAA 
CAGATCCGCT CTCAGTTGGT TAATATGTCT ACTGTTCTGA CACGGGCAGT TTCAATCGTC 
TACCCGAACC GTGAACGTGA CCAGCGGGCC AAGCTCCGCA ACCAGATGGT AAACCATTAC 
CATGAGATCA AGGATCGCGA GCACCAGCGT ATTCTTCAGC GACAGAAGAT CATCGAGGAC 
AGAAAGGAGT ACATCGAGAA GCAGAATAAT GCTCGTGAAG AGGAAGAGGC GCGGCGTCAG 
GAGGAGGAAT CGCGGAAGGC CAAACTGGCC GAACAGAAGC GCCTCGAGCA GGAGCAAGAA 
GAAAGAGAGC GCAAGCGCCA TCAGAACGAA ATCCAAGCCA TCCGGGAGAA GAGTCTCAAG 
GAGAAGGTTC AGCAAATCTC GCAGACGGCT CATGGTAAGA AGATGCTTTC CAAGCTGGAT 
GAGGAAGGCA TTAAGAAGTT GGACGCCGAG CAGATCGCCA AGCGGGAAAG CGAGGAACTT 
CAGCGCGAGG CCAAGGAACT GCAGTCGAAA CTCAAATCGC AAGAGAAGAA AATCGACTAC 
TTCGAACGCG CCAAGCGCCT GGAGGAGATA CCGCTTTTCG AAAAATACTT GGCCGAAAAG 
CAGGTTAAGG ACAAGGAGTT CTGGGAAGCA ACCGAGAAGA CACGCATCGA GAATGCCATT 
GCCGAACGGA AGGATGCCGT CGCCCAGCAG GAGCGTCTCA AGCGCATGTA TCCGGACCGG 
GATGAATTCC TTGAGGCCCT TAAAAAAGAA CGTGCCTCCC TCTACGTCGA AAAGCTCAAA 
AAGTTTGAGG CTGCTCTGGA GGCAGAGCGC AAAAAGCGCT TAGCCGACCG CATTATTCGA 
CGCCGCGAGG AGCGCCGACA GGCCTTCCTT CGCGAGAAAG AGGAGGAGCG TCTTCGCAAG 
GAGGAAGAGA TTCGCTTGGC ACAGGCTGCC GAAGAGCGCG CCGCAGCAGA AGCCCGCCGT 
TTGGAGCGTG AGGCTGAGGA CGAAAAGCGT CGTGCCCAAT ACGAAAAGCA GCGTGCCAAG 
GAGGAGGAAG CTGAGCGTAA AATCAAGGAA GATCGAGACC GCCTGTCCAG GGAGTTGGCC 
TCGGAGCGCG AACGCACTGA AAAGGACCGT GACACATGGC GTCCTCGAGG CGGTGACCGT 
CCAAGCGCAT CTAACGGCGG ATCCAGCGAG TGGCGTCGTG CTGCACCTGC GGCCAGTGAA 
CGCAACGACC GTGGTGGCGA GCGTATCGAG CGCGGAGGAG AACGGATCGA GCGCGGTGGA 
GAACGCCTCG AGCGTGGTGG AGAGCGCATT GAACGAGGCG GTGACCGTGA CCGCAAGGAC 
AACGAGGGAG CGGACTCCTC TTGGCGTGTG CGACGAGAGC CAGATTCACA GCGTGCAGCT 
GCTCCCAAAG ACAGTGGCGC TCCGCAATCG CGCGATGACA AGTGGCGGCG TGGCGGAGAA 
CGTGACCGCG ATTTCCGCAT TGATGGGGCT CGTCGCGACA GGGATGATGG GCCTCGGCGT 
GACCGTGACG ATGGTCCTCG CCGGGATCGT GACGATGAGC GCGGTGGATT CCGCCGCGCC 
GACGGGGCGC GTCGTACCGA CGAGCCTCAG CGGGAAACTG GAGGGAACTG GCGCGACGCT 
CCACGCCACG CTGATCGGGA AACCCGTCGC CCAGCTGAGA GGCGCGACCG AGATGTGCGC 
GAGACTCGTG GCGATCAACG CGGCTCTGCT CCTAAGGAGG CAGCCAGTGG CGGAGTAGGC 
GGAAACTGGC GTACTGCTCC TGCGACCCGT GAGGAAAAAC CAGCAGCTAA GCGCGATCAA 
GCCCAGGAAA AGGAGAACAA AGCTGGCGAT GATGGCGAGT GGACCAGCGT TAAGCGTCGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649470.2
CDS	149..3571
Translation	MARYTQRPENALKRANEFIEVGKPLRALDTLQEVFRNKRWNYAYSETVIEPLMFKYLYLCVELKKSHIAKEGLFQYRNMFQLVNVNSLENVIRGYLKMAEEHTEAAQAQSSAAVAVLELDDLDNIATPESILMSAVCGEDAQDRSDRTILLPWVKFLWESYCQCLELLRVNTHCEALYHDIARMAFQFCLKYNRKSEFRRLCDKLRKHLEDICKSSNQTTGVSINKVETQQLCLDTRLYLLDSAIQMELWQEAYKAIEDIHGLMALSKKTPVPKTMANYYQKLAMVFSKAGNQLFHAAALLKLFQLTRELKKNLTKDDLQRMAAHVLLATLSIPLPSAHPEFDRFIEADKSPLEKAQKLAVLLGLPQPPTRVSLIREVVRLNVPQLVSEDFRNLYNWLEVDFNPLNLCKRIQSIVDFIENGPENALLTPYIQSLKDVTIMRLIRQISQVYESIKFQRLLQLASFCNIFELEKLLVESVRHNDMQIRIDHQKNSIYFGTDLTESQREYRPDGPALQSMPSEQIRSQLVNMSTVLTRAVSIVYPNRERDQRAKLRNQMVNHYHEIKDREHQRILQRQKIIEDRKEYIEKQNNAREEEEARRQEEESRKAKLAEQKRLEQEQEERERKRHQNEIQAIREKSLKEKVQQISQTAHGKKMLSKLDEEGIKKLDAEQIAKRESEELQREAKELQSKLKSQEKKIDYFERAKRLEEIPLFEKYLAEKQVKDKEFWEATEKTRIENAIAERKDAVAQQERLKRMYPDRDEFLEALKKERASLYVEKLKKFEAALEAERKKRLADRIIRRREERRQAFLREKEEERLRKEEEIRLAQAAEERAAAEARRLEREAEDEKRRAQYEKQRAKEEEAERKIKEDRDRLSRELASERERTEKDRDTWRPRGGDRPSASNGGSSEWRRAAPAASERNDRGGERIERGGERIERGGERLERGGERIERGGDRDRKDNEGADSSWRVRREPDSQRAAAPKDSGAPQSRDDKWRRGGERDRDFRIDGARRDRDDGPRRDRDDGPRRDRDDERGGFRRADGARRTDEPQRETGGNWRDAPRHADRETRRPAERRDRDVRETRGDQRGSAPKEAASGGVGGNWRTAPATREEKPAAKRDQAQEKENKAGDDGEWTSVKRR

Target ORF information:

RefSeq Version	NM_141213.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 3 subunit a (eIF3a), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141213.4

ORF Insert Sequence:

ATGGCCCGCT ATACGCAACG TCCGGAAAAC GCCCTAAAAA GGGCTAATGA GTTCATTGAA 
GTGGGAAAGC CGCTTAGGGC GCTGGACACG CTGCAGGAAG TTTTCCGGAA CAAGCGATGG 
AACTATGCCT ACTCGGAGAC GGTCATCGAA CCGCTCATGT TCAAGTACCT GTACCTCTGC 
GTGGAACTCA AGAAGTCGCA CATCGCCAAG GAGGGACTAT TCCAGTACCG GAACATGTTC 
CAGCTGGTGA ACGTGAACTC TCTGGAAAAT GTAATCCGCG GCTACCTGAA AATGGCCGAA 
GAGCACACAG AGGCTGCCCA AGCCCAGTCC TCGGCGGCCG TTGCTGTTCT GGAGCTGGAT 
GACCTGGACA ACATTGCCAC GCCAGAGAGC ATCCTAATGA GCGCAGTGTG TGGCGAGGAC 
GCACAGGACC GCTCCGACCG CACTATTCTG CTCCCGTGGG TCAAGTTCCT GTGGGAGTCC 
TACTGCCAGT GTCTAGAGCT GCTGCGCGTA AACACGCATT GCGAGGCGCT GTACCACGAT 
ATCGCTCGCA TGGCCTTCCA GTTCTGCCTT AAGTACAACC GCAAGAGCGA GTTTCGCCGC 
TTGTGCGACA AGCTGCGCAA ACACTTGGAG GACATTTGCA AGAGCAGCAA CCAGACCACC 
GGTGTCTCGA TCAACAAGGT GGAAACGCAG CAGCTCTGCT TGGACACCAG GTTGTACCTT 
CTTGACTCGG CCATCCAGAT GGAGCTGTGG CAAGAGGCCT ACAAGGCAAT CGAGGATATC 
CATGGCCTGA TGGCTCTGTC CAAGAAGACA CCCGTGCCCA AAACCATGGC CAACTACTAC 
CAAAAATTAG CCATGGTCTT TAGCAAGGCC GGCAACCAGT TGTTCCACGC TGCAGCGCTG 
CTGAAGCTCT TCCAGCTGAC GCGCGAGCTG AAGAAGAACC TGACGAAGGA CGACTTGCAG 
CGTATGGCTG CCCACGTTCT CCTGGCCACT CTTTCTATTC CGTTGCCTTC GGCCCATCCA 
GAGTTCGACA GGTTTATTGA GGCGGACAAG AGCCCCCTGG AGAAGGCGCA GAAGTTGGCC 
GTGCTTTTGG GCCTGCCCCA GCCACCTACT CGCGTCTCCC TTATTCGTGA AGTGGTGCGC 
TTGAATGTTC CTCAACTCGT ATCTGAGGAC TTTCGCAATC TATACAACTG GTTGGAGGTG 
GATTTCAACC CACTGAATCT GTGCAAGCGC ATTCAGTCTA TCGTGGACTT CATTGAGAAC 
GGGCCCGAAA ACGCTCTGCT CACTCCTTAC ATTCAATCGC TGAAGGACGT GACTATAATG 
CGTCTGATTC GTCAGATCTC TCAGGTTTAT GAGAGTATTA AGTTCCAGCG TCTCTTGCAG 
CTGGCCAGTT TTTGCAACAT TTTTGAATTG GAAAAACTGC TGGTCGAGTC CGTGCGTCAC 
AATGACATGC AGATTCGTAT TGACCACCAG AAGAACAGTA TCTATTTCGG CACTGATCTG 
ACCGAGAGTC AACGCGAGTA CCGCCCCGAC GGACCCGCTT TGCAATCGAT GCCCTCGGAA 
CAGATCCGCT CTCAGTTGGT TAATATGTCT ACTGTTCTGA CACGGGCAGT TTCAATCGTC 
TACCCGAACC GTGAACGTGA CCAGCGGGCC AAGCTCCGCA ACCAGATGGT AAACCATTAC 
CATGAGATCA AGGATCGCGA GCACCAGCGT ATTCTTCAGC GACAGAAGAT CATCGAGGAC 
AGAAAGGAGT ACATCGAGAA GCAGAATAAT GCTCGTGAAG AGGAAGAGGC GCGGCGTCAG 
GAGGAGGAAT CGCGGAAGGC CAAACTGGCC GAACAGAAGC GCCTCGAGCA GGAGCAAGAA 
GAAAGAGAGC GCAAGCGCCA TCAGAACGAA ATCCAAGCCA TCCGGGAGAA GAGTCTCAAG 
GAGAAGGTTC AGCAAATCTC GCAGACGGCT CATGGTAAGA AGATGCTTTC CAAGCTGGAT 
GAGGAAGGCA TTAAGAAGTT GGACGCCGAG CAGATCGCCA AGCGGGAAAG CGAGGAACTT 
CAGCGCGAGG CCAAGGAACT GCAGTCGAAA CTCAAATCGC AAGAGAAGAA AATCGACTAC 
TTCGAACGCG CCAAGCGCCT GGAGGAGATA CCGCTTTTCG AAAAATACTT GGCCGAAAAG 
CAGGTTAAGG ACAAGGAGTT CTGGGAAGCA ACCGAGAAGA CACGCATCGA GAATGCCATT 
GCCGAACGGA AGGATGCCGT CGCCCAGCAG GAGCGTCTCA AGCGCATGTA TCCGGACCGG 
GATGAATTCC TTGAGGCCCT TAAAAAAGAA CGTGCCTCCC TCTACGTCGA AAAGCTCAAA 
AAGTTTGAGG CTGCTCTGGA GGCAGAGCGC AAAAAGCGCT TAGCCGACCG CATTATTCGA 
CGCCGCGAGG AGCGCCGACA GGCCTTCCTT CGCGAGAAAG AGGAGGAGCG TCTTCGCAAG 
GAGGAAGAGA TTCGCTTGGC ACAGGCTGCC GAAGAGCGCG CCGCAGCAGA AGCCCGCCGT 
TTGGAGCGTG AGGCTGAGGA CGAAAAGCGT CGTGCCCAAT ACGAAAAGCA GCGTGCCAAG 
GAGGAGGAAG CTGAGCGTAA AATCAAGGAA GATCGAGACC GCCTGTCCAG GGAGTTGGCC 
TCGGAGCGCG AACGCACTGA AAAGGACCGT GACACATGGC GTCCTCGAGG CGGTGACCGT 
CCAAGCGCAT CTAACGGCGG ATCCAGCGAG TGGCGTCGTG CTGCACCTGC GGCCAGTGAA 
CGCAACGACC GTGGTGGCGA GCGTATCGAG CGCGGAGGAG AACGGATCGA GCGCGGTGGA 
GAACGCCTCG AGCGTGGTGG AGAGCGCATT GAACGAGGCG GTGACCGTGA CCGCAAGGAC 
AACGAGGGAG CGGACTCCTC TTGGCGTGTG CGACGAGAGC CAGATTCACA GCGTGCAGCT 
GCTCCCAAAG ACAGTGGCGC TCCGCAATCG CGCGATGACA AGTGGCGGCG TGGCGGAGAA 
CGTGACCGCG ATTTCCGCAT TGATGGGGCT CGTCGCGACA GGGATGATGG GCCTCGGCGT 
GACCGTGACG ATGGTCCTCG CCGGGATCGT GACGATGAGC GCGGTGGATT CCGCCGCGCC 
GACGGGGCGC GTCGTACCGA CGAGCCTCAG CGGGAAACTG GAGGGAACTG GCGCGACGCT 
CCACGCCACG CTGATCGGGA AACCCGTCGC CCAGCTGAGA GGCGCGACCG AGATGTGCGC 
GAGACTCGTG GCGATCAACG CGGCTCTGCT CCTAAGGAGG CAGCCAGTGG CGGAGTAGGC 
GGAAACTGGC GTACTGCTCC TGCGACCCGT GAGGAAAAAC CAGCAGCTAA GCGCGATCAA 
GCCCAGGAAA AGGAGAACAA AGCTGGCGAT GATGGCGAGT GGACCAGCGT TAAGCGTCGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eIF3a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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