beta-Man cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	beta-Man
Entrez Gene ID	40524
Full Name	beta-Mannosidase
Synonyms	CG12582,CG12583,Dmel\CG12582
General protein information	Preferred Names beta-Mannosidase Names CG12582 gene product from transcript CG12582-RG CG12582-PA CG12582-PD CG12582-PF CG12582-PG CG12582-PH beta-Man-PA beta-Man-PD beta-Man-PF beta-Man-PG beta-Man-PH
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 81F6-81F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_164315.4	NP_730783.3	beta-Mannosidase, isoform F
NM_141179.4	NP_649436.2	beta-Mannosidase, isoform A
NM_001275312.1	NP_001262241.1	beta-Mannosidase, isoform G
NM_001275313.1	NP_001262242.1	beta-Mannosidase, isoform H
NM_001259970.2	NP_001246899.1	beta-Mannosidase, isoform D

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	manba	NP_956453.1
Magnaporthe oryzae (rice blast fungus)	MGG_01178	XP_003714062.1
Neurospora crassa	NCU00890	XP_964806.1
Pan troglodytes (chimpanzee)	MANBA	XP_001169005.1
	MANBA	XP_001101549.2
Drosophila melanogaster (fruit fly)	CG12582	NP_649436.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000143	XP_311001.5
Xenopus tropicalis (tropical clawed frog)	manba	XP_002934804.2
Homo sapiens (human)	MANBA	NP_005899.3
Bos taurus (cattle)	MANBA	NP_776812.1
Gallus gallus (chicken)	MANBA	XP_420666.3
Danio rerio (zebrafish)	LOC100331766	XP_002664183.1
Caenorhabditis elegans (roundworm)	C33G3.4	NP_510342.1
Canis lupus familiaris (dog)	MANBA	XP_005639293.1
Mus musculus (house mouse)	Manba	NP_081564.3
Rattus norvegicus (Norway rat)	Manba	NP_001026825.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following beta-Man gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the beta-Man cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03785	NM_141179.4 Latest version!	Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03785	NM_001259970.2 Latest version!	Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03786	NM_164315.4 Latest version!	Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa03786	NM_001275312.1 Latest version!	Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa07848	NM_001275313.1 Latest version!	Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

beta-Man ( NM_141179.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649436.2 Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03785
Clone ID Related Accession (Same CDS sequence)	NM_141179.4 , NM_001259970.2
Accession Version	NM_141179.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta-Mannosidase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141179.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTACG GATGCGGATT ACTCAGGAAT GGCACCACGG GCGCTGTTAT GGTCGGATTA 
GTTTGTGGCC TTTATGTTTG CAGCGGCCAA TGTGATAAGG TGGAAGTAAT AGAGCTGAGC 
AATTGGACGA TAACTAATCA GAATGGCTCC CTGACTATTG CCAACCAAAC GTTGCCCTTG 
GGCATATACA GTGCCTTCGC ACCCCAAGTG CTGGACTCCT ACAACGACGT CGGTCTGCGT 
TACTTGGCCT ACGACAACTT TACCTTCTCC AATTTCTTTC AGTTCGACGT TCATCACTTC 
AATCGCGGCC ACATCAACCT GACGTTTCAC GGCATTGACA CGGTGTCGGA AATCCGGCTG 
AACCATCAAC TGCTGGGACG CACGGACAAC ATGTTTGTCC GCTACTCTTA CGAAGTGAGT 
TCCCTGCTCC AAGCGGAAAA CTTTCTGGAA GTTGAGATCC AATCTCCAGT TGTGGCTGCT 
TTGGCAAGAG CCAATGCTTT AAAAGAAGCT AAAAAGAGCG TGCCTCCCGA ATGCCCCAAC 
GAGCGCTACC ATGGTGAGTG CCACATGAAC ATGCTGCGGA AGATGCAGGC TAGCTTTTCC 
TGGGACTGGG GTCCTGCAGC TCCCTCTGCT GGAATCTGGA AAAACGTGGA GCTGGAAATC 
TACGAGGTGG CGGTTATTCG TGAGGTGGAT GTGGACGTTA GCCGCGTCAA TGGTTCGTAC 
TGGAACATGC ATATTCGCTG CTTCCTGGAC GCGGGAGGCT GGCAAAACTT CAATGGAAGA 
CTCGTCCTAT ATGCTGTCGA GCTATTGGAC CGTCCGGTAG TAGTAGACAG ATACGCAATA 
AAAAACATCA GTCACTTGGC GCCAGTAATT GAATTCGATC AGGCCGTGCC AAATGGGAAA 
GTCGTAACCT GGTGGCCCAA TGGCTACGGC GAGCAGAAAC TGTATCCGCT CCACTTCACT 
CTGAAGACCT GGCTGGGACC TGATGGACCC GAGGTTCGCT CTAAGACCAA GTCGCACAAG 
TCGCTCCGAG TGGGTTTTCG TACGCTTGAT CTAGTAGAGG ATCCCGCCCC TGACGGCGTT 
GGAAACACCT TCCTGTTTCG AGTTAATGGT GTAGAAATGT TTATGAAAGG CAGTAACTAC 
ATACCATCCC ACTTATTACC CGAAATGCAA ACAAATGAAC AGATTTCACA TTTATTAAGG 
TCCGCCAAGG AAGCCCATAT GAACATGCTT CGAGTCTGGG GAGGCGGAGT TTACGAATCG 
GACTACTTTT ACCAGCTCGC CGACAGCTTG GGTCTTCTAA TTTGGCAAGA TATGATGTTT 
GCTTGTGCCA TGTACCCGGT TAACGACGAA TTCCTATCGT CAGTGCGTGA GGAGGTTCGC 
CAGAATGCCA TGCGACTATC ACACCATCCA AGCGTAGCCA TTTTTGTGAC CAATAACGAA 
AACGAAGCTG CTTTGGTACA GAACTGGTAC GGCACGCTTT TCGAAAGAGA TCGATTTGAG 
AGCGAGTACC GGGAGCTCTA CCTTGCTAAT GTCATCCACG AGTTGAAGCT TGTTTCGCAT 
AGCTCTCGAC CTCAGCCACT GGTCTCCTCA CCTTCAAATG GCAAAGCTAG TGAACCGGAC 
AACTATATTT CGAGCAACCC TCAGGATAAC CAAAATGGCG ATGTACATTT CTACGACTAC 
ACTAAGGACG GCTGGGATCC AGGAATTTTC CCACGTCCTC GATTCGTCAG TGAGTTCGGC 
TTTCAGAGCT TTCCAGGCGC ATACGCATGG CAGCGCAGCA AGGGCGAGGA CGATGACCTC 
CTCGGCCTGA TCACACACCG ACAACACCAC CCACTGGGAA ATGTTCCTGT TATCGCACTA 
GTGGAGAGAC ACCTACCGCT GCCCTTTCCA GAGAATGAGA ACTACGCGAC AGCACTGATT 
TACTTCAGCC AGATTGCTCA AGCCATGGCC ACAAAGGTGG AAACCGAATT ATACCGTAGC 
CTTCGGGACA CTCCCCACAG AACGATGGGG GCCCTTTACT GGCAGCTGAA TGATGTATGG 
GTGGCGCCAT CCTGGTCCAG CATTGATTTC TACGGCAACT GGAAGCTGCT CCATTACTGG 
GCACGTGATT TTCTAGCGCC TATTTCAATC GTTGCTCTTT ACGAAAAGTC CACGGATTCG 
TTAAACATCT CCCTTATTTG TGACCAATTG GAGGTGGACC CAAGGGAACT GAATGTCGTA 
GCTAACGTTC ACCTGTGGTC CCAGCTTCTG CCTCGGCAGT CCACCTCCTG GGATGTTACC 
CTGCGCCCCA ATGGCGTGCA GTACGACAAG GTCATTGCCA TCTCCGATCT GCTGCAAGGA 
GAGTTCACCA GAACTAACGC GTTTTTGGAG CTGCAGTTGC GCCGCGGGCA GGATGTGATC 
TCTAGGACCC ACTTCTTTCC CAGTAGCATT GCCGGAGCAG TCGGAATAGA GGACCCCGGG 
CTTGAGTTCG AGATTGCCTC CCGTACCTGT CTTAACACCA CAAACGTCGT CCGCAACAGC 
GTCAGCATTA GCATACAGGT GAAGCGCCCC GCCGTATTTG TATACCTAGA GCTGCTCAAG 
CCATACCGCT ACACCCTGTC CGAGAACGGA TTTATGCAGA CTGAGCCAAT GCATGTCGTC 
TACCTAACGT TCGACGCACC CTTGTGCGCC CGCCTTTTGC GCAAGTCGGA CCTCCGTGTG 
CTGACCGTCA ACGACTTTAT GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649436.2
CDS	210..2936
Translation	MKYGCGLLRNGTTGAVMVGLVCGLYVCSGQCDKVEVIELSNWTITNQNGSLTIANQTLPLGIYSAFAPQVLDSYNDVGLRYLAYDNFTFSNFFQFDVHHFNRGHINLTFHGIDTVSEIRLNHQLLGRTDNMFVRYSYEVSSLLQAENFLEVEIQSPVVAALARANALKEAKKSVPPECPNERYHGECHMNMLRKMQASFSWDWGPAAPSAGIWKNVELEIYEVAVIREVDVDVSRVNGSYWNMHIRCFLDAGGWQNFNGRLVLYAVELLDRPVVVDRYAIKNISHLAPVIEFDQAVPNGKVVTWWPNGYGEQKLYPLHFTLKTWLGPDGPEVRSKTKSHKSLRVGFRTLDLVEDPAPDGVGNTFLFRVNGVEMFMKGSNYIPSHLLPEMQTNEQISHLLRSAKEAHMNMLRVWGGGVYESDYFYQLADSLGLLIWQDMMFACAMYPVNDEFLSSVREEVRQNAMRLSHHPSVAIFVTNNENEAALVQNWYGTLFERDRFESEYRELYLANVIHELKLVSHSSRPQPLVSSPSNGKASEPDNYISSNPQDNQNGDVHFYDYTKDGWDPGIFPRPRFVSEFGFQSFPGAYAWQRSKGEDDDLLGLITHRQHHPLGNVPVIALVERHLPLPFPENENYATALIYFSQIAQAMATKVETELYRSLRDTPHRTMGALYWQLNDVWVAPSWSSIDFYGNWKLLHYWARDFLAPISIVALYEKSTDSLNISLICDQLEVDPRELNVVANVHLWSQLLPRQSTSWDVTLRPNGVQYDKVIAISDLLQGEFTRTNAFLELQLRRGQDVISRTHFFPSSIAGAVGIEDPGLEFEIASRTCLNTTNVVRNSVSISIQVKRPAVFVYLELLKPYRYTLSENGFMQTEPMHVVYLTFDAPLCARLLRKSDLRVLTVNDFMR

Target ORF information:

RefSeq Version	NM_141179.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141179.4

ORF Insert Sequence:

ATGAAGTACG GATGCGGATT ACTCAGGAAT GGCACCACGG GCGCTGTTAT GGTCGGATTA 
GTTTGTGGCC TTTATGTTTG CAGCGGCCAA TGTGATAAGG TGGAAGTAAT AGAGCTGAGC 
AATTGGACGA TAACTAATCA GAATGGCTCC CTGACTATTG CCAACCAAAC GTTGCCCTTG 
GGCATATACA GTGCCTTCGC ACCCCAAGTG CTGGACTCCT ACAACGACGT CGGTCTGCGT 
TACTTGGCCT ACGACAACTT TACCTTCTCC AATTTCTTTC AGTTCGACGT TCATCACTTC 
AATCGCGGCC ACATCAACCT GACGTTTCAC GGCATTGACA CGGTGTCGGA AATCCGGCTG 
AACCATCAAC TGCTGGGACG CACGGACAAC ATGTTTGTCC GCTACTCTTA CGAAGTGAGT 
TCCCTGCTCC AAGCGGAAAA CTTTCTGGAA GTTGAGATCC AATCTCCAGT TGTGGCTGCT 
TTGGCAAGAG CCAATGCTTT AAAAGAAGCT AAAAAGAGCG TGCCTCCCGA ATGCCCCAAC 
GAGCGCTACC ATGGTGAGTG CCACATGAAC ATGCTGCGGA AGATGCAGGC TAGCTTTTCC 
TGGGACTGGG GTCCTGCAGC TCCCTCTGCT GGAATCTGGA AAAACGTGGA GCTGGAAATC 
TACGAGGTGG CGGTTATTCG TGAGGTGGAT GTGGACGTTA GCCGCGTCAA TGGTTCGTAC 
TGGAACATGC ATATTCGCTG CTTCCTGGAC GCGGGAGGCT GGCAAAACTT CAATGGAAGA 
CTCGTCCTAT ATGCTGTCGA GCTATTGGAC CGTCCGGTAG TAGTAGACAG ATACGCAATA 
AAAAACATCA GTCACTTGGC GCCAGTAATT GAATTCGATC AGGCCGTGCC AAATGGGAAA 
GTCGTAACCT GGTGGCCCAA TGGCTACGGC GAGCAGAAAC TGTATCCGCT CCACTTCACT 
CTGAAGACCT GGCTGGGACC TGATGGACCC GAGGTTCGCT CTAAGACCAA GTCGCACAAG 
TCGCTCCGAG TGGGTTTTCG TACGCTTGAT CTAGTAGAGG ATCCCGCCCC TGACGGCGTT 
GGAAACACCT TCCTGTTTCG AGTTAATGGT GTAGAAATGT TTATGAAAGG CAGTAACTAC 
ATACCATCCC ACTTATTACC CGAAATGCAA ACAAATGAAC AGATTTCACA TTTATTAAGG 
TCCGCCAAGG AAGCCCATAT GAACATGCTT CGAGTCTGGG GAGGCGGAGT TTACGAATCG 
GACTACTTTT ACCAGCTCGC CGACAGCTTG GGTCTTCTAA TTTGGCAAGA TATGATGTTT 
GCTTGTGCCA TGTACCCGGT TAACGACGAA TTCCTATCGT CAGTGCGTGA GGAGGTTCGC 
CAGAATGCCA TGCGACTATC ACACCATCCA AGCGTAGCCA TTTTTGTGAC CAATAACGAA 
AACGAAGCTG CTTTGGTACA GAACTGGTAC GGCACGCTTT TCGAAAGAGA TCGATTTGAG 
AGCGAGTACC GGGAGCTCTA CCTTGCTAAT GTCATCCACG AGTTGAAGCT TGTTTCGCAT 
AGCTCTCGAC CTCAGCCACT GGTCTCCTCA CCTTCAAATG GCAAAGCTAG TGAACCGGAC 
AACTATATTT CGAGCAACCC TCAGGATAAC CAAAATGGCG ATGTACATTT CTACGACTAC 
ACTAAGGACG GCTGGGATCC AGGAATTTTC CCACGTCCTC GATTCGTCAG TGAGTTCGGC 
TTTCAGAGCT TTCCAGGCGC ATACGCATGG CAGCGCAGCA AGGGCGAGGA CGATGACCTC 
CTCGGCCTGA TCACACACCG ACAACACCAC CCACTGGGAA ATGTTCCTGT TATCGCACTA 
GTGGAGAGAC ACCTACCGCT GCCCTTTCCA GAGAATGAGA ACTACGCGAC AGCACTGATT 
TACTTCAGCC AGATTGCTCA AGCCATGGCC ACAAAGGTGG AAACCGAATT ATACCGTAGC 
CTTCGGGACA CTCCCCACAG AACGATGGGG GCCCTTTACT GGCAGCTGAA TGATGTATGG 
GTGGCGCCAT CCTGGTCCAG CATTGATTTC TACGGCAACT GGAAGCTGCT CCATTACTGG 
GCACGTGATT TTCTAGCGCC TATTTCAATC GTTGCTCTTT ACGAAAAGTC CACGGATTCG 
TTAAACATCT CCCTTATTTG TGACCAATTG GAGGTGGACC CAAGGGAACT GAATGTCGTA 
GCTAACGTTC ACCTGTGGTC CCAGCTTCTG CCTCGGCAGT CCACCTCCTG GGATGTTACC 
CTGCGCCCCA ATGGCGTGCA GTACGACAAG GTCATTGCCA TCTCCGATCT GCTGCAAGGA 
GAGTTCACCA GAACTAACGC GTTTTTGGAG CTGCAGTTGC GCCGCGGGCA GGATGTGATC 
TCTAGGACCC ACTTCTTTCC CAGTAGCATT GCCGGAGCAG TCGGAATAGA GGACCCCGGG 
CTTGAGTTCG AGATTGCCTC CCGTACCTGT CTTAACACCA CAAACGTCGT CCGCAACAGC 
GTCAGCATTA GCATACAGGT GAAGCGCCCC GCCGTATTTG TATACCTAGA GCTGCTCAAG 
CCATACCGCT ACACCCTGTC CGAGAACGGA TTTATGCAGA CTGAGCCAAT GCATGTCGTC 
TACCTAACGT TCGACGCACC CTTGTGCGCC CGCCTTTTGC GCAAGTCGGA CCTCCGTGTG 
CTGACCGTCA ACGACTTTAT GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

beta-Man ( NM_001259970.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246899.1 Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa03785
Clone ID Related Accession (Same CDS sequence)	NM_141179.4 , NM_001259970.2
Accession Version	NM_001259970.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta-Mannosidase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001259970.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTACG GATGCGGATT ACTCAGGAAT GGCACCACGG GCGCTGTTAT GGTCGGATTA 
GTTTGTGGCC TTTATGTTTG CAGCGGCCAA TGTGATAAGG TGGAAGTAAT AGAGCTGAGC 
AATTGGACGA TAACTAATCA GAATGGCTCC CTGACTATTG CCAACCAAAC GTTGCCCTTG 
GGCATATACA GTGCCTTCGC ACCCCAAGTG CTGGACTCCT ACAACGACGT CGGTCTGCGT 
TACTTGGCCT ACGACAACTT TACCTTCTCC AATTTCTTTC AGTTCGACGT TCATCACTTC 
AATCGCGGCC ACATCAACCT GACGTTTCAC GGCATTGACA CGGTGTCGGA AATCCGGCTG 
AACCATCAAC TGCTGGGACG CACGGACAAC ATGTTTGTCC GCTACTCTTA CGAAGTGAGT 
TCCCTGCTCC AAGCGGAAAA CTTTCTGGAA GTTGAGATCC AATCTCCAGT TGTGGCTGCT 
TTGGCAAGAG CCAATGCTTT AAAAGAAGCT AAAAAGAGCG TGCCTCCCGA ATGCCCCAAC 
GAGCGCTACC ATGGTGAGTG CCACATGAAC ATGCTGCGGA AGATGCAGGC TAGCTTTTCC 
TGGGACTGGG GTCCTGCAGC TCCCTCTGCT GGAATCTGGA AAAACGTGGA GCTGGAAATC 
TACGAGGTGG CGGTTATTCG TGAGGTGGAT GTGGACGTTA GCCGCGTCAA TGGTTCGTAC 
TGGAACATGC ATATTCGCTG CTTCCTGGAC GCGGGAGGCT GGCAAAACTT CAATGGAAGA 
CTCGTCCTAT ATGCTGTCGA GCTATTGGAC CGTCCGGTAG TAGTAGACAG ATACGCAATA 
AAAAACATCA GTCACTTGGC GCCAGTAATT GAATTCGATC AGGCCGTGCC AAATGGGAAA 
GTCGTAACCT GGTGGCCCAA TGGCTACGGC GAGCAGAAAC TGTATCCGCT CCACTTCACT 
CTGAAGACCT GGCTGGGACC TGATGGACCC GAGGTTCGCT CTAAGACCAA GTCGCACAAG 
TCGCTCCGAG TGGGTTTTCG TACGCTTGAT CTAGTAGAGG ATCCCGCCCC TGACGGCGTT 
GGAAACACCT TCCTGTTTCG AGTTAATGGT GTAGAAATGT TTATGAAAGG CAGTAACTAC 
ATACCATCCC ACTTATTACC CGAAATGCAA ACAAATGAAC AGATTTCACA TTTATTAAGG 
TCCGCCAAGG AAGCCCATAT GAACATGCTT CGAGTCTGGG GAGGCGGAGT TTACGAATCG 
GACTACTTTT ACCAGCTCGC CGACAGCTTG GGTCTTCTAA TTTGGCAAGA TATGATGTTT 
GCTTGTGCCA TGTACCCGGT TAACGACGAA TTCCTATCGT CAGTGCGTGA GGAGGTTCGC 
CAGAATGCCA TGCGACTATC ACACCATCCA AGCGTAGCCA TTTTTGTGAC CAATAACGAA 
AACGAAGCTG CTTTGGTACA GAACTGGTAC GGCACGCTTT TCGAAAGAGA TCGATTTGAG 
AGCGAGTACC GGGAGCTCTA CCTTGCTAAT GTCATCCACG AGTTGAAGCT TGTTTCGCAT 
AGCTCTCGAC CTCAGCCACT GGTCTCCTCA CCTTCAAATG GCAAAGCTAG TGAACCGGAC 
AACTATATTT CGAGCAACCC TCAGGATAAC CAAAATGGCG ATGTACATTT CTACGACTAC 
ACTAAGGACG GCTGGGATCC AGGAATTTTC CCACGTCCTC GATTCGTCAG TGAGTTCGGC 
TTTCAGAGCT TTCCAGGCGC ATACGCATGG CAGCGCAGCA AGGGCGAGGA CGATGACCTC 
CTCGGCCTGA TCACACACCG ACAACACCAC CCACTGGGAA ATGTTCCTGT TATCGCACTA 
GTGGAGAGAC ACCTACCGCT GCCCTTTCCA GAGAATGAGA ACTACGCGAC AGCACTGATT 
TACTTCAGCC AGATTGCTCA AGCCATGGCC ACAAAGGTGG AAACCGAATT ATACCGTAGC 
CTTCGGGACA CTCCCCACAG AACGATGGGG GCCCTTTACT GGCAGCTGAA TGATGTATGG 
GTGGCGCCAT CCTGGTCCAG CATTGATTTC TACGGCAACT GGAAGCTGCT CCATTACTGG 
GCACGTGATT TTCTAGCGCC TATTTCAATC GTTGCTCTTT ACGAAAAGTC CACGGATTCG 
TTAAACATCT CCCTTATTTG TGACCAATTG GAGGTGGACC CAAGGGAACT GAATGTCGTA 
GCTAACGTTC ACCTGTGGTC CCAGCTTCTG CCTCGGCAGT CCACCTCCTG GGATGTTACC 
CTGCGCCCCA ATGGCGTGCA GTACGACAAG GTCATTGCCA TCTCCGATCT GCTGCAAGGA 
GAGTTCACCA GAACTAACGC GTTTTTGGAG CTGCAGTTGC GCCGCGGGCA GGATGTGATC 
TCTAGGACCC ACTTCTTTCC CAGTAGCATT GCCGGAGCAG TCGGAATAGA GGACCCCGGG 
CTTGAGTTCG AGATTGCCTC CCGTACCTGT CTTAACACCA CAAACGTCGT CCGCAACAGC 
GTCAGCATTA GCATACAGGT GAAGCGCCCC GCCGTATTTG TATACCTAGA GCTGCTCAAG 
CCATACCGCT ACACCCTGTC CGAGAACGGA TTTATGCAGA CTGAGCCAAT GCATGTCGTC 
TACCTAACGT TCGACGCACC CTTGTGCGCC CGCCTTTTGC GCAAGTCGGA CCTCCGTGTG 
CTGACCGTCA ACGACTTTAT GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246899.1
CDS	97..2823
Translation	MKYGCGLLRNGTTGAVMVGLVCGLYVCSGQCDKVEVIELSNWTITNQNGSLTIANQTLPLGIYSAFAPQVLDSYNDVGLRYLAYDNFTFSNFFQFDVHHFNRGHINLTFHGIDTVSEIRLNHQLLGRTDNMFVRYSYEVSSLLQAENFLEVEIQSPVVAALARANALKEAKKSVPPECPNERYHGECHMNMLRKMQASFSWDWGPAAPSAGIWKNVELEIYEVAVIREVDVDVSRVNGSYWNMHIRCFLDAGGWQNFNGRLVLYAVELLDRPVVVDRYAIKNISHLAPVIEFDQAVPNGKVVTWWPNGYGEQKLYPLHFTLKTWLGPDGPEVRSKTKSHKSLRVGFRTLDLVEDPAPDGVGNTFLFRVNGVEMFMKGSNYIPSHLLPEMQTNEQISHLLRSAKEAHMNMLRVWGGGVYESDYFYQLADSLGLLIWQDMMFACAMYPVNDEFLSSVREEVRQNAMRLSHHPSVAIFVTNNENEAALVQNWYGTLFERDRFESEYRELYLANVIHELKLVSHSSRPQPLVSSPSNGKASEPDNYISSNPQDNQNGDVHFYDYTKDGWDPGIFPRPRFVSEFGFQSFPGAYAWQRSKGEDDDLLGLITHRQHHPLGNVPVIALVERHLPLPFPENENYATALIYFSQIAQAMATKVETELYRSLRDTPHRTMGALYWQLNDVWVAPSWSSIDFYGNWKLLHYWARDFLAPISIVALYEKSTDSLNISLICDQLEVDPRELNVVANVHLWSQLLPRQSTSWDVTLRPNGVQYDKVIAISDLLQGEFTRTNAFLELQLRRGQDVISRTHFFPSSIAGAVGIEDPGLEFEIASRTCLNTTNVVRNSVSISIQVKRPAVFVYLELLKPYRYTLSENGFMQTEPMHVVYLTFDAPLCARLLRKSDLRVLTVNDFMR

Target ORF information:

RefSeq Version	NM_001259970.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259970.2

ORF Insert Sequence:

ATGAAGTACG GATGCGGATT ACTCAGGAAT GGCACCACGG GCGCTGTTAT GGTCGGATTA 
GTTTGTGGCC TTTATGTTTG CAGCGGCCAA TGTGATAAGG TGGAAGTAAT AGAGCTGAGC 
AATTGGACGA TAACTAATCA GAATGGCTCC CTGACTATTG CCAACCAAAC GTTGCCCTTG 
GGCATATACA GTGCCTTCGC ACCCCAAGTG CTGGACTCCT ACAACGACGT CGGTCTGCGT 
TACTTGGCCT ACGACAACTT TACCTTCTCC AATTTCTTTC AGTTCGACGT TCATCACTTC 
AATCGCGGCC ACATCAACCT GACGTTTCAC GGCATTGACA CGGTGTCGGA AATCCGGCTG 
AACCATCAAC TGCTGGGACG CACGGACAAC ATGTTTGTCC GCTACTCTTA CGAAGTGAGT 
TCCCTGCTCC AAGCGGAAAA CTTTCTGGAA GTTGAGATCC AATCTCCAGT TGTGGCTGCT 
TTGGCAAGAG CCAATGCTTT AAAAGAAGCT AAAAAGAGCG TGCCTCCCGA ATGCCCCAAC 
GAGCGCTACC ATGGTGAGTG CCACATGAAC ATGCTGCGGA AGATGCAGGC TAGCTTTTCC 
TGGGACTGGG GTCCTGCAGC TCCCTCTGCT GGAATCTGGA AAAACGTGGA GCTGGAAATC 
TACGAGGTGG CGGTTATTCG TGAGGTGGAT GTGGACGTTA GCCGCGTCAA TGGTTCGTAC 
TGGAACATGC ATATTCGCTG CTTCCTGGAC GCGGGAGGCT GGCAAAACTT CAATGGAAGA 
CTCGTCCTAT ATGCTGTCGA GCTATTGGAC CGTCCGGTAG TAGTAGACAG ATACGCAATA 
AAAAACATCA GTCACTTGGC GCCAGTAATT GAATTCGATC AGGCCGTGCC AAATGGGAAA 
GTCGTAACCT GGTGGCCCAA TGGCTACGGC GAGCAGAAAC TGTATCCGCT CCACTTCACT 
CTGAAGACCT GGCTGGGACC TGATGGACCC GAGGTTCGCT CTAAGACCAA GTCGCACAAG 
TCGCTCCGAG TGGGTTTTCG TACGCTTGAT CTAGTAGAGG ATCCCGCCCC TGACGGCGTT 
GGAAACACCT TCCTGTTTCG AGTTAATGGT GTAGAAATGT TTATGAAAGG CAGTAACTAC 
ATACCATCCC ACTTATTACC CGAAATGCAA ACAAATGAAC AGATTTCACA TTTATTAAGG 
TCCGCCAAGG AAGCCCATAT GAACATGCTT CGAGTCTGGG GAGGCGGAGT TTACGAATCG 
GACTACTTTT ACCAGCTCGC CGACAGCTTG GGTCTTCTAA TTTGGCAAGA TATGATGTTT 
GCTTGTGCCA TGTACCCGGT TAACGACGAA TTCCTATCGT CAGTGCGTGA GGAGGTTCGC 
CAGAATGCCA TGCGACTATC ACACCATCCA AGCGTAGCCA TTTTTGTGAC CAATAACGAA 
AACGAAGCTG CTTTGGTACA GAACTGGTAC GGCACGCTTT TCGAAAGAGA TCGATTTGAG 
AGCGAGTACC GGGAGCTCTA CCTTGCTAAT GTCATCCACG AGTTGAAGCT TGTTTCGCAT 
AGCTCTCGAC CTCAGCCACT GGTCTCCTCA CCTTCAAATG GCAAAGCTAG TGAACCGGAC 
AACTATATTT CGAGCAACCC TCAGGATAAC CAAAATGGCG ATGTACATTT CTACGACTAC 
ACTAAGGACG GCTGGGATCC AGGAATTTTC CCACGTCCTC GATTCGTCAG TGAGTTCGGC 
TTTCAGAGCT TTCCAGGCGC ATACGCATGG CAGCGCAGCA AGGGCGAGGA CGATGACCTC 
CTCGGCCTGA TCACACACCG ACAACACCAC CCACTGGGAA ATGTTCCTGT TATCGCACTA 
GTGGAGAGAC ACCTACCGCT GCCCTTTCCA GAGAATGAGA ACTACGCGAC AGCACTGATT 
TACTTCAGCC AGATTGCTCA AGCCATGGCC ACAAAGGTGG AAACCGAATT ATACCGTAGC 
CTTCGGGACA CTCCCCACAG AACGATGGGG GCCCTTTACT GGCAGCTGAA TGATGTATGG 
GTGGCGCCAT CCTGGTCCAG CATTGATTTC TACGGCAACT GGAAGCTGCT CCATTACTGG 
GCACGTGATT TTCTAGCGCC TATTTCAATC GTTGCTCTTT ACGAAAAGTC CACGGATTCG 
TTAAACATCT CCCTTATTTG TGACCAATTG GAGGTGGACC CAAGGGAACT GAATGTCGTA 
GCTAACGTTC ACCTGTGGTC CCAGCTTCTG CCTCGGCAGT CCACCTCCTG GGATGTTACC 
CTGCGCCCCA ATGGCGTGCA GTACGACAAG GTCATTGCCA TCTCCGATCT GCTGCAAGGA 
GAGTTCACCA GAACTAACGC GTTTTTGGAG CTGCAGTTGC GCCGCGGGCA GGATGTGATC 
TCTAGGACCC ACTTCTTTCC CAGTAGCATT GCCGGAGCAG TCGGAATAGA GGACCCCGGG 
CTTGAGTTCG AGATTGCCTC CCGTACCTGT CTTAACACCA CAAACGTCGT CCGCAACAGC 
GTCAGCATTA GCATACAGGT GAAGCGCCCC GCCGTATTTG TATACCTAGA GCTGCTCAAG 
CCATACCGCT ACACCCTGTC CGAGAACGGA TTTATGCAGA CTGAGCCAAT GCATGTCGTC 
TACCTAACGT TCGACGCACC CTTGTGCGCC CGCCTTTTGC GCAAGTCGGA CCTCCGTGTG 
CTGACCGTCA ACGACTTTAT GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

beta-Man ( NM_164315.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730783.3 Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa03786
Clone ID Related Accession (Same CDS sequence)	NM_164315.4 , NM_001275312.1
Accession Version	NM_164315.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta-Mannosidase, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_164315.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGTCC GCTACTCTTA CGAAGTGAGT TCCCTGCTCC AAGCGGAAAA CTTTCTGGAA 
GTTGAGATCC AATCTCCAGT TGTGGCTGCT TTGGCAAGAG CCAATGCTTT AAAAGAAGCT 
AAAAAGAGCG TGCCTCCCGA ATGCCCCAAC GAGCGCTACC ATGGTGAGTG CCACATGAAC 
ATGCTGCGGA AGATGCAGGC TAGCTTTTCC TGGGACTGGG GTCCTGCAGC TCCCTCTGCT 
GGAATCTGGA AAAACGTGGA GCTGGAAATC TACGAGGTGG CGGTTATTCG TGAGGTGGAT 
GTGGACGTTA GCCGCGTCAA TGGTTCGTAC TGGAACATGC ATATTCGCTG CTTCCTGGAC 
GCGGGAGGCT GGCAAAACTT CAATGGAAGA CTCGTCCTAT ATGCTGTCGA GCTATTGGAC 
CGTCCGGTAG TAGTAGACAG ATACGCAATA AAAAACATCA GTCACTTGGC GCCAGTAATT 
GAATTCGATC AGGCCGTGCC AAATGGGAAA GTCGTAACCT GGTGGCCCAA TGGCTACGGC 
GAGCAGAAAC TGTATCCGCT CCACTTCACT CTGAAGACCT GGCTGGGACC TGATGGACCC 
GAGGTTCGCT CTAAGACCAA GTCGCACAAG TCGCTCCGAG TGGGTTTTCG TACGCTTGAT 
CTAGTAGAGG ATCCCGCCCC TGACGGCGTT GGAAACACCT TCCTGTTTCG AGTTAATGGT 
GTAGAAATGT TTATGAAAGG CAGTAACTAC ATACCATCCC ACTTATTACC CGAAATGCAA 
ACAAATGAAC AGATTTCACA TTTATTAAGG TCCGCCAAGG AAGCCCATAT GAACATGCTT 
CGAGTCTGGG GAGGCGGAGT TTACGAATCG GACTACTTTT ACCAGCTCGC CGACAGCTTG 
GGTCTTCTAA TTTGGCAAGA TATGATGTTT GCTTGTGCCA TGTACCCGGT TAACGACGAA 
TTCCTATCGT CAGTGCGTGA GGAGGTTCGC CAGAATGCCA TGCGACTATC ACACCATCCA 
AGCGTAGCCA TTTTTGTGAC CAATAACGAA AACGAAGCTG CTTTGGTACA GAACTGGTAC 
GGCACGCTTT TCGAAAGAGA TCGATTTGAG AGCGAGTACC GGGAGCTCTA CCTTGCTAAT 
GTCATCCACG AGTTGAAGCT TGTTTCGCAT AGCTCTCGAC CTCAGCCACT GGTCTCCTCA 
CCTTCAAATG GCAAAGCTAG TGAACCGGAC AACTATATTT CGAGCAACCC TCAGGATAAC 
CAAAATGGCG ATGTACATTT CTACGACTAC ACTAAGGACG GCTGGGATCC AGGAATTTTC 
CCACGTCCTC GATTCGTCAG TGAGTTCGGC TTTCAGAGCT TTCCAGGCGC ATACGCATGG 
CAGCGCAGCA AGGGCGAGGA CGATGACCTC CTCGGCCTGA TCACACACCG ACAACACCAC 
CCACTGGGAA ATGTTCCTGT TATCGCACTA GTGGAGAGAC ACCTACCGCT GCCCTTTCCA 
GAGAATGAGA ACTACGCGAC AGCACTGATT TACTTCAGCC AGATTGCTCA AGCCATGGCC 
ACAAAGGTGG AAACCGAATT ATACCGTAGC CTTCGGGACA CTCCCCACAG AACGATGGGG 
GCCCTTTACT GGCAGCTGAA TGATGTATGG GTGGCGCCAT CCTGGTCCAG CATTGATTTC 
TACGGCAACT GGAAGCTGCT CCATTACTGG GCACGTGATT TTCTAGCGCC TATTTCAATC 
GTTGCTCTTT ACGAAAAGTC CACGGATTCG TTAAACATCT CCCTTATTTG TGACCAATTG 
GAGGTGGACC CAAGGGAACT GAATGTCGTA GCTAACGTTC ACCTGTGGTC CCAGCTTCTG 
CCTCGGCAGT CCACCTCCTG GGATGTTACC CTGCGCCCCA ATGGCGTGCA GTACGACAAG 
GTCATTGCCA TCTCCGATCT GCTGCAAGGA GAGTTCACCA GAACTAACGC GTTTTTGGAG 
CTGCAGTTGC GCCGCGGGCA GGATGTGATC TCTAGGACCC ACTTCTTTCC CAGTAGCATT 
GCCGGAGCAG TCGGAATAGA GGACCCCGGG CTTGAGTTCG AGATTGCCTC CCGTACCTGT 
CTTAACACCA CAAACGTCGT CCGCAACAGC GTCAGCATTA GCATACAGGT GAAGCGCCCC 
GCCGTATTTG TATACCTAGA GCTGCTCAAG CCATACCGCT ACACCCTGTC CGAGAACGGA 
TTTATGCAGA CTGAGCCAAT GCATGTCGTC TACCTAACGT TCGACGCACC CTTGTGCGCC 
CGCCTTTTGC GCAAGTCGGA CCTCCGTGTG CTGACCGTCA ACGACTTTAT GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730783.3
CDS	672..3008
Translation	MFVRYSYEVSSLLQAENFLEVEIQSPVVAALARANALKEAKKSVPPECPNERYHGECHMNMLRKMQASFSWDWGPAAPSAGIWKNVELEIYEVAVIREVDVDVSRVNGSYWNMHIRCFLDAGGWQNFNGRLVLYAVELLDRPVVVDRYAIKNISHLAPVIEFDQAVPNGKVVTWWPNGYGEQKLYPLHFTLKTWLGPDGPEVRSKTKSHKSLRVGFRTLDLVEDPAPDGVGNTFLFRVNGVEMFMKGSNYIPSHLLPEMQTNEQISHLLRSAKEAHMNMLRVWGGGVYESDYFYQLADSLGLLIWQDMMFACAMYPVNDEFLSSVREEVRQNAMRLSHHPSVAIFVTNNENEAALVQNWYGTLFERDRFESEYRELYLANVIHELKLVSHSSRPQPLVSSPSNGKASEPDNYISSNPQDNQNGDVHFYDYTKDGWDPGIFPRPRFVSEFGFQSFPGAYAWQRSKGEDDDLLGLITHRQHHPLGNVPVIALVERHLPLPFPENENYATALIYFSQIAQAMATKVETELYRSLRDTPHRTMGALYWQLNDVWVAPSWSSIDFYGNWKLLHYWARDFLAPISIVALYEKSTDSLNISLICDQLEVDPRELNVVANVHLWSQLLPRQSTSWDVTLRPNGVQYDKVIAISDLLQGEFTRTNAFLELQLRRGQDVISRTHFFPSSIAGAVGIEDPGLEFEIASRTCLNTTNVVRNSVSISIQVKRPAVFVYLELLKPYRYTLSENGFMQTEPMHVVYLTFDAPLCARLLRKSDLRVLTVNDFMR

Target ORF information:

RefSeq Version	NM_164315.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164315.4

ORF Insert Sequence:

ATGTTTGTCC GCTACTCTTA CGAAGTGAGT TCCCTGCTCC AAGCGGAAAA CTTTCTGGAA 
GTTGAGATCC AATCTCCAGT TGTGGCTGCT TTGGCAAGAG CCAATGCTTT AAAAGAAGCT 
AAAAAGAGCG TGCCTCCCGA ATGCCCCAAC GAGCGCTACC ATGGTGAGTG CCACATGAAC 
ATGCTGCGGA AGATGCAGGC TAGCTTTTCC TGGGACTGGG GTCCTGCAGC TCCCTCTGCT 
GGAATCTGGA AAAACGTGGA GCTGGAAATC TACGAGGTGG CGGTTATTCG TGAGGTGGAT 
GTGGACGTTA GCCGCGTCAA TGGTTCGTAC TGGAACATGC ATATTCGCTG CTTCCTGGAC 
GCGGGAGGCT GGCAAAACTT CAATGGAAGA CTCGTCCTAT ATGCTGTCGA GCTATTGGAC 
CGTCCGGTAG TAGTAGACAG ATACGCAATA AAAAACATCA GTCACTTGGC GCCAGTAATT 
GAATTCGATC AGGCCGTGCC AAATGGGAAA GTCGTAACCT GGTGGCCCAA TGGCTACGGC 
GAGCAGAAAC TGTATCCGCT CCACTTCACT CTGAAGACCT GGCTGGGACC TGATGGACCC 
GAGGTTCGCT CTAAGACCAA GTCGCACAAG TCGCTCCGAG TGGGTTTTCG TACGCTTGAT 
CTAGTAGAGG ATCCCGCCCC TGACGGCGTT GGAAACACCT TCCTGTTTCG AGTTAATGGT 
GTAGAAATGT TTATGAAAGG CAGTAACTAC ATACCATCCC ACTTATTACC CGAAATGCAA 
ACAAATGAAC AGATTTCACA TTTATTAAGG TCCGCCAAGG AAGCCCATAT GAACATGCTT 
CGAGTCTGGG GAGGCGGAGT TTACGAATCG GACTACTTTT ACCAGCTCGC CGACAGCTTG 
GGTCTTCTAA TTTGGCAAGA TATGATGTTT GCTTGTGCCA TGTACCCGGT TAACGACGAA 
TTCCTATCGT CAGTGCGTGA GGAGGTTCGC CAGAATGCCA TGCGACTATC ACACCATCCA 
AGCGTAGCCA TTTTTGTGAC CAATAACGAA AACGAAGCTG CTTTGGTACA GAACTGGTAC 
GGCACGCTTT TCGAAAGAGA TCGATTTGAG AGCGAGTACC GGGAGCTCTA CCTTGCTAAT 
GTCATCCACG AGTTGAAGCT TGTTTCGCAT AGCTCTCGAC CTCAGCCACT GGTCTCCTCA 
CCTTCAAATG GCAAAGCTAG TGAACCGGAC AACTATATTT CGAGCAACCC TCAGGATAAC 
CAAAATGGCG ATGTACATTT CTACGACTAC ACTAAGGACG GCTGGGATCC AGGAATTTTC 
CCACGTCCTC GATTCGTCAG TGAGTTCGGC TTTCAGAGCT TTCCAGGCGC ATACGCATGG 
CAGCGCAGCA AGGGCGAGGA CGATGACCTC CTCGGCCTGA TCACACACCG ACAACACCAC 
CCACTGGGAA ATGTTCCTGT TATCGCACTA GTGGAGAGAC ACCTACCGCT GCCCTTTCCA 
GAGAATGAGA ACTACGCGAC AGCACTGATT TACTTCAGCC AGATTGCTCA AGCCATGGCC 
ACAAAGGTGG AAACCGAATT ATACCGTAGC CTTCGGGACA CTCCCCACAG AACGATGGGG 
GCCCTTTACT GGCAGCTGAA TGATGTATGG GTGGCGCCAT CCTGGTCCAG CATTGATTTC 
TACGGCAACT GGAAGCTGCT CCATTACTGG GCACGTGATT TTCTAGCGCC TATTTCAATC 
GTTGCTCTTT ACGAAAAGTC CACGGATTCG TTAAACATCT CCCTTATTTG TGACCAATTG 
GAGGTGGACC CAAGGGAACT GAATGTCGTA GCTAACGTTC ACCTGTGGTC CCAGCTTCTG 
CCTCGGCAGT CCACCTCCTG GGATGTTACC CTGCGCCCCA ATGGCGTGCA GTACGACAAG 
GTCATTGCCA TCTCCGATCT GCTGCAAGGA GAGTTCACCA GAACTAACGC GTTTTTGGAG 
CTGCAGTTGC GCCGCGGGCA GGATGTGATC TCTAGGACCC ACTTCTTTCC CAGTAGCATT 
GCCGGAGCAG TCGGAATAGA GGACCCCGGG CTTGAGTTCG AGATTGCCTC CCGTACCTGT 
CTTAACACCA CAAACGTCGT CCGCAACAGC GTCAGCATTA GCATACAGGT GAAGCGCCCC 
GCCGTATTTG TATACCTAGA GCTGCTCAAG CCATACCGCT ACACCCTGTC CGAGAACGGA 
TTTATGCAGA CTGAGCCAAT GCATGTCGTC TACCTAACGT TCGACGCACC CTTGTGCGCC 
CGCCTTTTGC GCAAGTCGGA CCTCCGTGTG CTGACCGTCA ACGACTTTAT GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

beta-Man ( NM_001275312.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262241.1 Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa03786
Clone ID Related Accession (Same CDS sequence)	NM_164315.4 , NM_001275312.1
Accession Version	NM_001275312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta-Mannosidase, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGTCC GCTACTCTTA CGAAGTGAGT TCCCTGCTCC AAGCGGAAAA CTTTCTGGAA 
GTTGAGATCC AATCTCCAGT TGTGGCTGCT TTGGCAAGAG CCAATGCTTT AAAAGAAGCT 
AAAAAGAGCG TGCCTCCCGA ATGCCCCAAC GAGCGCTACC ATGGTGAGTG CCACATGAAC 
ATGCTGCGGA AGATGCAGGC TAGCTTTTCC TGGGACTGGG GTCCTGCAGC TCCCTCTGCT 
GGAATCTGGA AAAACGTGGA GCTGGAAATC TACGAGGTGG CGGTTATTCG TGAGGTGGAT 
GTGGACGTTA GCCGCGTCAA TGGTTCGTAC TGGAACATGC ATATTCGCTG CTTCCTGGAC 
GCGGGAGGCT GGCAAAACTT CAATGGAAGA CTCGTCCTAT ATGCTGTCGA GCTATTGGAC 
CGTCCGGTAG TAGTAGACAG ATACGCAATA AAAAACATCA GTCACTTGGC GCCAGTAATT 
GAATTCGATC AGGCCGTGCC AAATGGGAAA GTCGTAACCT GGTGGCCCAA TGGCTACGGC 
GAGCAGAAAC TGTATCCGCT CCACTTCACT CTGAAGACCT GGCTGGGACC TGATGGACCC 
GAGGTTCGCT CTAAGACCAA GTCGCACAAG TCGCTCCGAG TGGGTTTTCG TACGCTTGAT 
CTAGTAGAGG ATCCCGCCCC TGACGGCGTT GGAAACACCT TCCTGTTTCG AGTTAATGGT 
GTAGAAATGT TTATGAAAGG CAGTAACTAC ATACCATCCC ACTTATTACC CGAAATGCAA 
ACAAATGAAC AGATTTCACA TTTATTAAGG TCCGCCAAGG AAGCCCATAT GAACATGCTT 
CGAGTCTGGG GAGGCGGAGT TTACGAATCG GACTACTTTT ACCAGCTCGC CGACAGCTTG 
GGTCTTCTAA TTTGGCAAGA TATGATGTTT GCTTGTGCCA TGTACCCGGT TAACGACGAA 
TTCCTATCGT CAGTGCGTGA GGAGGTTCGC CAGAATGCCA TGCGACTATC ACACCATCCA 
AGCGTAGCCA TTTTTGTGAC CAATAACGAA AACGAAGCTG CTTTGGTACA GAACTGGTAC 
GGCACGCTTT TCGAAAGAGA TCGATTTGAG AGCGAGTACC GGGAGCTCTA CCTTGCTAAT 
GTCATCCACG AGTTGAAGCT TGTTTCGCAT AGCTCTCGAC CTCAGCCACT GGTCTCCTCA 
CCTTCAAATG GCAAAGCTAG TGAACCGGAC AACTATATTT CGAGCAACCC TCAGGATAAC 
CAAAATGGCG ATGTACATTT CTACGACTAC ACTAAGGACG GCTGGGATCC AGGAATTTTC 
CCACGTCCTC GATTCGTCAG TGAGTTCGGC TTTCAGAGCT TTCCAGGCGC ATACGCATGG 
CAGCGCAGCA AGGGCGAGGA CGATGACCTC CTCGGCCTGA TCACACACCG ACAACACCAC 
CCACTGGGAA ATGTTCCTGT TATCGCACTA GTGGAGAGAC ACCTACCGCT GCCCTTTCCA 
GAGAATGAGA ACTACGCGAC AGCACTGATT TACTTCAGCC AGATTGCTCA AGCCATGGCC 
ACAAAGGTGG AAACCGAATT ATACCGTAGC CTTCGGGACA CTCCCCACAG AACGATGGGG 
GCCCTTTACT GGCAGCTGAA TGATGTATGG GTGGCGCCAT CCTGGTCCAG CATTGATTTC 
TACGGCAACT GGAAGCTGCT CCATTACTGG GCACGTGATT TTCTAGCGCC TATTTCAATC 
GTTGCTCTTT ACGAAAAGTC CACGGATTCG TTAAACATCT CCCTTATTTG TGACCAATTG 
GAGGTGGACC CAAGGGAACT GAATGTCGTA GCTAACGTTC ACCTGTGGTC CCAGCTTCTG 
CCTCGGCAGT CCACCTCCTG GGATGTTACC CTGCGCCCCA ATGGCGTGCA GTACGACAAG 
GTCATTGCCA TCTCCGATCT GCTGCAAGGA GAGTTCACCA GAACTAACGC GTTTTTGGAG 
CTGCAGTTGC GCCGCGGGCA GGATGTGATC TCTAGGACCC ACTTCTTTCC CAGTAGCATT 
GCCGGAGCAG TCGGAATAGA GGACCCCGGG CTTGAGTTCG AGATTGCCTC CCGTACCTGT 
CTTAACACCA CAAACGTCGT CCGCAACAGC GTCAGCATTA GCATACAGGT GAAGCGCCCC 
GCCGTATTTG TATACCTAGA GCTGCTCAAG CCATACCGCT ACACCCTGTC CGAGAACGGA 
TTTATGCAGA CTGAGCCAAT GCATGTCGTC TACCTAACGT TCGACGCACC CTTGTGCGCC 
CGCCTTTTGC GCAAGTCGGA CCTCCGTGTG CTGACCGTCA ACGACTTTAT GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262241.1
CDS	638..2974
Translation	MFVRYSYEVSSLLQAENFLEVEIQSPVVAALARANALKEAKKSVPPECPNERYHGECHMNMLRKMQASFSWDWGPAAPSAGIWKNVELEIYEVAVIREVDVDVSRVNGSYWNMHIRCFLDAGGWQNFNGRLVLYAVELLDRPVVVDRYAIKNISHLAPVIEFDQAVPNGKVVTWWPNGYGEQKLYPLHFTLKTWLGPDGPEVRSKTKSHKSLRVGFRTLDLVEDPAPDGVGNTFLFRVNGVEMFMKGSNYIPSHLLPEMQTNEQISHLLRSAKEAHMNMLRVWGGGVYESDYFYQLADSLGLLIWQDMMFACAMYPVNDEFLSSVREEVRQNAMRLSHHPSVAIFVTNNENEAALVQNWYGTLFERDRFESEYRELYLANVIHELKLVSHSSRPQPLVSSPSNGKASEPDNYISSNPQDNQNGDVHFYDYTKDGWDPGIFPRPRFVSEFGFQSFPGAYAWQRSKGEDDDLLGLITHRQHHPLGNVPVIALVERHLPLPFPENENYATALIYFSQIAQAMATKVETELYRSLRDTPHRTMGALYWQLNDVWVAPSWSSIDFYGNWKLLHYWARDFLAPISIVALYEKSTDSLNISLICDQLEVDPRELNVVANVHLWSQLLPRQSTSWDVTLRPNGVQYDKVIAISDLLQGEFTRTNAFLELQLRRGQDVISRTHFFPSSIAGAVGIEDPGLEFEIASRTCLNTTNVVRNSVSISIQVKRPAVFVYLELLKPYRYTLSENGFMQTEPMHVVYLTFDAPLCARLLRKSDLRVLTVNDFMR

Target ORF information:

RefSeq Version	NM_001275312.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275312.1

ORF Insert Sequence:

ATGTTTGTCC GCTACTCTTA CGAAGTGAGT TCCCTGCTCC AAGCGGAAAA CTTTCTGGAA 
GTTGAGATCC AATCTCCAGT TGTGGCTGCT TTGGCAAGAG CCAATGCTTT AAAAGAAGCT 
AAAAAGAGCG TGCCTCCCGA ATGCCCCAAC GAGCGCTACC ATGGTGAGTG CCACATGAAC 
ATGCTGCGGA AGATGCAGGC TAGCTTTTCC TGGGACTGGG GTCCTGCAGC TCCCTCTGCT 
GGAATCTGGA AAAACGTGGA GCTGGAAATC TACGAGGTGG CGGTTATTCG TGAGGTGGAT 
GTGGACGTTA GCCGCGTCAA TGGTTCGTAC TGGAACATGC ATATTCGCTG CTTCCTGGAC 
GCGGGAGGCT GGCAAAACTT CAATGGAAGA CTCGTCCTAT ATGCTGTCGA GCTATTGGAC 
CGTCCGGTAG TAGTAGACAG ATACGCAATA AAAAACATCA GTCACTTGGC GCCAGTAATT 
GAATTCGATC AGGCCGTGCC AAATGGGAAA GTCGTAACCT GGTGGCCCAA TGGCTACGGC 
GAGCAGAAAC TGTATCCGCT CCACTTCACT CTGAAGACCT GGCTGGGACC TGATGGACCC 
GAGGTTCGCT CTAAGACCAA GTCGCACAAG TCGCTCCGAG TGGGTTTTCG TACGCTTGAT 
CTAGTAGAGG ATCCCGCCCC TGACGGCGTT GGAAACACCT TCCTGTTTCG AGTTAATGGT 
GTAGAAATGT TTATGAAAGG CAGTAACTAC ATACCATCCC ACTTATTACC CGAAATGCAA 
ACAAATGAAC AGATTTCACA TTTATTAAGG TCCGCCAAGG AAGCCCATAT GAACATGCTT 
CGAGTCTGGG GAGGCGGAGT TTACGAATCG GACTACTTTT ACCAGCTCGC CGACAGCTTG 
GGTCTTCTAA TTTGGCAAGA TATGATGTTT GCTTGTGCCA TGTACCCGGT TAACGACGAA 
TTCCTATCGT CAGTGCGTGA GGAGGTTCGC CAGAATGCCA TGCGACTATC ACACCATCCA 
AGCGTAGCCA TTTTTGTGAC CAATAACGAA AACGAAGCTG CTTTGGTACA GAACTGGTAC 
GGCACGCTTT TCGAAAGAGA TCGATTTGAG AGCGAGTACC GGGAGCTCTA CCTTGCTAAT 
GTCATCCACG AGTTGAAGCT TGTTTCGCAT AGCTCTCGAC CTCAGCCACT GGTCTCCTCA 
CCTTCAAATG GCAAAGCTAG TGAACCGGAC AACTATATTT CGAGCAACCC TCAGGATAAC 
CAAAATGGCG ATGTACATTT CTACGACTAC ACTAAGGACG GCTGGGATCC AGGAATTTTC 
CCACGTCCTC GATTCGTCAG TGAGTTCGGC TTTCAGAGCT TTCCAGGCGC ATACGCATGG 
CAGCGCAGCA AGGGCGAGGA CGATGACCTC CTCGGCCTGA TCACACACCG ACAACACCAC 
CCACTGGGAA ATGTTCCTGT TATCGCACTA GTGGAGAGAC ACCTACCGCT GCCCTTTCCA 
GAGAATGAGA ACTACGCGAC AGCACTGATT TACTTCAGCC AGATTGCTCA AGCCATGGCC 
ACAAAGGTGG AAACCGAATT ATACCGTAGC CTTCGGGACA CTCCCCACAG AACGATGGGG 
GCCCTTTACT GGCAGCTGAA TGATGTATGG GTGGCGCCAT CCTGGTCCAG CATTGATTTC 
TACGGCAACT GGAAGCTGCT CCATTACTGG GCACGTGATT TTCTAGCGCC TATTTCAATC 
GTTGCTCTTT ACGAAAAGTC CACGGATTCG TTAAACATCT CCCTTATTTG TGACCAATTG 
GAGGTGGACC CAAGGGAACT GAATGTCGTA GCTAACGTTC ACCTGTGGTC CCAGCTTCTG 
CCTCGGCAGT CCACCTCCTG GGATGTTACC CTGCGCCCCA ATGGCGTGCA GTACGACAAG 
GTCATTGCCA TCTCCGATCT GCTGCAAGGA GAGTTCACCA GAACTAACGC GTTTTTGGAG 
CTGCAGTTGC GCCGCGGGCA GGATGTGATC TCTAGGACCC ACTTCTTTCC CAGTAGCATT 
GCCGGAGCAG TCGGAATAGA GGACCCCGGG CTTGAGTTCG AGATTGCCTC CCGTACCTGT 
CTTAACACCA CAAACGTCGT CCGCAACAGC GTCAGCATTA GCATACAGGT GAAGCGCCCC 
GCCGTATTTG TATACCTAGA GCTGCTCAAG CCATACCGCT ACACCCTGTC CGAGAACGGA 
TTTATGCAGA CTGAGCCAAT GCATGTCGTC TACCTAACGT TCGACGCACC CTTGTGCGCC 
CGCCTTTTGC GCAAGTCGGA CCTCCGTGTG CTGACCGTCA ACGACTTTAT GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

beta-Man ( NM_001275313.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262242.1 Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant H, mRNA.

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CloneID	OFa07848
Clone ID Related Accession (Same CDS sequence)	NM_001275313.1
Accession Version	NM_001275313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta-Mannosidase, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTACG GATGCGGATT ACTCAGGAAT GGCACCACGG GCGCTGTTAT GGTCGGATTA 
GTTTGTGGCC TTTATGTTTG CAGCGGCCAA TGTGATAAGG TGGAAGTAAT AGAGCTGAGC 
AATTGGACGA TAACTAATCA GAATGGCTCC CTGACTATTG CCAACCAAAC GTTGCCCTTG 
GGCATATACA GTGCCTTCGC ACCCCAAGTG CTGGACTCCT ACAACGACGT CGGTCTGCGT 
TACTTGGCCT ACGACAACTT TACCTTCTCC AATTTCTTTC AGTTCGACGT TCATCACTTC 
AATCGCGGCC ACATCAACCT GACGTTTCAC GGCATTGACA CGGTGTCGGA AATCCGGCTG 
AACCATCAAC TGCTGGGACG CACGGACAAC ATGTTTGTCC GCTACTCTTA CGAAGTGAGT 
TCCCTGCTCC AAGCGGAAAA CTTTCTGGAA GTTGAGATCC AATCTCCAGT TGTGGCTGCT 
TTGGCAAGAG CCAATGCTTT AAAAGAAGCT AAAAAGAGCG TGCCTCCCGA ATGCCCCAAC 
GAGCGCTACC ATGGTGAGTG CCACATGAAC ATGCTGCGGA AGATGCAGGC TAGCTTTTCC 
TGGGACTGGG GTCCTGCAGC TCCCTCTGCT GGAATCTGGA AAAACGTGGA GCTGGAAATC 
TACGAGGTGG CGGTTATTCG TGAGGTGGAT GTGGACGTTA GCCGCGTCAA TGGTTCGTAC 
TGGAACATGC ATATTCGCTG CTTCCTGGAC GCGGGAGGCT GGCAAAACTT CAATGGAAGA 
CTCGTCCTAT ATGCTGTCGA GCTATTGGAC CGTCCGGTAG TAGTAGACAG ATACGCAATA 
AAAAACATCA GTCACTTGGC GCCAGTAATT GAATTCGATC AGGCCGTGCC AAATGGGAAA 
GTCGTAACCT GGTGGCCCAA TGGCTACGGC GAGCAGAAAC TGTATCCGCT CCACTTCACT 
CTGAAGACCT GGCTGGGACC TGATGGACCC GAGGTTCGCT CTAAGACCAA GTCGCACAAG 
TCGCTCCGAG TGGGTTTTCG TACGCTTGAT CTAGTAGAGG ATCCCGCCCC TGACGGCGTT 
GGAAACACCT TCCTGTTTCG AGTTAATGGT GTAGAAATGT TTATGAAAGG CAGTAACTAC 
ATACCATCCC ACTTATTACC CGAAATGCAA ACAAATGAAC AGATTTCACA TTTATTAAGG 
TCCGCCAAGG AAGCCCATAT GAACATGCTT CGAGTCTGGG GAGGCGGAGT TTACGAATCG 
GACTACTTTT ACCAGCTCGC CGACAGCTTG GGTCTTCTAA TTTGGCAAGA TATGATGTTT 
GCTTGTGCCA TGTACCCGGT TAACGACGAA TTCCTATCGT CAGTGCGTGA GGAGGTTCGC 
CAGAATGCCA TGCGACTATC ACACCATCCA AGCGTAGCCA TTTTTGTGAC CAATAACGAA 
AACGAAGCTG CTTTGGTACA GAACTGGTAC GGCACGCTTT TCGAAAGAGA TCGATTTGAG 
AGCGAGTACC GGGAGCTCTA CCTTGCTAAT GTCATCCACG AGTTGAAGCT TGTTTCGCAT 
AGCTCTCGAC CTCAGCCACT GGTCTCCTCA CCTTCAAATG GCAAAGCTAG TGAACCGGAC 
AACTATATTT CGAGCAACCC TCAGGATAAC CAAAATGGCG ATGTACATTT CTACGACTAC 
ACTAAGGACG GCTGGGATCC AGGAATTTTC CCACGTCCTC GATTCGTCAG TGAGTTCGGC 
TTTCAGAGCT TTCCAGGCGC ATACGCATGG CAGCGCAGCA AGGGCGAGGA CGATGACCTC 
CTCGGCCTGA TCACACACCG ACAACACCAC CCACTGGGAA ATGTTCCTGT TATCGCACTA 
GTGGAGAGAC ACCTACCGCT GCCCTTTCCA GAGAATGAGA ACTACGCGAC AGCACTGATT 
TACTTCAGCC AGATTGCTCA AGCCATGGCC ACAAAGGTGG AAACCGAATT ATACCGTAGC 
CTTCGGGACA CTCCCCACAG AACGATGGGG GCCCTTTACT GGCAGCTGAA TGATGTATGG 
GTGGCGCCAT CCTGGTCCAG CATTGATTTC TACGGCAACT GGAAGCTGCT CCATTACTGG 
GCACGTGATT TTCTAGCGCC TATTTCAATC GTTGCTCTTT ACGAAAAGTC CACGGATTCG 
TTAAACATCT CCCTTATTTG TGACCAATTG GAGGTGGACC CAAGGGAACT GAATGTCGTA 
GCTAACGTTC ACCTGTGGTC CCAGCTTCTG CCTCGGCAGT CCACCTCCTG GGATGTTACC 
CTGCGCCCCA ATGGCGTGCA GTACGACAAG GTCATTGCCA TCTCCGATCT GCTGCAAGGA 
GAGTTCACCA GAACTAACGC GTTTTTGGAG CTGCAGTTGC GCCGCGGGCA GGATGTGATC 
TCTAGGACCC ACTTCTTTCC CAGTAGCATT GCCGGAGCAG TCGGAATAGA GGACCCCGGG 
CTTGAGATTG CCTCCCGTAC CTGTCTTAAC ACCACAAACG TCGTCCGCAA CAGCGTCAGC 
ATTAGCATAC AGGTGAAGCG CCCCGCCGTA TTTGTATACC TAGAGCTGCT CAAGCCATAC 
CGCTACACCC TGTCCGAGAA CGGATTTATG CAGACTGAGC CAATGCATGT CGTCTACCTA 
ACGTTCGACG CACCCTTGTG CGCCCGCCTT TTGCGCAAGT CGGACCTCCG TGTGCTGACC 
GTCAACGACT TTATGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262242.1
CDS	210..2930
Translation	MKYGCGLLRNGTTGAVMVGLVCGLYVCSGQCDKVEVIELSNWTITNQNGSLTIANQTLPLGIYSAFAPQVLDSYNDVGLRYLAYDNFTFSNFFQFDVHHFNRGHINLTFHGIDTVSEIRLNHQLLGRTDNMFVRYSYEVSSLLQAENFLEVEIQSPVVAALARANALKEAKKSVPPECPNERYHGECHMNMLRKMQASFSWDWGPAAPSAGIWKNVELEIYEVAVIREVDVDVSRVNGSYWNMHIRCFLDAGGWQNFNGRLVLYAVELLDRPVVVDRYAIKNISHLAPVIEFDQAVPNGKVVTWWPNGYGEQKLYPLHFTLKTWLGPDGPEVRSKTKSHKSLRVGFRTLDLVEDPAPDGVGNTFLFRVNGVEMFMKGSNYIPSHLLPEMQTNEQISHLLRSAKEAHMNMLRVWGGGVYESDYFYQLADSLGLLIWQDMMFACAMYPVNDEFLSSVREEVRQNAMRLSHHPSVAIFVTNNENEAALVQNWYGTLFERDRFESEYRELYLANVIHELKLVSHSSRPQPLVSSPSNGKASEPDNYISSNPQDNQNGDVHFYDYTKDGWDPGIFPRPRFVSEFGFQSFPGAYAWQRSKGEDDDLLGLITHRQHHPLGNVPVIALVERHLPLPFPENENYATALIYFSQIAQAMATKVETELYRSLRDTPHRTMGALYWQLNDVWVAPSWSSIDFYGNWKLLHYWARDFLAPISIVALYEKSTDSLNISLICDQLEVDPRELNVVANVHLWSQLLPRQSTSWDVTLRPNGVQYDKVIAISDLLQGEFTRTNAFLELQLRRGQDVISRTHFFPSSIAGAVGIEDPGLEIASRTCLNTTNVVRNSVSISIQVKRPAVFVYLELLKPYRYTLSENGFMQTEPMHVVYLTFDAPLCARLLRKSDLRVLTVNDFMR

Target ORF information:

RefSeq Version	NM_001275313.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta-Mannosidase (beta-Man), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275313.1

ORF Insert Sequence:

ATGAAGTACG GATGCGGATT ACTCAGGAAT GGCACCACGG GCGCTGTTAT GGTCGGATTA 
GTTTGTGGCC TTTATGTTTG CAGCGGCCAA TGTGATAAGG TGGAAGTAAT AGAGCTGAGC 
AATTGGACGA TAACTAATCA GAATGGCTCC CTGACTATTG CCAACCAAAC GTTGCCCTTG 
GGCATATACA GTGCCTTCGC ACCCCAAGTG CTGGACTCCT ACAACGACGT CGGTCTGCGT 
TACTTGGCCT ACGACAACTT TACCTTCTCC AATTTCTTTC AGTTCGACGT TCATCACTTC 
AATCGCGGCC ACATCAACCT GACGTTTCAC GGCATTGACA CGGTGTCGGA AATCCGGCTG 
AACCATCAAC TGCTGGGACG CACGGACAAC ATGTTTGTCC GCTACTCTTA CGAAGTGAGT 
TCCCTGCTCC AAGCGGAAAA CTTTCTGGAA GTTGAGATCC AATCTCCAGT TGTGGCTGCT 
TTGGCAAGAG CCAATGCTTT AAAAGAAGCT AAAAAGAGCG TGCCTCCCGA ATGCCCCAAC 
GAGCGCTACC ATGGTGAGTG CCACATGAAC ATGCTGCGGA AGATGCAGGC TAGCTTTTCC 
TGGGACTGGG GTCCTGCAGC TCCCTCTGCT GGAATCTGGA AAAACGTGGA GCTGGAAATC 
TACGAGGTGG CGGTTATTCG TGAGGTGGAT GTGGACGTTA GCCGCGTCAA TGGTTCGTAC 
TGGAACATGC ATATTCGCTG CTTCCTGGAC GCGGGAGGCT GGCAAAACTT CAATGGAAGA 
CTCGTCCTAT ATGCTGTCGA GCTATTGGAC CGTCCGGTAG TAGTAGACAG ATACGCAATA 
AAAAACATCA GTCACTTGGC GCCAGTAATT GAATTCGATC AGGCCGTGCC AAATGGGAAA 
GTCGTAACCT GGTGGCCCAA TGGCTACGGC GAGCAGAAAC TGTATCCGCT CCACTTCACT 
CTGAAGACCT GGCTGGGACC TGATGGACCC GAGGTTCGCT CTAAGACCAA GTCGCACAAG 
TCGCTCCGAG TGGGTTTTCG TACGCTTGAT CTAGTAGAGG ATCCCGCCCC TGACGGCGTT 
GGAAACACCT TCCTGTTTCG AGTTAATGGT GTAGAAATGT TTATGAAAGG CAGTAACTAC 
ATACCATCCC ACTTATTACC CGAAATGCAA ACAAATGAAC AGATTTCACA TTTATTAAGG 
TCCGCCAAGG AAGCCCATAT GAACATGCTT CGAGTCTGGG GAGGCGGAGT TTACGAATCG 
GACTACTTTT ACCAGCTCGC CGACAGCTTG GGTCTTCTAA TTTGGCAAGA TATGATGTTT 
GCTTGTGCCA TGTACCCGGT TAACGACGAA TTCCTATCGT CAGTGCGTGA GGAGGTTCGC 
CAGAATGCCA TGCGACTATC ACACCATCCA AGCGTAGCCA TTTTTGTGAC CAATAACGAA 
AACGAAGCTG CTTTGGTACA GAACTGGTAC GGCACGCTTT TCGAAAGAGA TCGATTTGAG 
AGCGAGTACC GGGAGCTCTA CCTTGCTAAT GTCATCCACG AGTTGAAGCT TGTTTCGCAT 
AGCTCTCGAC CTCAGCCACT GGTCTCCTCA CCTTCAAATG GCAAAGCTAG TGAACCGGAC 
AACTATATTT CGAGCAACCC TCAGGATAAC CAAAATGGCG ATGTACATTT CTACGACTAC 
ACTAAGGACG GCTGGGATCC AGGAATTTTC CCACGTCCTC GATTCGTCAG TGAGTTCGGC 
TTTCAGAGCT TTCCAGGCGC ATACGCATGG CAGCGCAGCA AGGGCGAGGA CGATGACCTC 
CTCGGCCTGA TCACACACCG ACAACACCAC CCACTGGGAA ATGTTCCTGT TATCGCACTA 
GTGGAGAGAC ACCTACCGCT GCCCTTTCCA GAGAATGAGA ACTACGCGAC AGCACTGATT 
TACTTCAGCC AGATTGCTCA AGCCATGGCC ACAAAGGTGG AAACCGAATT ATACCGTAGC 
CTTCGGGACA CTCCCCACAG AACGATGGGG GCCCTTTACT GGCAGCTGAA TGATGTATGG 
GTGGCGCCAT CCTGGTCCAG CATTGATTTC TACGGCAACT GGAAGCTGCT CCATTACTGG 
GCACGTGATT TTCTAGCGCC TATTTCAATC GTTGCTCTTT ACGAAAAGTC CACGGATTCG 
TTAAACATCT CCCTTATTTG TGACCAATTG GAGGTGGACC CAAGGGAACT GAATGTCGTA 
GCTAACGTTC ACCTGTGGTC CCAGCTTCTG CCTCGGCAGT CCACCTCCTG GGATGTTACC 
CTGCGCCCCA ATGGCGTGCA GTACGACAAG GTCATTGCCA TCTCCGATCT GCTGCAAGGA 
GAGTTCACCA GAACTAACGC GTTTTTGGAG CTGCAGTTGC GCCGCGGGCA GGATGTGATC 
TCTAGGACCC ACTTCTTTCC CAGTAGCATT GCCGGAGCAG TCGGAATAGA GGACCCCGGG 
CTTGAGATTG CCTCCCGTAC CTGTCTTAAC ACCACAAACG TCGTCCGCAA CAGCGTCAGC 
ATTAGCATAC AGGTGAAGCG CCCCGCCGTA TTTGTATACC TAGAGCTGCT CAAGCCATAC 
CGCTACACCC TGTCCGAGAA CGGATTTATG CAGACTGAGC CAATGCATGT CGTCTACCTA 
ACGTTCGACG CACCCTTGTG CGCCCGCCTT TTGCGCAAGT CGGACCTCCG TGTGCTGACC 
GTCAACGACT TTATGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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beta-Man cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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