alpha-Cat cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	alpha-Cat
Entrez Gene ID	40517
Full Name	alpha Catenin
Synonyms	CG17947,CT39986,Dalpha-Cat,Dalpha-cat,DalphaC,Dalphacat,Dmcatp,Dmel\CG17947,LD30423p,a-Cat,a-cat,alpha-CAT,alpha-cat,alpha-catenin,alpha-ctn,alphaCAT,alphaCat,alphacat
General protein information	Preferred Names alpha Catenin Names CG17947 gene product from transcript CG17947-RA CG17947-PA CG17947-PB D-alpha-catenin Dalpha-Catenin Dalpha-cadherin a-catenin alpha catenin alpha-Cat-PA alpha-Cat-PB alpha-Catenin alphaCatenin dalphacatenin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 80F1-80F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300225.1	NP_001287154.1	alpha catenin, isoform B
NM_079495.3	NP_524219.1	alpha catenin, isoform A

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CTNNA2	NP_990467.1
Drosophila melanogaster (fruit fly)	alpha-Cat	NP_524219.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003424	XP_311710.4
Xenopus tropicalis (tropical clawed frog)	ctnna2	NP_001090774.1
Pan troglodytes (chimpanzee)	CTNNA2	XP_003309122.1
Rattus norvegicus (Norway rat)	Ctnna2	NP_001100068.1
Danio rerio (zebrafish)	ctnna2	NP_001152844.1
Homo sapiens (human)	CTNNA2	NP_001269526.1
	CTNNA2	XP_001112948.1
Canis lupus familiaris (dog)	CTNNA2	XP_005630556.1
Mus musculus (house mouse)	Ctnna2	NP_001103234.1
Caenorhabditis elegans (roundworm)	hmp-1	NP_001122991.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

alpha-Catenin controls actomyosin dynamics by stabilising and promoting the formation of actomyosin foci, and also stabilises DE-Cadherin (Drosophila E-Cadherin, also known as Shotgun) at the cell membrane, suggesting that medioapical actomyosin contractility regulates junction stability.
Title: ?-Catenin stabilises Cadherin-Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction.

Increased Rap1 activity restricts epithelial invagination in an alpha-catenin-dependent manner.
Title: Distinct Rap1 activity states control the extent of epithelial invagination via ?-catenin.

alpha-Cat mutant phenotype can be rescued by the expression of a DE-cadherin::alpha-Catenin fusion protein, which argues against an essential cytosolic, cadherin-independent role of Drosophila alpha-Catenin
Title: Mutational analysis supports a core role for Drosophila ?-catenin in adherens junction function.

alpha-catenin and p120(ctn) are key players in a mechanism of recruiting Rho1 to its sites of action.
Title: Rho1 interacts with p120ctn and alpha-catenin, and regulates cadherin-based adherens junction components in Drosophila.

The following alpha-Cat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alpha-Cat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa22040	NM_001300225.1 Latest version!	Drosophila melanogaster alpha catenin, transcript variant B (alpha-Cat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19319	NM_079495.3 Latest version!	Drosophila melanogaster alpha catenin, transcript variant A (alpha-Cat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alpha-Cat ( NM_001300225.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287154.1 Drosophila melanogaster alpha catenin, transcript variant B (alpha-Cat), mRNA.

Price & Availability↑

CloneID	OFa22040
Clone ID Related Accession (Same CDS sequence)	NM_001300225.1
Accession Version	NM_001300225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha catenin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAAAAC CTGATAAAAT GGGCACGTTA ACCGATTTCG GACAGATAGC TTTGAAATGG 
GATCCCAAAA ATTTGGAAAT TCGCACAATG TCAGTTGAAA AAACACTTGA ACCCCTTGTA 
TTACAAGTAA CTACTCTTGT AAATACCAAG GGCCCAAGCA AAAAGAAAAA AGGAAAATCA 
AAGCGGGCCA GCGCATTAGT TGCAGCTGTT GAAAAAGCTA CAGAAAATTT TATTCAAAAA 
GGTGAACAGA TCGCTTACGA GAACCCAGAC ATTACACAAG AAATGTTAAC AGCTGTGGAT 
GAAGTAAAAA AAACTGGAGA TGCTATGAGC ATTGCAGCCA GAGAATTTTC TGAAGATCCG 
TGCAGTTCCC TGAAGAGAGG AAATATGGTG CGCGCAGCTA GGAATCTGTT GTCAGCTGTA 
ACCCGCTTGC TGATTTTAGC TGATATGGTT GATGTACATT TGCTCTTAAA ATCACTCCAC 
ATTGTCGAAG ATGATCTAAA CAAACTCAAA AACGCTTCGA GTCAGGACGA GCTTATGGAT 
AATATGAGGC AATTCGGACG CAATGCAGGA GAACTTATAA AACAGGCAGC CAAACGTCAG 
CAAGAACTTA AGGATCCCCA ATTAAGGGAC GATTTAGCAG CTGCTCGGGC GATGCTTAAA 
AAACATTCAA CTATGCTGTT AACTGCATCA AAAGTATACG TTCGTCATCC GGAACTAGAT 
CTAGCAAAAG TAAATCGCGA TTTCATTCTA AAACAAGTTT GCGATGCTGT AAATACTATT 
AGCGATGTTG CCCAAGGAAA GTCATCCCAA CCGACAGATA TATACAGTGG AGCGGGAGAG 
CTGGCTGCAG CATTAGACGA CTTTGACGAA GGAATTGTTA TGGATCCCAT GACCTACAGC 
GAAAAGCGTT CACGTCAATT GCTCGAAGAG CGTCTGGAAA GTATTATTAG TGCAGCTGCA 
TTGATGGCGG ATGCAGATTG TACTCGAGAC GAGAGACGAG AGAGAATTGT GGCCGAATGC 
AATGCTGTGC GACAGGCTTT ACAGGATTTG TTATCTGAAT ATATGTCAAA CGTGAGTAAC 
ATACTTATAA TTATTATTTT TATACCCGTT ACTCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287154.1
CDS	55..1173
Translation	MLKPDKMGTLTDFGQIALKWDPKNLEIRTMSVEKTLEPLVLQVTTLVNTKGPSKKKKGKSKRASALVAAVEKATENFIQKGEQIAYENPDITQEMLTAVDEVKKTGDAMSIAAREFSEDPCSSLKRGNMVRAARNLLSAVTRLLILADMVDVHLLLKSLHIVEDDLNKLKNASSQDELMDNMRQFGRNAGELIKQAAKRQQELKDPQLRDDLAAARAMLKKHSTMLLTASKVYVRHPELDLAKVNRDFILKQVCDAVNTISDVAQGKSSQPTDIYSGAGELAAALDDFDEGIVMDPMTYSEKRSRQLLEERLESIISAAALMADADCTRDERRERIVAECNAVRQALQDLLSEYMSNVSNILIIIIFIPVTR

Target ORF information:

RefSeq Version	NM_001300225.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha catenin, transcript variant B (alpha-Cat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300225.1

ORF Insert Sequence:

ATGTTAAAAC CTGATAAAAT GGGCACGTTA ACCGATTTCG GACAGATAGC TTTGAAATGG 
GATCCCAAAA ATTTGGAAAT TCGCACAATG TCAGTTGAAA AAACACTTGA ACCCCTTGTA 
TTACAAGTAA CTACTCTTGT AAATACCAAG GGCCCAAGCA AAAAGAAAAA AGGAAAATCA 
AAGCGGGCCA GCGCATTAGT TGCAGCTGTT GAAAAAGCTA CAGAAAATTT TATTCAAAAA 
GGTGAACAGA TCGCTTACGA GAACCCAGAC ATTACACAAG AAATGTTAAC AGCTGTGGAT 
GAAGTAAAAA AAACTGGAGA TGCTATGAGC ATTGCAGCCA GAGAATTTTC TGAAGATCCG 
TGCAGTTCCC TGAAGAGAGG AAATATGGTG CGCGCAGCTA GGAATCTGTT GTCAGCTGTA 
ACCCGCTTGC TGATTTTAGC TGATATGGTT GATGTACATT TGCTCTTAAA ATCACTCCAC 
ATTGTCGAAG ATGATCTAAA CAAACTCAAA AACGCTTCGA GTCAGGACGA GCTTATGGAT 
AATATGAGGC AATTCGGACG CAATGCAGGA GAACTTATAA AACAGGCAGC CAAACGTCAG 
CAAGAACTTA AGGATCCCCA ATTAAGGGAC GATTTAGCAG CTGCTCGGGC GATGCTTAAA 
AAACATTCAA CTATGCTGTT AACTGCATCA AAAGTATACG TTCGTCATCC GGAACTAGAT 
CTAGCAAAAG TAAATCGCGA TTTCATTCTA AAACAAGTTT GCGATGCTGT AAATACTATT 
AGCGATGTTG CCCAAGGAAA GTCATCCCAA CCGACAGATA TATACAGTGG AGCGGGAGAG 
CTGGCTGCAG CATTAGACGA CTTTGACGAA GGAATTGTTA TGGATCCCAT GACCTACAGC 
GAAAAGCGTT CACGTCAATT GCTCGAAGAG CGTCTGGAAA GTATTATTAG TGCAGCTGCA 
TTGATGGCGG ATGCAGATTG TACTCGAGAC GAGAGACGAG AGAGAATTGT GGCCGAATGC 
AATGCTGTGC GACAGGCTTT ACAGGATTTG TTATCTGAAT ATATGTCAAA CGTGAGTAAC 
ATACTTATAA TTATTATTTT TATACCCGTT ACTCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alpha-Cat ( NM_079495.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524219.1 Drosophila melanogaster alpha catenin, transcript variant A (alpha-Cat), mRNA.

Price & Availability↑

CloneID	OFa19319
Clone ID Related Accession (Same CDS sequence)	NM_079495.3
Accession Version	NM_079495.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha catenin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079495.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAAAAC CTGATAAAAT GGGCACGTTA ACCGATTTCG GACAGATAGC TTTGAAATGG 
GATCCCAAAA ATTTGGAAAT TCGCACAATG TCAGTTGAAA AAACACTTGA ACCCCTTGTA 
TTACAAGTAA CTACTCTTGT AAATACCAAG GGCCCAAGCA AAAAGAAAAA AGGAAAATCA 
AAGCGGGCCA GCGCATTAGT TGCAGCTGTT GAAAAAGCTA CAGAAAATTT TATTCAAAAA 
GGTGAACAGA TCGCTTACGA GAACCCAGAC ATTACACAAG AAATGTTAAC AGCTGTGGAT 
GAAGTAAAAA AAACTGGAGA TGCTATGAGC ATTGCAGCCA GAGAATTTTC TGAAGATCCG 
TGCAGTTCCC TGAAGAGAGG AAATATGGTG CGCGCAGCTA GGAATCTGTT GTCAGCTGTA 
ACCCGCTTGC TGATTTTAGC TGATATGGTT GATGTACATT TGCTCTTAAA ATCACTCCAC 
ATTGTCGAAG ATGATCTAAA CAAACTCAAA AACGCTTCGA GTCAGGACGA GCTTATGGAT 
AATATGAGGC AATTCGGACG CAATGCAGGA GAACTTATAA AACAGGCAGC CAAACGTCAG 
CAAGAACTTA AGGATCCCCA ATTAAGGGAC GATTTAGCAG CTGCTCGGGC GATGCTTAAA 
AAACATTCAA CTATGCTGTT AACTGCATCA AAAGTATACG TTCGTCATCC GGAACTAGAT 
CTAGCAAAAG TAAATCGCGA TTTCATTCTA AAACAAGTTT GCGATGCTGT AAATACTATT 
AGCGATGTTG CCCAAGGAAA GTCATCCCAA CCGACAGATA TATACAGTGG AGCGGGAGAG 
CTGGCTGCAG CATTAGACGA CTTTGACGAA GGAATTGTTA TGGATCCCAT GACCTACAGC 
GAAAAGCGTT CACGTCAATT GCTCGAAGAG CGTCTGGAAA GTATTATTAG TGCAGCTGCA 
TTGATGGCGG ATGCAGATTG TACTCGAGAC GAGAGACGAG AGAGAATTGT GGCCGAATGC 
AATGCTGTGC GACAGGCTTT ACAGGATTTG TTATCTGAAT ATATGTCAAA CATGAGTCAA 
AAAGATAACA GCCCAGGACT CTCTCGAGCG ATTGATCAAA TGTGTCGAAA GACTCGCGAC 
CTAAGAAGGC AATTGCGAAA GGCTGTTGTG GATCATGTTT CGGACTCTTT TTTGGAGACT 
ACAACTCCTT TGCTAGATTT AATTGAAGCT GCAAAATCGG GTAATGAAAA AAAAGTTCGG 
GAAAAGTCCG AAATATTCAC CAAGCACGCT GAAAAACTAG TTGAAGTAGC AAATTTAGTA 
TGTAGCATGT CAAACAATGA AGATGGTGTT AAAATGGTTC GATATGCTGC TGCTCAAATT 
GAAAGTCTTT GTCCGCAAGT AATAAATGCA GCATCGATAT TGACTGTTAG ACCGAATTCA 
AAAGTAGCTC AAGAAAATAT GACTACTTAT CGACAAGCCT GGGAGGTGCA AGTTCGTATT 
TTAACCGAGG CAGTGGATGA TATTACAACA ATTGACGACT TTTTAGCAGT ATCTGAGAAT 
CACATTCTTG AAGATGTAAA CAAATGTGTA ATGGCTTTAC AAGTTGGTGA TGCCAGGGAT 
TTGCGTGCAA CTGCTGGTGC TATTCAAGGT CGATCATCAC GAGTTTGTAA TGTTGTTGAA 
GCTGAAATGG ATAATTATGA ACCATGTATT TACACCAAAC GAGTCTTAGA AGCAGTCAAA 
GTTCTACGAG ATCAAGTTAT GATGAAATTT GACCAACGTG TAGGAGCAGC CGTTGGAGCC 
CTTTCAAATA ACTCCAATAA GGATGTTGAC GAAAATGACT TCATTGATGC TTCTCGTTTG 
GTTTACGACG GAGTTCGTGA AATTCGAAGA GCTGTTTTAA TGAATCGAAG CTCGGAAGAC 
CTTGATACAG ATACTGAATT TGAGCCAGTT GAAGACTTAA CCTTGGAAAC TCGAAGTCGA 
TCAAGTGCTC ATACCGGCGA TCAAACCGTT GACGAATATC CCGATATAAG TGGCATATGT 
ACAGCTCGAG AAGCAATGCG AAAAATGACG GAAGAAGACA AACAAAAAAT TGCTCAGCAA 
GTGGAGTTAT TCCGTAGGGA AAAACTAACT TTTGATTCAG AAGTTGCTAA ATGGGATGAC 
ACTGGAAATG ACATTATTTT TCTGGCCAAA CATATGTGCA TGATTATGAT GGAAATGACG 
GATTTTACCA GAGGACGAGG ACCTTTAAAA ACTACTATGG ATGTTATTAA CGCAGCTAAA 
AAAATTTCGG AAGCTGGTAC AAAGCTGGAT AAACTAACCA GGGAAATAGC AGAGCAATGC 
CCAGAAAGCA GCACGAAAAA GGACCTTTTA GCGTATTTGC AACGCATTGC CCTGTATTGT 
CATCAAATCC AAATAACTTC AAAAGTAAAA GCAGACGTTC AAAATATAAG CGGCGAACTT 
ATAGTTTCTG GGCTGGACAG CGCTACATCG TTAATTCAAG CTGCTAAAAA TTTAATGAAT 
GCCGTTGTAT TAACGGTAAA GTACTCATAT GTGGCATCAA CAAAATATAC CAGGCAAGGA 
ACAGTGTCTT CTCCAATTGT TGTATGGAAG ATGAAAGCAC CAGAAAAAAA ACCATTGGTC 
AGGCCTGAAA AACCAGAAGA AGTACGGGCC AAAGTTCGCA AGGGATCTCA AAAAAAGGTT 
CAAAACCCTA TACATGCATT ATCTGAATTC CAGAGTCCTG CTGACGCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524219.1
CDS	55..2808
Translation	MLKPDKMGTLTDFGQIALKWDPKNLEIRTMSVEKTLEPLVLQVTTLVNTKGPSKKKKGKSKRASALVAAVEKATENFIQKGEQIAYENPDITQEMLTAVDEVKKTGDAMSIAAREFSEDPCSSLKRGNMVRAARNLLSAVTRLLILADMVDVHLLLKSLHIVEDDLNKLKNASSQDELMDNMRQFGRNAGELIKQAAKRQQELKDPQLRDDLAAARAMLKKHSTMLLTASKVYVRHPELDLAKVNRDFILKQVCDAVNTISDVAQGKSSQPTDIYSGAGELAAALDDFDEGIVMDPMTYSEKRSRQLLEERLESIISAAALMADADCTRDERRERIVAECNAVRQALQDLLSEYMSNMSQKDNSPGLSRAIDQMCRKTRDLRRQLRKAVVDHVSDSFLETTTPLLDLIEAAKSGNEKKVREKSEIFTKHAEKLVEVANLVCSMSNNEDGVKMVRYAAAQIESLCPQVINAASILTVRPNSKVAQENMTTYRQAWEVQVRILTEAVDDITTIDDFLAVSENHILEDVNKCVMALQVGDARDLRATAGAIQGRSSRVCNVVEAEMDNYEPCIYTKRVLEAVKVLRDQVMMKFDQRVGAAVGALSNNSNKDVDENDFIDASRLVYDGVREIRRAVLMNRSSEDLDTDTEFEPVEDLTLETRSRSSAHTGDQTVDEYPDISGICTAREAMRKMTEEDKQKIAQQVELFRREKLTFDSEVAKWDDTGNDIIFLAKHMCMIMMEMTDFTRGRGPLKTTMDVINAAKKISEAGTKLDKLTREIAEQCPESSTKKDLLAYLQRIALYCHQIQITSKVKADVQNISGELIVSGLDSATSLIQAAKNLMNAVVLTVKYSYVASTKYTRQGTVSSPIVVWKMKAPEKKPLVRPEKPEEVRAKVRKGSQKKVQNPIHALSEFQSPADAV

Target ORF information:

RefSeq Version	NM_079495.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha catenin, transcript variant A (alpha-Cat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079495.3

ORF Insert Sequence:

ATGTTAAAAC CTGATAAAAT GGGCACGTTA ACCGATTTCG GACAGATAGC TTTGAAATGG 
GATCCCAAAA ATTTGGAAAT TCGCACAATG TCAGTTGAAA AAACACTTGA ACCCCTTGTA 
TTACAAGTAA CTACTCTTGT AAATACCAAG GGCCCAAGCA AAAAGAAAAA AGGAAAATCA 
AAGCGGGCCA GCGCATTAGT TGCAGCTGTT GAAAAAGCTA CAGAAAATTT TATTCAAAAA 
GGTGAACAGA TCGCTTACGA GAACCCAGAC ATTACACAAG AAATGTTAAC AGCTGTGGAT 
GAAGTAAAAA AAACTGGAGA TGCTATGAGC ATTGCAGCCA GAGAATTTTC TGAAGATCCG 
TGCAGTTCCC TGAAGAGAGG AAATATGGTG CGCGCAGCTA GGAATCTGTT GTCAGCTGTA 
ACCCGCTTGC TGATTTTAGC TGATATGGTT GATGTACATT TGCTCTTAAA ATCACTCCAC 
ATTGTCGAAG ATGATCTAAA CAAACTCAAA AACGCTTCGA GTCAGGACGA GCTTATGGAT 
AATATGAGGC AATTCGGACG CAATGCAGGA GAACTTATAA AACAGGCAGC CAAACGTCAG 
CAAGAACTTA AGGATCCCCA ATTAAGGGAC GATTTAGCAG CTGCTCGGGC GATGCTTAAA 
AAACATTCAA CTATGCTGTT AACTGCATCA AAAGTATACG TTCGTCATCC GGAACTAGAT 
CTAGCAAAAG TAAATCGCGA TTTCATTCTA AAACAAGTTT GCGATGCTGT AAATACTATT 
AGCGATGTTG CCCAAGGAAA GTCATCCCAA CCGACAGATA TATACAGTGG AGCGGGAGAG 
CTGGCTGCAG CATTAGACGA CTTTGACGAA GGAATTGTTA TGGATCCCAT GACCTACAGC 
GAAAAGCGTT CACGTCAATT GCTCGAAGAG CGTCTGGAAA GTATTATTAG TGCAGCTGCA 
TTGATGGCGG ATGCAGATTG TACTCGAGAC GAGAGACGAG AGAGAATTGT GGCCGAATGC 
AATGCTGTGC GACAGGCTTT ACAGGATTTG TTATCTGAAT ATATGTCAAA CATGAGTCAA 
AAAGATAACA GCCCAGGACT CTCTCGAGCG ATTGATCAAA TGTGTCGAAA GACTCGCGAC 
CTAAGAAGGC AATTGCGAAA GGCTGTTGTG GATCATGTTT CGGACTCTTT TTTGGAGACT 
ACAACTCCTT TGCTAGATTT AATTGAAGCT GCAAAATCGG GTAATGAAAA AAAAGTTCGG 
GAAAAGTCCG AAATATTCAC CAAGCACGCT GAAAAACTAG TTGAAGTAGC AAATTTAGTA 
TGTAGCATGT CAAACAATGA AGATGGTGTT AAAATGGTTC GATATGCTGC TGCTCAAATT 
GAAAGTCTTT GTCCGCAAGT AATAAATGCA GCATCGATAT TGACTGTTAG ACCGAATTCA 
AAAGTAGCTC AAGAAAATAT GACTACTTAT CGACAAGCCT GGGAGGTGCA AGTTCGTATT 
TTAACCGAGG CAGTGGATGA TATTACAACA ATTGACGACT TTTTAGCAGT ATCTGAGAAT 
CACATTCTTG AAGATGTAAA CAAATGTGTA ATGGCTTTAC AAGTTGGTGA TGCCAGGGAT 
TTGCGTGCAA CTGCTGGTGC TATTCAAGGT CGATCATCAC GAGTTTGTAA TGTTGTTGAA 
GCTGAAATGG ATAATTATGA ACCATGTATT TACACCAAAC GAGTCTTAGA AGCAGTCAAA 
GTTCTACGAG ATCAAGTTAT GATGAAATTT GACCAACGTG TAGGAGCAGC CGTTGGAGCC 
CTTTCAAATA ACTCCAATAA GGATGTTGAC GAAAATGACT TCATTGATGC TTCTCGTTTG 
GTTTACGACG GAGTTCGTGA AATTCGAAGA GCTGTTTTAA TGAATCGAAG CTCGGAAGAC 
CTTGATACAG ATACTGAATT TGAGCCAGTT GAAGACTTAA CCTTGGAAAC TCGAAGTCGA 
TCAAGTGCTC ATACCGGCGA TCAAACCGTT GACGAATATC CCGATATAAG TGGCATATGT 
ACAGCTCGAG AAGCAATGCG AAAAATGACG GAAGAAGACA AACAAAAAAT TGCTCAGCAA 
GTGGAGTTAT TCCGTAGGGA AAAACTAACT TTTGATTCAG AAGTTGCTAA ATGGGATGAC 
ACTGGAAATG ACATTATTTT TCTGGCCAAA CATATGTGCA TGATTATGAT GGAAATGACG 
GATTTTACCA GAGGACGAGG ACCTTTAAAA ACTACTATGG ATGTTATTAA CGCAGCTAAA 
AAAATTTCGG AAGCTGGTAC AAAGCTGGAT AAACTAACCA GGGAAATAGC AGAGCAATGC 
CCAGAAAGCA GCACGAAAAA GGACCTTTTA GCGTATTTGC AACGCATTGC CCTGTATTGT 
CATCAAATCC AAATAACTTC AAAAGTAAAA GCAGACGTTC AAAATATAAG CGGCGAACTT 
ATAGTTTCTG GGCTGGACAG CGCTACATCG TTAATTCAAG CTGCTAAAAA TTTAATGAAT 
GCCGTTGTAT TAACGGTAAA GTACTCATAT GTGGCATCAA CAAAATATAC CAGGCAAGGA 
ACAGTGTCTT CTCCAATTGT TGTATGGAAG ATGAAAGCAC CAGAAAAAAA ACCATTGGTC 
AGGCCTGAAA AACCAGAAGA AGTACGGGCC AAAGTTCGCA AGGGATCTCA AAAAAAGGTT 
CAAAACCCTA TACATGCATT ATCTGAATTC CAGAGTCCTG CTGACGCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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alpha-Cat cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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