mael cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mael
Entrez Gene ID	40489
Full Name	maelstrom
Synonyms	CG11254,Dmel\CG11254,MAEL,Mael,mae1
General protein information	Preferred Names maelstrom Names CG11254 gene product from transcript CG11254-RD CG11254-PA CG11254-PB CG11254-PC CG11254-PD mael-PA mael-PB mael-PC mael-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 80A1-80A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079493.4	NP_524217.1	maelstrom, isoform A
NM_168958.3	NP_730739.1	maelstrom, isoform B
NM_001316461.1	NP_001303390.1	maelstrom, isoform D
NM_176388.3	NP_788566.1	maelstrom, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAEL	XP_513969.3
Rattus norvegicus (Norway rat)	Mael	NP_001102327.1
Drosophila melanogaster (fruit fly)	mael	NP_524217.1
Xenopus tropicalis (tropical clawed frog)	mael	NP_001072645.1
Homo sapiens (human)	MAEL	NP_116247.1
Mus musculus (house mouse)	Mael	NP_780505.1
Gallus gallus (chicken)	MAEL	XP_416657.2
Canis lupus familiaris (dog)	MAEL	XP_547484.2
	MAEL	XP_001087413.1
Bos taurus (cattle)	MAEL	NP_001033282.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002022	XP_321033.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Polo-mediated phosphorylation of Maelstrom may be a mechanism that controls oocyte determination by inactivating the pachytene checkpoint via the repression of Sir2 in Drosophila ovaries.
Title: Polo-mediated phosphorylation of Maelstrom regulates oocyte determination during oogenesis in Drosophila.

Study shows that the HMG protein Maelstrom is essential for Piwi-mediated silencing in Drosophila.
Title: Transcriptional silencing of transposons by Piwi and maelstrom and its impact on chromatin state and gene expression.

Mael serves as a platform that nucleates other microtubule-organizing center components to form a functional MTOC in early oocyte development
Title: Maelstrom coordinates microtubule organization during Drosophila oogenesis through interaction with components of the MTOC.

Mael ensures proper differentiation in the germline stem cell lineage by repressing miR-7
Title: Drosophila maelstrom ensures proper germline stem cell lineage differentiation by repressing microRNA-7.

characterized a null allele of maelstrom (mael) and showed that
Title: Maelstrom, a Drosophila spindle-class gene, encodes a protein that colocalizes with Vasa and RDE1/AGO1 homolog, Aubergine, in nuage.

The following mael gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mael cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19290	NM_176388.3 Latest version!	Drosophila melanogaster maelstrom (mael), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19291	NM_001316461.1 Latest version!	Drosophila melanogaster maelstrom (mael), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19291	NM_079493.4 Latest version!	Drosophila melanogaster maelstrom (mael), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19291	NM_168958.3 Latest version!	Drosophila melanogaster maelstrom (mael), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mael ( NM_176388.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788566.1 Drosophila melanogaster maelstrom (mael), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa19290
Clone ID Related Accession (Same CDS sequence)	NM_176388.3
Accession Version	NM_176388.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	maelstrom, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_176388.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCCTA AGAAGCATAG TGGGTTTATG ATGTTCGTAA ACGAGTGGAG AAACCGGAAC 
GCCGAGGGCC GACGCATGAC GCTGGCGCAG GCTGTTTCCC ACTGCGGCAC CATATGGGAG 
CCTTTTCAGA AAATGAACAC CCAGCAGCGT GGTCCGTACA ACTCAGGCGG CAAGGACGCA 
AATGTGGCGC AACGCGCCAA AAGGGAAAGT TCCAACGGCC ACGGCCAGGT GGACAAGGCC 
CAAAGGGAGG CCACCGAGAG CTTGATGGAC ATGAAACGTA CCATCGAGCG CCTCGTGCTA 
AACGCCAAGA TGTCCCATGA TCTGGAGAAT GCCAAGTTTG TCTTCGTTGC CTTCAACTAC 
TTCACTAAAG CCTTGACCAC CGACGTCTAT GTGCCCGCCG AGTTTGCGGC ATGCGAATAT 
TCGTTGAAGG AGGGAATCCG ATCCATCTAC AGCACCATGA TTGACCCCGG TCAGATCATT 
TTTGGGCAAG GTAGCGATGC CCTGCTTCAC TCTTCAACCA CTCACGACCT GCCGCTGCCG 
CCCAACGCGC TGGGGGAGAA GAACATGACC AAGCTGTACC GCAACATTGT TGACTACTTA 
TCCAAATGCC AGGGAAAAGG CAAGACCCTA GTAGTCTTTA CACCCGCCGA AAATATTACC 
ATGGTGAAGT CATGCTTCCG CTATTTGGAG TGCGATGATG ACTTCAGGGA CGGGGGCGAA 
AAAATCCAGG TGTTTGATAT CCAGTACTTG CTATTCATAT TGAAGAAGGA GGTCATGAAT 
GTCGCTGATT TGAATGACGA GAAAATCAAC AAATTCGCGA CGGATGCTTT TTTTAAGAAA 
GACTTTTTCG AGTTTACCGC AGGGATTGCG TGTCAGTACC ACGAGGATAA TGATCGAACC 
AAGTACTGTA CCCAGAGTAT GGTGACTAGA TGGGCGTATA CCTTCACTGA TTTTATGTGC 
GGAGACCTGG CCATAACCGT CCAACCCGGA AAGCACATAC CTGCCCAAAC AAAACCCAAC 
TACCTTATCA TAAGCTCGTA CGCCTCCTCA CTCGACCACG AGTCCTCCTT CGACTCTTTT 
TATTCGCTTC CCGGATCTGG GGTCAAGAAG GAAAGCCAAC CCGAAGCTTG TTCGCTTTCT 
AGCAGTCGAC TTTCCGTCGC ATCAAGCTCC TACAAGCCCA TAGACCACAC TTCCTTTGCC 
GCAAATTTAA ACGAAGTTTC TGAATTCCCC AGCCTCGGCA TGCGTAATTC TAGTAAGCAT 
CACGGTATCG CCGCTAGTGC TCAGCGGGAA TGGAACGCTA GGAACTTACC GACGCACTCG 
CGTTTAATTC GGAAGGTTTC AGACAACGAC TTCTCCGTAA ACGGCGCTGA TGGGAAACTT 
AAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788566.1
CDS	334..1722
Translation	MAPKKHSGFMMFVNEWRNRNAEGRRMTLAQAVSHCGTIWEPFQKMNTQQRGPYNSGGKDANVAQRAKRESSNGHGQVDKAQREATESLMDMKRTIERLVLNAKMSHDLENAKFVFVAFNYFTKALTTDVYVPAEFAACEYSLKEGIRSIYSTMIDPGQIIFGQGSDALLHSSTTHDLPLPPNALGEKNMTKLYRNIVDYLSKCQGKGKTLVVFTPAENITMVKSCFRYLECDDDFRDGGEKIQVFDIQYLLFILKKEVMNVADLNDEKINKFATDAFFKKDFFEFTAGIACQYHEDNDRTKYCTQSMVTRWAYTFTDFMCGDLAITVQPGKHIPAQTKPNYLIISSYASSLDHESSFDSFYSLPGSGVKKESQPEACSLSSSRLSVASSSYKPIDHTSFAANLNEVSEFPSLGMRNSSKHHGIAASAQREWNARNLPTHSRLIRKVSDNDFSVNGADGKLKK

Target ORF information:

RefSeq Version	NM_176388.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster maelstrom (mael), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176388.3

ORF Insert Sequence:

ATGGCTCCTA AGAAGCATAG TGGGTTTATG ATGTTCGTAA ACGAGTGGAG AAACCGGAAC 
GCCGAGGGCC GACGCATGAC GCTGGCGCAG GCTGTTTCCC ACTGCGGCAC CATATGGGAG 
CCTTTTCAGA AAATGAACAC CCAGCAGCGT GGTCCGTACA ACTCAGGCGG CAAGGACGCA 
AATGTGGCGC AACGCGCCAA AAGGGAAAGT TCCAACGGCC ACGGCCAGGT GGACAAGGCC 
CAAAGGGAGG CCACCGAGAG CTTGATGGAC ATGAAACGTA CCATCGAGCG CCTCGTGCTA 
AACGCCAAGA TGTCCCATGA TCTGGAGAAT GCCAAGTTTG TCTTCGTTGC CTTCAACTAC 
TTCACTAAAG CCTTGACCAC CGACGTCTAT GTGCCCGCCG AGTTTGCGGC ATGCGAATAT 
TCGTTGAAGG AGGGAATCCG ATCCATCTAC AGCACCATGA TTGACCCCGG TCAGATCATT 
TTTGGGCAAG GTAGCGATGC CCTGCTTCAC TCTTCAACCA CTCACGACCT GCCGCTGCCG 
CCCAACGCGC TGGGGGAGAA GAACATGACC AAGCTGTACC GCAACATTGT TGACTACTTA 
TCCAAATGCC AGGGAAAAGG CAAGACCCTA GTAGTCTTTA CACCCGCCGA AAATATTACC 
ATGGTGAAGT CATGCTTCCG CTATTTGGAG TGCGATGATG ACTTCAGGGA CGGGGGCGAA 
AAAATCCAGG TGTTTGATAT CCAGTACTTG CTATTCATAT TGAAGAAGGA GGTCATGAAT 
GTCGCTGATT TGAATGACGA GAAAATCAAC AAATTCGCGA CGGATGCTTT TTTTAAGAAA 
GACTTTTTCG AGTTTACCGC AGGGATTGCG TGTCAGTACC ACGAGGATAA TGATCGAACC 
AAGTACTGTA CCCAGAGTAT GGTGACTAGA TGGGCGTATA CCTTCACTGA TTTTATGTGC 
GGAGACCTGG CCATAACCGT CCAACCCGGA AAGCACATAC CTGCCCAAAC AAAACCCAAC 
TACCTTATCA TAAGCTCGTA CGCCTCCTCA CTCGACCACG AGTCCTCCTT CGACTCTTTT 
TATTCGCTTC CCGGATCTGG GGTCAAGAAG GAAAGCCAAC CCGAAGCTTG TTCGCTTTCT 
AGCAGTCGAC TTTCCGTCGC ATCAAGCTCC TACAAGCCCA TAGACCACAC TTCCTTTGCC 
GCAAATTTAA ACGAAGTTTC TGAATTCCCC AGCCTCGGCA TGCGTAATTC TAGTAAGCAT 
CACGGTATCG CCGCTAGTGC TCAGCGGGAA TGGAACGCTA GGAACTTACC GACGCACTCG 
CGTTTAATTC GGAAGGTTTC AGACAACGAC TTCTCCGTAA ACGGCGCTGA TGGGAAACTT 
AAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mael ( NM_079493.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524217.1 Drosophila melanogaster maelstrom (mael), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa19291
Clone ID Related Accession (Same CDS sequence)	NM_079493.4 , NM_168958.3 , NM_001316461.1
Accession Version	NM_079493.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	maelstrom, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079493.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCCTA AGAAGCATAG TGGGTTTATG ATGTTCGTAA ACGAGTGGAG AAACCGGAAC 
GCCGAGGGCC GACGCATGAC GCTGGCGCAG GCTGTTTCCC ACTGCGGCAC CATATGGGAG 
AAAATGAACA CCCAGCAGCG TGGTCCGTAC AACTCAGGCG GCAAGGACGC AAATGTGGCG 
CAACGCGCCA AAAGGGAAAG TTCCAACGGC CACGGCCAGG TGGACAAGGC CCAAAGGGAG 
GCCACCGAGA GCTTGATGGA CATGAAACGT ACCATCGAGC GCCTCGTGCT AAACGCCAAG 
ATGTCCCATG ATCTGGAGAA TGCCAAGTTT GTCTTCGTTG CCTTCAACTA CTTCACTAAA 
GCCTTGACCA CCGACGTCTA TGTGCCCGCC GAGTTTGCGG CATGCGAATA TTCGTTGAAG 
GAGGGAATCC GATCCATCTA CAGCACCATG ATTGACCCCG GTCAGATCAT TTTTGGGCAA 
GGTAGCGATG CCCTGCTTCA CTCTTCAACC ACTCACGACC TGCCGCTGCC GCCCAACGCG 
CTGGGGGAGA AGAACATGAC CAAGCTGTAC CGCAACATTG TTGACTACTT ATCCAAATGC 
CAGGGAAAAG GCAAGACCCT AGTAGTCTTT ACACCCGCCG AAAATATTAC CATGGTGAAG 
TCATGCTTCC GCTATTTGGA GTGCGATGAT GACTTCAGGG ACGGGGGCGA AAAAATCCAG 
GTGTTTGATA TCCAGTACTT GCTATTCATA TTGAAGAAGG AGGTCATGAA TGTCGCTGAT 
TTGAATGACG AGAAAATCAA CAAATTCGCG ACGGATGCTT TTTTTAAGAA AGACTTTTTC 
GAGTTTACCG CAGGGATTGC GTGTCAGTAC CACGAGGATA ATGATCGAAC CAAGTACTGT 
ACCCAGAGTA TGGTGACTAG ATGGGCGTAT ACCTTCACTG ATTTTATGTG CGGAGACCTG 
GCCATAACCG TCCAACCCGG AAAGCACATA CCTGCCCAAA CAAAACCCAA CTACCTTATC 
ATAAGCTCGT ACGCCTCCTC ACTCGACCAC GAGTCCTCCT TCGACTCTTT TTATTCGCTT 
CCCGGATCTG GGGTCAAGAA GGAAAGCCAA CCCGAAGCTT GTTCGCTTTC TAGCAGTCGA 
CTTTCCGTCG CATCAAGCTC CTACAAGCCC ATAGACCACA CTTCCTTTGC CGCAAATTTA 
AACGAAGTTT CTGAATTCCC CAGCCTCGGC ATGCGTAATT CTAGTAAGCA TCACGGTATC 
GCCGCTAGTG CTCAGCGGGA ATGGAACGCT AGGAACTTAC CGACGCACTC GCGTTTAATT 
CGGAAGGTTT CAGACAACGA CTTCTCCGTA AACGGCGCTG ATGGGAAACT TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524217.1
CDS	263..1642
Translation	MAPKKHSGFMMFVNEWRNRNAEGRRMTLAQAVSHCGTIWEKMNTQQRGPYNSGGKDANVAQRAKRESSNGHGQVDKAQREATESLMDMKRTIERLVLNAKMSHDLENAKFVFVAFNYFTKALTTDVYVPAEFAACEYSLKEGIRSIYSTMIDPGQIIFGQGSDALLHSSTTHDLPLPPNALGEKNMTKLYRNIVDYLSKCQGKGKTLVVFTPAENITMVKSCFRYLECDDDFRDGGEKIQVFDIQYLLFILKKEVMNVADLNDEKINKFATDAFFKKDFFEFTAGIACQYHEDNDRTKYCTQSMVTRWAYTFTDFMCGDLAITVQPGKHIPAQTKPNYLIISSYASSLDHESSFDSFYSLPGSGVKKESQPEACSLSSSRLSVASSSYKPIDHTSFAANLNEVSEFPSLGMRNSSKHHGIAASAQREWNARNLPTHSRLIRKVSDNDFSVNGADGKLKK

Target ORF information:

RefSeq Version	NM_079493.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster maelstrom (mael), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079493.4

ORF Insert Sequence:

ATGGCTCCTA AGAAGCATAG TGGGTTTATG ATGTTCGTAA ACGAGTGGAG AAACCGGAAC 
GCCGAGGGCC GACGCATGAC GCTGGCGCAG GCTGTTTCCC ACTGCGGCAC CATATGGGAG 
AAAATGAACA CCCAGCAGCG TGGTCCGTAC AACTCAGGCG GCAAGGACGC AAATGTGGCG 
CAACGCGCCA AAAGGGAAAG TTCCAACGGC CACGGCCAGG TGGACAAGGC CCAAAGGGAG 
GCCACCGAGA GCTTGATGGA CATGAAACGT ACCATCGAGC GCCTCGTGCT AAACGCCAAG 
ATGTCCCATG ATCTGGAGAA TGCCAAGTTT GTCTTCGTTG CCTTCAACTA CTTCACTAAA 
GCCTTGACCA CCGACGTCTA TGTGCCCGCC GAGTTTGCGG CATGCGAATA TTCGTTGAAG 
GAGGGAATCC GATCCATCTA CAGCACCATG ATTGACCCCG GTCAGATCAT TTTTGGGCAA 
GGTAGCGATG CCCTGCTTCA CTCTTCAACC ACTCACGACC TGCCGCTGCC GCCCAACGCG 
CTGGGGGAGA AGAACATGAC CAAGCTGTAC CGCAACATTG TTGACTACTT ATCCAAATGC 
CAGGGAAAAG GCAAGACCCT AGTAGTCTTT ACACCCGCCG AAAATATTAC CATGGTGAAG 
TCATGCTTCC GCTATTTGGA GTGCGATGAT GACTTCAGGG ACGGGGGCGA AAAAATCCAG 
GTGTTTGATA TCCAGTACTT GCTATTCATA TTGAAGAAGG AGGTCATGAA TGTCGCTGAT 
TTGAATGACG AGAAAATCAA CAAATTCGCG ACGGATGCTT TTTTTAAGAA AGACTTTTTC 
GAGTTTACCG CAGGGATTGC GTGTCAGTAC CACGAGGATA ATGATCGAAC CAAGTACTGT 
ACCCAGAGTA TGGTGACTAG ATGGGCGTAT ACCTTCACTG ATTTTATGTG CGGAGACCTG 
GCCATAACCG TCCAACCCGG AAAGCACATA CCTGCCCAAA CAAAACCCAA CTACCTTATC 
ATAAGCTCGT ACGCCTCCTC ACTCGACCAC GAGTCCTCCT TCGACTCTTT TTATTCGCTT 
CCCGGATCTG GGGTCAAGAA GGAAAGCCAA CCCGAAGCTT GTTCGCTTTC TAGCAGTCGA 
CTTTCCGTCG CATCAAGCTC CTACAAGCCC ATAGACCACA CTTCCTTTGC CGCAAATTTA 
AACGAAGTTT CTGAATTCCC CAGCCTCGGC ATGCGTAATT CTAGTAAGCA TCACGGTATC 
GCCGCTAGTG CTCAGCGGGA ATGGAACGCT AGGAACTTAC CGACGCACTC GCGTTTAATT 
CGGAAGGTTT CAGACAACGA CTTCTCCGTA AACGGCGCTG ATGGGAAACT TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mael ( NM_168958.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730739.1 Drosophila melanogaster maelstrom (mael), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19291
Clone ID Related Accession (Same CDS sequence)	NM_079493.4 , NM_168958.3 , NM_001316461.1
Accession Version	NM_168958.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	maelstrom, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168958.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCCTA AGAAGCATAG TGGGTTTATG ATGTTCGTAA ACGAGTGGAG AAACCGGAAC 
GCCGAGGGCC GACGCATGAC GCTGGCGCAG GCTGTTTCCC ACTGCGGCAC CATATGGGAG 
AAAATGAACA CCCAGCAGCG TGGTCCGTAC AACTCAGGCG GCAAGGACGC AAATGTGGCG 
CAACGCGCCA AAAGGGAAAG TTCCAACGGC CACGGCCAGG TGGACAAGGC CCAAAGGGAG 
GCCACCGAGA GCTTGATGGA CATGAAACGT ACCATCGAGC GCCTCGTGCT AAACGCCAAG 
ATGTCCCATG ATCTGGAGAA TGCCAAGTTT GTCTTCGTTG CCTTCAACTA CTTCACTAAA 
GCCTTGACCA CCGACGTCTA TGTGCCCGCC GAGTTTGCGG CATGCGAATA TTCGTTGAAG 
GAGGGAATCC GATCCATCTA CAGCACCATG ATTGACCCCG GTCAGATCAT TTTTGGGCAA 
GGTAGCGATG CCCTGCTTCA CTCTTCAACC ACTCACGACC TGCCGCTGCC GCCCAACGCG 
CTGGGGGAGA AGAACATGAC CAAGCTGTAC CGCAACATTG TTGACTACTT ATCCAAATGC 
CAGGGAAAAG GCAAGACCCT AGTAGTCTTT ACACCCGCCG AAAATATTAC CATGGTGAAG 
TCATGCTTCC GCTATTTGGA GTGCGATGAT GACTTCAGGG ACGGGGGCGA AAAAATCCAG 
GTGTTTGATA TCCAGTACTT GCTATTCATA TTGAAGAAGG AGGTCATGAA TGTCGCTGAT 
TTGAATGACG AGAAAATCAA CAAATTCGCG ACGGATGCTT TTTTTAAGAA AGACTTTTTC 
GAGTTTACCG CAGGGATTGC GTGTCAGTAC CACGAGGATA ATGATCGAAC CAAGTACTGT 
ACCCAGAGTA TGGTGACTAG ATGGGCGTAT ACCTTCACTG ATTTTATGTG CGGAGACCTG 
GCCATAACCG TCCAACCCGG AAAGCACATA CCTGCCCAAA CAAAACCCAA CTACCTTATC 
ATAAGCTCGT ACGCCTCCTC ACTCGACCAC GAGTCCTCCT TCGACTCTTT TTATTCGCTT 
CCCGGATCTG GGGTCAAGAA GGAAAGCCAA CCCGAAGCTT GTTCGCTTTC TAGCAGTCGA 
CTTTCCGTCG CATCAAGCTC CTACAAGCCC ATAGACCACA CTTCCTTTGC CGCAAATTTA 
AACGAAGTTT CTGAATTCCC CAGCCTCGGC ATGCGTAATT CTAGTAAGCA TCACGGTATC 
GCCGCTAGTG CTCAGCGGGA ATGGAACGCT AGGAACTTAC CGACGCACTC GCGTTTAATT 
CGGAAGGTTT CAGACAACGA CTTCTCCGTA AACGGCGCTG ATGGGAAACT TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730739.1
CDS	334..1713
Translation	MAPKKHSGFMMFVNEWRNRNAEGRRMTLAQAVSHCGTIWEKMNTQQRGPYNSGGKDANVAQRAKRESSNGHGQVDKAQREATESLMDMKRTIERLVLNAKMSHDLENAKFVFVAFNYFTKALTTDVYVPAEFAACEYSLKEGIRSIYSTMIDPGQIIFGQGSDALLHSSTTHDLPLPPNALGEKNMTKLYRNIVDYLSKCQGKGKTLVVFTPAENITMVKSCFRYLECDDDFRDGGEKIQVFDIQYLLFILKKEVMNVADLNDEKINKFATDAFFKKDFFEFTAGIACQYHEDNDRTKYCTQSMVTRWAYTFTDFMCGDLAITVQPGKHIPAQTKPNYLIISSYASSLDHESSFDSFYSLPGSGVKKESQPEACSLSSSRLSVASSSYKPIDHTSFAANLNEVSEFPSLGMRNSSKHHGIAASAQREWNARNLPTHSRLIRKVSDNDFSVNGADGKLKK

Target ORF information:

RefSeq Version	NM_168958.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster maelstrom (mael), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168958.3

ORF Insert Sequence:

ATGGCTCCTA AGAAGCATAG TGGGTTTATG ATGTTCGTAA ACGAGTGGAG AAACCGGAAC 
GCCGAGGGCC GACGCATGAC GCTGGCGCAG GCTGTTTCCC ACTGCGGCAC CATATGGGAG 
AAAATGAACA CCCAGCAGCG TGGTCCGTAC AACTCAGGCG GCAAGGACGC AAATGTGGCG 
CAACGCGCCA AAAGGGAAAG TTCCAACGGC CACGGCCAGG TGGACAAGGC CCAAAGGGAG 
GCCACCGAGA GCTTGATGGA CATGAAACGT ACCATCGAGC GCCTCGTGCT AAACGCCAAG 
ATGTCCCATG ATCTGGAGAA TGCCAAGTTT GTCTTCGTTG CCTTCAACTA CTTCACTAAA 
GCCTTGACCA CCGACGTCTA TGTGCCCGCC GAGTTTGCGG CATGCGAATA TTCGTTGAAG 
GAGGGAATCC GATCCATCTA CAGCACCATG ATTGACCCCG GTCAGATCAT TTTTGGGCAA 
GGTAGCGATG CCCTGCTTCA CTCTTCAACC ACTCACGACC TGCCGCTGCC GCCCAACGCG 
CTGGGGGAGA AGAACATGAC CAAGCTGTAC CGCAACATTG TTGACTACTT ATCCAAATGC 
CAGGGAAAAG GCAAGACCCT AGTAGTCTTT ACACCCGCCG AAAATATTAC CATGGTGAAG 
TCATGCTTCC GCTATTTGGA GTGCGATGAT GACTTCAGGG ACGGGGGCGA AAAAATCCAG 
GTGTTTGATA TCCAGTACTT GCTATTCATA TTGAAGAAGG AGGTCATGAA TGTCGCTGAT 
TTGAATGACG AGAAAATCAA CAAATTCGCG ACGGATGCTT TTTTTAAGAA AGACTTTTTC 
GAGTTTACCG CAGGGATTGC GTGTCAGTAC CACGAGGATA ATGATCGAAC CAAGTACTGT 
ACCCAGAGTA TGGTGACTAG ATGGGCGTAT ACCTTCACTG ATTTTATGTG CGGAGACCTG 
GCCATAACCG TCCAACCCGG AAAGCACATA CCTGCCCAAA CAAAACCCAA CTACCTTATC 
ATAAGCTCGT ACGCCTCCTC ACTCGACCAC GAGTCCTCCT TCGACTCTTT TTATTCGCTT 
CCCGGATCTG GGGTCAAGAA GGAAAGCCAA CCCGAAGCTT GTTCGCTTTC TAGCAGTCGA 
CTTTCCGTCG CATCAAGCTC CTACAAGCCC ATAGACCACA CTTCCTTTGC CGCAAATTTA 
AACGAAGTTT CTGAATTCCC CAGCCTCGGC ATGCGTAATT CTAGTAAGCA TCACGGTATC 
GCCGCTAGTG CTCAGCGGGA ATGGAACGCT AGGAACTTAC CGACGCACTC GCGTTTAATT 
CGGAAGGTTT CAGACAACGA CTTCTCCGTA AACGGCGCTG ATGGGAAACT TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mael ( NM_001316461.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303390.1 Drosophila melanogaster maelstrom (mael), transcript variant D, mRNA.

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CloneID	OFa19291
Clone ID Related Accession (Same CDS sequence)	NM_079493.4 , NM_168958.3 , NM_001316461.1
Accession Version	NM_001316461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	maelstrom, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCCTA AGAAGCATAG TGGGTTTATG ATGTTCGTAA ACGAGTGGAG AAACCGGAAC 
GCCGAGGGCC GACGCATGAC GCTGGCGCAG GCTGTTTCCC ACTGCGGCAC CATATGGGAG 
AAAATGAACA CCCAGCAGCG TGGTCCGTAC AACTCAGGCG GCAAGGACGC AAATGTGGCG 
CAACGCGCCA AAAGGGAAAG TTCCAACGGC CACGGCCAGG TGGACAAGGC CCAAAGGGAG 
GCCACCGAGA GCTTGATGGA CATGAAACGT ACCATCGAGC GCCTCGTGCT AAACGCCAAG 
ATGTCCCATG ATCTGGAGAA TGCCAAGTTT GTCTTCGTTG CCTTCAACTA CTTCACTAAA 
GCCTTGACCA CCGACGTCTA TGTGCCCGCC GAGTTTGCGG CATGCGAATA TTCGTTGAAG 
GAGGGAATCC GATCCATCTA CAGCACCATG ATTGACCCCG GTCAGATCAT TTTTGGGCAA 
GGTAGCGATG CCCTGCTTCA CTCTTCAACC ACTCACGACC TGCCGCTGCC GCCCAACGCG 
CTGGGGGAGA AGAACATGAC CAAGCTGTAC CGCAACATTG TTGACTACTT ATCCAAATGC 
CAGGGAAAAG GCAAGACCCT AGTAGTCTTT ACACCCGCCG AAAATATTAC CATGGTGAAG 
TCATGCTTCC GCTATTTGGA GTGCGATGAT GACTTCAGGG ACGGGGGCGA AAAAATCCAG 
GTGTTTGATA TCCAGTACTT GCTATTCATA TTGAAGAAGG AGGTCATGAA TGTCGCTGAT 
TTGAATGACG AGAAAATCAA CAAATTCGCG ACGGATGCTT TTTTTAAGAA AGACTTTTTC 
GAGTTTACCG CAGGGATTGC GTGTCAGTAC CACGAGGATA ATGATCGAAC CAAGTACTGT 
ACCCAGAGTA TGGTGACTAG ATGGGCGTAT ACCTTCACTG ATTTTATGTG CGGAGACCTG 
GCCATAACCG TCCAACCCGG AAAGCACATA CCTGCCCAAA CAAAACCCAA CTACCTTATC 
ATAAGCTCGT ACGCCTCCTC ACTCGACCAC GAGTCCTCCT TCGACTCTTT TTATTCGCTT 
CCCGGATCTG GGGTCAAGAA GGAAAGCCAA CCCGAAGCTT GTTCGCTTTC TAGCAGTCGA 
CTTTCCGTCG CATCAAGCTC CTACAAGCCC ATAGACCACA CTTCCTTTGC CGCAAATTTA 
AACGAAGTTT CTGAATTCCC CAGCCTCGGC ATGCGTAATT CTAGTAAGCA TCACGGTATC 
GCCGCTAGTG CTCAGCGGGA ATGGAACGCT AGGAACTTAC CGACGCACTC GCGTTTAATT 
CGGAAGGTTT CAGACAACGA CTTCTCCGTA AACGGCGCTG ATGGGAAACT TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303390.1
CDS	647..2026
Translation	MAPKKHSGFMMFVNEWRNRNAEGRRMTLAQAVSHCGTIWEKMNTQQRGPYNSGGKDANVAQRAKRESSNGHGQVDKAQREATESLMDMKRTIERLVLNAKMSHDLENAKFVFVAFNYFTKALTTDVYVPAEFAACEYSLKEGIRSIYSTMIDPGQIIFGQGSDALLHSSTTHDLPLPPNALGEKNMTKLYRNIVDYLSKCQGKGKTLVVFTPAENITMVKSCFRYLECDDDFRDGGEKIQVFDIQYLLFILKKEVMNVADLNDEKINKFATDAFFKKDFFEFTAGIACQYHEDNDRTKYCTQSMVTRWAYTFTDFMCGDLAITVQPGKHIPAQTKPNYLIISSYASSLDHESSFDSFYSLPGSGVKKESQPEACSLSSSRLSVASSSYKPIDHTSFAANLNEVSEFPSLGMRNSSKHHGIAASAQREWNARNLPTHSRLIRKVSDNDFSVNGADGKLKK

Target ORF information:

RefSeq Version	NM_001316461.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster maelstrom (mael), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316461.1

ORF Insert Sequence:

ATGGCTCCTA AGAAGCATAG TGGGTTTATG ATGTTCGTAA ACGAGTGGAG AAACCGGAAC 
GCCGAGGGCC GACGCATGAC GCTGGCGCAG GCTGTTTCCC ACTGCGGCAC CATATGGGAG 
AAAATGAACA CCCAGCAGCG TGGTCCGTAC AACTCAGGCG GCAAGGACGC AAATGTGGCG 
CAACGCGCCA AAAGGGAAAG TTCCAACGGC CACGGCCAGG TGGACAAGGC CCAAAGGGAG 
GCCACCGAGA GCTTGATGGA CATGAAACGT ACCATCGAGC GCCTCGTGCT AAACGCCAAG 
ATGTCCCATG ATCTGGAGAA TGCCAAGTTT GTCTTCGTTG CCTTCAACTA CTTCACTAAA 
GCCTTGACCA CCGACGTCTA TGTGCCCGCC GAGTTTGCGG CATGCGAATA TTCGTTGAAG 
GAGGGAATCC GATCCATCTA CAGCACCATG ATTGACCCCG GTCAGATCAT TTTTGGGCAA 
GGTAGCGATG CCCTGCTTCA CTCTTCAACC ACTCACGACC TGCCGCTGCC GCCCAACGCG 
CTGGGGGAGA AGAACATGAC CAAGCTGTAC CGCAACATTG TTGACTACTT ATCCAAATGC 
CAGGGAAAAG GCAAGACCCT AGTAGTCTTT ACACCCGCCG AAAATATTAC CATGGTGAAG 
TCATGCTTCC GCTATTTGGA GTGCGATGAT GACTTCAGGG ACGGGGGCGA AAAAATCCAG 
GTGTTTGATA TCCAGTACTT GCTATTCATA TTGAAGAAGG AGGTCATGAA TGTCGCTGAT 
TTGAATGACG AGAAAATCAA CAAATTCGCG ACGGATGCTT TTTTTAAGAA AGACTTTTTC 
GAGTTTACCG CAGGGATTGC GTGTCAGTAC CACGAGGATA ATGATCGAAC CAAGTACTGT 
ACCCAGAGTA TGGTGACTAG ATGGGCGTAT ACCTTCACTG ATTTTATGTG CGGAGACCTG 
GCCATAACCG TCCAACCCGG AAAGCACATA CCTGCCCAAA CAAAACCCAA CTACCTTATC 
ATAAGCTCGT ACGCCTCCTC ACTCGACCAC GAGTCCTCCT TCGACTCTTT TTATTCGCTT 
CCCGGATCTG GGGTCAAGAA GGAAAGCCAA CCCGAAGCTT GTTCGCTTTC TAGCAGTCGA 
CTTTCCGTCG CATCAAGCTC CTACAAGCCC ATAGACCACA CTTCCTTTGC CGCAAATTTA 
AACGAAGTTT CTGAATTCCC CAGCCTCGGC ATGCGTAATT CTAGTAAGCA TCACGGTATC 
GCCGCTAGTG CTCAGCGGGA ATGGAACGCT AGGAACTTAC CGACGCACTC GCGTTTAATT 
CGGAAGGTTT CAGACAACGA CTTCTCCGTA AACGGCGCTG ATGGGAAACT TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mael cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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