Ddx1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx1
Entrez Gene ID	40457
Full Name	Dead-box-1
Synonyms	CG9054,DDX1,Dbp79E,DmDDX1,DmRH12,Dmel\CG9054,anon-79E
General protein information	Preferred Names Dead-box-1 Names CG9054 gene product from transcript CG9054-RA CG9054-PA Ddx1-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 79D3-79D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_079488.4	NP_524212.2	Dead-box-1

Pathways from BioSystems

Homologies

	DDX1	XP_001090643.1
Homo sapiens (human)	DDX1	NP_004930.1
Drosophila melanogaster (fruit fly)	Ddx1	NP_524212.2
Xenopus tropicalis (tropical clawed frog)	ddx1	NP_001011133.1
Rattus norvegicus (Norway rat)	Ddx1	NP_445866.1
Gallus gallus (chicken)	DDX1	NP_989894.1
Canis lupus familiaris (dog)	DDX1	XP_848865.1
Bos taurus (cattle)	DDX1	NP_001068936.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004765	XP_318051.4
Caenorhabditis elegans (roundworm)	Y55F3BR.1	NP_500004.2
Pan troglodytes (chimpanzee)	DDX1	XP_001162067.2
Mus musculus (house mouse)	Ddx1	NP_598801.1
Danio rerio (zebrafish)	ddx1	NP_001184253.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest a role for Drosophila Ddx1 in stress-induced regulation of splicing.
Title: Loss of the Drosophila melanogaster DEAD box protein Ddx1 leads to reduced size and aberrant gametogenesis.

DEAD-box protein Bel has evolutionarily conserved roles in fertility and development.
Title: Belle is a Drosophila DEAD-box protein required for viability and in the germ line.

The following Ddx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19259	NM_079488.4 Latest version!	Drosophila melanogaster Dead-box-1 (Ddx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx1 ( NM_079488.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524212.2 Drosophila melanogaster Dead-box-1 (Ddx1), mRNA.

Price & Availability↑

CloneID	OFa19259
Clone ID Related Accession (Same CDS sequence)	NM_079488.4
Accession Version	NM_079488.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dead-box-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_079488.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGCAT TCGAAGAGTT TGGCGTGCTT CCGGAGCTGG GAATGGCCAC AGACGAATTG 
GACTGGACCC TGCCCACCGA TGTCCAGGCT GAGGCCATTC CTCTGATCCT GGGTGGCGGC 
GATGTACTGA TGGCCGCCGA AACTGGTTCC GGAAAAACGG GTGCCTTCTG CCTCCCCATT 
CTGCAAATCG TGTGGGAGAC CTTGCGTGAT CTGGAGGAGG GGAAGGCTGG CAAGGGCGGT 
GCAATTGGAG GAGCTGTGAC TCCGTGGACC ATGTCCTTCT TCGACCGAGG AAACGCGCTT 
GCCGTCACTC CTGATGGTCT GCGTTGCCAG TCGCGGGAGT TCAAGGAGTG GCACGGGTGT 
CGGGCCACCA CTGGCGTTCG CGGAAAGGGC AAGTTCTACT TTGAGGCCAC GGTAACGGAC 
GAGGGACTGT GTCGTGTGGG CTGGTCTACA CAGCAGGCAA ACCTCGATTT GGGCACCTGT 
CGCATGGGCT TTGGATTCGG CGGCACCGGC AAGAAGTCCA ACAACCGCCA GTTTGATGAC 
TACGGTGAGG CATTCGGCAA GGCAGATGTC ATCGGCTGCC TGCTCGATTT GAAAAACCAG 
GAAGTGAGCT TCACCAAGAA TGGCCAGAAC CTGGGCGTAG CCTTCCGCCT GCCGGACAAC 
CTTGCCAAGG AGACCTTTTA CCCGGCGGTG GTGCTGAAGA ACGCCGAAAT GCAGTTTAAC 
TTTGGAAAGA CTGACTTCAA GTATGCCCCG GGTAACGGGT TCGTTGGCGC TTGCCAGGCT 
GGACCCGAGC ATAGCAAGGC CAATCCGATA ACCGGTCCCG CAGCTGGAGC ACCGTCCGCC 
AAACCGGCGC CTAACGCCCC TCAGGCCATT ATCATGGAGC CCAGCAGAGA ATTGGCCGAG 
CAAACCTACA ACCAGATCGA GAAGTTTAAA TACCACCTGA GCAACCCAGA GGTTCGCTCC 
CTGCTGCTTA TCGGTGGAGT GAGGTTGGAA GAGCAAAAGG CGCAGCTGAT GCAGGGCACT 
CACATAGTGG TGGGCACGCC CGGGCGGCTG GAGGAGATGA TCAACAGTGG ACTGGTCCTG 
CTCACCCATT GCCGCTTTTT TGTTCTGGAT GAGGCTGACG CACTTCTGAA GCAGGGTTAC 
ACCGAGCTGA TTGACCGTCT ACACAAACAA ATTCCGAAGA TCACATCGGA TGGTCGCCGT 
CTGCAGATGG TCGTCTGCTC GGCCACGTTG CATGCATTTG AGGTGAAGAA AATGGCTGAG 
CGCCTGATGC ACTTCCCCAC CTGGGTGGAT CTGAAGGGGG AGGATGCTGT CCCTGAGACG 
GTTCACCACG TCGTCTGTCT AGTCGACCCG CAGATGGACA CCACCTGGCA GTCGCTGCGC 
CAACCCATTG GCACCGACGG CGTCCACGAC CGTGACAACG TTCATCCAGG CAATCATTCG 
AAGGAGACGT TGTCGCAGGC AGTCAAGCTG CTCAAGGGCG AATACTGTGT GCACGCTATA 
GATAAACACA ATATGGACCG AGCCATTATC TTCTGCCGCA CCAAGCAAGA CTGTGATAAC 
TTAGAAAGAT TTCTCCGCCA GCGAGGCGGA AAACATTACT CGTGCGTCTG CCTTCATGGT 
GACCGAAAGC CGCAGGAGCG TAAAGAAAAC CTGGAAATGT TTAAGCGGCA ACAAGTCAAG 
TTTCTGATTT GCACGGACGT GGCGGCTCGT GGTCTAGACA TTACGGGGCT GCCGTTTATG 
ATAAATGTGA CGCTGCCCGA TGACAAGACC AATTACGTTC ATCGCATCGG ACGTGTGGGC 
AGGGCGGAAC GCATGGGGTT GGCCATTAGT TTGGTGGCAA CAGTGCCGGA GAAGGTTTGG 
TACCACGGCG AGTGGTGCAA GTCGCGCGGC CGTAGCTGCA ACAACACAAA CTTAACCGAA 
GTCCGAGGAT GCTGCATCTG GTACAACGAA CCAAACCTAC TGGCCGAAGT TGAGGACCAC 
TTGAACATCA CCATCCAGCA GGTGGACAAG ACCATGGATG TGCCAGTTAA TGACTTCGAC 
GGCAAGGTCG TGTACGGTCA AAAGAATTTA AGAACTGGCA GCGGCTACGA AGACCACGTC 
GAGCAGCTGG TGCCAACTGT ACGCAAACTG ACGGAGCTAG AGTTGCAATC ACAATCTTTA 
TTCTTGAAAC GTCTTAAGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524212.2
CDS	115..2298
Translation	MTAFEEFGVLPELGMATDELDWTLPTDVQAEAIPLILGGGDVLMAAETGSGKTGAFCLPILQIVWETLRDLEEGKAGKGGAIGGAVTPWTMSFFDRGNALAVTPDGLRCQSREFKEWHGCRATTGVRGKGKFYFEATVTDEGLCRVGWSTQQANLDLGTCRMGFGFGGTGKKSNNRQFDDYGEAFGKADVIGCLLDLKNQEVSFTKNGQNLGVAFRLPDNLAKETFYPAVVLKNAEMQFNFGKTDFKYAPGNGFVGACQAGPEHSKANPITGPAAGAPSAKPAPNAPQAIIMEPSRELAEQTYNQIEKFKYHLSNPEVRSLLLIGGVRLEEQKAQLMQGTHIVVGTPGRLEEMINSGLVLLTHCRFFVLDEADALLKQGYTELIDRLHKQIPKITSDGRRLQMVVCSATLHAFEVKKMAERLMHFPTWVDLKGEDAVPETVHHVVCLVDPQMDTTWQSLRQPIGTDGVHDRDNVHPGNHSKETLSQAVKLLKGEYCVHAIDKHNMDRAIIFCRTKQDCDNLERFLRQRGGKHYSCVCLHGDRKPQERKENLEMFKRQQVKFLICTDVAARGLDITGLPFMINVTLPDDKTNYVHRIGRVGRAERMGLAISLVATVPEKVWYHGEWCKSRGRSCNNTNLTEVRGCCIWYNEPNLLAEVEDHLNITIQQVDKTMDVPVNDFDGKVVYGQKNLRTGSGYEDHVEQLVPTVRKLTELELQSQSLFLKRLKV

Target ORF information:

RefSeq Version	NM_079488.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dead-box-1 (Ddx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079488.4

ORF Insert Sequence:

ATGACTGCAT TCGAAGAGTT TGGCGTGCTT CCGGAGCTGG GAATGGCCAC AGACGAATTG 
GACTGGACCC TGCCCACCGA TGTCCAGGCT GAGGCCATTC CTCTGATCCT GGGTGGCGGC 
GATGTACTGA TGGCCGCCGA AACTGGTTCC GGAAAAACGG GTGCCTTCTG CCTCCCCATT 
CTGCAAATCG TGTGGGAGAC CTTGCGTGAT CTGGAGGAGG GGAAGGCTGG CAAGGGCGGT 
GCAATTGGAG GAGCTGTGAC TCCGTGGACC ATGTCCTTCT TCGACCGAGG AAACGCGCTT 
GCCGTCACTC CTGATGGTCT GCGTTGCCAG TCGCGGGAGT TCAAGGAGTG GCACGGGTGT 
CGGGCCACCA CTGGCGTTCG CGGAAAGGGC AAGTTCTACT TTGAGGCCAC GGTAACGGAC 
GAGGGACTGT GTCGTGTGGG CTGGTCTACA CAGCAGGCAA ACCTCGATTT GGGCACCTGT 
CGCATGGGCT TTGGATTCGG CGGCACCGGC AAGAAGTCCA ACAACCGCCA GTTTGATGAC 
TACGGTGAGG CATTCGGCAA GGCAGATGTC ATCGGCTGCC TGCTCGATTT GAAAAACCAG 
GAAGTGAGCT TCACCAAGAA TGGCCAGAAC CTGGGCGTAG CCTTCCGCCT GCCGGACAAC 
CTTGCCAAGG AGACCTTTTA CCCGGCGGTG GTGCTGAAGA ACGCCGAAAT GCAGTTTAAC 
TTTGGAAAGA CTGACTTCAA GTATGCCCCG GGTAACGGGT TCGTTGGCGC TTGCCAGGCT 
GGACCCGAGC ATAGCAAGGC CAATCCGATA ACCGGTCCCG CAGCTGGAGC ACCGTCCGCC 
AAACCGGCGC CTAACGCCCC TCAGGCCATT ATCATGGAGC CCAGCAGAGA ATTGGCCGAG 
CAAACCTACA ACCAGATCGA GAAGTTTAAA TACCACCTGA GCAACCCAGA GGTTCGCTCC 
CTGCTGCTTA TCGGTGGAGT GAGGTTGGAA GAGCAAAAGG CGCAGCTGAT GCAGGGCACT 
CACATAGTGG TGGGCACGCC CGGGCGGCTG GAGGAGATGA TCAACAGTGG ACTGGTCCTG 
CTCACCCATT GCCGCTTTTT TGTTCTGGAT GAGGCTGACG CACTTCTGAA GCAGGGTTAC 
ACCGAGCTGA TTGACCGTCT ACACAAACAA ATTCCGAAGA TCACATCGGA TGGTCGCCGT 
CTGCAGATGG TCGTCTGCTC GGCCACGTTG CATGCATTTG AGGTGAAGAA AATGGCTGAG 
CGCCTGATGC ACTTCCCCAC CTGGGTGGAT CTGAAGGGGG AGGATGCTGT CCCTGAGACG 
GTTCACCACG TCGTCTGTCT AGTCGACCCG CAGATGGACA CCACCTGGCA GTCGCTGCGC 
CAACCCATTG GCACCGACGG CGTCCACGAC CGTGACAACG TTCATCCAGG CAATCATTCG 
AAGGAGACGT TGTCGCAGGC AGTCAAGCTG CTCAAGGGCG AATACTGTGT GCACGCTATA 
GATAAACACA ATATGGACCG AGCCATTATC TTCTGCCGCA CCAAGCAAGA CTGTGATAAC 
TTAGAAAGAT TTCTCCGCCA GCGAGGCGGA AAACATTACT CGTGCGTCTG CCTTCATGGT 
GACCGAAAGC CGCAGGAGCG TAAAGAAAAC CTGGAAATGT TTAAGCGGCA ACAAGTCAAG 
TTTCTGATTT GCACGGACGT GGCGGCTCGT GGTCTAGACA TTACGGGGCT GCCGTTTATG 
ATAAATGTGA CGCTGCCCGA TGACAAGACC AATTACGTTC ATCGCATCGG ACGTGTGGGC 
AGGGCGGAAC GCATGGGGTT GGCCATTAGT TTGGTGGCAA CAGTGCCGGA GAAGGTTTGG 
TACCACGGCG AGTGGTGCAA GTCGCGCGGC CGTAGCTGCA ACAACACAAA CTTAACCGAA 
GTCCGAGGAT GCTGCATCTG GTACAACGAA CCAAACCTAC TGGCCGAAGT TGAGGACCAC 
TTGAACATCA CCATCCAGCA GGTGGACAAG ACCATGGATG TGCCAGTTAA TGACTTCGAC 
GGCAAGGTCG TGTACGGTCA AAAGAATTTA AGAACTGGCA GCGGCTACGA AGACCACGTC 
GAGCAGCTGG TGCCAACTGT ACGCAAACTG ACGGAGCTAG AGTTGCAATC ACAATCTTTA 
TTCTTGAAAC GTCTTAAGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ddx1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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