P5CDh1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	P5CDh1
Entrez Gene ID	40434
Full Name	delta-1-Pyrroline-5-carboxylate dehydrogenase 1
Synonyms	CG7145,DmP5CDh1,Dmel\CG7145,P5CDh
General protein information	Preferred Names delta-1-Pyrroline-5-carboxylate dehydrogenase 1 Names CG7145 gene product from transcript CG7145-RD CG7145-PA CG7145-PB CG7145-PD CG7145-PE CG7145-PF P5CDh1-PA P5CDh1-PB P5CDh1-PD P5CDh1-PE P5CDh1-PF delta-1-pyrroline-5-carboxylate dehydrogenase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78F4-78F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_168942.5	NP_730695.1	delta-1-Pyrroline-5-carboxylate dehydrogenase 1, isoform A
NM_001202230.2	NP_001189159.1	delta-1-Pyrroline-5-carboxylate dehydrogenase 1, isoform E
NM_141111.6	NP_649368.2	delta-1-Pyrroline-5-carboxylate dehydrogenase 1, isoform B
NM_168945.5	NP_730698.1	delta-1-Pyrroline-5-carboxylate dehydrogenase 1, isoform D
NM_001275267.1	NP_001262196.1	delta-1-Pyrroline-5-carboxylate dehydrogenase 1, isoform F

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ALDH4A1	XP_003307883.1
Mus musculus (house mouse)	Aldh4a1	NP_780647.3
Rattus norvegicus (Norway rat)	Aldh4a1	NP_001128170.1
Gallus gallus (chicken)	ALDH4A1	NP_001239033.1
Magnaporthe oryzae (rice blast fungus)	MGG_17513	XP_003718931.1
Neurospora crassa	NCU03076	XP_964169.1
Homo sapiens (human)	ALDH4A1	NP_003739.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004366	XP_313649.4
	LOC714870	XP_001104847.2
Canis lupus familiaris (dog)	ALDH4A1	XP_855272.2
Caenorhabditis elegans (roundworm)	alh-6	NP_493946.1
Schizosaccharomyces pombe (fission yeast)	SPBC24C6.04	NP_595958.1
Xenopus tropicalis (tropical clawed frog)	aldh4a1	NP_001096184.1
Bos taurus (cattle)	ALDH4A1	NP_001099116.1
Danio rerio (zebrafish)	aldh4a1	NP_957452.1
Drosophila melanogaster (fruit fly)	P5CDh1	NP_730698.1
Saccharomyces cerevisiae (baker's yeast)	PUT2	NP_011902.1
Kluyveromyces lactis	KLLA0C10549g	XP_452670.1
Eremothecium gossypii	AGOS_AAL071C	NP_982471.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Homozygous (CG7145(f04633)/CG7145(f04633)) individuals displayed proline levels twice that of normal, swollen mitochondria, and ultimately larval and pupal lethality.
Title: Drosophila delta-1-pyrroline-5-carboxylate dehydrogenase (P5CDh) is required for proline breakdown and mitochondrial integrity-Establishing a fly model for human type II hyperprolinemia.

The following P5CDh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P5CDh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19239	NM_168942.5 Latest version!	Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa19239	NM_141111.6 Latest version!	Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa19239	NM_168945.5 Latest version!	Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa19239	NM_001202230.2 Latest version!	Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa19239	NM_001275267.1 Latest version!	Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P5CDh1 ( NM_168942.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730695.1 Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa19239
Clone ID Related Accession (Same CDS sequence)	NM_168942.5 , NM_001202230.2 , NM_141111.6 , NM_168945.5 , NM_001275267.1
Accession Version	NM_168942.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	delta-1-Pyrroline-5-carboxylate dehydrogenase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168942.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGAA TGATGCGAAG TTCCTCCTCC AGATGCTCTA GTCTTCAGCA GAGCTTGTTG 
AAAAGTTCGA CACGCTGTCT GGGTTCCGTT ATACCGGACC TTAAACTGAA GGACTTCCCG 
ATCGCCAATG AGCCAATCCT GGGATATCTC AAGGACTCTA AGGAGCGTAA GGCCCTCGAG 
CAGGCTCTAA AGGGCACGGC CTCGAGTTGC GAAGACATCC CCATCGTGAT CGGCGGCAAG 
GAGTACAAGA CGCCGGAGGT TCGATACCAG GTCATGCCCC ATGACCACCA ACACAAGCTG 
GCCAGCTTCT ACTACGCCGA CAAGAAGCTT ATCGAGAAGG CCATCAAAAC GGCGGTGGAA 
ACACAGCCCA AGTGGGACCG TGTGTCGATT GCAGACCGTC TGAAGATCTG GGAGAAGGCA 
GCGGACCTAA TGGCAACGAC GTACCGCCAG GATCTCAACG CCGCCACCAT GCTGGGTCAG 
TCAAAGACGG CGATTCAGGC GGAAATCGAT TCAGCAGCGG AGCTTATCGA CTTTATACGA 
ATGAATGCCT ATTTTCTGAA GGAGGTCACT AAATACCAGC CCATCAGCGA GAACATAAAG 
GTCACGAAGA ACTCCTTGCG CTATCGTGGC ATTGATGGCT TCATCGCCGC TGTCAGCCCC 
TTTAACTTTA CGGCCATCGG CGGCAACTTG TCCTACACGC CAGCTCTGAT GGGAAACGGC 
GTGCTGTGGA AGCCCTCGGA CACTGCCATG CTGTCCAACT GGATTATTTT CAAGATTATG 
CGCGAAGCGG GAGTGCCCGA TGGAGTCGTT AACTTTGTGC CCGCCGATGG GCCCGTGTTT 
GGAGACACGA TTACGGCCAG TCCCCACCTG GCAGGCATCA ATTTCACCGG CTCTGTGCCA 
ACCTTTAACC GCCTGTGGAA GCAGGTGGGC AACAACATTG ACAACTACGT GAACTTTCCT 
CGCTTGACTG GTGAGTGTGG CGGCAAGAAT TTCCACTTTA TACATGCATC AGCCGACGTT 
GAGTCAGTGG TGACCTCCAC GATACGCTCA GCATTTGAGT ACTGCGGCCA GAAGTGTTCC 
GCCTGCTCGC GAATGTATGT GCCGGAATCC CTTTGGCCAC AGATCAAGGA GGGCCTGGTA 
TGCGAGGCAG CAAAGCTGAA GATCGGTGAC GTTCAAGACT TCAGCAGCTT CACGTCGGCT 
GTGATTGATG ACAAAGCATT TAAACGCATT ACGGGCTACA TTGAGCATGC CAAAAAATCC 
CCGAACCTCG AGATTCTCGC CGGAGGCACT TACTCAGACA GCAAGGGCTA CTTCGTTAAT 
CCGACCATTG TACTCAGCAA GGATCCCAAG GACCGGATCA TGACCGAAGA GATTTTCGGT 
CCCGTGCTAT CTATTTATGT GTACAAGGAG TCGGACCTCC TTGAGACCAT GAAACTGGTG 
CACACCTCTA CCAAGTTTGC TTTAACCGGA GCAGTATTCG GGCAGGACGA GGATTTCGTC 
AAGTGCGCTC TGCAGGAGTT TAAAATGGCC GCTGGAAACT TCTACATCAA CGACAAGTCC 
ACTGGATCCG TGGTGGGCCA GCAGCCATTC GGAGGCGGCC GGATGTCCGG CACCAATGAC 
AAGGCCGGAG GACCACATTA TATTCTGCGC TGGACCTCTC CGCAGTCCAT CAAGGAGACC 
TTTGTGCCGC TGCGGGACGT CAACTATCCA TACATGTGCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730695.1
CDS	228..1952
Translation	MLRMMRSSSSRCSSLQQSLLKSSTRCLGSVIPDLKLKDFPIANEPILGYLKDSKERKALEQALKGTASSCEDIPIVIGGKEYKTPEVRYQVMPHDHQHKLASFYYADKKLIEKAIKTAVETQPKWDRVSIADRLKIWEKAADLMATTYRQDLNAATMLGQSKTAIQAEIDSAAELIDFIRMNAYFLKEVTKYQPISENIKVTKNSLRYRGIDGFIAAVSPFNFTAIGGNLSYTPALMGNGVLWKPSDTAMLSNWIIFKIMREAGVPDGVVNFVPADGPVFGDTITASPHLAGINFTGSVPTFNRLWKQVGNNIDNYVNFPRLTGECGGKNFHFIHASADVESVVTSTIRSAFEYCGQKCSACSRMYVPESLWPQIKEGLVCEAAKLKIGDVQDFSSFTSAVIDDKAFKRITGYIEHAKKSPNLEILAGGTYSDSKGYFVNPTIVLSKDPKDRIMTEEIFGPVLSIYVYKESDLLETMKLVHTSTKFALTGAVFGQDEDFVKCALQEFKMAAGNFYINDKSTGSVVGQQPFGGGRMSGTNDKAGGPHYILRWTSPQSIKETFVPLRDVNYPYMCE

Target ORF information:

RefSeq Version	NM_168942.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168942.5

ORF Insert Sequence:

ATGTTGCGAA TGATGCGAAG TTCCTCCTCC AGATGCTCTA GTCTTCAGCA GAGCTTGTTG 
AAAAGTTCGA CACGCTGTCT GGGTTCCGTT ATACCGGACC TTAAACTGAA GGACTTCCCG 
ATCGCCAATG AGCCAATCCT GGGATATCTC AAGGACTCTA AGGAGCGTAA GGCCCTCGAG 
CAGGCTCTAA AGGGCACGGC CTCGAGTTGC GAAGACATCC CCATCGTGAT CGGCGGCAAG 
GAGTACAAGA CGCCGGAGGT TCGATACCAG GTCATGCCCC ATGACCACCA ACACAAGCTG 
GCCAGCTTCT ACTACGCCGA CAAGAAGCTT ATCGAGAAGG CCATCAAAAC GGCGGTGGAA 
ACACAGCCCA AGTGGGACCG TGTGTCGATT GCAGACCGTC TGAAGATCTG GGAGAAGGCA 
GCGGACCTAA TGGCAACGAC GTACCGCCAG GATCTCAACG CCGCCACCAT GCTGGGTCAG 
TCAAAGACGG CGATTCAGGC GGAAATCGAT TCAGCAGCGG AGCTTATCGA CTTTATACGA 
ATGAATGCCT ATTTTCTGAA GGAGGTCACT AAATACCAGC CCATCAGCGA GAACATAAAG 
GTCACGAAGA ACTCCTTGCG CTATCGTGGC ATTGATGGCT TCATCGCCGC TGTCAGCCCC 
TTTAACTTTA CGGCCATCGG CGGCAACTTG TCCTACACGC CAGCTCTGAT GGGAAACGGC 
GTGCTGTGGA AGCCCTCGGA CACTGCCATG CTGTCCAACT GGATTATTTT CAAGATTATG 
CGCGAAGCGG GAGTGCCCGA TGGAGTCGTT AACTTTGTGC CCGCCGATGG GCCCGTGTTT 
GGAGACACGA TTACGGCCAG TCCCCACCTG GCAGGCATCA ATTTCACCGG CTCTGTGCCA 
ACCTTTAACC GCCTGTGGAA GCAGGTGGGC AACAACATTG ACAACTACGT GAACTTTCCT 
CGCTTGACTG GTGAGTGTGG CGGCAAGAAT TTCCACTTTA TACATGCATC AGCCGACGTT 
GAGTCAGTGG TGACCTCCAC GATACGCTCA GCATTTGAGT ACTGCGGCCA GAAGTGTTCC 
GCCTGCTCGC GAATGTATGT GCCGGAATCC CTTTGGCCAC AGATCAAGGA GGGCCTGGTA 
TGCGAGGCAG CAAAGCTGAA GATCGGTGAC GTTCAAGACT TCAGCAGCTT CACGTCGGCT 
GTGATTGATG ACAAAGCATT TAAACGCATT ACGGGCTACA TTGAGCATGC CAAAAAATCC 
CCGAACCTCG AGATTCTCGC CGGAGGCACT TACTCAGACA GCAAGGGCTA CTTCGTTAAT 
CCGACCATTG TACTCAGCAA GGATCCCAAG GACCGGATCA TGACCGAAGA GATTTTCGGT 
CCCGTGCTAT CTATTTATGT GTACAAGGAG TCGGACCTCC TTGAGACCAT GAAACTGGTG 
CACACCTCTA CCAAGTTTGC TTTAACCGGA GCAGTATTCG GGCAGGACGA GGATTTCGTC 
AAGTGCGCTC TGCAGGAGTT TAAAATGGCC GCTGGAAACT TCTACATCAA CGACAAGTCC 
ACTGGATCCG TGGTGGGCCA GCAGCCATTC GGAGGCGGCC GGATGTCCGG CACCAATGAC 
AAGGCCGGAG GACCACATTA TATTCTGCGC TGGACCTCTC CGCAGTCCAT CAAGGAGACC 
TTTGTGCCGC TGCGGGACGT CAACTATCCA TACATGTGCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P5CDh1 ( NM_001202230.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189159.1 Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa19239
Clone ID Related Accession (Same CDS sequence)	NM_168942.5 , NM_001202230.2 , NM_141111.6 , NM_168945.5 , NM_001275267.1
Accession Version	NM_001202230.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	delta-1-Pyrroline-5-carboxylate dehydrogenase 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202230.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGAA TGATGCGAAG TTCCTCCTCC AGATGCTCTA GTCTTCAGCA GAGCTTGTTG 
AAAAGTTCGA CACGCTGTCT GGGTTCCGTT ATACCGGACC TTAAACTGAA GGACTTCCCG 
ATCGCCAATG AGCCAATCCT GGGATATCTC AAGGACTCTA AGGAGCGTAA GGCCCTCGAG 
CAGGCTCTAA AGGGCACGGC CTCGAGTTGC GAAGACATCC CCATCGTGAT CGGCGGCAAG 
GAGTACAAGA CGCCGGAGGT TCGATACCAG GTCATGCCCC ATGACCACCA ACACAAGCTG 
GCCAGCTTCT ACTACGCCGA CAAGAAGCTT ATCGAGAAGG CCATCAAAAC GGCGGTGGAA 
ACACAGCCCA AGTGGGACCG TGTGTCGATT GCAGACCGTC TGAAGATCTG GGAGAAGGCA 
GCGGACCTAA TGGCAACGAC GTACCGCCAG GATCTCAACG CCGCCACCAT GCTGGGTCAG 
TCAAAGACGG CGATTCAGGC GGAAATCGAT TCAGCAGCGG AGCTTATCGA CTTTATACGA 
ATGAATGCCT ATTTTCTGAA GGAGGTCACT AAATACCAGC CCATCAGCGA GAACATAAAG 
GTCACGAAGA ACTCCTTGCG CTATCGTGGC ATTGATGGCT TCATCGCCGC TGTCAGCCCC 
TTTAACTTTA CGGCCATCGG CGGCAACTTG TCCTACACGC CAGCTCTGAT GGGAAACGGC 
GTGCTGTGGA AGCCCTCGGA CACTGCCATG CTGTCCAACT GGATTATTTT CAAGATTATG 
CGCGAAGCGG GAGTGCCCGA TGGAGTCGTT AACTTTGTGC CCGCCGATGG GCCCGTGTTT 
GGAGACACGA TTACGGCCAG TCCCCACCTG GCAGGCATCA ATTTCACCGG CTCTGTGCCA 
ACCTTTAACC GCCTGTGGAA GCAGGTGGGC AACAACATTG ACAACTACGT GAACTTTCCT 
CGCTTGACTG GTGAGTGTGG CGGCAAGAAT TTCCACTTTA TACATGCATC AGCCGACGTT 
GAGTCAGTGG TGACCTCCAC GATACGCTCA GCATTTGAGT ACTGCGGCCA GAAGTGTTCC 
GCCTGCTCGC GAATGTATGT GCCGGAATCC CTTTGGCCAC AGATCAAGGA GGGCCTGGTA 
TGCGAGGCAG CAAAGCTGAA GATCGGTGAC GTTCAAGACT TCAGCAGCTT CACGTCGGCT 
GTGATTGATG ACAAAGCATT TAAACGCATT ACGGGCTACA TTGAGCATGC CAAAAAATCC 
CCGAACCTCG AGATTCTCGC CGGAGGCACT TACTCAGACA GCAAGGGCTA CTTCGTTAAT 
CCGACCATTG TACTCAGCAA GGATCCCAAG GACCGGATCA TGACCGAAGA GATTTTCGGT 
CCCGTGCTAT CTATTTATGT GTACAAGGAG TCGGACCTCC TTGAGACCAT GAAACTGGTG 
CACACCTCTA CCAAGTTTGC TTTAACCGGA GCAGTATTCG GGCAGGACGA GGATTTCGTC 
AAGTGCGCTC TGCAGGAGTT TAAAATGGCC GCTGGAAACT TCTACATCAA CGACAAGTCC 
ACTGGATCCG TGGTGGGCCA GCAGCCATTC GGAGGCGGCC GGATGTCCGG CACCAATGAC 
AAGGCCGGAG GACCACATTA TATTCTGCGC TGGACCTCTC CGCAGTCCAT CAAGGAGACC 
TTTGTGCCGC TGCGGGACGT CAACTATCCA TACATGTGCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189159.1
CDS	131..1855
Translation	MLRMMRSSSSRCSSLQQSLLKSSTRCLGSVIPDLKLKDFPIANEPILGYLKDSKERKALEQALKGTASSCEDIPIVIGGKEYKTPEVRYQVMPHDHQHKLASFYYADKKLIEKAIKTAVETQPKWDRVSIADRLKIWEKAADLMATTYRQDLNAATMLGQSKTAIQAEIDSAAELIDFIRMNAYFLKEVTKYQPISENIKVTKNSLRYRGIDGFIAAVSPFNFTAIGGNLSYTPALMGNGVLWKPSDTAMLSNWIIFKIMREAGVPDGVVNFVPADGPVFGDTITASPHLAGINFTGSVPTFNRLWKQVGNNIDNYVNFPRLTGECGGKNFHFIHASADVESVVTSTIRSAFEYCGQKCSACSRMYVPESLWPQIKEGLVCEAAKLKIGDVQDFSSFTSAVIDDKAFKRITGYIEHAKKSPNLEILAGGTYSDSKGYFVNPTIVLSKDPKDRIMTEEIFGPVLSIYVYKESDLLETMKLVHTSTKFALTGAVFGQDEDFVKCALQEFKMAAGNFYINDKSTGSVVGQQPFGGGRMSGTNDKAGGPHYILRWTSPQSIKETFVPLRDVNYPYMCE

Target ORF information:

RefSeq Version	NM_001202230.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202230.2

ORF Insert Sequence:

ATGTTGCGAA TGATGCGAAG TTCCTCCTCC AGATGCTCTA GTCTTCAGCA GAGCTTGTTG 
AAAAGTTCGA CACGCTGTCT GGGTTCCGTT ATACCGGACC TTAAACTGAA GGACTTCCCG 
ATCGCCAATG AGCCAATCCT GGGATATCTC AAGGACTCTA AGGAGCGTAA GGCCCTCGAG 
CAGGCTCTAA AGGGCACGGC CTCGAGTTGC GAAGACATCC CCATCGTGAT CGGCGGCAAG 
GAGTACAAGA CGCCGGAGGT TCGATACCAG GTCATGCCCC ATGACCACCA ACACAAGCTG 
GCCAGCTTCT ACTACGCCGA CAAGAAGCTT ATCGAGAAGG CCATCAAAAC GGCGGTGGAA 
ACACAGCCCA AGTGGGACCG TGTGTCGATT GCAGACCGTC TGAAGATCTG GGAGAAGGCA 
GCGGACCTAA TGGCAACGAC GTACCGCCAG GATCTCAACG CCGCCACCAT GCTGGGTCAG 
TCAAAGACGG CGATTCAGGC GGAAATCGAT TCAGCAGCGG AGCTTATCGA CTTTATACGA 
ATGAATGCCT ATTTTCTGAA GGAGGTCACT AAATACCAGC CCATCAGCGA GAACATAAAG 
GTCACGAAGA ACTCCTTGCG CTATCGTGGC ATTGATGGCT TCATCGCCGC TGTCAGCCCC 
TTTAACTTTA CGGCCATCGG CGGCAACTTG TCCTACACGC CAGCTCTGAT GGGAAACGGC 
GTGCTGTGGA AGCCCTCGGA CACTGCCATG CTGTCCAACT GGATTATTTT CAAGATTATG 
CGCGAAGCGG GAGTGCCCGA TGGAGTCGTT AACTTTGTGC CCGCCGATGG GCCCGTGTTT 
GGAGACACGA TTACGGCCAG TCCCCACCTG GCAGGCATCA ATTTCACCGG CTCTGTGCCA 
ACCTTTAACC GCCTGTGGAA GCAGGTGGGC AACAACATTG ACAACTACGT GAACTTTCCT 
CGCTTGACTG GTGAGTGTGG CGGCAAGAAT TTCCACTTTA TACATGCATC AGCCGACGTT 
GAGTCAGTGG TGACCTCCAC GATACGCTCA GCATTTGAGT ACTGCGGCCA GAAGTGTTCC 
GCCTGCTCGC GAATGTATGT GCCGGAATCC CTTTGGCCAC AGATCAAGGA GGGCCTGGTA 
TGCGAGGCAG CAAAGCTGAA GATCGGTGAC GTTCAAGACT TCAGCAGCTT CACGTCGGCT 
GTGATTGATG ACAAAGCATT TAAACGCATT ACGGGCTACA TTGAGCATGC CAAAAAATCC 
CCGAACCTCG AGATTCTCGC CGGAGGCACT TACTCAGACA GCAAGGGCTA CTTCGTTAAT 
CCGACCATTG TACTCAGCAA GGATCCCAAG GACCGGATCA TGACCGAAGA GATTTTCGGT 
CCCGTGCTAT CTATTTATGT GTACAAGGAG TCGGACCTCC TTGAGACCAT GAAACTGGTG 
CACACCTCTA CCAAGTTTGC TTTAACCGGA GCAGTATTCG GGCAGGACGA GGATTTCGTC 
AAGTGCGCTC TGCAGGAGTT TAAAATGGCC GCTGGAAACT TCTACATCAA CGACAAGTCC 
ACTGGATCCG TGGTGGGCCA GCAGCCATTC GGAGGCGGCC GGATGTCCGG CACCAATGAC 
AAGGCCGGAG GACCACATTA TATTCTGCGC TGGACCTCTC CGCAGTCCAT CAAGGAGACC 
TTTGTGCCGC TGCGGGACGT CAACTATCCA TACATGTGCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P5CDh1 ( NM_141111.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649368.2 Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19239
Clone ID Related Accession (Same CDS sequence)	NM_168942.5 , NM_001202230.2 , NM_141111.6 , NM_168945.5 , NM_001275267.1
Accession Version	NM_141111.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	delta-1-Pyrroline-5-carboxylate dehydrogenase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_141111.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGAA TGATGCGAAG TTCCTCCTCC AGATGCTCTA GTCTTCAGCA GAGCTTGTTG 
AAAAGTTCGA CACGCTGTCT GGGTTCCGTT ATACCGGACC TTAAACTGAA GGACTTCCCG 
ATCGCCAATG AGCCAATCCT GGGATATCTC AAGGACTCTA AGGAGCGTAA GGCCCTCGAG 
CAGGCTCTAA AGGGCACGGC CTCGAGTTGC GAAGACATCC CCATCGTGAT CGGCGGCAAG 
GAGTACAAGA CGCCGGAGGT TCGATACCAG GTCATGCCCC ATGACCACCA ACACAAGCTG 
GCCAGCTTCT ACTACGCCGA CAAGAAGCTT ATCGAGAAGG CCATCAAAAC GGCGGTGGAA 
ACACAGCCCA AGTGGGACCG TGTGTCGATT GCAGACCGTC TGAAGATCTG GGAGAAGGCA 
GCGGACCTAA TGGCAACGAC GTACCGCCAG GATCTCAACG CCGCCACCAT GCTGGGTCAG 
TCAAAGACGG CGATTCAGGC GGAAATCGAT TCAGCAGCGG AGCTTATCGA CTTTATACGA 
ATGAATGCCT ATTTTCTGAA GGAGGTCACT AAATACCAGC CCATCAGCGA GAACATAAAG 
GTCACGAAGA ACTCCTTGCG CTATCGTGGC ATTGATGGCT TCATCGCCGC TGTCAGCCCC 
TTTAACTTTA CGGCCATCGG CGGCAACTTG TCCTACACGC CAGCTCTGAT GGGAAACGGC 
GTGCTGTGGA AGCCCTCGGA CACTGCCATG CTGTCCAACT GGATTATTTT CAAGATTATG 
CGCGAAGCGG GAGTGCCCGA TGGAGTCGTT AACTTTGTGC CCGCCGATGG GCCCGTGTTT 
GGAGACACGA TTACGGCCAG TCCCCACCTG GCAGGCATCA ATTTCACCGG CTCTGTGCCA 
ACCTTTAACC GCCTGTGGAA GCAGGTGGGC AACAACATTG ACAACTACGT GAACTTTCCT 
CGCTTGACTG GTGAGTGTGG CGGCAAGAAT TTCCACTTTA TACATGCATC AGCCGACGTT 
GAGTCAGTGG TGACCTCCAC GATACGCTCA GCATTTGAGT ACTGCGGCCA GAAGTGTTCC 
GCCTGCTCGC GAATGTATGT GCCGGAATCC CTTTGGCCAC AGATCAAGGA GGGCCTGGTA 
TGCGAGGCAG CAAAGCTGAA GATCGGTGAC GTTCAAGACT TCAGCAGCTT CACGTCGGCT 
GTGATTGATG ACAAAGCATT TAAACGCATT ACGGGCTACA TTGAGCATGC CAAAAAATCC 
CCGAACCTCG AGATTCTCGC CGGAGGCACT TACTCAGACA GCAAGGGCTA CTTCGTTAAT 
CCGACCATTG TACTCAGCAA GGATCCCAAG GACCGGATCA TGACCGAAGA GATTTTCGGT 
CCCGTGCTAT CTATTTATGT GTACAAGGAG TCGGACCTCC TTGAGACCAT GAAACTGGTG 
CACACCTCTA CCAAGTTTGC TTTAACCGGA GCAGTATTCG GGCAGGACGA GGATTTCGTC 
AAGTGCGCTC TGCAGGAGTT TAAAATGGCC GCTGGAAACT TCTACATCAA CGACAAGTCC 
ACTGGATCCG TGGTGGGCCA GCAGCCATTC GGAGGCGGCC GGATGTCCGG CACCAATGAC 
AAGGCCGGAG GACCACATTA TATTCTGCGC TGGACCTCTC CGCAGTCCAT CAAGGAGACC 
TTTGTGCCGC TGCGGGACGT CAACTATCCA TACATGTGCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649368.2
CDS	179..1903
Translation	MLRMMRSSSSRCSSLQQSLLKSSTRCLGSVIPDLKLKDFPIANEPILGYLKDSKERKALEQALKGTASSCEDIPIVIGGKEYKTPEVRYQVMPHDHQHKLASFYYADKKLIEKAIKTAVETQPKWDRVSIADRLKIWEKAADLMATTYRQDLNAATMLGQSKTAIQAEIDSAAELIDFIRMNAYFLKEVTKYQPISENIKVTKNSLRYRGIDGFIAAVSPFNFTAIGGNLSYTPALMGNGVLWKPSDTAMLSNWIIFKIMREAGVPDGVVNFVPADGPVFGDTITASPHLAGINFTGSVPTFNRLWKQVGNNIDNYVNFPRLTGECGGKNFHFIHASADVESVVTSTIRSAFEYCGQKCSACSRMYVPESLWPQIKEGLVCEAAKLKIGDVQDFSSFTSAVIDDKAFKRITGYIEHAKKSPNLEILAGGTYSDSKGYFVNPTIVLSKDPKDRIMTEEIFGPVLSIYVYKESDLLETMKLVHTSTKFALTGAVFGQDEDFVKCALQEFKMAAGNFYINDKSTGSVVGQQPFGGGRMSGTNDKAGGPHYILRWTSPQSIKETFVPLRDVNYPYMCE

Target ORF information:

RefSeq Version	NM_141111.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141111.6

ORF Insert Sequence:

ATGTTGCGAA TGATGCGAAG TTCCTCCTCC AGATGCTCTA GTCTTCAGCA GAGCTTGTTG 
AAAAGTTCGA CACGCTGTCT GGGTTCCGTT ATACCGGACC TTAAACTGAA GGACTTCCCG 
ATCGCCAATG AGCCAATCCT GGGATATCTC AAGGACTCTA AGGAGCGTAA GGCCCTCGAG 
CAGGCTCTAA AGGGCACGGC CTCGAGTTGC GAAGACATCC CCATCGTGAT CGGCGGCAAG 
GAGTACAAGA CGCCGGAGGT TCGATACCAG GTCATGCCCC ATGACCACCA ACACAAGCTG 
GCCAGCTTCT ACTACGCCGA CAAGAAGCTT ATCGAGAAGG CCATCAAAAC GGCGGTGGAA 
ACACAGCCCA AGTGGGACCG TGTGTCGATT GCAGACCGTC TGAAGATCTG GGAGAAGGCA 
GCGGACCTAA TGGCAACGAC GTACCGCCAG GATCTCAACG CCGCCACCAT GCTGGGTCAG 
TCAAAGACGG CGATTCAGGC GGAAATCGAT TCAGCAGCGG AGCTTATCGA CTTTATACGA 
ATGAATGCCT ATTTTCTGAA GGAGGTCACT AAATACCAGC CCATCAGCGA GAACATAAAG 
GTCACGAAGA ACTCCTTGCG CTATCGTGGC ATTGATGGCT TCATCGCCGC TGTCAGCCCC 
TTTAACTTTA CGGCCATCGG CGGCAACTTG TCCTACACGC CAGCTCTGAT GGGAAACGGC 
GTGCTGTGGA AGCCCTCGGA CACTGCCATG CTGTCCAACT GGATTATTTT CAAGATTATG 
CGCGAAGCGG GAGTGCCCGA TGGAGTCGTT AACTTTGTGC CCGCCGATGG GCCCGTGTTT 
GGAGACACGA TTACGGCCAG TCCCCACCTG GCAGGCATCA ATTTCACCGG CTCTGTGCCA 
ACCTTTAACC GCCTGTGGAA GCAGGTGGGC AACAACATTG ACAACTACGT GAACTTTCCT 
CGCTTGACTG GTGAGTGTGG CGGCAAGAAT TTCCACTTTA TACATGCATC AGCCGACGTT 
GAGTCAGTGG TGACCTCCAC GATACGCTCA GCATTTGAGT ACTGCGGCCA GAAGTGTTCC 
GCCTGCTCGC GAATGTATGT GCCGGAATCC CTTTGGCCAC AGATCAAGGA GGGCCTGGTA 
TGCGAGGCAG CAAAGCTGAA GATCGGTGAC GTTCAAGACT TCAGCAGCTT CACGTCGGCT 
GTGATTGATG ACAAAGCATT TAAACGCATT ACGGGCTACA TTGAGCATGC CAAAAAATCC 
CCGAACCTCG AGATTCTCGC CGGAGGCACT TACTCAGACA GCAAGGGCTA CTTCGTTAAT 
CCGACCATTG TACTCAGCAA GGATCCCAAG GACCGGATCA TGACCGAAGA GATTTTCGGT 
CCCGTGCTAT CTATTTATGT GTACAAGGAG TCGGACCTCC TTGAGACCAT GAAACTGGTG 
CACACCTCTA CCAAGTTTGC TTTAACCGGA GCAGTATTCG GGCAGGACGA GGATTTCGTC 
AAGTGCGCTC TGCAGGAGTT TAAAATGGCC GCTGGAAACT TCTACATCAA CGACAAGTCC 
ACTGGATCCG TGGTGGGCCA GCAGCCATTC GGAGGCGGCC GGATGTCCGG CACCAATGAC 
AAGGCCGGAG GACCACATTA TATTCTGCGC TGGACCTCTC CGCAGTCCAT CAAGGAGACC 
TTTGTGCCGC TGCGGGACGT CAACTATCCA TACATGTGCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P5CDh1 ( NM_168945.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730698.1 Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant D, mRNA.

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CloneID	OFa19239
Clone ID Related Accession (Same CDS sequence)	NM_168942.5 , NM_001202230.2 , NM_141111.6 , NM_168945.5 , NM_001275267.1
Accession Version	NM_168945.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	delta-1-Pyrroline-5-carboxylate dehydrogenase 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168945.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGAA TGATGCGAAG TTCCTCCTCC AGATGCTCTA GTCTTCAGCA GAGCTTGTTG 
AAAAGTTCGA CACGCTGTCT GGGTTCCGTT ATACCGGACC TTAAACTGAA GGACTTCCCG 
ATCGCCAATG AGCCAATCCT GGGATATCTC AAGGACTCTA AGGAGCGTAA GGCCCTCGAG 
CAGGCTCTAA AGGGCACGGC CTCGAGTTGC GAAGACATCC CCATCGTGAT CGGCGGCAAG 
GAGTACAAGA CGCCGGAGGT TCGATACCAG GTCATGCCCC ATGACCACCA ACACAAGCTG 
GCCAGCTTCT ACTACGCCGA CAAGAAGCTT ATCGAGAAGG CCATCAAAAC GGCGGTGGAA 
ACACAGCCCA AGTGGGACCG TGTGTCGATT GCAGACCGTC TGAAGATCTG GGAGAAGGCA 
GCGGACCTAA TGGCAACGAC GTACCGCCAG GATCTCAACG CCGCCACCAT GCTGGGTCAG 
TCAAAGACGG CGATTCAGGC GGAAATCGAT TCAGCAGCGG AGCTTATCGA CTTTATACGA 
ATGAATGCCT ATTTTCTGAA GGAGGTCACT AAATACCAGC CCATCAGCGA GAACATAAAG 
GTCACGAAGA ACTCCTTGCG CTATCGTGGC ATTGATGGCT TCATCGCCGC TGTCAGCCCC 
TTTAACTTTA CGGCCATCGG CGGCAACTTG TCCTACACGC CAGCTCTGAT GGGAAACGGC 
GTGCTGTGGA AGCCCTCGGA CACTGCCATG CTGTCCAACT GGATTATTTT CAAGATTATG 
CGCGAAGCGG GAGTGCCCGA TGGAGTCGTT AACTTTGTGC CCGCCGATGG GCCCGTGTTT 
GGAGACACGA TTACGGCCAG TCCCCACCTG GCAGGCATCA ATTTCACCGG CTCTGTGCCA 
ACCTTTAACC GCCTGTGGAA GCAGGTGGGC AACAACATTG ACAACTACGT GAACTTTCCT 
CGCTTGACTG GTGAGTGTGG CGGCAAGAAT TTCCACTTTA TACATGCATC AGCCGACGTT 
GAGTCAGTGG TGACCTCCAC GATACGCTCA GCATTTGAGT ACTGCGGCCA GAAGTGTTCC 
GCCTGCTCGC GAATGTATGT GCCGGAATCC CTTTGGCCAC AGATCAAGGA GGGCCTGGTA 
TGCGAGGCAG CAAAGCTGAA GATCGGTGAC GTTCAAGACT TCAGCAGCTT CACGTCGGCT 
GTGATTGATG ACAAAGCATT TAAACGCATT ACGGGCTACA TTGAGCATGC CAAAAAATCC 
CCGAACCTCG AGATTCTCGC CGGAGGCACT TACTCAGACA GCAAGGGCTA CTTCGTTAAT 
CCGACCATTG TACTCAGCAA GGATCCCAAG GACCGGATCA TGACCGAAGA GATTTTCGGT 
CCCGTGCTAT CTATTTATGT GTACAAGGAG TCGGACCTCC TTGAGACCAT GAAACTGGTG 
CACACCTCTA CCAAGTTTGC TTTAACCGGA GCAGTATTCG GGCAGGACGA GGATTTCGTC 
AAGTGCGCTC TGCAGGAGTT TAAAATGGCC GCTGGAAACT TCTACATCAA CGACAAGTCC 
ACTGGATCCG TGGTGGGCCA GCAGCCATTC GGAGGCGGCC GGATGTCCGG CACCAATGAC 
AAGGCCGGAG GACCACATTA TATTCTGCGC TGGACCTCTC CGCAGTCCAT CAAGGAGACC 
TTTGTGCCGC TGCGGGACGT CAACTATCCA TACATGTGCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730698.1
CDS	224..1948
Translation	MLRMMRSSSSRCSSLQQSLLKSSTRCLGSVIPDLKLKDFPIANEPILGYLKDSKERKALEQALKGTASSCEDIPIVIGGKEYKTPEVRYQVMPHDHQHKLASFYYADKKLIEKAIKTAVETQPKWDRVSIADRLKIWEKAADLMATTYRQDLNAATMLGQSKTAIQAEIDSAAELIDFIRMNAYFLKEVTKYQPISENIKVTKNSLRYRGIDGFIAAVSPFNFTAIGGNLSYTPALMGNGVLWKPSDTAMLSNWIIFKIMREAGVPDGVVNFVPADGPVFGDTITASPHLAGINFTGSVPTFNRLWKQVGNNIDNYVNFPRLTGECGGKNFHFIHASADVESVVTSTIRSAFEYCGQKCSACSRMYVPESLWPQIKEGLVCEAAKLKIGDVQDFSSFTSAVIDDKAFKRITGYIEHAKKSPNLEILAGGTYSDSKGYFVNPTIVLSKDPKDRIMTEEIFGPVLSIYVYKESDLLETMKLVHTSTKFALTGAVFGQDEDFVKCALQEFKMAAGNFYINDKSTGSVVGQQPFGGGRMSGTNDKAGGPHYILRWTSPQSIKETFVPLRDVNYPYMCE

Target ORF information:

RefSeq Version	NM_168945.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168945.5

ORF Insert Sequence:

ATGTTGCGAA TGATGCGAAG TTCCTCCTCC AGATGCTCTA GTCTTCAGCA GAGCTTGTTG 
AAAAGTTCGA CACGCTGTCT GGGTTCCGTT ATACCGGACC TTAAACTGAA GGACTTCCCG 
ATCGCCAATG AGCCAATCCT GGGATATCTC AAGGACTCTA AGGAGCGTAA GGCCCTCGAG 
CAGGCTCTAA AGGGCACGGC CTCGAGTTGC GAAGACATCC CCATCGTGAT CGGCGGCAAG 
GAGTACAAGA CGCCGGAGGT TCGATACCAG GTCATGCCCC ATGACCACCA ACACAAGCTG 
GCCAGCTTCT ACTACGCCGA CAAGAAGCTT ATCGAGAAGG CCATCAAAAC GGCGGTGGAA 
ACACAGCCCA AGTGGGACCG TGTGTCGATT GCAGACCGTC TGAAGATCTG GGAGAAGGCA 
GCGGACCTAA TGGCAACGAC GTACCGCCAG GATCTCAACG CCGCCACCAT GCTGGGTCAG 
TCAAAGACGG CGATTCAGGC GGAAATCGAT TCAGCAGCGG AGCTTATCGA CTTTATACGA 
ATGAATGCCT ATTTTCTGAA GGAGGTCACT AAATACCAGC CCATCAGCGA GAACATAAAG 
GTCACGAAGA ACTCCTTGCG CTATCGTGGC ATTGATGGCT TCATCGCCGC TGTCAGCCCC 
TTTAACTTTA CGGCCATCGG CGGCAACTTG TCCTACACGC CAGCTCTGAT GGGAAACGGC 
GTGCTGTGGA AGCCCTCGGA CACTGCCATG CTGTCCAACT GGATTATTTT CAAGATTATG 
CGCGAAGCGG GAGTGCCCGA TGGAGTCGTT AACTTTGTGC CCGCCGATGG GCCCGTGTTT 
GGAGACACGA TTACGGCCAG TCCCCACCTG GCAGGCATCA ATTTCACCGG CTCTGTGCCA 
ACCTTTAACC GCCTGTGGAA GCAGGTGGGC AACAACATTG ACAACTACGT GAACTTTCCT 
CGCTTGACTG GTGAGTGTGG CGGCAAGAAT TTCCACTTTA TACATGCATC AGCCGACGTT 
GAGTCAGTGG TGACCTCCAC GATACGCTCA GCATTTGAGT ACTGCGGCCA GAAGTGTTCC 
GCCTGCTCGC GAATGTATGT GCCGGAATCC CTTTGGCCAC AGATCAAGGA GGGCCTGGTA 
TGCGAGGCAG CAAAGCTGAA GATCGGTGAC GTTCAAGACT TCAGCAGCTT CACGTCGGCT 
GTGATTGATG ACAAAGCATT TAAACGCATT ACGGGCTACA TTGAGCATGC CAAAAAATCC 
CCGAACCTCG AGATTCTCGC CGGAGGCACT TACTCAGACA GCAAGGGCTA CTTCGTTAAT 
CCGACCATTG TACTCAGCAA GGATCCCAAG GACCGGATCA TGACCGAAGA GATTTTCGGT 
CCCGTGCTAT CTATTTATGT GTACAAGGAG TCGGACCTCC TTGAGACCAT GAAACTGGTG 
CACACCTCTA CCAAGTTTGC TTTAACCGGA GCAGTATTCG GGCAGGACGA GGATTTCGTC 
AAGTGCGCTC TGCAGGAGTT TAAAATGGCC GCTGGAAACT TCTACATCAA CGACAAGTCC 
ACTGGATCCG TGGTGGGCCA GCAGCCATTC GGAGGCGGCC GGATGTCCGG CACCAATGAC 
AAGGCCGGAG GACCACATTA TATTCTGCGC TGGACCTCTC CGCAGTCCAT CAAGGAGACC 
TTTGTGCCGC TGCGGGACGT CAACTATCCA TACATGTGCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P5CDh1 ( NM_001275267.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262196.1 Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant F, mRNA.

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CloneID	OFa19239
Clone ID Related Accession (Same CDS sequence)	NM_168942.5 , NM_001202230.2 , NM_141111.6 , NM_168945.5 , NM_001275267.1
Accession Version	NM_001275267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	delta-1-Pyrroline-5-carboxylate dehydrogenase 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGAA TGATGCGAAG TTCCTCCTCC AGATGCTCTA GTCTTCAGCA GAGCTTGTTG 
AAAAGTTCGA CACGCTGTCT GGGTTCCGTT ATACCGGACC TTAAACTGAA GGACTTCCCG 
ATCGCCAATG AGCCAATCCT GGGATATCTC AAGGACTCTA AGGAGCGTAA GGCCCTCGAG 
CAGGCTCTAA AGGGCACGGC CTCGAGTTGC GAAGACATCC CCATCGTGAT CGGCGGCAAG 
GAGTACAAGA CGCCGGAGGT TCGATACCAG GTCATGCCCC ATGACCACCA ACACAAGCTG 
GCCAGCTTCT ACTACGCCGA CAAGAAGCTT ATCGAGAAGG CCATCAAAAC GGCGGTGGAA 
ACACAGCCCA AGTGGGACCG TGTGTCGATT GCAGACCGTC TGAAGATCTG GGAGAAGGCA 
GCGGACCTAA TGGCAACGAC GTACCGCCAG GATCTCAACG CCGCCACCAT GCTGGGTCAG 
TCAAAGACGG CGATTCAGGC GGAAATCGAT TCAGCAGCGG AGCTTATCGA CTTTATACGA 
ATGAATGCCT ATTTTCTGAA GGAGGTCACT AAATACCAGC CCATCAGCGA GAACATAAAG 
GTCACGAAGA ACTCCTTGCG CTATCGTGGC ATTGATGGCT TCATCGCCGC TGTCAGCCCC 
TTTAACTTTA CGGCCATCGG CGGCAACTTG TCCTACACGC CAGCTCTGAT GGGAAACGGC 
GTGCTGTGGA AGCCCTCGGA CACTGCCATG CTGTCCAACT GGATTATTTT CAAGATTATG 
CGCGAAGCGG GAGTGCCCGA TGGAGTCGTT AACTTTGTGC CCGCCGATGG GCCCGTGTTT 
GGAGACACGA TTACGGCCAG TCCCCACCTG GCAGGCATCA ATTTCACCGG CTCTGTGCCA 
ACCTTTAACC GCCTGTGGAA GCAGGTGGGC AACAACATTG ACAACTACGT GAACTTTCCT 
CGCTTGACTG GTGAGTGTGG CGGCAAGAAT TTCCACTTTA TACATGCATC AGCCGACGTT 
GAGTCAGTGG TGACCTCCAC GATACGCTCA GCATTTGAGT ACTGCGGCCA GAAGTGTTCC 
GCCTGCTCGC GAATGTATGT GCCGGAATCC CTTTGGCCAC AGATCAAGGA GGGCCTGGTA 
TGCGAGGCAG CAAAGCTGAA GATCGGTGAC GTTCAAGACT TCAGCAGCTT CACGTCGGCT 
GTGATTGATG ACAAAGCATT TAAACGCATT ACGGGCTACA TTGAGCATGC CAAAAAATCC 
CCGAACCTCG AGATTCTCGC CGGAGGCACT TACTCAGACA GCAAGGGCTA CTTCGTTAAT 
CCGACCATTG TACTCAGCAA GGATCCCAAG GACCGGATCA TGACCGAAGA GATTTTCGGT 
CCCGTGCTAT CTATTTATGT GTACAAGGAG TCGGACCTCC TTGAGACCAT GAAACTGGTG 
CACACCTCTA CCAAGTTTGC TTTAACCGGA GCAGTATTCG GGCAGGACGA GGATTTCGTC 
AAGTGCGCTC TGCAGGAGTT TAAAATGGCC GCTGGAAACT TCTACATCAA CGACAAGTCC 
ACTGGATCCG TGGTGGGCCA GCAGCCATTC GGAGGCGGCC GGATGTCCGG CACCAATGAC 
AAGGCCGGAG GACCACATTA TATTCTGCGC TGGACCTCTC CGCAGTCCAT CAAGGAGACC 
TTTGTGCCGC TGCGGGACGT CAACTATCCA TACATGTGCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262196.1
CDS	275..1999
Translation	MLRMMRSSSSRCSSLQQSLLKSSTRCLGSVIPDLKLKDFPIANEPILGYLKDSKERKALEQALKGTASSCEDIPIVIGGKEYKTPEVRYQVMPHDHQHKLASFYYADKKLIEKAIKTAVETQPKWDRVSIADRLKIWEKAADLMATTYRQDLNAATMLGQSKTAIQAEIDSAAELIDFIRMNAYFLKEVTKYQPISENIKVTKNSLRYRGIDGFIAAVSPFNFTAIGGNLSYTPALMGNGVLWKPSDTAMLSNWIIFKIMREAGVPDGVVNFVPADGPVFGDTITASPHLAGINFTGSVPTFNRLWKQVGNNIDNYVNFPRLTGECGGKNFHFIHASADVESVVTSTIRSAFEYCGQKCSACSRMYVPESLWPQIKEGLVCEAAKLKIGDVQDFSSFTSAVIDDKAFKRITGYIEHAKKSPNLEILAGGTYSDSKGYFVNPTIVLSKDPKDRIMTEEIFGPVLSIYVYKESDLLETMKLVHTSTKFALTGAVFGQDEDFVKCALQEFKMAAGNFYINDKSTGSVVGQQPFGGGRMSGTNDKAGGPHYILRWTSPQSIKETFVPLRDVNYPYMCE

Target ORF information:

RefSeq Version	NM_001275267.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster delta-1-Pyrroline-5-carboxylate dehydrogenase 1 (P5CDh1), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275267.1

ORF Insert Sequence:

ATGTTGCGAA TGATGCGAAG TTCCTCCTCC AGATGCTCTA GTCTTCAGCA GAGCTTGTTG 
AAAAGTTCGA CACGCTGTCT GGGTTCCGTT ATACCGGACC TTAAACTGAA GGACTTCCCG 
ATCGCCAATG AGCCAATCCT GGGATATCTC AAGGACTCTA AGGAGCGTAA GGCCCTCGAG 
CAGGCTCTAA AGGGCACGGC CTCGAGTTGC GAAGACATCC CCATCGTGAT CGGCGGCAAG 
GAGTACAAGA CGCCGGAGGT TCGATACCAG GTCATGCCCC ATGACCACCA ACACAAGCTG 
GCCAGCTTCT ACTACGCCGA CAAGAAGCTT ATCGAGAAGG CCATCAAAAC GGCGGTGGAA 
ACACAGCCCA AGTGGGACCG TGTGTCGATT GCAGACCGTC TGAAGATCTG GGAGAAGGCA 
GCGGACCTAA TGGCAACGAC GTACCGCCAG GATCTCAACG CCGCCACCAT GCTGGGTCAG 
TCAAAGACGG CGATTCAGGC GGAAATCGAT TCAGCAGCGG AGCTTATCGA CTTTATACGA 
ATGAATGCCT ATTTTCTGAA GGAGGTCACT AAATACCAGC CCATCAGCGA GAACATAAAG 
GTCACGAAGA ACTCCTTGCG CTATCGTGGC ATTGATGGCT TCATCGCCGC TGTCAGCCCC 
TTTAACTTTA CGGCCATCGG CGGCAACTTG TCCTACACGC CAGCTCTGAT GGGAAACGGC 
GTGCTGTGGA AGCCCTCGGA CACTGCCATG CTGTCCAACT GGATTATTTT CAAGATTATG 
CGCGAAGCGG GAGTGCCCGA TGGAGTCGTT AACTTTGTGC CCGCCGATGG GCCCGTGTTT 
GGAGACACGA TTACGGCCAG TCCCCACCTG GCAGGCATCA ATTTCACCGG CTCTGTGCCA 
ACCTTTAACC GCCTGTGGAA GCAGGTGGGC AACAACATTG ACAACTACGT GAACTTTCCT 
CGCTTGACTG GTGAGTGTGG CGGCAAGAAT TTCCACTTTA TACATGCATC AGCCGACGTT 
GAGTCAGTGG TGACCTCCAC GATACGCTCA GCATTTGAGT ACTGCGGCCA GAAGTGTTCC 
GCCTGCTCGC GAATGTATGT GCCGGAATCC CTTTGGCCAC AGATCAAGGA GGGCCTGGTA 
TGCGAGGCAG CAAAGCTGAA GATCGGTGAC GTTCAAGACT TCAGCAGCTT CACGTCGGCT 
GTGATTGATG ACAAAGCATT TAAACGCATT ACGGGCTACA TTGAGCATGC CAAAAAATCC 
CCGAACCTCG AGATTCTCGC CGGAGGCACT TACTCAGACA GCAAGGGCTA CTTCGTTAAT 
CCGACCATTG TACTCAGCAA GGATCCCAAG GACCGGATCA TGACCGAAGA GATTTTCGGT 
CCCGTGCTAT CTATTTATGT GTACAAGGAG TCGGACCTCC TTGAGACCAT GAAACTGGTG 
CACACCTCTA CCAAGTTTGC TTTAACCGGA GCAGTATTCG GGCAGGACGA GGATTTCGTC 
AAGTGCGCTC TGCAGGAGTT TAAAATGGCC GCTGGAAACT TCTACATCAA CGACAAGTCC 
ACTGGATCCG TGGTGGGCCA GCAGCCATTC GGAGGCGGCC GGATGTCCGG CACCAATGAC 
AAGGCCGGAG GACCACATTA TATTCTGCGC TGGACCTCTC CGCAGTCCAT CAAGGAGACC 
TTTGTGCCGC TGCGGGACGT CAACTATCCA TACATGTGCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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P5CDh1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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