CG32447 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG32447
Entrez Gene ID	40412
Full Name	CG32447 gene product from transcript CG32447-RD
Synonyms	CG14571,CG7155,Dmel\CG32447,anon-WO0170980.76,anon-WO0170980.77
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78E4-78E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_206422.2	NP_996144.1	uncharacterized protein, isoform B
NM_168935.2	NP_730679.2	uncharacterized protein, isoform C
NM_001275259.1	NP_001262188.1	uncharacterized protein, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG32447	NP_730679.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011646	XP_552620.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG32447 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG32447 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19229	NM_001275259.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG32447), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa21202	NM_168935.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG32447), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa21203	NM_206422.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG32447), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG32447 ( NM_001275259.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262188.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG32447), mRNA.

Price & Availability↑

CloneID	OFa19229
Clone ID Related Accession (Same CDS sequence)	NM_001275259.1
Accession Version	NM_001275259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGGTA GACGCCACCT GCTGCTGATC CTGCTGCTGA TCATGCAGTT GATCGCCACC 
ACGCCGGCGT CGCGCAGCCT CAGCGTTAAT GGCAACAACA TTTGGCGGCG GGTGCGCCTG 
GTGACCAGCC AGGAATCGGA TCGCAACGCC TACCAGGTGC TTAAGCCGAG TAGCCGTAAT 
GCCACCAGCA CCACCACTAC TACCAGCACG ACTACAAGTA CTACAAGTAG CACCACTAGT 
ACCACTACTC CGGCAGCTCC TCAGACCCAG CGATCTGCCA AACAATTGGA TCTCAATTTT 
CCGGGTGGCA ATGTCTCCAG TGCTGCCCGT CTCGAAATGT CGACGGAATT CTTTGTGGTA 
CCCACACTAT CGGCCAGCAG TTCTAGCAGC ACCACGTCCA CCACCACACC AGCCACGCCC 
CGTCGGAGTT CAGGGGCGAA GGCGCGAGCA GCCGGCACCA CTGGACGGGT GACTCCCGGG 
AGCAGCTCCA ACAGCACACC GCCCAGTATA GTGTCCACCC ACTTGCCCTT CGCGGGACTA 
CGCAAGGAGC CGTGGGTGGT GCCGGTGCTA GTCCTGGCCA CCCTCACCAT GCTCATGATG 
GCAGCGTTCG AGATCTTTGT GCTCTTCAAG GCCTGGCGGA CGTCCCCGTC GCGTAGGCAC 
CTCTTTCTGG GCCAGATGCT GCTTCTCGGT CTGTTCGCCT GCGCTAGTTT GGGGGCCATC 
ATCACCGCAC AGCCCTCGTT GATCAGTTGC GGGGCCATCC GCTTCGGAGT GGGCGTGGCC 
TACGCCCTGG TCTTTGCCGC CCTGCTGGTC AAATGCGTAT TTCTGATTAG TCTAAATGGA 
GGGGTGTATC TGCCGGCTCC ATACCAGGGA TTGCTGCTCC TCTTTGCGCT GCTCATCCAG 
GTAGCGATCG GAGGGCAGTG GCTGCTCACC CAACCACCGG AGGTATACAC CACCAGTGTG 
CCTGTGATGG GCAGTGGCTT CCTTAGCACC ACGGTGGCCT CACAGACCAA CTACTCGGCA 
CTGTTTTACC CCACGTCCTA CACAACGCTG GACGGGACTC CGGAGATCTA CACCCGGATA 
GCGGCGGTTA GCACCGTACT AATACCGCTC TGCAAGACGC AGTTCTCGGA GCTGCTCTTC 
TCGCTGATCT ACATTGTCTT TCTGATCGTC TTCATCGCCG TGCTAGCCAT CAAGTCGCGC 
GGCATCAGGG ACAACTACCG TGAGGCCACC TACATTGGGC TGGCCATCGG AGGAGCCATC 
CCCATCTGGC TGGGATGGAT GCTATGCGGC CTGGCCGTGG CCGAGCGGCA CAAGGACGCT 
TGCGTGGCCT TCGGTCTGGT AGCCACATCC GCCACGGTCT TCTTGGTGAT GTTCATGCCC 
AAGGGCAGGC AACTGGCGGC CATGGGCAAG GAGGGCCTGT ACGTGGAGGA CCGCGAGGAG 
CAGTTCAGTT CCCTGAGTCG CGCCGGTTCC GGCTACTCGC CCTCGTTCTT CCACTTCAAG 
CCCATCAAGT ACGGCGTGAT GAGCGGCTGC GGACTGCCCA ACTCTGCATC CAACACGGGC 
CAGGGACTCA GCTCCAAACA CTGCTCCAGT GCCAACAATG GAGGTGGCAT GTTTATACGA 
CCAGACGAAA CGAATCTTTA CACCACGCTG GAGCCCACGT TGAGCAGCAA TCCGAATGTG 
TACTTCCAGC GCAGCGGGGC CGTCCATCCC GGCATCCTGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262188.1
CDS	213..1937
Translation	MAGRRHLLLILLLIMQLIATTPASRSLSVNGNNIWRRVRLVTSQESDRNAYQVLKPSSRNATSTTTTTSTTTSTTSSTTSTTTPAAPQTQRSAKQLDLNFPGGNVSSAARLEMSTEFFVVPTLSASSSSSTTSTTTPATPRRSSGAKARAAGTTGRVTPGSSSNSTPPSIVSTHLPFAGLRKEPWVVPVLVLATLTMLMMAAFEIFVLFKAWRTSPSRRHLFLGQMLLLGLFACASLGAIITAQPSLISCGAIRFGVGVAYALVFAALLVKCVFLISLNGGVYLPAPYQGLLLLFALLIQVAIGGQWLLTQPPEVYTTSVPVMGSGFLSTTVASQTNYSALFYPTSYTTLDGTPEIYTRIAAVSTVLIPLCKTQFSELLFSLIYIVFLIVFIAVLAIKSRGIRDNYREATYIGLAIGGAIPIWLGWMLCGLAVAERHKDACVAFGLVATSATVFLVMFMPKGRQLAAMGKEGLYVEDREEQFSSLSRAGSGYSPSFFHFKPIKYGVMSGCGLPNSASNTGQGLSSKHCSSANNGGGMFIRPDETNLYTTLEPTLSSNPNVYFQRSGAVHPGILY

Target ORF information:

RefSeq Version	NM_001275259.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG32447), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275259.1

ORF Insert Sequence:

ATGGCCGGTA GACGCCACCT GCTGCTGATC CTGCTGCTGA TCATGCAGTT GATCGCCACC 
ACGCCGGCGT CGCGCAGCCT CAGCGTTAAT GGCAACAACA TTTGGCGGCG GGTGCGCCTG 
GTGACCAGCC AGGAATCGGA TCGCAACGCC TACCAGGTGC TTAAGCCGAG TAGCCGTAAT 
GCCACCAGCA CCACCACTAC TACCAGCACG ACTACAAGTA CTACAAGTAG CACCACTAGT 
ACCACTACTC CGGCAGCTCC TCAGACCCAG CGATCTGCCA AACAATTGGA TCTCAATTTT 
CCGGGTGGCA ATGTCTCCAG TGCTGCCCGT CTCGAAATGT CGACGGAATT CTTTGTGGTA 
CCCACACTAT CGGCCAGCAG TTCTAGCAGC ACCACGTCCA CCACCACACC AGCCACGCCC 
CGTCGGAGTT CAGGGGCGAA GGCGCGAGCA GCCGGCACCA CTGGACGGGT GACTCCCGGG 
AGCAGCTCCA ACAGCACACC GCCCAGTATA GTGTCCACCC ACTTGCCCTT CGCGGGACTA 
CGCAAGGAGC CGTGGGTGGT GCCGGTGCTA GTCCTGGCCA CCCTCACCAT GCTCATGATG 
GCAGCGTTCG AGATCTTTGT GCTCTTCAAG GCCTGGCGGA CGTCCCCGTC GCGTAGGCAC 
CTCTTTCTGG GCCAGATGCT GCTTCTCGGT CTGTTCGCCT GCGCTAGTTT GGGGGCCATC 
ATCACCGCAC AGCCCTCGTT GATCAGTTGC GGGGCCATCC GCTTCGGAGT GGGCGTGGCC 
TACGCCCTGG TCTTTGCCGC CCTGCTGGTC AAATGCGTAT TTCTGATTAG TCTAAATGGA 
GGGGTGTATC TGCCGGCTCC ATACCAGGGA TTGCTGCTCC TCTTTGCGCT GCTCATCCAG 
GTAGCGATCG GAGGGCAGTG GCTGCTCACC CAACCACCGG AGGTATACAC CACCAGTGTG 
CCTGTGATGG GCAGTGGCTT CCTTAGCACC ACGGTGGCCT CACAGACCAA CTACTCGGCA 
CTGTTTTACC CCACGTCCTA CACAACGCTG GACGGGACTC CGGAGATCTA CACCCGGATA 
GCGGCGGTTA GCACCGTACT AATACCGCTC TGCAAGACGC AGTTCTCGGA GCTGCTCTTC 
TCGCTGATCT ACATTGTCTT TCTGATCGTC TTCATCGCCG TGCTAGCCAT CAAGTCGCGC 
GGCATCAGGG ACAACTACCG TGAGGCCACC TACATTGGGC TGGCCATCGG AGGAGCCATC 
CCCATCTGGC TGGGATGGAT GCTATGCGGC CTGGCCGTGG CCGAGCGGCA CAAGGACGCT 
TGCGTGGCCT TCGGTCTGGT AGCCACATCC GCCACGGTCT TCTTGGTGAT GTTCATGCCC 
AAGGGCAGGC AACTGGCGGC CATGGGCAAG GAGGGCCTGT ACGTGGAGGA CCGCGAGGAG 
CAGTTCAGTT CCCTGAGTCG CGCCGGTTCC GGCTACTCGC CCTCGTTCTT CCACTTCAAG 
CCCATCAAGT ACGGCGTGAT GAGCGGCTGC GGACTGCCCA ACTCTGCATC CAACACGGGC 
CAGGGACTCA GCTCCAAACA CTGCTCCAGT GCCAACAATG GAGGTGGCAT GTTTATACGA 
CCAGACGAAA CGAATCTTTA CACCACGCTG GAGCCCACGT TGAGCAGCAA TCCGAATGTG 
TACTTCCAGC GCAGCGGGGC CGTCCATCCC GGCATCCTGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG32447 ( NM_168935.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730679.2 Drosophila melanogaster uncharacterized protein, transcript variant C (CG32447), mRNA.

Price & Availability↑

CloneID	OFa21202
Clone ID Related Accession (Same CDS sequence)	NM_168935.2
Accession Version	NM_168935.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168935.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGGTA GACGCCACCT GCTGCTGATC CTGCTGCTGA TCATGCAGTT GATCGCCACC 
ACGCCGGCGT CGCGCAGCCT CAGCGTTAAT GGCAACAACA TTTGGCGGCG GGTGCGCCTG 
GTGACCAGCC AGGAATCGGA TCGCAACGCC TACCAGGTGC TTAAGCCGAG TAGCCGTAAT 
GCCACCAGCA CCACCACTAC TACCAGCACG ACTACAAGTA CTACAAGTAG CACCACTAGT 
ACCACTACTC CGGCAGCTCC TCAGACCCAG CGATCTGCCA AACAATTGGA TCTCAATTTT 
CCGGGTGGCA ATGTCTCCAG TGCTGCCCGT CTCGAAATGT CGACGGAATT CTTTGTGGTA 
CCCACACTAT CGGCCAGCAG TTCTAGCAGC ACCACGTCCA CCACCACACC AGCCACGCCC 
CGTCGGAGTT CAGGGGCGAA GGCGCGAGCA GCCGGCACCA CTGGACGGGT GACTCCCGGG 
AGCAGCTCCA ACAGCACACC GCCCAGTATA GTGTCCACCC ACTTGCCCTT CGCGGGACTA 
CGCAAGGAGC CGTGGGTGGT GCCGGTGCTA GTCCTGGCCA CCCTCACCAT GCTCATGATG 
GCAGCGTTCG AGATCTTTGT GCTCTTCAAG GCCTGGCGGA CGTCCCCGTC GCGTAGGCAC 
CTCTTTCTGG GCCAGATGCT GCTTCTCGGT CTGTTCGCCT GCGCTAGTTT GGGGGCCATC 
ATCACCGCAC AGCCCTCGTT GATCAGTTGC GGGGCCATCC GCTTCGGAGT GGGCGTGGCC 
TACGCCCTGG TCTTTGCCGC CCTGCTGGTC AAATGCGTAT TTCTGATTAG TCTAAATGGA 
GGGGTGTATC TGCCGGCTCC ATACCAGGGA TTGCTGCTCC TCTTTGCGCT GCTCATCCAG 
GTAGCGATCG GAGGGCAGTG GCTGCTCACC CAACCACCGG AGGTATACAC CACCAGTGTG 
CCTGTGATGG GCAGTGGCTT CCTTAGCACC ACGGTGGCCT CACAGACCAA CTACTCGGCA 
CTGTTTTACC CCACGTCCTA CACAACGCTG GACGGGACTC CGGAGATCTA CACCCGGATA 
GCGGCGGTTA GCACCGTACT AATACCGCTC TGCAAGACGC AGTTCTCGGA GCTGCTCTTC 
TCGCTGATCT ACATTGTCTT TCTGATCGTC TTCATCGCCG TGCTAGCCAT CAAGTCGCGC 
GGCATCAGGG ACAACTACCG TGAGGCCACC TACATTGGGC TGGCCATCGG AGGAGCCATC 
CCCATCTGGC TGGGATGGAT GCTATGCGGC CTGGCCGTGG CCGAGCGGCA CAAGGACGCT 
TGCGTGGCCT TCGGTCTGGT AGCCACATCC GCCACGGTCT TCTTGGTGAT GTTCATGCCC 
AAGGGCAGGC AACTGGCGGC CATGGGCAAG GAGGGCCTGT ACGTGGAGGA CCGCGAGGAG 
CAGTTCAGTT CCCTGAGTCG CGCCGGTTCC GGCTACTCGC CCTCGTTCTT CCACTTCAAG 
CCCATCAAGT ACGGCGTGAT GAGCGGCTGC GGACTGCCCA ACTCTGCATC CAACACGGGC 
CAGGGACTCA GCTCCAAACA CTGCTCCAGT GCCAACAATG GAGGTGCACA CCTGAGTCCG 
CACCCGTTCT GCTATTATCC TCCCGCACCA CCACCACCAC TGCCACCGCA ACCACCGCCA 
CCACAGCAAG CCGCACCGCC CACGCTCACG CCGTTGACCC TCAAGCAGTT GGGCAACTCG 
CTAAGCTCCA TGACACAAGC GGCGCAGTTG GCCAAAACGC TGCGCTATGC GCCAGGCATG 
TTTATACGAC CAGACGAAAC GAATCTTTAC ACCACGCTGG AGCCCACGTT GAGCAGCAAT 
CCGAATGTGT ACTTCCAGCG CAGCGGGGCC GTCCATCCCG GCATCCTGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730679.2
CDS	213..2126
Translation	MAGRRHLLLILLLIMQLIATTPASRSLSVNGNNIWRRVRLVTSQESDRNAYQVLKPSSRNATSTTTTTSTTTSTTSSTTSTTTPAAPQTQRSAKQLDLNFPGGNVSSAARLEMSTEFFVVPTLSASSSSSTTSTTTPATPRRSSGAKARAAGTTGRVTPGSSSNSTPPSIVSTHLPFAGLRKEPWVVPVLVLATLTMLMMAAFEIFVLFKAWRTSPSRRHLFLGQMLLLGLFACASLGAIITAQPSLISCGAIRFGVGVAYALVFAALLVKCVFLISLNGGVYLPAPYQGLLLLFALLIQVAIGGQWLLTQPPEVYTTSVPVMGSGFLSTTVASQTNYSALFYPTSYTTLDGTPEIYTRIAAVSTVLIPLCKTQFSELLFSLIYIVFLIVFIAVLAIKSRGIRDNYREATYIGLAIGGAIPIWLGWMLCGLAVAERHKDACVAFGLVATSATVFLVMFMPKGRQLAAMGKEGLYVEDREEQFSSLSRAGSGYSPSFFHFKPIKYGVMSGCGLPNSASNTGQGLSSKHCSSANNGGAHLSPHPFCYYPPAPPPPLPPQPPPPQQAAPPTLTPLTLKQLGNSLSSMTQAAQLAKTLRYAPGMFIRPDETNLYTTLEPTLSSNPNVYFQRSGAVHPGILY

Target ORF information:

RefSeq Version	NM_168935.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG32447), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168935.2

ORF Insert Sequence:

ATGGCCGGTA GACGCCACCT GCTGCTGATC CTGCTGCTGA TCATGCAGTT GATCGCCACC 
ACGCCGGCGT CGCGCAGCCT CAGCGTTAAT GGCAACAACA TTTGGCGGCG GGTGCGCCTG 
GTGACCAGCC AGGAATCGGA TCGCAACGCC TACCAGGTGC TTAAGCCGAG TAGCCGTAAT 
GCCACCAGCA CCACCACTAC TACCAGCACG ACTACAAGTA CTACAAGTAG CACCACTAGT 
ACCACTACTC CGGCAGCTCC TCAGACCCAG CGATCTGCCA AACAATTGGA TCTCAATTTT 
CCGGGTGGCA ATGTCTCCAG TGCTGCCCGT CTCGAAATGT CGACGGAATT CTTTGTGGTA 
CCCACACTAT CGGCCAGCAG TTCTAGCAGC ACCACGTCCA CCACCACACC AGCCACGCCC 
CGTCGGAGTT CAGGGGCGAA GGCGCGAGCA GCCGGCACCA CTGGACGGGT GACTCCCGGG 
AGCAGCTCCA ACAGCACACC GCCCAGTATA GTGTCCACCC ACTTGCCCTT CGCGGGACTA 
CGCAAGGAGC CGTGGGTGGT GCCGGTGCTA GTCCTGGCCA CCCTCACCAT GCTCATGATG 
GCAGCGTTCG AGATCTTTGT GCTCTTCAAG GCCTGGCGGA CGTCCCCGTC GCGTAGGCAC 
CTCTTTCTGG GCCAGATGCT GCTTCTCGGT CTGTTCGCCT GCGCTAGTTT GGGGGCCATC 
ATCACCGCAC AGCCCTCGTT GATCAGTTGC GGGGCCATCC GCTTCGGAGT GGGCGTGGCC 
TACGCCCTGG TCTTTGCCGC CCTGCTGGTC AAATGCGTAT TTCTGATTAG TCTAAATGGA 
GGGGTGTATC TGCCGGCTCC ATACCAGGGA TTGCTGCTCC TCTTTGCGCT GCTCATCCAG 
GTAGCGATCG GAGGGCAGTG GCTGCTCACC CAACCACCGG AGGTATACAC CACCAGTGTG 
CCTGTGATGG GCAGTGGCTT CCTTAGCACC ACGGTGGCCT CACAGACCAA CTACTCGGCA 
CTGTTTTACC CCACGTCCTA CACAACGCTG GACGGGACTC CGGAGATCTA CACCCGGATA 
GCGGCGGTTA GCACCGTACT AATACCGCTC TGCAAGACGC AGTTCTCGGA GCTGCTCTTC 
TCGCTGATCT ACATTGTCTT TCTGATCGTC TTCATCGCCG TGCTAGCCAT CAAGTCGCGC 
GGCATCAGGG ACAACTACCG TGAGGCCACC TACATTGGGC TGGCCATCGG AGGAGCCATC 
CCCATCTGGC TGGGATGGAT GCTATGCGGC CTGGCCGTGG CCGAGCGGCA CAAGGACGCT 
TGCGTGGCCT TCGGTCTGGT AGCCACATCC GCCACGGTCT TCTTGGTGAT GTTCATGCCC 
AAGGGCAGGC AACTGGCGGC CATGGGCAAG GAGGGCCTGT ACGTGGAGGA CCGCGAGGAG 
CAGTTCAGTT CCCTGAGTCG CGCCGGTTCC GGCTACTCGC CCTCGTTCTT CCACTTCAAG 
CCCATCAAGT ACGGCGTGAT GAGCGGCTGC GGACTGCCCA ACTCTGCATC CAACACGGGC 
CAGGGACTCA GCTCCAAACA CTGCTCCAGT GCCAACAATG GAGGTGCACA CCTGAGTCCG 
CACCCGTTCT GCTATTATCC TCCCGCACCA CCACCACCAC TGCCACCGCA ACCACCGCCA 
CCACAGCAAG CCGCACCGCC CACGCTCACG CCGTTGACCC TCAAGCAGTT GGGCAACTCG 
CTAAGCTCCA TGACACAAGC GGCGCAGTTG GCCAAAACGC TGCGCTATGC GCCAGGCATG 
TTTATACGAC CAGACGAAAC GAATCTTTAC ACCACGCTGG AGCCCACGTT GAGCAGCAAT 
CCGAATGTGT ACTTCCAGCG CAGCGGGGCC GTCCATCCCG GCATCCTGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG32447 ( NM_206422.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996144.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG32447), mRNA.

Price & Availability↑

CloneID	OFa21203
Clone ID Related Accession (Same CDS sequence)	NM_206422.2
Accession Version	NM_206422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206422.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGGTA GACGCCACCT GCTGCTGATC CTGCTGCTGA TCATGCAGTT GATCGCCACC 
ACGCCGGCGT CGCGCAGCCT CAGCGTTAAT GGCAACAACA TTTGGCGGCG GGTGCGCCTG 
GTGACCAGCC AGGAATCGGA TCGCAACGCC TACCAGGTGC TTAAGCCGAG TAGCCGTAAT 
GCCACCAGCA CCACCACTAC TACCAGCACG ACTACAAGTA CTACAAGTAG CACCACTAGT 
ACCACTACTC CGGCAGCTCC TCAGACCCAG CGATCTGCCA AACAATTGGA TCTCAATTTT 
CCGGGTGGCA ATGTCTCCAG TGCTGCCCGT CTCGAAATGT CGACGGAATT CTTTGTGGTA 
CCCACACTAT CGGCCAGCAG TTCTAGCAGC ACCACGTCCA CCACCACACC AGCCACGCCC 
CGTCGGAGTT CAGGGGCGAA GGCGCGAGCA GCCGGCACCA CTGGACGGGT GACTCCCGGG 
AGCAGCTCCA ACAGCACACC GCCCAGTATA GTGTCCACCC ACTTGCCCTT CGCGGGACTA 
CGCAAGGAGC CGTGGGTGGT GCCGGTGCTA GTCCTGGCCA CCCTCACCAT GCTCATGATG 
GCAGCGTTCG AGATCTTTGT GCTCTTCAAG GCCTGGCGGA CGTCCCCGTC GCGTAGGCAC 
CTCTTTCTGG GCCAGATGCT GCTTCTCGGT CTGTTCGCCT GCGCTAGTTT GGGGGCCATC 
ATCACCGCAC AGCCCTCGTT GATCAGTTGC GGGGCCATCC GCTTCGGAGT GGGCGTGGCC 
TACGCCCTGG TCTTTGCCGC CCTGCTGGTC AAATGCGTAT TTCTGATTAG TCTAAATGGA 
GGGGTGTATC TGCCGGCTCC ATACCAGGGA TTGCTGCTCC TCTTTGCGCT GCTCATCCAG 
GTAGCGATCG GAGGGCAGTG GCTGCTCACC CAACCACCGG AGGTATACAC CACCAGTGTG 
CCTGTGATGG GCAGTGGCTT CCTTAGCACC ACGGTGGCCT CACAGACCAA CTACTCGGCA 
CTGTTTTACC CCACGTCCTA CACAACGCTG GACGGGACTC CGGAGATCTA CACCCGGATA 
GCGGCGGTTA GCACCGTACT AATACCGCTC TGCAAGACGC AGTTCTCGGA GCTGCTCTTC 
TCGCTGATCT ACATTGTCTT TCTGATCGTC TTCATCGCCG TGCTAGCCAT CAAGTCGCGC 
GGCATCAGGG ACAACTACCG TGAGGCCACC TACATTGGGC TGGCCATCGG AGGAGCCATC 
CCCATCTGGC TGGGATGGAT GCTATGCGGC CTGGCCGTGG CCGAGCGGCA CAAGGACGCT 
TGCGTGGCCT TCGGTCTGGT AGCCACATCC GCCACGGTCT TCTTGGTGAT GTTCATGCCC 
AAGGGCAGGC AACTGGCGGC CATGGGCAAG GAGGGCCTGT ACGTGGAGGA CCGCGAGGAG 
CAGTTCAGTT CCCTGAGTCG CGCCGGTTCC GGCTACTCGC CCTCGTTCTT CCACTTCAAG 
CCCATCAAGT ACGGCGTGAT GAGCGGCTGC GGACTGCCCA ACTCTGCATC CAACACGGGC 
CAGGGACTCA GCTCCAAACA CTGCTCCAGT GCCAACAATG GAGGTGATCG GGTTGCTCTG 
GTCACTGCCG CACCGCCGAG CTATACGCGT ATGTATCACT ATTTTCCAGC ACACCTGAGT 
CCGCACCCGT TCTGCTATTA TCCTCCCGCA CCACCACCAC CACTGCCACC GCAACCACCG 
CCACCACAGC AAGCCGCACC GCCCACGCTC ACGCCGTTGA CCCTCAAGCA GTTGGGCAAC 
TCGCTAAGCT CCATGACACA AGCGGCGCAG TTGGCCAAAA CGCTGCGCTA TGCGCCAGGC 
ATGTTTATAC GACCAGACGA AACGAATCTT TACACCACGC TGGAGCCCAC GTTGAGCAGC 
AATCCGAATG TGTACTTCCA GCGCAGCGGG GCCGTCCATC CCGGCATCCT GTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996144.1
CDS	213..2189
Translation	MAGRRHLLLILLLIMQLIATTPASRSLSVNGNNIWRRVRLVTSQESDRNAYQVLKPSSRNATSTTTTTSTTTSTTSSTTSTTTPAAPQTQRSAKQLDLNFPGGNVSSAARLEMSTEFFVVPTLSASSSSSTTSTTTPATPRRSSGAKARAAGTTGRVTPGSSSNSTPPSIVSTHLPFAGLRKEPWVVPVLVLATLTMLMMAAFEIFVLFKAWRTSPSRRHLFLGQMLLLGLFACASLGAIITAQPSLISCGAIRFGVGVAYALVFAALLVKCVFLISLNGGVYLPAPYQGLLLLFALLIQVAIGGQWLLTQPPEVYTTSVPVMGSGFLSTTVASQTNYSALFYPTSYTTLDGTPEIYTRIAAVSTVLIPLCKTQFSELLFSLIYIVFLIVFIAVLAIKSRGIRDNYREATYIGLAIGGAIPIWLGWMLCGLAVAERHKDACVAFGLVATSATVFLVMFMPKGRQLAAMGKEGLYVEDREEQFSSLSRAGSGYSPSFFHFKPIKYGVMSGCGLPNSASNTGQGLSSKHCSSANNGGDRVALVTAAPPSYTRMYHYFPAHLSPHPFCYYPPAPPPPLPPQPPPPQQAAPPTLTPLTLKQLGNSLSSMTQAAQLAKTLRYAPGMFIRPDETNLYTTLEPTLSSNPNVYFQRSGAVHPGILY

Target ORF information:

RefSeq Version	NM_206422.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG32447), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206422.2

ORF Insert Sequence:

ATGGCCGGTA GACGCCACCT GCTGCTGATC CTGCTGCTGA TCATGCAGTT GATCGCCACC 
ACGCCGGCGT CGCGCAGCCT CAGCGTTAAT GGCAACAACA TTTGGCGGCG GGTGCGCCTG 
GTGACCAGCC AGGAATCGGA TCGCAACGCC TACCAGGTGC TTAAGCCGAG TAGCCGTAAT 
GCCACCAGCA CCACCACTAC TACCAGCACG ACTACAAGTA CTACAAGTAG CACCACTAGT 
ACCACTACTC CGGCAGCTCC TCAGACCCAG CGATCTGCCA AACAATTGGA TCTCAATTTT 
CCGGGTGGCA ATGTCTCCAG TGCTGCCCGT CTCGAAATGT CGACGGAATT CTTTGTGGTA 
CCCACACTAT CGGCCAGCAG TTCTAGCAGC ACCACGTCCA CCACCACACC AGCCACGCCC 
CGTCGGAGTT CAGGGGCGAA GGCGCGAGCA GCCGGCACCA CTGGACGGGT GACTCCCGGG 
AGCAGCTCCA ACAGCACACC GCCCAGTATA GTGTCCACCC ACTTGCCCTT CGCGGGACTA 
CGCAAGGAGC CGTGGGTGGT GCCGGTGCTA GTCCTGGCCA CCCTCACCAT GCTCATGATG 
GCAGCGTTCG AGATCTTTGT GCTCTTCAAG GCCTGGCGGA CGTCCCCGTC GCGTAGGCAC 
CTCTTTCTGG GCCAGATGCT GCTTCTCGGT CTGTTCGCCT GCGCTAGTTT GGGGGCCATC 
ATCACCGCAC AGCCCTCGTT GATCAGTTGC GGGGCCATCC GCTTCGGAGT GGGCGTGGCC 
TACGCCCTGG TCTTTGCCGC CCTGCTGGTC AAATGCGTAT TTCTGATTAG TCTAAATGGA 
GGGGTGTATC TGCCGGCTCC ATACCAGGGA TTGCTGCTCC TCTTTGCGCT GCTCATCCAG 
GTAGCGATCG GAGGGCAGTG GCTGCTCACC CAACCACCGG AGGTATACAC CACCAGTGTG 
CCTGTGATGG GCAGTGGCTT CCTTAGCACC ACGGTGGCCT CACAGACCAA CTACTCGGCA 
CTGTTTTACC CCACGTCCTA CACAACGCTG GACGGGACTC CGGAGATCTA CACCCGGATA 
GCGGCGGTTA GCACCGTACT AATACCGCTC TGCAAGACGC AGTTCTCGGA GCTGCTCTTC 
TCGCTGATCT ACATTGTCTT TCTGATCGTC TTCATCGCCG TGCTAGCCAT CAAGTCGCGC 
GGCATCAGGG ACAACTACCG TGAGGCCACC TACATTGGGC TGGCCATCGG AGGAGCCATC 
CCCATCTGGC TGGGATGGAT GCTATGCGGC CTGGCCGTGG CCGAGCGGCA CAAGGACGCT 
TGCGTGGCCT TCGGTCTGGT AGCCACATCC GCCACGGTCT TCTTGGTGAT GTTCATGCCC 
AAGGGCAGGC AACTGGCGGC CATGGGCAAG GAGGGCCTGT ACGTGGAGGA CCGCGAGGAG 
CAGTTCAGTT CCCTGAGTCG CGCCGGTTCC GGCTACTCGC CCTCGTTCTT CCACTTCAAG 
CCCATCAAGT ACGGCGTGAT GAGCGGCTGC GGACTGCCCA ACTCTGCATC CAACACGGGC 
CAGGGACTCA GCTCCAAACA CTGCTCCAGT GCCAACAATG GAGGTGATCG GGTTGCTCTG 
GTCACTGCCG CACCGCCGAG CTATACGCGT ATGTATCACT ATTTTCCAGC ACACCTGAGT 
CCGCACCCGT TCTGCTATTA TCCTCCCGCA CCACCACCAC CACTGCCACC GCAACCACCG 
CCACCACAGC AAGCCGCACC GCCCACGCTC ACGCCGTTGA CCCTCAAGCA GTTGGGCAAC 
TCGCTAAGCT CCATGACACA AGCGGCGCAG TTGGCCAAAA CGCTGCGCTA TGCGCCAGGC 
ATGTTTATAC GACCAGACGA AACGAATCTT TACACCACGC TGGAGCCCAC GTTGAGCAGC 
AATCCGAATG TGTACTTCCA GCGCAGCGGG GCCGTCCATC CCGGCATCCT GTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG32447 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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