Wnk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wnk
Entrez Gene ID	40391
Full Name	Wnk kinase
Synonyms	CG7177,DWNK,Dmel\CG7177,WNK,cg7177,dWNK,wnk
General protein information	Preferred Names Wnk kinase Names CG7177 gene product from transcript CG7177-RM CG7177-PL CG7177-PM CG7177-PN CG7177-PO WNK homolog WNK kinase Wnk-PL Wnk-PM Wnk-PN Wnk-PO with-no-lysine kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78D5-78D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275242.2	NP_001262171.2	Wnk kinase, isoform M
NM_001275243.3	NP_001262172.3	Wnk kinase, isoform N
NM_001275244.2	NP_001262173.2	Wnk kinase, isoform O
NM_001275245.3	NP_001262174.3	Wnk kinase, isoform L

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Drosophila WNK-SPAK/OSR1-NKCC cascade is an essential molecular pathway for osmoregulation.
Title: Hypotonicity stimulates potassium flux through the WNK-SPAK/OSR1 kinase cascade and the Ncc69 sodium-potassium-2-chloride cotransporter in the Drosophila renal tubule.

Drosophila WNK mutant caused defects in axon guidance during embryogenesis.
Title: WNK signaling is involved in neural development via Lhx8/Awh expression.

The following Wnk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wnk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa41367	NM_001275243.3 Latest version!	Drosophila melanogaster Wnk kinase (Wnk), transcript variant N, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OFa41367	NM_001275245.3 Latest version!	Drosophila melanogaster Wnk kinase (Wnk), transcript variant L, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OFa41368	NM_001275242.2 Latest version!	Drosophila melanogaster Wnk kinase (Wnk), transcript variant M, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OFa41369	NM_001275244.2 Latest version!	Drosophila melanogaster Wnk kinase (Wnk), transcript variant O, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wnk ( NM_001275243.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262172.3 Drosophila melanogaster Wnk kinase (Wnk), transcript variant N, mRNA.

Price & Availability↑

CloneID	OFa41367
Clone ID Related Accession (Same CDS sequence)	NM_001275243.3 , NM_001275245.3
Accession Version	NM_001275243.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Wnk kinase, isoform N
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Oct 19, 2015 this sequence version replaced NM_001275243.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATTGGACAAA CCCTCTGCGA CTTCGTACGA CAACAAGAGG TCGTACCTGC GAACCGTGCT 
CGGAAGGGAA GCGCCATCAG CGAAGATGGT ACTACCTCCT GCACCACGCA ACCTCAAAGG 
AATACCTCAA TTTCTAGCGA GGAGCAAACT GTGCAGGGAG CTAATATACA AAAGTCGGGA 
CACCGTTCGT TTAATAGATC TGCCTCCTCT AGGCAAAAGC CGAACCCGAC ATCAAAACTA 
AAAGACACAC TAAACAGAAA GCCCACTTGC AAGCAGGGCA CACTTTCTGC ACACACGAGC 
ACTAGCAGCA CCACTTCAAT CCAGTCATCA CCTATCGAGC CAGCTTCTTC TCTACCCACG 
CTGAACACCA CGCCTACACC ACCAGCTGCC AGCAAAAGTA ACCTATTTGT GCGGGTGCTA 
AGACGCTTTT CAAATAACAC CCTTCCCGGC AAAACGGCGT CAAGCCCGAA AAACGTGGAC 
GACGTACCGA AAGTCAACGA TAACAACAAC GGCGATTTAG GCAAACAGCA ATCAACCGAT 
GTAGAGGTGC TTTGCTCTCA GGACAACCAG AGCAGGGAAC AGAATGAGGA CGAAATGGAT 
AGCAAAATAG AACCTGCAGA TGCCATAAGC AACCAGAAAG CTGGTCTTAC TTCGGGCAAA 
CCTAAAAAAG ACAAGTCAGT CAAGGGGACG TCGCAAGCGG TTGTTTCAGA CACCAAGAAA 
ATGGAGGCCA GAAAGTCCTC CTCGGACACG GTGATCGAGC CATTGATCAA GCAGCAGGCG 
CCACTGCACT CGTCAAAGAC CACGCCAACG ACTTTGACGG CGAACTTCGT GCAAAACATT 
CGATTCGTCC GTAAAAATGT GGAACAGTCA GGCAGGAACA CCAATCCACT GCAGTTCGTT 
GAACTCGAGA CAGAGTTTCC TCGTGACTAT GACGACAACA TCGAGATGCT TTCCCGCGAG 
GCGGAGCATC TGGAGGAGCA GTTCCGCACG CCTACGCGCT CCAATGCTAC GGATGCCACT 
TCCCACCATG TGGCCGGAAT CATCGACAAC ATTATAACCG AAGCGTCACG GAGTCTTACA 
ATCGAAAAGA CTGAAGACGA TGTGCCATTG AAGTCTTCTA CCAAGCACTC AAGCGGCGTA 
AAACGAGTTG GCTTTCAAGT AGAGGATAAG GACGATACCG AAGTTCAGAG CGAAAAGCAG 
GCAAAGAGCT TCGACGACAT AACCAAAAAA GCGGAGAGCT CCGAGGCTAG CGCAGAGGAA 
GCTGCTGTCA CCGGGTCCTC GACAGATGCA TCTGCATCTC CCCTGCCCTC CACGTCGCTG 
GTCTCCACCA CATCTTCAGC TACATCGATA ACCAAGAGTA AGAGCGATGA GGACGATGAT 
CCGGTGGCCA TGTCGCCGTG CGGACGATTC TTTAAGTACG ATAAGGAAGT CGGACGCGGT 
TCCTTTAAAA CTGTCTATCG TGGCCTGGAC ACGTTGACTG GTGTTCCAGT TGCCTGGTGC 
GAGTTATTGG ACAAGCAAGT AAAAAAAAGT GAACGTACTA GATTTCGCGA GGAGGCAGAC 
ATGCTGAAGA AACTTCAGCA CCCAAACATA GTGCGGTTTT ATACATATTG GGAGTTTCCT 
ATTGGCCGCA AGAAGAATAT TGTACTAGTC ACCGAACTAA TGTTATCTGG AACTTTGAAA 
TCCTATTTGA AACGATTCAA AAAAATACAC CCAAAGGTAT TGAAGTCATG GTGTCGCCAG 
ATACTAAAAG GCCTTAACTT CCTGCATACT CGTCAATTTC CTATAATTCA TCGTGATTTG 
AAATGTGATA ATATTTTTAT AACTGGCACC ACTGGTAGTG TTAAAATCGG CGACTTGGGC 
CTGGCAACTT TGAAAAACCG CTCCCACGCG AAATCTGTGA TTGGTACGCC GGAGTTTATG 
GCCCCGGAAA TGTACGAGGA GCACTACGAC GAGTCGGTGG ACGTATATGC ATTCGGCATG 
TGCATGTTGG AGATGGCCAT TTCCGAATAT CCTTACAGTG AGTGCAAGGG TCCTGCACAG 
ATCTACAAAA AGGTGATATC GGGCATTAAG CCAGCGGCGT TGGCTAAGGT GGAGGATCCC 
AACGTGCGCG ACATTATCGA ACGGTGCATT GAACTTAAAA AGGAGGATCG CCCCAGCTGC 
AATGAACTGC TAGAATCGGA GTTCTTTGAC GAAGATATCG GTATACGAGT GGAGCCAACT 
GCTTCGGAGC AGTTCCTTTC TGATCCGAGC ATCAGCATCA TCGAGTTCCG ACTGCGGTTT 
ATGGATCCCA AGAAGCGCTC GTCCCGTCAT AAGGAAAACG AGGCAATTCA GTTTGAGTAC 
AACATCAGGC ATGACGAGTA CGAACAGATT GCGCAAGAAA TGATGAAGGA AAATATTATA 
TCTGAGGACG ACTCTCGCGC TGTAGCTCGG CTTTTAAAAG TGCAGGTGGT GTCGTTGCTT 
AAGGAACGTG CCCAGCGTCA AACTCAAATC AAGCTGCAAA ACGAGAAGTC TCGTTTGGAA 
AAATTGGCAT TGCAAAAGCA ACGTGAGAGT TTGCCGACTA ACGTCGATGA AGACGAGGAA 
GAGGAGGAGG AGTCCGAGGA CGAGGAGGAT GGTGTTAAAT GGAATCAGCG CCTGCAGCTA 
AGATATGACC TGCTTAACAC TGATTCAGAA ACAAGCCTGG CTCTATCCAC GAACAGCGTC 
GAACCGCAGC AGCTGTCCAC TCGCTCGAAC ACTTCGATTC CGAACAGTGG CATTCAGCAA 
CCTGTTCAGG TCCCAGGTCA AGTGCCTGTT TCCCAGTTGA TCTCGGTGCA ACCACAGGCT 
ATTCCGTCAC CGGCTATTCC CATGCAACAG AAGCCGACGG TGCACTACAT TCAACCGCCA 
CAGCTGGCCT CTTATCAAAA TTCGAATGCA TCGATGCAGG AGATGACCAA TAACCAGGTC 
ATTTCGCCGA CAGGCAGTCA ACAAATGCAA CAGCAACAAC CCGTAGTGGC ACCAACAGTC 
AATCATCAGG TCATGCCGCA GCAGCAAGTG AACCAACAAC AGCAACAACC ACAAATGATG 
CAGCAAATAC CCCAGCAAGT TCAAGTGCAA CAGCCCCAAA CTGTATTGCC TCCGCAGCCA 
CATGAGCAGC AGCCACAGCA ACAACAACAG CCACTGCAGC AACAACTGCA GCAGCTTATG 
CATACTAACG TGCAGGCTCC GGATTTGACT CAACAGCAAC AAATGGCTCA GCAACAGGCC 
CAGCAATATT TTCAGCAGCA GCAGCAACAG CCTCAGCAGG CCGTTAACAT GCAACAAGCT 
TATGCAATGC AGCAAGCTGG CCAACAGCAA CAGCTTTCGC AGCCTCTTCA AATTCAGCAA 
CAGATCTTGC AGCAACAACA AGTGGCCGTT TCACATCAGC AACAGATAAT GCAGCAACAA 
CTGGCCCAGC ATCAGCTGCA ACAGCTGCAG CAGCAGCAGC TTCAACAACA GCAACTGCAA 
CAGCAGCAGC AAATACAACA ACAGCAACTC CAACAACAAC AACTCCAGCA ACAGCAATTC 
GTACAGCAGT ATGCGCAGGC AATGCCTCAG CAACAACATC AGCAACTCGT TACGGGATCG 
CAAGTCATGG CACCGCATCA ACATCAGCAA CCTATTCAGA TCCCAGTGCA GATGCAAGTT 
CCACCGACGT CCGTGGCTCC TCCAATACAA CACACTTACA ATCAGCAGGG CGGACAAGTA 
ACGCTCAGTG ATGCCCAACA GCAACAGCAT CCTGGATTCT CGGCCGTACC ACAACAGGCA 
GCGCCATTTA TACAACAGCC CACACAGCAA CCCATTCAGC TATCAATGCC TTTGGAACAA 
CAATTGCAGC AATTGCTGCA TAGCCAGCCA GCCCAGCAAC AACAGGCCAT GAGCCAACAG 
CAGCAACAGC CCTTGGTGCA ACAGCAACAA CTGCCTTTGG TACAACAGCA GCCGCCTTTG 
GTCCAGCAGC AGCAACCGCT GGTTCAGCAT CAGCAGCCTT CGGTCCAACA TCAGCAACCT 
TTGGTGCAAC AGCCCCAGCA ACAGCAACCA CAGCCCCAGA ATCAGCAACC ACAGCCCCAG 
ACACAGCAAA CACACGTGGT TCAGCAACAA CCACCACAGC AGCAACCTGC AGTAGAGCAA 
ATCTCACAGA TTTCTTCGCA AGTGCCTGTT CAACAGGAAA ATCTGCAGCC AATACAGGTC 
AACAAAGATG CGAATGTCGC AACGGATGCA ATGTCCCTAA ATAGCGCCCA CGGTGCCCTC 
GAACCTGCAC CGAAGACCGA ACCCCAGAAC TCTGCTGATG CGGAAAAGCA GCAAAAGCAG 
ACAGGCACGG GCACACGTTC CCAGAAACCG AGGCGATCAA ACCGGAGCGG CAACGAACGG 
ATTCCCAAAC TTAGTGTAAC CAGCGTCGAC GAGGGCAGCG TCATAAACTG TCACATGGAA 
AATAAACTTA AAACGATTAC ATTTAAATTC GACATAGGAG ACGTAAATCC GGTTGAAATA 
GCCAATAAAT TGATCGCCCA AGATTTGCTT TCTAATTGTC AAAGTACAGT TTTTGTGGAA 
ATGATCAATG AAATTGTTGA CCAAGTCAAA CAGAATCCCA ATCAAATCCC TATACCGACA 
AATTATCGCC GCAATATTGA AAAGCGGGAT GAAACCGCCA GCGACATCAC AAAGATGTTC 
GAGCCCACTA TCCATGGTGT TGAAACCCTG CCATCCGGTG GAGGAGGCGC TGAACAGTCA 
AACTGCAACT TGACTTTAGA GGCCAGGTCC CATTTATCCA ACATACCAAA TGCGAAAGAA 
CCACAGCTGA ACGTCAGCAC TCCGCCCACA ACCACTTCGA CTATGTCTTC CTCATCAACC 
GCATCGAGGG ATGCGCCCAA TAGTAGCAAT GACGTGACCA TCGGCTCTGG TTCCGTTTCT 
CGAAAAACGA GCACGGCCTC GGAGTACACT TCGCTATCGA TTGACTATAT GCCCGACAGC 
AACATTACTC CAACTGGTCC GGAGCCACCG CTTGACGATG GCAAGGATAA AAAACCTTGC 
TCCCTTGCTA TAGCCAGAAT GCAAAAGCTC TTGGAGTCGG CCGGCAATGG TGCTCCTCGC 
AGCCTGAATT TGCCCTTGAA TCGTCACCTG AAAATTCAAG AGGACTTGAA GCATACTAGA 
AGTCTTGACG ATTTGACCGC AGTGAAAATA ACCTTTGACA TGCCCAACAA GGCTGCATTG 
GACACTTCGG AAAACGCTCA GCAGGCCACA GAAGCGGAAG CGGAGAAACC AAAAGATAAA 
TCCGGACAGG CAGTAGGCAA CCAGGGAACT GGAGCGGCCA ACACTCTAGA GCAGTTAAAA 
ATAGAACTGG AGAACATTAC TCATGCCCAC GCGTTTGCCT CCGCTGTAGT GGCCTCGATT 
AACAACAGAG CTCCTCATCA AGCTTCCCCA GCAATGTCCT CCCTAAAGGC CACTTCTGGA 
CAACCACAGA CACAAGAGAT AACCAAGCCA AATAACGGAG CAGGCCCTTC AGTACCCTCA 
GTGGGTCAGA ATACTCCCAC TGCAGCCTTA ACATCCGCCA GGGGCTCAGG CTCCTCCGTC 
TACAACTCAC GTCGCACATC CATCGACAAC AGCGTGGGAT CCGACATGCA CCTGCACACT 
GCCACTAATC TTGAAGGGAC AGTGAGCAAC CCGGATCCGA TCCCGACTGA GGCATCGGTT 
GGAATTACCA TTGCTACGGG TCATGAGAAG CAACTCTCCA AGCAGCCCAG TTTGGAAAAG 
CCTTCTGCCA CTTCCATTTT AACGAACAAC AGTGATCCTC CGCAAAGGAA TCCCAGCAAT 
GGATCAATTA ACCAAAATTC AATAGCTGAT TTGGAAAAGA AATTGGCAGC TTTGCGAAAT 
ACCGAAAACA CAGAAGAGTC TGCAACAGCA ACGTTGTCAG TGGTTCCTAA ACAAGTTGAA 
GAGGTAGTCA ATCCAAGCGC GCGTAAAATT TCGCGTTTTA GTGTCAGTCG TGTGCAGGAG 
CAGAAAACTT CAACCGGAGT TGAGGAACCA GCTCAAGGTC AACTTAAGAT CGACCTCCAA 
GTCGCCGGCC CTGGAGGCCA GGTGCAGACC AACAACTCCG TTCAGAACGG CAGCGTGGTC 
AACACTCCAA CCGAAGTCAT CAGCTCTCCT ATTCAAAATG TTCCGCTGGC CATTAACGGA 
ATTCAGTTGA TGTACCAACA GCCCCAGCAG ATACACCAGT TGCCAGGTGG AACATCAACT 
ACTACAAGTG GAGCAGGGAC TCAGTGCATT GTAGTGCCGC AAGTGGTCAA TCAAAATGGC 
ACCCACGTGC AACAGCCGTC TAACCTACAG CCACAGCAGC AGAGCGTACA TCCAAATATG 
ACTCAACAGC CACAGCAGAC GCCACTTAAT GGGCACCCAT CTATGGTTAA CACTCTTCAG 
CAGCAGCCAC CACAACAATC CTTACCGATG CAGACGATTC AGTCGCAGCA GCAGCAGCAT 
AACCAAATGC CTATCATTTC GCAACAACAG CAGCAGCAGA TCCTAATGCA GCAGCAACAG 
CAACAGGGAT CCCAGCAAGG ATCACAGCAA TTTAATTTGC CTGGCACACA ACAGACTCAT 
CCTCAGCACC AGTTCATCCA GTCGCAGCCC AACCAACTTC ACTCGCTTCC GCCCCAGGTA 
GTGAGCATGG CTCAGGGAAA TCTGCCGCAT CAGCTTCCAC CCATGCAGAC TATGGGAGCC 
CAACAGCAAA TGATCTCGCA ACAACAGCAT CAGTTGCAGA TGCAGTCGCA TATGCACCAA 
CAAAACGTGC CCGGAATGTA CAACCAGCAG ACTGGAGCTC GTGTGGCAGC ACCCCAAAAT 
TTCGCAGGTG CAGTTCCAAA TCATTTGTTG CAGCAGTCCC CTTTGATGGC ATCCCAGCAG 
CCAGCACAGC CCATGCAACA CGTTATGCAA CCGATTTTCA ATGCAACGTC CGGTGAAGTT 
ACCGAAGAGC CTGTCTCTTT AGCAGCTACG CATCCGCATC TGCTACCGAG CGATATTCAA 
TCCGATATAA AGCACAATTT AGACTCATTA GTGAACCAAT TGTGCAATAC GCGCTTGGGT 
ACTAACCAGC ACCAACGGCT GTTGCTGTTG CGTCAGAGAC AACTCATCGA AGAGGACGAG 
CTGCGTCTTA AACATTATGT GGAATACGAA AAGTTCCAAA AGGCACTGCG CCAATCTATT 
AGCACAAACG TTCCAGCTAA TGCAGCTTAC TATTCTGCGG CTGCCGCACA GCTTCCAACT 
AATTTGGCAG CCCCAGCAGC TCCGTCCTCA TCGAATCCGA CGTCCACTAG CTCCGCAAAC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262172.3
CDS	586..7911
Translation	IGQTLCDFVRQQEVVPANRARKGSAISEDGTTSCTTQPQRNTSISSEEQTVQGANIQKSGHRSFNRSASSRQKPNPTSKLKDTLNRKPTCKQGTLSAHTSTSSTTSIQSSPIEPASSLPTLNTTPTPPAASKSNLFVRVLRRFSNNTLPGKTASSPKNVDDVPKVNDNNNGDLGKQQSTDVEVLCSQDNQSREQNEDEMDSKIEPADAISNQKAGLTSGKPKKDKSVKGTSQAVVSDTKKMEARKSSSDTVIEPLIKQQAPLHSSKTTPTTLTANFVQNIRFVRKNVEQSGRNTNPLQFVELETEFPRDYDDNIEMLSREAEHLEEQFRTPTRSNATDATSHHVAGIIDNIITEASRSLTIEKTEDDVPLKSSTKHSSGVKRVGFQVEDKDDTEVQSEKQAKSFDDITKKAESSEASAEEAAVTGSSTDASASPLPSTSLVSTTSSATSITKSKSDEDDDPVAMSPCGRFFKYDKEVGRGSFKTVYRGLDTLTGVPVAWCELLDKQVKKSERTRFREEADMLKKLQHPNIVRFYTYWEFPIGRKKNIVLVTELMLSGTLKSYLKRFKKIHPKVLKSWCRQILKGLNFLHTRQFPIIHRDLKCDNIFITGTTGSVKIGDLGLATLKNRSHAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMAISEYPYSECKGPAQIYKKVISGIKPAALAKVEDPNVRDIIERCIELKKEDRPSCNELLESEFFDEDIGIRVEPTASEQFLSDPSISIIEFRLRFMDPKKRSSRHKENEAIQFEYNIRHDEYEQIAQEMMKENIISEDDSRAVARLLKVQVVSLLKERAQRQTQIKLQNEKSRLEKLALQKQRESLPTNVDEDEEEEEESEDEEDGVKWNQRLQLRYDLLNTDSETSLALSTNSVEPQQLSTRSNTSIPNSGIQQPVQVPGQVPVSQLISVQPQAIPSPAIPMQQKPTVHYIQPPQLASYQNSNASMQEMTNNQVISPTGSQQMQQQQPVVAPTVNHQVMPQQQVNQQQQQPQMMQQIPQQVQVQQPQTVLPPQPHEQQPQQQQQPLQQQLQQLMHTNVQAPDLTQQQQMAQQQAQQYFQQQQQQPQQAVNMQQAYAMQQAGQQQQLSQPLQIQQQILQQQQVAVSHQQQIMQQQLAQHQLQQLQQQQLQQQQLQQQQQIQQQQLQQQQLQQQQFVQQYAQAMPQQQHQQLVTGSQVMAPHQHQQPIQIPVQMQVPPTSVAPPIQHTYNQQGGQVTLSDAQQQQHPGFSAVPQQAAPFIQQPTQQPIQLSMPLEQQLQQLLHSQPAQQQQAMSQQQQQPLVQQQQLPLVQQQPPLVQQQQPLVQHQQPSVQHQQPLVQQPQQQQPQPQNQQPQPQTQQTHVVQQQPPQQQPAVEQISQISSQVPVQQENLQPIQVNKDANVATDAMSLNSAHGALEPAPKTEPQNSADAEKQQKQTGTGTRSQKPRRSNRSGNERIPKLSVTSVDEGSVINCHMENKLKTITFKFDIGDVNPVEIANKLIAQDLLSNCQSTVFVEMINEIVDQVKQNPNQIPIPTNYRRNIEKRDETASDITKMFEPTIHGVETLPSGGGGAEQSNCNLTLEARSHLSNIPNAKEPQLNVSTPPTTTSTMSSSSTASRDAPNSSNDVTIGSGSVSRKTSTASEYTSLSIDYMPDSNITPTGPEPPLDDGKDKKPCSLAIARMQKLLESAGNGAPRSLNLPLNRHLKIQEDLKHTRSLDDLTAVKITFDMPNKAALDTSENAQQATEAEAEKPKDKSGQAVGNQGTGAANTLEQLKIELENITHAHAFASAVVASINNRAPHQASPAMSSLKATSGQPQTQEITKPNNGAGPSVPSVGQNTPTAALTSARGSGSSVYNSRRTSIDNSVGSDMHLHTATNLEGTVSNPDPIPTEASVGITIATGHEKQLSKQPSLEKPSATSILTNNSDPPQRNPSNGSINQNSIADLEKKLAALRNTENTEESATATLSVVPKQVEEVVNPSARKISRFSVSRVQEQKTSTGVEEPAQGQLKIDLQVAGPGGQVQTNNSVQNGSVVNTPTEVISSPIQNVPLAINGIQLMYQQPQQIHQLPGGTSTTTSGAGTQCIVVPQVVNQNGTHVQQPSNLQPQQQSVHPNMTQQPQQTPLNGHPSMVNTLQQQPPQQSLPMQTIQSQQQQHNQMPIISQQQQQQILMQQQQQQGSQQGSQQFNLPGTQQTHPQHQFIQSQPNQLHSLPPQVVSMAQGNLPHQLPPMQTMGAQQQMISQQQHQLQMQSHMHQQNVPGMYNQQTGARVAAPQNFAGAVPNHLLQQSPLMASQQPAQPMQHVMQPIFNATSGEVTEEPVSLAATHPHLLPSDIQSDIKHNLDSLVNQLCNTRLGTNQHQRLLLLRQRQLIEEDELRLKHYVEYEKFQKALRQSISTNVPANAAYYSAAAAQLPTNLAAPAAPSSSNPTSTSSANT

Target ORF information:

RefSeq Version	NM_001275243.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Wnk kinase (Wnk), transcript variant N, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275243.3

ORF Insert Sequence:

ATTGGACAAA CCCTCTGCGA CTTCGTACGA CAACAAGAGG TCGTACCTGC GAACCGTGCT 
CGGAAGGGAA GCGCCATCAG CGAAGATGGT ACTACCTCCT GCACCACGCA ACCTCAAAGG 
AATACCTCAA TTTCTAGCGA GGAGCAAACT GTGCAGGGAG CTAATATACA AAAGTCGGGA 
CACCGTTCGT TTAATAGATC TGCCTCCTCT AGGCAAAAGC CGAACCCGAC ATCAAAACTA 
AAAGACACAC TAAACAGAAA GCCCACTTGC AAGCAGGGCA CACTTTCTGC ACACACGAGC 
ACTAGCAGCA CCACTTCAAT CCAGTCATCA CCTATCGAGC CAGCTTCTTC TCTACCCACG 
CTGAACACCA CGCCTACACC ACCAGCTGCC AGCAAAAGTA ACCTATTTGT GCGGGTGCTA 
AGACGCTTTT CAAATAACAC CCTTCCCGGC AAAACGGCGT CAAGCCCGAA AAACGTGGAC 
GACGTACCGA AAGTCAACGA TAACAACAAC GGCGATTTAG GCAAACAGCA ATCAACCGAT 
GTAGAGGTGC TTTGCTCTCA GGACAACCAG AGCAGGGAAC AGAATGAGGA CGAAATGGAT 
AGCAAAATAG AACCTGCAGA TGCCATAAGC AACCAGAAAG CTGGTCTTAC TTCGGGCAAA 
CCTAAAAAAG ACAAGTCAGT CAAGGGGACG TCGCAAGCGG TTGTTTCAGA CACCAAGAAA 
ATGGAGGCCA GAAAGTCCTC CTCGGACACG GTGATCGAGC CATTGATCAA GCAGCAGGCG 
CCACTGCACT CGTCAAAGAC CACGCCAACG ACTTTGACGG CGAACTTCGT GCAAAACATT 
CGATTCGTCC GTAAAAATGT GGAACAGTCA GGCAGGAACA CCAATCCACT GCAGTTCGTT 
GAACTCGAGA CAGAGTTTCC TCGTGACTAT GACGACAACA TCGAGATGCT TTCCCGCGAG 
GCGGAGCATC TGGAGGAGCA GTTCCGCACG CCTACGCGCT CCAATGCTAC GGATGCCACT 
TCCCACCATG TGGCCGGAAT CATCGACAAC ATTATAACCG AAGCGTCACG GAGTCTTACA 
ATCGAAAAGA CTGAAGACGA TGTGCCATTG AAGTCTTCTA CCAAGCACTC AAGCGGCGTA 
AAACGAGTTG GCTTTCAAGT AGAGGATAAG GACGATACCG AAGTTCAGAG CGAAAAGCAG 
GCAAAGAGCT TCGACGACAT AACCAAAAAA GCGGAGAGCT CCGAGGCTAG CGCAGAGGAA 
GCTGCTGTCA CCGGGTCCTC GACAGATGCA TCTGCATCTC CCCTGCCCTC CACGTCGCTG 
GTCTCCACCA CATCTTCAGC TACATCGATA ACCAAGAGTA AGAGCGATGA GGACGATGAT 
CCGGTGGCCA TGTCGCCGTG CGGACGATTC TTTAAGTACG ATAAGGAAGT CGGACGCGGT 
TCCTTTAAAA CTGTCTATCG TGGCCTGGAC ACGTTGACTG GTGTTCCAGT TGCCTGGTGC 
GAGTTATTGG ACAAGCAAGT AAAAAAAAGT GAACGTACTA GATTTCGCGA GGAGGCAGAC 
ATGCTGAAGA AACTTCAGCA CCCAAACATA GTGCGGTTTT ATACATATTG GGAGTTTCCT 
ATTGGCCGCA AGAAGAATAT TGTACTAGTC ACCGAACTAA TGTTATCTGG AACTTTGAAA 
TCCTATTTGA AACGATTCAA AAAAATACAC CCAAAGGTAT TGAAGTCATG GTGTCGCCAG 
ATACTAAAAG GCCTTAACTT CCTGCATACT CGTCAATTTC CTATAATTCA TCGTGATTTG 
AAATGTGATA ATATTTTTAT AACTGGCACC ACTGGTAGTG TTAAAATCGG CGACTTGGGC 
CTGGCAACTT TGAAAAACCG CTCCCACGCG AAATCTGTGA TTGGTACGCC GGAGTTTATG 
GCCCCGGAAA TGTACGAGGA GCACTACGAC GAGTCGGTGG ACGTATATGC ATTCGGCATG 
TGCATGTTGG AGATGGCCAT TTCCGAATAT CCTTACAGTG AGTGCAAGGG TCCTGCACAG 
ATCTACAAAA AGGTGATATC GGGCATTAAG CCAGCGGCGT TGGCTAAGGT GGAGGATCCC 
AACGTGCGCG ACATTATCGA ACGGTGCATT GAACTTAAAA AGGAGGATCG CCCCAGCTGC 
AATGAACTGC TAGAATCGGA GTTCTTTGAC GAAGATATCG GTATACGAGT GGAGCCAACT 
GCTTCGGAGC AGTTCCTTTC TGATCCGAGC ATCAGCATCA TCGAGTTCCG ACTGCGGTTT 
ATGGATCCCA AGAAGCGCTC GTCCCGTCAT AAGGAAAACG AGGCAATTCA GTTTGAGTAC 
AACATCAGGC ATGACGAGTA CGAACAGATT GCGCAAGAAA TGATGAAGGA AAATATTATA 
TCTGAGGACG ACTCTCGCGC TGTAGCTCGG CTTTTAAAAG TGCAGGTGGT GTCGTTGCTT 
AAGGAACGTG CCCAGCGTCA AACTCAAATC AAGCTGCAAA ACGAGAAGTC TCGTTTGGAA 
AAATTGGCAT TGCAAAAGCA ACGTGAGAGT TTGCCGACTA ACGTCGATGA AGACGAGGAA 
GAGGAGGAGG AGTCCGAGGA CGAGGAGGAT GGTGTTAAAT GGAATCAGCG CCTGCAGCTA 
AGATATGACC TGCTTAACAC TGATTCAGAA ACAAGCCTGG CTCTATCCAC GAACAGCGTC 
GAACCGCAGC AGCTGTCCAC TCGCTCGAAC ACTTCGATTC CGAACAGTGG CATTCAGCAA 
CCTGTTCAGG TCCCAGGTCA AGTGCCTGTT TCCCAGTTGA TCTCGGTGCA ACCACAGGCT 
ATTCCGTCAC CGGCTATTCC CATGCAACAG AAGCCGACGG TGCACTACAT TCAACCGCCA 
CAGCTGGCCT CTTATCAAAA TTCGAATGCA TCGATGCAGG AGATGACCAA TAACCAGGTC 
ATTTCGCCGA CAGGCAGTCA ACAAATGCAA CAGCAACAAC CCGTAGTGGC ACCAACAGTC 
AATCATCAGG TCATGCCGCA GCAGCAAGTG AACCAACAAC AGCAACAACC ACAAATGATG 
CAGCAAATAC CCCAGCAAGT TCAAGTGCAA CAGCCCCAAA CTGTATTGCC TCCGCAGCCA 
CATGAGCAGC AGCCACAGCA ACAACAACAG CCACTGCAGC AACAACTGCA GCAGCTTATG 
CATACTAACG TGCAGGCTCC GGATTTGACT CAACAGCAAC AAATGGCTCA GCAACAGGCC 
CAGCAATATT TTCAGCAGCA GCAGCAACAG CCTCAGCAGG CCGTTAACAT GCAACAAGCT 
TATGCAATGC AGCAAGCTGG CCAACAGCAA CAGCTTTCGC AGCCTCTTCA AATTCAGCAA 
CAGATCTTGC AGCAACAACA AGTGGCCGTT TCACATCAGC AACAGATAAT GCAGCAACAA 
CTGGCCCAGC ATCAGCTGCA ACAGCTGCAG CAGCAGCAGC TTCAACAACA GCAACTGCAA 
CAGCAGCAGC AAATACAACA ACAGCAACTC CAACAACAAC AACTCCAGCA ACAGCAATTC 
GTACAGCAGT ATGCGCAGGC AATGCCTCAG CAACAACATC AGCAACTCGT TACGGGATCG 
CAAGTCATGG CACCGCATCA ACATCAGCAA CCTATTCAGA TCCCAGTGCA GATGCAAGTT 
CCACCGACGT CCGTGGCTCC TCCAATACAA CACACTTACA ATCAGCAGGG CGGACAAGTA 
ACGCTCAGTG ATGCCCAACA GCAACAGCAT CCTGGATTCT CGGCCGTACC ACAACAGGCA 
GCGCCATTTA TACAACAGCC CACACAGCAA CCCATTCAGC TATCAATGCC TTTGGAACAA 
CAATTGCAGC AATTGCTGCA TAGCCAGCCA GCCCAGCAAC AACAGGCCAT GAGCCAACAG 
CAGCAACAGC CCTTGGTGCA ACAGCAACAA CTGCCTTTGG TACAACAGCA GCCGCCTTTG 
GTCCAGCAGC AGCAACCGCT GGTTCAGCAT CAGCAGCCTT CGGTCCAACA TCAGCAACCT 
TTGGTGCAAC AGCCCCAGCA ACAGCAACCA CAGCCCCAGA ATCAGCAACC ACAGCCCCAG 
ACACAGCAAA CACACGTGGT TCAGCAACAA CCACCACAGC AGCAACCTGC AGTAGAGCAA 
ATCTCACAGA TTTCTTCGCA AGTGCCTGTT CAACAGGAAA ATCTGCAGCC AATACAGGTC 
AACAAAGATG CGAATGTCGC AACGGATGCA ATGTCCCTAA ATAGCGCCCA CGGTGCCCTC 
GAACCTGCAC CGAAGACCGA ACCCCAGAAC TCTGCTGATG CGGAAAAGCA GCAAAAGCAG 
ACAGGCACGG GCACACGTTC CCAGAAACCG AGGCGATCAA ACCGGAGCGG CAACGAACGG 
ATTCCCAAAC TTAGTGTAAC CAGCGTCGAC GAGGGCAGCG TCATAAACTG TCACATGGAA 
AATAAACTTA AAACGATTAC ATTTAAATTC GACATAGGAG ACGTAAATCC GGTTGAAATA 
GCCAATAAAT TGATCGCCCA AGATTTGCTT TCTAATTGTC AAAGTACAGT TTTTGTGGAA 
ATGATCAATG AAATTGTTGA CCAAGTCAAA CAGAATCCCA ATCAAATCCC TATACCGACA 
AATTATCGCC GCAATATTGA AAAGCGGGAT GAAACCGCCA GCGACATCAC AAAGATGTTC 
GAGCCCACTA TCCATGGTGT TGAAACCCTG CCATCCGGTG GAGGAGGCGC TGAACAGTCA 
AACTGCAACT TGACTTTAGA GGCCAGGTCC CATTTATCCA ACATACCAAA TGCGAAAGAA 
CCACAGCTGA ACGTCAGCAC TCCGCCCACA ACCACTTCGA CTATGTCTTC CTCATCAACC 
GCATCGAGGG ATGCGCCCAA TAGTAGCAAT GACGTGACCA TCGGCTCTGG TTCCGTTTCT 
CGAAAAACGA GCACGGCCTC GGAGTACACT TCGCTATCGA TTGACTATAT GCCCGACAGC 
AACATTACTC CAACTGGTCC GGAGCCACCG CTTGACGATG GCAAGGATAA AAAACCTTGC 
TCCCTTGCTA TAGCCAGAAT GCAAAAGCTC TTGGAGTCGG CCGGCAATGG TGCTCCTCGC 
AGCCTGAATT TGCCCTTGAA TCGTCACCTG AAAATTCAAG AGGACTTGAA GCATACTAGA 
AGTCTTGACG ATTTGACCGC AGTGAAAATA ACCTTTGACA TGCCCAACAA GGCTGCATTG 
GACACTTCGG AAAACGCTCA GCAGGCCACA GAAGCGGAAG CGGAGAAACC AAAAGATAAA 
TCCGGACAGG CAGTAGGCAA CCAGGGAACT GGAGCGGCCA ACACTCTAGA GCAGTTAAAA 
ATAGAACTGG AGAACATTAC TCATGCCCAC GCGTTTGCCT CCGCTGTAGT GGCCTCGATT 
AACAACAGAG CTCCTCATCA AGCTTCCCCA GCAATGTCCT CCCTAAAGGC CACTTCTGGA 
CAACCACAGA CACAAGAGAT AACCAAGCCA AATAACGGAG CAGGCCCTTC AGTACCCTCA 
GTGGGTCAGA ATACTCCCAC TGCAGCCTTA ACATCCGCCA GGGGCTCAGG CTCCTCCGTC 
TACAACTCAC GTCGCACATC CATCGACAAC AGCGTGGGAT CCGACATGCA CCTGCACACT 
GCCACTAATC TTGAAGGGAC AGTGAGCAAC CCGGATCCGA TCCCGACTGA GGCATCGGTT 
GGAATTACCA TTGCTACGGG TCATGAGAAG CAACTCTCCA AGCAGCCCAG TTTGGAAAAG 
CCTTCTGCCA CTTCCATTTT AACGAACAAC AGTGATCCTC CGCAAAGGAA TCCCAGCAAT 
GGATCAATTA ACCAAAATTC AATAGCTGAT TTGGAAAAGA AATTGGCAGC TTTGCGAAAT 
ACCGAAAACA CAGAAGAGTC TGCAACAGCA ACGTTGTCAG TGGTTCCTAA ACAAGTTGAA 
GAGGTAGTCA ATCCAAGCGC GCGTAAAATT TCGCGTTTTA GTGTCAGTCG TGTGCAGGAG 
CAGAAAACTT CAACCGGAGT TGAGGAACCA GCTCAAGGTC AACTTAAGAT CGACCTCCAA 
GTCGCCGGCC CTGGAGGCCA GGTGCAGACC AACAACTCCG TTCAGAACGG CAGCGTGGTC 
AACACTCCAA CCGAAGTCAT CAGCTCTCCT ATTCAAAATG TTCCGCTGGC CATTAACGGA 
ATTCAGTTGA TGTACCAACA GCCCCAGCAG ATACACCAGT TGCCAGGTGG AACATCAACT 
ACTACAAGTG GAGCAGGGAC TCAGTGCATT GTAGTGCCGC AAGTGGTCAA TCAAAATGGC 
ACCCACGTGC AACAGCCGTC TAACCTACAG CCACAGCAGC AGAGCGTACA TCCAAATATG 
ACTCAACAGC CACAGCAGAC GCCACTTAAT GGGCACCCAT CTATGGTTAA CACTCTTCAG 
CAGCAGCCAC CACAACAATC CTTACCGATG CAGACGATTC AGTCGCAGCA GCAGCAGCAT 
AACCAAATGC CTATCATTTC GCAACAACAG CAGCAGCAGA TCCTAATGCA GCAGCAACAG 
CAACAGGGAT CCCAGCAAGG ATCACAGCAA TTTAATTTGC CTGGCACACA ACAGACTCAT 
CCTCAGCACC AGTTCATCCA GTCGCAGCCC AACCAACTTC ACTCGCTTCC GCCCCAGGTA 
GTGAGCATGG CTCAGGGAAA TCTGCCGCAT CAGCTTCCAC CCATGCAGAC TATGGGAGCC 
CAACAGCAAA TGATCTCGCA ACAACAGCAT CAGTTGCAGA TGCAGTCGCA TATGCACCAA 
CAAAACGTGC CCGGAATGTA CAACCAGCAG ACTGGAGCTC GTGTGGCAGC ACCCCAAAAT 
TTCGCAGGTG CAGTTCCAAA TCATTTGTTG CAGCAGTCCC CTTTGATGGC ATCCCAGCAG 
CCAGCACAGC CCATGCAACA CGTTATGCAA CCGATTTTCA ATGCAACGTC CGGTGAAGTT 
ACCGAAGAGC CTGTCTCTTT AGCAGCTACG CATCCGCATC TGCTACCGAG CGATATTCAA 
TCCGATATAA AGCACAATTT AGACTCATTA GTGAACCAAT TGTGCAATAC GCGCTTGGGT 
ACTAACCAGC ACCAACGGCT GTTGCTGTTG CGTCAGAGAC AACTCATCGA AGAGGACGAG 
CTGCGTCTTA AACATTATGT GGAATACGAA AAGTTCCAAA AGGCACTGCG CCAATCTATT 
AGCACAAACG TTCCAGCTAA TGCAGCTTAC TATTCTGCGG CTGCCGCACA GCTTCCAACT 
AATTTGGCAG CCCCAGCAGC TCCGTCCTCA TCGAATCCGA CGTCCACTAG CTCCGCAAAC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wnk ( NM_001275245.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262174.3 Drosophila melanogaster Wnk kinase (Wnk), transcript variant L, mRNA.

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CloneID	OFa41367
Clone ID Related Accession (Same CDS sequence)	NM_001275243.3 , NM_001275245.3
Accession Version	NM_001275245.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Wnk kinase, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Oct 19, 2015 this sequence version replaced NM_001275245.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATTGGACAAA CCCTCTGCGA CTTCGTACGA CAACAAGAGG TCGTACCTGC GAACCGTGCT 
CGGAAGGGAA GCGCCATCAG CGAAGATGGT ACTACCTCCT GCACCACGCA ACCTCAAAGG 
AATACCTCAA TTTCTAGCGA GGAGCAAACT GTGCAGGGAG CTAATATACA AAAGTCGGGA 
CACCGTTCGT TTAATAGATC TGCCTCCTCT AGGCAAAAGC CGAACCCGAC ATCAAAACTA 
AAAGACACAC TAAACAGAAA GCCCACTTGC AAGCAGGGCA CACTTTCTGC ACACACGAGC 
ACTAGCAGCA CCACTTCAAT CCAGTCATCA CCTATCGAGC CAGCTTCTTC TCTACCCACG 
CTGAACACCA CGCCTACACC ACCAGCTGCC AGCAAAAGTA ACCTATTTGT GCGGGTGCTA 
AGACGCTTTT CAAATAACAC CCTTCCCGGC AAAACGGCGT CAAGCCCGAA AAACGTGGAC 
GACGTACCGA AAGTCAACGA TAACAACAAC GGCGATTTAG GCAAACAGCA ATCAACCGAT 
GTAGAGGTGC TTTGCTCTCA GGACAACCAG AGCAGGGAAC AGAATGAGGA CGAAATGGAT 
AGCAAAATAG AACCTGCAGA TGCCATAAGC AACCAGAAAG CTGGTCTTAC TTCGGGCAAA 
CCTAAAAAAG ACAAGTCAGT CAAGGGGACG TCGCAAGCGG TTGTTTCAGA CACCAAGAAA 
ATGGAGGCCA GAAAGTCCTC CTCGGACACG GTGATCGAGC CATTGATCAA GCAGCAGGCG 
CCACTGCACT CGTCAAAGAC CACGCCAACG ACTTTGACGG CGAACTTCGT GCAAAACATT 
CGATTCGTCC GTAAAAATGT GGAACAGTCA GGCAGGAACA CCAATCCACT GCAGTTCGTT 
GAACTCGAGA CAGAGTTTCC TCGTGACTAT GACGACAACA TCGAGATGCT TTCCCGCGAG 
GCGGAGCATC TGGAGGAGCA GTTCCGCACG CCTACGCGCT CCAATGCTAC GGATGCCACT 
TCCCACCATG TGGCCGGAAT CATCGACAAC ATTATAACCG AAGCGTCACG GAGTCTTACA 
ATCGAAAAGA CTGAAGACGA TGTGCCATTG AAGTCTTCTA CCAAGCACTC AAGCGGCGTA 
AAACGAGTTG GCTTTCAAGT AGAGGATAAG GACGATACCG AAGTTCAGAG CGAAAAGCAG 
GCAAAGAGCT TCGACGACAT AACCAAAAAA GCGGAGAGCT CCGAGGCTAG CGCAGAGGAA 
GCTGCTGTCA CCGGGTCCTC GACAGATGCA TCTGCATCTC CCCTGCCCTC CACGTCGCTG 
GTCTCCACCA CATCTTCAGC TACATCGATA ACCAAGAGTA AGAGCGATGA GGACGATGAT 
CCGGTGGCCA TGTCGCCGTG CGGACGATTC TTTAAGTACG ATAAGGAAGT CGGACGCGGT 
TCCTTTAAAA CTGTCTATCG TGGCCTGGAC ACGTTGACTG GTGTTCCAGT TGCCTGGTGC 
GAGTTATTGG ACAAGCAAGT AAAAAAAAGT GAACGTACTA GATTTCGCGA GGAGGCAGAC 
ATGCTGAAGA AACTTCAGCA CCCAAACATA GTGCGGTTTT ATACATATTG GGAGTTTCCT 
ATTGGCCGCA AGAAGAATAT TGTACTAGTC ACCGAACTAA TGTTATCTGG AACTTTGAAA 
TCCTATTTGA AACGATTCAA AAAAATACAC CCAAAGGTAT TGAAGTCATG GTGTCGCCAG 
ATACTAAAAG GCCTTAACTT CCTGCATACT CGTCAATTTC CTATAATTCA TCGTGATTTG 
AAATGTGATA ATATTTTTAT AACTGGCACC ACTGGTAGTG TTAAAATCGG CGACTTGGGC 
CTGGCAACTT TGAAAAACCG CTCCCACGCG AAATCTGTGA TTGGTACGCC GGAGTTTATG 
GCCCCGGAAA TGTACGAGGA GCACTACGAC GAGTCGGTGG ACGTATATGC ATTCGGCATG 
TGCATGTTGG AGATGGCCAT TTCCGAATAT CCTTACAGTG AGTGCAAGGG TCCTGCACAG 
ATCTACAAAA AGGTGATATC GGGCATTAAG CCAGCGGCGT TGGCTAAGGT GGAGGATCCC 
AACGTGCGCG ACATTATCGA ACGGTGCATT GAACTTAAAA AGGAGGATCG CCCCAGCTGC 
AATGAACTGC TAGAATCGGA GTTCTTTGAC GAAGATATCG GTATACGAGT GGAGCCAACT 
GCTTCGGAGC AGTTCCTTTC TGATCCGAGC ATCAGCATCA TCGAGTTCCG ACTGCGGTTT 
ATGGATCCCA AGAAGCGCTC GTCCCGTCAT AAGGAAAACG AGGCAATTCA GTTTGAGTAC 
AACATCAGGC ATGACGAGTA CGAACAGATT GCGCAAGAAA TGATGAAGGA AAATATTATA 
TCTGAGGACG ACTCTCGCGC TGTAGCTCGG CTTTTAAAAG TGCAGGTGGT GTCGTTGCTT 
AAGGAACGTG CCCAGCGTCA AACTCAAATC AAGCTGCAAA ACGAGAAGTC TCGTTTGGAA 
AAATTGGCAT TGCAAAAGCA ACGTGAGAGT TTGCCGACTA ACGTCGATGA AGACGAGGAA 
GAGGAGGAGG AGTCCGAGGA CGAGGAGGAT GGTGTTAAAT GGAATCAGCG CCTGCAGCTA 
AGATATGACC TGCTTAACAC TGATTCAGAA ACAAGCCTGG CTCTATCCAC GAACAGCGTC 
GAACCGCAGC AGCTGTCCAC TCGCTCGAAC ACTTCGATTC CGAACAGTGG CATTCAGCAA 
CCTGTTCAGG TCCCAGGTCA AGTGCCTGTT TCCCAGTTGA TCTCGGTGCA ACCACAGGCT 
ATTCCGTCAC CGGCTATTCC CATGCAACAG AAGCCGACGG TGCACTACAT TCAACCGCCA 
CAGCTGGCCT CTTATCAAAA TTCGAATGCA TCGATGCAGG AGATGACCAA TAACCAGGTC 
ATTTCGCCGA CAGGCAGTCA ACAAATGCAA CAGCAACAAC CCGTAGTGGC ACCAACAGTC 
AATCATCAGG TCATGCCGCA GCAGCAAGTG AACCAACAAC AGCAACAACC ACAAATGATG 
CAGCAAATAC CCCAGCAAGT TCAAGTGCAA CAGCCCCAAA CTGTATTGCC TCCGCAGCCA 
CATGAGCAGC AGCCACAGCA ACAACAACAG CCACTGCAGC AACAACTGCA GCAGCTTATG 
CATACTAACG TGCAGGCTCC GGATTTGACT CAACAGCAAC AAATGGCTCA GCAACAGGCC 
CAGCAATATT TTCAGCAGCA GCAGCAACAG CCTCAGCAGG CCGTTAACAT GCAACAAGCT 
TATGCAATGC AGCAAGCTGG CCAACAGCAA CAGCTTTCGC AGCCTCTTCA AATTCAGCAA 
CAGATCTTGC AGCAACAACA AGTGGCCGTT TCACATCAGC AACAGATAAT GCAGCAACAA 
CTGGCCCAGC ATCAGCTGCA ACAGCTGCAG CAGCAGCAGC TTCAACAACA GCAACTGCAA 
CAGCAGCAGC AAATACAACA ACAGCAACTC CAACAACAAC AACTCCAGCA ACAGCAATTC 
GTACAGCAGT ATGCGCAGGC AATGCCTCAG CAACAACATC AGCAACTCGT TACGGGATCG 
CAAGTCATGG CACCGCATCA ACATCAGCAA CCTATTCAGA TCCCAGTGCA GATGCAAGTT 
CCACCGACGT CCGTGGCTCC TCCAATACAA CACACTTACA ATCAGCAGGG CGGACAAGTA 
ACGCTCAGTG ATGCCCAACA GCAACAGCAT CCTGGATTCT CGGCCGTACC ACAACAGGCA 
GCGCCATTTA TACAACAGCC CACACAGCAA CCCATTCAGC TATCAATGCC TTTGGAACAA 
CAATTGCAGC AATTGCTGCA TAGCCAGCCA GCCCAGCAAC AACAGGCCAT GAGCCAACAG 
CAGCAACAGC CCTTGGTGCA ACAGCAACAA CTGCCTTTGG TACAACAGCA GCCGCCTTTG 
GTCCAGCAGC AGCAACCGCT GGTTCAGCAT CAGCAGCCTT CGGTCCAACA TCAGCAACCT 
TTGGTGCAAC AGCCCCAGCA ACAGCAACCA CAGCCCCAGA ATCAGCAACC ACAGCCCCAG 
ACACAGCAAA CACACGTGGT TCAGCAACAA CCACCACAGC AGCAACCTGC AGTAGAGCAA 
ATCTCACAGA TTTCTTCGCA AGTGCCTGTT CAACAGGAAA ATCTGCAGCC AATACAGGTC 
AACAAAGATG CGAATGTCGC AACGGATGCA ATGTCCCTAA ATAGCGCCCA CGGTGCCCTC 
GAACCTGCAC CGAAGACCGA ACCCCAGAAC TCTGCTGATG CGGAAAAGCA GCAAAAGCAG 
ACAGGCACGG GCACACGTTC CCAGAAACCG AGGCGATCAA ACCGGAGCGG CAACGAACGG 
ATTCCCAAAC TTAGTGTAAC CAGCGTCGAC GAGGGCAGCG TCATAAACTG TCACATGGAA 
AATAAACTTA AAACGATTAC ATTTAAATTC GACATAGGAG ACGTAAATCC GGTTGAAATA 
GCCAATAAAT TGATCGCCCA AGATTTGCTT TCTAATTGTC AAAGTACAGT TTTTGTGGAA 
ATGATCAATG AAATTGTTGA CCAAGTCAAA CAGAATCCCA ATCAAATCCC TATACCGACA 
AATTATCGCC GCAATATTGA AAAGCGGGAT GAAACCGCCA GCGACATCAC AAAGATGTTC 
GAGCCCACTA TCCATGGTGT TGAAACCCTG CCATCCGGTG GAGGAGGCGC TGAACAGTCA 
AACTGCAACT TGACTTTAGA GGCCAGGTCC CATTTATCCA ACATACCAAA TGCGAAAGAA 
CCACAGCTGA ACGTCAGCAC TCCGCCCACA ACCACTTCGA CTATGTCTTC CTCATCAACC 
GCATCGAGGG ATGCGCCCAA TAGTAGCAAT GACGTGACCA TCGGCTCTGG TTCCGTTTCT 
CGAAAAACGA GCACGGCCTC GGAGTACACT TCGCTATCGA TTGACTATAT GCCCGACAGC 
AACATTACTC CAACTGGTCC GGAGCCACCG CTTGACGATG GCAAGGATAA AAAACCTTGC 
TCCCTTGCTA TAGCCAGAAT GCAAAAGCTC TTGGAGTCGG CCGGCAATGG TGCTCCTCGC 
AGCCTGAATT TGCCCTTGAA TCGTCACCTG AAAATTCAAG AGGACTTGAA GCATACTAGA 
AGTCTTGACG ATTTGACCGC AGTGAAAATA ACCTTTGACA TGCCCAACAA GGCTGCATTG 
GACACTTCGG AAAACGCTCA GCAGGCCACA GAAGCGGAAG CGGAGAAACC AAAAGATAAA 
TCCGGACAGG CAGTAGGCAA CCAGGGAACT GGAGCGGCCA ACACTCTAGA GCAGTTAAAA 
ATAGAACTGG AGAACATTAC TCATGCCCAC GCGTTTGCCT CCGCTGTAGT GGCCTCGATT 
AACAACAGAG CTCCTCATCA AGCTTCCCCA GCAATGTCCT CCCTAAAGGC CACTTCTGGA 
CAACCACAGA CACAAGAGAT AACCAAGCCA AATAACGGAG CAGGCCCTTC AGTACCCTCA 
GTGGGTCAGA ATACTCCCAC TGCAGCCTTA ACATCCGCCA GGGGCTCAGG CTCCTCCGTC 
TACAACTCAC GTCGCACATC CATCGACAAC AGCGTGGGAT CCGACATGCA CCTGCACACT 
GCCACTAATC TTGAAGGGAC AGTGAGCAAC CCGGATCCGA TCCCGACTGA GGCATCGGTT 
GGAATTACCA TTGCTACGGG TCATGAGAAG CAACTCTCCA AGCAGCCCAG TTTGGAAAAG 
CCTTCTGCCA CTTCCATTTT AACGAACAAC AGTGATCCTC CGCAAAGGAA TCCCAGCAAT 
GGATCAATTA ACCAAAATTC AATAGCTGAT TTGGAAAAGA AATTGGCAGC TTTGCGAAAT 
ACCGAAAACA CAGAAGAGTC TGCAACAGCA ACGTTGTCAG TGGTTCCTAA ACAAGTTGAA 
GAGGTAGTCA ATCCAAGCGC GCGTAAAATT TCGCGTTTTA GTGTCAGTCG TGTGCAGGAG 
CAGAAAACTT CAACCGGAGT TGAGGAACCA GCTCAAGGTC AACTTAAGAT CGACCTCCAA 
GTCGCCGGCC CTGGAGGCCA GGTGCAGACC AACAACTCCG TTCAGAACGG CAGCGTGGTC 
AACACTCCAA CCGAAGTCAT CAGCTCTCCT ATTCAAAATG TTCCGCTGGC CATTAACGGA 
ATTCAGTTGA TGTACCAACA GCCCCAGCAG ATACACCAGT TGCCAGGTGG AACATCAACT 
ACTACAAGTG GAGCAGGGAC TCAGTGCATT GTAGTGCCGC AAGTGGTCAA TCAAAATGGC 
ACCCACGTGC AACAGCCGTC TAACCTACAG CCACAGCAGC AGAGCGTACA TCCAAATATG 
ACTCAACAGC CACAGCAGAC GCCACTTAAT GGGCACCCAT CTATGGTTAA CACTCTTCAG 
CAGCAGCCAC CACAACAATC CTTACCGATG CAGACGATTC AGTCGCAGCA GCAGCAGCAT 
AACCAAATGC CTATCATTTC GCAACAACAG CAGCAGCAGA TCCTAATGCA GCAGCAACAG 
CAACAGGGAT CCCAGCAAGG ATCACAGCAA TTTAATTTGC CTGGCACACA ACAGACTCAT 
CCTCAGCACC AGTTCATCCA GTCGCAGCCC AACCAACTTC ACTCGCTTCC GCCCCAGGTA 
GTGAGCATGG CTCAGGGAAA TCTGCCGCAT CAGCTTCCAC CCATGCAGAC TATGGGAGCC 
CAACAGCAAA TGATCTCGCA ACAACAGCAT CAGTTGCAGA TGCAGTCGCA TATGCACCAA 
CAAAACGTGC CCGGAATGTA CAACCAGCAG ACTGGAGCTC GTGTGGCAGC ACCCCAAAAT 
TTCGCAGGTG CAGTTCCAAA TCATTTGTTG CAGCAGTCCC CTTTGATGGC ATCCCAGCAG 
CCAGCACAGC CCATGCAACA CGTTATGCAA CCGATTTTCA ATGCAACGTC CGGTGAAGTT 
ACCGAAGAGC CTGTCTCTTT AGCAGCTACG CATCCGCATC TGCTACCGAG CGATATTCAA 
TCCGATATAA AGCACAATTT AGACTCATTA GTGAACCAAT TGTGCAATAC GCGCTTGGGT 
ACTAACCAGC ACCAACGGCT GTTGCTGTTG CGTCAGAGAC AACTCATCGA AGAGGACGAG 
CTGCGTCTTA AACATTATGT GGAATACGAA AAGTTCCAAA AGGCACTGCG CCAATCTATT 
AGCACAAACG TTCCAGCTAA TGCAGCTTAC TATTCTGCGG CTGCCGCACA GCTTCCAACT 
AATTTGGCAG CCCCAGCAGC TCCGTCCTCA TCGAATCCGA CGTCCACTAG CTCCGCAAAC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262174.3
CDS	413..7738
Translation	IGQTLCDFVRQQEVVPANRARKGSAISEDGTTSCTTQPQRNTSISSEEQTVQGANIQKSGHRSFNRSASSRQKPNPTSKLKDTLNRKPTCKQGTLSAHTSTSSTTSIQSSPIEPASSLPTLNTTPTPPAASKSNLFVRVLRRFSNNTLPGKTASSPKNVDDVPKVNDNNNGDLGKQQSTDVEVLCSQDNQSREQNEDEMDSKIEPADAISNQKAGLTSGKPKKDKSVKGTSQAVVSDTKKMEARKSSSDTVIEPLIKQQAPLHSSKTTPTTLTANFVQNIRFVRKNVEQSGRNTNPLQFVELETEFPRDYDDNIEMLSREAEHLEEQFRTPTRSNATDATSHHVAGIIDNIITEASRSLTIEKTEDDVPLKSSTKHSSGVKRVGFQVEDKDDTEVQSEKQAKSFDDITKKAESSEASAEEAAVTGSSTDASASPLPSTSLVSTTSSATSITKSKSDEDDDPVAMSPCGRFFKYDKEVGRGSFKTVYRGLDTLTGVPVAWCELLDKQVKKSERTRFREEADMLKKLQHPNIVRFYTYWEFPIGRKKNIVLVTELMLSGTLKSYLKRFKKIHPKVLKSWCRQILKGLNFLHTRQFPIIHRDLKCDNIFITGTTGSVKIGDLGLATLKNRSHAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMAISEYPYSECKGPAQIYKKVISGIKPAALAKVEDPNVRDIIERCIELKKEDRPSCNELLESEFFDEDIGIRVEPTASEQFLSDPSISIIEFRLRFMDPKKRSSRHKENEAIQFEYNIRHDEYEQIAQEMMKENIISEDDSRAVARLLKVQVVSLLKERAQRQTQIKLQNEKSRLEKLALQKQRESLPTNVDEDEEEEEESEDEEDGVKWNQRLQLRYDLLNTDSETSLALSTNSVEPQQLSTRSNTSIPNSGIQQPVQVPGQVPVSQLISVQPQAIPSPAIPMQQKPTVHYIQPPQLASYQNSNASMQEMTNNQVISPTGSQQMQQQQPVVAPTVNHQVMPQQQVNQQQQQPQMMQQIPQQVQVQQPQTVLPPQPHEQQPQQQQQPLQQQLQQLMHTNVQAPDLTQQQQMAQQQAQQYFQQQQQQPQQAVNMQQAYAMQQAGQQQQLSQPLQIQQQILQQQQVAVSHQQQIMQQQLAQHQLQQLQQQQLQQQQLQQQQQIQQQQLQQQQLQQQQFVQQYAQAMPQQQHQQLVTGSQVMAPHQHQQPIQIPVQMQVPPTSVAPPIQHTYNQQGGQVTLSDAQQQQHPGFSAVPQQAAPFIQQPTQQPIQLSMPLEQQLQQLLHSQPAQQQQAMSQQQQQPLVQQQQLPLVQQQPPLVQQQQPLVQHQQPSVQHQQPLVQQPQQQQPQPQNQQPQPQTQQTHVVQQQPPQQQPAVEQISQISSQVPVQQENLQPIQVNKDANVATDAMSLNSAHGALEPAPKTEPQNSADAEKQQKQTGTGTRSQKPRRSNRSGNERIPKLSVTSVDEGSVINCHMENKLKTITFKFDIGDVNPVEIANKLIAQDLLSNCQSTVFVEMINEIVDQVKQNPNQIPIPTNYRRNIEKRDETASDITKMFEPTIHGVETLPSGGGGAEQSNCNLTLEARSHLSNIPNAKEPQLNVSTPPTTTSTMSSSSTASRDAPNSSNDVTIGSGSVSRKTSTASEYTSLSIDYMPDSNITPTGPEPPLDDGKDKKPCSLAIARMQKLLESAGNGAPRSLNLPLNRHLKIQEDLKHTRSLDDLTAVKITFDMPNKAALDTSENAQQATEAEAEKPKDKSGQAVGNQGTGAANTLEQLKIELENITHAHAFASAVVASINNRAPHQASPAMSSLKATSGQPQTQEITKPNNGAGPSVPSVGQNTPTAALTSARGSGSSVYNSRRTSIDNSVGSDMHLHTATNLEGTVSNPDPIPTEASVGITIATGHEKQLSKQPSLEKPSATSILTNNSDPPQRNPSNGSINQNSIADLEKKLAALRNTENTEESATATLSVVPKQVEEVVNPSARKISRFSVSRVQEQKTSTGVEEPAQGQLKIDLQVAGPGGQVQTNNSVQNGSVVNTPTEVISSPIQNVPLAINGIQLMYQQPQQIHQLPGGTSTTTSGAGTQCIVVPQVVNQNGTHVQQPSNLQPQQQSVHPNMTQQPQQTPLNGHPSMVNTLQQQPPQQSLPMQTIQSQQQQHNQMPIISQQQQQQILMQQQQQQGSQQGSQQFNLPGTQQTHPQHQFIQSQPNQLHSLPPQVVSMAQGNLPHQLPPMQTMGAQQQMISQQQHQLQMQSHMHQQNVPGMYNQQTGARVAAPQNFAGAVPNHLLQQSPLMASQQPAQPMQHVMQPIFNATSGEVTEEPVSLAATHPHLLPSDIQSDIKHNLDSLVNQLCNTRLGTNQHQRLLLLRQRQLIEEDELRLKHYVEYEKFQKALRQSISTNVPANAAYYSAAAAQLPTNLAAPAAPSSSNPTSTSSANT

Target ORF information:

RefSeq Version	NM_001275245.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Wnk kinase (Wnk), transcript variant L, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275245.3

ORF Insert Sequence:

ATTGGACAAA CCCTCTGCGA CTTCGTACGA CAACAAGAGG TCGTACCTGC GAACCGTGCT 
CGGAAGGGAA GCGCCATCAG CGAAGATGGT ACTACCTCCT GCACCACGCA ACCTCAAAGG 
AATACCTCAA TTTCTAGCGA GGAGCAAACT GTGCAGGGAG CTAATATACA AAAGTCGGGA 
CACCGTTCGT TTAATAGATC TGCCTCCTCT AGGCAAAAGC CGAACCCGAC ATCAAAACTA 
AAAGACACAC TAAACAGAAA GCCCACTTGC AAGCAGGGCA CACTTTCTGC ACACACGAGC 
ACTAGCAGCA CCACTTCAAT CCAGTCATCA CCTATCGAGC CAGCTTCTTC TCTACCCACG 
CTGAACACCA CGCCTACACC ACCAGCTGCC AGCAAAAGTA ACCTATTTGT GCGGGTGCTA 
AGACGCTTTT CAAATAACAC CCTTCCCGGC AAAACGGCGT CAAGCCCGAA AAACGTGGAC 
GACGTACCGA AAGTCAACGA TAACAACAAC GGCGATTTAG GCAAACAGCA ATCAACCGAT 
GTAGAGGTGC TTTGCTCTCA GGACAACCAG AGCAGGGAAC AGAATGAGGA CGAAATGGAT 
AGCAAAATAG AACCTGCAGA TGCCATAAGC AACCAGAAAG CTGGTCTTAC TTCGGGCAAA 
CCTAAAAAAG ACAAGTCAGT CAAGGGGACG TCGCAAGCGG TTGTTTCAGA CACCAAGAAA 
ATGGAGGCCA GAAAGTCCTC CTCGGACACG GTGATCGAGC CATTGATCAA GCAGCAGGCG 
CCACTGCACT CGTCAAAGAC CACGCCAACG ACTTTGACGG CGAACTTCGT GCAAAACATT 
CGATTCGTCC GTAAAAATGT GGAACAGTCA GGCAGGAACA CCAATCCACT GCAGTTCGTT 
GAACTCGAGA CAGAGTTTCC TCGTGACTAT GACGACAACA TCGAGATGCT TTCCCGCGAG 
GCGGAGCATC TGGAGGAGCA GTTCCGCACG CCTACGCGCT CCAATGCTAC GGATGCCACT 
TCCCACCATG TGGCCGGAAT CATCGACAAC ATTATAACCG AAGCGTCACG GAGTCTTACA 
ATCGAAAAGA CTGAAGACGA TGTGCCATTG AAGTCTTCTA CCAAGCACTC AAGCGGCGTA 
AAACGAGTTG GCTTTCAAGT AGAGGATAAG GACGATACCG AAGTTCAGAG CGAAAAGCAG 
GCAAAGAGCT TCGACGACAT AACCAAAAAA GCGGAGAGCT CCGAGGCTAG CGCAGAGGAA 
GCTGCTGTCA CCGGGTCCTC GACAGATGCA TCTGCATCTC CCCTGCCCTC CACGTCGCTG 
GTCTCCACCA CATCTTCAGC TACATCGATA ACCAAGAGTA AGAGCGATGA GGACGATGAT 
CCGGTGGCCA TGTCGCCGTG CGGACGATTC TTTAAGTACG ATAAGGAAGT CGGACGCGGT 
TCCTTTAAAA CTGTCTATCG TGGCCTGGAC ACGTTGACTG GTGTTCCAGT TGCCTGGTGC 
GAGTTATTGG ACAAGCAAGT AAAAAAAAGT GAACGTACTA GATTTCGCGA GGAGGCAGAC 
ATGCTGAAGA AACTTCAGCA CCCAAACATA GTGCGGTTTT ATACATATTG GGAGTTTCCT 
ATTGGCCGCA AGAAGAATAT TGTACTAGTC ACCGAACTAA TGTTATCTGG AACTTTGAAA 
TCCTATTTGA AACGATTCAA AAAAATACAC CCAAAGGTAT TGAAGTCATG GTGTCGCCAG 
ATACTAAAAG GCCTTAACTT CCTGCATACT CGTCAATTTC CTATAATTCA TCGTGATTTG 
AAATGTGATA ATATTTTTAT AACTGGCACC ACTGGTAGTG TTAAAATCGG CGACTTGGGC 
CTGGCAACTT TGAAAAACCG CTCCCACGCG AAATCTGTGA TTGGTACGCC GGAGTTTATG 
GCCCCGGAAA TGTACGAGGA GCACTACGAC GAGTCGGTGG ACGTATATGC ATTCGGCATG 
TGCATGTTGG AGATGGCCAT TTCCGAATAT CCTTACAGTG AGTGCAAGGG TCCTGCACAG 
ATCTACAAAA AGGTGATATC GGGCATTAAG CCAGCGGCGT TGGCTAAGGT GGAGGATCCC 
AACGTGCGCG ACATTATCGA ACGGTGCATT GAACTTAAAA AGGAGGATCG CCCCAGCTGC 
AATGAACTGC TAGAATCGGA GTTCTTTGAC GAAGATATCG GTATACGAGT GGAGCCAACT 
GCTTCGGAGC AGTTCCTTTC TGATCCGAGC ATCAGCATCA TCGAGTTCCG ACTGCGGTTT 
ATGGATCCCA AGAAGCGCTC GTCCCGTCAT AAGGAAAACG AGGCAATTCA GTTTGAGTAC 
AACATCAGGC ATGACGAGTA CGAACAGATT GCGCAAGAAA TGATGAAGGA AAATATTATA 
TCTGAGGACG ACTCTCGCGC TGTAGCTCGG CTTTTAAAAG TGCAGGTGGT GTCGTTGCTT 
AAGGAACGTG CCCAGCGTCA AACTCAAATC AAGCTGCAAA ACGAGAAGTC TCGTTTGGAA 
AAATTGGCAT TGCAAAAGCA ACGTGAGAGT TTGCCGACTA ACGTCGATGA AGACGAGGAA 
GAGGAGGAGG AGTCCGAGGA CGAGGAGGAT GGTGTTAAAT GGAATCAGCG CCTGCAGCTA 
AGATATGACC TGCTTAACAC TGATTCAGAA ACAAGCCTGG CTCTATCCAC GAACAGCGTC 
GAACCGCAGC AGCTGTCCAC TCGCTCGAAC ACTTCGATTC CGAACAGTGG CATTCAGCAA 
CCTGTTCAGG TCCCAGGTCA AGTGCCTGTT TCCCAGTTGA TCTCGGTGCA ACCACAGGCT 
ATTCCGTCAC CGGCTATTCC CATGCAACAG AAGCCGACGG TGCACTACAT TCAACCGCCA 
CAGCTGGCCT CTTATCAAAA TTCGAATGCA TCGATGCAGG AGATGACCAA TAACCAGGTC 
ATTTCGCCGA CAGGCAGTCA ACAAATGCAA CAGCAACAAC CCGTAGTGGC ACCAACAGTC 
AATCATCAGG TCATGCCGCA GCAGCAAGTG AACCAACAAC AGCAACAACC ACAAATGATG 
CAGCAAATAC CCCAGCAAGT TCAAGTGCAA CAGCCCCAAA CTGTATTGCC TCCGCAGCCA 
CATGAGCAGC AGCCACAGCA ACAACAACAG CCACTGCAGC AACAACTGCA GCAGCTTATG 
CATACTAACG TGCAGGCTCC GGATTTGACT CAACAGCAAC AAATGGCTCA GCAACAGGCC 
CAGCAATATT TTCAGCAGCA GCAGCAACAG CCTCAGCAGG CCGTTAACAT GCAACAAGCT 
TATGCAATGC AGCAAGCTGG CCAACAGCAA CAGCTTTCGC AGCCTCTTCA AATTCAGCAA 
CAGATCTTGC AGCAACAACA AGTGGCCGTT TCACATCAGC AACAGATAAT GCAGCAACAA 
CTGGCCCAGC ATCAGCTGCA ACAGCTGCAG CAGCAGCAGC TTCAACAACA GCAACTGCAA 
CAGCAGCAGC AAATACAACA ACAGCAACTC CAACAACAAC AACTCCAGCA ACAGCAATTC 
GTACAGCAGT ATGCGCAGGC AATGCCTCAG CAACAACATC AGCAACTCGT TACGGGATCG 
CAAGTCATGG CACCGCATCA ACATCAGCAA CCTATTCAGA TCCCAGTGCA GATGCAAGTT 
CCACCGACGT CCGTGGCTCC TCCAATACAA CACACTTACA ATCAGCAGGG CGGACAAGTA 
ACGCTCAGTG ATGCCCAACA GCAACAGCAT CCTGGATTCT CGGCCGTACC ACAACAGGCA 
GCGCCATTTA TACAACAGCC CACACAGCAA CCCATTCAGC TATCAATGCC TTTGGAACAA 
CAATTGCAGC AATTGCTGCA TAGCCAGCCA GCCCAGCAAC AACAGGCCAT GAGCCAACAG 
CAGCAACAGC CCTTGGTGCA ACAGCAACAA CTGCCTTTGG TACAACAGCA GCCGCCTTTG 
GTCCAGCAGC AGCAACCGCT GGTTCAGCAT CAGCAGCCTT CGGTCCAACA TCAGCAACCT 
TTGGTGCAAC AGCCCCAGCA ACAGCAACCA CAGCCCCAGA ATCAGCAACC ACAGCCCCAG 
ACACAGCAAA CACACGTGGT TCAGCAACAA CCACCACAGC AGCAACCTGC AGTAGAGCAA 
ATCTCACAGA TTTCTTCGCA AGTGCCTGTT CAACAGGAAA ATCTGCAGCC AATACAGGTC 
AACAAAGATG CGAATGTCGC AACGGATGCA ATGTCCCTAA ATAGCGCCCA CGGTGCCCTC 
GAACCTGCAC CGAAGACCGA ACCCCAGAAC TCTGCTGATG CGGAAAAGCA GCAAAAGCAG 
ACAGGCACGG GCACACGTTC CCAGAAACCG AGGCGATCAA ACCGGAGCGG CAACGAACGG 
ATTCCCAAAC TTAGTGTAAC CAGCGTCGAC GAGGGCAGCG TCATAAACTG TCACATGGAA 
AATAAACTTA AAACGATTAC ATTTAAATTC GACATAGGAG ACGTAAATCC GGTTGAAATA 
GCCAATAAAT TGATCGCCCA AGATTTGCTT TCTAATTGTC AAAGTACAGT TTTTGTGGAA 
ATGATCAATG AAATTGTTGA CCAAGTCAAA CAGAATCCCA ATCAAATCCC TATACCGACA 
AATTATCGCC GCAATATTGA AAAGCGGGAT GAAACCGCCA GCGACATCAC AAAGATGTTC 
GAGCCCACTA TCCATGGTGT TGAAACCCTG CCATCCGGTG GAGGAGGCGC TGAACAGTCA 
AACTGCAACT TGACTTTAGA GGCCAGGTCC CATTTATCCA ACATACCAAA TGCGAAAGAA 
CCACAGCTGA ACGTCAGCAC TCCGCCCACA ACCACTTCGA CTATGTCTTC CTCATCAACC 
GCATCGAGGG ATGCGCCCAA TAGTAGCAAT GACGTGACCA TCGGCTCTGG TTCCGTTTCT 
CGAAAAACGA GCACGGCCTC GGAGTACACT TCGCTATCGA TTGACTATAT GCCCGACAGC 
AACATTACTC CAACTGGTCC GGAGCCACCG CTTGACGATG GCAAGGATAA AAAACCTTGC 
TCCCTTGCTA TAGCCAGAAT GCAAAAGCTC TTGGAGTCGG CCGGCAATGG TGCTCCTCGC 
AGCCTGAATT TGCCCTTGAA TCGTCACCTG AAAATTCAAG AGGACTTGAA GCATACTAGA 
AGTCTTGACG ATTTGACCGC AGTGAAAATA ACCTTTGACA TGCCCAACAA GGCTGCATTG 
GACACTTCGG AAAACGCTCA GCAGGCCACA GAAGCGGAAG CGGAGAAACC AAAAGATAAA 
TCCGGACAGG CAGTAGGCAA CCAGGGAACT GGAGCGGCCA ACACTCTAGA GCAGTTAAAA 
ATAGAACTGG AGAACATTAC TCATGCCCAC GCGTTTGCCT CCGCTGTAGT GGCCTCGATT 
AACAACAGAG CTCCTCATCA AGCTTCCCCA GCAATGTCCT CCCTAAAGGC CACTTCTGGA 
CAACCACAGA CACAAGAGAT AACCAAGCCA AATAACGGAG CAGGCCCTTC AGTACCCTCA 
GTGGGTCAGA ATACTCCCAC TGCAGCCTTA ACATCCGCCA GGGGCTCAGG CTCCTCCGTC 
TACAACTCAC GTCGCACATC CATCGACAAC AGCGTGGGAT CCGACATGCA CCTGCACACT 
GCCACTAATC TTGAAGGGAC AGTGAGCAAC CCGGATCCGA TCCCGACTGA GGCATCGGTT 
GGAATTACCA TTGCTACGGG TCATGAGAAG CAACTCTCCA AGCAGCCCAG TTTGGAAAAG 
CCTTCTGCCA CTTCCATTTT AACGAACAAC AGTGATCCTC CGCAAAGGAA TCCCAGCAAT 
GGATCAATTA ACCAAAATTC AATAGCTGAT TTGGAAAAGA AATTGGCAGC TTTGCGAAAT 
ACCGAAAACA CAGAAGAGTC TGCAACAGCA ACGTTGTCAG TGGTTCCTAA ACAAGTTGAA 
GAGGTAGTCA ATCCAAGCGC GCGTAAAATT TCGCGTTTTA GTGTCAGTCG TGTGCAGGAG 
CAGAAAACTT CAACCGGAGT TGAGGAACCA GCTCAAGGTC AACTTAAGAT CGACCTCCAA 
GTCGCCGGCC CTGGAGGCCA GGTGCAGACC AACAACTCCG TTCAGAACGG CAGCGTGGTC 
AACACTCCAA CCGAAGTCAT CAGCTCTCCT ATTCAAAATG TTCCGCTGGC CATTAACGGA 
ATTCAGTTGA TGTACCAACA GCCCCAGCAG ATACACCAGT TGCCAGGTGG AACATCAACT 
ACTACAAGTG GAGCAGGGAC TCAGTGCATT GTAGTGCCGC AAGTGGTCAA TCAAAATGGC 
ACCCACGTGC AACAGCCGTC TAACCTACAG CCACAGCAGC AGAGCGTACA TCCAAATATG 
ACTCAACAGC CACAGCAGAC GCCACTTAAT GGGCACCCAT CTATGGTTAA CACTCTTCAG 
CAGCAGCCAC CACAACAATC CTTACCGATG CAGACGATTC AGTCGCAGCA GCAGCAGCAT 
AACCAAATGC CTATCATTTC GCAACAACAG CAGCAGCAGA TCCTAATGCA GCAGCAACAG 
CAACAGGGAT CCCAGCAAGG ATCACAGCAA TTTAATTTGC CTGGCACACA ACAGACTCAT 
CCTCAGCACC AGTTCATCCA GTCGCAGCCC AACCAACTTC ACTCGCTTCC GCCCCAGGTA 
GTGAGCATGG CTCAGGGAAA TCTGCCGCAT CAGCTTCCAC CCATGCAGAC TATGGGAGCC 
CAACAGCAAA TGATCTCGCA ACAACAGCAT CAGTTGCAGA TGCAGTCGCA TATGCACCAA 
CAAAACGTGC CCGGAATGTA CAACCAGCAG ACTGGAGCTC GTGTGGCAGC ACCCCAAAAT 
TTCGCAGGTG CAGTTCCAAA TCATTTGTTG CAGCAGTCCC CTTTGATGGC ATCCCAGCAG 
CCAGCACAGC CCATGCAACA CGTTATGCAA CCGATTTTCA ATGCAACGTC CGGTGAAGTT 
ACCGAAGAGC CTGTCTCTTT AGCAGCTACG CATCCGCATC TGCTACCGAG CGATATTCAA 
TCCGATATAA AGCACAATTT AGACTCATTA GTGAACCAAT TGTGCAATAC GCGCTTGGGT 
ACTAACCAGC ACCAACGGCT GTTGCTGTTG CGTCAGAGAC AACTCATCGA AGAGGACGAG 
CTGCGTCTTA AACATTATGT GGAATACGAA AAGTTCCAAA AGGCACTGCG CCAATCTATT 
AGCACAAACG TTCCAGCTAA TGCAGCTTAC TATTCTGCGG CTGCCGCACA GCTTCCAACT 
AATTTGGCAG CCCCAGCAGC TCCGTCCTCA TCGAATCCGA CGTCCACTAG CTCCGCAAAC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wnk ( NM_001275242.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262171.2 Drosophila melanogaster Wnk kinase (Wnk), transcript variant M, mRNA.

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CloneID	OFa41368
Clone ID Related Accession (Same CDS sequence)	NM_001275242.2
Accession Version	NM_001275242.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Wnk kinase, isoform M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Oct 19, 2015 this sequence version replaced NM_001275242.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATTGGACAAA CCCTCTGCGA CTTCGTACGA CAACAAGAGG TCGTACCTGC GAACCGTGCT 
CGGAAGGGAA GCGCCATCAG CGAAGATGGT ACTACCTCCT GCACCACGCA ACCTCAAAGG 
AATACCTCAA TTTCTAGCGA GGAGCAAACT GTGCAGGGAG CTAATATACA AAAGTCGGGA 
CACCGTTCGT TTAATAGATC TGCCTCCTCT AGGCAAAAGC CGAACCCGAC ATCAAAACTA 
AAAGACACAC TAAACAGAAA GCCCACTTGC AAGCAGGGCA CACTTTCTGC ACACACGAGC 
ACTAGCAGCA CCACTTCAAT CCAGTCATCA CCTATCGAGC CAGCTTCTTC TCTACCCACG 
CTGAACACCA CGCCTACACC ACCAGCTGCC AGCAAAAGTA ACCTATTTGT GCGGGTGCTA 
AGACGCTTTT CAAATAACAC CCTTCCCGGC AAAACGGCGT CAAGCCCGAA AAACGTGGAC 
GACGTACCGA AAGTCAACGA TAACAACAAC GGCGATTTAG GCAAACAGCA ATCAACCGAT 
GTAGAGGTGC TTTGCTCTCA GGACAACCAG AGCAGGGAAC AGAATGAGGA CGAAATGGAT 
AGCAAAATAG AACCTGCAGA TGCCATAAGC AACCAGAAAG CTGGTCTTAC TTCGGGCAAA 
CCTAAAAAAG ACAAGTCAGT CAAGGGGACG TCGCAAGCGG TTGTTTCAGA CACCAAGAAA 
ATGGAGGCCA GAAAGTCCTC CTCGGACACG GTGATCGAGC CATTGATCAA GCAGCAGGCG 
CCACTGCACT CGTCAAAGAC CACGCCAACG ACTTTGACGG CGAACTTCGT GCAAAACATT 
CGATTCGTCC GTAAAAATGT GGAACAGTCA GGCAGGAACA CCAATCCACT GCAGTTCGTT 
GAACTCGAGA CAGAGTTTCC TCGTGACTAT GACGACAACA TCGAGATGCT TTCCCGCGAG 
GCGGAGCATC TGGAGGAGCA GTTCCGCACG CCTACGCGCT CCAATGCTAC GGATGCCACT 
TCCCACCATG TGGCCGGAAT CATCGACAAC ATTATAACCG AAGCGTCACG GAGTCTTACA 
ATCGAAAAGA CTGAAGACGA TGTGCCATTG AAGTCTTCTA CCAAGCACTC AAGCGGCGTA 
AAACGAGTTG GCTTTCAAGT AGAGGATAAG GACGATACCG AAGTTCAGAG CGAAAAGCAG 
GCAAAGAGCT TCGACGACAT AACCAAAAAA GCGGAGAGCT CCGAGGCTAG CGCAGAGGAA 
GCTGCTGTCA CCGGGTCCTC GACAGATGCA TCTGCATCTC CCCTGCCCTC CACGTCGCTG 
GTCTCCACCA CATCTTCAGC TACATCGATA ACCAAGAGTA AGAGCGATGA GGACGATGAT 
CCGGTGGCCA TGTCGCCGTG CGGACGATTC TTTAAGTACG ATAAGGAAGT CGGACGCGGT 
TCCTTTAAAA CTGTCTATCG TGGCCTGGAC ACGTTGACTG GTGTTCCAGT TGCCTGGTGC 
GAGTTATTGG ACAAGCAAGT AAAAAAAAGT GAACGTACTA GATTTCGCGA GGAGGCAGAC 
ATGCTGAAGA AACTTCAGCA CCCAAACATA GTGCGGTTTT ATACATATTG GGAGTTTCCT 
ATTGGCCGCA AGAAGAATAT TGTACTAGTC ACCGAACTAA TGTTATCTGG AACTTTGAAA 
TCCTATTTGA AACGATTCAA AAAAATACAC CCAAAGGTAT TGAAGTCATG GTGTCGCCAG 
ATACTAAAAG GCCTTAACTT CCTGCATACT CGTCAATTTC CTATAATTCA TCGTGATTTG 
AAATGTGATA ATATTTTTAT AACTGGCACC ACTGGTAGTG TTAAAATCGG CGACTTGGGC 
CTGGCAACTT TGAAAAACCG CTCCCACGCG AAATCTGTGA TTGGTACGCC GGAGTTTATG 
GCCCCGGAAA TGTACGAGGA GCACTACGAC GAGTCGGTGG ACGTATATGC ATTCGGCATG 
TGCATGTTGG AGATGGCCAT TTCCGAATAT CCTTACAGTG AGTGCAAGGG TCCTGCACAG 
ATCTACAAAA AGGTGATATC GGGCATTAAG CCAGCGGCGT TGGCTAAGGT GGAGGATCCC 
AACGTGCGCG ACATTATCGA ACGGTGCATT GAACTTAAAA AGGAGGATCG CCCCAGCTGC 
AATGAACTGC TAGAATCGGA GTTCTTTGAC GAAGATATCG GTATACGAGT GGAGCCAACT 
GCTTCGGAGC AGTTCCTTTC TGATCCGAGC ATCAGCATCA TCGAGTTCCG ACTGCGGTTT 
ATGGATCCCA AGAAGCGCTC GTCCCGTCAT AAGGAAAACG AGGCAATTCA GTTTGAGTAC 
AACATCAGGC ATGACGAGTA CGAACAGATT GCGCAAGAAA TGATGAAGGA AAATATTATA 
TCTGAGGACG ACTCTCGCGC TGTAGCTCGG CTTTTAAAAG TGCAGGTGGT GTCGTTGCTT 
AAGGAACGTG CCCAGCGTCA AACTCAAATC AAGCTGCAAA ACGAGAAGTC TCGTTTGGAA 
AAATTGGCAT TGCAAAAGCA ACGTGAGAGT TTGCCGACTA ACGTCGATGA AGACGAGGAA 
GAGGAGGAGG AGTCCGAGGA CGAGGAGGAT GGTGTTAAAT GGAATCAGCG CCTGCAGCTA 
AGATATGACC TGCTTAACAC TGATTCAGAA ACAAGCCTGG CTCTATCCAC GAACAGCGTC 
GAACCGCAGC AGCTGTCCAC TCGCTCGAAC ACTTCGATTC CGAACAGTGG CATTCAGCAA 
CCTGTTCAGG TCCCAGGTCA AGTGCCTGTT TCCCAGTTGA TCTCGGTGCA ACCACAGGCT 
ATTCCGTCAC CGGCTATTCC CATGCAACAG AAGCCGACGG TGCACTACAT TCAACCGCCA 
CAGCTGGCCT CTTATCAAAA TTCGAATGCA TCGATGCAGG AGATGACCAA TAACCAGGTC 
ATTTCGCCGA CAGGCAGTCA ACAAATGCAA CAGCAACAAC CCGTAGTGGC ACCAACAGTC 
AATCATCAGG TCATGCCGCA GCAGCAAGTG AACCAACAAC AGCAACAACC ACAAATGATG 
CAGCAAATAC CCCAGCAAGT TCAAGTGCAA CAGCCCCAAA CTGTATTGCC TCCGCAGCCA 
CATGAGCAGC AGCCACAGCA ACAACAACAG CCACTGCAGC AACAACTGCA GCAGCTTATG 
CATACTAACG TGCAGGCTCC GGATTTGACT CAACAGCAAC AAATGGCTCA GCAACAGGCC 
CAGCAATATT TTCAGCAGCA GCAGCAACAG CCTCAGCAGG CCGTTAACAT GCAACAAGCT 
TATGCAATGC AGCAAGCTGG CCAACAGCAA CAGCTTTCGC AGCCTCTTCA AATTCAGCAA 
CAGATCTTGC AGCAACAACA AGTGGCCGTT TCACATCAGC AACAGATAAT GCAGCAACAA 
CTGGCCCAGC ATCAGCTGCA ACAGCTGCAG CAGCAGCAGC TTCAACAACA GCAACTGCAA 
CAGCAGCAGC AAATACAACA ACAGCAACTC CAACAACAAC AACTCCAGCA ACAGCAATTC 
GTACAGCAGT ATGCGCAGGC AATGCCTCAG CAACAACATC AGCAACTCGT TACGGGATCG 
CAAGTCATGG CACCGCATCA ACATCAGCAA CCTATTCAGA TCCCAGTGCA GATGCAAGTT 
CCACCGACGT CCGTGGCTCC TCCAATACAA CACACTTACA ATCAGCAGGG CGGACAAGTA 
ACGCTCAGTG ATGCCCAACA GCAACAGCAT CCTGGATTCT CGGCCGTACC ACAACAGGCA 
GCGCCATTTA TACAACAGCC CACACAGCAA CCCATTCAGC TATCAATGCC TTTGGAACAA 
CAATTGCAGC AATTGCTGCA TAGCCAGCCA GCCCAGCAAC AACAGGCCAT GAGCCAACAG 
CAGCAACAGC CCTTGGTGCA ACAGCAACAA CTGCCTTTGG TACAACAGCA GCCGCCTTTG 
GTCCAGCAGC AGCAACCGCT GGTTCAGCAT CAGCAGCCTT CGGTCCAACA TCAGCAACCT 
TTGGTGCAAC AGCCCCAGCA ACAGCAACCA CAGCCCCAGA ATCAGCAACC ACAGCCCCAG 
ACACAGCAAA CACACGTGGT TCAGCAACAA CCACCACAGC AGCAACCTGC AGTAGAGCAA 
ATCTCACAGA TTTCTTCGCA AGTGCCTGTT CAACAGGAAA ATCTGCAGCC AATACAGGTC 
AACAAAGATG CGAATGTCGC AACGGATGCA ATGTCCCTAA ATAGCGCCCA CGGTGCCCTC 
GAACCTGCAC CGAAGACCGA ACCCCAGAAC TCTGCTGATG CGGAAAAGCA GCAAAAGCAG 
ACAGGCACGG GCACACGTTC CCAGAAACCG AGGCGATCAA ACCGGAGCGG CAACGAACGG 
ATTCCCAAAC TTAGTGTAAC CAGCGTCGAC GAGGGCAGCG TCATAAACTG TCACATGGAA 
AATAAACTTA AAACGATTAC ATTTAAATTC GACATAGGAG ACGTAAATCC GGTTGAAATA 
GCCAATAAAT TGATCGCCCA AGATTTGCTT TCTAATTGTC AAAGTACAGT TTTTGTGGAA 
ATGATCAATG AAATTGTTGA CCAAGTCAAA CAGAATCCCA ATCAAATCCC TATACCGACA 
AATTATCGCC GCAATATTGA AAAGGTCCGA CACGCGTCTC TAACTCGACA GCGTTCCACG 
TTTAGGTCAC ACCAAAGACA TAGATCTCGG GATGAAACCG CCAGCGACAT CACAAAGATG 
TTCGAGCCCA CTATCCATGG TGTTGAAACC CTGCCATCCG GTGGAGGAGG CGCTGAACAG 
TCAAACTGCA ACTTGACTTT AGAGGCCAGG TCCCATTTAT CCAACATACC AAATGCGAAA 
GAACCACAGC TGAACGTCAG CACTCCGCCC ACAACCACTT CGACTATGTC TTCCTCATCA 
ACCGCATCGA GGGATGCGCC CAATAGTAGC AATGACGTGA CCATCGGCTC TGGTTCCGTT 
TCTCGAAAAA CGAGCACGGC CTCGGAGTAC ACTTCGCTAT CGATTGACTA TATGCCCGAC 
AGCAACATTA CTCCAACTGG TCCGGAGCCA CCGCTTGACG ATGGCAAGGA TAAAAAACCT 
TGCTCCCTTG CTATAGCCAG AATGCAAAAG CTCTTGGAGT CGGCCGGCAA TGGTGCTCCT 
CGCAGCCTGA ATTTGCCCTT GAATCGTCAC CTGAAAATTC AAGAGGACTT GAAGCATACT 
AGAAGTCTTG ACGATTTGAC CGCAGTGAAA ATAACCTTTG ACATGCCCAA CAAGGCTGCA 
TTGGACACTT CGGAAAACGC TCAGCAGGCC ACAGAAGCGG AAGCGGAGAA ACCAAAAGAT 
AAATCCGGAC AGGCAGTAGG CAACCAGGGA ACTGGAGCGG CCAACACTCT AGAGCAGTTA 
AAAATAGAAC TGGAGAACAT TACTCATGCC CACGCGTTTG CCTCCGCTGT AGTGGCCTCG 
ATTAACAACA GAGCTCCTCA TCAAGCTTCC CCAGCAATGT CCTCCCTAAA GGCCACTTCT 
GGACAACCAC AGACACAAGA GATAACCAAG CCAAATAACG GAGCAGGCCC TTCAGTACCC 
TCAGTGGGTC AGAATACTCC CACTGCAGCC TTAACATCCG CCAGGGGCTC AGGCTCCTCC 
GTCTACAACT CACGTCGCAC ATCCATCGAC AACAGCGTGG GATCCGACAT GCACCTGCAC 
ACTGCCACTA ATCTTGAAGG GACAGTGAGC AACCCGGATC CGATCCCGAC TGAGGCATCG 
GTTGGAATTA CCATTGCTAC GGGTCATGAG AAGCAACTCT CCAAGCAGCC CAGTTTGGAA 
AAGCCTTCTG CCACTTCCAT TTTAACGAAC AACAGTGATC CTCCGCAAAG GAATCCCAGC 
AATGGATCAA TTAACCAAAA TTCAATAGCT GATTTGGAAA AGAAATTGGC AGCTTTGCGA 
AATACCGAAA ACACAGAAGA GTCTGCAACA GCAACGTTGT CAGTGGTTCC TAAACAAGTT 
GAAGAGGTAG TCAATCCAAG CGCGCGTAAA ATTTCGCGTT TTAGTGTCAG TCGTGTGCAG 
GAGCAGAAAA CTTCAACCGG AGTTGAGGAA CCAGCTCAAG GTCAACTTAA GATCGACCTC 
CAAGTCGCCG GCCCTGGAGG CCAGGTGCAG ACCAACAACT CCGTTCAGAA CGGCAGCGTG 
GTCAACACTC CAACCGAAGT CATCAGCTCT CCTATTCAAA ATGTTCCGCT GGCCATTAAC 
GGAATTCAGT TGATGTACCA ACAGCCCCAG CAGATACACC AGTTGCCAGG TGGAACATCA 
ACTACTACAA GTGGAGCAGG GACTCAGTGC ATTGTAGTGC CGCAAGTGGT CAATCAAAAT 
GGCACCCACG TGCAACAGCC GTCTAACCTA CAGCCACAGC AGCAGAGCGT ACATCCAAAT 
ATGACTCAAC AGCCACAGCA GACGCCACTT AATGGGCACC CATCTATGGT TAACACTCTT 
CAGCAGCAGC CACCACAACA ATCCTTACCG ATGCAGACGA TTCAGTCGCA GCAGCAGCAG 
CATAACCAAA TGCCTATCAT TTCGCAACAA CAGCAGCAGC AGATCCTAAT GCAGCAGCAA 
CAGCAACAGG GATCCCAGCA AGGATCACAG CAATTTAATT TGCCTGGCAC ACAACAGACT 
CATCCTCAGC ACCAGTTCAT CCAGTCGCAG CCCAACCAAC TTCACTCGCT TCCGCCCCAG 
GTAGTGAGCA TGGCTCAGGG AAATCTGCCG CATCAGCTTC CACCCATGCA GACTATGGGA 
GCCCAACAGC AAATGATCTC GCAACAACAG CATCAGTTGC AGATGCAGTC GCATATGCAC 
CAACAAAACG TGCCCGGAAT GTACAACCAG CAGACTGGAG CTCGTGTGGC AGCACCCCAA 
AATTTCGCAG GTGCAGTTCC AAATCATTTG TTGCAGCAGT CCCCTTTGAT GGCATCCCAG 
CAGCCAGCAC AGCCCATGCA ACACGTTATG CAACCGATTT TCAATGCAAC GTCCGGTGAA 
GTTACCGAAG AGCCTGTCTC TTTAGCAGCT ACGCATCCGC ATCTGCTACC GAGCGATATT 
CAATCCGATA TAAAGCACAA TTTAGACTCA TTAGTGAACC AATTGTGCAA TACGCGCTTG 
GGTACTAACC AGCACCAACG GCTGTTGCTG TTGCGTCAGA GACAACTCAT CGAAGAGGAC 
GAGCTGCGTC TTAAACATTA TGTGGAATAC GAAAAGTTCC AAAAGGCACT GCGCCAATCT 
ATTAGCACAA ACGTTCCAGC TAATGCAGCT TACTATTCTG CGGCTGCCGC ACAGCTTCCA 
ACTAATTTGG CAGCCCCAGC AGCTCCGTCC TCATCGAATC CGACGTCCAC TAGCTCCGCA 
AACACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262171.2
CDS	413..7801
Translation	IGQTLCDFVRQQEVVPANRARKGSAISEDGTTSCTTQPQRNTSISSEEQTVQGANIQKSGHRSFNRSASSRQKPNPTSKLKDTLNRKPTCKQGTLSAHTSTSSTTSIQSSPIEPASSLPTLNTTPTPPAASKSNLFVRVLRRFSNNTLPGKTASSPKNVDDVPKVNDNNNGDLGKQQSTDVEVLCSQDNQSREQNEDEMDSKIEPADAISNQKAGLTSGKPKKDKSVKGTSQAVVSDTKKMEARKSSSDTVIEPLIKQQAPLHSSKTTPTTLTANFVQNIRFVRKNVEQSGRNTNPLQFVELETEFPRDYDDNIEMLSREAEHLEEQFRTPTRSNATDATSHHVAGIIDNIITEASRSLTIEKTEDDVPLKSSTKHSSGVKRVGFQVEDKDDTEVQSEKQAKSFDDITKKAESSEASAEEAAVTGSSTDASASPLPSTSLVSTTSSATSITKSKSDEDDDPVAMSPCGRFFKYDKEVGRGSFKTVYRGLDTLTGVPVAWCELLDKQVKKSERTRFREEADMLKKLQHPNIVRFYTYWEFPIGRKKNIVLVTELMLSGTLKSYLKRFKKIHPKVLKSWCRQILKGLNFLHTRQFPIIHRDLKCDNIFITGTTGSVKIGDLGLATLKNRSHAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMAISEYPYSECKGPAQIYKKVISGIKPAALAKVEDPNVRDIIERCIELKKEDRPSCNELLESEFFDEDIGIRVEPTASEQFLSDPSISIIEFRLRFMDPKKRSSRHKENEAIQFEYNIRHDEYEQIAQEMMKENIISEDDSRAVARLLKVQVVSLLKERAQRQTQIKLQNEKSRLEKLALQKQRESLPTNVDEDEEEEEESEDEEDGVKWNQRLQLRYDLLNTDSETSLALSTNSVEPQQLSTRSNTSIPNSGIQQPVQVPGQVPVSQLISVQPQAIPSPAIPMQQKPTVHYIQPPQLASYQNSNASMQEMTNNQVISPTGSQQMQQQQPVVAPTVNHQVMPQQQVNQQQQQPQMMQQIPQQVQVQQPQTVLPPQPHEQQPQQQQQPLQQQLQQLMHTNVQAPDLTQQQQMAQQQAQQYFQQQQQQPQQAVNMQQAYAMQQAGQQQQLSQPLQIQQQILQQQQVAVSHQQQIMQQQLAQHQLQQLQQQQLQQQQLQQQQQIQQQQLQQQQLQQQQFVQQYAQAMPQQQHQQLVTGSQVMAPHQHQQPIQIPVQMQVPPTSVAPPIQHTYNQQGGQVTLSDAQQQQHPGFSAVPQQAAPFIQQPTQQPIQLSMPLEQQLQQLLHSQPAQQQQAMSQQQQQPLVQQQQLPLVQQQPPLVQQQQPLVQHQQPSVQHQQPLVQQPQQQQPQPQNQQPQPQTQQTHVVQQQPPQQQPAVEQISQISSQVPVQQENLQPIQVNKDANVATDAMSLNSAHGALEPAPKTEPQNSADAEKQQKQTGTGTRSQKPRRSNRSGNERIPKLSVTSVDEGSVINCHMENKLKTITFKFDIGDVNPVEIANKLIAQDLLSNCQSTVFVEMINEIVDQVKQNPNQIPIPTNYRRNIEKVRHASLTRQRSTFRSHQRHRSRDETASDITKMFEPTIHGVETLPSGGGGAEQSNCNLTLEARSHLSNIPNAKEPQLNVSTPPTTTSTMSSSSTASRDAPNSSNDVTIGSGSVSRKTSTASEYTSLSIDYMPDSNITPTGPEPPLDDGKDKKPCSLAIARMQKLLESAGNGAPRSLNLPLNRHLKIQEDLKHTRSLDDLTAVKITFDMPNKAALDTSENAQQATEAEAEKPKDKSGQAVGNQGTGAANTLEQLKIELENITHAHAFASAVVASINNRAPHQASPAMSSLKATSGQPQTQEITKPNNGAGPSVPSVGQNTPTAALTSARGSGSSVYNSRRTSIDNSVGSDMHLHTATNLEGTVSNPDPIPTEASVGITIATGHEKQLSKQPSLEKPSATSILTNNSDPPQRNPSNGSINQNSIADLEKKLAALRNTENTEESATATLSVVPKQVEEVVNPSARKISRFSVSRVQEQKTSTGVEEPAQGQLKIDLQVAGPGGQVQTNNSVQNGSVVNTPTEVISSPIQNVPLAINGIQLMYQQPQQIHQLPGGTSTTTSGAGTQCIVVPQVVNQNGTHVQQPSNLQPQQQSVHPNMTQQPQQTPLNGHPSMVNTLQQQPPQQSLPMQTIQSQQQQHNQMPIISQQQQQQILMQQQQQQGSQQGSQQFNLPGTQQTHPQHQFIQSQPNQLHSLPPQVVSMAQGNLPHQLPPMQTMGAQQQMISQQQHQLQMQSHMHQQNVPGMYNQQTGARVAAPQNFAGAVPNHLLQQSPLMASQQPAQPMQHVMQPIFNATSGEVTEEPVSLAATHPHLLPSDIQSDIKHNLDSLVNQLCNTRLGTNQHQRLLLLRQRQLIEEDELRLKHYVEYEKFQKALRQSISTNVPANAAYYSAAAAQLPTNLAAPAAPSSSNPTSTSSANT

Target ORF information:

RefSeq Version	NM_001275242.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Wnk kinase (Wnk), transcript variant M, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275242.2

ORF Insert Sequence:

ATTGGACAAA CCCTCTGCGA CTTCGTACGA CAACAAGAGG TCGTACCTGC GAACCGTGCT 
CGGAAGGGAA GCGCCATCAG CGAAGATGGT ACTACCTCCT GCACCACGCA ACCTCAAAGG 
AATACCTCAA TTTCTAGCGA GGAGCAAACT GTGCAGGGAG CTAATATACA AAAGTCGGGA 
CACCGTTCGT TTAATAGATC TGCCTCCTCT AGGCAAAAGC CGAACCCGAC ATCAAAACTA 
AAAGACACAC TAAACAGAAA GCCCACTTGC AAGCAGGGCA CACTTTCTGC ACACACGAGC 
ACTAGCAGCA CCACTTCAAT CCAGTCATCA CCTATCGAGC CAGCTTCTTC TCTACCCACG 
CTGAACACCA CGCCTACACC ACCAGCTGCC AGCAAAAGTA ACCTATTTGT GCGGGTGCTA 
AGACGCTTTT CAAATAACAC CCTTCCCGGC AAAACGGCGT CAAGCCCGAA AAACGTGGAC 
GACGTACCGA AAGTCAACGA TAACAACAAC GGCGATTTAG GCAAACAGCA ATCAACCGAT 
GTAGAGGTGC TTTGCTCTCA GGACAACCAG AGCAGGGAAC AGAATGAGGA CGAAATGGAT 
AGCAAAATAG AACCTGCAGA TGCCATAAGC AACCAGAAAG CTGGTCTTAC TTCGGGCAAA 
CCTAAAAAAG ACAAGTCAGT CAAGGGGACG TCGCAAGCGG TTGTTTCAGA CACCAAGAAA 
ATGGAGGCCA GAAAGTCCTC CTCGGACACG GTGATCGAGC CATTGATCAA GCAGCAGGCG 
CCACTGCACT CGTCAAAGAC CACGCCAACG ACTTTGACGG CGAACTTCGT GCAAAACATT 
CGATTCGTCC GTAAAAATGT GGAACAGTCA GGCAGGAACA CCAATCCACT GCAGTTCGTT 
GAACTCGAGA CAGAGTTTCC TCGTGACTAT GACGACAACA TCGAGATGCT TTCCCGCGAG 
GCGGAGCATC TGGAGGAGCA GTTCCGCACG CCTACGCGCT CCAATGCTAC GGATGCCACT 
TCCCACCATG TGGCCGGAAT CATCGACAAC ATTATAACCG AAGCGTCACG GAGTCTTACA 
ATCGAAAAGA CTGAAGACGA TGTGCCATTG AAGTCTTCTA CCAAGCACTC AAGCGGCGTA 
AAACGAGTTG GCTTTCAAGT AGAGGATAAG GACGATACCG AAGTTCAGAG CGAAAAGCAG 
GCAAAGAGCT TCGACGACAT AACCAAAAAA GCGGAGAGCT CCGAGGCTAG CGCAGAGGAA 
GCTGCTGTCA CCGGGTCCTC GACAGATGCA TCTGCATCTC CCCTGCCCTC CACGTCGCTG 
GTCTCCACCA CATCTTCAGC TACATCGATA ACCAAGAGTA AGAGCGATGA GGACGATGAT 
CCGGTGGCCA TGTCGCCGTG CGGACGATTC TTTAAGTACG ATAAGGAAGT CGGACGCGGT 
TCCTTTAAAA CTGTCTATCG TGGCCTGGAC ACGTTGACTG GTGTTCCAGT TGCCTGGTGC 
GAGTTATTGG ACAAGCAAGT AAAAAAAAGT GAACGTACTA GATTTCGCGA GGAGGCAGAC 
ATGCTGAAGA AACTTCAGCA CCCAAACATA GTGCGGTTTT ATACATATTG GGAGTTTCCT 
ATTGGCCGCA AGAAGAATAT TGTACTAGTC ACCGAACTAA TGTTATCTGG AACTTTGAAA 
TCCTATTTGA AACGATTCAA AAAAATACAC CCAAAGGTAT TGAAGTCATG GTGTCGCCAG 
ATACTAAAAG GCCTTAACTT CCTGCATACT CGTCAATTTC CTATAATTCA TCGTGATTTG 
AAATGTGATA ATATTTTTAT AACTGGCACC ACTGGTAGTG TTAAAATCGG CGACTTGGGC 
CTGGCAACTT TGAAAAACCG CTCCCACGCG AAATCTGTGA TTGGTACGCC GGAGTTTATG 
GCCCCGGAAA TGTACGAGGA GCACTACGAC GAGTCGGTGG ACGTATATGC ATTCGGCATG 
TGCATGTTGG AGATGGCCAT TTCCGAATAT CCTTACAGTG AGTGCAAGGG TCCTGCACAG 
ATCTACAAAA AGGTGATATC GGGCATTAAG CCAGCGGCGT TGGCTAAGGT GGAGGATCCC 
AACGTGCGCG ACATTATCGA ACGGTGCATT GAACTTAAAA AGGAGGATCG CCCCAGCTGC 
AATGAACTGC TAGAATCGGA GTTCTTTGAC GAAGATATCG GTATACGAGT GGAGCCAACT 
GCTTCGGAGC AGTTCCTTTC TGATCCGAGC ATCAGCATCA TCGAGTTCCG ACTGCGGTTT 
ATGGATCCCA AGAAGCGCTC GTCCCGTCAT AAGGAAAACG AGGCAATTCA GTTTGAGTAC 
AACATCAGGC ATGACGAGTA CGAACAGATT GCGCAAGAAA TGATGAAGGA AAATATTATA 
TCTGAGGACG ACTCTCGCGC TGTAGCTCGG CTTTTAAAAG TGCAGGTGGT GTCGTTGCTT 
AAGGAACGTG CCCAGCGTCA AACTCAAATC AAGCTGCAAA ACGAGAAGTC TCGTTTGGAA 
AAATTGGCAT TGCAAAAGCA ACGTGAGAGT TTGCCGACTA ACGTCGATGA AGACGAGGAA 
GAGGAGGAGG AGTCCGAGGA CGAGGAGGAT GGTGTTAAAT GGAATCAGCG CCTGCAGCTA 
AGATATGACC TGCTTAACAC TGATTCAGAA ACAAGCCTGG CTCTATCCAC GAACAGCGTC 
GAACCGCAGC AGCTGTCCAC TCGCTCGAAC ACTTCGATTC CGAACAGTGG CATTCAGCAA 
CCTGTTCAGG TCCCAGGTCA AGTGCCTGTT TCCCAGTTGA TCTCGGTGCA ACCACAGGCT 
ATTCCGTCAC CGGCTATTCC CATGCAACAG AAGCCGACGG TGCACTACAT TCAACCGCCA 
CAGCTGGCCT CTTATCAAAA TTCGAATGCA TCGATGCAGG AGATGACCAA TAACCAGGTC 
ATTTCGCCGA CAGGCAGTCA ACAAATGCAA CAGCAACAAC CCGTAGTGGC ACCAACAGTC 
AATCATCAGG TCATGCCGCA GCAGCAAGTG AACCAACAAC AGCAACAACC ACAAATGATG 
CAGCAAATAC CCCAGCAAGT TCAAGTGCAA CAGCCCCAAA CTGTATTGCC TCCGCAGCCA 
CATGAGCAGC AGCCACAGCA ACAACAACAG CCACTGCAGC AACAACTGCA GCAGCTTATG 
CATACTAACG TGCAGGCTCC GGATTTGACT CAACAGCAAC AAATGGCTCA GCAACAGGCC 
CAGCAATATT TTCAGCAGCA GCAGCAACAG CCTCAGCAGG CCGTTAACAT GCAACAAGCT 
TATGCAATGC AGCAAGCTGG CCAACAGCAA CAGCTTTCGC AGCCTCTTCA AATTCAGCAA 
CAGATCTTGC AGCAACAACA AGTGGCCGTT TCACATCAGC AACAGATAAT GCAGCAACAA 
CTGGCCCAGC ATCAGCTGCA ACAGCTGCAG CAGCAGCAGC TTCAACAACA GCAACTGCAA 
CAGCAGCAGC AAATACAACA ACAGCAACTC CAACAACAAC AACTCCAGCA ACAGCAATTC 
GTACAGCAGT ATGCGCAGGC AATGCCTCAG CAACAACATC AGCAACTCGT TACGGGATCG 
CAAGTCATGG CACCGCATCA ACATCAGCAA CCTATTCAGA TCCCAGTGCA GATGCAAGTT 
CCACCGACGT CCGTGGCTCC TCCAATACAA CACACTTACA ATCAGCAGGG CGGACAAGTA 
ACGCTCAGTG ATGCCCAACA GCAACAGCAT CCTGGATTCT CGGCCGTACC ACAACAGGCA 
GCGCCATTTA TACAACAGCC CACACAGCAA CCCATTCAGC TATCAATGCC TTTGGAACAA 
CAATTGCAGC AATTGCTGCA TAGCCAGCCA GCCCAGCAAC AACAGGCCAT GAGCCAACAG 
CAGCAACAGC CCTTGGTGCA ACAGCAACAA CTGCCTTTGG TACAACAGCA GCCGCCTTTG 
GTCCAGCAGC AGCAACCGCT GGTTCAGCAT CAGCAGCCTT CGGTCCAACA TCAGCAACCT 
TTGGTGCAAC AGCCCCAGCA ACAGCAACCA CAGCCCCAGA ATCAGCAACC ACAGCCCCAG 
ACACAGCAAA CACACGTGGT TCAGCAACAA CCACCACAGC AGCAACCTGC AGTAGAGCAA 
ATCTCACAGA TTTCTTCGCA AGTGCCTGTT CAACAGGAAA ATCTGCAGCC AATACAGGTC 
AACAAAGATG CGAATGTCGC AACGGATGCA ATGTCCCTAA ATAGCGCCCA CGGTGCCCTC 
GAACCTGCAC CGAAGACCGA ACCCCAGAAC TCTGCTGATG CGGAAAAGCA GCAAAAGCAG 
ACAGGCACGG GCACACGTTC CCAGAAACCG AGGCGATCAA ACCGGAGCGG CAACGAACGG 
ATTCCCAAAC TTAGTGTAAC CAGCGTCGAC GAGGGCAGCG TCATAAACTG TCACATGGAA 
AATAAACTTA AAACGATTAC ATTTAAATTC GACATAGGAG ACGTAAATCC GGTTGAAATA 
GCCAATAAAT TGATCGCCCA AGATTTGCTT TCTAATTGTC AAAGTACAGT TTTTGTGGAA 
ATGATCAATG AAATTGTTGA CCAAGTCAAA CAGAATCCCA ATCAAATCCC TATACCGACA 
AATTATCGCC GCAATATTGA AAAGGTCCGA CACGCGTCTC TAACTCGACA GCGTTCCACG 
TTTAGGTCAC ACCAAAGACA TAGATCTCGG GATGAAACCG CCAGCGACAT CACAAAGATG 
TTCGAGCCCA CTATCCATGG TGTTGAAACC CTGCCATCCG GTGGAGGAGG CGCTGAACAG 
TCAAACTGCA ACTTGACTTT AGAGGCCAGG TCCCATTTAT CCAACATACC AAATGCGAAA 
GAACCACAGC TGAACGTCAG CACTCCGCCC ACAACCACTT CGACTATGTC TTCCTCATCA 
ACCGCATCGA GGGATGCGCC CAATAGTAGC AATGACGTGA CCATCGGCTC TGGTTCCGTT 
TCTCGAAAAA CGAGCACGGC CTCGGAGTAC ACTTCGCTAT CGATTGACTA TATGCCCGAC 
AGCAACATTA CTCCAACTGG TCCGGAGCCA CCGCTTGACG ATGGCAAGGA TAAAAAACCT 
TGCTCCCTTG CTATAGCCAG AATGCAAAAG CTCTTGGAGT CGGCCGGCAA TGGTGCTCCT 
CGCAGCCTGA ATTTGCCCTT GAATCGTCAC CTGAAAATTC AAGAGGACTT GAAGCATACT 
AGAAGTCTTG ACGATTTGAC CGCAGTGAAA ATAACCTTTG ACATGCCCAA CAAGGCTGCA 
TTGGACACTT CGGAAAACGC TCAGCAGGCC ACAGAAGCGG AAGCGGAGAA ACCAAAAGAT 
AAATCCGGAC AGGCAGTAGG CAACCAGGGA ACTGGAGCGG CCAACACTCT AGAGCAGTTA 
AAAATAGAAC TGGAGAACAT TACTCATGCC CACGCGTTTG CCTCCGCTGT AGTGGCCTCG 
ATTAACAACA GAGCTCCTCA TCAAGCTTCC CCAGCAATGT CCTCCCTAAA GGCCACTTCT 
GGACAACCAC AGACACAAGA GATAACCAAG CCAAATAACG GAGCAGGCCC TTCAGTACCC 
TCAGTGGGTC AGAATACTCC CACTGCAGCC TTAACATCCG CCAGGGGCTC AGGCTCCTCC 
GTCTACAACT CACGTCGCAC ATCCATCGAC AACAGCGTGG GATCCGACAT GCACCTGCAC 
ACTGCCACTA ATCTTGAAGG GACAGTGAGC AACCCGGATC CGATCCCGAC TGAGGCATCG 
GTTGGAATTA CCATTGCTAC GGGTCATGAG AAGCAACTCT CCAAGCAGCC CAGTTTGGAA 
AAGCCTTCTG CCACTTCCAT TTTAACGAAC AACAGTGATC CTCCGCAAAG GAATCCCAGC 
AATGGATCAA TTAACCAAAA TTCAATAGCT GATTTGGAAA AGAAATTGGC AGCTTTGCGA 
AATACCGAAA ACACAGAAGA GTCTGCAACA GCAACGTTGT CAGTGGTTCC TAAACAAGTT 
GAAGAGGTAG TCAATCCAAG CGCGCGTAAA ATTTCGCGTT TTAGTGTCAG TCGTGTGCAG 
GAGCAGAAAA CTTCAACCGG AGTTGAGGAA CCAGCTCAAG GTCAACTTAA GATCGACCTC 
CAAGTCGCCG GCCCTGGAGG CCAGGTGCAG ACCAACAACT CCGTTCAGAA CGGCAGCGTG 
GTCAACACTC CAACCGAAGT CATCAGCTCT CCTATTCAAA ATGTTCCGCT GGCCATTAAC 
GGAATTCAGT TGATGTACCA ACAGCCCCAG CAGATACACC AGTTGCCAGG TGGAACATCA 
ACTACTACAA GTGGAGCAGG GACTCAGTGC ATTGTAGTGC CGCAAGTGGT CAATCAAAAT 
GGCACCCACG TGCAACAGCC GTCTAACCTA CAGCCACAGC AGCAGAGCGT ACATCCAAAT 
ATGACTCAAC AGCCACAGCA GACGCCACTT AATGGGCACC CATCTATGGT TAACACTCTT 
CAGCAGCAGC CACCACAACA ATCCTTACCG ATGCAGACGA TTCAGTCGCA GCAGCAGCAG 
CATAACCAAA TGCCTATCAT TTCGCAACAA CAGCAGCAGC AGATCCTAAT GCAGCAGCAA 
CAGCAACAGG GATCCCAGCA AGGATCACAG CAATTTAATT TGCCTGGCAC ACAACAGACT 
CATCCTCAGC ACCAGTTCAT CCAGTCGCAG CCCAACCAAC TTCACTCGCT TCCGCCCCAG 
GTAGTGAGCA TGGCTCAGGG AAATCTGCCG CATCAGCTTC CACCCATGCA GACTATGGGA 
GCCCAACAGC AAATGATCTC GCAACAACAG CATCAGTTGC AGATGCAGTC GCATATGCAC 
CAACAAAACG TGCCCGGAAT GTACAACCAG CAGACTGGAG CTCGTGTGGC AGCACCCCAA 
AATTTCGCAG GTGCAGTTCC AAATCATTTG TTGCAGCAGT CCCCTTTGAT GGCATCCCAG 
CAGCCAGCAC AGCCCATGCA ACACGTTATG CAACCGATTT TCAATGCAAC GTCCGGTGAA 
GTTACCGAAG AGCCTGTCTC TTTAGCAGCT ACGCATCCGC ATCTGCTACC GAGCGATATT 
CAATCCGATA TAAAGCACAA TTTAGACTCA TTAGTGAACC AATTGTGCAA TACGCGCTTG 
GGTACTAACC AGCACCAACG GCTGTTGCTG TTGCGTCAGA GACAACTCAT CGAAGAGGAC 
GAGCTGCGTC TTAAACATTA TGTGGAATAC GAAAAGTTCC AAAAGGCACT GCGCCAATCT 
ATTAGCACAA ACGTTCCAGC TAATGCAGCT TACTATTCTG CGGCTGCCGC ACAGCTTCCA 
ACTAATTTGG CAGCCCCAGC AGCTCCGTCC TCATCGAATC CGACGTCCAC TAGCTCCGCA 
AACACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wnk ( NM_001275244.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262173.2 Drosophila melanogaster Wnk kinase (Wnk), transcript variant O, mRNA.

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CloneID	OFa41369
Clone ID Related Accession (Same CDS sequence)	NM_001275244.2
Accession Version	NM_001275244.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Wnk kinase, isoform O
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Oct 19, 2015 this sequence version replaced NM_001275244.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAGCA AAATAGAACC TGCAGATGCC ATAAGCAACC AGAAAGCTGG TCTTACTTCG 
GGCAAACCTA AAAAAGACAA GTCAGTCAAG GGGACGTCGC AAGCGGTTGT TTCAGACACC 
AAGAAAATGG AGGCCAGAAA GTCCTCCTCG GACACGGTGA TCGAGCCATT GATCAAGCAG 
CAGGCGCCAC TGCACTCGTC AAAGACCACG CCAACGACTT TGACGGCGAA CTTCGTGCAA 
AACATTCGAT TCGTCCGTAA AAATGTGGAA CAGTCAGGCA GGAACACCAA TCCACTGCAG 
TTCGTTGAAC TCGAGACAGA GTTTCCTCGT GACTATGACG ACAACATCGA GATGCTTTCC 
CGCGAGGCGG AGCATCTGGA GGAGCAGTTC CGCACGCCTA CGCGCTCCAA TGCTACGGAT 
GCCACTTCCC ACCATGTGGC CGGAATCATC GACAACATTA TAACCGAAGC GTCACGGAGT 
CTTACAATCG AAAAGACTGA AGACGATGTG CCATTGAAGT CTTCTACCAA GCACTCAAGC 
GGCGTAAAAC GAGTTGGCTT TCAAGTAGAG GATAAGGACG ATACCGAAGT TCAGAGCGAA 
AAGCAGGCAA AGAGCTTCGA CGACATAACC AAAAAAGCGG AGAGCTCCGA GGCTAGCGCA 
GAGGAAGCTG CTGTCACCGG GTCCTCGACA GATGCATCTG CATCTCCCCT GCCCTCCACG 
TCGCTGGTCT CCACCACATC TTCAGCTACA TCGATAACCA AGAGTAAGAG CGATGAGGAC 
GATGATCCGG TGGCCATGTC GCCGTGCGGA CGATTCTTTA AGTACGATAA GGAAGTCGGA 
CGCGGTTCCT TTAAAACTGT CTATCGTGGC CTGGACACGT TGACTGGTGT TCCAGTTGCC 
TGGTGCGAGT TATTGGACAA GCAAGTAAAA AAAAGTGAAC GTACTAGATT TCGCGAGGAG 
GCAGACATGC TGAAGAAACT TCAGCACCCA AACATAGTGC GGTTTTATAC ATATTGGGAG 
TTTCCTATTG GCCGCAAGAA GAATATTGTA CTAGTCACCG AACTAATGTT ATCTGGAACT 
TTGAAATCCT ATTTGAAACG ATTCAAAAAA ATACACCCAA AGGTATTGAA GTCATGGTGT 
CGCCAGATAC TAAAAGGCCT TAACTTCCTG CATACTCGTC AATTTCCTAT AATTCATCGT 
GATTTGAAAT GTGATAATAT TTTTATAACT GGCACCACTG GTAGTGTTAA AATCGGCGAC 
TTGGGCCTGG CAACTTTGAA AAACCGCTCC CACGCGAAAT CTGTGATTGG TACGCCGGAG 
TTTATGGCCC CGGAAATGTA CGAGGAGCAC TACGACGAGT CGGTGGACGT ATATGCATTC 
GGCATGTGCA TGTTGGAGAT GGCCATTTCC GAATATCCTT ACAGTGAGTG CAAGGGTCCT 
GCACAGATCT ACAAAAAGGT GATATCGGGC ATTAAGCCAG CGGCGTTGGC TAAGGTGGAG 
GATCCCAACG TGCGCGACAT TATCGAACGG TGCATTGAAC TTAAAAAGGA GGATCGCCCC 
AGCTGCAATG AACTGCTAGA ATCGGAGTTC TTTGACGAAG ATATCGGTAT ACGAGTGGAG 
CCAACTGCTT CGGAGCAGTT CCTTTCTGAT CCGAGCATCA GCATCATCGA GTTCCGACTG 
CGGTTTATGG ATCCCAAGAA GCGCTCGTCC CGTCATAAGG AAAACGAGGC AATTCAGTTT 
GAGTACAACA TCAGGCATGA CGAGTACGAA CAGATTGCGC AAGAAATGAT GAAGGAAAAT 
ATTATATCTG AGGACGACTC TCGCGCTGTA GCTCGGCTTT TAAAAGTGCA GGTGGTGTCG 
TTGCTTAAGG AACGTGCCCA GCGTCAAACT CAAATCAAGC TGCAAAACGA GAAGTCTCGT 
TTGGAAAAAT TGGCATTGCA AAAGCAACGT GAGAGTTTGC CGACTAACGT CGATGAAGAC 
GAGGAAGAGG AGGAGGAGTC CGAGGACGAG GAGGATGGTG TTAAATGGAA TCAGCGCCTG 
CAGCTAAGAT ATGACCTGCT TAACACTGAT TCAGAAACAA GCCTGGCTCT ATCCACGAAC 
AGCGTCGAAC CGCAGCAGCT GTCCACTCGC TCGAACACTT CGATTCCGAA CAGTGGCATT 
CAGCAACCTG TTCAGGTCCC AGGTCAAGTG CCTGTTTCCC AGTTGATCTC GGTGCAACCA 
CAGGCTATTC CGTCACCGGC TATTCCCATG CAACAGAAGC CGACGGTGCA CTACATTCAA 
CCGCCACAGC TGGCCTCTTA TCAAAATTCG AATGCATCGA TGCAGGAGAT GACCAATAAC 
CAGGTCATTT CGCCGACAGG CAGTCAACAA ATGCAACAGC AACAACCCGT AGTGGCACCA 
ACAGTCAATC ATCAGGTCAT GCCGCAGCAG CAAGTGAACC AACAACAGCA ACAACCACAA 
ATGATGCAGC AAATACCCCA GCAAGTTCAA GTGCAACAGC CCCAAACTGT ATTGCCTCCG 
CAGCCACATG AGCAGCAGCC ACAGCAACAA CAACAGCCAC TGCAGCAACA ACTGCAGCAG 
CTTATGCATA CTAACGTGCA GGCTCCGGAT TTGACTCAAC AGCAACAAAT GGCTCAGCAA 
CAGGCCCAGC AATATTTTCA GCAGCAGCAG CAACAGCCTC AGCAGGCCGT TAACATGCAA 
CAAGCTTATG CAATGCAGCA AGCTGGCCAA CAGCAACAGC TTTCGCAGCC TCTTCAAATT 
CAGCAACAGA TCTTGCAGCA ACAACAAGTG GCCGTTTCAC ATCAGCAACA GATAATGCAG 
CAACAACTGG CCCAGCATCA GCTGCAACAG CTGCAGCAGC AGCAGCTTCA ACAACAGCAA 
CTGCAACAGC AGCAGCAAAT ACAACAACAG CAACTCCAAC AACAACAACT CCAGCAACAG 
CAATTCGTAC AGCAGTATGC GCAGGCAATG CCTCAGCAAC AACATCAGCA ACTCGTTACG 
GGATCGCAAG TCATGGCACC GCATCAACAT CAGCAACCTA TTCAGATCCC AGTGCAGATG 
CAAGTTCCAC CGACGTCCGT GGCTCCTCCA ATACAACACA CTTACAATCA GCAGGGCGGA 
CAAGTAACGC TCAGTGATGC CCAACAGCAA CAGCATCCTG GATTCTCGGC CGTACCACAA 
CAGGCAGCGC CATTTATACA ACAGCCCACA CAGCAACCCA TTCAGCTATC AATGCCTTTG 
GAACAACAAT TGCAGCAATT GCTGCATAGC CAGCCAGCCC AGCAACAACA GGCCATGAGC 
CAACAGCAGC AACAGCCCTT GGTGCAACAG CAACAACTGC CTTTGGTACA ACAGCAGCCG 
CCTTTGGTCC AGCAGCAGCA ACCGCTGGTT CAGCATCAGC AGCCTTCGGT CCAACATCAG 
CAACCTTTGG TGCAACAGCC CCAGCAACAG CAACCACAGC CCCAGAATCA GCAACCACAG 
CCCCAGACAC AGCAAACACA CGTGGTTCAG CAACAACCAC CACAGCAGCA ACCTGCAGTA 
GAGCAAATCT CACAGATTTC TTCGCAAGTG CCTGTTCAAC AGGAAAATCT GCAGCCAATA 
CAGGTCAACA AAGATGCGAA TGTCGCAACG GATGCAATGT CCCTAAATAG CGCCCACGGT 
GCCCTCGAAC CTGCACCGAA GACCGAACCC CAGAACTCTG CTGATGCGGA AAAGCAGCAA 
AAGCAGACAG GCACGGGCAC ACGTTCCCAG AAACCGAGGC GATCAAACCG GAGCGGCAAC 
GAACGGATTC CCAAACTTAG TGTAACCAGC GTCGACGAGG GCAGCGTCAT AAACTGTCAC 
ATGGAAAATA AACTTAAAAC GATTACATTT AAATTCGACA TAGGAGACGT AAATCCGGTT 
GAAATAGCCA ATAAATTGAT CGCCCAAGAT TTGCTTTCTA ATTGTCAAAG TACAGTTTTT 
GTGGAAATGA TCAATGAAAT TGTTGACCAA GTCAAACAGA ATCCCAATCA AATCCCTATA 
CCGACAAATT ATCGCCGCAA TATTGAAAAG CGGGATGAAA CCGCCAGCGA CATCACAAAG 
ATGTTCGAGC CCACTATCCA TGGTGTTGAA ACCCTGCCAT CCGGTGGAGG AGGCGCTGAA 
CAGTCAAACT GCAACTTGAC TTTAGAGGCC AGGTCCCATT TATCCAACAT ACCAAATGCG 
AAAGAACCAC AGCTGAACGT CAGCACTCCG CCCACAACCA CTTCGACTAT GTCTTCCTCA 
TCAACCGCAT CGAGGGATGC GCCCAATAGT AGCAATGACG TGACCATCGG CTCTGGTTCC 
GTTTCTCGAA AAACGAGCAC GGCCTCGGAG TACACTTCGC TATCGATTGA CTATATGCCC 
GACAGCAACA TTACTCCAAC TGGTCCGGAG CCACCGCTTG ACGATGGCAA GGATAAAAAA 
CCTTGCTCCC TTGCTATAGC CAGAATGCAA AAGCTCTTGG AGTCGGCCGG CAATGGTGCT 
CCTCGCAGCC TGAATTTGCC CTTGAATCGT CACCTGAAAA TTCAAGAGGA CTTGAAGCAT 
ACTAGAAGTC TTGACGATTT GACCGCAGTG AAAATAACCT TTGACATGCC CAACAAGGCT 
GCATTGGACA CTTCGGAAAA CGCTCAGCAG GCCACAGAAG CGGAAGCGGA GAAACCAAAA 
GATAAATCCG GACAGGCAGT AGGCAACCAG GGAACTGGAG CGGCCAACAC TCTAGAGCAG 
TTAAAAATAG AACTGGAGAA CATTACTCAT GCCCACGCGT TTGCCTCCGC TGTAGTGGCC 
TCGATTAACA ACAGAGCTCC TCATCAAGCT TCCCCAGCAA TGTCCTCCCT AAAGGCCACT 
TCTGGACAAC CACAGACACA AGAGATAACC AAGCCAAATA ACGGAGCAGG CCCTTCAGTA 
CCCTCAGTGG GTCAGAATAC TCCCACTGCA GCCTTAACAT CCGCCAGGGG CTCAGGCTCC 
TCCGTCTACA ACTCACGTCG CACATCCATC GACAACAGCG TGGGATCCGA CATGCACCTG 
CACACTGCCA CTAATCTTGA AGGGACAGTG AGCAACCCGG ATCCGATCCC GACTGAGGCA 
TCGGTTGGAA TTACCATTGC TACGGGTCAT GAGAAGCAAC TCTCCAAGCA GCCCAGTTTG 
GAAAAGCCTT CTGCCACTTC CATTTTAACG AACAACAGTG ATCCTCCGCA AAGGAATCCC 
AGCAATGGAT CAATTAACCA AAATTCAATA GCTGATTTGG AAAAGAAATT GGCAGCTTTG 
CGAAATACCG AAAACACAGA AGAGTCTGCA ACAGCAACGT TGTCAGTGGT TCCTAAACAA 
GTTGAAGAGG TAGTCAATCC AAGCGCGCGT AAAATTTCGC GTTTTAGTGT CAGTCGTGTG 
CAGGAGCAGA AAACTTCAAC CGGAGTTGAG GAACCAGCTC AAGGTCAACT TAAGATCGAC 
CTCCAAGTCG CCGGCCCTGG AGGCCAGGTG CAGACCAACA ACTCCGTTCA GAACGGCAGC 
GTGGTCAACA CTCCAACCGA AGTCATCAGC TCTCCTATTC AAAATGTTCC GCTGGCCATT 
AACGGAATTC AGTTGATGTA CCAACAGCCC CAGCAGATAC ACCAGTTGCC AGGTGGAACA 
TCAACTACTA CAAGTGGAGC AGGGACTCAG TGCATTGTAG TGCCGCAAGT GGTCAATCAA 
AATGGCACCC ACGTGCAACA GCCGTCTAAC CTACAGCCAC AGCAGCAGAG CGTACATCCA 
AATATGACTC AACAGCCACA GCAGACGCCA CTTAATGGGC ACCCATCTAT GGTTAACACT 
CTTCAGCAGC AGCCACCACA ACAATCCTTA CCGATGCAGA CGATTCAGTC GCAGCAGCAG 
CAGCATAACC AAATGCCTAT CATTTCGCAA CAACAGCAGC AGCAGATCCT AATGCAGCAG 
CAACAGCAAC AGGGATCCCA GCAAGGATCA CAGCAATTTA ATTTGCCTGG CACACAACAG 
ACTCATCCTC AGCACCAGTT CATCCAGTCG CAGCCCAACC AACTTCACTC GCTTCCGCCC 
CAGGTAGTGA GCATGGCTCA GGGAAATCTG CCGCATCAGC TTCCACCCAT GCAGACTATG 
GGAGCCCAAC AGCAAATGAT CTCGCAACAA CAGCATCAGT TGCAGATGCA GTCGCATATG 
CACCAACAAA ACGTGCCCGG AATGTACAAC CAGCAGACTG GAGCTCGTGT GGCAGCACCC 
CAAAATTTCG CAGGTGCAGT TCCAAATCAT TTGTTGCAGC AGTCCCCTTT GATGGCATCC 
CAGCAGCCAG CACAGCCCAT GCAACACGTT ATGCAACCGA TTTTCAATGC AACGTCCGGT 
GAAGTTACCG AAGAGCCTGT CTCTTTAGCA GCTACGCATC CGCATCTGCT ACCGAGCGAT 
ATTCAATCCG ATATAAAGCA CAATTTAGAC TCATTAGTGA ACCAATTGTG CAATACGCGC 
TTGGGTACTA ACCAGCACCA ACGGCTGTTG CTGTTGCGTC AGAGACAACT CATCGAAGAG 
GACGAGCTGC GTCTTAAACA TTATGTGGAA TACGAAAAGT TCCAAAAGGC ACTGCGCCAA 
TCTATTAGCA CAAACGTTCC AGCTAATGCA GCTTACTATT CTGCGGCTGC CGCACAGCTT 
CCAACTAATT TGGCAGCCCC AGCAGCTCCG TCCTCATCGA ATCCGACGTC CACTAGCTCC 
GCAAACACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262173.2
CDS	1007..7738
Translation	MDSKIEPADAISNQKAGLTSGKPKKDKSVKGTSQAVVSDTKKMEARKSSSDTVIEPLIKQQAPLHSSKTTPTTLTANFVQNIRFVRKNVEQSGRNTNPLQFVELETEFPRDYDDNIEMLSREAEHLEEQFRTPTRSNATDATSHHVAGIIDNIITEASRSLTIEKTEDDVPLKSSTKHSSGVKRVGFQVEDKDDTEVQSEKQAKSFDDITKKAESSEASAEEAAVTGSSTDASASPLPSTSLVSTTSSATSITKSKSDEDDDPVAMSPCGRFFKYDKEVGRGSFKTVYRGLDTLTGVPVAWCELLDKQVKKSERTRFREEADMLKKLQHPNIVRFYTYWEFPIGRKKNIVLVTELMLSGTLKSYLKRFKKIHPKVLKSWCRQILKGLNFLHTRQFPIIHRDLKCDNIFITGTTGSVKIGDLGLATLKNRSHAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMAISEYPYSECKGPAQIYKKVISGIKPAALAKVEDPNVRDIIERCIELKKEDRPSCNELLESEFFDEDIGIRVEPTASEQFLSDPSISIIEFRLRFMDPKKRSSRHKENEAIQFEYNIRHDEYEQIAQEMMKENIISEDDSRAVARLLKVQVVSLLKERAQRQTQIKLQNEKSRLEKLALQKQRESLPTNVDEDEEEEEESEDEEDGVKWNQRLQLRYDLLNTDSETSLALSTNSVEPQQLSTRSNTSIPNSGIQQPVQVPGQVPVSQLISVQPQAIPSPAIPMQQKPTVHYIQPPQLASYQNSNASMQEMTNNQVISPTGSQQMQQQQPVVAPTVNHQVMPQQQVNQQQQQPQMMQQIPQQVQVQQPQTVLPPQPHEQQPQQQQQPLQQQLQQLMHTNVQAPDLTQQQQMAQQQAQQYFQQQQQQPQQAVNMQQAYAMQQAGQQQQLSQPLQIQQQILQQQQVAVSHQQQIMQQQLAQHQLQQLQQQQLQQQQLQQQQQIQQQQLQQQQLQQQQFVQQYAQAMPQQQHQQLVTGSQVMAPHQHQQPIQIPVQMQVPPTSVAPPIQHTYNQQGGQVTLSDAQQQQHPGFSAVPQQAAPFIQQPTQQPIQLSMPLEQQLQQLLHSQPAQQQQAMSQQQQQPLVQQQQLPLVQQQPPLVQQQQPLVQHQQPSVQHQQPLVQQPQQQQPQPQNQQPQPQTQQTHVVQQQPPQQQPAVEQISQISSQVPVQQENLQPIQVNKDANVATDAMSLNSAHGALEPAPKTEPQNSADAEKQQKQTGTGTRSQKPRRSNRSGNERIPKLSVTSVDEGSVINCHMENKLKTITFKFDIGDVNPVEIANKLIAQDLLSNCQSTVFVEMINEIVDQVKQNPNQIPIPTNYRRNIEKRDETASDITKMFEPTIHGVETLPSGGGGAEQSNCNLTLEARSHLSNIPNAKEPQLNVSTPPTTTSTMSSSSTASRDAPNSSNDVTIGSGSVSRKTSTASEYTSLSIDYMPDSNITPTGPEPPLDDGKDKKPCSLAIARMQKLLESAGNGAPRSLNLPLNRHLKIQEDLKHTRSLDDLTAVKITFDMPNKAALDTSENAQQATEAEAEKPKDKSGQAVGNQGTGAANTLEQLKIELENITHAHAFASAVVASINNRAPHQASPAMSSLKATSGQPQTQEITKPNNGAGPSVPSVGQNTPTAALTSARGSGSSVYNSRRTSIDNSVGSDMHLHTATNLEGTVSNPDPIPTEASVGITIATGHEKQLSKQPSLEKPSATSILTNNSDPPQRNPSNGSINQNSIADLEKKLAALRNTENTEESATATLSVVPKQVEEVVNPSARKISRFSVSRVQEQKTSTGVEEPAQGQLKIDLQVAGPGGQVQTNNSVQNGSVVNTPTEVISSPIQNVPLAINGIQLMYQQPQQIHQLPGGTSTTTSGAGTQCIVVPQVVNQNGTHVQQPSNLQPQQQSVHPNMTQQPQQTPLNGHPSMVNTLQQQPPQQSLPMQTIQSQQQQHNQMPIISQQQQQQILMQQQQQQGSQQGSQQFNLPGTQQTHPQHQFIQSQPNQLHSLPPQVVSMAQGNLPHQLPPMQTMGAQQQMISQQQHQLQMQSHMHQQNVPGMYNQQTGARVAAPQNFAGAVPNHLLQQSPLMASQQPAQPMQHVMQPIFNATSGEVTEEPVSLAATHPHLLPSDIQSDIKHNLDSLVNQLCNTRLGTNQHQRLLLLRQRQLIEEDELRLKHYVEYEKFQKALRQSISTNVPANAAYYSAAAAQLPTNLAAPAAPSSSNPTSTSSANT

Target ORF information:

RefSeq Version	NM_001275244.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Wnk kinase (Wnk), transcript variant O, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275244.2

ORF Insert Sequence:

ATGGATAGCA AAATAGAACC TGCAGATGCC ATAAGCAACC AGAAAGCTGG TCTTACTTCG 
GGCAAACCTA AAAAAGACAA GTCAGTCAAG GGGACGTCGC AAGCGGTTGT TTCAGACACC 
AAGAAAATGG AGGCCAGAAA GTCCTCCTCG GACACGGTGA TCGAGCCATT GATCAAGCAG 
CAGGCGCCAC TGCACTCGTC AAAGACCACG CCAACGACTT TGACGGCGAA CTTCGTGCAA 
AACATTCGAT TCGTCCGTAA AAATGTGGAA CAGTCAGGCA GGAACACCAA TCCACTGCAG 
TTCGTTGAAC TCGAGACAGA GTTTCCTCGT GACTATGACG ACAACATCGA GATGCTTTCC 
CGCGAGGCGG AGCATCTGGA GGAGCAGTTC CGCACGCCTA CGCGCTCCAA TGCTACGGAT 
GCCACTTCCC ACCATGTGGC CGGAATCATC GACAACATTA TAACCGAAGC GTCACGGAGT 
CTTACAATCG AAAAGACTGA AGACGATGTG CCATTGAAGT CTTCTACCAA GCACTCAAGC 
GGCGTAAAAC GAGTTGGCTT TCAAGTAGAG GATAAGGACG ATACCGAAGT TCAGAGCGAA 
AAGCAGGCAA AGAGCTTCGA CGACATAACC AAAAAAGCGG AGAGCTCCGA GGCTAGCGCA 
GAGGAAGCTG CTGTCACCGG GTCCTCGACA GATGCATCTG CATCTCCCCT GCCCTCCACG 
TCGCTGGTCT CCACCACATC TTCAGCTACA TCGATAACCA AGAGTAAGAG CGATGAGGAC 
GATGATCCGG TGGCCATGTC GCCGTGCGGA CGATTCTTTA AGTACGATAA GGAAGTCGGA 
CGCGGTTCCT TTAAAACTGT CTATCGTGGC CTGGACACGT TGACTGGTGT TCCAGTTGCC 
TGGTGCGAGT TATTGGACAA GCAAGTAAAA AAAAGTGAAC GTACTAGATT TCGCGAGGAG 
GCAGACATGC TGAAGAAACT TCAGCACCCA AACATAGTGC GGTTTTATAC ATATTGGGAG 
TTTCCTATTG GCCGCAAGAA GAATATTGTA CTAGTCACCG AACTAATGTT ATCTGGAACT 
TTGAAATCCT ATTTGAAACG ATTCAAAAAA ATACACCCAA AGGTATTGAA GTCATGGTGT 
CGCCAGATAC TAAAAGGCCT TAACTTCCTG CATACTCGTC AATTTCCTAT AATTCATCGT 
GATTTGAAAT GTGATAATAT TTTTATAACT GGCACCACTG GTAGTGTTAA AATCGGCGAC 
TTGGGCCTGG CAACTTTGAA AAACCGCTCC CACGCGAAAT CTGTGATTGG TACGCCGGAG 
TTTATGGCCC CGGAAATGTA CGAGGAGCAC TACGACGAGT CGGTGGACGT ATATGCATTC 
GGCATGTGCA TGTTGGAGAT GGCCATTTCC GAATATCCTT ACAGTGAGTG CAAGGGTCCT 
GCACAGATCT ACAAAAAGGT GATATCGGGC ATTAAGCCAG CGGCGTTGGC TAAGGTGGAG 
GATCCCAACG TGCGCGACAT TATCGAACGG TGCATTGAAC TTAAAAAGGA GGATCGCCCC 
AGCTGCAATG AACTGCTAGA ATCGGAGTTC TTTGACGAAG ATATCGGTAT ACGAGTGGAG 
CCAACTGCTT CGGAGCAGTT CCTTTCTGAT CCGAGCATCA GCATCATCGA GTTCCGACTG 
CGGTTTATGG ATCCCAAGAA GCGCTCGTCC CGTCATAAGG AAAACGAGGC AATTCAGTTT 
GAGTACAACA TCAGGCATGA CGAGTACGAA CAGATTGCGC AAGAAATGAT GAAGGAAAAT 
ATTATATCTG AGGACGACTC TCGCGCTGTA GCTCGGCTTT TAAAAGTGCA GGTGGTGTCG 
TTGCTTAAGG AACGTGCCCA GCGTCAAACT CAAATCAAGC TGCAAAACGA GAAGTCTCGT 
TTGGAAAAAT TGGCATTGCA AAAGCAACGT GAGAGTTTGC CGACTAACGT CGATGAAGAC 
GAGGAAGAGG AGGAGGAGTC CGAGGACGAG GAGGATGGTG TTAAATGGAA TCAGCGCCTG 
CAGCTAAGAT ATGACCTGCT TAACACTGAT TCAGAAACAA GCCTGGCTCT ATCCACGAAC 
AGCGTCGAAC CGCAGCAGCT GTCCACTCGC TCGAACACTT CGATTCCGAA CAGTGGCATT 
CAGCAACCTG TTCAGGTCCC AGGTCAAGTG CCTGTTTCCC AGTTGATCTC GGTGCAACCA 
CAGGCTATTC CGTCACCGGC TATTCCCATG CAACAGAAGC CGACGGTGCA CTACATTCAA 
CCGCCACAGC TGGCCTCTTA TCAAAATTCG AATGCATCGA TGCAGGAGAT GACCAATAAC 
CAGGTCATTT CGCCGACAGG CAGTCAACAA ATGCAACAGC AACAACCCGT AGTGGCACCA 
ACAGTCAATC ATCAGGTCAT GCCGCAGCAG CAAGTGAACC AACAACAGCA ACAACCACAA 
ATGATGCAGC AAATACCCCA GCAAGTTCAA GTGCAACAGC CCCAAACTGT ATTGCCTCCG 
CAGCCACATG AGCAGCAGCC ACAGCAACAA CAACAGCCAC TGCAGCAACA ACTGCAGCAG 
CTTATGCATA CTAACGTGCA GGCTCCGGAT TTGACTCAAC AGCAACAAAT GGCTCAGCAA 
CAGGCCCAGC AATATTTTCA GCAGCAGCAG CAACAGCCTC AGCAGGCCGT TAACATGCAA 
CAAGCTTATG CAATGCAGCA AGCTGGCCAA CAGCAACAGC TTTCGCAGCC TCTTCAAATT 
CAGCAACAGA TCTTGCAGCA ACAACAAGTG GCCGTTTCAC ATCAGCAACA GATAATGCAG 
CAACAACTGG CCCAGCATCA GCTGCAACAG CTGCAGCAGC AGCAGCTTCA ACAACAGCAA 
CTGCAACAGC AGCAGCAAAT ACAACAACAG CAACTCCAAC AACAACAACT CCAGCAACAG 
CAATTCGTAC AGCAGTATGC GCAGGCAATG CCTCAGCAAC AACATCAGCA ACTCGTTACG 
GGATCGCAAG TCATGGCACC GCATCAACAT CAGCAACCTA TTCAGATCCC AGTGCAGATG 
CAAGTTCCAC CGACGTCCGT GGCTCCTCCA ATACAACACA CTTACAATCA GCAGGGCGGA 
CAAGTAACGC TCAGTGATGC CCAACAGCAA CAGCATCCTG GATTCTCGGC CGTACCACAA 
CAGGCAGCGC CATTTATACA ACAGCCCACA CAGCAACCCA TTCAGCTATC AATGCCTTTG 
GAACAACAAT TGCAGCAATT GCTGCATAGC CAGCCAGCCC AGCAACAACA GGCCATGAGC 
CAACAGCAGC AACAGCCCTT GGTGCAACAG CAACAACTGC CTTTGGTACA ACAGCAGCCG 
CCTTTGGTCC AGCAGCAGCA ACCGCTGGTT CAGCATCAGC AGCCTTCGGT CCAACATCAG 
CAACCTTTGG TGCAACAGCC CCAGCAACAG CAACCACAGC CCCAGAATCA GCAACCACAG 
CCCCAGACAC AGCAAACACA CGTGGTTCAG CAACAACCAC CACAGCAGCA ACCTGCAGTA 
GAGCAAATCT CACAGATTTC TTCGCAAGTG CCTGTTCAAC AGGAAAATCT GCAGCCAATA 
CAGGTCAACA AAGATGCGAA TGTCGCAACG GATGCAATGT CCCTAAATAG CGCCCACGGT 
GCCCTCGAAC CTGCACCGAA GACCGAACCC CAGAACTCTG CTGATGCGGA AAAGCAGCAA 
AAGCAGACAG GCACGGGCAC ACGTTCCCAG AAACCGAGGC GATCAAACCG GAGCGGCAAC 
GAACGGATTC CCAAACTTAG TGTAACCAGC GTCGACGAGG GCAGCGTCAT AAACTGTCAC 
ATGGAAAATA AACTTAAAAC GATTACATTT AAATTCGACA TAGGAGACGT AAATCCGGTT 
GAAATAGCCA ATAAATTGAT CGCCCAAGAT TTGCTTTCTA ATTGTCAAAG TACAGTTTTT 
GTGGAAATGA TCAATGAAAT TGTTGACCAA GTCAAACAGA ATCCCAATCA AATCCCTATA 
CCGACAAATT ATCGCCGCAA TATTGAAAAG CGGGATGAAA CCGCCAGCGA CATCACAAAG 
ATGTTCGAGC CCACTATCCA TGGTGTTGAA ACCCTGCCAT CCGGTGGAGG AGGCGCTGAA 
CAGTCAAACT GCAACTTGAC TTTAGAGGCC AGGTCCCATT TATCCAACAT ACCAAATGCG 
AAAGAACCAC AGCTGAACGT CAGCACTCCG CCCACAACCA CTTCGACTAT GTCTTCCTCA 
TCAACCGCAT CGAGGGATGC GCCCAATAGT AGCAATGACG TGACCATCGG CTCTGGTTCC 
GTTTCTCGAA AAACGAGCAC GGCCTCGGAG TACACTTCGC TATCGATTGA CTATATGCCC 
GACAGCAACA TTACTCCAAC TGGTCCGGAG CCACCGCTTG ACGATGGCAA GGATAAAAAA 
CCTTGCTCCC TTGCTATAGC CAGAATGCAA AAGCTCTTGG AGTCGGCCGG CAATGGTGCT 
CCTCGCAGCC TGAATTTGCC CTTGAATCGT CACCTGAAAA TTCAAGAGGA CTTGAAGCAT 
ACTAGAAGTC TTGACGATTT GACCGCAGTG AAAATAACCT TTGACATGCC CAACAAGGCT 
GCATTGGACA CTTCGGAAAA CGCTCAGCAG GCCACAGAAG CGGAAGCGGA GAAACCAAAA 
GATAAATCCG GACAGGCAGT AGGCAACCAG GGAACTGGAG CGGCCAACAC TCTAGAGCAG 
TTAAAAATAG AACTGGAGAA CATTACTCAT GCCCACGCGT TTGCCTCCGC TGTAGTGGCC 
TCGATTAACA ACAGAGCTCC TCATCAAGCT TCCCCAGCAA TGTCCTCCCT AAAGGCCACT 
TCTGGACAAC CACAGACACA AGAGATAACC AAGCCAAATA ACGGAGCAGG CCCTTCAGTA 
CCCTCAGTGG GTCAGAATAC TCCCACTGCA GCCTTAACAT CCGCCAGGGG CTCAGGCTCC 
TCCGTCTACA ACTCACGTCG CACATCCATC GACAACAGCG TGGGATCCGA CATGCACCTG 
CACACTGCCA CTAATCTTGA AGGGACAGTG AGCAACCCGG ATCCGATCCC GACTGAGGCA 
TCGGTTGGAA TTACCATTGC TACGGGTCAT GAGAAGCAAC TCTCCAAGCA GCCCAGTTTG 
GAAAAGCCTT CTGCCACTTC CATTTTAACG AACAACAGTG ATCCTCCGCA AAGGAATCCC 
AGCAATGGAT CAATTAACCA AAATTCAATA GCTGATTTGG AAAAGAAATT GGCAGCTTTG 
CGAAATACCG AAAACACAGA AGAGTCTGCA ACAGCAACGT TGTCAGTGGT TCCTAAACAA 
GTTGAAGAGG TAGTCAATCC AAGCGCGCGT AAAATTTCGC GTTTTAGTGT CAGTCGTGTG 
CAGGAGCAGA AAACTTCAAC CGGAGTTGAG GAACCAGCTC AAGGTCAACT TAAGATCGAC 
CTCCAAGTCG CCGGCCCTGG AGGCCAGGTG CAGACCAACA ACTCCGTTCA GAACGGCAGC 
GTGGTCAACA CTCCAACCGA AGTCATCAGC TCTCCTATTC AAAATGTTCC GCTGGCCATT 
AACGGAATTC AGTTGATGTA CCAACAGCCC CAGCAGATAC ACCAGTTGCC AGGTGGAACA 
TCAACTACTA CAAGTGGAGC AGGGACTCAG TGCATTGTAG TGCCGCAAGT GGTCAATCAA 
AATGGCACCC ACGTGCAACA GCCGTCTAAC CTACAGCCAC AGCAGCAGAG CGTACATCCA 
AATATGACTC AACAGCCACA GCAGACGCCA CTTAATGGGC ACCCATCTAT GGTTAACACT 
CTTCAGCAGC AGCCACCACA ACAATCCTTA CCGATGCAGA CGATTCAGTC GCAGCAGCAG 
CAGCATAACC AAATGCCTAT CATTTCGCAA CAACAGCAGC AGCAGATCCT AATGCAGCAG 
CAACAGCAAC AGGGATCCCA GCAAGGATCA CAGCAATTTA ATTTGCCTGG CACACAACAG 
ACTCATCCTC AGCACCAGTT CATCCAGTCG CAGCCCAACC AACTTCACTC GCTTCCGCCC 
CAGGTAGTGA GCATGGCTCA GGGAAATCTG CCGCATCAGC TTCCACCCAT GCAGACTATG 
GGAGCCCAAC AGCAAATGAT CTCGCAACAA CAGCATCAGT TGCAGATGCA GTCGCATATG 
CACCAACAAA ACGTGCCCGG AATGTACAAC CAGCAGACTG GAGCTCGTGT GGCAGCACCC 
CAAAATTTCG CAGGTGCAGT TCCAAATCAT TTGTTGCAGC AGTCCCCTTT GATGGCATCC 
CAGCAGCCAG CACAGCCCAT GCAACACGTT ATGCAACCGA TTTTCAATGC AACGTCCGGT 
GAAGTTACCG AAGAGCCTGT CTCTTTAGCA GCTACGCATC CGCATCTGCT ACCGAGCGAT 
ATTCAATCCG ATATAAAGCA CAATTTAGAC TCATTAGTGA ACCAATTGTG CAATACGCGC 
TTGGGTACTA ACCAGCACCA ACGGCTGTTG CTGTTGCGTC AGAGACAACT CATCGAAGAG 
GACGAGCTGC GTCTTAAACA TTATGTGGAA TACGAAAAGT TCCAAAAGGC ACTGCGCCAA 
TCTATTAGCA CAAACGTTCC AGCTAATGCA GCTTACTATT CTGCGGCTGC CGCACAGCTT 
CCAACTAATT TGGCAGCCCC AGCAGCTCCG TCCTCATCGA ATCCGACGTC CACTAGCTCC 
GCAAACACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Wnk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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