SAK cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	SAK
Entrez Gene ID	40384
Full Name	Sak kinase
Synonyms	CG7186,DmSAK,Dmel\CG7186,PLK4,Plk4,SAK/PLK,SAK/PLK4,Sak,Sak/Plk4,plk4,sak
General protein information	Preferred Names Sak kinase Names CG7186 gene product from transcript CG7186-RA CG7186-PA Polo-like kinase 4 SAK-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78D4-78D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_141067.3	NP_649324.1	Sak kinase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLK4	XP_517431.4
Rattus norvegicus (Norway rat)	Plk4	NP_001101139.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012499	XP_307136.4
	PLK4	XP_001105577.1
Mus musculus (house mouse)	Plk4	NP_035625.2
Canis lupus familiaris (dog)	PLK4	XP_533295.2
Bos taurus (cattle)	PLK4	NP_001076896.1
Danio rerio (zebrafish)	plk4	NP_001112364.1
Drosophila melanogaster (fruit fly)	SAK	NP_649324.1
Xenopus tropicalis (tropical clawed frog)	plk4	NP_001122128.1
Homo sapiens (human)	PLK4	NP_055079.3
Gallus gallus (chicken)	LOC422497	XP_004941066.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

When Asl reduction is attenuated by Asl overexpression, plk4 mutations, Plk4 RNAi, or Slimb overexpression, Asl levels are higher in spermatozoa, resulting in embryos with reduced viability.
Title: Asterless Reduction during Spermiogenesis Is Regulated by Plk4 and Is Essential for Zygote Development in Drosophila.

Drosophila Plk4 phosphorylates four conserved serines in the STAN motif of the core centriole protein Ana2 to enable it to bind and recruit its Sas6 partner.
Title: Plk4 phosphorylates Ana2 to trigger Sas6 recruitment and procentriole formation.

Plk4 directly generates its own phosphodegron.Phosphorylation of only S293 of the Slimb-recognition motif is required for Slimb binding to Plk4.
Title: Polo-like kinase 4 autodestructs by generating its Slimb-binding phosphodegron.

Regulation of autophosphorylation controls PLK4 self-destruction and centriole number.PLK4 protein levels are controlled by Slimb.
Title: Regulation of autophosphorylation controls PLK4 self-destruction and centriole number.

Data indicate that interphase centrioles are closely associated with Sas-4, Spd-2, Polo kinase, Pericentrin-like protein (Dplp), Asterless (Asl), Plk4 kinase, Centrosomin (Cnn) and gamma-tubulin.
Title: Structured illumination of the interface between centriole and peri-centriolar material.

The following SAK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SAK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20331	NM_141067.3 Latest version!	Drosophila melanogaster Sak kinase (SAK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SAK ( NM_141067.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649324.1 Drosophila melanogaster Sak kinase (SAK), mRNA.

Price & Availability↑

CloneID	OFa20331
Clone ID Related Accession (Same CDS sequence)	NM_141067.3
Accession Version	NM_141067.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sak kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_141067.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTATCCA ATCGGGCGTT TGGAGAAACA ATTGAGGACT ATGAAGTACA GCACTTGCTG 
GGTAAAGGTG GTTTTGCGAC CGTCTACAAG GCGCGGTGCC TGCACACTCA CCAGGATGTG 
GCCATAAAGA TGATCGATAA AAAACTAATC CAGGGCACTG GACTCACTAA CCGCGTTCGC 
CAGGAAGTGG AAATCCACTC CCGCCTGAAG CATCCCTCTG TGCTTCAGCT GTACACTTTC 
TTTCAGGACG CCAACTATGT GTATTTGGTG CTCGAGCTGG CCCATAATGG GGAGCTTCAT 
CGCTATATGA ACCACATCGC CAGACCCTTC ACAGAGACGG AAGCTGCTTC CATTCTGAAG 
CAGGTGGTGG CGGGACTTTT GTACCTGCAC TCCCACAACA TCATGCACCG CGACATTTCG 
CTGTCCAACC TACTGCTTAG CAGGGAAATG CACGTCAAGA TAGCCGATTT TGGACTGGCC 
ACCCAATTAA AGCGACCTGA TGAGCGCCAT ATGACCATGT GTGGAACTCC GAACTATATT 
TCGCCTGAGG TGGTATCGCG AACGTCTCAC GGACTGCCCG CGGACGTCTG GAGCGTTGGA 
TGCATGCTGT ACACCCTGCT GGTTGGACGC CCGCCCTTCG AAACCGATGC AGTGCAGTCA 
ACCCTTAACA AAGTTGTGAT GTCCGAGTAC ATTATGCCGG CTCACTTGTC TTACGAGGCG 
CAGGACCTGA TAAACAAATT ATTAAAAAAG CTCCCGCACG AACGAATTAC CCTGGAGGCT 
GTTCTCTGCC ATCCCTTTAT GTTGAAATGC TCAAATGGTG GACACTCTGC GCCGGGAGCG 
TTGAATGTGT TCAGCCAGAG TATGGAAAGT GGCGATAGTG GAATCATAAC ATTTGCTAGC 
AGTGACTCCC GGAATTCCCA GCAAATTCGA TCTGTGGAAA ACTCAGGACC ACAACAAGTG 
CTTCCTCAAA TACGGGAGGA ATTTAAGCAA GTCCATCATA AATTGCCTTA TGAACAGACA 
GGTTTATTTG GGCAAGCTTC AACTGGCTTG GCAGAGCCAA ATTGGCCAGG AGCTGCTAAG 
TCCTCAGCGT TCTGCATGGA GGCGGGCAAT GTACCCAATT CCAAACAGGC ATCTCTTAAG 
GAAGATCGCA TTTCGGTGCC ACCATTGAAT ACTAAAAGAC TGCTTCCGAC GCGTTATAAG 
ACGAAGAATG CTATAATGAG TATTTTGCGC AATGGAGAGG TAGTTCTCGA GTTTCTTAAG 
TTTCGGCCAA CCTATAATGA AGATCGCATT AATGATATTT GTCGCATATC GGATGACGGA 
CAGCGAATCA TAATTTATCA ACCAGATCCT GGGCGTGGCT TACCAGTGAG AGAGCAGCCA 
CCAGATCTTC AAATACCAAG CGGAGATTGT GTATATAACT ACGATAATTT GCCCAGCAAG 
CACTGGAAAA AATACATATA CGGAGCTCGA TTTGTAGGGC TGGTAAAAAG CAAAACACCA 
AAAGTCACAT ACTTCAGTAC TCTAGGCAAA TGCCAATTGA TGGAGACAAT GACCGACTTT 
GAGATTCGTT TCTACTCGGG AGCTAAGCTA CTAAAGACGC CGTCCGAGGG GCTAAAGGTC 
TACGATCGAA ATGGAATGTT GCTCTCGGAT TATTCCTGTT CGGAGTCACG ATCCCTAATA 
GAGCACGGAA ACGAGTGTTT CACCCACTGT GTCAACATCA GTAATGCCTT GGAAGTAGCT 
CAGACGAAAG ATAATTCGTG TTTTCCAGTC ACAATCGGAC GAAGACCTAT AACAGATGTA 
CAACCCGCTC AGAGATTGGA TGGCCTACGA GATACCACCA ATATTGCGTT TTCCACACCA 
AAATCTAATC AGGGCTCAAT AAACTTTTCC TTAAGCACTA TTTCGTCGAC TCGAAATACT 
TCAGATTTTG GAACCAACTG CTCTAGGTCC AACATGCTAG CCGCCCATCA AAATATACCC 
ATCAAACGCA TAAACGTTCC TGAAATCGGA ATAGCTACTG AGCTCTCCCA TGGAGTTGTC 
CAAGTGCAAT TTTATGATGG ATCCGTGGTC TCTGTTATTC CGAGCATGCA AGGTGGTGGT 
ATAACCTATA CCCAACCCAA CGGAACTTCA ACCCACTTCG GCAAGGGCGA CGACTTGCCA 
TTCCCGGTCA GAGATAGAGT CGGTCAGATA CCAAACATTC AGTTAAAGTT GAAGACAGCT 
CCATTGCTGG GAAGCGGCAG GAAAACTGAC TACAACAATG CAATGACTCC CAAAACGACA 
ACGCCTTATT ATAATCGCAT GCTTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649324.1
CDS	137..2446
Translation	MLSNRAFGETIEDYEVQHLLGKGGFATVYKARCLHTHQDVAIKMIDKKLIQGTGLTNRVRQEVEIHSRLKHPSVLQLYTFFQDANYVYLVLELAHNGELHRYMNHIARPFTETEAASILKQVVAGLLYLHSHNIMHRDISLSNLLLSREMHVKIADFGLATQLKRPDERHMTMCGTPNYISPEVVSRTSHGLPADVWSVGCMLYTLLVGRPPFETDAVQSTLNKVVMSEYIMPAHLSYEAQDLINKLLKKLPHERITLEAVLCHPFMLKCSNGGHSAPGALNVFSQSMESGDSGIITFASSDSRNSQQIRSVENSGPQQVLPQIREEFKQVHHKLPYEQTGLFGQASTGLAEPNWPGAAKSSAFCMEAGNVPNSKQASLKEDRISVPPLNTKRLLPTRYKTKNAIMSILRNGEVVLEFLKFRPTYNEDRINDICRISDDGQRIIIYQPDPGRGLPVREQPPDLQIPSGDCVYNYDNLPSKHWKKYIYGARFVGLVKSKTPKVTYFSTLGKCQLMETMTDFEIRFYSGAKLLKTPSEGLKVYDRNGMLLSDYSCSESRSLIEHGNECFTHCVNISNALEVAQTKDNSCFPVTIGRRPITDVQPAQRLDGLRDTTNIAFSTPKSNQGSINFSLSTISSTRNTSDFGTNCSRSNMLAAHQNIPIKRINVPEIGIATELSHGVVQVQFYDGSVVSVIPSMQGGGITYTQPNGTSTHFGKGDDLPFPVRDRVGQIPNIQLKLKTAPLLGSGRKTDYNNAMTPKTTTPYYNRMLL

Target ORF information:

RefSeq Version	NM_141067.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sak kinase (SAK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141067.3

ORF Insert Sequence:

ATGTTATCCA ATCGGGCGTT TGGAGAAACA ATTGAGGACT ATGAAGTACA GCACTTGCTG 
GGTAAAGGTG GTTTTGCGAC CGTCTACAAG GCGCGGTGCC TGCACACTCA CCAGGATGTG 
GCCATAAAGA TGATCGATAA AAAACTAATC CAGGGCACTG GACTCACTAA CCGCGTTCGC 
CAGGAAGTGG AAATCCACTC CCGCCTGAAG CATCCCTCTG TGCTTCAGCT GTACACTTTC 
TTTCAGGACG CCAACTATGT GTATTTGGTG CTCGAGCTGG CCCATAATGG GGAGCTTCAT 
CGCTATATGA ACCACATCGC CAGACCCTTC ACAGAGACGG AAGCTGCTTC CATTCTGAAG 
CAGGTGGTGG CGGGACTTTT GTACCTGCAC TCCCACAACA TCATGCACCG CGACATTTCG 
CTGTCCAACC TACTGCTTAG CAGGGAAATG CACGTCAAGA TAGCCGATTT TGGACTGGCC 
ACCCAATTAA AGCGACCTGA TGAGCGCCAT ATGACCATGT GTGGAACTCC GAACTATATT 
TCGCCTGAGG TGGTATCGCG AACGTCTCAC GGACTGCCCG CGGACGTCTG GAGCGTTGGA 
TGCATGCTGT ACACCCTGCT GGTTGGACGC CCGCCCTTCG AAACCGATGC AGTGCAGTCA 
ACCCTTAACA AAGTTGTGAT GTCCGAGTAC ATTATGCCGG CTCACTTGTC TTACGAGGCG 
CAGGACCTGA TAAACAAATT ATTAAAAAAG CTCCCGCACG AACGAATTAC CCTGGAGGCT 
GTTCTCTGCC ATCCCTTTAT GTTGAAATGC TCAAATGGTG GACACTCTGC GCCGGGAGCG 
TTGAATGTGT TCAGCCAGAG TATGGAAAGT GGCGATAGTG GAATCATAAC ATTTGCTAGC 
AGTGACTCCC GGAATTCCCA GCAAATTCGA TCTGTGGAAA ACTCAGGACC ACAACAAGTG 
CTTCCTCAAA TACGGGAGGA ATTTAAGCAA GTCCATCATA AATTGCCTTA TGAACAGACA 
GGTTTATTTG GGCAAGCTTC AACTGGCTTG GCAGAGCCAA ATTGGCCAGG AGCTGCTAAG 
TCCTCAGCGT TCTGCATGGA GGCGGGCAAT GTACCCAATT CCAAACAGGC ATCTCTTAAG 
GAAGATCGCA TTTCGGTGCC ACCATTGAAT ACTAAAAGAC TGCTTCCGAC GCGTTATAAG 
ACGAAGAATG CTATAATGAG TATTTTGCGC AATGGAGAGG TAGTTCTCGA GTTTCTTAAG 
TTTCGGCCAA CCTATAATGA AGATCGCATT AATGATATTT GTCGCATATC GGATGACGGA 
CAGCGAATCA TAATTTATCA ACCAGATCCT GGGCGTGGCT TACCAGTGAG AGAGCAGCCA 
CCAGATCTTC AAATACCAAG CGGAGATTGT GTATATAACT ACGATAATTT GCCCAGCAAG 
CACTGGAAAA AATACATATA CGGAGCTCGA TTTGTAGGGC TGGTAAAAAG CAAAACACCA 
AAAGTCACAT ACTTCAGTAC TCTAGGCAAA TGCCAATTGA TGGAGACAAT GACCGACTTT 
GAGATTCGTT TCTACTCGGG AGCTAAGCTA CTAAAGACGC CGTCCGAGGG GCTAAAGGTC 
TACGATCGAA ATGGAATGTT GCTCTCGGAT TATTCCTGTT CGGAGTCACG ATCCCTAATA 
GAGCACGGAA ACGAGTGTTT CACCCACTGT GTCAACATCA GTAATGCCTT GGAAGTAGCT 
CAGACGAAAG ATAATTCGTG TTTTCCAGTC ACAATCGGAC GAAGACCTAT AACAGATGTA 
CAACCCGCTC AGAGATTGGA TGGCCTACGA GATACCACCA ATATTGCGTT TTCCACACCA 
AAATCTAATC AGGGCTCAAT AAACTTTTCC TTAAGCACTA TTTCGTCGAC TCGAAATACT 
TCAGATTTTG GAACCAACTG CTCTAGGTCC AACATGCTAG CCGCCCATCA AAATATACCC 
ATCAAACGCA TAAACGTTCC TGAAATCGGA ATAGCTACTG AGCTCTCCCA TGGAGTTGTC 
CAAGTGCAAT TTTATGATGG ATCCGTGGTC TCTGTTATTC CGAGCATGCA AGGTGGTGGT 
ATAACCTATA CCCAACCCAA CGGAACTTCA ACCCACTTCG GCAAGGGCGA CGACTTGCCA 
TTCCCGGTCA GAGATAGAGT CGGTCAGATA CCAAACATTC AGTTAAAGTT GAAGACAGCT 
CCATTGCTGG GAAGCGGCAG GAAAACTGAC TACAACAATG CAATGACTCC CAAAACGACA 
ACGCCTTATT ATAATCGCAT GCTTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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SAK cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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