Aef1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aef1
Entrez Gene ID	40370
Full Name	Adult enhancer factor 1
Synonyms	AEF-1,AEF1,CG5683,Dmel\CG5683
General protein information	Preferred Names Adult enhancer factor 1 Names CG5683 gene product from transcript CG5683-RD Adh enhancer factor 1 Aef1-PA Aef1-PB Aef1-PC Aef1-PD CG5683-PA CG5683-PB CG5683-PC CG5683-PD adult enhancer factor 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78D2-78D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_168904.3	NP_730631.1	adult enhancer factor 1, isoform C
NM_001275228.1	NP_001262157.1	adult enhancer factor 1, isoform D
NM_168903.3	NP_730630.1	adult enhancer factor 1, isoform A
NM_079476.3	NP_524200.1	adult enhancer factor 1, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Aef1	NP_730631.1
Canis lupus familiaris (dog)	LOC100688087	XP_005642385.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Aef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19201	NM_001275228.1 Latest version!	Drosophila melanogaster adult enhancer factor 1, transcript variant D (Aef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa20318	NM_079476.3 Latest version!	Drosophila melanogaster adult enhancer factor 1, transcript variant B (Aef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
OFa20318	NM_168903.3 Latest version!	Drosophila melanogaster adult enhancer factor 1, transcript variant A (Aef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
OFa20318	NM_168904.3 Latest version!	Drosophila melanogaster adult enhancer factor 1, transcript variant C (Aef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aef1 ( NM_001275228.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262157.1 Drosophila melanogaster adult enhancer factor 1, transcript variant D (Aef1), mRNA.

Price & Availability↑

CloneID	OFa19201
Clone ID Related Accession (Same CDS sequence)	NM_001275228.1
Accession Version	NM_001275228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	adult enhancer factor 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCATA TCAAAAGCCT GCCCCATGCC CATGCCGCTG CTACGGCGAT GAGCAGTAAC 
TGCGACATTG TCATAGTGGC CGCCCAGCCC CAAACCACCA TAGCAAACAA TAATAACAAT 
GAGACGGTCA CCCAGGCCAC GCATCCGGCT CACATGGCGG CGGTTCAACA GCAGCAGCAA 
CAACAACAGC AACAGCAGCA GCAACATCAT CAGCAGCAGC AACAGCAGTC GAGCGGACCA 
CCCTCTGTGC CGCCGCCACC CACGGAACTC CCCTTGCCCT TCCAGATGCA CTTAAGCGGG 
ATCTCGGCGG AGGCGCATAG TGCCGCCCAA GCAGCGGCTA TGGCAGCTGC CCAAGCTGCA 
GCAGCCCAAG CTGCTGCGGC GGAACAACAG CAACCGCCGC CACCAACTTC ACATCTGACG 
CACCTGACCA CGCATAGTCC GACAACGATC CACAGTGAGC ATTACCTGGC CAATGGACAT 
AGCGAGCATC CCGGAGAGGG CAACGCTGCC GTTGGCGTAG GGGGAGCTGT CCGCGAGCCG 
GAGAAACCTT TCCACTGCAC CGTCTGCGAT CGTCGCTTTC GACAGCTAAG CACACTGACC 
AACCATGTAA AGATCCATAC TGGCGAGAAG CCCTACAAAT GCAACGTTTG TGATAAGACC 
TTCCGTCAAT CGTCGACGCT GACGAACCAT CTGAAGATCC ATACGGGCGA GAAGCCTTAT 
AACTGCAACT TTTGTCCCAA GCATTTTCGA CAACTGAGCA CGCTGGCCAA CCATGTGAAG 
ATCCACACGG GTGAAAAGCC TTTTGAGTGT GTCATCTGCA AGAAGCAGTT CCGACAGTCC 
AGCACGCTCA ACAACCACAT AAAAATACAC GTCATGGACA AAGTCTACGT TCCTGTTAAG 
ATCAAAACAG AGGAGGATGA GGGGTGACTA GGACGAATGC CGTTGGCGGC GCACCACCAT 
CAGCAGCAGC AACACCAGCA CCACCATCAA CAGCAGCAGC ATCAGCACCA CCCGCCGCCT 
CCGTCCCAGC AGCAGCAGCA GCATATCGAT CAGCGTGGAG TCACCATAAC CACGTTGCCC 
TCCGCCACGA CAGTGGCCCA ACATCACCAG CAGCATCAGC ACATTCAGGA TGGCTCGCCG 
CACCATCATT TTAATGTGGC CGCTCTAGGG GACTTATCCA GCGCTATGCA ATTGGGCGCT 
GTGACGGCGG ACGGGAGCTT TGTGACCATG GGCGGTGTTG TGGTGGGTCG CATCCAGCAC 
ACACGAGAGG AACTGCAGCA GCTTGGAGTG ATCAAGGTGG AGTCACCATC GAATGGTGGC 
ACAGCCACGA CGTCAGCGAC GGCGGAAACG CAACGCGAGG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262157.1
CDS	476..1840
Translation	MMHIKSLPHAHAAATAMSSNCDIVIVAAQPQTTIANNNNNETVTQATHPAHMAAVQQQQQQQQQQQQQHHQQQQQQSSGPPSVPPPPTELPLPFQMHLSGISAEAHSAAQAAAMAAAQAAAAQAAAAEQQQPPPPTSHLTHLTTHSPTTIHSEHYLANGHSEHPGEGNAAVGVGGAVREPEKPFHCTVCDRRFRQLSTLTNHVKIHTGEKPYKCNVCDKTFRQSSTLTNHLKIHTGEKPYNCNFCPKHFRQLSTLANHVKIHTGEKPFECVICKKQFRQSSTLNNHIKIHVMDKVYVPVKIKTEEDEG*LGRMPLAAHHHQQQQHQHHHQQQQHQHHPPPPSQQQQQHIDQRGVTITTLPSATTVAQHHQQHQHIQDGSPHHHFNVAALGDLSSAMQLGAVTADGSFVTMGGVVVGRIQHTREELQQLGVIKVESPSNGGTATTSATAETQREG

Target ORF information:

RefSeq Version	NM_001275228.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adult enhancer factor 1, transcript variant D (Aef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275228.1

ORF Insert Sequence:

ATGATGCATA TCAAAAGCCT GCCCCATGCC CATGCCGCTG CTACGGCGAT GAGCAGTAAC 
TGCGACATTG TCATAGTGGC CGCCCAGCCC CAAACCACCA TAGCAAACAA TAATAACAAT 
GAGACGGTCA CCCAGGCCAC GCATCCGGCT CACATGGCGG CGGTTCAACA GCAGCAGCAA 
CAACAACAGC AACAGCAGCA GCAACATCAT CAGCAGCAGC AACAGCAGTC GAGCGGACCA 
CCCTCTGTGC CGCCGCCACC CACGGAACTC CCCTTGCCCT TCCAGATGCA CTTAAGCGGG 
ATCTCGGCGG AGGCGCATAG TGCCGCCCAA GCAGCGGCTA TGGCAGCTGC CCAAGCTGCA 
GCAGCCCAAG CTGCTGCGGC GGAACAACAG CAACCGCCGC CACCAACTTC ACATCTGACG 
CACCTGACCA CGCATAGTCC GACAACGATC CACAGTGAGC ATTACCTGGC CAATGGACAT 
AGCGAGCATC CCGGAGAGGG CAACGCTGCC GTTGGCGTAG GGGGAGCTGT CCGCGAGCCG 
GAGAAACCTT TCCACTGCAC CGTCTGCGAT CGTCGCTTTC GACAGCTAAG CACACTGACC 
AACCATGTAA AGATCCATAC TGGCGAGAAG CCCTACAAAT GCAACGTTTG TGATAAGACC 
TTCCGTCAAT CGTCGACGCT GACGAACCAT CTGAAGATCC ATACGGGCGA GAAGCCTTAT 
AACTGCAACT TTTGTCCCAA GCATTTTCGA CAACTGAGCA CGCTGGCCAA CCATGTGAAG 
ATCCACACGG GTGAAAAGCC TTTTGAGTGT GTCATCTGCA AGAAGCAGTT CCGACAGTCC 
AGCACGCTCA ACAACCACAT AAAAATACAC GTCATGGACA AAGTCTACGT TCCTGTTAAG 
ATCAAAACAG AGGAGGATGA GGGGTGACTA GGACGAATGC CGTTGGCGGC GCACCACCAT 
CAGCAGCAGC AACACCAGCA CCACCATCAA CAGCAGCAGC ATCAGCACCA CCCGCCGCCT 
CCGTCCCAGC AGCAGCAGCA GCATATCGAT CAGCGTGGAG TCACCATAAC CACGTTGCCC 
TCCGCCACGA CAGTGGCCCA ACATCACCAG CAGCATCAGC ACATTCAGGA TGGCTCGCCG 
CACCATCATT TTAATGTGGC CGCTCTAGGG GACTTATCCA GCGCTATGCA ATTGGGCGCT 
GTGACGGCGG ACGGGAGCTT TGTGACCATG GGCGGTGTTG TGGTGGGTCG CATCCAGCAC 
ACACGAGAGG AACTGCAGCA GCTTGGAGTG ATCAAGGTGG AGTCACCATC GAATGGTGGC 
ACAGCCACGA CGTCAGCGAC GGCGGAAACG CAACGCGAGG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aef1 ( NM_168904.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730631.1 Drosophila melanogaster adult enhancer factor 1, transcript variant C (Aef1), mRNA.

Price & Availability↑

CloneID	OFa20318
Clone ID Related Accession (Same CDS sequence)	NM_168904.3 , NM_168903.3 , NM_079476.3
Accession Version	NM_168904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	adult enhancer factor 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168904.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCATA TCAAAAGCCT GCCCCATGCC CATGCCGCTG CTACGGCGAT GAGCAGTAAC 
TGCGACATTG TCATAGTGGC CGCCCAGCCC CAAACCACCA TAGCAAACAA TAATAACAAT 
GAGACGGTCA CCCAGGCCAC GCATCCGGCT CACATGGCGG CGGTTCAACA GCAGCAGCAA 
CAACAACAGC AACAGCAGCA GCAACATCAT CAGCAGCAGC AACAGCAGTC GAGCGGACCA 
CCCTCTGTGC CGCCGCCACC CACGGAACTC CCCTTGCCCT TCCAGATGCA CTTAAGCGGG 
ATCTCGGCGG AGGCGCATAG TGCCGCCCAA GCAGCGGCTA TGGCAGCTGC CCAAGCTGCA 
GCAGCCCAAG CTGCTGCGGC GGAACAACAG CAACCGCCGC CACCAACTTC ACATCTGACG 
CACCTGACCA CGCATAGTCC GACAACGATC CACAGTGAGC ATTACCTGGC CAATGGACAT 
AGCGAGCATC CCGGAGAGGG CAACGCTGCC GTTGGCGTAG GGGGAGCTGT CCGCGAGCCG 
GAGAAACCTT TCCACTGCAC CGTCTGCGAT CGTCGCTTTC GACAGCTAAG CACACTGACC 
AACCATGTAA AGATCCATAC TGGCGAGAAG CCCTACAAAT GCAACGTTTG TGATAAGACC 
TTCCGTCAAT CGTCGACGCT GACGAACCAT CTGAAGATCC ATACGGGCGA GAAGCCTTAT 
AACTGCAACT TTTGTCCCAA GCATTTTCGA CAACTGAGCA CGCTGGCCAA CCATGTGAAG 
ATCCACACGG GTGAAAAGCC TTTTGAGTGT GTCATCTGCA AGAAGCAGTT CCGACAGTCC 
AGCACGCTCA ACAACCACAT AAAAATACAC GTCATGGACA AAGTCTACGT TCCTGTTAAG 
ATCAAAACAG AGGAGGATGA GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730631.1
CDS	414..1340
Translation	MMHIKSLPHAHAAATAMSSNCDIVIVAAQPQTTIANNNNNETVTQATHPAHMAAVQQQQQQQQQQQQQHHQQQQQQSSGPPSVPPPPTELPLPFQMHLSGISAEAHSAAQAAAMAAAQAAAAQAAAAEQQQPPPPTSHLTHLTTHSPTTIHSEHYLANGHSEHPGEGNAAVGVGGAVREPEKPFHCTVCDRRFRQLSTLTNHVKIHTGEKPYKCNVCDKTFRQSSTLTNHLKIHTGEKPYNCNFCPKHFRQLSTLANHVKIHTGEKPFECVICKKQFRQSSTLNNHIKIHVMDKVYVPVKIKTEEDEG

Target ORF information:

RefSeq Version	NM_168904.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adult enhancer factor 1, transcript variant C (Aef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168904.3

ORF Insert Sequence:

ATGATGCATA TCAAAAGCCT GCCCCATGCC CATGCCGCTG CTACGGCGAT GAGCAGTAAC 
TGCGACATTG TCATAGTGGC CGCCCAGCCC CAAACCACCA TAGCAAACAA TAATAACAAT 
GAGACGGTCA CCCAGGCCAC GCATCCGGCT CACATGGCGG CGGTTCAACA GCAGCAGCAA 
CAACAACAGC AACAGCAGCA GCAACATCAT CAGCAGCAGC AACAGCAGTC GAGCGGACCA 
CCCTCTGTGC CGCCGCCACC CACGGAACTC CCCTTGCCCT TCCAGATGCA CTTAAGCGGG 
ATCTCGGCGG AGGCGCATAG TGCCGCCCAA GCAGCGGCTA TGGCAGCTGC CCAAGCTGCA 
GCAGCCCAAG CTGCTGCGGC GGAACAACAG CAACCGCCGC CACCAACTTC ACATCTGACG 
CACCTGACCA CGCATAGTCC GACAACGATC CACAGTGAGC ATTACCTGGC CAATGGACAT 
AGCGAGCATC CCGGAGAGGG CAACGCTGCC GTTGGCGTAG GGGGAGCTGT CCGCGAGCCG 
GAGAAACCTT TCCACTGCAC CGTCTGCGAT CGTCGCTTTC GACAGCTAAG CACACTGACC 
AACCATGTAA AGATCCATAC TGGCGAGAAG CCCTACAAAT GCAACGTTTG TGATAAGACC 
TTCCGTCAAT CGTCGACGCT GACGAACCAT CTGAAGATCC ATACGGGCGA GAAGCCTTAT 
AACTGCAACT TTTGTCCCAA GCATTTTCGA CAACTGAGCA CGCTGGCCAA CCATGTGAAG 
ATCCACACGG GTGAAAAGCC TTTTGAGTGT GTCATCTGCA AGAAGCAGTT CCGACAGTCC 
AGCACGCTCA ACAACCACAT AAAAATACAC GTCATGGACA AAGTCTACGT TCCTGTTAAG 
ATCAAAACAG AGGAGGATGA GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aef1 ( NM_168903.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730630.1 Drosophila melanogaster adult enhancer factor 1, transcript variant A (Aef1), mRNA.

Price & Availability↑

CloneID	OFa20318
Clone ID Related Accession (Same CDS sequence)	NM_168904.3 , NM_168903.3 , NM_079476.3
Accession Version	NM_168903.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	adult enhancer factor 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168903.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCATA TCAAAAGCCT GCCCCATGCC CATGCCGCTG CTACGGCGAT GAGCAGTAAC 
TGCGACATTG TCATAGTGGC CGCCCAGCCC CAAACCACCA TAGCAAACAA TAATAACAAT 
GAGACGGTCA CCCAGGCCAC GCATCCGGCT CACATGGCGG CGGTTCAACA GCAGCAGCAA 
CAACAACAGC AACAGCAGCA GCAACATCAT CAGCAGCAGC AACAGCAGTC GAGCGGACCA 
CCCTCTGTGC CGCCGCCACC CACGGAACTC CCCTTGCCCT TCCAGATGCA CTTAAGCGGG 
ATCTCGGCGG AGGCGCATAG TGCCGCCCAA GCAGCGGCTA TGGCAGCTGC CCAAGCTGCA 
GCAGCCCAAG CTGCTGCGGC GGAACAACAG CAACCGCCGC CACCAACTTC ACATCTGACG 
CACCTGACCA CGCATAGTCC GACAACGATC CACAGTGAGC ATTACCTGGC CAATGGACAT 
AGCGAGCATC CCGGAGAGGG CAACGCTGCC GTTGGCGTAG GGGGAGCTGT CCGCGAGCCG 
GAGAAACCTT TCCACTGCAC CGTCTGCGAT CGTCGCTTTC GACAGCTAAG CACACTGACC 
AACCATGTAA AGATCCATAC TGGCGAGAAG CCCTACAAAT GCAACGTTTG TGATAAGACC 
TTCCGTCAAT CGTCGACGCT GACGAACCAT CTGAAGATCC ATACGGGCGA GAAGCCTTAT 
AACTGCAACT TTTGTCCCAA GCATTTTCGA CAACTGAGCA CGCTGGCCAA CCATGTGAAG 
ATCCACACGG GTGAAAAGCC TTTTGAGTGT GTCATCTGCA AGAAGCAGTT CCGACAGTCC 
AGCACGCTCA ACAACCACAT AAAAATACAC GTCATGGACA AAGTCTACGT TCCTGTTAAG 
ATCAAAACAG AGGAGGATGA GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730630.1
CDS	470..1396
Translation	MMHIKSLPHAHAAATAMSSNCDIVIVAAQPQTTIANNNNNETVTQATHPAHMAAVQQQQQQQQQQQQQHHQQQQQQSSGPPSVPPPPTELPLPFQMHLSGISAEAHSAAQAAAMAAAQAAAAQAAAAEQQQPPPPTSHLTHLTTHSPTTIHSEHYLANGHSEHPGEGNAAVGVGGAVREPEKPFHCTVCDRRFRQLSTLTNHVKIHTGEKPYKCNVCDKTFRQSSTLTNHLKIHTGEKPYNCNFCPKHFRQLSTLANHVKIHTGEKPFECVICKKQFRQSSTLNNHIKIHVMDKVYVPVKIKTEEDEG

Target ORF information:

RefSeq Version	NM_168903.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adult enhancer factor 1, transcript variant A (Aef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168903.3

ORF Insert Sequence:

ATGATGCATA TCAAAAGCCT GCCCCATGCC CATGCCGCTG CTACGGCGAT GAGCAGTAAC 
TGCGACATTG TCATAGTGGC CGCCCAGCCC CAAACCACCA TAGCAAACAA TAATAACAAT 
GAGACGGTCA CCCAGGCCAC GCATCCGGCT CACATGGCGG CGGTTCAACA GCAGCAGCAA 
CAACAACAGC AACAGCAGCA GCAACATCAT CAGCAGCAGC AACAGCAGTC GAGCGGACCA 
CCCTCTGTGC CGCCGCCACC CACGGAACTC CCCTTGCCCT TCCAGATGCA CTTAAGCGGG 
ATCTCGGCGG AGGCGCATAG TGCCGCCCAA GCAGCGGCTA TGGCAGCTGC CCAAGCTGCA 
GCAGCCCAAG CTGCTGCGGC GGAACAACAG CAACCGCCGC CACCAACTTC ACATCTGACG 
CACCTGACCA CGCATAGTCC GACAACGATC CACAGTGAGC ATTACCTGGC CAATGGACAT 
AGCGAGCATC CCGGAGAGGG CAACGCTGCC GTTGGCGTAG GGGGAGCTGT CCGCGAGCCG 
GAGAAACCTT TCCACTGCAC CGTCTGCGAT CGTCGCTTTC GACAGCTAAG CACACTGACC 
AACCATGTAA AGATCCATAC TGGCGAGAAG CCCTACAAAT GCAACGTTTG TGATAAGACC 
TTCCGTCAAT CGTCGACGCT GACGAACCAT CTGAAGATCC ATACGGGCGA GAAGCCTTAT 
AACTGCAACT TTTGTCCCAA GCATTTTCGA CAACTGAGCA CGCTGGCCAA CCATGTGAAG 
ATCCACACGG GTGAAAAGCC TTTTGAGTGT GTCATCTGCA AGAAGCAGTT CCGACAGTCC 
AGCACGCTCA ACAACCACAT AAAAATACAC GTCATGGACA AAGTCTACGT TCCTGTTAAG 
ATCAAAACAG AGGAGGATGA GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aef1 ( NM_079476.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524200.1 Drosophila melanogaster adult enhancer factor 1, transcript variant B (Aef1), mRNA.

Price & Availability↑

CloneID	OFa20318
Clone ID Related Accession (Same CDS sequence)	NM_168904.3 , NM_168903.3 , NM_079476.3
Accession Version	NM_079476.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	adult enhancer factor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079476.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCATA TCAAAAGCCT GCCCCATGCC CATGCCGCTG CTACGGCGAT GAGCAGTAAC 
TGCGACATTG TCATAGTGGC CGCCCAGCCC CAAACCACCA TAGCAAACAA TAATAACAAT 
GAGACGGTCA CCCAGGCCAC GCATCCGGCT CACATGGCGG CGGTTCAACA GCAGCAGCAA 
CAACAACAGC AACAGCAGCA GCAACATCAT CAGCAGCAGC AACAGCAGTC GAGCGGACCA 
CCCTCTGTGC CGCCGCCACC CACGGAACTC CCCTTGCCCT TCCAGATGCA CTTAAGCGGG 
ATCTCGGCGG AGGCGCATAG TGCCGCCCAA GCAGCGGCTA TGGCAGCTGC CCAAGCTGCA 
GCAGCCCAAG CTGCTGCGGC GGAACAACAG CAACCGCCGC CACCAACTTC ACATCTGACG 
CACCTGACCA CGCATAGTCC GACAACGATC CACAGTGAGC ATTACCTGGC CAATGGACAT 
AGCGAGCATC CCGGAGAGGG CAACGCTGCC GTTGGCGTAG GGGGAGCTGT CCGCGAGCCG 
GAGAAACCTT TCCACTGCAC CGTCTGCGAT CGTCGCTTTC GACAGCTAAG CACACTGACC 
AACCATGTAA AGATCCATAC TGGCGAGAAG CCCTACAAAT GCAACGTTTG TGATAAGACC 
TTCCGTCAAT CGTCGACGCT GACGAACCAT CTGAAGATCC ATACGGGCGA GAAGCCTTAT 
AACTGCAACT TTTGTCCCAA GCATTTTCGA CAACTGAGCA CGCTGGCCAA CCATGTGAAG 
ATCCACACGG GTGAAAAGCC TTTTGAGTGT GTCATCTGCA AGAAGCAGTT CCGACAGTCC 
AGCACGCTCA ACAACCACAT AAAAATACAC GTCATGGACA AAGTCTACGT TCCTGTTAAG 
ATCAAAACAG AGGAGGATGA GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524200.1
CDS	371..1297
Translation	MMHIKSLPHAHAAATAMSSNCDIVIVAAQPQTTIANNNNNETVTQATHPAHMAAVQQQQQQQQQQQQQHHQQQQQQSSGPPSVPPPPTELPLPFQMHLSGISAEAHSAAQAAAMAAAQAAAAQAAAAEQQQPPPPTSHLTHLTTHSPTTIHSEHYLANGHSEHPGEGNAAVGVGGAVREPEKPFHCTVCDRRFRQLSTLTNHVKIHTGEKPYKCNVCDKTFRQSSTLTNHLKIHTGEKPYNCNFCPKHFRQLSTLANHVKIHTGEKPFECVICKKQFRQSSTLNNHIKIHVMDKVYVPVKIKTEEDEG

Target ORF information:

RefSeq Version	NM_079476.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster adult enhancer factor 1, transcript variant B (Aef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079476.3

ORF Insert Sequence:

ATGATGCATA TCAAAAGCCT GCCCCATGCC CATGCCGCTG CTACGGCGAT GAGCAGTAAC 
TGCGACATTG TCATAGTGGC CGCCCAGCCC CAAACCACCA TAGCAAACAA TAATAACAAT 
GAGACGGTCA CCCAGGCCAC GCATCCGGCT CACATGGCGG CGGTTCAACA GCAGCAGCAA 
CAACAACAGC AACAGCAGCA GCAACATCAT CAGCAGCAGC AACAGCAGTC GAGCGGACCA 
CCCTCTGTGC CGCCGCCACC CACGGAACTC CCCTTGCCCT TCCAGATGCA CTTAAGCGGG 
ATCTCGGCGG AGGCGCATAG TGCCGCCCAA GCAGCGGCTA TGGCAGCTGC CCAAGCTGCA 
GCAGCCCAAG CTGCTGCGGC GGAACAACAG CAACCGCCGC CACCAACTTC ACATCTGACG 
CACCTGACCA CGCATAGTCC GACAACGATC CACAGTGAGC ATTACCTGGC CAATGGACAT 
AGCGAGCATC CCGGAGAGGG CAACGCTGCC GTTGGCGTAG GGGGAGCTGT CCGCGAGCCG 
GAGAAACCTT TCCACTGCAC CGTCTGCGAT CGTCGCTTTC GACAGCTAAG CACACTGACC 
AACCATGTAA AGATCCATAC TGGCGAGAAG CCCTACAAAT GCAACGTTTG TGATAAGACC 
TTCCGTCAAT CGTCGACGCT GACGAACCAT CTGAAGATCC ATACGGGCGA GAAGCCTTAT 
AACTGCAACT TTTGTCCCAA GCATTTTCGA CAACTGAGCA CGCTGGCCAA CCATGTGAAG 
ATCCACACGG GTGAAAAGCC TTTTGAGTGT GTCATCTGCA AGAAGCAGTT CCGACAGTCC 
AGCACGCTCA ACAACCACAT AAAAATACAC GTCATGGACA AAGTCTACGT TCCTGTTAAG 
ATCAAAACAG AGGAGGATGA GGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Aef1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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